CN102091302A - Compound traditional Chinese medicine with anti-hepatocarcinoma effect and application thereof - Google Patents
Compound traditional Chinese medicine with anti-hepatocarcinoma effect and application thereof Download PDFInfo
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Abstract
The invention discloses a compound traditional Chinese medicine with an anti-hepatocarcinoma effect and an application thereof. The compound traditional Chinese medicine is prepared from 4-12 parts of wartwort, 4-12 parts of uniflower swisscentaury root, 4-12 parts of rhizoma zedoariae, 5-15 parts of astragalus root and 4-12 parts of donkey-hide glue. In the compound traditional Chinese medicine with an anti-hepatocarcinoma effect provided by the invention, raw materials are selected, matched and prescribed based on the traditional Chinese medicine theory and the pathogenesis of hepatocarcinoma, and the proportioning of all components is scientific and reasonable. Shown by pharmacological experiments and clinical experimental results, the compound traditional Chinese medicine disclosed by the invention has a good anti-hepatocarcinoma effect, not only can obviously improve the subjective symptoms of patients, increase the survival rate, improve the biochemical indicators and reduce the focus range, but also is convenient to take clinically, has no adverse reaction in long-term use, and can be developed into a new-generation anti-hepatocarcinoma medicament hopefully.
Description
Technical field
The present invention relates to a kind of Chinese medicine compound, be specifically related to a kind of Chinese medicine compound and application in preparation treatment liver-cancer medicine thereof, the invention still further relates to polysaccharide that extraction obtains from Chinese medicine compound and its production and application with antihepatocarcinoma effect.
Background technology: statistics shows that sickness rate accounts for the hepatocarcinoma of all tumor diseases 5%, and the annual whole world has 260,000 new cases approximately, but 5 years survival rate less thaies 5%.As the China of one of hepatocarcinoma state occurred frequently, the PLC mortality rate is over nearly 20 years even in rising trend.The characteristics that hepatocarcinoma has the onset concealment, progress is rapid, prognosis is relatively poor, life cycle is short, present main treatment means as: under the utilization of methods such as excision, radiotherapy, chemotherapy, intervention, therapeutic effect is very limited, and the expense costliness, and patient's ability to shoulder economically is poor.In addition, side effect that chemicotherapy causes also is the bottleneck of clinical treatment, and as one of important means of hepatocarcinoma Comprehensive Treatment, Chinese medicine is being brought into play the effect that can not be substituted because of its significant clinical efficacy in many treatment for cancer such as hepatocarcinoma.Therefore necessary under the guidance of traditional Chinese medicine theory, it is good to research and develop out a kind of anti-liver cancer efficacy, and untoward reaction is low, reasonable price, the compound Chinese medicinal preparation of easily applying.
Summary of the invention: goal of the invention: technical problem to be solved by this invention is to overcome the deficiencies in the prior art, provide a kind of anti-liver cancer efficacy good, side effect is little, the Chinese medicine compound with antihepatocarcinoma effect of taking convenience, and another object of the present invention provides from this Chinese medicine compound and extracts polysaccharide of obtaining and preparation method thereof and its application preparation treatment liver-cancer medicine.
Technical scheme: in order to realize above purpose, the Chinese medicine compound with antihepatocarcinoma effect provided by the invention, it is to be made by the raw material of following parts by weight: 4～12 parts of Herba Euphorbiae Helioscopiae, 4～12 parts of Radix Rhapontici, 4～12 parts of Rhizoma Curcumae, 5～15 parts of the Radixs Astragali, 4～12 parts in Colla Corii Asini.
As preferred version, above-described Chinese medicine compound with antihepatocarcinoma effect, it is to be made by the raw material of following parts by weight: 6～10 parts of Herba Euphorbiae Helioscopiae, 6～10 parts of Radix Rhapontici, 6～10 parts of Rhizoma Curcumae, 8～12 parts of the Radixs Astragali, 6～10 parts in Colla Corii Asini.
As preferred version, above-described Chinese medicine compound with antihepatocarcinoma effect, it is to be made by the raw material of following parts by weight: 6 parts of Herba Euphorbiae Helioscopiae, 6 parts of Radix Rhapontici, 6 parts of Rhizoma Curcumae, 8 parts of the Radixs Astragali, 6 parts in Colla Corii Asini.
