CN102060937A - Extraction and separation method for green tea polysaccharides - Google Patents
Extraction and separation method for green tea polysaccharides Download PDFInfo
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- CN102060937A CN102060937A CN 201110045130 CN201110045130A CN102060937A CN 102060937 A CN102060937 A CN 102060937A CN 201110045130 CN201110045130 CN 201110045130 CN 201110045130 A CN201110045130 A CN 201110045130A CN 102060937 A CN102060937 A CN 102060937A
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- green tea
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Abstract
The invention provides an extraction and separation method for green tea polysaccharides. The method comprises the following steps of: leaching green tea powder for 3 hours under the conditions of the solid-to-liquid ratio of 1:20 and the leaching temperature of 60 DEG C; leaching twice; concentrating an obtained filtrate in vacuum; extracting the concentrated filtrate twice by using ethyl acetate; precipitating an aqueous layer by using 95 percent ethanol; redissolving the precipitate; adding the solution of trichloroacetic acid until the final concentration is 3.5 percent; standing overnight; freezing, centrifuging and discarding the precipitate; and freezing and drying supernate after dialyzing at a low temperature to obtain a crude extract of tea polysaccharides.
Description
Technical field: the extraction and separation method that the invention provides a kind of green tea polysaccharide.
Background technology: tea polysaccharide be from tealeaves, extract with protein bound acidic polysaccharose or acid glycoprotein together, be one of important physiologically active ingredient in the tealeaves.Chinese scholars has been carried out big quantity research to tealeaves polyose mixture, finds that tea polysaccharide has effects such as the body immunity of increasing, hypoglycemic, reducing blood-fat, minimizing atherosclerosis, anticoagulation, anti-oxidant, radioprotective.
The extraction and separation method of tea polysaccharide has water extraction, acidic ethanol method, in order to improve the purity of tea polysaccharide, remove simultaneously by the protein of ethanol sedimentation.The invention provides a kind of extraction and separation method through superheated water lixiviate, removal tea-polyphenol, ethanol sedimentation, the Deproteinated tea polysaccharide of trichoroacetic acid(TCA).
Summary of the invention: the extraction and separation method that the objective of the invention is to provide a kind of green tea polysaccharide.
The present invention is achieved in that and takes by weighing the 10g green tea powder of crushing screening, with solid-liquid ratio 1: 20,60 ℃ of water-baths extractions 3 hours, repeats to extract once again, the filtrate that obtains merges final vacuum and concentrates, with ethyl acetate extraction twice, water layer is with 95% ethanol sedimentation, and throw out redissolves.Adding an amount of trichoroacetic acid(TCA) solution to final concentration is 3.5%, standing over night, and frozen centrifugation discards precipitation, and supernatant liquor low temperature dialysis postlyophilization gets the tea polysaccharide crude extract.
Embodiment: take by weighing 10g and be crushed to 80 purpose green tea powder, under 1: 20,60 ℃ water bath condition of solid-liquid ratio, extracted 3 hours, the centrifugal 20min of 5000rpm, filter residue repeats lixiviate once under the same conditions, and is centrifugal, merges filtrate twice, vacuum concentration, with ethyl acetate extraction twice, the water layer that obtains is with 2.5 times of volume 95% ethanol sedimentations again, and throw out is water-soluble.Adding an amount of 80% trichoroacetic acid(TCA) solution to final concentration is 3.5%, and after the standing over night, 4 ℃ of frozen centrifugation 20min of 10000rpm discard precipitation, 24 hours postlyophilizations of 4 ℃ of low temperature dialysis of supernatant liquor, the tea polysaccharide crude extract.
Claims (4)
1. the extracting method of a green tea polysaccharide, it is characterized in that: take by weighing the 10g green tea powder of crushing screening, water-bath is extracted under the different treatment condition, 5000rpm/min is centrifugal, the filtrate vacuum concentration that obtains, with ethyl acetate extraction twice, the water layer that obtains is with 2.5 times of volume 95% ethanol sedimentations again, and throw out redissolves.
2. the extracting method of a kind of green tea polysaccharide according to claim 1 is characterized in that the optimal conditions that extracts is: solid-liquid ratio 1: 20,60 ℃ of extraction temperatures, extraction time 3 hours, lixiviate number of times 2 times.
