CN102058589A - 13,14-dihydroxyl matrine bactericide and application thereof - Google Patents
13,14-dihydroxyl matrine bactericide and application thereof Download PDFInfo
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- CN102058589A CN102058589A CN2009102373405A CN200910237340A CN102058589A CN 102058589 A CN102058589 A CN 102058589A CN 2009102373405 A CN2009102373405 A CN 2009102373405A CN 200910237340 A CN200910237340 A CN 200910237340A CN 102058589 A CN102058589 A CN 102058589A
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Abstract
The invention belongs to the field of disinfectants, in particular to 13,14-dihydroxyl matrine bactericide and application thereof. The main active ingredient of the 13,14-dihydroxyl matrine bactericide is 13,14-dihydroxyl matrine. The 13,14-dihydroxyl matrine belongs to the derivative of matrine, is a plant-derived bactericide, does not contain any harmful chemical components, has low toxicity to people and livestock, does not have residues and does not pollute the environment. In a sterilizing test, the sterilizing ratio of the bactericide prepared from the 13,14-dihydroxyl matrine on escherichia coli and golden staphylococcus is over 99.9 percent.
Description
Technical field
The invention belongs to the disinfectant field, particularly 13,14-dihydroxy matrine antibacterial and uses thereof.
Technical background
In the antibacterial that uses, chemicals occupies sizable ratio at present, and the life-time service of chemicals has brought problems such as chemicals is residual, environmental pollution to environment.Under the enhanced situation of global environmental consciousness, chemicals is being faced with serious challenge, and plant-derived medicine is just becoming the focus of drug development research.At present, the research and development of plant-derived medicine mainly is divided into four levels: (1) seeks the reactive compound of medicine from native compound, the mixture of the monomer of reactive compound or reactive compound is directly used in is processed into the dissipation medicine; (2) carry out reasonable compatibility with the active constituents of medicine in the different plants (perhaps monomer); (3) the synthetic plant source medicinal compounds of manual simulation; (4) with the plant source active component be the effective ingredient of first guide structure screening of medicaments.The domestic research and development of carrying out at present mainly is in above-mentioned (1) and (2) level, promptly rest in the direct extraction of plant source disinfectant, as the method for preparing vegetable insecticide with Radix Sophorae Flavescentis extract and products thereof (CN 1148937), berberine matrine sterilization and disinsection water preparation (CN 1233235C).Level to the research and development of plant-derived disinfectant is not deep enough, mainly is the active ingredient definite proportion difficulty really of medicine.Different plants, and the effective ingredient difference of the different parts of plant, same kind of plant contains multiple different medicine active ingredient and brings very big difficulty to synthetic, and this also is the synthetic less major reason of plant-derived disinfectant of present manual simulation.How studying on the basis existing, developing novel plant source property antibacterial is the key issue that will solve at present.
13,14-dihydroxy matrine belongs to the derivant of matrine, can adopt semisynthetic method to obtain, and has better water solubility, and bactericidal effect is good.With 13,14-dihydroxy matrine is that the antibacterial document of effective ingredient is not reported as yet.
Summary of the invention
The object of the present invention is to provide a kind of bactericidal effect good, do not have 13 of an environmental pollution, 14-dihydroxy matrine antibacterial.
An also purpose of the present invention is to provide 13, the purposes of 14-dihydroxy matrine antibacterial.
Of the present invention 13,14-dihydroxy matrine antibacterial, main effective ingredient is 13,14-dihydroxy matrine; This 13, the composition and the content of 14-dihydroxy matrine antibacterial are preferably 13 of 2～2.5wt%, the water of 14-dihydroxy matrine and surplus.
Among the present invention 13,14-dihydroxy matrine belongs to the derivant of matrine, 13, the preparation of 14-dihydroxy matrine is to be raw material with the sophocarpine, the employing semisynthesis is synthetic, synthetic method can be with reference to the structural modification and transformation research (Duan Zhenhua, Shaanxi Normal University's Master's thesis: 2007,5) of Radix Sophorae Flavescentis alkaloid.
Of the present invention 13, the preparation method of 14-dihydroxy matrine antibacterial is: get 13,14-dihydroxy matrine is dissolved in and obtains antibacterial in the water, preferably makes 13, and 14-dihydroxy matrine accounts for 2～2.5wt% of antibacterial.
