CN102120112A - Cell filtering device - Google Patents

Abstract

A cell filtering device, the filtering device includes a filter cartridge (1), the opening end of the filter cartridge (1) is provided with a brim (2), and the other end is tapered; the filter cartridge (1) is composed of an annular The brim inner ring (3), the support rod (4) fixed on the brim inner ring (3) and the filter membrane (5) glued on the support rod (4) are formed. The invention has the advantages of simple structure and convenient use, and the brim design of the brim enables the filter device to be effectively positioned on different liquid-contacting containers for experimental operation. By directly injecting the cell suspension or mixture into the filter device through the opening, the cells are filtered by gravity, and the operation is convenient.

Description

a cell filter

technical field

The invention relates to a filtering device, in particular to a cell filtering device.

Background technique

At present, in biomedical research, cells are often used as models for in vitro research. In the research process, the screening and separation of cells are often involved. Especially when some instruments are used for detection, in order to ensure the accuracy of the detection and the practical safety of the instrument, it is necessary to filter the cells to be detected. When using traditional cell filtration methods and devices, it is necessary to manually cut the filter membrane, fold the filter membrane into a funnel shape, and apply it to the filter device. The filtration operation process using this device often requires the cooperation of multiple people, which brings great inconvenience to the experiment. However, the filters currently on the market are relatively large and cannot be effectively combined with other instruments in the experimental process. Therefore, it is extremely inconvenient to use, which not only wastes time and manpower, but also easily causes experimental errors.

Contents of the invention

In order to overcome the deficiencies in the prior art referred to above, the present invention provides a cell filtration device with a simple structure, easy to use, and a support frame and a filter membrane firmly glued together.

The present invention is achieved through the following technical solutions: a cell filtration device, the filtration device includes a filter cartridge, the opening end of the filter cartridge is provided with a brim, and the other end is tapered; the filter cartridge is composed of an annular brim The inner ring is composed of a support rod fixed at one end on the brim inner ring and a filter membrane glued on the support rod. The filter membrane is attached to the brim inner ring. The bottom of the filter membrane is conical.

Further, the filter membrane is a polyamide fiber microporous filter membrane.

Further, the brim and the support rod are made of stainless steel or plastic materials.

The invention has simple structure and is convenient to use, and the brim design of the brim enables the filter device to be effectively positioned on different liquid-contacting containers for filtering operations. The operator injects the cell suspension or mixture directly from the opening end of the filter device, uses gravity to filter cells, and filters cells of different sizes through filter membranes with different pore sizes, which is convenient to operate.

Description of drawings

Accompanying drawing 1 is a structural representation of the present invention;

Accompanying drawing 2 is the top view of the present invention.

In the figure: 1-filter cartridge, 2-brim, 3-brim inner ring, 4-support rod, 5-filter membrane.

Detailed ways

In order to facilitate the understanding of those skilled in the art, the present invention will be further described below in conjunction with the accompanying drawings.

A kind of cell filtering device, as shown in accompanying drawing 1, 2, described filtering device comprises filter cartridge 1, and the open end of described filter cartridge 1 is provided with brim 2, and the other end is conical; This filter cartridge 1 is formed by annular The brim inner ring 3 of the hat brim, one end of which is affixed to a number of support rods 4 on the brim inner ring 3 and the filter membrane 5 glued on the support rod 4 to form. The bottom of the filter membrane 5 is conical. The filter membrane 5 is attached and connected to the brim inner ring 3 . The plurality of support rods 4 are arranged along the brim inner ring 3 .

Membrane holes are evenly distributed on the filter membrane 5, and the diameter of the membrane holes is 5-10 microns. The filter membrane 5 is a polyamide fiber microporous filter membrane.

The brim 2 is made of stainless steel or plastic material. By designing the size of the brim 2, the filter device is suitable for different liquid-contacting containers, so that it can be effectively positioned on the containers involved in the operation process to perform filtering operations.

The support rod 4 is made of stainless steel or plastic material to fix the filter membrane, and is fixed with the filter membrane 5 by using specific glue.

When utilizing the present invention to carry out cell filtration operation, the filter device can be placed in the liquid contact container, the brim 2 is placed on the container mouth for positioning, and the cell suspension or mixture is directly injected into the filter device at a constant speed from the open end, and through the action of gravity Filter the cells. The filter device can be used to filter cells of different sizes by gluing filter membranes of different pore sizes.

The present invention can be used for cell washing, filtration and enrichment, etc., by adding liquid samples into the filter device, the cells to be collected in the liquid will stay on the filter membrane, while other liquids will flow away after passing through the filter membrane, thereby realizing The purpose of cell enrichment to be collected.

The contents mentioned in the above embodiments are not intended to limit the present invention, and any obvious replacements are within the protection scope of the present invention without departing from the concept of the present invention.

Claims (6)

1. cell filtration device, described filter comprises filter cylinder (1), it is characterized in that: the openend of described filter cylinder (1) is provided with shade (2), and the other end is tapered; This filter cylinder (1) is fixed in the filter membrane (5) that encircles the support bar (4) on (3) in the shade and be glued on the support bar (4) and is constituted by ring (3) in the shade of annular.

2. cell filtration device according to claim 1 is characterized in that: described filter membrane (5) bottom is conical.

3. cell filtration device according to claim 2 is characterized in that: described filter membrane (5) is fitted and connected ring (3) in shade.

4. cell filtration device according to claim 3 is characterized in that: described support bar (4) one ends are fixed in the shade and encircle on (3).

5. according to each described cell filtration device among the claim 1-4, it is characterized in that: described filter membrane is the Fypro miillpore filter.

6. according to each described cell filtration device among the claim 1-4, it is characterized in that: described shade and support bar adopt stainless steel or plastic material.

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