The present invention is under the guidance of Chinese medical theory, determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs, pathogeny and disease symptom in conjunction with hepatocarcinoma, anticancer with anticancer detoxifcation, clearing away heat-damp and promoting diuresis, regulating the flow of QI to dissipate blood stasis, resolving phlegm and softening hard masses method, with benefiting vital QI and blood, the treatment rule that the supplementing QI and nourishing YIN method is set upright is screened prescription, through lot of experiments and clinical practice, selected Radix Rhapontici, Herba Euphorbiae Helioscopiae, Rhizoma Curcumae, the Radix Astragali, Colla Corii Asini Chinese medicine of the five flavours carry out prescription, Herba Euphorbiae Helioscopiae is the dry herb of dicotyledon Euphorbiaceae Euphorbiaceae Herba Euphorbiae Helioscopiae Euphorbia helioscopia L. in the side, cold nature, bitter in the mouth; Return large intestine, small intestinal, lung meridian, have inducing diuresis to remove edema, dissipating phlegm and resolving masses, the effect of killing parasites for relieving itching; Radix Rhapontici is the feverfew centaurea monanthos Georgi in the side. or the root of Radix Echinopsis, bitter in the mouth is salty, and is cold, has heat-clearing and toxic substances removing, the effect that eliminating carbuncle, Shujin are promoted blood circulation; Rhizoma Curcumae acrid in the mouth, hardship, temperature is returned liver, spleen channel, has removing blood stasis promoting the circulation of QI to relieve pain, the pain relieving of circulation of qi promoting removing food stagnancy, removing blood stasis analgesic effect; Radix Astragali nature and flavor are sweet, tepor, return through: return lung, spleen, liver, kidney channel, have the effect that benefiting QI for strengthening the superficies, arresting sweating take off, hold in the palm skin ulcer granulation promoting, inducing diuresis to remove edema admittedly; The Colla Corii Asini sweet in the mouth, flat, return lung, liver, kidney channel, have and enrich blood, hemostasis, nourshing Yin and drynsessmoistening prescription effect.Radix Rhapontici, Herba Euphorbiae Helioscopiae and Rhizoma Curcumae have the effect of anticancer detoxifcation, clearing away heat-damp and promoting diuresis, regulating the flow of QI to dissipate blood stasis, resolving phlegm and softening hard masses in the full side, and the Radix Astragali and Colla Corii Asini have benefiting vital QI and blood in the side, the effect of supplementing QI and nourishing YIN, perfect square is to liver cancer patient turbid damp pyretic toxicity phlegm and blood stasis, liver spleen two is hindered, deficiency of both QI and YIN, QI and blood is two thanks to have better curative effect.
The present invention also carries out active component research to above-described Chinese medicine compound, a kind of polyoses extract with antihepatocarcinoma effect is provided, and it prepares by the following method: get the Chinese medicine compound that the above parts by weight is formed, decoct with water, filter, get filtrate concentrating, concentrated solution adds the alcoholic solution mixing, places precipitation, centrifugal, get precipitation, get the polysaccharide crude extract, get the polysaccharide crude extract and add the water heating for dissolving, add the chloroform-n-butanol extraction of equivalent, violent jolting, behind the standing demix, isolate water layer, remove the intermediary jello of organic layer, so repeatedly till jello eliminates, collect aqueous solution at last, concentrate, drying gets polyoses extract.
Above-described preparation method with polyoses extract of antihepatocarcinoma effect, specifically may further comprise the steps: (1) gets the Chinese medicine compound that the above parts by weight is formed, decoct with water, filter, get filtrate concentrating, concentrated solution adds the alcoholic solution mixing, place precipitation, centrifugal, get precipitation, get the polysaccharide crude extract, standby; (2) get the polysaccharide crude extract that step (1) prepares, add the water heating for dissolving, chloroform-the n-butyl alcohol that adds equivalent extracts, and violent jolting is behind the standing demix, isolate water layer, remove the intermediary jello of organic layer, till jello eliminates, collect aqueous solution at last so repeatedly, concentrated, dry, get polyoses extract.
