CN102093576B - Preparation method of bionic soft tissue material-polyvinyl alcohol hydrogel - Google Patents
Preparation method of bionic soft tissue material-polyvinyl alcohol hydrogel Download PDFInfo
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- CN102093576B CN102093576B CN201010616735A CN201010616735A CN102093576B CN 102093576 B CN102093576 B CN 102093576B CN 201010616735 A CN201010616735 A CN 201010616735A CN 201010616735 A CN201010616735 A CN 201010616735A CN 102093576 B CN102093576 B CN 102093576B
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Abstract
A preparation method of bionic soft tissue material-polyvinyl alcohol hydrogel comprises the following steps: 1) weighting 10-20g of flocculent polyvinyl alcohol; 2) weighting 90-180g of deionized water; 3) placing polyvinyl alcohol a 95 DEG C of thermostatic stirrer to fully dissolve and obtain 100-200g of 10% polyvinyl alcohol solution; cooling to 40 DEG C, adding 10-20g of epichlorohydrin and 8-10g of potassium hydroxide solution in turn, stirring for fully mixing; 4) cooling the mixed solution to 30 DEG, adding 20-30g of dimethyl sulfoxide, mixing evenly, fasting pouring the solution in a mould; 5) placing the mould in a freezer, freezing at -20 DEG C for 24 hours, taking the mould out to unfreeze at the room temperature for 24 hours; 6) and repeating the step 5) for certain times to obtain composite hydrogel. The method of the invention is simple to operate and easy to realize; and the parameters can be adjusted according to the characteristics of different tissues and the hydrogel is easy to obtain. The bionic soft tissue material prepared by the method has stable properties, is easy to store and can be recycled.
Description
Technical field
The present invention relates to the preparation method of a kind of bionical thing soft-tissue material.Particularly relate to a kind of similarly, can be used for bionical thing soft-tissue material-polyvinyl alcohol hydrogel preparation method that particle is implanted experiment with the pig liver material mechanical performance.
Background technology
The most frequently used material of the research bionical thing soft tissue surrogate of preparation is a macromolecule hydrogel both at home and abroad.Macromolecule hydrogel is made up of cancellated superpolymer and water, has very strong water-absorbent, swelling property, thixotropy, dewatering shrinkage, elasticity and biocompatibility.Therefore these characteristics make its surrogate that can do the human body soft tissue, cause that also many scientific workers pay attention to widely and study.The characteristic of research hydrogel makes people recognize structure and performance that organizing of the many organisms of nature all has the similar water gel, and this just inspires people that the hydrogel macromolecular material is used as biomaterial for medical purpose.Present medical aquogel through chemosynthesis; In release vehicle, enzyme, antigen, the separation of antibody of the cultivation of biomass cells, protease activities control, medicine, human body soft tissue is filled and all respects such as organizational project are applied, and aspect intelligent material, has the potential using value.
Because the PVA hydrogel is except the performance that possesses general hydrogel; Have especially that toxicity is low, mechanical property is good (high elastic coefficient and high physical strength), advantage such as water regain height and good biocompatibility; Thereby gain great popularity, become the maximum water-soluble polymers of output in the world at present.The purposes of PVA hydrogel aspect biomedical and experiment is very extensive, can be used as artificial skin, artificial surrogate such as artificial muscle.
Summary of the invention
Technical problem to be solved by this invention is, provides a kind of soft tissue that can substitute to carry out bionical thing soft-tissue material-polyvinyl alcohol hydrogel preparation method that particle is implanted experiment.
