CN102113624B - Selenium yeast for feeds and preparation method thereof - Google Patents
Selenium yeast for feeds and preparation method thereof Download PDFInfo
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Abstract
The invention relates to selenium yeast for feeds and a preparation method thereof. The preparation method of the selenium yeast for feeds comprises the following steps of: mixing beer yeast subjected to secondary liquid culture with a feed fermentation substrate and water in a weight ratio of (1-2) to (10-20) to (5-10); fermenting at 30-45 DEG C for 36-78h; and then, drying and crushing the fermented materials to obtain a finished selenium yeast product for feeds in the invention. The method provided by the invention is simple and easy to produce in batch. The selenium yeast prepared by utilizing the method of the invention contains organic selenium up to 500-1000 mg/kg, and the selenium product provided by the invention contains live yeast bacteria up to 2 billion/g and crude protein up to 35 percent, is safe and non-toxic and has less investment, rapid effect and high benefits.
Description
Technical field
The present invention relates to a kind of feed with selenium yeast and preparation method thereof; Particularly about a kind of barms and inorganic selenium source of selecting high-quality for use; Adopt liquid, solid-state combined ferment technology to prepare the method for feed, and use selenium yeast according to the prepared feed that obtains of this method with selenium yeast.
Background technology
Trace elements of selenium has effects such as participating in paddy Guang liver peptide peroxidase makes the humans and animals body avoid the destruction of free radical and radiation, keeps cellular respiration, immune response stimulating, protection liver, inhibition cancer cell.In the production of traditional feed and feed addictive, being widely used, inorganic selenium---sodium selenite is as the replenishers of trace elements of selenium.But the toxicity of sodium selenite is big, uses or miscarriage, can cause serious adverse consequences, thereby developed countries such as America and Europe, Japan does not re-use this type of inorganic selenium source in animal feed.
Research shows that the bioavailability of organic selenium, particularly selenium yeast is high, can improve the blood selenium level in the animal body effectively, can be used as feed addictive and be used for the ruminant animal, and be a kind of selenium supplement that market prospects are arranged very much.Selenium yeast is a kind of biologic product; Brewer's yeast, candida utili have that growth and breeding is fast, fermentation period is short, to characteristics such as micro-absorptivity height; It is the ideal carrier that inorganic selenium is converted into organic selenium; Inorganic selenium forms the organic selenium (selenomethionine, selenium cystine) in the similar wholefood with amino acid or protein bound after getting into yeast cells, and wherein organic selenium content accounts for more than 90% of total selenium; And can ignore inorganic selenium content in the selenium yeast, and its active and absorptivity are 10～20 times of inorganic selenium.Se content in the selenium yeast of the prior art is generally below 500mg/kg.
The preparation of China's selenium yeast at present also is in the laboratory research stage, and the actual production of being unrealized, correlative study also lack the exploration to the optimal conditions of rich selenium fermentation, does not possess the directive significance of large-scale production.
Summary of the invention
One object of the present invention is to provide a kind of method for preparing feed with selenium yeast, adopts simple and easy to do zymotechnique, and preparing a kind of selenium yeast of high Se content, and this method is easy to large-scale production and implements.
Another object of the present invention is to provide a kind of selenium yeast for preparing according to described method, wherein contain the organic selenium and the active dry yeasr of high level, can be used as feed or feed addictive.
On the one hand; The invention provides a kind of microbial fermentation and prepare the method for feed with selenium yeast; It wherein mainly is the barms of selecting high-quality for use; Be inoculated into earlier in the seeding tank fluid nutrient medium that adds sodium selenite and carry out enlarged culture, and then be inoculated in the solid-state tray and ferment, obtain feed of the present invention after the drying and use selenium yeast.
