CN102095722A - Method for measuring content of phosphorus in plant - Google Patents
Method for measuring content of phosphorus in plant Download PDFInfo
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- CN102095722A CN102095722A CN2009102167554A CN200910216755A CN102095722A CN 102095722 A CN102095722 A CN 102095722A CN 2009102167554 A CN2009102167554 A CN 2009102167554A CN 200910216755 A CN200910216755 A CN 200910216755A CN 102095722 A CN102095722 A CN 102095722A
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Abstract
The invention discloses a method for measuring the content of phosphorus in a plant, which comprises the following steps of: (1) putting a dried and ground plant sample into a conical flask, dripping a water-wet sample, and adding concentrated sulfuric acid; (2) shaking up solution, putting a funnel at the opening of the flask, and digesting over slow fire; (3) raising the temperature after H2SO4 is decomposed and white smoke is discharged, and taking down and cooling when the solution is brown black; (4) dripping H2O2, shaking up, heating until the solution is boiled, digesting, cooling, repeatedly dripping the H2O2, digesting, repeating until the solution is colorless or clear, and heating to completely remove residual H2O2; (5) taking the conical flask down, cooling the solution, washing the funnel by using water, making washing solution flow into the conical flask, washing digestion solution, putting the washed digestion solution into another volumetric flask, fixing the volume, shaking up, and filtering or clarifying; and (6) performing color development on the filtered or clarified solution, and performing color comparison to determine the content of the phosphorus. The method is simple and has high measuring accuracy.
Description
Technical field
The present invention relates to the assay method of plant phosphorus content.
Background technology
Phosphorus is one of indispensable a great number of elements of growth and development of plants, is the important composition composition of plant, and simultaneously various physiological and biochemical procedures in the involved in plant body in many ways again play an important role to promoting growth and development of plant and metabolism.China ploughs 100,000,000 hectares approximately, accounts for about 6,667 ten thousand hectares with lacking phosphorus.The phosphorus element that crop utilized is mainly derived from soil, and soil lacks phosphorus (" science of heredity lacks phosphorus ") has become one of most important restriction factor in the agricultural production
[33]Corn is not only important crops, and is the good feed of developing animal husbandry and the important raw material of industry.
For same plant, the significant difference that different cultivars or product tie up to phosphorus absorption, transhipment and utilization ratio aspect all has report on crops such as wheat, barley, paddy rice, corn, Chinese sorghum, Kidney bean, cotton, Chinese milk vetch.Fageria and Baligai have studied the difference of the phosphate use efficient (promptly being accumulated in the dry that every milligram of phosphorus produced in the plant) of 15 wheat genotypes.The result shows that there is the difference of highly significant in the phosphate use efficient of 15 wheat genotypes.Li Jiyun etc. studies show that by field test phosphorus absorption, phosphate use efficient and the grain yield of six wheat genotypes all are significant genotypic difference.Liu Xiangsheng etc. have studied the reaction of 100 parts of corn inbred lines to low-phosphorus stress by the phosphate fertilizer long term experiment.The result shows that the output of different corn inbred lines is significant difference.Wang Rongping etc. have studied the yield traits of low-phosphorous processing and two kinds of following 21 corn inbred lines of condition of high phosphorus processing.The result shows that the output of 21 corn inbred lines all is significant genotypic difference under two kinds of phosphorus treatment conditions, and low-phosphorus stress has strengthened the difference degree of different genotype corn yield.Employing pot experiments such as Zhang Limei have been studied the phosphorus nutrition characteristic of five corn inbred lines of different anti-low-phosphorous characteristics in different growing stages.The result shows, corn seedling stage and jointing stage, under the low-phosphorus stress, the relative phosphorus amount of inhaling with 99239 plant of anti-low-phosphorous self-mating system 99180 all is significantly higher than responsive self-mating system 99152.In boot stage, it is higher that the anti-low-phosphorous characteristic of corn inbred line is mainly reflected in upper leaf phosphorus semi-invariant; Weave silk the phase, though anti-low-phosphorous self-mating system 99180 and 99239 under low-phosphorus stress, the phosphorus absorption efficiency and the reallocation efficient still apparently higher than responsive self-mating system 99152.The result of study of Furlani etc. shows that under low-phosphorous treatment conditions, all there is significant genotypic difference in different sorghum varieties aspect absorption, utilization and the allocative efficiency of phosphorus.The plain utilization ratio of phosphorus efficiency genotype dry matter production, phosphorus accumulation and phosphorus all is significantly higher than phosphorus poor efficiency genotype.The child learns military affairs etc. and studies show that under low-phosphorous hydroponics, the ability of titanium pigment there are differences in the different soybean genotype absorbent solutions, and the phosphorus amount of absorption can reach about the 5%-80% of self intrinsic phosphorus amount.
