CN102125614A - Preparation method of traditional Chinese medicine composition for treating common cold of children - Google Patents
Preparation method of traditional Chinese medicine composition for treating common cold of children Download PDFInfo
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Abstract
The invention discloses a preparation method of a traditional Chinese medicine composition for treating common cold of children. The method comprises the following steps: boiling ephedra in water; soaking gypsum, isatis root, asiatic moonseed rhizome and licorice root in water and boiling together; heating Baical Skullcap Root in boiling water; extracting bitter almond with ethanol; mixing the decoctions; filtering; concentrating the filtrate under a reduced pressure; standing; and filtering. The traditional Chinese medicine composition provided by the invention has a good curative effect on common cold.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition preparation method, particularly relate to a kind of Chinese medicine composition preparation method for the treatment of infantile common cold.
Background technology
Prior art discloses the prescription and the preparation method of infantile heat-clearing and antitussive oral liquid, is respectively:
Prescription: Herba Ephedrae 90g Semen Armeniacae Amarum (stir-fry) 120g Gypsum Fibrosum 270g
Radix Glycyrrhizae 90g Radix Scutellariae 180g Radix Isatidis 180g
Rhizoma Menispermi 90g
Method for making: Herba Ephedrae, Gypsum Fibrosum decoct with water half an hour, add the five tastes such as Semen Armeniacae Amarum again, decoct secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, filtrate decompression is concentrated into about 600ml, leaves standstill 24 hours, filters, filtrate adds Mel 200g, sucrose 100g and sodium benzoate 3g, boils to make dissolving, adds water to total amount 1000ml, stir evenly, cold preservation 24~48 hours filters, embedding, sterilization, promptly.
The present invention is the improvement that preparation method is carried out, and new product has more outstanding drug effect.
Summary of the invention
The object of the invention is to provide a kind of Chinese medicine composition preparation method for the treatment of flu.
The present invention seeks to be achieved through the following technical solutions:
A kind of preparation method for the treatment of the Chinese medicine composition of infantile common cold of the present invention comprises the steps:
Choose following crude drug:
Herba Ephedrae 70-120g Semen Armeniacae Amarum (stir-fry) 100-160g Gypsum Fibrosum 220-350g
Radix Glycyrrhizae 60-120g Radix Scutellariae 150-220g Radix Isatidis 150-220g
Rhizoma Menispermi 60-120g;
Step 1: Gypsum Fibrosum, Radix Glycyrrhizae, Rhizoma Menispermi, Radix Isatidis, decoct 1-4 time, add 4-8 times of water gaging at every turn and decocted 1-3 hour, collecting decoction filters, filtrate concentrate extractum A;
Step 2: Herba Ephedrae decocts with water 1-4 time, adds 4-8 times of water gaging at every turn and decocts 1-3 hour, and collecting decoction filters, and gets extractum B;
Step 3: Semen Armeniacae Amarum is with 70% alcohol reflux 1-3 time, adds 4-8 at every turn and doubly measures 70% ethanol and decocted 0.5-3 hour, merges backflow, reclaims ethanol to there not being the alcohol flavor, extractum C;
Step 4: Radix Scutellariae decocts with water 1-3 time, heats water to 80 ℃ earlier for the first time, drops into Radix Scutellariae then, and decoction liquor filters to merge and concentrates, and gets extractum D;
Step 5: merge extractum A, B, C, D, make various dosage forms, comprise capsule, drop pill, tablet, granule, gel, oral liquid through conventional technology.