According to specific embodiments of the present invention, microbial fermentation provided by the invention prepares feed and comprises with the method for selenium yeast:
The ratio of brewer's yeast in every ring 40～80ml is inoculated in the brewer's wort enriched medium (or claiming the one-level enriched medium), and 28～32 ℃ of vibrations were carried out one-level in 22～72 hours preferred 22～32 hours and are increased the bacterium cultivation;
Secondary seed medium (or claiming the secondary enriched medium) is heated to 55 ℃～65 ℃; (addition of AMS is decided according to the content of starch in the culture medium to add AMS; The amylase that every gram starch adding 6～10U enzyme is lived) the insulation hydrolysis is 2～3 hours; Add carbohydrase (addition of carbohydrase also is to decide according to the content of starch in the culture medium, and every gram starch adds the carbohydrase that 100～180U enzyme is lived) then, keep 55 ℃～65 ℃ saccharification 2～3 hours; Be warming up to 100 ℃～125 ℃ sterilizations 15～30 minutes afterwards, add selenate or selenite again and make that selenium concentration reaches 20～25 μ g/ml in this culture medium; And this nutrient solution is cooled to below 35 ℃, the ratio in 8%～12% inserts and above-mentionedly increases the saccharomyces cerevisiae liquid after bacterium is cultivated through one-level, carries out secondary in 22～48 hours in 28 ℃～32 ℃ shaken cultivation of temperature and increases bacterium and cultivate;
Increase bacterium liquid that bacterium cultivates and feed fermentation substrate and water according to 1～2 with above-mentioned through secondary: 10～20: 5～10 weight ratio mixing; 30～45 ℃ of condition bottom fermentations 36～78 hours; With the drying materials after the fermentation ends, pulverizing, promptly get feed and use the selenium yeast finished product.
According to preferred specific embodiments of the present invention, in the method for the present invention, said brewer's wort enriched medium is processed by following method:
Brewer's wort adds the distilled water of 6～10 times of volumes, sugar degree regulation to 4～10 ripple woodss (Balling), and pH transfers to 5.5～6.5, under 100～125 ℃ of temperature, sterilizes 15～30 minutes.
According to preferred specific embodiments of the present invention, in the method for the present invention, said secondary seed medium adds water by corn flour, wheat bran, nutritive salt and is mixed and made into, and specifically processes by following method:
Corn flour 15～20 weight portions, wheat bran 3～8 weight portions, nutritive salt 1～2 weight portion add water 70～81 weight portions, mix, and pH transfers to 3.6～4.8 and processes.
According to preferred specific embodiments of the present invention; In the method for the present invention; Said feed fermentation substrate (solid fermentation substrate) is processed after adding the water mixing by corn flour, cotton dregs, dregs of beans, the rape dregs of rice, wheat bran, nutritive salt, interpolation selenate or selenite, specifically processes by following method:
Corn flour 50～70 weight portions, cotton dregs 8～12 weight portions, dregs of beans 8～12 weight portions, the rape dregs of rice 4～8 weight portions, wheat bran 5～10 weight portions, nutritive salt 1～2 weight portion, selenate or selenite 0.5～1 weight portion mix, and process the feed fermentation substrate.
According to preferred specific embodiments of the present invention, in the method for the present invention, said nutritive salt be by potassium dihydrogen phosphate, ammonium sulfate, glucose according to 2～4: 8～12: 1～3 weight ratio mixes to be processed.
According to preferred specific embodiments of the present invention; In the method for the present invention; Saidly increase in the bacterium liquid and feed fermentation substrate and water blend step that bacterium cultivates through secondary, the pH value of described water is 5.0～7.0, and the granularity of said feed fermentation substrate is 3～8mm; Mix mixture to be piled the trapezoidal heap that height is 10～40cm after finishing, 30～45 ℃ of condition bottom fermentations 36～78 hours.
According to preferred specific embodiments of the present invention, in the method for the present invention, in the said baking step, the control bake out temperature is not higher than 60 ℃, preferably is not higher than 45 ℃.
According to preferred specific embodiments of the present invention, in the method for the present invention, the material grinding granularity after the said oven dry is passed through 20 mesh sieves through 80 mesh sieves, at least 95% through 40 mesh sieves, 100% at least 75%.