Described 13, the molecular structural formula of 14-dihydroxy matrine is:
Beneficial effect of the present invention and advantage: 13,14-dihydroxy matrine belongs to the derivant of matrine, is plant-derived antibacterial, does not contain any harmful chemical analysis, to the people, animal low toxicity, does not have residual, free from environmental pollution.In bactericidal assay, with 13, the antibacterial that 14-dihydroxy matrine is mixed with all reaches more than 99.9% the sterilizing rate of escherichia coli and golden staphylococci, is respond well antibacterial.
The specific embodiment
Embodiment 1
Take by weighing the 1.23g sophocarpine in flask, add 4ml acetonitrile and 4ml water, keep ice bath, under vigorous stirring, divide in 1 hour to add 0.78g KMnO three times
4, continue reaction 2.5 hours; Behind the stopped reaction, in flask, add 10ml methanol, the Organic substance that obtains after the reaction is dissolved fully, sucking filtration is removed insoluble black solid, regulate filtrate pH value to 10 with 2M NaOH, be 10 filtrate three times with 20ml, 10ml, 5ml chloroform extraction pH value respectively, the combined chloroform phase, and use anhydrous Na
2SO
4Drying is spent the night; After the filtration, concentrated filtrate utilizes normal pressure column chromatography purification concentrated filtrate, and (v/v=1: 1) as eluant, obtain faint yellow solid 0.63g behind the purification, yield is 45%, and faint yellow solid is 13,14-dihydroxy matrine with ethyl acetate/methanol.
Embodiment 2
Get that embodiment 1 obtains 13,14-dihydroxy matrine 2.0g is dissolved in the 98g water, fully shakes up, and obtains 13 of 2wt%, 14-dihydroxy matrine antibacterial.
Embodiment 3
Get that embodiment 1 obtains 13,14-dihydroxy matrine 2.5g is dissolved in the 97.5g water, fully shakes up, and obtains 13 of 2.5wt%, 14-dihydroxy matrine antibacterial.
Embodiment 4
With embodiment 2 obtain 13,14-dihydroxy matrine antibacterial carries out bactericidal assay to staphylococcus aureus and escherichia coli.
Respectively depletion Staphylococcus aureus ATCC 6538, escherichia coli 8099 the 3rd generation～the 14th generation the nutrient agar slant tube fresh cultured thing (incubation time 18 hours～24 hours), drawing 3.0ml～5.0ml diluent (mass concentration is the normal saline solution of 0.1% tryptone) with the 5.0ml suction pipe adds in the slant tube, pressure-vaccum repeatedly, wash the lawn in the slant tube, with the 5.0ml suction pipe bacterium liquid that washes is moved in another sterile test tube subsequently, with electronic mixing 20 seconds, make bacteria suspension.Measure bacteria suspension concentration with the bacterial concentration nephelometry earlier, using diluent (mass concentration is the normal saline solution of 0.1% tryptone) to be diluted to concentration then is 5 * 10
6～5 * 10
7Cfu/ml gets the bacteria suspension 0.5ml after the dilution, adds 0.5ml Organic substance interfering material (3% (W/V) bovine serum albumin) mixing again, then test tube is placed 20 ℃ ± 1 ℃ water-bath 5 minutes.Draw the antibacterial that 4.0ml embodiment 2 obtains, inject respectively the bacteria suspension of staphylococcus aureus ATCC 6538 and escherichia coli 8099 is housed, mixing clocks, wait to act on to stipulated time (positive control is a diluent), get the bacterium medicine mixed liquor that the above-mentioned adding of 0.5ml has antibacterial again, join respectively and 4.5ml nertralizer (mass concentration is that 1% lecithin+mass concentration is the aqueous solution of 1% Tween 80) is housed in test tube, neutralization 10 minutes, draw 1.0ml inoculation plate, do colony counting, calculate killing rate, 13,14-dihydroxy matrine antibacterial is 99.97% to the sterilizing rate of above-mentioned escherichia coli and golden staphylococci.
Embodiment 5
With embodiment 3 obtain 13,14-dihydroxy matrine antibacterial carries out bactericidal assay to staphylococcus aureus and escherichia coli.