For above-mentioned dosage form can be realized, need when these dosage forms of preparation, to add the pharmacy acceptable auxiliary, for example: filler, disintegrating agent, lubricant, suspending agent, binding agent, sweeting agent, correctives, antiseptic, substrate etc.Filler comprises: starch, pregelatinized Starch, lactose, mannitol, chitin, microcrystalline Cellulose, sucrose etc.; Disintegrating agent comprises: starch, pregelatinized Starch, microcrystalline Cellulose, carboxymethyl starch sodium, crospolyvinylpyrrolidone, low-substituted hydroxypropyl cellulose, cross-linking sodium carboxymethyl cellulose etc.; Lubricant comprises: magnesium stearate, sodium lauryl sulphate, Pulvis Talci, silicon dioxide etc.; Suspending agent comprises: polyvinylpyrrolidone, microcrystalline Cellulose, sucrose, agar, hydroxypropyl emthylcellulose etc.; Binding agent comprises, starch slurry, polyvinylpyrrolidone, hydroxypropyl emthylcellulose etc.; Sweeting agent comprises: saccharin sodium, Aspartane, sucrose, cyclamate, enoxolone etc.; Correctives comprises: sweeting agent and various essence; Antiseptic comprises: parabens, benzoic acid, sodium benzoate, sorbic acid and its esters, benzalkonium bromide, fixed, the Folium eucalypti globueli (Eucalyptus globulus Labill.) wet goods of acetic acid chloroethene; Substrate comprises: PEG6000, PEG4000, insect wax etc.
Above-mentioned preparation method preferably includes following steps:
Herba Ephedrae 90g Semen Armeniacae Amarum (stir-fry) 120g Gypsum Fibrosum 270g Radix Glycyrrhizae 90g
Radix Scutellariae 180g Radix Isatidis 180g Rhizoma Menispermi 90g
Step 1: Gypsum Fibrosum, Radix Glycyrrhizae, Rhizoma Menispermi, the Radix Isatidis five tastes, decoct secondary, add 8 times of water gagings the first time and decocted 2 hours, add 6 times of water gagings for the second time and decocted 1 hour, collecting decoction filters, and filtrate decompression is concentrated into relative density 1.05~1.06, gets extractum A;
Step 2: Herba Ephedrae decocts with water 2 times, measures decoction 2.5 hours for 8 times for the first time, and 6 times of amounts decocted 1 hour for the second time, and filtrate is concentrated into relative density 1.05~1.06, gets extractum B;
Step 3: Semen Armeniacae Amarum is with 70% alcohol reflux secondary, and each 1 hour 4 times of amount merges backflow, reclaims ethanol to there not being the alcohol flavor, extractum C;
Step 4: Radix Scutellariae decocts with water 2 times, heats water to 80 ℃ earlier for the first time, drops into Radix Scutellariae then, and twice decoction liquor filters to merge and be concentrated into relative density 1.05~1.06 filtrates, gets extractum D;
Step 5: merge extractum A, B, C, D, add Mel 200g, sucrose 100g and sodium benzoate 3g add water to total amount 1000ml, stir centrifugal behind the 50min, fill, sterilization, promptly.
Following experimental example and embodiment are used to further specify but are not limited to the present invention.
Antibacterial tests in the experimental example 1 mice body
Animal: NIH kind mice, 12-14g, male and female half and half.
Infection bacteria species: staphylococcus aureus, available from Nat'l Pharmaceutical ﹠ Biological Products Control Institute strain chamber.
Infection dosage and route of infection
1. infection dosage: 6 * 10
8/ only as infection dosage, trial test mouse death rate 85%.
2. route of infection: lumbar injection, 0.4ml/ are only.
Test drug:
Of the present invention group: the preparation of embodiment 1 method.
Matched group 1: prescription is with embodiment 1, and Herba Ephedrae, Gypsum Fibrosum decoct with water half an hour, adds the five tastes such as Semen Armeniacae Amarum again, decocts secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, filtrate decompression is concentrated into about 600ml, leaves standstill 24 hours, filters, filtrate adds Mel 200g, sucrose 100g and sodium benzoate 3g, boils to make dissolving, adds water to total amount 1000ml, stir evenly, cold preservation 24~48 hours filters, embedding, sterilization, promptly.
