CN102071136A - Array micro polymerase chain reaction chip - Google Patents
Array micro polymerase chain reaction chip Download PDFInfo
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- CN102071136A CN102071136A CN2009102415361A CN200910241536A CN102071136A CN 102071136 A CN102071136 A CN 102071136A CN 2009102415361 A CN2009102415361 A CN 2009102415361A CN 200910241536 A CN200910241536 A CN 200910241536A CN 102071136 A CN102071136 A CN 102071136A
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Abstract
The invention discloses an array micro polymerase chain reaction chip, and relates to desoxyribonucleic acid (DNA) amplification technology. The chip comprises a temperature control system, a heating chip and a reaction cavity; the heating chip and the reaction cavity are arranged separately; and during use, after a reaction cavity framework is sleeved on the external circle of an insulating substrate, the reaction cavity framework is pressed down so that the lower surface of the bottom of a reaction tank is tightly jointed with the upper surface of a heating electrode, sample solution is injected into the reaction tank, and then the chip is started. The amplification reaction can be performed by sticking a clean disposable polydimethylsiloxane (PDMS) micro array to the heating chip every time. The reaction chip can microminiaturize polymerase chain reaction (PCR), fulfills the purposes of microminiaturization, lightness, quickness and saving, and can also solve the problems of biocompatibility difficult to be overcome by microminiaturization and pollution of the PCR.
Description
Technical field
The present invention relates to thymus nucleic acid (DNA) amplification technique field, to the effect that utilize polymerase chain reaction technology to realize the segmental external rapid amplifying of specific thymus nucleic acid (DNA).
Background technology
Polymerase chain reaction technology can be realized the segmental external rapid amplifying of special thymus nucleic acid (DNA), and the application in various fields such as life science, biomedicine, legal medical expert's evaluation is very extensive.
Through the development of decades, polymerase chain reaction (PCR) technology reaches its maturity.Desk-top polymerase chain reaction (PCR) the amplification instrument of selling on the market now also is of a great variety, and function is different.But shortcomings such as they generally all have, and volume is big, quality heavy, slow, the required amount of reagent of temperature rate is many.Utilizing microelectromechanical systems (MEMS) technology that conventional polymeric polymerase chain reaction (PCR) amplification experiment is moved on to small biochip from desk-top amplification instrument realizes, promptly be made into the laboratory (Lab-on-Chip) on the chip, can well remedy the defective of desk-top instrument, have little, light, fast, economize or the like plurality of advantages.
But undeniablely be in the process of using and promoting, also to be faced with many difficulties and challenge based on polymerase chain reaction (PCR) chip of microelectromechanical systems (MEMS) technology.For example, the bio-compatibility problem of microelectromechanical systems (MEMS) process materials (as silicon, glass etc.) and polymerase chain reaction (PCR) reagent, the pollution problem of polymerase chain reaction (PCR) etc.The existence of these problems has seriously restricted the development and the application of microminiaturized polymerase chain reaction (PCR),
In sum, develop a kind of novel polymerase chain reaction (PCR) amplification method, make it can overcome the shortcoming of conventional desktop amplification instrument, the new problem that can avoid microelectromechanical systems (MEMS) technology to bring again has important significance for theories and practical value.
Summary of the invention
The objective of the invention is to disclose a kind of array polymerase chain reaction (PCR) chip that declines, with the shortcoming that overcomes conventional desktop amplification instrument and solve the problem that microminiaturized polymerase chain reaction (PCR) faces, this chip performance is stable, cheap, volume is little, little heating chip and little reaction chamber array are separated, do the amplified reaction simple and fast.