As preferred version, above-described preparation method with polyoses extract of antihepatocarcinoma effect, the middle amount of water of step (1) is 10～15 times of Chinese medicine compound medical material amount, decocting number of times is 1 to 3 time, decocts 0.5～1 hour at every turn.
As preferred version, above-described preparation method with polyoses extract of antihepatocarcinoma effect, add ethanol in the step (1), making in the concentrated solution concentration of ethanol reach 70～85% precipitates, the operation purpose of this method is that polysaccharide material is precipitated, thereby is separated with other alcohol dissolubility impurity.The present invention can effectively remove alcohol dissolubility impurity by water-soluble alcohol deposition method more than 2 to 3 times, obtains the higher polyoses extract of purity.
As preferred version, above-described preparation method with Chinese medicine compound of antihepatocarcinoma effect, adopting volume ratio in the step (2) is that chloroform-n-butyl alcohol of 5: 1 extracts, and can be effectively impurity such as the protein that mixes in the polysaccharide and pigment be removed.Can further improve the purity of polysaccharide, guarantee curative effect.
The application of Chinese medicine compound with antihepatocarcinoma effect provided by the invention in preparation treatment liver-cancer medicine can be made Chinese medicine compound extract and pharmaceutically acceptable carrier the medicine of capsule, microcapsule, tablet, granule or oral liquid formulation.
When making tablet, the Chinese medicine compound with antihepatocarcinoma effect provided by the invention, adds magnesium stearate lubricant when needing Chinese medicine compound and carrier lactose or corn starch with antihepatocarcinoma effect, mix homogeneously, and tabletting is made tablet then.Handle had Chinese medicine compound and the carrier lactose or the corn starch mix homogeneously of antihepatocarcinoma effect, granulate, the encapsulated then capsule of making when the Chinese medicine compound with antihepatocarcinoma effect provided by the invention was made capsule.When the Chinese medicine compound with antihepatocarcinoma effect provided by the invention is made granule, the Chinese medicine compound with antihepatocarcinoma effect and diluent lactose or corn starch mix homogeneously, granulate, drying is made granule.
The application of polyoses extract with antihepatocarcinoma effect provided by the invention in preparation treatment liver-cancer medicine can be made polyoses extract in the Chinese medicine compound and pharmaceutically acceptable carrier the medicine of capsule, microcapsule, tablet, granule or oral liquid formulation.
Beneficial effect: the Chinese medicine compound with antihepatocarcinoma effect provided by the invention is compared with prior art and is had the following advantages: (1) Chinese medicine compound with antihepatocarcinoma effect provided by the invention, choose the material combination prescription according to the pathogenesis of Chinese medical theory and hepatocarcinoma, the each component proportioning is scientific and reasonable, pharmacological evaluation and clinical experiment result show that Chinese medicine compound provided by the invention has the effect of good anti-hepatocarcinoma, can not only obviously improve patient's subjective symptoms, improve survival rate, improve biochemical indicator, dwindle the focus scope, and life-time service has no adverse reaction, and the Chinese medicine compound with antihepatocarcinoma effect provided by the invention can become the medicine of various dosage forms easily with pharmaceutically acceptable preparing carriers, convenient clinical taking.
(2) the present invention studies the Chinese medicine compound active component, from Chinese medicine compound, separate the polyoses extract that obtains having antihepatocarcinoma effect, the present invention also provides the preparation method of Chinese medicine compound polyoses extract, this method is workable, preparation-obtained purity of polysaccharide height, can reach 90%, and the preparation method cost is low, environmental protection, can realize industrialized great production.
The specific embodiment: according to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand that the described concrete material proportion of embodiment, process conditions and result thereof only are used to illustrate the present invention, and should also can not limit the present invention described in detail in claims.
