CN102106873B - Crocodile meat extract and preparation method and application thereof - Google Patents
Crocodile meat extract and preparation method and application thereof Download PDFInfo
- Publication number
- CN102106873B CN102106873B CN2010101423306A CN201010142330A CN102106873B CN 102106873 B CN102106873 B CN 102106873B CN 2010101423306 A CN2010101423306 A CN 2010101423306A CN 201010142330 A CN201010142330 A CN 201010142330A CN 102106873 B CN102106873 B CN 102106873B
- Authority
- CN
- China
- Prior art keywords
- crocodile
- artificially
- enzymolysis
- water
- crocodile meat
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a crocodile meat extract and a preparation method thereof. During the preparation, an enzymolysis mode is adopted to improve the content of arginine in a product; and according to screening experiment, the content of the arginine is highest in the crocodile meat extract which is subjected to secondary enzymolysis by adopting papain and pepsin, and the product has best mouthfeel and is applicable to further preparation of all kinds of health care food. The experiment further shows that: the crocodile meat extract has remarkable effect in terms of strengthening immunity and improving hypoxia tolerance.
Description
Invention field
The present invention relates to a kind of crocodile meat extract and preparation method thereof and the purposes aspect enhance immunity and raising anoxia endurance.
Background technology
The tradition traditional Chinese medicine has the record of treatment abdominal mass, and is also a lot of for the record of crocodile.Crocodiles, ancient dragon lizard, also known as Tuo fish ("Shen Nong's Herbal Classic").Chinese alligator meat is medicinal, and the beginning is stated from " Mingyi Bielu ": " main weak breath is inhaled, and foot is not on the spot." (weak breath is inhaled, and promptly refers to cough, asthma).Song dynasty " figure is through book on Chinese herbal medicine ": " southern people's Shiqi meat, the cloud look white is like chicken, but the gas chronic illness of feeling cold." Compendium of Material Medica claims: " meat is to help, and main weak breath is inhaled, and foot is not on the spot; The moisture perverse trend, all legendary venomous insects, abdominal mass, evil sore in the abdomen "." precious its meat of southern people is thought respecting of marriage ".Qing Dynasty's " medical center bun will " claims Chinese alligator meat " with enduring cream, routed heavily fortified point is drawn out pus by applying a plaster to the affected part, and goes stasis of blood myogenic ", mentions crocodile meat and endures the usage that cream is medicine.It is thus clear that brightly begun meat, the trusted subordinate abdominal mass deficient of crocodile clearly and disliked treatment such as sore tumour in treatment asthma, cough, rheumatism, health.1977 edition of "Chinese Dictionary" Indications are summarized its purpose as: "Governance Zhengjia, disease rickets, malignant sore fester."Guangdong is among the people to be had dietotherapy menus such as " tendril-leaved fritillary bulb are stewed crocodile meat " is used to cough, the supplemental treatment of asthma.
Crocodile meat can improve the blood oxygen carrying content, and cough, asthmatic patient lung oxygen exchange capacity are increased, and breathes easily smooth and easy.The Britain scientist discovers, the hemoglobin amino acid chain of crocodile muscle has unique chondritic, and this makes its hemoglobin oxygen carrying content surpass other animals more than 100 times, so the crocodile ability of in water, holding one's breath is very big.
Crocodile meat belongs to plain boiled pork, is rich in amino acid, aliphatic acid and the various trace elements of protein and needed by human.Protein accounts for 16.7% in the crocodile meat, and content is higher than pork, duck; Contain 18 seed amino acids in the crocodile meat, wherein 9 kinds is human essential amino acid, and content is abundant, and wherein the ratio of tryptophan, lysine, methionine is 1: 3: 3, meets the requirement of the United Nations to good protein, and its nutritive value surpasses other beastly meat of poultris; Containing 33 kinds of trace elements in the crocodile meat, wherein is the essential trace element favourable to the mankind more than 10 kinds, and iron and zinc content are higher.
Ancients obtain by the field with crocodile meat, existing crocodile meat in China, Thailand, Australia, the U.S. as food selling, be and propagate artificially.2005, but China national forestry bureau approval Nile crocodile, esturaine crocodile, three kinds of crocodiles of Siam crocodile are listed first batch of 54 kinds of business managements in and utilize the wild animal register.
Continuous expansion based on the crocodile aquaculture industry rationally utilizes the crocodile resource, and the further investigation of crocodile just seems more and more important with exploitation.This research passes through to extract crocodile meat active material, and this active material is carried out the pharmacodynamics screening, lays the foundation for further researching and developing.