For achieving the above object, technical solution of the present invention is:
A kind of array PCR chip that declines comprises temperature control system, heating chip and reaction chamber; Its heating chip is to separate setting with reaction chamber;
Heating chip is electrically connected with temperature control system, and heating chip comprises insulating substrate, heating electrode and temperature sensor, wherein, in the sputter of insulating substrate upper surface heating electrode and temperature sensor is arranged; Heating electrode is annular disk-shaped, the intermediate radial of annular disc, at the insulating substrate upper surface raceway groove is arranged, and temperature sensor probe is embedded in the raceway groove; The insulating substrate lower surface is provided with groove with respect to heating electrode and temperature sensor, and making insulating substrate side section is inverted concave;
Reaction chamber comprises framework and reaction tank, and framework comprises side, end face, and side is fixed in the end face periphery, quadrature is downward, and the shape that side surrounds and the shape of insulating substrate are suitable; The reaction tank mouth is located at end face, and the pond body extends downwards, and reaction tank is circle, side or Polygons cylinder, and the reaction tank bottom surface is the plane, and reaction tank base area and heating electrode area are suitable;
The reaction tank number equates in heating chip and the reaction chamber, is at least four, becomes the array setting;
During use, press after reaction chamber aimed at heating electrode, make the closely company of subsides of reaction chamber lower surface and heating electrode upper surface, in reaction tank, inject sample liquid, start then.
The described array PCR chip that declines, its described insulating substrate is a silicon chip; Heating electrode and temperature sensor are that platinum is made, and the resistance value of the resistance value of the shape of each heating chip, size, heating electrode and temperature sensor is all identical in the array; Reaction chamber is the disposable device that polydimethylsiloxane (PDMS) material is made, and the shape of each reaction tank and volume are identical in the array.
The described array PCR chip that declines, its described temperature control system comprises multiplex temperature collection circuit, signal amplification circuit, A/D change-over circuit, microcontroller proportion integration differentiation (PID) setter, performer; Wherein, the multiplex temperature collection circuit is electrically connected with microcontroller proportion integration differentiation (PID) setter input terminus through signal amplification circuit, A/D change-over circuit, and microcontroller proportion integration differentiation (PID) conditioner outlet end is electrically connected with performer;
Microcontroller proportion integration differentiation (PID) setter is provided with touch-screen;
A plurality of input terminuss of multiplex temperature collection circuit are electrically connected with temperature sensor, and a plurality of output terminals of performer are electrically connected with heating electrode.
The described array PCR chip that declines, its described microcontroller proportion integration differentiation (PID) setter or external computer, and with the computer bidirectional communication; Microcontroller proportion integration differentiation (PID) conditioner outlet end sends width modulation (PWM) signal, with the control performer sequential control is carried out in the temperature intensification or the cooling of each road heating chip in the array.
The present invention fully combines the advantage of microelectromechanical systems (MEMS) technology and the good characteristic of polydimethylsiloxane (PDMS) material, has both realized the microminiaturization of polymerase chain reaction (PCR), has plurality of advantages such as little, light, fast, province; Bio-compatibility and polymerase chain reaction (PCR) pollution problems have been avoided simultaneously again.Because the hommization of intellectuality, automatization and the operation interface of temperature control system, miniature polymerase chain reaction (PCR's) is flexible and convenient to use, easily left-hand seat.
Description of drawings
Fig. 1 is the integrated morphology synoptic diagram of single heating electrode and temperature sensor among the present invention;
Fig. 2 is the manufacture craft schematic flow sheet of little heating chip among the present invention, wherein:
Fig. 2 (a) all growthing silica and silicon nitride on the two sides of silicon chip;
Fig. 2 (b) is at silicon chip front whirl coating and make pattern by lithography;
Fig. 2 (c) is at the positive sputter platinum of silicon chip;
Fig. 2 (d) digs pit in the silicon chip back corrosion;
Fig. 3 is the array structure synoptic diagram of little heating chip among the present invention;
Fig. 4 is a mould structure synoptic diagram of making the little reaction chamber array of polydimethylsiloxane (PDMS) among the present invention;
Fig. 5 is the structural representation of the little reaction chamber array of polydimethylsiloxane among the present invention (PDMS);
Fig. 6 is the overall structure functional diagram of temperature control system among the present invention.
Embodiment
A kind of array polymerase chain reaction (PCR) chip that declines, utilize microelectromechanical systems (MEMS) technology and material (as silicon, platinum etc.) to make little heating chip, utilize stable performance, cheap polydimethylsiloxane (PDMS) material to make little reaction chamber array, and little heating chip and little reaction chamber array are separated.When doing amplified reaction at every turn, get a clean little reaction chamber array and be bonded on little heating chip, control little heating chip by temperature control system and carry out temperature cycle, just can realize amplified reaction.