The technical scheme that the present invention adopted is: a kind of bionical thing soft-tissue material-polyvinyl alcohol hydrogel preparation method comprises the steps:
1) takes by weighing cotton-shaped Z 150PH 10-20g by 1: 9 weight proportion; Measure deionized water 90-180g;
2) be placed into dissolving fully in 95 ℃ the thermostatic mixer, obtain 10% polyvinyl alcohol water solution 100-200g;
3) be cooled to 40 ℃, add epoxy chloropropane 10-20g and potassium hydroxide aqueous solution 8-10g successively, be stirred to thorough mixing;
4) mixing solutions is cooled to 30 ℃, adds DMSO 99.8MIN. 20-30g, after mixing, pour in the mould rapidly;
5) mould is put into refrigerator-freezer,, take out and under normal temperature, thawed 24 hours at-20 ℃ after freezing 24 hours;
6), thereby obtain composite aquogel by the number of times repeating step of setting 5;
Adopt step 1-6 to prepare corresponding 12 groups of transparent polyethylenes alcohol hydrogel,, described 12 groups of transparent polyethylenes alcohol hydrogel is divided into 4 organizes greatly according to the multiplicity of step 6; Proportioning according to each material in every group; Be divided into 3 groups again, carry out stretching experiment to 12 groups of hydrogels that make respectively by group, the mean stress of drawing curve; And do stress and strain contrast with the liver stress strain curve, obtain the best proportioning and the freeze-thaw cycle index of each composition of polyvinyl alcohol hydrogel.
Bionical thing of the present invention soft-tissue material-polyvinyl alcohol hydrogel preparation method; Method is simple to operate; Be easy to realize, and can obtain easily according to the characteristic adjusting parameter of different tissues; Overcome the problem that biological tissue is retrained by ethics, law etc., in clinical medicine and experiment, very application prospects has been arranged.The bionical thing soft-tissue material that adopts that this method prepares, stable in properties is easy to preserve, and can be recycled.
Description of drawings
Fig. 1 is 9 groups of liver samples stress-strain curves;
Fig. 2 is 9 groups of PVA sample stress-strain curves;
Fig. 3 is the stretching correlation curve of recirculated water gel and liver;
Fig. 4 is the stretching correlation curve of secondary circulation hydrogel and liver;
Fig. 5 is the stretching correlation curve of three recirculated water gels and liver;
Fig. 6 is the stretching correlation curve of four recirculated water gels and liver.
Embodiment
Below in conjunction with embodiment and accompanying drawing bionical thing of the present invention soft-tissue material-polyvinyl alcohol hydrogel preparation method is made detailed description.
Bionical thing of the present invention soft-tissue material-polyvinyl alcohol hydrogel preparation method comprises the steps:
1) takes by weighing cotton-shaped Z 150PH (PVA) 10-20g by 1: 9 weight proportion; Measure deionized water 90-180g;
2) be placed into dissolving fully in 95 ℃ the thermostatic mixer, obtain 10% polyvinyl alcohol water solution 100-200g, described polyvinyl alcohol water solution is the water white transparency shape, and viscosity is higher;
3) 10% polyvinyl alcohol water solution is cooled to 40 ℃, adds epoxy chloropropane 10-20g and potassium hydroxide aqueous solution 8-10g successively, be stirred to thorough mixing according to certain prescription;
4) mixing solutions is cooled to 30 ℃, adds DMSO 99.8MIN. 20-30g, after mixing, pour in the mould rapidly;
5) then mould is put into refrigerator-freezer,, take out and under normal temperature, thawed 24 hours at-20 ℃ after freezing 24 hours;
6), thereby obtain having certain intensity and elastic composite aquogel by the number of times repeating step of setting 5.
The hydrogel (material different is added proportioning and cold, thaw cycles number of times) that also includes preparation carries out stretching experiment, and compares with the liver organization stress strain curve, obtains the best proportioning and the freeze-thaw cycle index of each composition of hydrogel.