Embodiment 1 has the Chinese medicine compound of antihepatocarcinoma effect, it is to be made by the raw material of following parts by weight: get Herba Euphorbiae Helioscopiae 12g, Radix Rhapontici 12g, Rhizoma Curcumae 12g, Radix Astragali 15g, Colla Corii Asini 12g, add 15 times of water gagings and decoct 2 times, each 1 hour, filter and collect extracting solution, be concentrated to 0.5g crude drug/ml, obtain the Chinese medicine compound water extract.Get Chinese medicine compound water extract 20g and add medical starch 16g, mix homogeneously adds water and makes soft material, crosses 20 mesh sieves and granulates, drying is made granule, incapsulates, every 0.2g, embodiment 2 has the Chinese medicine compound of antihepatocarcinoma effect, and it is to be made by the raw material of following parts by weight: get Herba Euphorbiae Helioscopiae 6g, Radix Rhapontici 6g, Rhizoma Curcumae 6g, Radix Astragali 8g, Colla Corii Asini 6g adds 10 times of water gagings and decocts each 0.5 hour 3 times, filter and collect extracting solution, be concentrated to 0.5g crude drug/ml, obtain the Chinese medicine compound water extract.Get Chinese medicine compound water extract 20g and add lactose 10g or corn starch 10g mix homogeneously, granulate, drying is made granule.
Embodiment 3 has the Chinese medicine compound of antihepatocarcinoma effect, it is to be made by the raw material of following parts by weight: get Herba Euphorbiae Helioscopiae 4g, Radix Rhapontici 4g, Rhizoma Curcumae 4g, Radix Astragali 5g, Colla Corii Asini 4g, add 12 times of water gagings and decoct 2 times, each 1 hour, filter and collect extracting solution, be concentrated to 0.5g crude drug/ml, obtain the Chinese medicine compound water extract.Get Chinese medicine compound water extract 20g and add corn starch 20g, add magnesium stearate lubricant 5g, mix homogeneously, tabletting is made tablet then.
Embodiment 4 has the Chinese medicine compound of antihepatocarcinoma effect, it is to be made by the raw material of following parts by weight: get Herba Euphorbiae Helioscopiae 10g, Radix Rhapontici 10g, Rhizoma Curcumae 10g, Radix Astragali 12g, Colla Corii Asini 10g, add 12 times of water gagings and decoct 2 times, each 1 hour, filter and collect extracting solution, be concentrated to 0.5g crude drug/ml, obtain the Chinese medicine compound water extract.Get the dissolving of Chinese medicine compound water extract adding distil water, add syrup and make oral liquid.
Embodiment 5 has the preparation (1) of the Chinese medicine compound polyoses extract of antihepatocarcinoma effect and gets the Chinese medicine compound water extract that embodiment 1 prepares, add ethanol, mixing, make concentration of ethanol reach 80%, place precipitation 12 hours, centrifugal, get precipitation, be dissolved in water then, repeat above water-soluble precipitate with ethanol 3 times again, obtain the polysaccharide crude extract, standby; (2) get the polysaccharide crude extract that step (1) prepares, add the water heating for dissolving, the volume ratio that adds equivalent is that chloroform-n-butyl alcohol of 5: 1 extracts, violent jolting 20 minutes, behind the standing demix, isolate water layer, remove the intermediary jello of organic layer, so repeatedly till jello eliminates, collect aqueous solution at last, upper water liquid is concentrated into 10g crude drug/ml, and 60 ℃ of vacuum dryings get the Chinese medicine compound polyoses extract.
Embodiment 6 has the preparation (1) of the Chinese medicine compound polyoses extract of antihepatocarcinoma effect and gets the Chinese medicine compound water extract that embodiment 2 prepares, add ethanol, mixing, make concentration of ethanol reach 85%, place precipitation 12 hours, centrifugal, get precipitation, be dissolved in water then, repeat above water-soluble precipitate with ethanol 4 times again, obtain the polysaccharide crude extract, standby; (2) get the polysaccharide crude extract that step (1) prepares, add the water heating for dissolving, the volume ratio that adds equivalent is that chloroform-n-butyl alcohol of 5: 1 extracts, violent jolting 20 minutes, behind the standing demix, isolate water layer, remove the intermediary jello of organic layer, so repeatedly till jello eliminates, collect aqueous solution at last, upper water liquid is concentrated into 10g crude drug/ml, and 60 ℃ of vacuum dryings get the Chinese medicine compound polyoses extract.
