CN102068537A - Production method for preparing health care foods and medicaments for preventing and treating nasopharyngeal darcinoma by using tangerine peel and liquoric root - Google Patents
Production method for preparing health care foods and medicaments for preventing and treating nasopharyngeal darcinoma by using tangerine peel and liquoric root Download PDFInfo
- Publication number
- CN102068537A CN102068537A CN2011100204132A CN201110020413A CN102068537A CN 102068537 A CN102068537 A CN 102068537A CN 2011100204132 A CN2011100204132 A CN 2011100204132A CN 201110020413 A CN201110020413 A CN 201110020413A CN 102068537 A CN102068537 A CN 102068537A
- Authority
- CN
- China
- Prior art keywords
- serum
- deactivation
- health care
- pastille
- preventing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a production method of health care foods and medicaments for preventing and treating nasopharyngeal darcinoma, which are prepared by traditional Chinese medicines of tangerine peel and liquoric root. Health care buccal tablets which are prepared by taking the tangerine peel and the liquoric root as active ingredients according to the method, mainly used for eliminating inflammation and relieving itching and applied for protecting throat and preventing hoarseness, have been widely applied in sanitation and health care for the inflammation of the throat; and the latest study finds that an extract prepared by the method has a very obvious inhibition role for cells of the nasopharyngeal darcinoma, the serum pharmacology analysis particularly proves that active ingredients of a formula constituted by the tangerine peel and the liquoric root can enter into a human body through serum or plasma, have the obvious inhibition role for the growth of the cells of the nasopharyngeal darcinoma and be used for prevention and auxiliary treatment for patients with the nasopharyngeal darcinoma, and a tangerine peel and liquoric root mixture prepared by the production process can be prepared into the pharmaceutical preparations and the health care foods for preventing and treating the nasopharyngeal darcinoma through the traditional production process.
Description
Technical field the present invention relates to the health food of preventing and treating nasopharyngeal carcinoma made with Chinese medicine Pericarpium Citri Reticulatae Radix Glycyrrhizae and the production method of medicine.
Background technology
Nasopharyngeal carcinoma is the malignant tumor that betides mucous membrane of nasopharynx, and sickness rate is southern higher with China, and ground such as Guangdong, Guangxi, Fujian, Hunan are multiple district, and the man is more than the woman.Age of onset mostly is middle age greatly, also has teenager to suffer from the patient.Particularly Zhaoqing, Foshan and the area, Guangzhou in the middle part in Guangdong and western part are higher.Four of Zhaoqing can the sickness rate male be 25.12/10 ten thousand, and the women is 12.11/10 ten thousand; The male of Zhongshan city is 21.73/10 ten thousand, and the women is 8.66/10 ten thousand; Hong Kong male is 24.3/10 ten thousand, and the women is 10.2/10 ten thousand.The nasopharyngeal carcinoma cause of disease is relevant with ethnic susceptibility (yellow is ill many than white people), inherited genetic factors and ebv infection etc., and the nasopharyngeal carcinoma grade malignancy is higher, can occur cervical lymph node in early days and shift.It is reported that live in the male of the local language in middle part, Guangdong Province and behaviour Guangdong, its sickness rate is 30～50,/10 ten thousand.With regard to the whole nation, the sickness rate of nasopharyngeal carcinoma is reduced to north gradually by south, is not higher than 2～3/,100,000 as the most northern sickness rate.
Studies show that the generation of ebv infection and nasopharyngeal carcinoma is closely related, the existence of Epstein-Barr virus is all arranged at most of keratinization squamous cell carcinoma and nearly all undifferentiated squamous cell carcinoma.Whole world most people all infected Epstein-Barr virus in the Childhood, but the generation of nasopharyngeal carcinoma is arranged after having only a minority to grow up.The Epstein-Barr virus pathogenesis still imperfectly understands.The mechanism that body immune system is escaped immune response to the control action and the Epstein-Barr virus of ebv infection is the emphasis of research.
