CN102106256B - Hybrid seed production method for tobacco - Google Patents
Hybrid seed production method for tobacco Download PDFInfo
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- CN102106256B CN102106256B CN 201110023476 CN201110023476A CN102106256B CN 102106256 B CN102106256 B CN 102106256B CN 201110023476 CN201110023476 CN 201110023476 CN 201110023476 A CN201110023476 A CN 201110023476A CN 102106256 B CN102106256 B CN 102106256B
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Abstract
The invention discloses a hybrid seed production method for tobacco, which comprises the following steps of: planting a male parent and a female parent of the tobacco separately; adjusting a blooming stage by using a seedling stage to make the male parent blossoms 10-20 days earlier than the female parent; after the initial blooming stage of the male parent, picking flowers with a non-dehiscent anther everyday and collecting and storing pollen for later use; when a female parent plant is in a full-bloom stage, cutting fruits on the selected tobacco plant with only the flowers and buds left; tearing off blossomed corollas to expose stigmas; coating the pollen of the male parent on the stigmas of the female parent uniformly; and remaining 80-100 pollinated remaining fruits per plant. In the method, links such as a planting manner, a planting ratio, the blooming stage, male parent pollen extraction time, a male parent extraction method, pollination time, a pollination method, a female parent fruit remaining number per plant and the like are improved, so that the efficiency and the quality of tobacco hybrid seed production are increased, the planting area of the male parents of the tobacco is reduced, the amount of labor used in artificial supplementary pollination of hybrid seed production is reduced, the pollination efficiency is increased, the seed production yield is increased, and the production cost of the hybrid seed production is reduced.
Description
Technical field
The present invention relates to the cross-breeding method of plant, particularly relate to a kind of hybrid seed production method for tobacco.
Background technology
The flue-cured tobacco heterosis utilization is attempted already in history, and is achieved success to a certain extent; But, for the heterotic utilization of flue-cured tobacco, exist also difference on the understanding (fourth high sea 1976, the Kubo friend is bright 1982, James and Chaplin 1966, Akehurst 1981, Lucas 1975 etc. for Tong road scholar 1980, Ai Shu reason 1987) always.Understanding difference part is two aspects: the one, and the flue-cured tobacco crossbreed has or not the problem of quality trait advantage.By the detailed research of many scholars, now substantially reach common understanding, think that flue-cured tobacco hybrid vigour is mainly manifested in parents' advantage complementary, comprehensive parents' different resistances are in one; Solve quality and resistance contradiction, quality of tobacco is good; Environmental suitability is strong, applied range; Accelerate breed breeding, optionally in the production increase with kind; Control free breeding, be easy to accomplish that location, kind producing region produces, guarantee the aspects such as raw material of industry demand.The 2nd, the production of hybrid seeds problem of flue-cured tobacco crossbreed is that mainly none overlaps effective seeding technique measure, except Kubo friend bright (1982) had report aspect pollination efficiency, yet there are no the research report of relevant cross-breeding method and technology.Male sterile line is less on the one hand, and the parental apolegamy scope is narrow.Owing to utilizing hybrid vigour on most breeder's disapprove tobaccos, cause the male sterile line transformation to get the brush-off, existing male sterile line resource seldom, and the production of crossbreed, need again male sterile line, thoroughly do not cause impurity of seeds and to improve seed production efficiency to avoid castrating, so be subjected to the restriction of parent's range of choice when breeding hybridized, the effect of heterosis utilization is little.Hybrid seed production yields is low on the other hand, efficient is low, benefit is low.The tobacco seed reproduction coefficient is high, floral organ is large, florescence length is the advantage of the tobacco hybridization production of hybrid seeds.And the production of hybrid seeds problem of crossbreed is that mainly none overlaps effective seeding technique measure, causes the not enough 2kg of production of hybrid seeds per mu yield, the not enough 100g of every worker seeding every day, and 3000 yuan every mu seed income from sales is removed cost and is remained little, and can talk without production of hybrid seeds benefit.Therefore, be necessary to carry out seed selection and the research of male sterile cross-breeding technology of male sterile line.
