CN102068401B - Tuber fleeceflower root hair blackening formula and preparation method thereof - Google Patents
Tuber fleeceflower root hair blackening formula and preparation method thereof Download PDFInfo
- Publication number
- CN102068401B CN102068401B CN201110007820XA CN201110007820A CN102068401B CN 102068401 B CN102068401 B CN 102068401B CN 201110007820X A CN201110007820X A CN 201110007820XA CN 201110007820 A CN201110007820 A CN 201110007820A CN 102068401 B CN102068401 B CN 102068401B
- Authority
- CN
- China
- Prior art keywords
- parts
- hair
- polygoni multiflori
- radix polygoni
- herba
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a tuber fleeceflower root hair blackening formula, which comprises the following Chinese medicinal compositions in part by weight: 1 to 40 parts of tuber fleeceflower root, 1 to 40 parts of black sesame, 1 to 40 parts of rhizoma drynariae, 1 to 40 parts of yerbadetajo herba ecliptae, 1 to 30 parts of glossy privet fruit, 1 to 30 parts of south dodder seed, 1 to 30 parts of prunella spike, 1 to 30 parts of hiraute shiny bugleweed herb, 1 to 30 parts of mint, 1 to 30 parts of szechuan lovage rhizome and 1 to 30 parts of dried ginger. Two external formulations such as a lotion and a liniment/tincture can be prepared by adding a certain amount of water into the tuber fleeceflower root hair blackening formula or extracting with ethanol. Through the pathogenic wind dispersing, tonifying and blood circulation invigorating effects of the tuber fleeceflower root hair blackening formula, the activity of tyrosinase in hair follicles is enhanced, the synthesis of melanin is promoted for blackening hair and poliosis is improved. A plurality of raw materials are used together, so that blood circulation of scalp is promoted, the scalp, hair and hair follicle obtain sufficient nutrition, the regeneration of hair melanin cells is promoted, and white hairs becomes black from roots. Moreover, the tuber fleeceflower root hair blackening formula has the effects of removing scurf, stopping itching in head, washing hair and protecting hair.
Description
Technical field
The invention belongs to cosmetic field, disclose a kind of Radix Polygoni Multiflori hair color side that is used to improve poliosis and preparation method thereof.
Background technology
Raising along with health of masses consciousness; People hope to have more hair blackening product to select; It is not only demand to the function sectionalization; And hope natural sex, the mildness and professional of product, from the fashionable market of skin care item of plant, draft, Chinese medicine class, " healthy, green, natural " is the standard that people define quality of the life.The Chinese medicine hair blackening product has met people's demand undoubtedly.In the crowd, hoary-headed incidence rate is very high, accounts for 20%-30%, and the middle and upper-class people is especially obvious, accounts for 60%.This is comprising " teen-ager leukotrichia ", the gray hair of the middle aged 30-69 year " poliosis " of juvenile poliosis below 29 years old.The people of ageing, it is very common problem that hair bleaches as an irreversible process as far as just.Most of Asians hopes that hair is pitch-black moist, and therefore businessman all catches Asians's this tradition psychology both at home and abroad, releases various black hair hair color products, and the product that bleaches of this type control hair accounts for the very great share of hair care product cosmetics.
See from the demand aspect of shampoo brand advertising that at first the theme that hair is moistened in black hair and conditioning becomes the main flow of product, pitch-black naturally as having added in Rejoice a softer and smoother touch; Contain the hair care Chinese herbal medicine Radix Polygoni Multiflori that Chinese used thousands of years and moisten beautiful product; The good gentle of Guangzhou refined naturally fine chemistry industry Industrial Co., Ltd. is master's black essence with the Chinese medicine Radix Polygoni Multiflori, contains the Radix Polygoni Multiflori and moistens economic benefits and social benefits bioactive molecule of hair color or the like.
Secondly; Classification from hair blackening product; Mainly contain following two kinds: 1. exogenous control: comprise the hair dyeing goods of oxidized form hair dyeing method (being Permanent Hair Dyeing Agent, present modal hair colouring methods), imitation melanin generative process and set up forming melanic hair colouring methods on the DOPA.These several kinds of hair dyes can make the tone of hair that long persistency is arranged, and can dye various tones from shallow to deep, and are easy to use.2. endogenous control: promptly go deep into hair follicle and activate the enzyme in the melanocyte, make self to generate black black hair usually through activator.
In realizing process of the present invention; The inventor finds that there is following problem at least in prior art: at present, the hair dyeing product on the market is to reach the purpose of black hair through the hair that has grown out is dyeed basically, its shortcoming be permanent be not definitely persistent; Hair is with the speed growth of every month 12mm; Although being dyed part hair tone can not change very fast, new longer hair and remain poliosis, its permanent persistency that can only guarantee to be dyed part hair tone; And great majority use the dyestuff intermediate of phenyl amines, and its zest and toxicity belong to higher in cosmetic material.Not having a kind of in the market as yet is that special specific aim improves tryrosinase and increases the synthetic Chinese medicine hair blackening product of melanin through the endogenous control.
Summary of the invention
The purpose of the embodiment of the invention is the defective to above-mentioned prior art, provides a kind of activity that improves tyrosine acid enzyme in the hair follicle and melanin formation promoting to reach the Radix Polygoni Multiflori hair color side of pitch-black hair.
Another purpose of the embodiment of the invention provides the pharmaceutical composition that the above-mentioned Chinese medicine composition that contains effective dose and pharmaceutical carrier are formed.
Another purpose of the embodiment of the invention provides this preparation of drug combination method.