Therefore, it is quite important that the mensuration of plant phosphorus content seems, has only the monitoring of long-term assurance plant phosphorus content could in time carry out the adjustment of phosphorus element content to plant.
Summary of the invention
The object of the present invention is to provide a kind of plant phosphorus Determination on content method, when simplifying assay method, improve and measure accuracy rate.
To achieve these goals, the technical solution used in the present invention is as follows;
Plant phosphorus Determination on content method is characterized in that, may further comprise the steps:
(1) plant sample that get oven dry, grinds places in the triangular flask, splashes into the moistening sample of water, adds the concentrated sulphuric acid then;
(2) shake up solution in the triangular flask, bottleneck is put a funnel, and boiling on the stove disappears with slow fire boils disappearing;
(3) work as H
2SO
4Decompose and to emit the Bai Yanhou temperature that raises again, when solution is even brownish black, take off cooling;
(4) drip H
2O
2, shake up and be heated to boiling again and disappear again and boil, take off cooling then, repeat to drip H
2O
2, disappear once more and boil, so repeat up to solution be colourless or limpid after, heat again to eliminate remaining H
2O
2
(5) take off the triangular flask cooling solution, and water flushing funnel, washing lotion flows into triangular flask, will disappear to boil harmless the washing in another volumetric flask of liquid, and the water constant volume shakes up, and filters or place clarification;
(6) with the anti-developer of molybdenum antimony the solution after filtering or clarifying is developed the color, colorimetric on photometer is judged phosphorus content according to color then.
Time of boiling that at every turn disappears in the described step (4) is 5 minutes.
Disappearing in the described step (4), to boil number of times be 3～5 times.
It is 10 minutes that the middle solution of described step (4) is colourless or limpid back heat time heating time.
Assay method of the present invention is very simple, only adopts common surveying instrument to measure under conventional environment, and environment without any specific (special) requirements, is not affected by the external environment yet, and it is very high therefore to measure accuracy rate.
Embodiment
The invention will be further described below by coming for example.
Assay method of the present invention may further comprise the steps;
One. make solution to be measured
At first take by weighing oven dry, about the plant sample that grinds (root, stem and leaf) 0.2g.Place the 50ml triangular flask.Splash into the moistening sample of less water earlier, add the 8ml concentrated sulphuric acid then, shake up gently, bottleneck is put a little funnel, boils on the stove first slow fire and disappears and boil disappearing, and treats H
2SO
4Decompose and to emit the temperature that raises again behind a large amount of white cigarettes, when solution is even brownish black, take off, add 10 H after cold slightly
2O
2, shake up and be heated to boiling again, disappear and boil about 5 minutes, take off, cold slightly after, repeat to add 5～10 H
2O
2, disappear once more and boil.So repeat totally 3～5 times, add H at every turn
2O
2Amount should reduce at every turn, disappear boil solution be colourless or limpid after, again the heating about 5-10min to eliminate remaining H
2O
2(otherwise can influence N, P colorimetric estimation).Take off cooling.Answer low amounts of water to wash little funnel, washing lotion flows into triangular flask.To disappear and boil harmless the washing in the 100ml volumetric flask of liquid, the water constant volume shakes up.Filter or place the mensuration of clarification back phosphorus supply.
Two. transfer pH and colorimetric
Draw filtrate 5ml in the 50ml volumetric flask, thin up adds 2 of dinitrophenol dinitrophenolate indicator to 30ml, with sodium hydroxide solution [c (NaOH)=0.2mol.L
-1] and dilution heat of sulfuric acid [c (H
2SO
4)=0.5mol.L
-1] transfer PH just to be little yellow to solution.Add the anti-developer 5ml of molybdenum antimony then, shake up the water constant volume.Place 30 minutes under room temperature is higher than 15 ℃ after, with wavelength 700nm colorimetric, be the reference liquid zeroing with blank test solution on spectrophotometer, read absorption value, it is stable that color can keep in 8 hours.
Assay method of the present invention and process are very simple, and the mensuration cycle is short, not only simplified the mensuration process greatly, and cost of determination is low, the accuracy rate height.
Claims (4)
1. plant phosphorus Determination on content method is characterized in that, may further comprise the steps:
(1) plant sample that get oven dry, grinds places in the triangular flask, splashes into the moistening sample of water, adds the concentrated sulphuric acid then;
(2) shake up solution in the triangular flask, bottleneck is put a funnel, and boiling on the stove disappears with slow fire boils disappearing;
(3) work as H
2SO
4Decompose and to emit the Bai Yanhou temperature that raises again, when solution is even brownish black, take off cooling;
(4) drip H
2O
2, shake up and be heated to boiling again and disappear again and boil, take off cooling then, repeat to drip H
2O
2, disappear once more and boil, so repeat up to solution be colourless or limpid after, heat again to eliminate remaining H
2O
2
(5) take off the triangular flask cooling solution, and water flushing funnel, washing lotion flows into triangular flask, will disappear to boil harmless the washing in another volumetric flask of liquid, and the water constant volume shakes up, and filters or place clarification;
(6) with the anti-developer of molybdenum antimony the solution after filtering or clarifying is developed the color, colorimetric on photometer is judged phosphorus content according to color then.
2. plant phosphorus Determination on content method according to claim 1 is characterized in that, the time of boiling that at every turn disappears in the described step (4) is 5 minutes.
3. plant phosphorus Determination on content method according to claim 1 is characterized in that, disappearing in the described step (4), to boil number of times be 3～5 times.
4. plant phosphorus Determination on content method according to claim 1 is characterized in that, it is 10 minutes that the middle solution of described step (4) is colourless or limpid back heat time heating time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102167554A CN102095722A (en) | 2009-12-14 | 2009-12-14 | Method for measuring content of phosphorus in plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102167554A CN102095722A (en) | 2009-12-14 | 2009-12-14 | Method for measuring content of phosphorus in plant |
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| Publication Number | Publication Date |
|---|---|
| CN102095722A true CN102095722A (en) | 2011-06-15 |
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ID=44128904
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009102167554A Pending CN102095722A (en) | 2009-12-14 | 2009-12-14 | Method for measuring content of phosphorus in plant |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103837426A (en) * | 2014-02-28 | 2014-06-04 | 西北农林科技大学 | Method for detecting silage mildew |
| CN104165849A (en) * | 2014-08-01 | 2014-11-26 | 湖北富邦科技股份有限公司 | Method for determining total phosphorous in surfactant |
| CN104458593A (en) * | 2014-12-10 | 2015-03-25 | 甘肃省林业科学研究院 | Method for nutrient diagnosis of olive leaves |
| CN111089861A (en) * | 2020-01-20 | 2020-05-01 | 甘肃省农业科学院经济作物与啤酒原料研究所（甘肃省农业科学院中药材研究所） | Method for determining nitrogen, phosphorus and potassium contents of barley plants |
| CN113984754A (en) * | 2021-12-02 | 2022-01-28 | 云南省农业科学院农业环境资源研究所 | Continuous detection device and method for nitrogen and phosphorus in organic fertilizer |
-
2009
- 2009-12-14 CN CN2009102167554A patent/CN102095722A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103837426A (en) * | 2014-02-28 | 2014-06-04 | 西北农林科技大学 | Method for detecting silage mildew |
| CN104165849A (en) * | 2014-08-01 | 2014-11-26 | 湖北富邦科技股份有限公司 | Method for determining total phosphorous in surfactant |
| CN104458593A (en) * | 2014-12-10 | 2015-03-25 | 甘肃省林业科学研究院 | Method for nutrient diagnosis of olive leaves |
| CN111089861A (en) * | 2020-01-20 | 2020-05-01 | 甘肃省农业科学院经济作物与啤酒原料研究所（甘肃省农业科学院中药材研究所） | Method for determining nitrogen, phosphorus and potassium contents of barley plants |
| CN113984754A (en) * | 2021-12-02 | 2022-01-28 | 云南省农业科学院农业环境资源研究所 | Continuous detection device and method for nitrogen and phosphorus in organic fertilizer |
| CN113984754B (en) * | 2021-12-02 | 2024-04-16 | 云南省农业科学院农业环境资源研究所 | Device and method for continuously detecting nitrogen and phosphorus in organic fertilizer |
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Application publication date: 20110615 |