The problem that tradition flue-cured tobacco cross-breeding method and technology exist: 1, yield poorly.At first, this method of operating adopts Parent planting proportion 1:1 or 1:2, and maternal ratio is very few, and the plant quantity of every mu of results hybrid seed is few.Secondly, it is 60 fruits that this specification requirement individual plant stays the fruit number, is not the best joint of seed quality and output under the existing planting conditions, and individual plant outstanding achievement number is on the low side, causes seed yield per plant low.The 3rd, this specification requirement Parent flower synchronization, and adopt to concentrate first and adopt powder, and then the mode of unified pollination, therefore can not hybridize at paternal pollen and maternal the strongest time of ovule vitality, the outstanding achievement fecundity is poor.The seed per mu yield that this technology is used is generally 1-2kg.2, efficient is low.According to test, select 60 flowers to carry out artificial pollination at a strain male sterile plants, collection pollen and hybridization required time are approximately every strain 10～15 minutes, get powder, 20 workers of pollination needs for every mu, amount to every worker seeding every day 50-100g, therefore affected greatly production efficiency.
Summary of the invention
Technical problem to be solved by this invention is: a kind of hybrid seed production method for tobacco is provided, it gets powder time and method to planting patterns, planting proportion, florescence, the male parent of Parent, pollination time and method and maternal individual plant stay link such as fruit number etc. to improve, to improve efficient and the quality of the tobacco hybridization production of hybrid seeds.
The present invention is achieved in that hybrid seed production method for tobacco, with the male parent of tobacco and maternal separately plantation, and regulates the florescence with date of seeding, makes male parent than maternal prematurity 10～20 days; Behind the male parent initial bloom stage, take off the flower that flower pesticide does not split every day, collect pollen storage for subsequent use; When maternal plant is in full-bloom stage, the fruit of choosing the cigarette strain to tie is cut, only stay flower and bud, tear the corolla of having bloomed, expose column cap, paternal pollen is evenly spread upon on the maternal column cap; Keeping the individual plant pollination to stay the fruit number is 80～100 fruits.
Male parent is 1:10～20 with the planting proportion of female parent;
Behind the male parent initial bloom stage, before 10 of every days, take off the flower that to open the same day, at indoor concentrated taking-up flower pesticide, thickness with 1～2cm is tiled in the culture dish, then be placed on make Anther dehiscence in the aeration cabinet of the local of aeration-drying or 34～36 ℃ after, remove anther wall with 50～100 purpose stainless steel sifts sieves, only stay pollen, be placed on again in the refrigerator that is kept at 2～4 ℃ in the drier storage for subsequent use.
At maternal full-bloom stage, after 10 of fine mornings, select on the maternal plant that corolla is little redly to split to corolla, and column cap is that the flowers of 2 row or 3 row are pollinated.
When pollinating, adopt the writing brush semar technique to pollinate, evenly spread upon on the maternal column cap with the drunk paternal pollen of writing brush.
Make the full-bloom stage of maternal plant be in late July.
In order to verify effect of the present invention, adopt technical scheme of the present invention and prior art to compare, do following research:
(3.1) the column cap vigor is on the impact of hybrid seeding
The column cap vigor affects the solid of hybrid seeding by affecting pollen germination.Column cap is energetic, promotes that the nutriment of pollen germination is many, and the easy sprouting of pollen is stretched into ovary and finished insemination, and hybrid seeding is solid good; On the contrary, a little less than the column cap vigor, hybrid seeding is solid poor.
(3.1.1) the column cap shape is on the impact of column cap vigor
Adopt the column cap in 3 kinds of different periods to compare, according to the column cap shape of flue-cured tobacco male sterile line and before us to the energetic weak preliminary observation result of various shape column caps, the column cap shape is divided into 3 classes, a class (2 row), two classes (3 row), three classes (all the shape column caps except 2,3 row).By the combination assembly, in full-bloom stage with fresh pollen to the three class column caps of blooming the same day pollination of listing respectively, 80 of every processing, then three repetitions record its solid situation such as table 1.
The solid situation of the different column cap shapes of table 1
Found out by table 1, the fruiting rate of a class column cap and average solid seed number are maximum, and two class column caps take second place, and three class column caps are the poorest.Without significant difference, and between three class column caps utmost point significant difference is arranged between one class column cap and two class column caps, may split with the style majority of three class column caps, pollen tube can't stretch into ovary, and to finish insemination relevant.Therefore, the column cap vigor of 2 row shapes is the strongest, and the column cap vigor of 3 row shapes is slightly inferior, and the column cap vigor of three class shapes is the most weak.
(3.1.2) the flowers are in blossom fate is on the impact of column cap vigor
Test establishes that the flowers are in blossom front 3 days (code name-3), 2 days (code name-2), 1 day (code name-1), the flowers are in blossom same day (code name 0), the flowers are in blossom rear 1 day (code name 1), 2 days (code name 2), 3 days (code name 3) seven kinds of processing., its result such as table 2.