Test method
(1) trial test of infection dosage
Get 100 of NIH kind mices, body weight 12-14g is divided into 5 groups at random, and 20 every group, each organizes body weight does not have significant difference (P<0.05), and respectively bacterium liquid 0.4ml/ of group difference lumbar injection variable concentrations gradient, the bacterium amount is respectively 2 * 10
8/ only, 4 * 10
8/ only, 6 * 10
8/ only, 8 * 10
8/ only, 10 * 10
8/ only.Observe the death condition of animal in 15 days, infection dosage 6 * 10 as a result
8/ mortality rate is determined with 6 * 10 at last more than 80%
8/ only use infection dosage for test, the results are shown in Table 1.
Table 1 infection dosage result of the test
(2) the endogenous protective effect of infecting mouse is tested
Get 60 of NIH kind mices, body weight 12.8 ± 0.96g, male and female half and half, be divided into 3 groups at random, comprise and infect matched group, of the present invention group, matched group 1, each group gives 40.0g crude drug except that infecting matched group, each organizes weight ratio than there was no significant difference (P<0.05), before the infection, and of the present invention group, matched group 1, gastric infusion is 1 day respectively, 2 times on the one, other irritates distilled water, second day, each only organizes the lumbar injection gold bacterium liquid 0.4ml/ of Portugal, and infection dosage is 6 * 10
8/ only, and of the present invention group, matched group 1, the difference gastric infusion, a twice-daily, successive administration two days, was observed X 15 days continuously at general behavior, death toll, the death time of observing animal
2(2 * 2) method check experimental result the results are shown in Table 2.
Antibacterial tests result in table 2 body
Table 2 is the result show, of the present invention group animal dis motility rate is significantly higher than matched group 1.
Experimental example 2
Test drug:
Of the present invention group: the preparation of embodiment 1 method.
Matched group 1: prescription is with embodiment 1, and Herba Ephedrae, Gypsum Fibrosum decoct with water half an hour, adds the five tastes such as Semen Armeniacae Amarum again, decocts secondary, 2 hours for the first time, 1 hour for the second time, collecting decoction filtered, filtrate decompression is concentrated into about 600ml, leaves standstill 24 hours, filters, filtrate adds Mel 200g, sucrose 100g and sodium benzoate 3g, boils to make dissolving, adds water to total amount 1000ml, stir evenly, cold preservation 24~48 hours filters, embedding, sterilization, promptly.
Choose basic threshold value and be 10~20 seconds 40 of female mices, body weight 18~22g is divided into 2 groups at random, 20 every group, survey each Mus threshold of pain with hot plate method before the administration, of the present invention group, matched group 1, by clinical 30 times of gastric infusions with dosage, after the administration under right back sufficient plantar aponeurosis SC1% carrageenin aqueous solution 0.1ml/ Mus, after the administration 60, surveyed with method in 120 minutes and respectively organize the mice threshold of pain, carry out statistical analysis, the results are shown in Table 3 with the t check
Table 3 medicine causes the influence (x ± SD) of pain to the mice hot plate
Annotate: * compares P<0.05 for of the present invention group
The result shows: of the present invention group better than separating table function with matched group 1.
The extraction of baicalin experiment in experimental example 3 Radix Scutellariaes
1. experiment purpose:
Adjusting extracting mode on the basis of existing extraction equipment, is to investigate index with the content of baicalin, adopts different extraction conditions that radix scutellariae medicinal materials is extracted, and calculates the whole bag of tricks and extracts the rate of transform.
2. experimental technique:
2.1 medical material processing method:
Contain flavone compounds such as baicalin in the baikal skullcap root.
2.1.1 adopt burning water to extract, measure content of baicalin in the extracting solution.