The technical scheme taked of the present invention is to achieve these goals:
A kind of Radix Polygoni Multiflori hair color side comprises following parts by weight of Chinese traditional medicine compositions:
1～40 part of Radix Polygoni Multiflori, 1～40 part of Semen Sesami Nigrum, 1～40 part of Rhizoma Drynariae, 1～40 part of Herba Ecliptae, 1～30 part of Fructus Ligustri Lucidi, 1～30 part of Semen Cuscutae, 1～30 part of Spica Prunellae, 1～30 part of Herba Lycopi, 1～30 part of Herba Menthae, 1～30 part of Rhizoma Chuanxiong, 1～30 part of Rhizoma Zingiberis.
The present invention provides optimized technical scheme to be: a kind of Radix Polygoni Multiflori hair color side comprises following parts by weight of Chinese traditional medicine compositions:
10～35 parts of Radix Polygoni Multiflori, 10～35 parts of Semen Sesami Nigrums, 10～30 parts of Rhizoma Drynariae, 10～30 parts of Herba Ecliptaes, 5～20 parts of Fructus Ligustri Lucidi, 5～20 parts of Semen Cuscutae, 5～20 parts of Spica Prunellaes, 5～20 parts of Herba Lycopi, 5～20 parts of Herba Menthaes, 5～20 parts of Rhizoma Chuanxiongs, 5～20 parts of Rhizoma Zingiberiss.
The present invention provides preferred technical scheme to be: a kind of Radix Polygoni Multiflori hair color side comprises following parts by weight of Chinese traditional medicine compositions:
20 parts of Radix Polygoni Multiflori, 20 parts of Semen Sesami Nigrums, 15 parts of Rhizoma Drynariae, 15 parts of Herba Ecliptaes, 15 parts of Fructus Ligustri Lucidi, 15 parts of Semen Cuscutae, 15 parts of Spica Prunellaes, 15 parts of Herba Lycopi, 10 parts of Herba Menthaes, 10 parts of Rhizoma Chuanxiongs, 10 parts of Rhizoma Zingiberiss.
The present invention also provides a kind of pharmaceutical composition, contains the above-mentioned Chinese medicine composition and the pharmaceutical carrier of effective dose.
Preparation of drug combination method of the present invention may further comprise the steps:
The method for preparing of lotion: get Radix Polygoni Multiflori, Semen Sesami Nigrum, Rhizoma Drynariae, Herba Ecliptae, Fructus Ligustri Lucidi, Semen Cuscutae, Spica Prunellae, Herba Lycopi, Herba Menthae, Rhizoma Chuanxiong and Rhizoma Zingiberis and mix, behind the distilled water merceration 20-40min that adding mixed medicinal materials gross weight 5-7 doubly measures in the mixed medicinal materials, heating; Boil the back and keep boiling 40min-80min, filter, give to add the distilled water that mixed medicinal materials gross weight 3-5 doubly measures in the medicinal residues; Boil the back and keep boiling 20-40min, filter, merge filtrating twice; Leave standstill 36-60h; Get supernatant,, promptly get lotion with citric acid adjust pH 6.0-6.5.
The method for preparing of liniment/tincture: get Radix Polygoni Multiflori, Semen Sesami Nigrum, Rhizoma Drynariae, Herba Ecliptae, Fructus Ligustri Lucidi, Semen Cuscutae, Spica Prunellae, Herba Lycopi, Herba Menthae, Rhizoma Chuanxiong and Rhizoma Zingiberis and mix, doubly measure 75% ethanol, airtight immersion 20-40min under the room temperature to adding medical material gross weight 5-7 in the mixed medicinal materials; 85 ℃ of heating and refluxing extraction twice after boiling for the first time, keep boiling 20-60min; Filtration back medicinal residues reuse mixed medicinal materials gross weight 3-5 doubly measures 75% ethanol and boils, and keeps boiling 20-40min, filters; Merge filtrating twice, leave standstill 36-60h, get supernatant; With citric acid adjust pH 6.0-6.5, promptly get liniment/tincture.
The beneficial effect of the embodiment of the invention is:
Radix Polygoni Multiflori hair color square tube of the present invention is crossed and is dispeled the wind, tonification, the effect of invigorating blood circulation, and improves to activate that tyrosinase activity and melanin formation promoting improve early whitening of beard and hair to reach pitch-black hair in the hair follicle; Many medicines share; Play promotion scalp blood circulation jointly, let scalp, hair and hair follicle obtain prescribing adequate nutrition, promote the regeneration of hair melanocyte; Thereby poliosis is turned black from root, and the effect of dandruff dispelling, stopping head itching, hair washing, hair care is arranged.The present invention mainly is the poliosis patient who does not freely cause qi depression to blood stasis to owing to QI-blood circulation.
The medicinal function of each medicine of the present invention and effect:
Radix Polygoni Multiflori: property is bitter, sweet, puckery, the flavor temperature.The liver benefiting that nourishes blood, black beard and hair dispels the wind." book on Chinese herbal medicine is looked for the truth " called its " grow the water the kidney invigorating, hair color is made light of one's life by commiting suicide ".Modern medicine confirms that Radix Polygoni Multiflori scalable neural and endocrine function and hairy root nourishing can impel hair melanin to generate, and make the hair blackening.
Semen Sesami Nigrum: property is sweet, flat.The function invigorating the liver and kidney, benefiting essence-blood, black beard and hair is used to treat dizziness and blurred vision, early whitening of beard and hair, post disease alopecia.Modern study shows that it has the particulate ability of the female pigment cell's synthetic dyestuff of the hair of enhancing, regulates chromatophorous metabolism, accelerates the effect of chromatophorous generation.