Solid situation and the multiple range test of the pollination of table 2 the flowers are in blossom different number of days
Found out by table 2: the fruiting rate of different number of days that the flowers are in blossom pollination: 0〉1 〉-1〉2 〉-2〉3 〉-3.Between 0 and-1,1 without significant difference, between 0 ,-1,1,2 ,-2 without the extreme difference significant difference; The average solid seed number of different number of days that the flowers are in blossom pollination: between 0 〉-1〉1 〉-2〉2 〉-3〉3,0 and-1,1 without significant difference, between 0,1 ,-1 ,-2 without the extreme difference significant difference.Explanation is for each flower, and column cap vigor front 1 day from the flowers are in blossom rear 1 day to the flowers are in blossom is strong, the flowers are in blossom front 2 days to the flowers are in blossom rear 2 days column caps still have stronger vigor.
By the solid situation of above different column cap shapes, the flowers are in blossom different number of days and the results of analysis of variance as can be known, hybrid seeding should be selected 2 row and 3 colonnade heads, and in the flowers are in blossom front 1 day to the flowers are in blossom pollination in rear 1 day 3 days, special such as weather condition, also can the flowers are in blossom front 2 days to the flowers are in blossom pollination in rear 2 days.
(3.2) store Pollen Activity to the impact of hybrid seeding
It is to solve male parent early blossoming in the hybrid seeding or bad weather cause the pollinating requisite measure of difficulty that pollen is stored, so Study of Pollens is stored hybrid seeding is had very important effect.The storage Pollen Activity is subjected to the impact of storage requirement and period of storage, and low temperature, low humidity and short period store, and are conducive to keep Pollen Activity.The mensuration of Pollen Activity can adopt dyeing and germinate and directly measure, and also can adopt field pollination indirect determination.Employing field pollination indirect determination Pollen Activity, accuracy is high, and is simple, as long as the pollen that will measure is taken the field pollination, strong and weak according to the solid Pollen Activity of how much just can differentiating behind the seed maturity.The solid situation that now different storage conditions and the pollen of depositing fate is used for the field pollination is listed in table 3.
The solid situation of pollination after table 3 pollen is deposited
Found out by table 3: the fruiting rate of contrast and ambient temperature and moisture 2d, 4d and air drying 5d, 10d, 15d reached utmost point significant difference without significant difference in 2 years with ambient temperature and moisture 6d, 8d, air drying 1 year, low temperature drying; From average solid seed number: contrast and ambient temperature and moisture 2d, 4d and air drying 5d, 10d, 15d reached utmost point significant difference without significant difference in 2 years with ambient temperature and moisture 6d, 8d and low temperature drying.Presentation of results flue-cured tobacco pollen is easily stored, the ambient temperature and moisture condition can be preserved 4 days, air drying can be preserved 15 days at least, and the pollen that air drying was stored 1 year still has stronger vigor, can solve the difficulty that male parent early blossoming in the hybrid seeding or awful weather can't be pollinated and be brought to the production of hybrid seeds fully.
(3.3) different full-bloom stages are on the impact of hybrid seeding
Fine, air humidity is lower, and temperature is suitably high, and intensity of photosynthesis is conducive to greatly the synthetic and transportation of seed nutrient, all is favourable to improving seed production and quality.
(3.3.1) different full-bloom stage pollinations are on the impact of hybrid seed yield
The solid situation of the hybrid seeding of the different full-bloom stages of table 4
Found out by table 4: full-bloom stage late July than the hybrid seeding of early September solid good, thousand kernel weight is large, both reach utmost point significant difference and significant difference.Illustrate that the weather condition of late July is beneficial to the raising of hybrid seeding seed production and quality.
(3.3.2) different full-bloom stage pollinations are on the impact of seed sprouting power
Seed sprouting power refers to that seed germinates under optimum conditions and grows up to the ability of normal plant, represents with seed vitality certain correlation is arranged usually with germination vigor and germination rate.
Table 5 is on the impact of seed vigor
Found out by table 5: late July is higher than an early September pollination germination vigor that bears seeds and germination rate, and both reach significant difference.
By above different full-bloom stages on the impact of hybrid seeding efficient and seed quality and the results of analysis of variance as can be known, should note regulating the Parent date of seeding during hybrid seeding, full-bloom stage be met in late July, to improve hybrid seeding ripening rate and seed quality.
(3.4) difference stay the fruit several impacts on the flue-cured tobacco hybrid seeding
Stay the fruit number to affect seed production and quality by the balance that affects the source, storehouse, source foot, storehouse are conducive to greatly formation and the growth of seed.Nutritional condition and leaf area have determined the size in source, and therefore, the size in storehouse also should have certain restriction, surpass certain limit when individual plant stays the fruit number, and particularly the raising of seed production efficiency is unfavorable on the contrary to seed production and quality.
(3.4.1) difference is stayed the several impacts on hybrid seed yield and seed production efficiency of fruit
Choosing pollination stays the different individual plant of fruit number to compare result such as table 6.
Table 6 difference is stayed the several impacts on seed production efficiency of fruit
Learnt by table 6, stay the fruit number be 60,80,100 the fruit 3 processing between the average result rate without significant difference, and stay the fruit number be 120 the fruit, 200 the fruit processing between there were significant differences or utmost point significant difference; Staying the fruit number is that average solid seed number is without significant difference between the processing of 60 fruits, 80 fruits, and to stay the fruit number be that there were significant differences or utmost point significant difference between 100 fruits, 120 fruits, 200 fruits were processed.Illustrate that flue-cured tobacco production of hybrid seeds individual plant stays the fruit number should not surpass 80 fruits, consider from seed production and seed production efficiency, individual plant stays the fruit number the most suitable with 80 fruits.
(3.4.2) difference is stayed the several impacts on the hybrid seeding seed quality of fruit
The seed quality that choosing pollinates stays the different individual plant of fruit number to obtain compares result such as table 7.
Table 7 difference is stayed the several impacts on seed quality of fruit
Learnt by table 7, staying the fruit number is that thousand grain weigth is without significant difference between the processing of 60 fruits, 80 fruits, and to stay the fruit number be that 100 fruits, 120 fruits, 200 fruits have utmost point significant difference between processing; Stay the fruit number be less than or equal 100 the fruit 3 processing between percentage of seedgermination without significant difference, and stay the fruit number be 120 the fruit, 200 the fruit processing between there were significant differences or utmost point significant difference.Explanation considers from seed quality, and it also is the most suitable with 80 fruits that flue-cured tobacco production of hybrid seeds individual plant stays the fruit number.
Stay really several impacts on hybrid seeding efficient and seed quality and the results of analysis of variance as can be known by above difference, flue-cured tobacco male sterile hybrid seeding individual plant stays the fruit number to stay 80 fruits to be advisable with every strain.
(3.5) writing brush method pollinating method and seed production efficiency
Carrying out above test and producing in a large number in the process of crossbreed Guiyan 4, pollination compares to writing brush method and tweezers semar technique for we, the result show with the writing brush method than save time with the tweezers method, the solid situation of the production of hybrid seeds is good, seed production efficiency is high.The writing brush pollinating process is: gather the flower pesticide that second day will split every afternoon and be stored in laboratory, treat the next morning, flower pesticide splits, takes the field and gets pollen and be applied on the column cap that will pollinate with writing brush is sticking, namely finishes production of hybrid seeds process.
Adopt the writing brush method at the enterprising pedestrian worker's supplementary pollination of MS tobacco plant, 80 flowers of every strain pollination, fruiting rate is 95%, 140 milligrams of the average sowings of every fruit can be received 10 gram seeds (selected) on the strain MS plant.In addition, gather pollen and hybridization required time, each strain only needs about 10 minutes.Therefore, calculate by work in 8 hours, but for each person every day production of hybrid seeds 400 ~ 500 grams are equivalent to 200～300 mu of land for growing field crops sowing quantities, its production of hybrid seeds benefit is apparent.
(3.6) seeding technique effect
Take the statistical data of Chinese tobacco Jinsha breeding base in 2004 breeding " Guiyan 4 " as example: maternal 80 mu of plantation in 2004,5 mu of male parents, altogether results " Guiyan 4 " hybrid seed 428kg.Amount to crossbreed per mu yield 5.35kg, every worker produces 240 gram crossbreed.By 1.85 yuan of calculating of the every gram of crossbreed, compare with original production of hybrid seeds per mu yield, most effective value (seed per mu yield 2kg, every worker seeding every day 100g), 6917 yuan of every mu of increase of production of hybrid seeds output values, every worker increases by 259 yuan of the output values.Be total to 290 mu of the production of hybrid seeds in 6 years, average yield per mu 5.22kg, the newly-increased production of hybrid seeds of accumulative total is taken in 172.75 ten thousand yuan.