Handle the method for Radix Scutellariae: get radix scutellariae medicinal materials 200g (lot number: 20081106), solvent: boiling water; Extraction time: 2h, 1.5h, 1h; 10 times of solvent loads, 8 times, 6 times, extraction time: 1 time, 2 times, 3 times.Carry out orthogonal test:
One: 10 times of water gaging of sample extracts 1 each 2h;
Two: 8 times of water gagings of sample extract 2 each 2h;
Three: 6 times of water gagings of sample extract 3 each 2h;
Four: 8 times of water gagings of sample extract 1 each 1.5h;
Five: 6 times of water gagings of sample extract 2 each 1.5h;
Six: 10 times of water gagings of sample extract 3 each 1.5h;
Seven: 6 times of water gagings of sample extract 1 each 1h;
Eight: 10 times of water gagings of sample extract 2 each 1h;
Nine: 8 times of water gagings of sample extract 3 each 1h.
2.1.2 close extraction with Semen Armeniacae Amarum, measure content of baicalin in three extracting solution.
Sample ten: get radix scutellariae medicinal materials 200g, Semen Armeniacae Amarum 130g, 10 times of water gagings extract 3 each 1.5h.
2.2 detection method:
Chromatographic condition and system suitability experiment: with the RP-18 bonded silica gel is filler; Methanol-water-phosphoric acid (47: 53: 0.2) is a mobile phase; The detection wavelength is 280nm.Theoretical cam curve is calculated by the baicalin peak and is not less than 1600.
The baicalin reference substance solution: concentration is 9.9 * 10
-2Mg/ml,
The preparation of need testing solution: the about 1g of precision weighing concentrated solution supernatant, put in the 25ml volumetric flask, add the about 20ml of 70% ethanol, supersound process makes dissolving, adds 70% ethanol to scale, shakes up, and filters and gets subsequent filtrate, promptly.
3. experimental data and analysis:
Content of baicalin average out to 11% in the medical material
3.1 each extraction method:
3.2 conclusion
Got by orthogonal experiments, two: 8 times of water gagings of sample extract 2 each 2h, and effect is best, because six: 10 times of water gagings of sample extract 3 each 1.5h, the rate of transform is also higher, and suggestion uses 8 times of water gagings to extract three times, extracts 2h at every turn.
Experimental example 4 Semen Armeniacae Amarum Study on extraction
1, amygdaloside Determination on content method:
Chromatographic condition and system suitability test Kromasil C18 (4.6*150mm, 5 μ m) chromatographic column; Acetonitrile-water (10: 90) is a mobile phase; The detection wavelength is 210nm; The preparation of amygdaloside reference substance solution: precision takes by weighing the amygdaloside reference substance 2.59mg that is dried to constant weight in silica gel drier, puts in the 10mL measuring bottle, adds dissolve with methanol and is diluted to scale, shakes up, promptly.The preparation of need testing solution: precision is measured 1.0ml (oral liquid of embodiment 1 preparation), puts in the 10ml measuring bottle,, shakes up to scale with methanol constant volume, filters, and gets subsequent filtrate, promptly.Algoscopy: the above-mentioned need testing solution 5 μ L of accurate respectively absorption inject chromatograph of liquid, measure, promptly.
2, the selection of the establishment of experimental technique and factor level thereof
Semen Armeniacae Amarum contains cyanogen glycosides and organic acid composition thereof.The unexpected concentration of alcohol of finding is 70% to carry out being better than when certificate got other concentration, therefore mainly investigates adding ethanol consumption, extraction time, three factors of extraction time, and selects three levels to test.