A kind of array of the present invention polymerase chain reaction (PCR) chip that declines mainly comprises following three parts, and the 26S Proteasome Structure and Function of every part is as follows:
(1) little heating chip.Steps such as little heating chip utilizes microelectromechanical systems (MEMS) technology and material to make, and main processes comprises growthing silica and silicon nitride film, whirl coating photoetching, sputter, remove photoresist.For the purpose of a plurality of samples of realizing once increasing, the little heating electrode on the chip is made array.For the temperature distribution that makes each heating electrode is more even, heater design is circularized discoid.Reserve a raceway groove in the middle of the disk, temperature sensor is embedded among the heating electrode, so just can monitor and control the temperature of heating electrode in real time accurately by this raceway groove.
(2) little reaction chamber array.In order to solve bio-compatibility difference and polymerase chain reaction (PCR) pollution problems that microminiaturized polymerase chain reaction (PCR) exists, the present invention adopts polydimethylsiloxane (PDMS) material of excellent property to make little reaction chamber.Polydimethylsiloxane (PDMS) is a kind of polymer organic polymer, and it has good bio-compatibility, resistant of high or low temperature and erosion resistance, not can with polymerase chain reaction (PCR) reagent generation chemical reaction; Its is cheap simultaneously, and it is very low to make disposable little reaction chamber array cost with it, and manufacture craft is simple.Therefore, polydimethylsiloxane (PDMS) is a kind of material that is fit to very much do little reaction chamber array.
(3) temperature control system.For the purpose of a plurality of samples of realizing once increasing, temperature control system is designed to can multi-channel sampling and the structure of multi-channel A/D conversion.The faint temperature signal that is collected by bridge diagram send instrument amplifier to amplify processing, and then send A/D converter to carry out digital-to-analog conversion, result according to conversion obtains current temperature value, and then carry out proportion integration differentiation (PID) by more current set(ting)value and regulate, control the working order of heating electrode at last by output pwm signal, thereby realize regulating in real time the purpose of heater temperature.
A kind of array of the present invention polymerase chain reaction (PCR) chip technology characteristics that decline are:
(1) separation property of little heating chip and reaction chamber array.In order to realize the microminiaturization of polymerase chain reaction (PCR), can solve some problems that microminiaturization brings again, the present invention is with little heating chip and little reaction chamber array separate design.Utilize the little heating chip of microelectromechanical systems (MEMS) fabrication techniques to realize the microminiaturization of polymerase chain reaction (PCR), utilize polydimethylsiloxane (PDMS) material to make disposable little reaction chamber array and solved bio-compatibility and polymerase chain reaction (PCR) pollution problems.
(2) array.In order to promote the efficient of amplification, the present invention is designed to array with heating electrode and little reaction chamber, and a plurality of samples so once just can increase.This occasion that need carry out a large amount of thymus nucleic acids (DNA) amplification in gene sequencing etc. has special advantages.
(3) integration of heating electrode and temperature sensor.Little heating chip mainly comprises two parts: heating electrode and temperature sensor, heating electrode are used for heating to reagent, and temperature sensor is used for controlling in real time Current Temperatures.The present invention is integrated together the two when making little heating chip, and temperature sensor just can reflect but preceding actual temperature is improved expanding effect more quickly and accurately like this.
(4) disposable little reaction chamber array.The main problem in microminiaturized polymerase chain reaction (PCR) is exactly contaminated easily.If at every turn before doing amplification experiment, all to take much time and energy goes to the cleaning reaction chamber, this can reduce the efficient of amplification undoubtedly to a great extent.And along with the development of microminiaturization, the cleaning meeting becomes more and more difficult, in case clean untotally, will cause the consequence that is difficult to estimate.The present invention utilizes cheap polydimethylsiloxane (PDMS) material to make disposable little reaction chamber array, gets a clean reaction chamber array at every turn when reacting and is bonded on the heating chip and just can carries out amplified reaction.So both save loaded down with trivial details cleaning, also stopped the generation that pollute polymerase chain reaction (PCR) fully.And because the cheapness of polydimethylsiloxane (PDMS), even if disposable use can not increase too many cost yet.