In of the present invention one preferred specific embodiments, microbial fermentation of the present invention prepares feed to carry out according to following method with the method for selenium yeast:
Brewer's yeast on the test tube slant is inoculated in the ratio of every ring 40～80ml the brewer's wort enriched medium (distilled water that is added 6～10 times of volumes by brewer's wort is housed; Sugar degree regulation to 4～10 ripple woodss; PH transfers to 5.5～6.5, under 100～125 ℃ of temperature, sterilizes and processes in 15～30 minutes) triangular flask in, bottle stopper beyond the Great Wall; And wrap with sealing film, under 28～32 ℃, 100～180 rev/mins conditions, vibrate and carried out one-level in 22～72 hours and increase bacterium and cultivate;
(adding water 70～81 weight portions by corn flour 15～20 weight portions, wheat bran 3～8 weight portions, nutritive salt 1～2 weight portion mixes with secondary seed medium; PH transfers to 3.6～4.8 and processes) be heated to 55 ℃～65 ℃; Add an amount of AMS (adding the amylase that 6～10U enzyme is lived) insulation hydrolysis 2～3 hours according to every gram starch in the culture medium; Add an amount of carbohydrase (adding the carbohydrase that the preferred 120～160U enzyme of 100～180U is lived) then according to every gram starch in the culture medium; Keep 55 ℃～65 ℃ saccharification 2～3 hours; Be warming up to 100 ℃～125 ℃ sterilizations 15～30 minutes afterwards, cool to again below 50 ℃, sodium selenite solution is added in this culture medium by a certain percentage; Make that selenium concentration reaches 20～25 μ g/ml in this culture medium; Nutrient solution continues to be cooled to below 35 ℃, increases saccharomyces cerevisiae liquid that bacterium cultivates by 8%～12% mass ratio inoculation with above-mentioned through one-level, is that stir culture was carried out secondary in 22～48 hours and increased bacterium and cultivate under 28 ℃～32 ℃, 100～180 rev/mins conditions in temperature;
Increase bacterium liquid that bacterium cultivates and water (in advance with inorganic acid or organic acid for example adjusting such as HCl, citric acid pH to 5.0～7.0), feed fermentation substrate through secondary and (mix by corn flour 50～70 weight portions, cotton dregs 8～12 weight portions, dregs of beans 8～12 weight portions, the rape dregs of rice 4～8 weight portions, wheat bran 5～10 weight portions, nutritive salt 1～2 weight portion, selenate or selenite 0.5～1 weight portion and to process above-mentioned; Granularity is 3～8mm) with 1～2: 5～10: 10～20 weight ratio is evenly mixed; After mixing finishes it is piled the trapezoidal heap that height is the preferred 10～30cm of 10～40cm; Simultaneously with floor heating 30～45 ℃ of condition bottom fermentations 36～78 hours; Tumble Dry after the fermentation ends; For reducing the destruction of bake out temperature, in the said baking step, preferably control temperature and be not higher than 60 ℃ active component in the feed; More preferably below 40 ℃; Material after will drying is afterwards pulverized, and preferred grinding particle size passes through 20 mesh sieves through 80 mesh sieves, at least 95% through 40 mesh sieves, 100% at least 75%, and pulverizing the back packing is finished product.
The present invention also provides the feed for preparing according to the method described above to use selenium yeast.This feed is with in the selenium yeast, organic selenium content 600～1000 micrograms/g, and the yeast viable count is up to more than 1,800,000,000/g, and crude protein content is up to more than 32%.Feed of the present invention is used selenium yeast, can be used as feed or feed addictive, be used for feeding animals particularly ruminant with the Selenium Supplement element.Related experiment shows, utilizes selenium yeast feeding animals of the present invention can increase the content of ruminant Ruzhong selenium; Reduce SCC number in the newborn body; Improve the calf survival rate; Reduce the breeding number of times of each gestation; Reduce disease incidence rate such as retention of afterbirth, hysteritis; Improve food conversion ratio, reduce the death rate, improve body disease-resistant power and immunity, accelerate the reaction of body vaccine.