The factor level table
Horizontal factor | Amount of alcohol (doubly) | Extraction time (hour) | Extraction time |
1 | 8 | 2 | |
2 | 6 | 1.5 | 2 |
3 | 4 | 1 | 1 |
Orthogonal test is tested, orthogonal test table and result of the test:
Semen Armeniacae Amarum extracts test card and result
Amount of alcohol (doubly) A | Extraction time (hour) B | Extraction time C | Amygdaloside % | |
1 | 1 | 1 | 1 | 0.1213 |
2 | 1 | 2 | 2 | 0.1092 |
3 | 1 | 3 | 3 | 0.034 |
4 | 2 | 1 | 2 | 0.1574 |
5 | 2 | 2 | 3 | 0.3442 |
6 | 2 | 3 | 1 | 0.3875 |
7 | 3 | 1 | 3 | 0.0476 |
8 | 3 | 2 | 1 | 0.2842 |
9 | 3 | 3 | 2 | 0.5442 |
Source of variation | Sum of sguares of deviation from mean | Degree of freedom | Variance | The F value | P |
A | 0.1432 | 2 | 0.07161 | 2.8888 | >0.05 |
B | 0.02057 | 2 | 0.01028 | 0.4148 | >0.05 |
C | 0.00155 | 2 | 0.000755 | 0.03128 | >0.05 |
According to Orthogonal experiment results, 4 times of amount alcohol reflux secondaries, each 1 hour, amygdaloside % content was the highest.
Following embodiment all can realize the described effect of above-mentioned experimental example
The specific embodiment
Embodiment 1:
Herba Ephedrae 90g Semen Armeniacae Amarum (stir-fry) 120g Gypsum Fibrosum 270g Radix Glycyrrhizae 90g
Radix Scutellariae 180g Radix Isatidis 180g Rhizoma Menispermi 90g
Step 1: Gypsum Fibrosum, Radix Glycyrrhizae, Rhizoma Menispermi, the Radix Isatidis five tastes, decoct secondary, add 8 times of water gagings the first time and decocted 2 hours, add 6 times of water gagings for the second time and decocted 1 hour, collecting decoction filters, and filtrate decompression is concentrated into relative density 1.05~1.06, gets extractum A;
Step 2: Herba Ephedrae decocts with water 2 times, measures decoction 2.5 hours for 8 times for the first time, and 6 times of amounts decocted 1 hour for the second time, and filtrate is concentrated into relative density 1.05~1.06, gets extractum B;
Step 3: Semen Armeniacae Amarum is with 70% alcohol reflux secondary, and each 1 hour 4 times of amount merges backflow, reclaims ethanol to there not being the alcohol flavor, extractum C;
Step 4: Radix Scutellariae decocts with water 2 times, heats water to 80 ℃ earlier for the first time, drops into Radix Scutellariae then, and twice decoction liquor filters to merge and be concentrated into relative density 1.05~1.06 filtrates, gets extractum D;
Step 5: merge extractum A, B, C, D, add Mel 200g, sucrose 100g and sodium benzoate 3g add water to total amount 1000ml, stir centrifugal behind the 50min, fill, sterilization, promptly.
Embodiment 2:
Herba Ephedrae 80g Semen Armeniacae Amarum (stir-fry) 110g Gypsum Fibrosum 290g Radix Glycyrrhizae 100g
Radix Scutellariae 170g Radix Isatidis 190g Rhizoma Menispermi 80g
Step 1: Gypsum Fibrosum, Radix Glycyrrhizae, Rhizoma Menispermi, the Radix Isatidis five tastes, decoct secondary, add 8 times of water gagings the first time and decocted 2 hours, add 6 times of water gagings for the second time and decocted 1 hour, collecting decoction filters, and filtrate decompression is concentrated into relative density 1.05~1.06, gets extractum A;
Step 2: Herba Ephedrae decocts with water 2 times, measures decoction 2.5 hours for 8 times for the first time, and 6 times of amounts decocted 1 hour for the second time, and filtrate is concentrated into relative density 1.05~1.06, gets extractum B;
Step 3: Semen Armeniacae Amarum is with 70% alcohol reflux secondary, and each 1 hour 4 times of amount merges backflow, reclaims ethanol to there not being the alcohol flavor, extractum C;
Step 4: Radix Scutellariae decocts with water 2 times, heats water to 80 ℃ earlier for the first time, drops into Radix Scutellariae then, and twice decoction liquor filters to merge and be concentrated into relative density 1.05~1.06 filtrates, gets extractum D;
Step 5: merge extractum A, B, C, D, add Mel 200g, sucrose 100g and sodium benzoate 3g add water to total amount 1000ml, stir centrifugal behind the 50min, fill, sterilization, promptly.