Be best proportioning and the cold thaw cycles number of times that obtains each composition of hydrogel, prepare corresponding 12 groups of transparent PVA hydrogels.Can be divided into 4 groups again by the cycle index difference, the proportioning of each material is different in every group, can be divided into 3 groups again.Carry out stretching experiment to 12 groups of hydrogels that make respectively according to the experimental technique of front, the mean stress of drawing curve, and do contrast with the liver curve.As shown in table 1:
12 groups of material mixture ratios of table 1 preparation transparent aquagel
Z 150PH (PVA) hydrogel to preparation carries out 1～4 round-robin test respectively, can know that through the stress strain curve of PVA stress strain curve and liver organization preferred forms of the present invention is: prepare 10% PVA aqueous solution 200g; Be cooled to 40 ℃, add epoxy chloropropane 14g successively, potassium hydroxide aqueous solution 8g; Be stirred to thorough mixing, again mixing solutions be cooled to 30 ℃, add DMSO 99.8MIN. 20g; After mixing, pour in the mould rapidly.Then mould is put into refrigerator-freezer, behind-20 ℃ of freezing 24h, take out the 24h that under normal temperature, thaws, repeat so cold, thaw cycles process 4 times on request, can obtain the tensile property composite aquogel similar with liver.Utilize this kind material to add proportioning preparation experiment sample, be used to simulate soft tissue, can be used as the experimental subjects in later stage.
Claims (1)
1. bionical thing soft-tissue material-polyvinyl alcohol hydrogel preparation method is characterized in that, comprises the steps:
1) takes by weighing cotton-shaped Z 150PH 10-20g by 1: 9 weight proportion; Measure deionized water 90-180g;
2) be placed into dissolving fully in 95 ℃ the thermostatic mixer, obtain 10% polyvinyl alcohol water solution 100-200g;
3) be cooled to 40 ℃, add epoxy chloropropane 10-20g and potassium hydroxide aqueous solution 8-10g successively, be stirred to thorough mixing;
4) mixing solutions is cooled to 30 ℃, adds DMSO 99.8MIN. 20-30g, after mixing, pour in the mould rapidly;
5) mould is put into refrigerator-freezer,, take out and under normal temperature, thawed 24 hours at-20 ℃ after freezing 24 hours;
6), thereby obtain composite aquogel by the number of times repeating step of setting 5;
Adopt step 1-6 to prepare corresponding 12 groups of transparent polyethylenes alcohol hydrogel,, described 12 groups of transparent polyethylenes alcohol hydrogel is divided into 4 organizes greatly according to the multiplicity of step 6; Proportioning according to each material in every group; Be divided into 3 groups again, carry out stretching experiment to 12 groups of hydrogels that make respectively by group, the mean stress of drawing curve; And do stress and strain contrast with the liver stress strain curve, obtain the best proportioning and the freeze-thaw cycle index of each composition of polyvinyl alcohol hydrogel.
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| CN201010616735A CN102093576B (en) | 2010-12-31 | 2010-12-31 | Preparation method of bionic soft tissue material-polyvinyl alcohol hydrogel |
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| CN102093576B true CN102093576B (en) | 2012-08-29 |
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| CN106823019A (en) * | 2016-12-21 | 2017-06-13 | 南京大学 | A kind of selfreparing ultrasonic puncture polyvinyl alcohol hydrogel biomimetic material and its preparation method and application |
| CN107374667A (en) * | 2017-06-30 | 2017-11-24 | 无锡海斯凯尔医学技术有限公司 | Body moulds Preparation Method |
| CN108310469B (en) * | 2018-01-25 | 2020-09-22 | 四川大学 | Preparation method of high-performance polyvinyl alcohol hydrogel artificial cartilage substitute material |
| CN109859600B (en) * | 2019-02-27 | 2021-06-01 | 北京大学第三医院（北京大学第三临床医学院） | Ultrasound-guided mammary gland tumor puncture model and preparation method thereof |
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| US20060205862A1 (en) * | 2003-04-15 | 2006-09-14 | Rolf Muller | Polyvinyl alcohol gels |
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| JP2005060467A (en) * | 2003-08-08 | 2005-03-10 | Denki Kagaku Kogyo Kk | Gel composition |
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| US20060205862A1 (en) * | 2003-04-15 | 2006-09-14 | Rolf Muller | Polyvinyl alcohol gels |
Non-Patent Citations (2)
| Title |
|---|
| 杨荣杰等.聚乙烯醇/二甲基亚砜/水凝胶的稳定性研究.《北京理工大学学报》.2009,第29卷(第2期),第164-167,171页. * |
| 江波等.化学交联PVA水凝胶中水的状态.《高分子材料科学与工程(1994年)》.1994,(第1期),第130-132页. * |
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