Respectively depletion Staphylococcus aureus ATCC 6538, escherichia coli 8099 the 3rd generation～the 14th generation the nutrient agar slant tube fresh cultured thing (incubation time 18 hours～24 hours), drawing 3.0ml～5.0ml diluent (mass concentration is the normal saline solution of 0.1% tryptone) with the 5.0ml suction pipe adds in the slant tube, pressure-vaccum repeatedly, wash the lawn in the slant tube, with the 5.0ml suction pipe bacterium liquid that washes is moved in another sterile test tube subsequently, with electronic mixing 20 seconds, make bacteria suspension.Measure bacteria suspension concentration with the bacterial concentration nephelometry earlier, using diluent (mass concentration is the normal saline solution of 0.1% tryptone) to be diluted to concentration then is 5 * 10
6～5 * 10
7Cfu/ml gets the bacteria suspension 0.5ml after the dilution, adds 0.5ml Organic substance interfering material (3% (W/V) bovine serum albumin) mixing again, then test tube is placed 20 ℃ ± 1 ℃ water-bath 5 minutes.Draw the antibacterial that 4.0ml embodiment 3 obtains, inject respectively the bacteria suspension of staphylococcus aureus ATCC 6538 and escherichia coli 8099 is housed, mixing clocks, wait to act on to stipulated time (positive control is a diluent), get the bacterium medicine mixed liquor that the above-mentioned adding of 0.5ml has antibacterial again, join respectively and 4.5ml nertralizer (mass concentration is that 1% lecithin+mass concentration is the aqueous solution of 1% Tween 80) is housed in test tube, neutralization 10 minutes, draw 1.0ml inoculation plate, do colony counting, calculate killing rate, 13,14-dihydroxy matrine antibacterial is 99.99% to the sterilizing rate of above-mentioned escherichia coli and golden staphylococci.
Claims (3)
1. one kind 13,14-dihydroxy matrine antibacterial is characterized in that: the main effective ingredient of described antibacterial is 13,14-dihydroxy matrine.
2. according to claim 1 13,14-dihydroxy matrine antibacterial is characterized in that: described 13, the composition and the content of 14-dihydroxy matrine antibacterial are 13 of 2～2.5wt%, the water of 14-dihydroxy matrine and surplus.
One kind according to claim 1 and 2 13, the purposes of 14-dihydroxy matrine antibacterial is characterized in that: described 13,14-dihydroxy matrine antibacterial is used for the sterilization to escherichia coli and golden staphylococci.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102373405A CN102058589A (en) | 2009-11-16 | 2009-11-16 | 13,14-dihydroxyl matrine bactericide and application thereof |
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| CN2009102373405A CN102058589A (en) | 2009-11-16 | 2009-11-16 | 13,14-dihydroxyl matrine bactericide and application thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102578111A (en) * | 2012-01-12 | 2012-07-18 | 中国检验检疫科学研究院 | Disinfectant and preparation method thereof |
| CN106518873A (en) * | 2016-11-02 | 2017-03-22 | 南通市肿瘤医院 | Sophocarpidine and oxymatrine derivatives and preparation method and application thereof |
| CN112206250A (en) * | 2020-10-26 | 2021-01-12 | 宁夏职业技术学院（宁夏广播电视大学） | Application of sophora alopecuroide extract in preparation of drugs or bacteriostatic agents for treating drug-resistant diseases of poultry |
| CN115025149A (en) * | 2022-07-28 | 2022-09-09 | 河北科技师范学院 | Chinese herbal medicine composition for promoting growth and preventing diseases of cynoglossus gunther and preparation method thereof |
-
2009
- 2009-11-16 CN CN2009102373405A patent/CN102058589A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102578111A (en) * | 2012-01-12 | 2012-07-18 | 中国检验检疫科学研究院 | Disinfectant and preparation method thereof |
| CN106518873A (en) * | 2016-11-02 | 2017-03-22 | 南通市肿瘤医院 | Sophocarpidine and oxymatrine derivatives and preparation method and application thereof |
| CN112206250A (en) * | 2020-10-26 | 2021-01-12 | 宁夏职业技术学院（宁夏广播电视大学） | Application of sophora alopecuroide extract in preparation of drugs or bacteriostatic agents for treating drug-resistant diseases of poultry |
| CN115025149A (en) * | 2022-07-28 | 2022-09-09 | 河北科技师范学院 | Chinese herbal medicine composition for promoting growth and preventing diseases of cynoglossus gunther and preparation method thereof |
| CN115025149B (en) * | 2022-07-28 | 2023-10-10 | 河北科技师范学院 | Chinese herbal medicine composition for promoting growth and preventing diseases of cynoglossus semilaevis and preparation method thereof |
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Application publication date: 20110518 |