Embodiment 3:
Herba Ephedrae 90g Semen Armeniacae Amarum (stir-fry) 120g Gypsum Fibrosum 270g Radix Glycyrrhizae 90g
Radix Scutellariae 180g Radix Isatidis 180g Rhizoma Menispermi 90g
Step 1: Gypsum Fibrosum, Radix Glycyrrhizae, Rhizoma Menispermi, the Radix Isatidis five tastes, decoct secondary, add 8 times of water gagings the first time and decocted 2 hours, add 6 times of water gagings for the second time and decocted 1 hour, collecting decoction filters, and filtrate decompression is concentrated into relative density 1.05~1.06, gets extractum A;
Step 2: Herba Ephedrae decocts with water 2 times, measures decoction 2.5 hours for 8 times for the first time, and 6 times of amounts decocted 1 hour for the second time, and filtrate is concentrated into relative density 1.05~1.06, gets extractum B;
Step 3: Semen Armeniacae Amarum is with 70% alcohol reflux secondary, and each 1 hour 4 times of amount merges backflow, reclaims ethanol to there not being the alcohol flavor, extractum C;
Step 4: Radix Scutellariae decocts with water 2 times, heats water to 80 ℃ earlier for the first time, drops into Radix Scutellariae then, and twice decoction liquor filters to merge and be concentrated into relative density 1.05~1.06 filtrates, gets extractum D;
Step 5: merge extractum A, B, C, D, be condensed into the thick paste shape, add sucrose, dextrin is an amount of, mixing is made granule, and drying is pulverized, and sieves, and puts coldly, makes granule.
Embodiment 4:
Herba Ephedrae 90g Semen Armeniacae Amarum (stir-fry) 120g Gypsum Fibrosum 270g Radix Glycyrrhizae 90g
Radix Scutellariae 180g Radix Isatidis 180g Rhizoma Menispermi 90g
Step 1: Gypsum Fibrosum, Radix Glycyrrhizae, Rhizoma Menispermi, the Radix Isatidis five tastes, decoct secondary, add 7 times of water gagings the first time and decocted 1.5 hours, add 5 times of water gagings for the second time and decocted 1 hour, collecting decoction filters, and filtrate decompression is concentrated into relative density 1.05~1.06, gets extractum A;
Step 2: Herba Ephedrae decocts with water 2 times, measures decoction 2.5 hours for 8 times for the first time, and 6 times of amounts decocted 1 hour for the second time, and filtrate is concentrated into relative density 1.05~1.06, gets extractum B;
Step 3: Semen Armeniacae Amarum is with 70% alcohol reflux secondary, and each 1 hour 5 times of amount merges backflow, reclaims ethanol to there not being the alcohol flavor, extractum C;
Step 4: Radix Scutellariae decocts with water 2 times, heats water to 80 ℃ earlier for the first time, drops into Radix Scutellariae then, and twice decoction liquor filters to merge and be concentrated into relative density 1.05~1.06 filtrates, gets extractum D;
Step 5: merge extractum A, B, C, D, add Mel 200g, sucrose 100g and sodium benzoate 3g add water to total amount 1000ml, stir centrifugal behind the 50min, fill, sterilization, promptly.