Embodiment 7 has the preparation (1) of the Chinese medicine compound polyoses extract of antihepatocarcinoma effect and gets the Chinese medicine compound water extract that embodiment 3 prepares, add ethanol, mixing, make concentration of ethanol reach 75%, place precipitation 12 hours, centrifugal, get precipitation, be dissolved in water then, repeat above water-soluble precipitate with ethanol 4 times again, obtain the polysaccharide crude extract, standby; (2) get the polysaccharide crude extract that step (1) prepares, add the water heating for dissolving, the volume ratio that adds equivalent is that chloroform-n-butyl alcohol of 5: 1 extracts, violent jolting 20 minutes, behind the standing demix, isolate water layer, remove the intermediary jello of organic layer, so repeatedly till jello eliminates, collect aqueous solution at last, upper water liquid is concentrated into 10g crude drug/ml, and 60 ℃ of vacuum dryings get the Chinese medicine compound polyoses extract.
Embodiment 8 anti-hepatocarcinoma experimentatioies are to the inhibited proliferation of people's hepatocarcinoma SMMZ-7721: SMMC-7721 is containing the RPMI-1640 culture fluid of 10% calf serum, and 37 ℃, 5%CO
2Cultivate under the condition, change culture fluid every other day.After treating cell monolayer confluent cultures bottle, use 0.25% trypsinization, behind 2～3min, treat that the cell circle contracts under the mirror, when more slit appears in iuntercellular, promptly add complete RPMI-1640 culture fluid and stop digestion, and blow and beat into the individual cells suspension gently with sharp suction pipe, mirror is counting down, cell about 10
7Individual/L, add culture fluid by 1: 2 and divide bottle, go down to posterity.
The trophophase SMMC-7721 cell of taking the logarithm, digestion, resuspended, regulating cell concentration is 5 * 10
4Individual/mL, be seeded to 4 96 parallel well culture plates, every hole 0.2mL continues to cultivate after 24 hours, and per 6 holes are one group, cell culture fluid is abandoned in suction, every group is added by cell culture fluid and is mixed with the Chinese medicine compound polyoses extract that the Chinese medicine compound extract that prepared by embodiment 1 and 2 and embodiment 5 and 6 prepare, every hole 0.2mL, other has 6 hole matched groups, and every hole adds the 0.2mL cell culture fluid.After cultivating 24 hours, culture fluid is abandoned in suction, new cell culture fluid 0.2mL is added in every hole, and add the MTT (preparing) of 20 μ L 5mg/mL with PBS, and continue to cultivate after 4 hours, inhale and abandon culture fluid, every hole adds 0.15mLDMSO, jolting 30min, the fully purple Jia Za crystallization that generates in the dissolved cell is measured every hole absorbance (OD value) down in 490nm.Concrete experimental result sees Table 1 and table 2.
Table 1 Chinese medicine compound extract to the SMMC-7721 cell inhibitory rate (X ± SD, n=6)
Annotate: * compares P＜0.01 and shows that by the experimental result of table 1 Chinese medicine compound provided by the invention can significantly suppress the propagation of people's hepatocarcinoma SMMZ-7721 cell, has good antihepatocarcinoma effect with the blank group, be expected to be prepared into new medicines resistant to liver cancer.
Table 2 Chinese medicine compound polyoses extract to people's hepatocarcinoma SMMZ-7721 cell inhibitory rate (X ± SD, n=6)
Annotate: * compares P＜0.01 and is shown by the experimental result of table 2 with the blank group, the polyoses extract that obtains from the Chinese medicine compound extraction provided by the invention has also shown the proliferation function of stronger inhibition people hepatocarcinoma SMMZ-7721 cell, also have antihepatocarcinoma effect preferably, be expected to be prepared into new medicines resistant to liver cancer.
The present invention simultaneously with liver cancer patient as object of study, carry out clinical observation on the therapeutic effect, through taking Chinese medicine compound provided by the invention in 3 months or extracting the polyoses extract that obtains from Chinese medicine compound, the subjective symptoms of liver cancer patient is significantly improved, various biochemical indicators be improved significantly, the focus scope is dwindled, and has shown the good anticancer effect of setting upright.
The present invention is according to the Chinese medical theory determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs, pathogeny according to hepatocarcinoma, carry out scientific composition, show through experimental result, Chinese medicine compound provided by the invention and the polyoses extract that obtains from the Chinese medicine compound extraction thereof, cell experiment and clinical and experimental study result proof has good antitumaous effect, and patient's body constitution be improved significantly, therefore Chinese medicine compound provided by the invention and extract the polyoses extract that obtains from Chinese medicine compound and be expected to develop and become the medicine that a new generation prevents and treats hepatocarcinoma.
