CN102087221A - Method for rapidly determining dissolved oxygen in water - Google Patents
Method for rapidly determining dissolved oxygen in water Download PDFInfo
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- CN102087221A CN102087221A CN 201010603771 CN201010603771A CN102087221A CN 102087221 A CN102087221 A CN 102087221A CN 201010603771 CN201010603771 CN 201010603771 CN 201010603771 A CN201010603771 A CN 201010603771A CN 102087221 A CN102087221 A CN 102087221A
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Abstract
The invention discloses a method for rapidly determining dissolved oxygen in water. The method is characterized by using a water sampler, a test tube or colorimetric tube, reagents, a dropper and a dissolved oxygen reference card, wherein the water sampler comprises a soft bottle with a rubber stopper; the rubber stopper is provided with an air tube and a water tube; the air tube and the water tube are respectively provided with a valve card; the reagents include anhydrous manganese sulfate and alkaline potassium iodide; and the dissolved oxygen reference card is a standard color gradation card made according to the colors of the precipitates produced by reaction between the standard water sample containing different dissolved oxygen and the reagents. The method comprises the following steps: 1. taking a water sample; 2. fixing the water sample; and 3. determining the dissolved oxygen value of the water sample through colorimetry. The method has the following characteristics: the oxygen in the air can be avoided from being dissolved in the water sample by using the water sampler for acquiring water samples; and the color of the flocculent precipitate generated by the water sample is directly compared with the dissolved oxygen reference card and the method is simple, fast and applicable, the dissolved oxygen is measured immediately by using the method and the result is obtained within 3-5 minutes, so the method has site guidance significance.
Description
Technical field
The invention belongs to the dissolved oxygen assay method, be specifically related to a kind of rapid assay methods of oxygen in water.
Background technology
Dissolved oxygen is the fish bases for the survival, with fish growth, the breeding closely related, dissolved oxygen in water abundance, fish body normal growth, the dissolved oxygen in water deficiency, even if the fish food abundance, be in optimum growth temperature, fish do not grow yet, and disease-resistant anti-adversity is relatively poor, if do not take the bleeding technique measure, fish just raise the nose above water to breathe, even the death of the general pool, give to culture to produce and cause serious economy loss.
It is pond dissolved oxygen management that dissolved oxygen is measured, and promotes the key link of culture benefit.At present, dissolved oxygen is measured and is only rested on the laboratory, can't be in the production scene wide popularization and application, and reason has two technological difficulties: the one, be not fit to on-the-spot special hydrophore, in the sampling operation process, be difficult to avoid that oxygen dissolves in the water in the air, influence the result and judge; The 2nd, can not in time measure, the existing survey now used.And experimental determination standard solution every day all must be demarcated, and will have that the professional person demarcates, titration, and the producer is difficult to grasp, in addition the experimental determination time oversize, be difficult to having produced directive function.Finding in the production has relevant method for quick, but does not comprise special method or the step that oxygen dissolves in the collection water sample in the water in the air of avoiding, and the oxygen dissolving value of surveying not is the water sample actual value, is difficult to having produced directive function.
Summary of the invention
The rapid assay methods that the purpose of this invention is to provide a kind of oxygen in water of suitable production scene operation.
The technical solution adopted for the present invention to solve the technical problems is:
The rapid assay methods of oxygen in water comprises:
Use hydrophore, test tube or color comparison tube, reagent, dropper and dissolved oxygen reference card; Wherein, described hydrophore comprises the soft bottle of being with rubber stopper, and rubber stopper is provided with tracheae and water pipe, is respectively equipped with the valve card on tracheae and the water pipe; Described reagent is anhydrous manganous sulfate and alkaline potassium iodide; The standard color scale card of described dissolved oxygen reference card for making according to the precipitation color of standard water sample that contains different dissolved oxygen amounts and reagent reacting production;
Step is:
One, water sampling: flatten bottle, close the valve card on tracheae and the water pipe, water pipe is placed the sampling water layer, the valve card of opening on the water pipe is drawn water sample, after suction is finished, closes the valve card on the water pipe, water pipe is put in the bottom of test tube or color comparison tube, open valve card on the water pipe and the valve card on the tracheae, after allowing water sample flow into test tube or color comparison tube automatically and overflowing 2-3 times of volume, will continue the water pipe of flowing water and extract out;
Two, water sample stabilization: dropper inserted add reagent anhydrous slufuric acid manganese solution 2-3 in the color comparison tube of full water sample or the test tube and drip, reagent alkalescence liquor kalii iodide 4-6 drips, cover stopper, avoid air to enter, turn upside down and carry out precipitation reaction several times, placed 3-5 minute, and made to precipitate in the water all to be sunken to the pipe end;
Three, colorimetric is determined the water sample oxygen dissolving value: the precipitation color and the dissolved oxygen reference card of color comparison tube or test tube bottom are contrasted, determine the water sample oxygen dissolving value.
Principle:
Manganese sulfate generates Mn (OH) in alkaline medium
2White precipitate behind the dissolved oxygen DO, is produced H immediately in running into water body
2MnO
3Brown precipitate is along with H
2MnO
3The increase of amount, the water body color is progressively deepened.
The rapid assay methods of oxygen in water of the present invention has following characteristics:
The one, can utilize plastics system transfusion bottle or soft bottle self-control hydrophore to gather water sample and avoid that oxygen dissolves in the water sample in the air, low-carbon (LC), economy, environmental protection, be suitable for;
The 2nd, get the fixing step that dissolved oxygen is measured in the laboratory, the flocculent deposit color that produces with water sample directly and the dissolved oxygen reference card contrast, can determine the water sample oxygen dissolving value, standard solution demarcation, dissolved oxygen titration link have been saved, simple and direct, quick, suitable, now survey existing using, went out the result in 3-5 minute, have the on-the-spot guidance meaning, be in critical value, water hypoxia for the milky decidable for the pond dissolved oxygen as flocculent deposit, judge what of water body dissolved oxygen according to the brown depth, brown dark, then dissolved oxygen is abundant, and vice versa.
This method is suitable for the dissolved oxygen DO that detects the fish culture water body, and the scope of detecting is 2mg/L-8 mg/L, can judge breeding water body dissolved oxygen critical value 2mg/L fast.
Further specify technical scheme of the present invention below in conjunction with accompanying drawing.
Description of drawings
Fig. 1 is the synoptic diagram of the inventive method with the hydrophore water sampling.
Fig. 2 is the synoptic diagram of the inventive method with water sample injecting tube or color comparison tube.
Fig. 3 is the synoptic diagram that the inventive method is carried out colorimetric.
Embodiment
The rapid assay methods of oxygen in water comprises:
Use hydrophore, test tube or color comparison tube, reagent, dropper and dissolved oxygen reference card; Wherein, described hydrophore comprises the soft bottle 1 of being with rubber stopper 2 as shown in Figure 1, and rubber stopper 2 is provided with tracheae 6 and water pipe 3, is respectively equipped with valve card 5,4 on tracheae 6 and the water pipe 3; It is the anhydrous manganous sulfate of 300 g/L-400 g/L and the alkaline potassium iodide that concentration is 550g/L-650 g/L that described reagent adopts concentration; The standard color scale card of described dissolved oxygen reference card 9 for making according to the precipitation color of standard water sample that contains different dissolved oxygen amounts and reagent reacting production seen Fig. 3;
Step is:
One, water sampling: as shown in Figure 1, flatten bottle 1, close the valve card 5,4 on tracheae 6 and the water pipe 3, water pipe 3 is placed sampling water layer 7, the valve card of opening on the water pipe 34 is drawn water sample, after suction is finished, close the valve card 4 on the water pipe 3, water pipe 3 is put in the bottom (referring to Fig. 2) of test tube or the color comparison tube 8 of 10-25mL, open valve card 4 on the water pipe 3 and the valve card 5 on the tracheae 6, after allowing water sample flow into automatically and overflowing 2-3 times of test tube or color comparison tube volume, will continue the water pipe 4 of flowing water and extract out;
Two, water sample stabilization: dropper inserted add reagent anhydrous manganous sulfate 2-3 in the color comparison tube of full water sample or the test tube 8 and drip, reagent alkalescence potassium iodide 4-6 drips, and covers stopper, avoid air to enter, turn upside down and carry out precipitation reaction several times, placed 3-5 minute, make to precipitate in the water all to be sunken to the pipe end;
Three, colorimetric is determined the water sample oxygen dissolving value: as shown in Figure 3, the precipitation color and the dissolved oxygen reference card 9 of color comparison tube or test tube 8 bottoms contrasted, determine the water sample oxygen dissolving value.
The color range of dissolved oxygen reference card 9 is divided into four kinds, milky a ', light brown b ', in brown c ' and dark-brown d ' represent oxygen dissolving value in the water sample for less than 2mg/L, 4-5mg/L, 6-7mg/L, greater than 8mg/L respectively.If the flocculent deposit color in the water sample of four color comparison tubes or test tube 8 be respectively milky a, light brown b, in brown c and dark-brown d, the oxygen dissolving value that both can be drawn them more at once is respectively less than 2mg/L, 4-5mg/L, 6-7mg/L, greater than 8mg/L.
Claims (1)
1. the rapid assay methods of an oxygen in water is characterized in that comprising:
Use hydrophore, test tube or color comparison tube, reagent, dropper and dissolved oxygen reference card; Wherein, described hydrophore comprises the soft bottle of being with rubber stopper, and rubber stopper is provided with tracheae and water pipe, is respectively equipped with the valve card on tracheae and the water pipe; Described reagent is anhydrous manganous sulfate and alkaline potassium iodide; The standard color scale card of described dissolved oxygen reference card for making according to the precipitation color of standard water sample that contains different dissolved oxygen amounts and reagent reacting production;
Step is:
One, water sampling: flatten bottle, close the valve card on tracheae and the water pipe, water pipe is placed the sampling water layer, the valve card of opening on the water pipe is drawn water sample, after suction is finished, closes the valve card on the water pipe, water pipe is put in the bottom of test tube or color comparison tube, open valve card on the water pipe and the valve card on the tracheae, after allowing water sample flow into test tube or color comparison tube automatically and overflowing 2-3 times of volume, will continue the water pipe of flowing water and extract out;
Two, water sample stabilization: dropper inserted add reagent anhydrous slufuric acid manganese solution 2-3 in the color comparison tube of full water sample or the test tube and drip, reagent alkalescence liquor kalii iodide 4-6 drips, and covers stopper, avoid air to enter, turn upside down and carry out precipitation reaction several times, placed 3-5 minute, make precipitation all be sunken to the pipe end;
Three, colorimetric is determined the water sample oxygen dissolving value: the precipitation color and the dissolved oxygen reference card of color comparison tube or test tube bottom are contrasted, determine the water sample oxygen dissolving value.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010106037711A CN102087221B (en) | 2010-12-24 | 2010-12-24 | Method for rapidly determining dissolved oxygen in water |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010106037711A CN102087221B (en) | 2010-12-24 | 2010-12-24 | Method for rapidly determining dissolved oxygen in water |
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| CN102087221A true CN102087221A (en) | 2011-06-08 |
| CN102087221B CN102087221B (en) | 2012-05-23 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103278494A (en) * | 2013-04-24 | 2013-09-04 | 中国科学院力学研究所 | Method and experimental device for detecting dissolved oxygen by optical and electrical combination |
| CN105572094A (en) * | 2016-02-03 | 2016-05-11 | 环境保护部南京环境科学研究所 | Detection device and detection method capable of measuring dissolved oxygen at different horizontal positions and different heights of mud-water interface simultaneously in situ |
| CN105606578A (en) * | 2016-02-03 | 2016-05-25 | 环境保护部南京环境科学研究所 | Detection device and detection method for simultaneously measuring dissolved oxygen at different heights on muddy water interface in situ |
| CN106568770A (en) * | 2016-11-11 | 2017-04-19 | 上海利康消毒高科技有限公司 | Preparation method of rapid test card for rapidly testing peroxyacetic acid concentration |
| CN107037015A (en) * | 2016-02-03 | 2017-08-11 | 环境保护部南京环境科学研究所 | While the detection means and its detection method of in site measurement submerged plant blade-water termination different height dissolved oxygen |
| CN108489972A (en) * | 2018-03-02 | 2018-09-04 | 成都虹波钼业有限责任公司 | A kind of method of low content molybdenum in quick measurement solution |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110031589A (en) * | 2019-04-26 | 2019-07-19 | 山东省农业科学院农产品研究所 | The measuring method of dissolved oxygen in a kind of hydrophobic liquid |
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| JPH06180273A (en) * | 1992-12-15 | 1994-06-28 | Sharp Corp | Method and device for collecting sample |
| JPH1164225A (en) * | 1997-08-27 | 1999-03-05 | Miura Co Ltd | Method for removing air bubble in colorimetric measuring apparatus |
| CN2556637Y (en) * | 2002-08-11 | 2003-06-18 | 青岛海洋大学 | Colorimetric detector for dissolved oxygen content in pool |
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2010
- 2010-12-24 CN CN2010106037711A patent/CN102087221B/en not_active Expired - Fee Related
Patent Citations (3)
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|---|---|---|---|---|
| JPH06180273A (en) * | 1992-12-15 | 1994-06-28 | Sharp Corp | Method and device for collecting sample |
| JPH1164225A (en) * | 1997-08-27 | 1999-03-05 | Miura Co Ltd | Method for removing air bubble in colorimetric measuring apparatus |
| CN2556637Y (en) * | 2002-08-11 | 2003-06-18 | 青岛海洋大学 | Colorimetric detector for dissolved oxygen content in pool |
Non-Patent Citations (1)
| Title |
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| 《国外医学.卫生学分册》 19801231 李澄平 水中溶解氧的比色测定法 243-244 1 , 第04期 2 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103278494A (en) * | 2013-04-24 | 2013-09-04 | 中国科学院力学研究所 | Method and experimental device for detecting dissolved oxygen by optical and electrical combination |
| CN103278494B (en) * | 2013-04-24 | 2015-11-04 | 中国科学院力学研究所 | A kind of method of photoelectricity compound detection dissolved oxygen and experimental provision |
| CN105572094A (en) * | 2016-02-03 | 2016-05-11 | 环境保护部南京环境科学研究所 | Detection device and detection method capable of measuring dissolved oxygen at different horizontal positions and different heights of mud-water interface simultaneously in situ |
| CN105606578A (en) * | 2016-02-03 | 2016-05-25 | 环境保护部南京环境科学研究所 | Detection device and detection method for simultaneously measuring dissolved oxygen at different heights on muddy water interface in situ |
| CN107037015A (en) * | 2016-02-03 | 2017-08-11 | 环境保护部南京环境科学研究所 | While the detection means and its detection method of in site measurement submerged plant blade-water termination different height dissolved oxygen |
| CN105572094B (en) * | 2016-02-03 | 2019-07-26 | 环境保护部南京环境科学研究所 | The detection device and its detection method of energy while in situ measurement water sludge interface different level position different height dissolved oxygen |
| CN105606578B (en) * | 2016-02-03 | 2019-07-26 | 环境保护部南京环境科学研究所 | The detection device and its detection method of in situ measurement water sludge interface different height dissolved oxygen simultaneously |
| CN107037015B (en) * | 2016-02-03 | 2019-07-30 | 环境保护部南京环境科学研究所 | In situ measurement submerged plant blade-water termination different height dissolved oxygen detection device and its detection method simultaneously |
| CN106568770A (en) * | 2016-11-11 | 2017-04-19 | 上海利康消毒高科技有限公司 | Preparation method of rapid test card for rapidly testing peroxyacetic acid concentration |
| CN108489972A (en) * | 2018-03-02 | 2018-09-04 | 成都虹波钼业有限责任公司 | A kind of method of low content molybdenum in quick measurement solution |
| CN108489972B (en) * | 2018-03-02 | 2021-03-02 | 成都虹波钼业有限责任公司 | Method for rapidly determining low-content molybdenum in solution |
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| CN102087221B (en) | 2012-05-23 |
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