Claims (6)
1. preparation method for the treatment of the Chinese medicine composition of infantile common cold is characterized in that comprising the steps: to choose following crude drug:
Herba Ephedrae 70-120g Semen Armeniacae Amarum (stir-fry) 100-160g Gypsum Fibrosum 220-350g
Radix Glycyrrhizae 60-120g Radix Scutellariae 150-220g Radix Isatidis 150-220g
Rhizoma Menispermi 60-120g;
Step 1: Gypsum Fibrosum, Radix Glycyrrhizae, Rhizoma Menispermi, Radix Isatidis, decoct 1-4 time, add 4-8 times of water gaging at every turn and decocted 1-3 hour, collecting decoction filters, filtrate concentrate extractum A;
Step 2: Herba Ephedrae decocts with water 1-4 time, adds 4-8 times of water gaging at every turn and decocts 1-3 hour, and collecting decoction filters, and gets extractum B;
Step 3: Semen Armeniacae Amarum is with 70% alcohol reflux 1-3 time, adds 4-8 at every turn and doubly measures 70% ethanol and decocted 0.5-3 hour, merges backflow, reclaims ethanol to there not being the alcohol flavor, extractum C;
Step 4: Radix Scutellariae decocts with water 1-3 time, heats water to 80 ℃ earlier for the first time, drops into Radix Scutellariae then, and decoction liquor filters to merge and concentrates, and gets extractum D;
Step 5: merge extractum A, B, C, D, make various dosage forms through conventional technology.
2. preparation method as claimed in claim 1 is characterized in that preparation process routinely makes capsule, tablet, granule, gel or oral liquid.
3. preparation method as claimed in claim 1 is characterized in that this method comprises the steps: to choose following crude drug:
Herba Ephedrae 90g Semen Armeniacae Amarum (stir-fry) 120g Gypsum Fibrosum 270g Radix Glycyrrhizae 90g
Radix Scutellariae 180g Radix Isatidis 180g Rhizoma Menispermi 90g
Step 1: Gypsum Fibrosum, Radix Glycyrrhizae, Rhizoma Menispermi, the Radix Isatidis five tastes, decoct secondary, add 8 times of water gagings the first time and decocted 2 hours, add 6 times of water gagings for the second time and decocted 1 hour, collecting decoction filters, and filtrate decompression is concentrated into relative density 1.05~1.06, gets extractum A;
Step 2: Herba Ephedrae decocts with water 2 times, measures decoction 2.5 hours for 8 times for the first time, and 6 times of amounts decocted 1 hour for the second time, and filtrate is concentrated into relative density 1.05~1.06, gets extractum B;
Step 3: Semen Armeniacae Amarum extracts secondary with 70% alcohol reflux, and each 1 hour 4 times of amount merges backflow, and recovery ethanol gets extractum C to there not being the alcohol flavor;
Step 4: Radix Scutellariae decocts with water 2 times, heats water to 80 ℃ earlier for the first time, drops into Radix Scutellariae then, and twice decoction liquor filters to merge and be concentrated into relative density 1.05~1.06 filtrates, gets extractum D;
Step 5: merge extractum A, B, C, D, add Mel 200g, sucrose 100g and sodium benzoate 3g add water to total amount 1000ml, stir centrifugal behind the 50min, fill, sterilization, promptly.
4. the application of Chinese medicine composition in the golden Portugal of preparation inhibition bacterium medicine for the treatment of infantile common cold, described compositions is prepared by following method:
Choose following crude drug:
Herba Ephedrae 70-120g Semen Armeniacae Amarum (stir-fry) 100-160g Gypsum Fibrosum 220-350g
Radix Glycyrrhizae 60-120g Radix Scutellariae 150-220g Radix Isatidis 150-220g
Rhizoma Menispermi 60-120g;
Step 1: Gypsum Fibrosum, Radix Glycyrrhizae, Rhizoma Menispermi, Radix Isatidis, decoct 1-4 time, add 4-8 times of water gaging at every turn and decocted 1-3 hour, collecting decoction filters, filtrate concentrate extractum A;
Step 2: Herba Ephedrae decocts with water 1-4 time, adds 4-8 times of water gaging at every turn and decocts 1-3 hour, and collecting decoction filters, and gets extractum B;
Step 3: Semen Armeniacae Amarum extracts 1-3 time with 70% alcohol reflux, adds 4-8 at every turn and doubly measures 70% alcohol decoction 0.5-3 hour, merges backflow, and recovery ethanol gets extractum C to there not being pure flavor;
Step 4: Radix Scutellariae decocts with water 1-3 time, heats water to 80 ℃ earlier for the first time, drops into Radix Scutellariae then, and decoction liquor filters to merge and concentrates, and gets extractum D;
Step 5: merge extractum A, B, C, D, make various dosage forms through conventional technology.