Radix Glycyrrhizae is as ancient simply plant amedica, and is how on the books in the ancient medicine ancient books and records of China, India and Greece, has use history in several thousand so far, the clinical multiple diseases such as respiratory system, digestive system, immune system that are used for the treatment of.Pharmacological research shows various active such as Radix Glycyrrhizae and extract thereof have antibiotic, antiviral, antiinflammatory, anticancer, antioxidation, protect the liver, neuroprotective, blood sugar lowering, memory reinforcing; antiviral activity about Radix Glycyrrhizae; but reported the propagation of multiple viruses such as the external HIV (human immunodeficiency virus) inhibiting of Radix Glycyrrhizae (HIV), severe acute respiratory syndrome virus (SARS), respiration system virus, vesicular stomatitis virus, but do not had evidence proof Radix Glycyrrhizae or its extract that Epstein-Barr virus is had inhibitory action so far.At anticancer aspect, reported that Radix Glycyrrhizae extract can directly effectively suppress the growth and the cell proliferation of breast carcinoma, Ehrlich tumor, EAT, carcinoma of endometrium and multiple solid tumor, can also effectively suppress the transfer of pulmonary carcinoma simultaneously.Radix Glycyrrhizae and cisplatin combined taking commonly used in addition can also reduce the oxidative stress of cisplatin induction, reduce its toxic and side effects, strengthen the effect of cancer chemotherapy.But the effect that Radix Glycyrrhizae is used nasopharyngeal carcinoma does not separately also appear in the newspapers at present.
Pericarpium Citri Reticulatae is the mature peel of rutaceae orange and variety thereof.The merit of appetizing in tool regulating QI and lowering the adverseness, the accent, drying dampness to eliminate phlegm.Clinical dirty contrary, the difficulty in urination and defecation of full vexed, the abdominal distention of the wet resistance of stagnation of QI in spleen and stomach, chest and diaphragm, anorexia, vomiting, lung qi retardance, the cough with copious phlegm of being used for the treatment of, also control acute mastitis from the beginning of etc.Pharmacological research proves that Pericarpium Citri Reticulatae has gentle stimulation to gastrointestinal tract, but pneumatosis in the intestinal tube is got rid of in the secretion of facilitating digestion liquid; Can be excited myocardium, occur on the contrary when dosage is excessive suppressing.It also can make blood vessel produce slight contraction, and the blood pressure that raises rapidly also has certain preventive effect to the arteriosclerosis that high fat diet causes, Pericarpium Citri Reticulatae also has phlegm-dispelling functions and antiinflammatory action.The compound preparation that contains Pericarpium Citri Reticulatae has antivirus action, but list is not also seen bibliographical information with the antivirus action of Pericarpium Citri Reticulatae.The existing report of compound preparation antitumor action that contains Pericarpium Citri Reticulatae, but list does not see also that with the antitumor action of Pericarpium Citri Reticulatae the effect of bibliographical information, particularly anti-nasopharyngeal carcinoma does not also have bibliographical information at present.
This project team finds, with the Pericarpium Citri Reticulatae Radix Glycyrrhizae is that a kind of edible health sugar that raw material is made has very obvious suppression effect to nasopharyngeal carcinoma cell, analysis by serum pharmacological, prove that also prescription that the Pericarpium Citri Reticulatae Radix Glycyrrhizae is formed has the activity of important anti-nasopharyngeal carcinoma, can make pharmaceutical preparation and the health food of preventing and treating nasopharyngeal carcinoma by special production technology.
Summary of the invention a kind of with the Pericarpium Citri Reticulatae Radix Glycyrrhizae be raw material make have the prevention and the treatment nasopharyngeal cancer function preparation and health food, this product can be made by following production method: 3.5 parts of extracting liquorices, Pericarpium Citri Reticulatae decoct with water three times for 1 part, 2.5 hours for the first time, 1.5 hours for the second time, 1 hour for the third time, filter, merging filtrate, filtrate decompression concentrate extractum.Get 1 part of extractum, 4 parts of sucrose, 1 part in dextrin and ethanol are an amount of, granulate, and drying adds Borneolum Syntheticum (dissolving in right amount with ethanol earlier) mixing, and tabletting is promptly.This method with the Pericarpium Citri Reticulatae Radix Glycyrrhizae be raw material make a kind of based on anti-inflammatory anti-itch, be applied to protect throat, prevent cracked health care buccal tablets, be widely used in the health care of laryngopharyngitis; Current research is found, extractum with this method preparation has very obvious suppression effect to nasopharyngeal carcinoma cell, the particularly analysis by serum pharmacological, the effective ingredient of the prescription that proof Pericarpium Citri Reticulatae Radix Glycyrrhizae is formed can enter in the body by serum or blood plasma, growth to nasopharyngeal carcinoma cell has the obvious suppression effect, the prevention and the auxiliary treatment that can be used for the nasopharyngeal carcinoma patient, the Pericarpium Citri Reticulatae licorice mixture of this production technology preparation can be made pharmaceutical preparation and the health food of preventing and treating nasopharyngeal carcinoma by traditional production technology.