Rhizoma Drynariae: bitter in the mouth, warm in nature.The function nourishing liver and kidney, promoting blood circulation and stopping pain, growing and blacking hair.The external curing alopecia areata, vitiligo.Research shows that its extract is activation and rise effect to tyrosinase activity and generation melanocyte amount.
Herba Ecliptae: sweet in the mouth, acid, salty, cold in nature.Liver-nourishing kidney-boosting, cooling blood for hemostasis, crow must hair color." detailed outline " " black beard and hair, kidney tonifying is cloudy "; " Bencao Jingshu " " Eclipta prostrata is apt to removing heat from blood.The white person of beard and hair ... then beard and hair blackening, and tooth is also therefore and admittedly.So the grass that bleaches at all times, when being victory now." ancient book notebook article dry and grind the end, put root of hair in the eyebrow place on the skin, can make the blackening that thickens of sparse eyebrow, the poliosis blackening.
Fructus Ligustri Lucidi: property is sweet, bitter, flat, the function tonification Liver and kidney, and black hair makes eye bright.Compendium of Material Medica: " reinforcing YIN-essence, strong waist knee joint bleach and send out, and make eye bright ".Can breed hair follicle cell, promote hair growth, strengthen tyrosinase activity, increase melanic generation, be used to treat early whitening of beard and hair.
Semen Cuscutae: property is sweet, temperature, function tonification Liver and kidney, blood nourishing and hair.Modern study shows that its extract has the Mushroom Tyrosinase of rise and melanocyte growing amount, impels the effect of hair blackening.
Spica Prunellae: bitter, hot, cold.The function heat-clearing and toxic substances removing, mass dissipating and swelling eliminating.Research shows, but its extract activity of tyrosinase.
Herba Lycopi: hardship, suffering, tepor.The function promoting blood circulation to restore menstrual flow, treatment early whitening of hair and hair are not given birth to.Research shows that its extract has the effect that activates tryrosinase.
Herba Menthae: nature and flavor are hot, and cold has dispelling wind-heat, refresh oneself, the effect of rash.Withered to head pruritus, hair, oils and fats too much, seborrheic alopecia and tinea capitis etc. have the improvement effect.Modern study shows that Folium Menthae extract has the effect that strengthens tyrosinase activity.
Rhizoma Chuanxiong: suffering, temperature.Have effects such as blood-activating and qi-promoting, wind-expelling pain-stopping.Its extract can expanded head blood capillary, and blood circulation promoting increases hair nutrition, can make hair that good pliability is arranged and is difficult for the function that becomes fragile, and also can improve the tensile strength and the extensibility of hair, and can delay the poliosis growth.
Rhizoma Zingiberis: acrid in the mouth, hot in nature.External chafe Marjoram Extract improves local blood fortune, can the Chinese medicine nutritional labeling be caused scalp, helps better to absorb.
The specific embodiment
Below in conjunction with specific embodiment the present invention is described further, but not as to qualification of the present invention.
Embodiment 1
Take by weighing raw material (g) according to following weight:
Radix Polygoni Multiflori 20g, Semen Sesami Nigrum 20g, Rhizoma Drynariae 15g, Herba Ecliptae 15g, Fructus Ligustri Lucidi 15g, Semen Cuscutae 15g, Spica Prunellae 15g, Herba Lycopi's 15 weight, Herba Menthae 10g, Rhizoma Chuanxiong 10g, Rhizoma Zingiberis 10g;
The method for preparing of the lotion of the Radix Polygoni Multiflori of the present invention hair color side:
Get Radix Polygoni Multiflori 20g, Semen Sesami Nigrum 20g, Rhizoma Drynariae 15g, Herba Ecliptae 15g, Fructus Ligustri Lucidi 15g, Semen Cuscutae 15g, Spica Prunellae 15g, Herba Lycopi's 15 weight, Herba Menthae 10g, Rhizoma Chuanxiong 10g and Rhizoma Zingiberis 10g and mix, after adding the distilled water merceration 30min of 6 times of amounts of mixed medicinal materials gross weight in the mixed medicinal materials, heating; Boil the back and keep boiling 1h, filter, give the distilled water that adds 4 times of amounts of mixed medicinal materials gross weight in the medicinal residues; Boil the back and keep boiling 30min, filter, merge filtrating twice; Leave standstill 48h; Get supernatant,, promptly get lotion with citric acid adjust pH 6.0-6.5.
Method for using: after user is cleaned hair, evenly water with well-done medicine soup hair is all soaked, the massaging scalp while watering, after 15 minutes, the reuse towel wraps up, and is clean with clear water after 3 hours.
Embodiment 2
Take by weighing raw material (g) according to following weight:
Radix Polygoni Multiflori 35g, Semen Sesami Nigrum 10g, Rhizoma Drynariae 30g, Herba Ecliptae 10g, Fructus Ligustri Lucidi 20g, Semen Cuscutae 5g, Spica Prunellae 20g, Herba Lycopi's 5 weight, Herba Menthae 20g, Rhizoma Chuanxiong 5g, Rhizoma Zingiberis 20g;
The method for preparing of the lotion of the Radix Polygoni Multiflori of the present invention hair color side:
Get Radix Polygoni Multiflori 35g, Semen Sesami Nigrum 10g, Rhizoma Drynariae 30g, Herba Ecliptae 10g, Fructus Ligustri Lucidi 20g, Semen Cuscutae 5g, Spica Prunellae 20g, Herba Lycopi's 5 weight, Herba Menthae 20g, Rhizoma Chuanxiong 5g and Rhizoma Zingiberis 20g and mix, after adding the distilled water merceration 20min of 5 times of amounts of mixed medicinal materials gross weight in the mixed medicinal materials, heating; Boil the back and keep boiling 40min, filter, give the distilled water that adds 3 times of amounts of mixed medicinal materials gross weight in the medicinal residues; Boil the back and keep boiling 20min, filter, merge filtrating twice; Leave standstill 36h; Get supernatant,, promptly get lotion with citric acid adjust pH 6.0-6.5.