Summary of the invention
The objective of the invention is to disclose a kind of crocodile meat extract, the present invention also aims to disclose this preparation method of extract, the present invention also aims to disclose the purposes of this extract aspect enhance immunity and raising anoxia endurance.
The present invention seeks to realize through following scheme.
Crocodile meat extract of the present invention is prepared from following method:
Get and propagate the crocodile fresh meat artificially, remove manadesma, be cut into 2 * 2cm fritter, the water flushing is placed in the steamer and is heated, and boiling continued heating 0.5～1.5 hour is taken out, and cools; Add 1-5 volume times deionized water, make crocodile meat and steam article homogenate, will steam article homogenate and be put in the enzymatic vessel, constant temperature 37-45 ℃ of water-bath heating after the 20-40 branch is planted, transferred PH=5～6; Than being the papain of 2-5%, enzymolysis 0.5～3 hour is put 70-90 ℃ of water bath with thermostatic control deactivation 10-20 minute, is cooled to 37 ℃, is incubated after 20-40 minute, transfers PH=1.4～1.8 at the bottom of the adding 10-30 volume enzyme doubly; Than being the pepsin of 2-5%, enzymolysis 2～5 hours is put 70-90 ℃ of water bath with thermostatic control deactivation 10-20 minute, reduces to room temperature at the bottom of the adding 10-30 volume enzyme doubly; Filter, get supernatant, in 110-130 ℃ of following moist heat sterilization 10-40 minute, nitrogen recharged, and after the sterilization, low temperature spray drying in the desinfection chamber is processed crocodile meat extract dry powder of the present invention, and is airtight, and low temperature drying is preserved.
Crocodile meat extract of the present invention preferably is prepared from following method:
Get and propagate the crocodile fresh meat artificially, remove manadesma, be cut into 2 * 2cm fritter, the water flushing is placed in the steamer and is heated, and boiling continued heating 1 hour is taken out, and cools; Add 2-4 volume times deionized water, make crocodile meat and steam article homogenate, will steam article homogenate and be put in the enzymatic vessel, constant temperature 37-45 ℃ of water-bath heating after 30 fens kinds, transferred PH=5～6; Add at the bottom of the 20 volumes enzyme doubly than the papain that is 3%, enzymolysis 1～2 hour is put 85 ℃ of water bath with thermostatic control deactivations 15 minutes, is cooled to 37 ℃, is incubated after 30 minutes accent PH=1.4～1.8; Add at the bottom of the 20 volumes enzyme doubly than the pepsin that is 3%, enzymolysis 4 hours is put 85 ℃ of water bath with thermostatic control deactivations 15 minutes, reduces to room temperature; Filter, get supernatant, in 121 ℃ of following moist heat sterilization 20-30 minutes, nitrogen recharged, and after the sterilization, low temperature spray drying in the desinfection chamber is processed crocodile meat extract dry powder of the present invention, and is airtight, and low temperature drying is preserved.
Wherein, above-mentionedly propagate crocodile artificially and preferably propagate Siam crocodile artificially.
Description of drawings
Fig. 1: ratio is investigated at the bottom of the pepsin enzymolysis enzyme
The invention discloses a kind of preparation method of crocodile meat extract; In the preparation process, adopt the mode of enzymolysis to improve the arginine content in the product; It is the highest to realize drawing the crocodile meat extract arginine content that adopts after papain and the pepsin secondary enzymolysis through screening; And the product mouthfeel is best, is applicable to further to make various health foods.Through realizing showing that further crocodile extract of the present invention is all having remarkable efficacy aspect enhance immunity and the raising anoxia endurance.
Following experimental example and embodiment are used to further specify but are not limited to the present invention.
Single factor of enzymolysis process is investigated experiment in the experimental example 1 crocodile meat method for distilling
Preparation crocodile meat steams article homogenate: get and propagate Siam's crocodile fresh meat artificially, remove manadesma, be cut into 2 * 2cm fritter, the water flushing is placed in the steamer and is heated, and boiling continued heating 1 hour is taken out, and cools; Add 2-4 volume times deionized water, make crocodile meat and steam article homogenate.