Pericarpium Citri Reticulatae, Radix Glycyrrhizae lung moistening, expelling phlegm for arresting cough, regulating qi-flowing for strengthening spleen, drying dampness to eliminate phlegm, heat-clearing and toxic substances removing are principal agent in this formula for a product, are aided with Borneolum Syntheticum clearing away heat and nourishing YIN, cooling blood and removing stasis to help the merit of principal agent resolving toxin and disinhibiting the throat.The main component of Radix Glycyrrhizae, Pericarpium Citri Reticulatae is a water soluble ingredient, so the chip select agent, meets the medication custom of Chinese medicine as the dosage form of this prescription, medicine easily is absorbed, and the curative effect height is rapid-action, meets the clinical suitable card of this product.And after making tablet, be convenient to store, carry, quality is more stable.
Contained chemical constituent is Hesperidin, Nobiletin, limonene, a-pinene, B-pinene, B-phellandrene etc. in the Pericarpium Citri Reticulatae, and contains various trace elements.Its main pharmacodynamics composition is that Hesperidin is soluble in water, methanol, the atomic ethyl acetate that is dissolved in of ethanol.Contained chemical constituent is glycyrrhizic acid, Glycyrrhiza uralensis Fisch. saponin first, glycyrrhizin, isoliquiritigenin, glycycoumarin, isoglycycoumarin, licochalcone, glycyol, isoglycyol etc. in the Radix Glycyrrhizae, wherein glycyrrhizic acid is white or faint yellow crystal type powder, fusing point is 220 ℃, special sweet taste is arranged, its sugariness is about 250 times of sucrose, be dissolved in the Diluted Alcohol of hot water and heat, be insoluble to dehydrated alcohol and ether.
The specific embodiment: embodiment one
The Pericarpium Citri Reticulatae glycyrrhiza preparation suppresses the research of nasopharyngeal carcinoma cell CNE-1 propagation
1. material and method
1.1 material
1.1.1 key instrument
High speed low temperature centrifugal machine U.S. SIGMA
Superclean bench Beijing semiconductor equipment one factory
CO2 constant temperature incubator France Jouan
1.1.2 main agents
Trypsin U.S. SIGMA
Tetrazolium bromide U.S. SIGMA
Dimethyl sulfoxide Beijing northization fine chemistry company
1.1.3 reagent preparation
The preparation of MTT solution: take by weighing MTT 0.5 gram, be dissolved in the phosphate buffer (PBS) of 100ml, to remove the antibacterial in the solution, put 4 ℃ and keep in Dark Place and get final product with 0.22 μ m membrane filtration.
The preparation of PBS solution: Nacl 8g+Kcl 0.2g+Na2HPO4 1.44g+KH2PO4 0.24g transfers pH 7.4, standardize solution 1L.。
1.2 experimental technique
1.2.1 medication preparation and dosage
The Pericarpium Citri Reticulatae glycyrrhiza preparation is prepared from by this patent invention part producing.During experiment above-mentioned tablet put to decoct in the container and dissolve, be made into the decocting liquid that contains crude drug amount 50mg/ml and irritate stomach.According to " humans and animals body surface area conversion dose,equivalent ratio table ", this is tested selected concrete dosage and is: rabbit dosage (g/kg.d)=day for human beings is with dosage * 0.07/1.5 (g/kg.d).
1.2.2 the preparation of pastille serum, blood plasma
Healthy 16 of new zealand white rabbits (Guangdong Medical College's Experimental Animal Center provides), female, body weight 1.8kg～2.0kg.Be divided into four groups at random: blank serum matched group, blank plasma matched group, Pericarpium Citri Reticulatae glycyrrhiza preparation serum group, Pericarpium Citri Reticulatae glycyrrhiza preparation blood plasma group.Fasting 10～12h before the administration can't help water.Each organize rabbit in every day gastric infusion once, dosage according to the weight at every turn, successive administration 10d, 1h after last 1 perfusion, carotid artery fruit blood under the aseptic condition, blood sampling divides four parts: normal serum, inactivated serum, normal plasma, deactivation blood plasma.
Normal serum fruit blood process: carotid artery blood sampling under the aseptic condition, room temperature is placed 2h, then after 4 ℃ of refrigerator overnight, centrifugal (2500rmin
-1) separation of serum, it is transparent, limpid, faint yellow that serum is, and do not have precipitation, haemolysis not, and packing is put-70 ℃ of refrigerators and is preserved, and uses in the week.
The preparation process of inactivated serum: get the normal serum of packing, 56 ℃, 30min after the deactivation, puts-70 ℃ of refrigerators and preserves, and uses in the week.
The normal plasma blood collection procedure: carotid artery blood sampling under the aseptic condition, fire in the anticoagulant tube of sour sodium anticoagulant centrifugal immediately (2500rmin in adding an amount of Chinese holly
-1), separated plasma, packing is put-70 ℃ of refrigerators and is preserved, and uses in the week.
The preparation process of deactivation blood plasma: get the normal plasma of packing, 56 ℃, 30min after the deactivation, puts-70 ℃ of refrigerators and preserves, and uses in the week.
1.2.3 the evaluation of pastille serum, blood plasma
Getting Pericarpium Citri Reticulatae glycyrrhiza preparation pastille serum, pastille blood plasma, deactivation pastille serum, deactivation pastille blood plasma, blank rabbit anteserum, blank rabbit plasma 1ml respectively puts in the 10ml volumetric flask, add methanol to scale, protein precipitation, behind the ultrasonic 30min, 4 ℃ of refrigerators are placed and are spent the night, get supernatant and cross 0.45 μ m filter membrane, sample introduction.Identify having or not of Hesperidin and glycyrrhizic acid, administration group as a result can not detect in the inactivated serum and contain glycyrrhizic acid composition (T=22.443min), can detect in the administration group deactivation blood plasma and contain Hesperidin composition (T=6.075min), and the blank group does not all detect Hesperidin and glycyrrhizic acid composition, may with glycyrrhizic acid in pastille group serum, the blood plasma and content of hesperidin is low and metabolism is relevant soon.
1.2.4 the cultivation of KB cell
The CNE-1 cell strain is with containing 10% hot deactivation fresh calf serum in 37 ℃, 5%CO
2Cultivate in the incubator.Eugonic cell went down to posterity once in per 2 two days.
1.2.5MTT method detects the growth of cancer cells suppression ratio
Use trypsin digestion cell, be made into the cell suspension kind with conventional culture medium and go into 96 orifice plates, the hole of plate periphery does not add cell, has prevented edge effect.Blank hole zeroing does not add cell and makes blank only to add culture fluid.At 37 ℃, 5%CO
2Incubator in cultivate 24h after, add volume ratio respectively and be: 5%, 10%, 15%, 20%, 40% pastille serum, pastille blood plasma, control serum and contrast blood plasma.Establish 4 parallel holes for every group, continue to cultivate 6h, add MTT solution 20 μ L and continue to cultivate 4h, stop cultivating, abandon culture fluid, add dimethyl sulfoxide 150 μ L/ holes, each hole absorbance A value is measured in vibration on enzyme-linked immunosorbent assay instrument, return to zero with blank well during colorimetric.According to the above-mentioned A value of cultivating 12h, 18h, 24h, 48h that detects with quadrat method.Calculate inhibitory rate of cell growth according to formula: inhibitory rate of cell growth=(blank group A-experimental group A value)/blank group A value * 100%.
1.2.6 statistical procedures
Use the SPSS16.0 statistical software and carry out statistical analysis, the A value is used
The t check is adopted in expression; Suppression ratio adopts correlation analysis with time and concentration; Use the multifactor analysis of variance between group.
2 results
2.1MTT experimental result
2.2.1 variable concentrations is deactivation pastille MT in serum T result not
With volume ratio is that 5%, 10%, 15%, 20%, 40% Pericarpium Citri Reticulatae glycyrrhiza preparation pastille serum, absorbance and the suppression ratio that blood plasma acts on CNE-1 cell 6h, 12h, 18h, 24h, 48h respectively see Table 1～8.
Table 1 variable concentrations is deactivation pastille serum group absorbance not
Annotate: compare with matched group
△Compare with matched group P＜0.05
*P＜0.01
The not deactivation pastille serum of table 2 variable concentrations is to the suppression ratio of CNE-1 in different time points
Table 1 table 2 variable concentrations not deactivation gardener sugar pastille MT in serum T result shows: CNE-1 gives the pastille serum of 5%, 10%, 15%, 20%, 40% variable concentrations and the culture fluid of blank serum respectively.The result shows along with the rising suppression ratio of serum-concentration is in rising trend, and prolongation in time, and inhibitory action is more obvious.
2.2.2 variable concentrations is deactivation pastille blood plasma MTT result not
Table 3 variable concentrations is deactivation pastille blood plasma group absorbance not
Annotate: compare with matched group
△Compare with matched group P＜0.05
*P＜0.01
△
The not deactivation pastille blood plasma of table 4 variable concentrations is to the suppression ratio of CNE-1 in different time points
Table 3, table 4 display result shows: CNE-1 gives the not deactivation pastille blood plasma of 5%, 10%, 15%, 20%, 40% variable concentrations and the culture fluid of deactivation contrast blood plasma not respectively.The result shows along with the rising suppression ratio of deactivation pastille plasma concentration not is in rising trend, and prolongation in time, and inhibitory action is more obvious.But compare with deactivation pastille serum not, the suppression ratio of each level descends to some extent, may with blood plasma in contain certain principal component be not relevant.
2.2.3 variable concentrations deactivation gardener sugar pastille MT in serum T result
Table 5 variable concentrations deactivation pastille serum group absorbance
Annotate: compare with matched group
△Compare with matched group P＜0.05
*P＜0.01
The deactivation pastille serum of table 6 variable concentrations is to the suppression ratio of CNE-1 in different time points
Table 5, table 6 display result shows: CNE-1 gives the deactivation pastille serum of 5%, 10%, 15%, 20%, 40% variable concentrations and the culture fluid of deactivation control serum respectively.The result shows along with the rising suppression ratio of deactivation pastille serum-concentration is in rising trend, and prolongation in time, and inhibitory action is more obvious.But compare with the pastille serum of not deactivation, each horizontal suppression ratio downward trend is obvious.
2.2.4 variable concentrations deactivation gardener sugar pastille blood plasma MTT result
Table 7 variable concentrations deactivation blood plasma group absorbance
Annotate: compare with matched group
△Compare with matched group P＜0.05
*P＜0.01
The deactivation pastille blood plasma of table 8 variable concentrations is to the suppression ratio of CNE-1 in different time points
Table 7, table 8 display result shows: CNE-1 gives the deactivation pastille blood plasma of 5%, 10%, 15%, 20%, 40% variable concentrations and the culture fluid of deactivation contrast blood plasma respectively.The result shows along with the rising suppression ratio of plasma concentration is in rising trend, and prolongation in time, and inhibitory action is more obvious.But compare with the pastille blood plasma of not deactivation, each horizontal suppression ratio downward trend is obvious.Simultaneously, the suppression ratio of 40% concentration pastille blood plasma different time points descends obviously, may with deactivation after to have reduced cytotoxicity relevant, its concrete reason is still waiting further research.
3 discuss
The Pericarpium Citri Reticulatae glycyrrhiza preparation be professor Wu Tie of Guangdong Medical College to treat laryngopharyngeal diseases be main prescription, be make laws according to the pathological characteristic of " clear respectful energy of impairment of dispersing and descending function of the lung, the evil throat that gathers ", prescription, medication, diseases such as clinical treatment pharyngolaryngitis have obtained better curative effect.Chinese medicine is thought, disturbs the main cause into the nasopharyngeal carcinoma morbidity on lung-heat phlegm-fire and the liver and gall pyretic toxicity.Part of the body cavity above the diaphragm housing the heart and lungs thermal-arrest, Lung Qi obstraction, heat very forcing blood from through epistaxis occurring, coagulation of QI-blood then, Tianjin is poly-to be expectorant, stagnation of phlegm-heat forms lump; The liver failing to maintain the normal flow of QI, stagnation of QI diarrhea due to stagnation of QI, diarrhea due to stagnation of QI can not transmitting and distributing the fluidses, gather to be expectorant, and the turbid coagulation of expectorant forms node, lump; Hepatic depression is let out, strongly fragrant fire-transformation of a specified duration, and bright liquid is expectorant, disturbing clearly on the phlegm-fire, sucutaneous nodule appears in the then irritated irritability of sun, tinnitus, deafness, headache, blurred vision, cervical region.And the Pericarpium Citri Reticulatae glycyrrhiza preparation is a method of treatment with regulating qi-flowing for strengthening spleen, drying dampness to eliminate phlegm, heat-clearing and toxic substances removing, clearing away heat and nourishing YIN, cooling blood and removing stasis, has the probability of potential prevention and treatment nasopharyngeal carcinoma.The Chinese medicine compound majority works by oral, directly add the research that experimentizes in the reaction system that exsomatizes with Chinese medicine preparation, on methodology, there are a lot of problems, as influence of the impurity component in the Chinese medicine, various tannin and different pH values etc., all can affect to experimental result.The Chinese medicine serum pharmacological has realized the better combination of experiment in experiment in vitro and the body, on methodology, has novelty, can get rid of the influence of above various factors,, be a kind of comparatively ideal method of research pharmacology of Chinese materia medica at present relatively near the process that produces pharmacodynamics effect in the medicine internal milieu.But, recently domestic many scholars propose, blood plasma pharmacology and serum pharmacological have status of equal importance in pharmacology of Chinese materia medica research, propose to adopt the method that pastille serum and pastille blood plasma pharmacological method start at the same time to study, further confirmatory experiment result's reliability.In order to reduce immunoreation that causes because of species variation etc., laboratory animal should be selected for use and the proximate species of human biology's characteristic as far as possible, is first-selection with rabbit and rat.Consider plurality of enzymes, antibody, complement and other biological active substance contained in the serum of donor model or the blood plasma by exerting an influence to experiment, the interference experiment result is so give the pretreatment of 56 ℃ of 30min deactivations.But also there is the scholar to think it unnecessary to carry out pretreatment, because these are by animal pattern itself, or by the bioactive substance that medicine irritation produced, can produces certain effect, perhaps this mechanism of action place of medicine just] the cell of In vitro culture.So, for strictly according to the facts reaction experiment result's accuracy, the blank group of upright variable concentrations and with the two simultaneously under the same terms comparative analysis get rid of the non-medication influence.Someone proposes the general dosage regimen (leading to method) of Chinese medicine serum pharmacological research and provides the increase dosage diluted partly to remedy pastille serum, the dose of pastille serum during the actual adding of control is preferably reacted and is.
Representative effective ingredient in the Pericarpium Citri Reticulatae glycyrrhiza preparation of the trace that may contain in HPLC detection pastille serum and the blood plasma, success with the making of checking serum and blood plasma, HPLC is simple to operate, and is highly sensitive, can well carry out qualitative evaluation to pastille serum and blood plasma.
The MTT development process is to estimate one of method that the tumor cell survival condition praised highly.When tumor cell obvious profile variation do not occur as yet under light microscopic, the reducible MTT of succinate dehydrogenase in the mitochondrion of its living cells and form the bluish violet crystal of indissoluble, dead cell does not then have this function.Measure its absorption value in the cell scope of some with enzyme-linked immunosorbent assay instrument, reflect the quantity of living cells indirectly.Mtt assay reflects the sensitivity differences of different tumor cells to various antitumor drug, and with other susceptibility method and clinical efficacy good dependency is arranged all, has important value for clinical rational drug use, realization tumour patient individualized treatment.
This experiment utilizes serum and blood plasma pharmacological method that systematic research has been carried out in the effect of Pericarpium Citri Reticulatae glycyrrhiza preparation prevention nasopharyngeal carcinoma morbidity on existing research basis.Select for use new zealand white rabbit to prepare gardener's sugar pastille serum, press logical method scheme with the clinical equivalent dosage of rabbit, irritate stomach 10d to rabbit, once-a-day, prepare concentration then and be 5%, 10%, 15%, 20%, 40% variable concentrations pastille serum and blood plasma and carry out not deactivation and inactivation treatment.The result shows: along with the prolongation of time and the increase of concentration, not deactivation pastille serum, deactivation pastille blood plasma, deactivation pastille serum, deactivation pastille blood plasma do not show a rising trend to CNE-1 cell inhibiting rate, present good amount-effect, time-effect relationship.Do not compare in twos between the group of deactivation and inactivation treatment method, there was no significant difference (P＞0.05) between the pastille blood plasma group of the pastille serum of deactivation and not deactivation not, there was no significant difference (P＞0.05) between deactivation pastille serum and deactivation pastille blood plasma group, do not have extremely significant difference (P＜0.01) between deactivation pastille serum and deactivation pastille serum group, do not have extremely significant difference (P＜0.01) between deactivation pastille blood plasma and deactivation pastille blood plasma group.Concentration is 20% not deactivation pastille serum, deactivation pastille blood plasma, deactivation pastille serum, deactivation pastille blood plasma do not compare CNE-1 with matched group separately and is significant difference (P＜0.05).The result shows that after the Pericarpium Citri Reticulatae glycyrrhiza preparation was oral, its effective ingredient can enter in blood plasma and the serum, and performance suppresses the effect of nasopharyngeal carcinoma cell growth in vivo.The Pericarpium Citri Reticulatae glycyrrhiza preparation can be used as a kind of health food that prevents nasopharyngeal carcinoma, also can be further production method by modern medicinal agents, preparation Pericarpium Citri Reticulatae Radix Glycyrrhizae extractum, and can be prepared into the pharmaceutical preparation that pill, tablet, granule, capsule, injection etc. can supply clinical practice respectively by traditional production technology, as the medicine of prevention and treatment nasopharyngeal carcinoma.
Claims (2)
1. a pharmaceutical preparation and production method of health food-Yizhibao that can be used for preventing nasopharyngeal carcinoma, the prescription that it is characterized in that this production method is made up of Pericarpium Citri Reticulatae and Radix Glycyrrhizae, can be product that can be edible by following prepared: 3.5 parts of extracting liquorices, Pericarpium Citri Reticulatae decoct with water three times for 1 part, 2.5 hours for the first time, 1.5 hours for the second time, 1 hour for the third time, filter, merging filtrate, filtrate decompression concentrate extractum.Get 1 part of extractum, 4 parts of sucrose, 1 part in dextrin and ethanol are an amount of, granulate, and drying adds Borneolum Syntheticum (dissolving in right amount with ethanol earlier) mixing, and tabletting is promptly.
2. a kind of pharmaceutical preparation and production method of health food-Yizhibao that can be used for preventing nasopharyngeal carcinoma as claimed in claim 1 is characterized in that the Pericarpium Citri Reticulatae Radix Glycyrrhizae extractum of this production method preparation can be prepared into pill, tablet, granule, capsule, injection respectively by traditional production technology.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110020413.2A CN102068537B (en) | 2011-01-06 | 2011-01-06 | The preparation of Pericarpium Citri Reticulatae Radix Glycyrrhizae prevents and treats the health food of nasopharyngeal carcinoma and the production method of medicine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110020413.2A CN102068537B (en) | 2011-01-06 | 2011-01-06 | The preparation of Pericarpium Citri Reticulatae Radix Glycyrrhizae prevents and treats the health food of nasopharyngeal carcinoma and the production method of medicine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102068537A true CN102068537A (en) | 2011-05-25 |
| CN102068537B CN102068537B (en) | 2015-12-02 |
Family
ID=44027415
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110020413.2A Expired - Fee Related CN102068537B (en) | 2011-01-06 | 2011-01-06 | The preparation of Pericarpium Citri Reticulatae Radix Glycyrrhizae prevents and treats the health food of nasopharyngeal carcinoma and the production method of medicine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102068537B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105250646A (en) * | 2015-11-23 | 2016-01-20 | 李先强 | Medicine for treating pharyngeal cancer |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1104458A (en) * | 1994-06-16 | 1995-07-05 | 谭灿文 | Complex amino acids instant drink |
| CN1163729A (en) * | 1997-04-21 | 1997-11-05 | 沈文湘 | Natural bamboo juice product and its making method |
| CN1492737A (en) * | 2001-02-21 | 2004-04-28 | 郑钟文 | Functional agent for decomposing nicotine and method of preparing the same |
| CN1781513A (en) * | 2004-12-02 | 2006-06-07 | 黄荷生 | Lozenge for choke |
-
2011
- 2011-01-06 CN CN201110020413.2A patent/CN102068537B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1104458A (en) * | 1994-06-16 | 1995-07-05 | 谭灿文 | Complex amino acids instant drink |
| CN1163729A (en) * | 1997-04-21 | 1997-11-05 | 沈文湘 | Natural bamboo juice product and its making method |
| CN1492737A (en) * | 2001-02-21 | 2004-04-28 | 郑钟文 | Functional agent for decomposing nicotine and method of preparing the same |
| CN1781513A (en) * | 2004-12-02 | 2006-06-07 | 黄荷生 | Lozenge for choke |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105250646A (en) * | 2015-11-23 | 2016-01-20 | 李先强 | Medicine for treating pharyngeal cancer |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102068537B (en) | 2015-12-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101474375B (en) | Compound Cordyceps militaris L. Link preparation and production method and application | |
| CN102085248B (en) | Traditional Chinese medicinal composition for treating cervix diseases, method for preparing same and method for detecting same | |
| CN101711848B (en) | Chinese medicinal composition capable of adjunctively treating tumour | |
| CN101708306B (en) | Chinese medicinal composition for treating gastropathy and preparation method thereof | |
| CN108813610A (en) | A kind of saussurea involucrata composition and its application for improving immunity | |
| CN104382985A (en) | Pharmaceutical composition for preventing and treating non-alcoholic fatty liver disease | |
| CN102068475B (en) | Application and blood-sugar lowering effective part of trapa acornis nakano shell as well as extraction method of blood-sugar lowering effective part | |
| CN101120977B (en) | Medicine for treating tumor | |
| CN101007052B (en) | An antitumor medicine and its preparation method | |
| CN102068537B (en) | The preparation of Pericarpium Citri Reticulatae Radix Glycyrrhizae prevents and treats the health food of nasopharyngeal carcinoma and the production method of medicine | |
| CN108542927A (en) | The application of the obcordate aspidopterys stem of falling the heart and its extract in anti-tumor aspect | |
| CN101948453A (en) | Novel NEO-clerodane typed diterpene compound and application thereof | |
| CN101147785A (en) | Medicinal preparation for preventing tumor | |
| CN104027447B (en) | A kind of Chinese medicine of Therapeutic cancer | |
| CN102805799A (en) | Traditional Chinese medicine composition with antitumous effect | |
| CN105708845B (en) | A kind of application of phenylpropanoids and its pharmaceutically acceptable salt in the drug for preparing treatment diseases associated with inflammation | |
| CN106074579B (en) | A kind of application of phenylpropanoids in the drug for preparing treatment diseases associated with inflammation | |
| CN105521327B (en) | Medicinal composition for preventing and/or curinng alcoholic liver disease or liver cancer | |
| CN104644661A (en) | Application of 20(R)-ginsenoside Rg3 in preparation of medicament for relieving or/and treating hepatitis B and medicament | |
| CN113101331B (en) | Thyme herb tea and preparation method and application thereof | |
| CN116849357B (en) | Medicinal and edible composition with liver protection function and preparation method thereof | |
| CN106074583B (en) | A kind of application of phenylpropanoids and its pharmaceutically acceptable salt in the drug for preparing treatment diseases associated with inflammation | |
| CN101069703A (en) | Medicine for treating acute, chronic pharyngitis, acute, chronic tonsillitis and preparing method | |
| CN101766675A (en) | Preparation method of silktree albizia bark extract for inhibiting angiogenesis and application | |
| CN105669792B (en) | A kind of preparation method of phenylpropanoids |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151202 Termination date: 20200106 |