(4) analysis is discussed
The factor that affects the flue-cured tobacco hybrid seeding has: 1, column cap vigor; 2, the vigor of storage pollen; 3, full-bloom stage; 4, individual plant stays the fruit number; 5, pollinating method and pollination efficiency.
The impact of the column cap vigor is subjected to the column cap shape, the flowers are in blossom fate.The column cap shape is energetic with the column cap of 2 row, 3 row shapes, and front 1 day so that the flowers are in blossom column cap rear 1 day to the flowers are in blossom of the flowers are in blossom fate is energetic.
Flue-cured tobacco pollen ambient temperature and moisture can be preserved 4 days at least, and air drying can be preserved 15 days at least, and air drying is preserved and to be still had stronger vigor in 1 year, can solve the difficulty that the male parent early blossoming that often runs in the hybrid seeding and awful weather bring to pollination fully.
Full-bloom stage affects column cap vigor and Pollen Activity by the change of the weather conditions such as temperature, humidity, illumination, and then it is solid to affect hybrid seeding.Therefore entering full-bloom stage with late July is conducive to improve cross-fertile rate and hybrid quality.
Adopt the pollination of writing brush method, can produce for each person every day 200 ~ 300 gram seeds, seed production efficiency is high, benefit is large.
In result of the test, the solid situation between we find to make up is variant, but the factor of each combined parent strain column cap vigor and Pollen Activity of affecting is identical, and variation tendency is consistent.Therefore, when a certain particular combinations is carried out hybrid seeding, be noted that 1, regulate the kind date of seeding, the Parent full-bloom stage was met in late July.2, adopt the pollination of writing brush method, improve pollination efficiency.3, select 2 strong row of vitality and the pollination of 3 colonnade heads as far as possible, improve the solid seed number of every fruit.4, individual plant stays the fruit number should not surpass 80 fruits.5, run into special circumstances, in the time of preserving pollen, determine preservation condition depending on the fate that needs to preserve, be strictly on guard against that pollen viability descends.
Owing to having adopted technique scheme, compared with prior art, the present invention gets powder time and method to planting patterns, planting proportion, florescence, the male parent of Parent, and pollination time and method and maternal individual plant stay link such as fruit number etc. to improve, to improve efficient and the quality of the tobacco hybridization production of hybrid seeds.Reduced the cultivated area of tobacco male parent, reduced the hybrid seeding artificial supplementary pollination amount of labour used, improved pollination efficiency, improved hybrid seed yield, reduced the production cost of hybrid seeding, had good economic and social profit, promotion prospect is wide.The present invention easily implements, and result of use is good.
Embodiment
Embodiments of the invention 1: hybrid seed production method for tobacco, with the male parent of tobacco and maternal separately plantation, male parent is 1:15 with maternal planting proportion, and regulates date of seeding, makes male parent than maternal prematurity about 15 days, makes the full-bloom stage of maternal plant be in late July; Behind the male parent initial bloom stage, before 10 of every days, take off the flower that to open the same day, at indoor concentrated taking-up flower pesticide, thickness with 1～2cm is tiled in the culture dish, then be placed on make Anther dehiscence in the aeration cabinet of the local of aeration-drying or 34～36 ℃ after, remove anther wall with 80 purpose stainless steel sifts sieves, only stay pollen, be placed on again in the refrigerator that is kept at 2～4 ℃ in the drier storage for subsequent use; When maternal plant is in full-bloom stage, after 10 of fine mornings, select on the maternal plant that corolla is little redly to split to corolla, but here column cap does not wither, and column cap is that the flower of 2 row or 3 row is pollinated, the fruit of choosing the cigarette strain to tie is cut, only stay flower and bud, tear the corolla of having bloomed, expose column cap, very thin (prick carefully with adhesive tape or fine rule, main purpose is to prevent that nib from scattering in pollinating process with nib, and guarantee the contact-making surface of nib and the sizableness of maternal column cap, avoid causing the waste of paternal pollen) the drunk paternal pollen of writing brush evenly spread upon on the maternal column cap; Keeping the individual plant pollination to stay the fruit number is 80 fruits.
Embodiments of the invention 2: hybrid seed production method for tobacco, with the male parent of tobacco and maternal separately plantation, male parent is 1:10 with maternal planting proportion, and regulates date of seeding, makes male parent than maternal prematurity about 20 days, makes the full-bloom stage of maternal plant be in early September; Behind the male parent initial bloom stage, before 10 of every days, take off the flower that to open the same day, at indoor concentrated taking-up flower pesticide, thickness with 1～2cm is tiled in the culture dish, then be placed on make Anther dehiscence in the aeration cabinet of the local of aeration-drying or 34～36 ℃ after, remove anther wall with 50 purpose stainless steel sifts sieves, only stay pollen, be placed on again in the refrigerator that is kept at 2～4 ℃ in the drier storage for subsequent use; When maternal plant is in full-bloom stage, after 10 of fine mornings, select on the maternal plant that corolla is little redly to split to corolla, but here column cap does not wither, and column cap be 2 row or 3 row flowers pollinate, the fruit of choosing the cigarette strain to tie is cut, only stay flower and bud, tear the corolla of having bloomed, expose column cap, evenly spread upon on the maternal column cap with the drunk paternal pollen of the very thin writing brush of nib; Keeping the individual plant pollination to stay the fruit number is 80 fruits.
Embodiment 3: hybrid seed production method for tobacco, and with the male parent of tobacco and maternal separately plantation, male parent is 1:20 with maternal planting proportion, and regulates date of seeding, makes male parent than maternal prematurity about 10 days, makes the full-bloom stage of maternal plant be in late July; Behind the male parent initial bloom stage, before 10 of every days, take off the flower that to open the same day, at indoor concentrated taking-up flower pesticide, thickness with 1～2cm is tiled in the culture dish, then be placed on make Anther dehiscence in the aeration cabinet of the local of aeration-drying or 34～36 ℃ after, remove anther wall with 100 purpose stainless steel sifts sieves, only stay pollen, be placed on again in the refrigerator that is kept at 2～4 ℃ in the drier storage for subsequent use; When maternal plant is in full-bloom stage, after 10 of fine mornings, select on the maternal plant that corolla is little redly to split to corolla, all flowers here are not pollinated but column cap withers, the fruit of choosing the cigarette strain to tie is cut, only stay flower and bud, tear the corolla of having bloomed, expose column cap, evenly spread upon on the maternal column cap with the drunk paternal pollen of the very thin writing brush of nib; Keeping the individual plant pollination to stay the fruit number is 100 fruits.
Claims (4)
1. hybrid seed production method for tobacco is characterized in that: with male parent and the maternal separately plantation of tobacco, and regulate the florescence with date of seeding, make male parent than maternal prematurity 10～20 days; Behind the male parent initial bloom stage, take off the flower that flower pesticide does not split every day, collect pollen storage for subsequent use; When maternal plant is in full-bloom stage, the fruit of choosing the cigarette strain to tie is cut, only stay flower and bud, tear the corolla of having bloomed, expose column cap, paternal pollen is evenly spread upon on the maternal column cap; Keeping the individual plant pollination to stay the fruit number is 80～100 fruits; Male parent is 1:10～20 with the planting proportion of female parent; Behind the male parent initial bloom stage, before 10 of every days, take off the flower that to open the same day, at indoor concentrated taking-up flower pesticide, thickness with 1～2cm is tiled in the culture dish, then be placed on make Anther dehiscence in the aeration cabinet of the local of aeration-drying or 34～36 ℃ after, remove anther wall with 50～100 purpose stainless steel sifts sieves, only stay pollen, be placed on again in the refrigerator that is kept at 2～4 ℃ in the drier storage for subsequent use.
2. hybrid seed production method for tobacco according to claim 1 is characterized in that: at maternal full-bloom stage, after 10 of fine mornings, select on the maternal plant that corolla is little redly to split to corolla, and column cap is that the flowers of 2 row or 3 row are pollinated.
3. hybrid seed production method for tobacco according to claim 1 is characterized in that: when pollinating, adopt the writing brush semar technique to pollinate, dip in paternal pollen with writing brush and evenly spread upon on the maternal column cap.
4. hybrid seed production method for tobacco according to claim 1 is characterized in that: make the full-bloom stage of maternal plant be in late July.
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| CN115918516B (en) * | 2022-10-31 | 2023-09-26 | 玉溪中烟种子有限责任公司 | Method for reducing pistil malformation rate and propagating and planting tobacco sua cytoplasmic male sterile line |
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Non-Patent Citations (4)
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| 刘仁祥等.烤烟雄性不育杂交种制种技术研究.《中国烟草科学》.2000,(第4期),摘要,第6页第2栏第21行-第7页第1栏第7行,第8页第1栏第3-7行,第10页第1栏第11行-第2栏第5行. * |
| 杨全柳等.烤烟雄性不育杂交种制种技术研究.《中国烟草科学》.2006,(第2期),29-31. * |
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