(5) multichannel of temperature control system.In order to cooperate little heating chip and little reaction chamber array, but temperature control system has been designed to the Controlling System of multi-channel sampling, multipath conversion.By cycle control, carry out when realizing a plurality of reaction each road.
(6) automatization of temperature control system and intellectuality.Temperature control adopts classical proportion integration differentiation (PID) to regulate algorithm, can regulate the dutycycle of pulse-width signal according to the difference of currency and set(ting)value, thus the working order of control heating electrode.As long as before amplification, set the temperature value of proportion integration differentiation (PID) adjusting parameter and each time period, do not need manual operation in the amplification procedure fully, just can finish amplified reaction repeatedly automatically.
Further describe concrete enforcement of the present invention below in conjunction with accompanying drawing.
As shown in Figure 1, each heating electrode and temperature sensor are integrated make a heating unit.Wherein the disk that lines are thicker is a heating electrode, and middle thinner lines are temperature sensors.Because the two closely integrates, so temperature sensor can detect the current temperature of well heater in real time accurately, delivers to temperature control system and carries out temperature control.
Fig. 2 is a general schema making little heating chip.(a) thermooxidizing one deck silicon oxide film on the silicon chip that cleans up, low-pressure chemical vapor phase deposition (LPCVD) one deck silicon nitride film again; (b), obtain the pattern of heating electrode and temperature sensor in silicon chip front whirl coating, photoetching, development; (c) utilize Lift_Off technology sputter one deck platinum, after removing photoresist with the acetone immersion, just can obtain heating electrode and temperature sensor; (d) in order to improve the speed of heating and cooling, the silicon chip back side is eroded a part.Fig. 3 is the array junctions composition of entire chip after completing, as seen from the figure, each above the chip a plurality of heating units line up array so that increase a plurality of samples simultaneously.
Fig. 4 is a mould structure synoptic diagram of making the little reaction chamber array of polydimethylsiloxane (PDMS).When making the little reaction chamber array of polydimethylsiloxane (PDMS), put into vacuum chamber after earlier polydimethylsiloxane (PDMS) liquid and solidifying agent being mixed fully by 10: 1 ratio and bleed with the bubble in the eliminating mixed solution; After then mixed solution being poured in the mould shown in Figure 4, put into the about little reaction chamber array of polydimethylsiloxane (PDMS) that just can obtain moulding in 1 hour of baking oven baking, as shown in Figure 5.As shown in Figure 5, on each little reaction chamber array a plurality of reaction tanks are arranged, and structure, the quantity of heating unit is corresponding on the structure of reaction tank, quantity and the little heating chip array.
Fig. 6 is the overall structure skeleton diagram of temperature control system.As we know from the figure, temperature control system can be divided into 4 parts substantially: temperature signal importation, processes temperature signal part, control signal output and operation interface part.Wherein, the input of temperature signal comprises that again signals collecting, signal amplify and three parts of A/D conversion of signal.In order to collect faint temperature signal, the signals collecting partial design is become the Kelvin double bridge structure, in order to suppress common mode as much as possible, to amplify temperature signal, signal amplifying part branch employing instrument amplifier.The main effect of signal processing is according to the magnitude relationship between current set temperature value and the actual temperature value, proportion of utilization integral differential (PID) is regulated algorithm, the pulse-width signal of an EDM Generator of Adjustable Duty Ratio of output, the switch working state of control heating electrode, and then the temperature of adjusting heating electrode makes it be tending towards set(ting)value.Operation interface also is the operator-machine-interface part, and friendly operator-machine-interface is the important component part of a good electronic product, and operator-machine-interface of the present invention mainly comprises touch-screen and PC two portions, in order to realize purposes such as parameter setting, process control, monitoring in real time.