Method for using such as embodiment 1.
Embodiment 3
Take by weighing raw material (g) according to following weight:
Radix Polygoni Multiflori 1g, Semen Sesami Nigrum 40g, Rhizoma Drynariae 1g, Herba Ecliptae 40g, Fructus Ligustri Lucidi 30g, Semen Cuscutae 1g, Spica Prunellae 1g, Herba Lycopi's 30 weight, Herba Menthae 30g, Rhizoma Chuanxiong 1g, Rhizoma Zingiberis 1g;
The method for preparing of the lotion of the Radix Polygoni Multiflori of the present invention hair color side:
Get Radix Polygoni Multiflori 1g, Semen Sesami Nigrum 40g, Rhizoma Drynariae 1g, Herba Ecliptae 40g, Fructus Ligustri Lucidi 30g, Semen Cuscutae 1g, Spica Prunellae 1g, Herba Lycopi's 30 weight, Herba Menthae 30g, Rhizoma Chuanxiong 1g and Rhizoma Zingiberis 1g and mix, after adding the distilled water merceration 40min of 7 times of amounts of mixed medicinal materials gross weight in the mixed medicinal materials, heating; Boil the back and keep boiling 80min, filter, give the distilled water that adds 5 times of amounts of mixed medicinal materials gross weight in the medicinal residues; Boil the back and keep boiling 40min, filter, merge filtrating twice; Leave standstill 60h; Get supernatant,, promptly get lotion with citric acid adjust pH 6.0-6.5.
Method for using such as embodiment 1.
Embodiment 4
Take by weighing raw material (g) according to following weight:
Radix Polygoni Multiflori 10g, Semen Sesami Nigrum 35g, Rhizoma Drynariae 10g, Herba Ecliptae 30g, Fructus Ligustri Lucidi 5g, Semen Cuscutae 20g, Spica Prunellae 5g, Herba Lycopi's 20 weight, Herba Menthae 5g, Rhizoma Chuanxiong 20g, Rhizoma Zingiberis 5g;
The method for preparing of the liniment/tincture of the Radix Polygoni Multiflori of the present invention hair color side:
Get Radix Polygoni Multiflori 10g, Semen Sesami Nigrum 35g, Rhizoma Drynariae 10g, Herba Ecliptae 30g, Fructus Ligustri Lucidi 5g, Semen Cuscutae 20g, Spica Prunellae 5g, Herba Lycopi's 20 weight, Herba Menthae 5g, Rhizoma Chuanxiong 20g and Rhizoma Zingiberis 5g and mix, give to add 6 times of amounts of medical material gross weight, 75% ethanol, airtight immersion 30min under the room temperature in the mixed medicinal materials; 85 ℃ of heating and refluxing extraction twice after boiling for the first time, keep boiling 40min; After 4 times of amounts of medicinal residues reuse mixed medicinal materials gross weight, 75% ethanol boils after filtering, keep boiling 30min, filter; Merge filtrating twice, leave standstill 48h, get supernatant; With citric acid adjust pH 6.0-6.5, promptly get liniment/tincture.
Method for using: medicinal liquid is squirted hair, rub while spraying every day 1-2 time, each position 3-5 of reuse finger massage head minute, with comb combing hair repeatedly, make the telangiectasis under the scalp thereafter, blood flow increasing helps medicinal liquid infiltration root of hair.
Embodiment 5
Take by weighing raw material (g) according to following weight:
Radix Polygoni Multiflori 30g, Semen Sesami Nigrum 15g, Rhizoma Drynariae 20g, Herba Ecliptae 25g, Fructus Ligustri Lucidi 10g, Semen Cuscutae 10g, Spica Prunellae 15g, Herba Lycopi's 10 weight, Herba Menthae 15g, Rhizoma Chuanxiong 15g, Rhizoma Zingiberis 10g;
The method for preparing of the liniment/tincture of the Radix Polygoni Multiflori of the present invention hair color side:
Get Radix Polygoni Multiflori 30g, Semen Sesami Nigrum 15g, Rhizoma Drynariae 20g, Herba Ecliptae 25g, Fructus Ligustri Lucidi 10g, Semen Cuscutae 10g, Spica Prunellae 15g, Herba Lycopi's 10 weight, Herba Menthae 15g, Rhizoma Chuanxiong 15g and Rhizoma Zingiberis 10g and mix, give to add 5 times of amounts of medical material gross weight, 75% ethanol, airtight immersion 20min under the room temperature in the mixed medicinal materials; 85 ℃ of heating and refluxing extraction twice after boiling for the first time, keep boiling 20min; After 3 times of amounts of medicinal residues reuse mixed medicinal materials gross weight, 75% ethanol boils after filtering, keep boiling 20min, filter; Merge filtrating twice, leave standstill 36h, get supernatant; With citric acid adjust pH 6.0-6.5, promptly get liniment/tincture.
Method for using such as embodiment 4.