Above embodiment only is explanation technical conceive of the present invention and characteristics; its purpose is to allow the people that is familiar with this technology understand content of the present invention and is implemented; can not limit protection scope of the present invention with this; all equivalences that spirit is done according to the present invention change or modify, and all should be encompassed in protection scope of the present invention.
Claims (10)
1. the Chinese medicine compound with antihepatocarcinoma effect is characterized in that, it is to be made by the raw material of following parts by weight:
4 ~ 12 parts of Herba Euphorbiae Helioscopiae, 4 ~ 12 parts of Radix Rhapontici, 4 ~ 12 parts of Rhizoma Curcumae, 5 ~ 15 parts of the Radixs Astragali, 4 ~ 12 parts in Colla Corii Asini.
2. the Chinese medicine compound with antihepatocarcinoma effect according to claim 1 is characterized in that, it is to be made by the raw material of following parts by weight:
6 ~ 10 parts of Herba Euphorbiae Helioscopiae, 6 ~ 10 parts of Radix Rhapontici, 6 ~ 10 parts of Rhizoma Curcumae, 8 ~ 12 parts of the Radixs Astragali, 6 ~ 10 parts in Colla Corii Asini.
3. polyoses extract with antihepatocarcinoma effect, it is characterized in that it prepares by the following method: the weighting profit requires 1 or 2 described parts by weight of traditional Chinese medicines compound recipes, decocts with water, filter, get filtrate concentrating, concentrated solution adds the alcoholic solution mixing, places precipitation, centrifugal, get precipitation, get the polysaccharide crude extract, get the polysaccharide crude extract and add the water heating for dissolving, add the chloroform-n-butanol extraction of equivalent, violent jolting, behind the standing demix, isolate water layer, remove the intermediary jello of organic layer, so repeatedly till jello eliminates, collect aqueous solution at last, concentrate, drying gets polyoses extract.
4. the described preparation method with polyoses extract of antihepatocarcinoma effect of claim 3 is characterized in that may further comprise the steps:
(1) the weighting profit requires 1 or 2 described parts by weight of traditional Chinese medicines compound recipes, decocts with water, and filters, and gets filtrate concentrating, and concentrated solution adds the alcoholic solution mixing, places precipitation, centrifugal, gets precipitation, gets the polysaccharide crude extract, and is standby;
(2) get the polysaccharide crude extract that step (1) prepares, add the water heating for dissolving, chloroform-the n-butyl alcohol that adds equivalent extracts, and violent jolting is behind the standing demix, isolate water layer, remove the intermediary jello of organic layer, till jello eliminates, collect aqueous solution at last so repeatedly, concentrated, dry, get polyoses extract.
5. the preparation method with polyoses extract of antihepatocarcinoma effect according to claim 4 is characterized in that, the middle amount of water of step (1) is 10 ~ 15 times of Chinese medicine compound medical material amount, and decocting number of times is 1 to 3 time, decocts 0.5 ~ 1 hour at every turn.
6. the preparation method with polyoses extract of antihepatocarcinoma effect according to claim 4 is characterized in that, adds ethanol in the step (1), makes in the concentrated solution concentration of ethanol reach 70 ~ 85% and precipitates.
7. the preparation method with Chinese medicine compound of antihepatocarcinoma effect according to claim 4 is characterized in that, the volume ratio of the chloroform-n-butyl alcohol described in the step (2) is 5:1.
8. claim 1 or the 2 described application of Chinese medicine compound in preparation treatment liver-cancer medicine with antihepatocarcinoma effect.
9. the application of the Chinese medicine compound with antihepatocarcinoma effect according to claim 8 in preparation treatment liver-cancer medicine is characterized in that Chinese medicine compound extract and pharmaceutically acceptable carrier are made the medicine of capsule, microcapsule, tablet, granule or oral liquid formulation.
10. the described application of polyoses extract in preparation treatment liver-cancer medicine of claim 3 with antihepatocarcinoma effect.
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| CN1313107C (en) * | 2005-06-29 | 2007-05-02 | 马宝瑜 | Medicine for treating tumor |
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Application publication date: 20110615 |