Claims (4)
1. array PCR chip that declines comprises temperature control system, heating chip and reaction chamber; It is characterized in that: heating chip is to separate setting with reaction chamber;
Heating chip is electrically connected with temperature control system, and heating chip comprises insulating substrate, heating electrode and temperature sensor, wherein, in the sputter of insulating substrate upper surface heating electrode and temperature sensor is arranged; Heating electrode is annular disk-shaped, the intermediate radial of annular disc, at the insulating substrate upper surface raceway groove is arranged, and temperature sensor probe is embedded in the raceway groove; The insulating substrate lower surface is provided with groove with respect to heating electrode and temperature sensor, and making insulating substrate side section is inverted concave;
Reaction chamber comprises framework and reaction tank, and framework comprises side, end face, and side is fixed in the end face periphery, quadrature is downward, and the shape that side surrounds and the shape of insulating substrate are suitable; The reaction tank mouth is located at end face, and the pond body extends downwards, and reaction tank is a right cylinder, and the reaction tank bottom surface is the plane, and reaction tank base area and heating electrode area are suitable;
The reaction tank number equates in heating chip and the reaction chamber, is at least four, becomes the array setting;
During use, press after reaction chamber aimed at heating electrode, make the closely company of subsides of reaction chamber lower surface and heating electrode upper surface, in reaction tank, inject sample liquid, start then.
2. the array as claimed in claim 1 PCR chip that declines, it is characterized in that: described insulating substrate is a silicon chip; Heating electrode and temperature sensor are that platinum is made, and the resistance value of the resistance value of the shape of each heating chip, size, heating electrode and temperature sensor is all identical in the array; The disposable device that reaction chamber is made for the polydimethylsiloxane material, the shape of each reaction tank and volume are identical in the array.
3. the array as claimed in claim 1 PCR chip that declines, it is characterized in that: described temperature control system comprises multiplex temperature collection circuit, signal amplification circuit, A/D change-over circuit, microcontroller proportion integration differentiation (PID) setter, performer; Wherein, the multiplex temperature collection circuit is electrically connected with microcontroller proportional integral derivative controller input terminus through signal amplification circuit, A/D change-over circuit, and microcontroller proportional integral derivative controller output terminal is electrically connected with performer;
The microcontroller proportional integral derivative controller is provided with touch-screen;
A plurality of input terminuss of multiplex temperature collection circuit are electrically connected with temperature sensor, and a plurality of output terminals of performer are electrically connected with heating electrode.
4. the array as claimed in claim 3 PCR chip that declines is characterized in that: described microcontroller proportional integral derivative controller or external computer, and with the computer bidirectional communication; Microcontroller proportional integral derivative controller output terminal sends the PWM modulation signal, with the control performer sequential control is carried out in the temperature intensification or the cooling of each road heating chip in the array.
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| CN2009102415361A CN102071136A (en) | 2009-11-25 | 2009-11-25 | Array micro polymerase chain reaction chip |
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| CN2009102415361A CN102071136A (en) | 2009-11-25 | 2009-11-25 | Array micro polymerase chain reaction chip |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559482A (en) * | 2011-12-31 | 2012-07-11 | 中山大学达安基因股份有限公司 | Gene detection device |