5. method as claimed in claim 4, described compositions is prepared by following method: choose following crude drug:
Herba Ephedrae 90g Semen Armeniacae Amarum (stir-fry) 120g Gypsum Fibrosum 270g Radix Glycyrrhizae 90g
Radix Scutellariae 180g Radix Isatidis 180g Rhizoma Menispermi 90g
Step 1: Gypsum Fibrosum, Radix Glycyrrhizae, Rhizoma Menispermi, the Radix Isatidis five tastes, decoct secondary, add 8 times of water gagings the first time and decocted 2 hours, add 6 times of water gagings for the second time and decocted 1 hour, collecting decoction filters, and filtrate decompression is concentrated into relative density 1.05~1.06, gets extractum A;
Step 2: Herba Ephedrae decocts with water 2 times, measures decoction 2.5 hours for 8 times for the first time, and 6 times of amounts decocted 1 hour for the second time, and filtrate is concentrated into relative density 1.05~1.06, gets extractum B;
Step 3: Semen Armeniacae Amarum is with 70% alcohol reflux secondary, and each 1 hour 4 times of amount merges backflow, reclaims ethanol to there not being the alcohol flavor, extractum C;
Step 4: Radix Scutellariae decocts with water 2 times, heats water to 80 ℃ earlier for the first time, drops into Radix Scutellariae then, and twice decoction liquor filters to merge and be concentrated into relative density 1.05~1.06 filtrates, gets extractum D;
Step 5: merge extractum A, B, C, D, add Mel 200g, sucrose 100g and sodium benzoate 3g add water to total amount 1000ml, stir centrifugal behind the 50min, fill, sterilization, promptly.
6. as any described preparation method of claim 1-3, wherein the detection method of amygdaloside is in the preparation: chromatographic column is: Kromasil C18 (4.6*150mm, 5 μ m); Mobile phase is: acetonitrile-water (10: 90); The detection wavelength is 210nm.
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CN102319308A (en) * | 2011-08-26 | 2012-01-18 | 太极集团重庆涪陵制药厂有限公司 | Preparation method of heat-clearing cough-relieving oral liquid for children |
CN105214049A (en) * | 2015-10-29 | 2016-01-06 | 张结莲 | A kind of Chinese medicine composition for the treatment of infantile common cold and preparation method thereof |
CN108452111A (en) * | 2018-06-12 | 2018-08-28 | 赵翔 | A kind of Chinese medicine preparation for treating cold in children |
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CN101537056A (en) * | 2008-03-19 | 2009-09-23 | 北京亚东生物制药有限公司 | Method for preparing Chinese medicinal preparation for treating external respiration infection of children |
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CN102319308A (en) * | 2011-08-26 | 2012-01-18 | 太极集团重庆涪陵制药厂有限公司 | Preparation method of heat-clearing cough-relieving oral liquid for children |
CN102319308B (en) * | 2011-08-26 | 2013-04-17 | 太极集团重庆涪陵制药厂有限公司 | Preparation method of heat-clearing cough-relieving oral liquid for children |
CN105214049A (en) * | 2015-10-29 | 2016-01-06 | 张结莲 | A kind of Chinese medicine composition for the treatment of infantile common cold and preparation method thereof |
CN108452111A (en) * | 2018-06-12 | 2018-08-28 | 赵翔 | A kind of Chinese medicine preparation for treating cold in children |
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