Embodiment 6
Take by weighing raw material (g) according to following weight:
Radix Polygoni Multiflori 40g, Semen Sesami Nigrum 5g, Rhizoma Drynariae 40g, Herba Ecliptae 2g, Fructus Ligustri Lucidi 5g, Semen Cuscutae 5g, Spica Prunellae 25g, Herba Lycopi's 5 weight, Herba Menthae 5g, Rhizoma Chuanxiong 25g, Rhizoma Zingiberis 5g;
The method for preparing of the liniment/tincture of the Radix Polygoni Multiflori of the present invention hair color side:
Get Radix Polygoni Multiflori 40g, Semen Sesami Nigrum 5g, Rhizoma Drynariae 40g, Herba Ecliptae 2g, Fructus Ligustri Lucidi 5g, Semen Cuscutae 5g, Spica Prunellae 25g, Herba Lycopi's 5 weight, Herba Menthae 5g, Rhizoma Chuanxiong 25g and Rhizoma Zingiberis 5g and mix, give to add 7 times of amounts of medical material gross weight, 75% ethanol, airtight immersion 40min under the room temperature in the mixed medicinal materials; 85 ℃ of heating and refluxing extraction twice after boiling for the first time, keep boiling 1h; After 5 times of amounts of medicinal residues reuse mixed medicinal materials gross weight, 75% ethanol boils after filtering, keep boiling 40min, filter; Merge filtrating twice; Leave standstill 60h, get supernatant, promptly get liniment/tincture with citric acid adjust pH 6.0-6.5.
Method for using such as embodiment 4.
The present invention has following pharmacological action: Radix Polygoni Multiflori hair color side has the effect of activity of tyrosinase and melanin formation promoting, and above pharmacological action is used to improve poliosis for it is clinical provides experimental basis.
Face the first hair color side water extract (hereinafter to be referred as water extract) of the present invention's preparation and the pharmacodynamic study that ethanol extraction (hereinafter to be referred as ethanol extract) carries out down, its method and result are following:
One, to the influence of tyrosinase activity
1 material and method
1.1 the prescription of Radix Polygoni Multiflori hair color side
Radix Polygoni Multiflori 20g, Semen Sesami Nigrum 20g, Rhizoma Drynariae 15g, Herba Ecliptae 15g, Fructus Ligustri Lucidi 15g, Semen Cuscutae 15g, Spica Prunellae 15g, Herba Lycopi 15g, Herba Menthae 10g, Rhizoma Chuanxiong 10g, Rhizoma Zingiberis 10g.
1.2 the preparation of Radix Polygoni Multiflori hair color formula extraction
1.2.1 the preparation of water extract
Take by weighing above-mentioned 11 flavor medical materials, add the distilled water merceration 30min of 6 times of amounts of medical material total amount after, heating, boil 1h after; Filter, the distilled water that adds 4 times of amounts of medical material gross weight again boils 30min, filters; Merge filtrating twice, boil off solvent and become paste, be dissolved in PH6.8; 0.05mol/L in the phosphate buffer, be diluted to concentration and be 1g/ml, subsequent use.
1.2.2 the preparation of ethanol extract
Take by weighing above-mentioned 11 flavor medical materials, add airtight immersion 30min under 6 times of amounts of medical material gross weight, the 75% ethanol room temperature, 85 ℃ of heating and refluxing extraction twice, boiling time is 40min for the first time; After the filtration, 4 times of amounts of medicinal residues reuse medical material gross weight, 75% ethanol boils 30min, filters; Merge filtrating twice, fling to ethanol and become paste, add the 5mL dimethyl sulfoxine; Reuse pH6.8,0.05mol/L phosphate buffer (PBS) are diluted to concentration and are 1g/ml, and be subsequent use.
1.3 reagent and instrument
Reagent: tryrosinase, levodopa derive from sigma company; Sodium hydrogen phosphate (Tianjin Da Mao chemical reagent factory, lot number: 20090721); (Guangzhou newly becomes the chemical plant to potassium dihydrogen phosphate, lot number: 20010526).
Instrument: ELX800 type ELIASA (U.S. BIOTEK company); SW-CJ-IF type superclean bench (Wujiang is converged and led to cleaning equipment factory); BT224S type electronic analytical balance (Sartorius company); 110S electric-heated thermostatic water bath (SHIPING company).
1.4 the configuration of reagent
1.4.3PBS the configuration of solution
First liquid: 0.05mol/L Na
2HPO
4Solution takes by weighing sodium hydrogen phosphate 7.099g, and adding distil water is to 1000ml.
Second liquid: 0.05mol/L KH
2PO
4Solution takes by weighing potassium dihydrogen phosphate 6.803g, and adding distil water is to 1000ml.
First, second liquid is divided in the brown bottle, in 4 ℃ of refrigerators, preserves, time spent first, second two liquid mix by same ratio, can obtain the PBS liquid of pH=6.8.
1.4.1 the preparation of tryrosinase solution
Accurately take by weighing the 25ku tryrosinase, be dissolved among the 125ml PBS, i.e. enzyme activity unit 200u/ml, jolting is dissolved subsequent use.
1.4.2 the preparation of levodopa solution
The 1h precision takes by weighing levodopa 20mg before the measuring, be dissolved among the 50mlPBS, i.e. and concentration 0.4mg/ml, jolting, fully the dissolving back is subsequent use.
1.4.4 receive the preparation of reagent thing
With concentration be 2 Radix Polygoni Multiflori hair color side concentrated solutions of 1g/ml to use freshly prepared PBS solution dilution respectively be high, medium and low three dose concentrations, be respectively 1mg/ml, 0.5mg/ml, 0.25mg/ml.