3. the method for removing protein of a green tea polysaccharide, it is characterized in that: through under optimal conditions, extracting the solution after ethanol sedimentation redissolves that obtains, add an amount of 80% trichoroacetic acid(TCA) to different final concentrations, after the standing over night, 4 ℃ of frozen centrifugation 20min of 10000rpm, discard precipitation, 24 hours postlyophilizations of supernatant liquor low temperature dialysis.
4. the method for removing protein of a kind of green tea polysaccharide according to claim 3 is characterized in that: trichoroacetic acid(TCA) concentration was at 3.5% o'clock in the solution, and protein content is minimum in the supernatant liquor, and it is best to remove the albumen effect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201110045130 CN102060937A (en) | 2011-02-25 | 2011-02-25 | Extraction and separation method for green tea polysaccharides |
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| CN 201110045130 CN102060937A (en) | 2011-02-25 | 2011-02-25 | Extraction and separation method for green tea polysaccharides |
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| CN102060937A true CN102060937A (en) | 2011-05-18 |
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| CN 201110045130 Pending CN102060937A (en) | 2011-02-25 | 2011-02-25 | Extraction and separation method for green tea polysaccharides |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105367679A (en) * | 2015-12-03 | 2016-03-02 | 镇江市丹徒区南山溪园茶叶专业合作社 | Green tea polysaccharide extraction method |
| CN106478835A (en) * | 2016-11-22 | 2017-03-08 | 广西钱隆投资管理有限公司 | A kind of extracting method of tea polysaccharide |
| CN107417807A (en) * | 2017-08-15 | 2017-12-01 | 浦江县昂宝生物技术有限公司 | A kind of extracting method of algal polysaccharides |
| CN107573429A (en) * | 2016-11-21 | 2018-01-12 | 广西大学 | A kind of method that jasmine tea tea polysaccharide is extracted using the precipitation method |
| CN109320629A (en) * | 2018-12-13 | 2019-02-12 | 成都华高生物制品有限公司 | A kind of extracting method of tea polysaccharide |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100602841B1 (en) * | 2005-02-28 | 2006-07-19 | 고려대학교 산학협력단 | Acidic polysaccharide with cell-binding inhibition activity isolated from green tea and its composition for preventing and treating gastrointestinal diseases |
| CN101755953A (en) * | 2008-10-16 | 2010-06-30 | 曹翠珍 | Green tea extract with blood sugar reducing effect and preparation method thereof |
-
2011
- 2011-02-25 CN CN 201110045130 patent/CN102060937A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100602841B1 (en) * | 2005-02-28 | 2006-07-19 | 고려대학교 산학협력단 | Acidic polysaccharide with cell-binding inhibition activity isolated from green tea and its composition for preventing and treating gastrointestinal diseases |
| CN101755953A (en) * | 2008-10-16 | 2010-06-30 | 曹翠珍 | Green tea extract with blood sugar reducing effect and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 《中药材》 20050425 许旋等 正交试验优化绿茶中茶多糖醇析水提法的实验研究 , 第04期 * |
| 《化学工程》 20061025 李振武等 茶叶活性成分综合提取过程研究 , 第10期 * |
| 《天然产物研究与开发》 20061030 王元凤等 不同溶剂分级提取的茶多糖的组成及降血糖活性 , 第05期 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105367679A (en) * | 2015-12-03 | 2016-03-02 | 镇江市丹徒区南山溪园茶叶专业合作社 | Green tea polysaccharide extraction method |
| CN107573429A (en) * | 2016-11-21 | 2018-01-12 | 广西大学 | A kind of method that jasmine tea tea polysaccharide is extracted using the precipitation method |
| CN106478835A (en) * | 2016-11-22 | 2017-03-08 | 广西钱隆投资管理有限公司 | A kind of extracting method of tea polysaccharide |
| CN107417807A (en) * | 2017-08-15 | 2017-12-01 | 浦江县昂宝生物技术有限公司 | A kind of extracting method of algal polysaccharides |
| CN109320629A (en) * | 2018-12-13 | 2019-02-12 | 成都华高生物制品有限公司 | A kind of extracting method of tea polysaccharide |
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Application publication date: 20110518 |