1, the selection of ratio at the bottom of the enzyme:
Get 18ml crocodile meat and steam article homogenate, in 50mL tool plug round-bottomed flask, add simulated gastric fluid to 50mL; Add respectively at the bottom of the enzyme than the pepsin that is 2%, 3%, 4%, 5% and 6%, enzymolysis 3 hours is put 85 ℃ of water bath with thermostatic control deactivations 15 minutes; After being cooled to room temperature, with 4200r/min centrifugal 15 minutes, get supernatant; With the arginine content is index, considers preparation cost again, and ratio is 3% at the bottom of finally definite enzyme.See table 1 and Fig. 1
Ratio is investigated at the bottom of the table 1 pepsin enzymolysis enzyme
2, the selection of enzymolysis liquid
(1) get 90ml crocodile meat and steam the article homogenate, on average be put in the 100ml tool plug round-bottomed flask, each flask is put 18ml; Add 32ml hydrochloric acid respectively; Airtight, in 50 ℃ of waters bath with thermostatic control, behind the 30min; In each flask, add pepsin, trypsase, papain, neutral proteinase, papain respectively and add neutral proteinase, concrete enzymatic hydrolysis condition press table 2 and is operated.Behind the 3h enzymolysis liquid is put 85 ℃ of waters bath with thermostatic control, deactivation 15min, be cooled to room temperature after, with the centrifugal 15min of 4200r/min, get supernatant, each need testing solution.With the arginine content is index.Use arginine--the ninhydrin decoration method is measured arginic content, and concrete grammar is following: precision is measured each need testing solution 0.5ml, respectively adds 2% ninhydrin solution and pH6.8 buffer solution 1ml, shakes up; Measure distilled water 0.5ml, add each 1ml of ninhydrin solution and phosphate buffer, shake up, as reagent blank liquid.100 ℃ of water-bath heating 15min take out cooling, are settled to 100ml with distilled water.Behind the 15min, measure absorbance at the 569nm place.The result sees table 3.
Table 2 enzyme class condition
Table 3 arginine-ninhydrin decoration method is investigated enzyme class table as a result
The result shows: arginic content pepsin group＞trypsase group＞neutral proteinase group＞papain+neutral enzymatic＞papain group in the single enzymolysis liquid, can find that thus pepsin enzymolysis arginine yield is the highest.
(2) the further screening experiment of pepsin, trypsase and papain
Get 162ml crocodile meat and steam the article homogenate, on average be put in the 100ml tool plug round-bottomed flask, each flask is put 18ml; Add 32ml hydrochloric acid respectively, airtight, 50 ℃ of waters bath with thermostatic control; Behind the 30min, in each flask, add pepsin, trypsase, papain enzymolysis respectively, after 1 hour enzymolysis liquid is put 85 ℃ of waters bath with thermostatic control; Deactivation 15min, be cooled to 37 ℃ the insulation 30min after, add pepsin, trypsase, papain respectively; After 3 hours enzymolysis liquid is put 85 ℃ of water bath with thermostatic control deactivation 15min, reduce to room temperature; Filter, get supernatant.According to the said method operation, concrete experimental design and result see table 4
Table 4 arginine-ninhydrin decoration method is investigated table as a result
The result shows: papain+pepsin group arginine content is the highest, this shows that the enzymolysis arginine yield of secondary enzymolysis protein is the highest.Simultaneously, the enzymolysis product mouthfeel that obtains of papain+pepsin group is best.
Experimental example 2 enzymolysis process optimization experiment
The crocodile meat of getting method preparation in the experimental example 1 steams article homogenate 162ml, on average is put in the 100ml tool plug round-bottomed flask, and each flask is put 18ml; Add 10,20 and 30 volumes simulated gastric fluid doubly respectively, airtight, respectively at 30 ℃, 37 ℃, 45 ℃ following water bath with thermostatic control effect 30min; Add respectively at the bottom of the enzyme than the pepsin that is 2%, 3%, 4%, 5%, 6%, enzymolysis 3h puts 85 ℃ of water bath with thermostatic control deactivation 15min with enzymolysis liquid; After being cooled to room temperature,, get supernatant with the centrifugal 15min of 4200r/min; With the arginine content is index, with temperature (℃), at the bottom of the enzyme than and hydrolysis time (h) be the investigation factor, press orthogonal design L
9(3
4) the table experiment arrangement, screening enzymolysis process parameter, see table 5 and table 6:
Table 5L
9(3
4) the factor level table
Table 6 orthogonal test and result
The above results is carried out variance analysis, and the result sees table 7:
Table 7 orthogonal test analysis of variance table
Annotate: F0.05 (2,8)=4.46, F0.01 (2,8)=8.65
The results of analysis of variance shows: ratio and hydrolysis time have conspicuousness influence (P＜0.01) to the result at the bottom of the enzyme.Consider production cost, confirm that finally the optimal parameter of crocodile meat enzymolysis is: ratio is 3%, 37 ℃ at the bottom of the enzyme, enzymolysis 4 hours, i.e. A
2B
1C
2
The crocodile meat extract natrium nitrosum poisoning mice anoxic experiment that experimental example 3 different enzymolysis process extract
1, experiment material: the ICR mouse, body weight 18～20g, male, provide by Beijing Vital River Experimental Animals Technology Co., Ltd..Natrium nitrosum (AG).Crocodile meat enzymolysis liquid is that wherein employed enzyme is respectively pepsin group, papain group and papain+pepsin like embodiment 1 said method preparation.