2 experimental techniques
The Radix Polygoni Multiflori hair color side diluent 100ul of the variable concentrations of above configuration is mixed with tryrosinase 50ul, behind 36.8 ℃ of thermostat water bath incubation 5min, add 50ul L-dopa and continue incubation 2min, immediately in ELIASA 490nm place's photometry density OD value.Each sample repeated measure 6 times is averaged, and calculates the activity ratio of tryrosinase again.
The tyrosinase activity activity ratio is pressed formula and is calculated: activity ratio %=[(C-D)-(A-B)]/(A-B) * 100
In the formula: A=A475-medicine+enzyme
B=A475-medicine-enzyme
C=A475+ medicine+enzyme
D=A475+ medicine-enzyme
Wherein,
The A value is the OD value of tyrosine solution+50ul 0.4%L-dopa solution of 100ul medicinal liquid+50ul 500u/ml.The B value is the OD value of 100ul medicinal liquid+50ulPBS solution+50ul 0.4%L-dopa solution.
The C value is the OD value of tyrosine solution+50ul 0.4%L-dopa solution of 100ul PBS+50ul 500u/ml.The D value is the OD value of 100ul PBS+50ul PBS solution+50ul 0.4%L-dopa solution.
3 experimental results
Table 1 result shows, Radix Polygoni Multiflori hair color side water extract and ethanol extract except that ethanol extract 0.25mg/ml concentration, all have the effect that activates tryrosinase, and each Chinese prescription is along with the increase of concentration, and tyrosinase activity increases gradually, presents dose-dependence.
Table 1 Radix Polygoni Multiflori hair color side's water extract and ethanol extract are to the influence of tryrosinase
Two, to melanogenic influence
1 material and method
1.1 main agents and instrument
The source of mouse black-in tumor cell B16 cell strain: from Shanghai cell research institute of the Chinese Academy of Sciences.
Reagent: DMEM high glucose medium (U.S. Hyclone company); Hyclone (U.S. Hyclone company); Trypsin U.S. Sigma company); Tetramethyl azo tetrazolium bromide (MTT, Amersco company); Dimethyl sulfoxide (1Guanghua Chemical Plant Co., Ltd., Guangdong).
Instrument: ELX800 type ELIASA (U.S. BIOTEK company); BH2-RFL-T2 type inverted microscope (OLYMPUS company); 311 type CO
2Incubator (Thermo company); SW-CJ-IF type superclean bench (Wujiang is converged and led to cleaning equipment factory); 3K18 type high speed tabletop centrifuge (U.S. Sigma company); BT224S type electronic analytical balance (Sartorius company); 110S type electric-heated thermostatic water bath (SHIPING company).
1.2 the prescription of Radix Polygoni Multiflori hair color side
Radix Polygoni Multiflori 20g, Semen Sesami Nigrum 20g, Rhizoma Drynariae 15g, Herba Ecliptae 15g, Fructus Ligustri Lucidi 15g, Semen Cuscutae 15g, Spica Prunellae 15g, Herba Lycopi 15g, Herba Menthae 10g, Rhizoma Chuanxiong 10g, Rhizoma Zingiberis 10g.
1.3 the preparation of Radix Polygoni Multiflori hair color formula extraction
1.3.1 the preparation of water extract
Take by weighing above-mentioned 11 flavor medical materials, add the distilled water merceration 30min of 6 times of amounts of medical material gross weight after, heating, boil 1h after; Filter, the distilled water that adds 4 times of amounts of medical material gross weight again boils 30min, filters; Merge filtrating twice, boil off solvent and become paste, be dissolved in pH6.8; 0.05mol/L in the phosphate buffer, be diluted to concentration and be 1g/ml, subsequent use.
1.3.2 the preparation of ethanol extract
Take by weighing above-mentioned 11 flavor medical materials, add airtight immersion 30min under 6 times of amounts of medical material gross weight, the 75% ethanol room temperature, twice of 85 ℃ of heating and refluxing extraction; Boiling time is 40min for the first time, filters 4 times of amounts of back medicinal residues reuse medical material gross weight, 75% ethanol and boils 30min, filters; Merge filtrating twice, fling to ethanol and become paste, add the 5mL dimethyl sulfoxine; Reuse pH6.8,0.05mol/L phosphate buffer (PBS) are diluted to concentration and are 1g/ml, and be subsequent use.
2 experimental techniques
2.1 the cultivation of mice B16 melanotic tumor cell
Mouse black-in tumor cell B16 cell strain is when being cultured to nearly fusion state with the high sugared DMEM culture medium (containing the 100U/ml penicillin, the 100g/ml streptomycin) that contains 10%PBS; With 0.25% trypsin and 0.02%EDTA digestion; Centrifugal, with above-mentioned culture medium re-suspended cell, the adjustment cell density is 1 * 10
5/ ml plants 96 orifice plates (being used for cell proliferation test) and 6 orifice plates (being used for melanin content measures) respectively.Behind the cell inoculation 24h, change liquid, measure after continuing to hatch 48h (cell proliferation test) and 72h (melanin content mensuration) with the fresh culture that adds medicine.
2.2 being tried preparation of thing solution and experiment divides into groups
With serum-free DMEM culture medium dilution 100mg/ml, filtration sterilization is 10mg/ml with the coubling dilution dilution more respectively for water extract and alcohol extract; 5mg/ml, 2.5mg/ml, 1.25mg/ml; 0.625mg/ml, 0.3125mg/ml, 0.15625mg/ml; 0.078125mg/ml the blank group is to contain the culture medium (pure water or ethanol) of being tried the thing coordinative solvent.