| CN106337021A (en) * | 2016-08-23 | 2017-01-18 | 上海交通大学 | Large-area accurate temperature control system for polymerase chain reaction |
| CN106872542A (en) * | 2015-12-13 | 2017-06-20 | 安徽中医药高等专科学校 | The preparation of detachable single heat or cold, hot two-purpose electrode |
| CN106929408A (en) * | 2017-01-26 | 2017-07-07 | 深圳市尚维高科有限公司 | Portable micro-fluidic PCR instrument and gene samples fluorescence quantitative detecting method |
| CN107129933A (en) * | 2017-05-08 | 2017-09-05 | 大连大学 | One kind is based on electrically driven (operated) digital microcurrent-controlled pcr chip device |
| CN107656562A (en) * | 2016-07-26 | 2018-02-02 | 中国航空工业集团公司西安飞行自动控制研究所 | A kind of temperature control system for MEMS |
| CN109870205A (en) * | 2017-12-04 | 2019-06-11 | 上海新微技术研发中心有限公司 | Microfluidic flow meter and manufacturing method thereof |
| CN112020551A (en) * | 2019-03-29 | 2020-12-01 | 京东方科技集团股份有限公司 | Detection chip, use method thereof and reaction system |
| CN113106011A (en) * | 2021-03-29 | 2021-07-13 | 江苏科技大学 | Array type constant temperature control device for detecting new corona RNA viruses |
| US11607682B2 (en) | 2019-01-15 | 2023-03-21 | Beijing Boe Technology Development Co., Ltd. | Detection chip, using method for the same, and reaction system |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1584043A (en) * | 2004-05-27 | 2005-02-23 | 上海交通大学 | Spiral microchannel polymerase chain reacting chip |
| CN1804043A (en) * | 2005-01-14 | 2006-07-19 | 北京大学 | PCR chip micro-system and method for preparing the same |
-
2009
- 2009-11-25 CN CN2009102415361A patent/CN102071136A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1584043A (en) * | 2004-05-27 | 2005-02-23 | 上海交通大学 | Spiral microchannel polymerase chain reacting chip |
| CN1804043A (en) * | 2005-01-14 | 2006-07-19 | 北京大学 | PCR chip micro-system and method for preparing the same |
Non-Patent Citations (3)
| Title |
|---|
| 丁国杰等: "基于MEMS技术的PCR芯片及PDMS微腔阵列的设计", 《仪表技术与传感器》 * |
| 徐静平等: "PCR芯片控温系统", 《华中科技大学学报》 * |
| 赵湛等: "基于MEMS技术的PCR生物芯片的研究", 《传感器技术》 * |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559482B (en) * | 2011-12-31 | 2013-07-10 | 中山大学达安基因股份有限公司 | Gene detection device |
| CN102559482A (en) * | 2011-12-31 | 2012-07-11 | 中山大学达安基因股份有限公司 | Gene detection device |
| CN106872542A (en) * | 2015-12-13 | 2017-06-20 | 安徽中医药高等专科学校 | The preparation of detachable single heat or cold, hot two-purpose electrode |
| CN107656562A (en) * | 2016-07-26 | 2018-02-02 | 中国航空工业集团公司西安飞行自动控制研究所 | A kind of temperature control system for MEMS |
| CN106337021A (en) * | 2016-08-23 | 2017-01-18 | 上海交通大学 | Large-area accurate temperature control system for polymerase chain reaction |
| CN106929408A (en) * | 2017-01-26 | 2017-07-07 | 深圳市尚维高科有限公司 | Portable micro-fluidic PCR instrument and gene samples fluorescence quantitative detecting method |
| CN106929408B (en) * | 2017-01-26 | 2018-07-20 | 深圳市尚维高科有限公司 | Portable micro-fluidic PCR instrument and gene samples fluorescence quantitative detecting method |
| CN107129933A (en) * | 2017-05-08 | 2017-09-05 | 大连大学 | One kind is based on electrically driven (operated) digital microcurrent-controlled pcr chip device |
| CN109870205A (en) * | 2017-12-04 | 2019-06-11 | 上海新微技术研发中心有限公司 | Microfluidic flow meter and manufacturing method thereof |
| CN109870205B (en) * | 2017-12-04 | 2021-01-26 | 上海傲睿科技有限公司 | Microfluidic flow meter and manufacturing method thereof |
| US11607682B2 (en) | 2019-01-15 | 2023-03-21 | Beijing Boe Technology Development Co., Ltd. | Detection chip, using method for the same, and reaction system |
| CN112020551A (en) * | 2019-03-29 | 2020-12-01 | 京东方科技集团股份有限公司 | Detection chip, use method thereof and reaction system |
| CN112020551B (en) * | 2019-03-29 | 2022-11-25 | 京东方科技集团股份有限公司 | Detection chip, use method thereof and reaction system |
| US11654435B2 (en) | 2019-03-29 | 2023-05-23 | Boe Technology Group Co., Ltd. | Detection chip, method for operating detection chip, and reaction system |
| CN113106011A (en) * | 2021-03-29 | 2021-07-13 | 江苏科技大学 | Array type constant temperature control device for detecting new corona RNA viruses |
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Application publication date: 20110525 |