In sum; The present invention selects the barms of function admirable for use; And adopt liquid, the fixed zymotechnique that closes; After postvaccinal culture medium being carried out degree of depth fermentation, can obtain a large amount of Se-enriched yeast products, thereby provide a kind of preparation method who is easy to the selenium yeast of large-scale production to reach according to the prepared selenium yeast that obtains of this method.Selenium yeast of the present invention and preparation method thereof has with advantage:
1, method of the present invention adopts liquid, the fixed zymotechnique that closes, and after postvaccinal culture medium being carried out degree of depth fermentation, obtains a large amount of Se-enriched yeast products, and the conversion ratio that adopts this technology can effectively improve selenium is avoided the generation of simple substance selenium.Solid state fermentation is produced selenium yeast with respect to pure liquid state fermentation simultaneously, and the simple cost of technology is lower, and less equipment investment can be carried out large-scale production.
2, the present invention does not have specific (special) requirements to Zymolysis Equipment and condition, and the equipment and the condition of general fermentation plant all can be produced, and the production investment is less, and method is simple, and is with low cost, practical, easy and simple to handle, is easy to generally promote.
3, organic selenium content is higher in the selenium yeast product of the present invention, and quality is good, and its organic selenium content contains 500～1000 micrograms up to every gram fermentate, contains yeast viable count 2,000,000,000/g, crude protein content 35% in the product of the present invention simultaneously.This selenium yeast product safety is nontoxic, small investment, instant effect, high efficiency.
Description of drawings
Fig. 1 prepares the schematic flow sheet of selenium yeast for microbial fermentation of the present invention.
The specific embodiment
Below specify technology of the present invention and characteristics through specific embodiment, but these embodiment are not in order to limit protection scope of the present invention.
The used bacterial classification of embodiment: used brewer's yeast (Saccharemycescerevisiae) bacterial classification is bought in China Committee for Culture Collection of Microorganisms common micro-organisms center in following examples of the present invention.Preserving number is: CGMCC AS2.2.
Embodiment 1
Prepare the selenium yeast of present embodiment according to following method, can be simultaneously referring to flow process shown in Figure 1:
One-level increases bacterium and cultivates: the brewer's yeast picking five rings on the test tube slant is seeded in 300ml brewer's wort enriched medium is housed (said brewer's wort enriched medium adds 1000ml distilled water by brewer's wort 130g; Sugar degree regulation to 8 ripple woods, pH transfers to 6.0, and sterilization was processed in 20 minutes under 121 ℃ of temperature; Measure 300ml) triangular flask in (triangular flask capacity 500ml); Bottle stopper beyond the Great Wall, and wrap with sealing film, vibration was carried out one-level in 24 hours and is increased bacterium and cultivate under 30 ℃, 120 rev/mins conditions;
Secondary increases bacterium and cultivates: the 50kg secondary seed medium (is added water 39.25 weight portions and mixed by corn flour 8 weight portions, wheat bran 2 weight portions, nutritive salt (mixed according to 1.5: 5: 1 process by potassium dihydrogen phosphate, ammonium sulfate, glucose) 0.75 weight portion; PH transfers to 4.0; 121 ℃ of sterilizations were processed in 30 minutes) be heated to 60 ℃; Add 8U enzyme AMS insulation hydrolysis alive 3 hours according to every gram starch in the culture medium; Add the carbohydrase that the 140U enzyme is lived according to every gram starch in the culture medium then, keep 60 ℃ of saccharification 2 hours, be warming up to 120 ℃ of sterilizations 30 minutes afterwards; Cool to again below 50 ℃; (Se content 43.806mg/ml) adds in this culture medium by a certain percentage with sodium selenite solution, makes that selenium concentration reaches 25 μ g/ml in this culture medium, and nutrient solution continues to be cooled to below 35 ℃; Increasing the saccharomyces cerevisiae liquid 5kg that bacterium cultivates through one-level and be inoculated in this secondary medium above-mentioned, is that stir culture was carried out secondary in 24 hours and increased bacterium and cultivate under 30 ℃, the 130 rev/mins conditions in temperature;
Solid state fermentation: with above-mentioned through secondary increase bacterium liquid 50kg that bacterium cultivates and water (in advance with inorganic acid or organic acid for example adjusting such as HCl, citric acid pH to 6.0) 250kg, (corn flour 325kg, cotton dregs 50kg, dregs of beans 50kg, rape dregs of rice 30kg, wheat bran 35kg, nutritive salt 7.5kg (mixed and processed by potassium dihydrogen phosphate 1.5kg, ammonium sulfate 5kg, glucose 1kg), selenite 2.5kg mix and process the feed fermentation substrate; Granularity be 3～8mm) 500kg with 1～2: 5～10: 10～20 weight ratio is evenly mixed; Piling it high after mixing finishes is the trapezoidal heap of 20cm; Simultaneously with floor heating 30 ℃ of condition bottom fermentations 72 hours; Tumble Dry after the fermentation ends; For reducing the destruction of bake out temperature to active component in the feed, the control temperature is about 45 ℃ in the said baking step, and the material after will drying is afterwards pulverized; Preferred grinding particle size passes through 20 mesh sieves through 80 mesh sieves, at least 95% through 40 mesh sieves, 100% at least 75%, and pulverizing the back packing is finished product.
The present invention also provides the feed for preparing according to the method described above to use selenium yeast.This feed is with in the selenium yeast, organic selenium content 777 μ g/g, and the yeast viable count is up to 2,000,000,000/g, and crude protein content is up to 35%.
Embodiment 2
The selenium yeast for preparing present embodiment according to following method:
One-level increases bacterium and cultivates: the brewer's yeast on the test tube slant is inoculated in the ratio of the about 60ml of every ring the brewer's wort enriched medium (distilled water that is added 7.5 times of volumes by brewer's wort is housed; Sugar degree regulation to 6 ripple woods; PH transfers to 6.5, and sterilization was processed in 25 minutes under 120 ℃ of temperature) triangular flask in, bottle stopper beyond the Great Wall; And wrap with sealing film, vibration was carried out one-level in 24 hours and is increased bacterium and cultivate under 30 ℃, 120 rev/mins conditions;
Secondary increases bacterium and cultivates: the 100kg secondary seed medium (is added water 76.5 weight portions and mixed by corn flour 18 weight portions, wheat bran 4 weight portions, nutritive salt (mixed according to 1.5: 5: 1 process by potassium dihydrogen phosphate, ammonium sulfate, glucose) 1.5 weight portions; PH transfers to 4.5; 121 ℃ of sterilizations were processed in 30 minutes) be heated to 60 ℃, add 8U enzyme AMS insulation hydrolysis alive 2.5 hours according to every gram starch in the culture medium, add the carbohydrase that the 140U enzyme is lived according to every gram starch in the culture medium then; Keep 60 ℃ of saccharification 2.5 hours; Be warming up to 121 ℃ of sterilizations 20 minutes afterwards, cooling again adds sodium selenite solution (Se content 43.806mg/ml); Make that selenium concentration reaches 20 μ g/ml in this culture medium; Nutrient solution continues to be cooled to below 35 ℃, and increase the saccharomyces cerevisiae liquid 10kg that bacterium cultivates through one-level and be inoculated in this secondary medium above-mentioned, be that stir culture was carried out secondary in 24 hours and increased bacterium and cultivate under 30 ℃, the 150 rev/mins conditions in temperature;
Solid state fermentation: with above-mentioned through secondary increase bacterium liquid 100kg that bacterium cultivates and water 600kg (in advance with inorganic acid or organic acid for example adjusting such as HCl, citric acid pH to 6.5), feed fermentation substrate 1000kg (is mixed and is processed by corn flour 60 weight portions, cotton dregs 10 weight portions, dregs of beans 10 weight portions, the rape dregs of rice 6 weight portions, wheat bran 7 weight portions, nutritive salt 1.5 weight portions, selenite 0.75 weight portion; Sterilized 30 minutes for 121 ℃; Granularity is 3～8mm) evenly mixing; Piling it high after mixing finishes is the trapezoidal heap of 30cm; Simultaneously with floor heating 45 ℃ of condition bottom fermentations 48 hours; Tumble Dry after the fermentation ends, 50 ℃ of bake out temperatures, the material after will drying is afterwards pulverized; Preferred grinding particle size passes through 20 mesh sieves through 80 mesh sieves, at least 95% through 40 mesh sieves, 100% at least 75%, and pulverizing back packing promptly gets the feed of present embodiment and uses the selenium yeast finished product.
The feed that present embodiment makes is used selenium yeast.This feed is with in the selenium yeast, organic selenium content 682 micrograms/g, and more than yeast viable count 18.5 hundred million/g, crude protein content is up to 33.8%.
Embodiment 3
The selenium yeast for preparing present embodiment according to following method:
One-level increases bacterium and cultivates: the brewer's yeast on the test tube slant is inoculated in the ratio of every ring 50ml the brewer's wort enriched medium (distilled water that is added 8 times of volumes by brewer's wort is housed; Sugar degree regulation to 10 ripple woods; PH transfers to 6.0, and sterilization was processed in 20 minutes under 121 ℃ of temperature) triangular flask in, bottle stopper beyond the Great Wall; And wrap with sealing film, vibration was carried out one-level in 28 hours and is increased bacterium and cultivate under 30 ℃, 150 rev/mins conditions;
Secondary increases bacterium and cultivates: the 50kg secondary seed medium (is added water 78.5 weight portions and mixed by corn flour 15 weight portions, wheat bran 5 weight portions, nutritive salt (mixed according to 1.5: 5: 1 process by potassium dihydrogen phosphate, ammonium sulfate, glucose) 1.5 weight portions; PH transfers to 4.0; 121 ℃ of sterilizations were processed in 20 minutes) be heated to 55 ℃, add 8U enzyme AMS insulation hydrolysis alive 2.5 hours according to every gram starch in the culture medium, add the carbohydrase that the 140U enzyme is lived according to every gram starch in the culture medium then; Keep about 2 hours of 55 ℃ of saccharification; Be warming up to 121 ℃ of sterilizations 20 minutes afterwards, cooling again adds sodium selenite solution (Se content 43.806mg/ml); Make that selenium concentration reaches 25 μ g/ml in this culture medium; Nutrient solution continues to be cooled to below 35 ℃, and increase the about 5kg of saccharomyces cerevisiae liquid that bacterium cultivates through one-level and be inoculated in this secondary medium above-mentioned, be that stir culture was carried out secondary in 24 hours and increased the bacterium cultivation under 30 ℃, the 150 rev/mins conditions in temperature;
Solid state fermentation: with above-mentioned through secondary increase the about 55kg of bacterium liquid that bacterium cultivates and water 330kg (in advance with inorganic acid or organic acid for example adjusting such as HCl, citric acid pH to 6.0), feed fermentation substrate 820kg (is mixed and is processed by corn flour 60 weight portions, cotton dregs 10 weight portions, dregs of beans 8 weight portions, the rape dregs of rice 6 weight portions, wheat bran 8 weight portions, nutritive salt 1.2 weight portions, selenite 0.8 weight portion; Granularity is 3～8mm; Sterilized 30 minutes for 121 ℃) evenly mix; Piling it high after mixing finishes is the trapezoidal heap of 20cm; Simultaneously with floor heating 40 ℃ of condition bottom fermentations 72 hours; Tumble Dry after the fermentation ends, 40 ℃ of bake out temperatures, the material after will drying is afterwards pulverized; Preferred grinding particle size passes through 20 mesh sieves through 80 mesh sieves, at least 95% through 40 mesh sieves, 100% at least 75%, and pulverizing back packing promptly gets the feed of present embodiment and uses the selenium yeast finished product.
The feed that present embodiment makes is used selenium yeast.This feed is with in the selenium yeast, organic selenium content 880 micrograms/g, and the yeast viable count is up to more than about 23.2 hundred million/g, and crude protein content is up to 37.6%.
Claims (8)
1. a feed is with the preparation method of selenium yeast, and this method comprises:
The ratio of brewer's yeast in every ring 40～80ml is inoculated in the brewer's wort enriched medium, and 28～32 ℃ of vibrations were carried out one-level in 22～72 hours and are increased the bacterium cultivation;
Secondary seed medium is heated to 55 ℃～65 ℃; Add AMS insulation hydrolysis 2～3 hours; Add carbohydrase then; Keep 55 ℃～65 ℃ saccharification 2～3 hours, be warming up to 100 ℃～125 ℃ sterilizations 15～30 minutes afterwards, add selenate or selenite again and make that selenium concentration reaches 20～25 μ g/ml in this culture medium; And this nutrient solution is cooled to below 35 ℃, the ratio in 8%～12% inserts and above-mentionedly increases the saccharomyces cerevisiae liquid after bacterium is cultivated through one-level, carries out secondary in 22～48 hours in 28 ℃～32 ℃ shaken cultivation of temperature and increases bacterium and cultivate; Wherein, said secondary seed medium is processed by following method: corn flour 15～20 weight portions, wheat bran 3～8 weight portions, nutritive salt 1～2 weight portion add water 70～81 weight portions, mix, and pH transfers to 3.6～4.8 and processes;
Increase bacterium liquid that bacterium cultivates and feed fermentation substrate and water according to 1～2 with above-mentioned through secondary: 10～20: 5～10 weight ratio mixing; 30～45 ℃ of condition bottom fermentations 36～78 hours; With the drying materials after the fermentation ends, pulverizing, promptly get feed and use the selenium yeast finished product; Wherein, Said feed fermentation substrate is processed by following method: corn flour 50～70 weight portions, cotton dregs 8～12 weight portions, dregs of beans 8～12 weight portions, the rape dregs of rice 4～8 weight portions, wheat bran 5～10 weight portions, nutritive salt 1～2 weight portion, selenate or selenite 0.5～1 weight portion mix, and process the feed fermentation substrate.
2. method according to claim 1, wherein, said brewer's wort enriched medium is processed by following method:
Brewer's wort adds the distilled water of 6～10 times of volumes, sugar degree regulation to 4～10 ripple woodss, and pH transfers to 5.5～6.5, under 100～125 ℃ of temperature, sterilizes 15～30 minutes.
3. method according to claim 1, wherein, said nutritive salt be by potassium dihydrogen phosphate, ammonium sulfate, glucose according to 2～4: 8～12: 1～3 weight ratio mixes to be processed.
4. method according to claim 1; Wherein, Saidly increase in the bacterium liquid and feed fermentation substrate and water blend step that bacterium cultivates through secondary, the pH value of described water is 5.0～7.0, and the granularity of said feed fermentation substrate is 3～8mm; Mix mixture to be piled the trapezoidal heap that height is 10～40cm after finishing, 30～45 ℃ of condition bottom fermentations 36～78 hours.
5. method according to claim 1, wherein, in the said baking step, the control bake out temperature is not higher than 60 ℃; Material grinding granularity after the said oven dry is passed through 20 mesh sieves through 80 mesh sieves, at least 95% through 40 mesh sieves, 100% at least 75%.
6. method according to claim 1, wherein, said yeast is brewer's yeast (Saccharemyces cerevisiae) CGMCC AS2.2.
7. the feed for preparing according to each said method of claim 1～6 is used selenium yeast.
8. feed according to claim 7 is used selenium yeast, and this feed is with organic selenium content 600～1000 micrograms/g in the selenium yeast, and more than yeast viable count 1,800,000,000/g, crude protein content is more than 32%.
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| CN111631302A (en) * | 2020-05-20 | 2020-09-08 | 百色三宝生物技术有限公司 | Method for producing organic selenium by solid two-stage fermentation |
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