2.3 the mensuration of melanin content
In 6 orifice plates, (inoculum density is 1 * 10 to every hole 1000l with the B16 cell inoculation of exponential phase
5/ ml).Behind the 24h, inhale and go culture medium, handle fresh culture (5mg/ml, the 2.5mg/ml of factor to contain variable concentrations; 1.25mg/ml 0.625mg/ml 0.3125mg/ml) changes liquid, every hole 2000l; If not dosing blank after continuing to cultivate 72h, is inhaled and gone culture medium, wash with PBS; Trypsinization stops digestion, collects Digestive system.Digestive system changes the 1.5mlEP pipe respectively in each hole, and is centrifugal, removes supernatant, adds 10% trichloroacetic acid, centrifugal, removes supernatant, adds 1NNaOH solution, and 80 ℃ of dissolvings extremely fully of water-bath change ELISA Plate over to, measure every hole 490nm light absorption value with ELIASA immediately.Each experiment repetition 4 times.Melanocyte appreciation rate (A drug group-A blank group)/A blank group * 100%
2.4 statistical method
The data of surveying with all use
the expression; The check of employing one way analysis of variance, statistic processes use the SPSS13.0 statistical software to accomplish.
3 experimental results
Table 2 result shows, with the blank group relatively, Radix Polygoni Multiflori hair color side each concentration of water extract and blank group more all have significant difference, show as obviously the increasing of melanin content (P＜0.05).Each concentration group of ethanol extract all has the effect that melanin content generates of raising, with the blank group relatively, concentration shows as obviously the increasing of melanin content (P＜0.05) when being 2.5mg/ml and 5mg/ml.
Table 2 Radix Polygoni Multiflori hair color side's water extract and ethanol extract are to the synthetic influence of melanoma cells melanocyte (
A value)
Annotate: * representes to compare with blank control group, and there is obvious facilitation P＜0.05.
Three, conclusion
Find through this experimentation: Radix Polygoni Multiflori hair color side water extract and ethanol extract have the effect that activates tryrosinase and melanin formation promoting.
Above-described embodiment, the present invention specific embodiment a kind of more preferably just, common variation that those skilled in the art carries out in technical scheme scope of the present invention and replacement all should be included in protection scope of the present invention.
Claims (6)
1. Radix Polygoni Multiflori hair color Chinese medicine composition, mixed by following medicinal materials in part by weight:
1～40 part of Radix Polygoni Multiflori, 1～40 part of Semen Sesami Nigrum, 1～40 part of Rhizoma Drynariae, 1～40 part of Herba Ecliptae, 1～30 part of Fructus Ligustri Lucidi, 1～30 part of Semen Cuscutae, 1～30 part of Spica Prunellae, 1～30 part of Herba Lycopi, 1～30 part of Herba Menthae, 1～30 part of Rhizoma Chuanxiong, 1～30 part of Rhizoma Zingiberis.
2. Radix Polygoni Multiflori hair color Chinese medicine composition according to claim 1 is characterized in that: mixed by following medicinal materials in part by weight:
10～35 parts of Radix Polygoni Multiflori, 10～35 parts of Semen Sesami Nigrums, 10～30 parts of Rhizoma Drynariae, 10～30 parts of Herba Ecliptaes, 5～20 parts of Fructus Ligustri Lucidi, 5～20 parts of Semen Cuscutae, 5～20 parts of Spica Prunellaes, 5～20 parts of Herba Lycopi, 5～20 parts of Herba Menthaes, 5～20 parts of Rhizoma Chuanxiongs, 5～20 parts of Rhizoma Zingiberiss.
3. Radix Polygoni Multiflori hair color Chinese medicine composition according to claim 1 is characterized in that: mixed by following medicinal materials in part by weight:
20 parts of Radix Polygoni Multiflori, 20 parts of Semen Sesami Nigrums, 15 parts of Rhizoma Drynariae, 15 parts of Herba Ecliptaes, 15 parts of Fructus Ligustri Lucidi, 15 parts of Semen Cuscutae, 15 parts of Spica Prunellaes, 15 parts of Herba Lycopi, 10 parts of Herba Menthaes, 10 parts of Rhizoma Chuanxiongs, 10 parts of Rhizoma Zingiberiss.
4. a Radix Polygoni Multiflori hair color pharmaceutical composition is characterized in that, is made up of each described Chinese medicine composition and pharmaceutical carrier in the claim 1～3 of effective dose.
5. the method for preparing of a Radix Polygoni Multiflori hair color lotion may further comprise the steps:
The method for preparing of lotion: the weighting profit requires each described Radix Polygoni Multiflori hair color Chinese medicine composition of 1-3 to mix, and behind the distilled water merceration 20-40min that adding mixed medicinal materials gross weight 5-7 doubly measures in the mixed medicinal materials, heating is boiled the back and kept the 40min-80min that seethes with excitement; Filter, give to add the distilled water that mixed medicinal materials gross weight 3-5 doubly measures in the medicinal residues, boil the back and keep boiling 20-40min; Filter, merge filtrating twice, leave standstill 36-60h; Get supernatant,, promptly get lotion with citric acid adjust pH 6.0-6.5.