2, experimental procedure: mouse conforms and weighs after 1 week, is divided into 10 groups at random, and 12 every group, establish pepsin group, papain group, papain+pepsin group, be divided into big (2.4gkg
-1), in (1.2gkg
-1), little (0.24gkg
-1) three dose groups, giving 0.8ml crocodile meat enzymolysis liquid, the blank group gives 0.8ml distilled water and irritate stomach every day; Continuous irrigation stomach 30 days, 1 time/d.After the mouse last is irritated stomach 1h, ip (lumbar injection) 2% natrium nitrosum, 0.1ml/10g is an index with the breath stopped, the record mouse time-to-live.Experimental result show the heavy dose of group of crocodile meat enzymolysis liquid and in dose groups compare with blank control group that all there were significant differences, explain that crocodile meat enzymolysis liquid has the effect of raising anoxia endurance.Simultaneously papain+pepsin group also has some improvement than pepsin group, papain group, explains that the active material that this extraction process obtains is more, and drug effect is stronger.The result sees table 8
Table 8NaNO
2Poisoning mice anoxic experimental result (
N=12)
Compare with the blank group, P＜0.05 is represented with *
The activity research experiment of experimental example 4 crocodile meat extract enhance immunity power
1, experiment material and reagent
Person monocytic cell leukaemia THP-1 is available from U.S. cell bank ATCC, and cell culture medium is RPMI1640, and contains 2mM/L glutamine, 0.05Mm/L mercaptoethanol, 10% calf serum, two anti-solution (100 μ penicillin+100 μ streptomysins).RPMI-1640 (GIBCO company); Standard hyclone (Tianjin Hao ocean biological products company); Trypsase, tetrazolium bromide (MTT) and dimethyl sulfoxide (DMSO) (DMSO) are the Amresco Company products; TNF-alpha immunization kit is the R&D Company products, and VE, TNF-α standard items, LPS standard items, Lovastatin standard items are the Sigma Company products.BALB/c mouse: female, 18g is available from Department Of Medicine, Peking University's Experimental Animal Center.
The crocodile meat extract is prepared by embodiment 1 said method.
2, mouse T lymphocyte in-vitro multiplication test
The dislocation of BALB/c mouse cervical vertebra is put to death; Under the aseptic separation mouse bilateral jaw, under the clavicle, armpit, superficial inguinal lymph nodes and lymphonodi mesenterici; Placing precooling PBS 200 order nylon wires to grind filters; Collecting cell; Cold PBS is centrifugal, and (250g, 5min) washed cell is 2 times, is resuspended in RPMI1640 complete culture solution (25mmol/L glutamine, 100IU/mL penicillin, 100mg/L streptomysin, 50mol/L 3-mercaptoethanol, volume fraction 90%RPMI RPMI-1640 and 100mL/L hyclone) again.Lymphocyte after the washing is resuspended among the PBS, and counting and adjustment cell density are 1 * 10
7Cells/L; Be inoculated in 96 orifice plates, every pore volume 100 μ L, blank control group, control group (only adding ConA) and ConA are set respectively stimulates the experimental group of variable concentrations sample intervention down, and every group contains 5 multiple holes.Experimental group earlier with the crocodile meat extract sample (final concentration is 6250,3125,1560,780,390,97.5,48.75,24.5,12.3 μ g/mL) of variable concentrations at 37 ℃, 50mL/L CO
2Preincubate 4h in the incubator, in control group and the corresponding hole of experimental group, adding final concentration then is the ConA of 5mg/L.Incubator is hatched 72h.After cultivate finishing, every hole adds MTT 20 μ l, removes supernatant behind the reaction 4h, adds 150 μ lDMSO concussion dissolving and detects the A value at 490nm.Cell proliferation promotion rate=inhibiting rate=(A medicine/A contrast-1) * 100%.
Visible by table 9; In the mouse spleen lymphocyte nutrient solution of in vitro culture, add the crocodile meat extract, its concentration can significantly promote the T lymphocyte lymphopoiesis that ConA induces more than 97.5 μ g/ml, along with concentration increases; Facilitation is stronger, presents good dose-effect relationship.
Table 9 crocodile meat extract to the lymphopoietic influence of ConA activated T (average x ± s, n=3)
Compare P＜0.01 usefulness * * representes with the ConA control group
3, the crocodile meat extract discharges the inhibitory action of TNF-α to the THP-1 cell
(1) mensuration of TNF-α secretion inhibiting rate in the THP-1 cell
Cell suspension is diluted to 5 * 10
5Individual/ml, be planted in the 12 porocyte culture plates, every hole 1ml places incubator to cultivate.According to fixed concentration every hole dosing 100 μ l, and comprise positive Ve (final concentration 100 μ mol/L) and Lovastatin (final concentration 50 μ mol/L), 2 secondary holes of each concentration.Behind the dosing 1h, behind every hole adding Lps (final concentration is 50 μ g/L, and the blank group does not add), cultivate 4h in the incubator.Every porocyte liquid is transferred in the EP pipe, and centrifugal (3000r/min 15min), goes up cleer and peaceful precipitate and separate, is stored in respectively in-20 ℃ of refrigerators.Supernatant is used to measure the TNF-alpha content, and deposition is used for determining the protein quantity.
(2) the ELISA method is measured the TNF-alpha content
Get 8 EP pipe, utilize the kit buffer solution that TNF-α standard items are diluted respectively to be 1500,1000,500,250,125,100, the titer of 50pg/ml.Draw TNF-α calibration curve.Measure each sample TNF-alpha content according to the explanation of TNF-alpha immunization kit, and TNF-alpha content in the calculation sample.
(3) the Bradford method is measured cell protein content
Get 8 test tubes that 10mL is clean, press table 10 sampling, every pipe adds 400 μ l Coomassie brilliant blue dye liquors again; Abundant mixing; With cuvette colorimetric under the 595nm wavelength of 1cm light warp, each manages the optical density OD595nm that measures record, and makes calibration curve behind the placement 2min; Preparation cell protein sample: in filling the EP pipe of cell precipitation, add the NaOH of 150 μ l 0.1mol/L, cell lysis 5min, and instantly mixing.Centrifugal (15000r/min, 10min), it is subsequent use to get supernatant; Working sample protein content: get the clean test tube of some 10mL; Every pipe is got 50 μ l cell protein samples, adds 1.55ml distilled water, adds 400 μ l Coomassie brilliant blue dye liquors again; Abundant mixing; With cuvette colorimetric under the 595nm wavelength of 1cm light warp, each manages the optical density OD595nm that measures record behind the placement 2min, and checks in Protein content in the cell through calibration curve.
(4) calculating of TNF-α secretion inhibiting rate
TNF-α secretion inhibiting rate=(1-A/B) * 100%
A=sample TNF-α secretory volume/sample protein matter content
B=LPS group TNF-α secretory volume/LPS histone matter content
The result shows that the action effect of crocodile meat extract 390 μ g/ml has been equivalent to the effect of positive drug vitamin E (100 μ mol/L), and 5 concentration all can suppress the THP-1 cell and discharge TNF-α and show certain dose-effect correlation.Show that the crocodile meat extract has the activity that better inhibited THP-1 cell discharges TNF α.The result sees table 10.
The inhibition activity of table 10 crocodile meat extract inhibition Thp-1 cell release TNF-α (average x ± s, n=3)
bP＜0.01vs control group,
dP＜0.01vs LPS group.
Following embodiment all can realize the said effect of above-mentioned experimental example
The specific embodiment
Embodiment 1: crocodile meat extract preparation method of the present invention
Get and propagate Siam's crocodile fresh meat artificially, remove manadesma, be cut into 2 * 2cm fritter, the water flushing is placed in the steamer and is heated, and boiling continued heating 1 hour is taken out, and cools; Add 2-4 volume times deionized water, make crocodile meat and steam article homogenate, will steam article homogenate and be put in the enzymatic vessel, constant temperature 37-45 ℃ of water-bath heating after 30 fens kinds, transferred PH=5～6; Add at the bottom of the 20 volumes enzyme doubly than the papain that is 3%, enzymolysis 1～2 hour is put 85 ℃ of water bath with thermostatic control deactivations 15 minutes, is cooled to 37 ℃, is incubated after 30 minutes accent PH=1.4～1.8; Add at the bottom of the 20 volumes enzyme doubly than the pepsin that is 3%, enzymolysis 4 hours is put 85 ℃ of water bath with thermostatic control deactivations 15 minutes, reduces to room temperature; Filter, get supernatant, in 121 ℃ of following moist heat sterilization 20-30 minutes, nitrogen recharged, and after the sterilization, low temperature spray drying in the desinfection chamber is processed crocodile meat extract dry powder of the present invention, and is airtight, and low temperature drying is preserved.
Embodiment 2: crocodile meat extract preparation method of the present invention
Get and propagate the crocodile fresh meat artificially, remove manadesma, be cut into 2 * 2cm fritter, the water flushing is placed in the steamer and is heated, and boiling continued heating 1 hour is taken out, and cools; Add 3 volumes times deionized water, make crocodile meat and steam article homogenate, will steam article homogenate and be put in the enzymatic vessel, constant temperature 37-45 ℃ of water-bath heating after 20 fens kinds, transferred PH=5～6; Add at the bottom of the 30 volumes enzyme doubly than the papain that is 4%, enzymolysis 1.5 hours is put 85 ℃ of water bath with thermostatic control deactivations 20 minutes, is cooled to 37 ℃, is incubated after 20 minutes accent PH=1.4～1.8; Add at the bottom of the 30 volumes enzyme doubly than the pepsin that is 4%, enzymolysis 3 hours is put 70 ℃ of water bath with thermostatic control deactivations 20 minutes, reduces to room temperature; Filter, get supernatant, in 115 ℃ of following moist heat sterilizations 25 minutes, nitrogen recharged, and after the sterilization, low temperature spray drying in the desinfection chamber is processed crocodile meat extract dry powder of the present invention, and is airtight, and low temperature drying is preserved.
Claims (6)
1. propagate Siam's crocodile meat extract artificially for one kind, it is characterized in that this extract is prepared from following method:
Get and propagate the crocodile fresh meat artificially, remove manadesma, be cut into 2 * 2cm fritter, the water flushing is placed in the steamer and is heated, and boiling continued heating 0.5～1.5 hour is taken out, and cools; Add 1-5 volume times deionized water, make crocodile meat and steam article homogenate, will steam article homogenate and be put in the enzymatic vessel, constant temperature 37-45 ℃ of water-bath heating after the 20-40 branch is planted, transferred PH=5～6; Than being the papain of 2-5%, enzymolysis 0.5～3 hour is put 70-90 ℃ of water bath with thermostatic control deactivation 10-20 minute, is cooled to 37 ℃, is incubated after 20-40 minute, transfers PH=1.4～1.8 at the bottom of the adding 10-30 volume enzyme doubly; Than being the pepsin of 2-5%, enzymolysis 2～5 hours is put 70-90 ℃ of water bath with thermostatic control deactivation 10-20 minute, reduces to room temperature at the bottom of the adding 10-30 volume enzyme doubly; Filter, get supernatant, in 110-130 ℃ of following moist heat sterilization 10-40 minute, nitrogen recharged, and after the sterilization, low temperature spray drying in the desinfection chamber is processed crocodile meat extract dry powder, and is airtight, and low temperature drying is preserved.
2. Siam's crocodile meat extract of propagating artificially as claimed in claim 1 is characterized in that this extract is prepared from following method:
Get and propagate the crocodile fresh meat artificially, remove manadesma, be cut into 2 * 2cm fritter, the water flushing is placed in the steamer and is heated, and boiling continued heating 1 hour is taken out, and cools; Add 2-4 volume times deionized water, make crocodile meat and steam article homogenate, will steam article homogenate and be put in the enzymatic vessel, constant temperature 37-45 ℃ of water-bath heating after 30 fens kinds, transferred PH=5～6; Add at the bottom of the 20 volumes enzyme doubly than the papain that is 3%, enzymolysis 1～2 hour is put 85 ℃ of water bath with thermostatic control deactivations 15 minutes, is cooled to 37 ℃, is incubated after 30 minutes accent PH=1.4～1.8; Add at the bottom of the 20 volumes enzyme doubly than the pepsin that is 3%, enzymolysis 4 hours is put 85 ℃ of water bath with thermostatic control deactivations 15 minutes, reduces to room temperature; Filter, get supernatant, in 121 ℃ of following moist heat sterilization 20-30 minutes, nitrogen recharged, and after the sterilization, low temperature spray drying in the desinfection chamber is processed crocodile meat extract dry powder, and is airtight, and low temperature drying is preserved.
3. the preparation method who propagates Siam's crocodile meat extract artificially as claimed in claim 1 is characterized in that this method comprises the steps:
Get and propagate the crocodile fresh meat artificially, remove manadesma, be cut into 2 * 2cm fritter, the water flushing is placed in the steamer and is heated, and boiling continued heating 0.5～1.5 hour is taken out, and cools; Add 1-5 volume times deionized water, make crocodile meat and steam article homogenate, will steam article homogenate and be put in the enzymatic vessel, constant temperature 37-45 ℃ of water-bath heating after the 20-40 branch is planted, transferred PH=5～6; Than being the papain of 2-5%, enzymolysis 0.5～3 hour is put 70-90 ℃ of water bath with thermostatic control deactivation 10-20 minute, is cooled to 37 ℃, is incubated after 20-40 minute, transfers PH=1.4～1.8 at the bottom of the adding 10-30 volume enzyme doubly; Than being the pepsin of 2-5%, enzymolysis 2～5 hours is put 70-90 ℃ of water bath with thermostatic control deactivation 10-20 minute, reduces to room temperature at the bottom of the adding 10-30 volume enzyme doubly; Filter, get supernatant, in 110-130 ℃ of following moist heat sterilization 10-40 minute, nitrogen recharged, and after the sterilization, low temperature spray drying in the desinfection chamber is processed crocodile meat extract dry powder, and is airtight, and low temperature drying is preserved.
4. the preparation method who propagates Siam's crocodile meat extract artificially as claimed in claim 3 is characterized in that this method comprises the steps:
Get and propagate the crocodile fresh meat artificially, remove manadesma, be cut into 2 * 2cm fritter, the water flushing is placed in the steamer and is heated, and boiling continued heating 1 hour is taken out, and cools; Add 2-4 volume times deionized water, make crocodile meat and steam article homogenate, will steam article homogenate and be put in the enzymatic vessel, constant temperature 37-45 ℃ of water-bath heating after 30 fens kinds, transferred PH=5～6; Add at the bottom of the 20 volumes enzyme doubly than the papain that is 3%, enzymolysis 1～2 hour is put 85 ℃ of water bath with thermostatic control deactivations 15 minutes, is cooled to 37 ℃, is incubated after 30 minutes accent PH=1.4～1.8; Add at the bottom of the 20 volumes enzyme doubly than the pepsin that is 3%, enzymolysis 4 hours is put 85 ℃ of water bath with thermostatic control deactivations 15 minutes, reduces to room temperature; Filter, get supernatant, in 121 ℃ of following moist heat sterilization 20-30 minutes, nitrogen recharged, and after the sterilization, low temperature spray drying in the desinfection chamber is processed crocodile meat extract dry powder, and is airtight, and low temperature drying is preserved.
5. the preparation method who propagates Siam's crocodile meat extract artificially as claimed in claim 3 is characterized in that wherein propagating artificially crocodile for propagating Siam crocodile artificially.
6. according to claim 1 or claim 2 Siam's crocodile meat extract of propagating artificially has enhance immunity or improves the application in the medicine of anoxia endurance effect in preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101423306A CN102106873B (en) | 2010-04-07 | 2010-04-07 | Crocodile meat extract and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101423306A CN102106873B (en) | 2010-04-07 | 2010-04-07 | Crocodile meat extract and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102106873A CN102106873A (en) | 2011-06-29 |
| CN102106873B true CN102106873B (en) | 2012-07-04 |
Family
ID=44171229
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101423306A Active CN102106873B (en) | 2010-04-07 | 2010-04-07 | Crocodile meat extract and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102106873B (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102578602A (en) * | 2012-03-14 | 2012-07-18 | 宿迁润宝食品有限公司 | Enzymic method for extracting meat extract |
| CN102793052B (en) * | 2012-08-13 | 2014-01-15 | 广西盟展鳄鱼科技开发有限公司 | Crocodile meat protein antioxidative peptide powder and preparation method thereof |
| CN103393739A (en) * | 2013-08-15 | 2013-11-20 | 汪华东 | Crocodile penis composition for enhancing sexual function and tonifying kidney and preparation method thereof |
| CN103416768B (en) * | 2013-08-15 | 2015-06-17 | 汪华东 | Ziyin Busheng crocodile meat composition and preparation method thereof |
| CN103767030A (en) * | 2014-01-24 | 2014-05-07 | 蔡建中 | Preparation method of crocodile beverage |
| CN104687055A (en) * | 2015-03-30 | 2015-06-10 | 广州善康生物科技有限公司 | Crocodile composition capable of losing weight and preparation method thereof |
| CN104687098B (en) * | 2015-03-30 | 2017-10-10 | 广州善康生物科技有限公司 | It is a kind of to reduce crocodile composite of blood glucose and preparation method thereof |
| CN108651666A (en) * | 2018-04-27 | 2018-10-16 | 广州善康生物科技有限公司 | A kind of crocodile composite and preparation method thereof that can be refreshed oneself |
| CN108552441A (en) * | 2018-04-27 | 2018-09-21 | 广州善康生物科技有限公司 | A kind of crocodile beverage composition and preparation method thereof that can remove fishy smell |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1147913A (en) * | 1996-08-08 | 1997-04-23 | 胡健民 | Concentrated soup made of turtle, soft-shelled turtle and snake |
| CN1160542A (en) * | 1996-11-11 | 1997-10-01 | 朱圣田 | Liquid crocodile composite and its preparation |
| CN1160565A (en) * | 1996-11-11 | 1997-10-01 | 朱圣田 | Crocodile composite and its preparation process |
-
2010
- 2010-04-07 CN CN2010101423306A patent/CN102106873B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN102106873A (en) | 2011-06-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102106873B (en) | Crocodile meat extract and preparation method and application thereof | |
| CN100536878C (en) | Tianshen oral liquid with fatigue-resistance and immunoregulation action | |
| CN104531477A (en) | Peony kidney-nourishing wine and preparation method thereof | |
| CN109757733A (en) | A kind of oral polypeptide powder and its preparation method and application | |
| CN103408634A (en) | Horse placenta water-soluble protein extract, preparation method and application thereof | |
| CN105017438B (en) | A kind of Radix et Rhizoma Gynurae divaricatae polysaccharide and its application in preparing for immunological regulation and anti-tumor drug and functional food | |
| CN108164615A (en) | A kind of preparation method and application of Siberian solomonseal rhizome polysaccharide | |
| CN104905237A (en) | Inonotus obliquus composite electuary and production method thereof | |
| CN104388236A (en) | Grape wine as well as preparation method and application thereof | |
| CN106834404B (en) | Enzymolysis polypeptide and application thereof in preparing medicament for treating lung adenocarcinoma | |
| CN1977662A (en) | New use of Chinese angelica, ginger mutton soup | |
| CN103330211A (en) | Composition with immunoregulation function and preparation method and application of composition | |
| CN103301161B (en) | The method of Chinese medicine preparation and the application of this Chinese medicine preparation is prepared with Eupolyphaga seu steleophaga extract | |
| CN106135878A (en) | Jilin Radix Ginseng oligopeptide purposes in preparation improves the food with sexual function improving or health food | |
| JP2005502597A (en) | Extract from South Korea | |
| CN103636875A (en) | Anti-cancer herbal tea | |
| Susanto et al. | Efficacy of moringa oleifera leaf powder as nutrigenomic therapy against malnutrition and metabolic perturbation related diseases: a preliminary study of madura islands variety | |
| CN102311905A (en) | Health-care wine and preparation thereof | |
| CN100544736C (en) | A kind of pharmaceutical composition of enhancing immunity and preparation method | |
| CN106668077A (en) | Marine bioactive composition and pharmaceutical preparation | |
| CN104027447B (en) | A kind of Chinese medicine of Therapeutic cancer | |
| CN103141843B (en) | Herba cistanches and cynomorium songaricum health-care sustained-release capsule | |
| CN102268414A (en) | Coprinus comatus laccase with activity of inhibiting tumor cell proliferation and preparation method thereof | |
| CN104255922A (en) | Health care milk suitable for three-high crowd to drink and preparation method of milk | |
| CN103156194B (en) | Medlar and tuckahoe capsules and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |