CN102106589A - Lysozyme preservative and application thereof in preservation of aquatic products - Google Patents
Lysozyme preservative and application thereof in preservation of aquatic products Download PDFInfo
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- CN102106589A CN102106589A CN2009102007536A CN200910200753A CN102106589A CN 102106589 A CN102106589 A CN 102106589A CN 2009102007536 A CN2009102007536 A CN 2009102007536A CN 200910200753 A CN200910200753 A CN 200910200753A CN 102106589 A CN102106589 A CN 102106589A
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- lysozyme
- antistaling agent
- aquatic products
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Abstract
The invention relates to a lysozyme preservative and application thereof in preservation of aquatic products. The lysozyme preservative comprises the following components in percentage by mass based on 100 grams of water: 0.01 to 0.1 percent of lysozyme, 0.5 to 1.5 percent of sodium chloride, 4 to 8 percent of glycocoll, 0.01 to 0.1 percent of potassium sorbate, 0.1 to 0.5 percent of ascorbic acid and 0.01 to 0.05 percent of phytic acid. A preparation method comprises the following steps of: cleaning fresh aquatic product samples, removing the individuals which are large in difference, grouping the rest samples randomly for preservation; and adding the aquatic product samples into the lysozyme preservative in a mass ratio of 1:0.5-1:1.5, and immersing for 20 to 100 seconds. The lysozyme preservative has the advantages of sanitation, safety, obvious effect and long quality guarantee period.
Description
[technical field]
The present invention relates to the lysozyme technical field, specifically, is a kind of lysozyme antistaling agent and the application in aquatic products fresh-keeping thereof.
[background technology]
Lysozyme (lysozyme) is a kind of hydrolase that acts on microorganism wall specially, claims muramidase (murami2dase) again.People started from the research of lysozyme at the beginning of last century, and Britain bacteriologist Flem2ing has the enzyme of dissolution of bacteria cell membrane in preceding 6 years (nineteen twenty-two) finders' that find penicillin saliva, tears, because of it has bacteriolysis, so the called after lysozyme.After this people have also found the existence of lysozyme in the multiple tissue of humans and animals, secretion, certain plants, microorganism.Wherein the research of HEL and application are quite deeply with extensive.In addition along with the development of technique for gene engineering, by synthetic gene, among the research of producing human lysozyme with microbial fermentation is also deeply being carried out.
The alkaline globulin that lysozyme is made up of 129 amino acid residues, single peptide chain, molecular weight are 14388, molecule contains four pairs of disulfide bond; The activity of lysozyme center is Asp52 and Glu35; It can catalyzing hydrolysis N-acetylglucosamine and-acetylmuramic acid between and β-1,4 glycosidic bond between the N-acetylglucosamine, lysozyme is a kind of more stable protein.
[summary of the invention]
The objective of the invention is to overcome the deficiencies in the prior art, a kind of lysozyme antistaling agent and the application in aquatic products fresh-keeping thereof are provided.
The objective of the invention is to be achieved through the following technical solutions:
The application of a kind of lysozyme antistaling agent in aquatic products fresh-keeping, its concrete steps are:
A kind of component of lysozyme antistaling agent is formed mass percent, calculates according to 100 gram water;
Lysozyme 0.01～0.1%
Sodium chloride 0.5～1.5%
Glycine 4～8%
Potassium sorbate 0.01～0.1%
Ascorbic acid 0.1～0.5%
Phytic acid 0.01～0.05%
As the preferred technical solution of the present invention, the component of described lysozyme antistaling agent consists of,
Lysozyme 0.03～0.07%
Sodium chloride 0.75～1.25%
Glycine 5～7%
Potassium sorbate 0.03～0.8%
Ascorbic acid 0.2～0.4%
Phytic acid 0.02～0.04%
As most preferred technique scheme of the present invention, the component of described lysozyme antistaling agent consists of,
Lysozyme 0.05%
Sodium chloride 1%
Glycine 6%
Potassium sorbate 0.06%
Ascorbic acid 0.3%
Phytic acid 0.01%
Described glycine (Glycine) has another name called amion acetic acid, molecular formula [C
2H
4O
2N]
2
The molecular formula C of described potassium sorbate
6H
7KO
2
The molecular formula of described ascorbic acid is C
6H
8O
6
Described phytic acid (Phytic acid) has another name called phytic acid, and molecular formula is C
6H
18O
24P
6
A kind of preparation method of lysozyme antistaling agent, above-mentioned raw materials is soluble in water, obtain the lysozyme antistaling agent.
The application of a kind of lysozyme antistaling agent in aquatic products fresh-keeping, its concrete steps are: get fresh aquatic products sample, as hairtail, squid etc.; Sample is cleaned, remove the individuality that differs greatly, then with the sample random packet, as fresh-keeping use; The aquatic products sample is joined in the lysozyme antistaling agent, and the intrusion time is 20～100s, and the intrusion time is preferably 60s; The mass ratio of aquatic products sample and lysozyme antistaling agent is 1: 0.5～1: 1.5, and its mass ratio is preferably 1: 1;
Compared with prior art, good effect of the present invention is:
At present, the aquatic products preservation of China is freezed fresh-keeping method with main application of circulation, and the goods that comprise refrigeration (more than 0 ℃) on the supermarket shelves seldom; Freezing meeting causes the destruction of protein denaturation and matter structure, local flavor is produced harmful effect, but common refrigerated shelf life is shorter, makes aquatic products be difficult to into the people field of circulating; Utilize the lysozyme composite preservative to combine, both can extend the shelf life, do not make the matter structure and the local flavor sustain damage of aquatic products again with refrigeration, and health, safety.
[specific embodiment]
The specific embodiment of a kind of lysozyme antistaling agent of the present invention and the application in aquatic products fresh-keeping thereof below is provided.
Embodiment 1
A kind of lysozyme antistaling agent and the application in hairtail fresh-keeping thereof
(1) a kind of lysozyme antistaling agent
A kind of component of lysozyme antistaling agent is formed mass percent, calculates according to 100 gram water;
Lysozyme 0.01%
Sodium chloride 0.5%
Glycine 4%
Potassium sorbate 0.01%
Ascorbic acid 0.1%
Phytic acid 0.01%
Above-mentioned raw materials is soluble in water, obtain the lysozyme antistaling agent.
(2) application of a kind of lysozyme antistaling agent in hairtail fresh-keeping
Get fresh aquatic products sample,, sample is cleaned, remove the individuality that differs greatly, then with the sample random packet, as fresh-keeping use as hairtail; Join the lysozyme antistaling agent, the intrusion time is 20s; The mass ratio of aquatic products sample and lysozyme antistaling agent is 1: 0.5.
Embodiment 2
A kind of lysozyme antistaling agent and the application in squid fresh-keeping thereof
(1) a kind of lysozyme antistaling agent
A kind of component of lysozyme antistaling agent is formed mass percent, calculates according to 100 gram water;
Lysozyme 0.05%
Sodium chloride 1%
Glycine 6%
Potassium sorbate 0.06%
Ascorbic acid 0.3%
Phytic acid 0.01%
Above-mentioned raw materials is soluble in water, obtain the lysozyme antistaling agent.
(2) application of a kind of lysozyme antistaling agent in squid fresh-keeping
Get fresh aquatic products sample,, sample is cleaned, remove the individuality that differs greatly, then with the sample random packet, as fresh-keeping use as squid; Join the lysozyme antistaling agent, the intrusion time is 60s; The mass ratio of aquatic products sample and lysozyme antistaling agent is 1: 1.
Embodiment 3
A kind of lysozyme antistaling agent and the application in scallop post fresh-keeping thereof
(1) a kind of lysozyme antistaling agent
A kind of component of lysozyme antistaling agent is formed mass percent, calculates according to 100 gram water;
Lysozyme 0.1%
Sodium chloride 1.5%
Glycine 8%
Potassium sorbate 0.1%
Ascorbic acid 0.5%
Phytic acid 0.05%
Above-mentioned raw materials is soluble in water, obtain the lysozyme antistaling agent.
(2) application of a kind of lysozyme antistaling agent in scallop post fresh-keeping
Get fresh aquatic products sample,, sample is cleaned, remove the individuality that differs greatly, then with the sample random packet, as fresh-keeping use as the scallop post; Join the lysozyme antistaling agent, the intrusion time is 100s; The mass ratio of aquatic products sample and lysozyme antistaling agent is 1: 1.5.
Antistaling agent safe and sanitary of the present invention, effect significantly, and along with the continuity of preservation time to add the effect of composite preservative obvious more; Fresh-keeping mechanism mainly is to suppress the putrefactive microorganisms growth, and this can be confirmed from the obvious minimizing of total number of bacteria.
The above only is a preferred implementation of the present invention; should be pointed out that for those skilled in the art, without departing from the inventive concept of the premise; can also make some improvements and modifications, these improvements and modifications also should be considered within the scope of protection of the present invention.
Claims (7)
1. a lysozyme antistaling agent is characterized in that, component is formed mass percent and is, calculates according to 100 gram water;
Lysozyme 0.01～0.1%
Sodium chloride 0.5～1.5%
Glycine 4～8%
Potassium sorbate 0.01～0.1%
Ascorbic acid 0.1～0.5%
Phytic acid 0.01～0.05%.
2. a kind of lysozyme antistaling agent as claimed in claim 1 is characterized in that the component of described lysozyme antistaling agent consists of,
Lysozyme 0.03～0.07%
Sodium chloride 0.75～1.25%
Glycine 5～7%
Potassium sorbate 0.03～0.8%
Ascorbic acid 0.2～0.4%
Phytic acid 0.02～0.04%.
3. a kind of lysozyme antistaling agent as claimed in claim 1 is characterized in that the component of described lysozyme antistaling agent consists of,
Lysozyme 0.05%
Sodium chloride 1%
Glycine 6%
Potassium sorbate 0.06%
Ascorbic acid 0.3%
Phytic acid 0.01%.
4. the preparation method of a kind of lysozyme antistaling agent as claimed in claim 1 is characterized in that, above-mentioned raw materials is soluble in water, obtains the lysozyme antistaling agent.
5. the lysozyme antistaling agent application in aquatic products fresh-keeping is characterized in that concrete steps are: get fresh aquatic products sample; Sample is cleaned, remove the individuality that differs greatly, then with the sample random packet, as fresh-keeping use; The aquatic products sample is joined in the lysozyme antistaling agent, and the intrusion time is 20～100s; The mass ratio of aquatic products sample and lysozyme antistaling agent is 1: 0.5～1: 1.5.
6. the application of a kind of lysozyme antistaling agent as claimed in claim 5 in aquatic products fresh-keeping is characterized in that the described intrusion time is 60s.
7. the application of a kind of lysozyme antistaling agent as claimed in claim 5 in aquatic products fresh-keeping is characterized in that the mass ratio of described aquatic products sample and lysozyme antistaling agent is 1: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102007536A CN102106589A (en) | 2009-12-25 | 2009-12-25 | Lysozyme preservative and application thereof in preservation of aquatic products |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102007536A CN102106589A (en) | 2009-12-25 | 2009-12-25 | Lysozyme preservative and application thereof in preservation of aquatic products |
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| CN102106589A true CN102106589A (en) | 2011-06-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2009102007536A Pending CN102106589A (en) | 2009-12-25 | 2009-12-25 | Lysozyme preservative and application thereof in preservation of aquatic products |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102389151A (en) * | 2011-11-09 | 2012-03-28 | 上海海洋大学 | Preparation method of biological preservative for Penaeus vannamei |
| CN103636773A (en) * | 2013-12-06 | 2014-03-19 | 中国农业科学院农产品加工研究所 | Preservation and freshness-keeping method for winter jujubes |
| CN109287731A (en) * | 2017-07-25 | 2019-02-01 | 肖佳楠 | A kind of plyability meat poultry food preservative |
-
2009
- 2009-12-25 CN CN2009102007536A patent/CN102106589A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102389151A (en) * | 2011-11-09 | 2012-03-28 | 上海海洋大学 | Preparation method of biological preservative for Penaeus vannamei |
| CN102389151B (en) * | 2011-11-09 | 2013-04-17 | 上海海洋大学 | Preparation method of biological preservative for Penaeus vannamei |
| CN103636773A (en) * | 2013-12-06 | 2014-03-19 | 中国农业科学院农产品加工研究所 | Preservation and freshness-keeping method for winter jujubes |
| CN103636773B (en) * | 2013-12-06 | 2015-03-04 | 中国农业科学院农产品加工研究所 | Preservation and freshness-keeping method for winter jujubes |
| CN109287731A (en) * | 2017-07-25 | 2019-02-01 | 肖佳楠 | A kind of plyability meat poultry food preservative |
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Application publication date: 20110629 |
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