6. the method for preparing of a Radix Polygoni Multiflori hair color liniment/tincture may further comprise the steps:
The method for preparing of liniment/tincture: the weighting profit requires each described Radix Polygoni Multiflori hair color Chinese medicine composition of 1-3 to mix, and doubly measures 75% ethanol, airtight immersion 20-40min under the room temperature to adding medical material gross weight 5-7 in the mixed medicinal materials; 85 ℃ of heating and refluxing extraction twice after boiling for the first time, keep boiling 20-60min; Filtration back medicinal residues reuse mixed medicinal materials gross weight 3-5 doubly measures 75% ethanol and boils, and keeps boiling 20-40min, filters; Merge filtrating twice, leave standstill 36-60h, get supernatant; With citric acid adjust pH 6.0-6.5, promptly get liniment/tincture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110007820XA CN102068401B (en) | 2011-01-14 | 2011-01-14 | Tuber fleeceflower root hair blackening formula and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110007820XA CN102068401B (en) | 2011-01-14 | 2011-01-14 | Tuber fleeceflower root hair blackening formula and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102068401A CN102068401A (en) | 2011-05-25 |
| CN102068401B true CN102068401B (en) | 2012-06-06 |
Family
ID=44027280
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110007820XA Active CN102068401B (en) | 2011-01-14 | 2011-01-14 | Tuber fleeceflower root hair blackening formula and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102068401B (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104146900A (en) * | 2014-07-25 | 2014-11-19 | 安徽省霍山县聚一科技有限公司 | Tea seed cake natural lotion |
| CN104857487A (en) * | 2015-06-01 | 2015-08-26 | 田艺儿 | Traditional Chinese medicine with effect of turning white hair into black hair and preparation method of traditional Chinese medicine |
| CN105311272A (en) * | 2015-10-15 | 2016-02-10 | 赵焰 | Hair gardening hair melanin factor solution and preparation process thereof |
| CN106038400A (en) * | 2016-06-28 | 2016-10-26 | 龙梅奎 | Hair-care shampoo |
| CN107050254A (en) * | 2017-06-07 | 2017-08-18 | 湖南云从科技有限公司 | It is a kind of to treat pharmaceutical formulation of white hair and preparation method thereof |
| CN110075252A (en) * | 2019-05-13 | 2019-08-02 | 钟金珍 | Chinese medicine growing and blacking hair medical fluid, preparation method and application method |
| CN110251440A (en) * | 2019-08-06 | 2019-09-20 | 巩义市育发堂科贸有限公司 | Black dense Essence of one kind and preparation method thereof |
| CN111714623A (en) * | 2020-07-01 | 2020-09-29 | 霸州市巨隆生物科技有限公司 | Traditional Chinese medicine composition for activating tyrosinase activity and preparation method thereof |
| CN114533606A (en) * | 2020-11-26 | 2022-05-27 | 广西瑞津实业股份有限公司 | Herbal hair blackening lotion |
| CN113209264A (en) * | 2021-06-01 | 2021-08-06 | 山东中医药大学 | Traditional Chinese medicine composition for reducing grey hair and promoting growth of black hair and preparation method thereof |
| CN113712887A (en) * | 2021-10-20 | 2021-11-30 | 湖南省开元博物馆 | Traditional Chinese medicine hair blackening shampoo and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1208048C (en) * | 2001-01-05 | 2005-06-29 | 唐山王清任制药有限公司 | Nano Chinese medicine piece and its prepn |
| CN1895291A (en) * | 2006-06-09 | 2007-01-17 | 陈永丽 | Nano-Chinese medicinal biological product and its preparation |
-
2011
- 2011-01-14 CN CN201110007820XA patent/CN102068401B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN102068401A (en) | 2011-05-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102068401B (en) | Tuber fleeceflower root hair blackening formula and preparation method thereof | |
| CN102028759B (en) | Chinese medicine hair-darkening empirical formula and preparation method thereof | |
| CN103169631B (en) | A kind of submissive glossy shampoo and preparation method containing Chinese medicine composition | |
| CN101485831B (en) | Chinese medicinal composition for blackening and generating hair and preparation method thereof | |
| CN105997836A (en) | Ferment liquid shampoo and preparation method thereof | |
| US10098372B2 (en) | Whitening composition and the use thereof | |
| CN102552096A (en) | Hair-blackening and hair-growing liquid shampoo and preparation method thereof | |
| CN102488633B (en) | Compound essential oil capable of protecting and developing hairs and preparation method thereof | |
| CN103721144B (en) | A kind of natural plants hair color liquid and preparation method thereof | |
| CN102038626B (en) | Traditional Chinese medicinal hair-restoring and anti-alopecia shampoo and preparation method thereof | |
| CN105125472B (en) | A kind of nourishing black hair hair cream containing natural extract and preparation method thereof | |
| CN101152529B (en) | Externally used compound traditional Chinese medicine painting preparation for treating alopecia and method for preparing the same | |
| CN104263556A (en) | Chinese-prescription propolis whitening soap | |
| CN110123683A (en) | Compound natural small molecule plant extracts phalacrosis prevention and hair generation prescription and products thereof | |
| CN102274152A (en) | Hair blackening and nourishing solution and preparation method thereof | |
| CN112656930A (en) | Traditional Chinese medicine shampoo for growing and blackening hair and preparation method thereof | |
| CN106265324A (en) | A kind of hair care anti-hair loss composition for dryness seborrheic alopecia and application | |
| CN107213070A (en) | A kind of draft shampoo powder and preparation method thereof | |
| CN105686980B (en) | It is a kind of to nourish restoring ocular essence dew of releiving | |
| CN104147103A (en) | Traditional Chinese medicinal composition for reducing dandruff, sterilizing, relieving itching and promoting hair growth and preparation method thereof | |
| CN101536969B (en) | Salt Chinese herbal medicine paste shampoo | |
| CN103751086B (en) | Hair blacking-conditioninpowder powder and preparation method thereof | |
| CN102961317A (en) | Traditional-Chinese-medicine hair-washing and hair-care agent | |
| CN106389284A (en) | Blacking and nourishing hair mask and preparation method of blacking and nourishing hair mask | |
| CN101069674A (en) | Hair-conditioner composition using natural plant as base material and its preparing method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |