CN102042975A - Research and application of molecular identification-based fluorescence nanocrystal quartz fluorescent sensor for high-selectivity multicomponent saccharide detection - Google Patents
Research and application of molecular identification-based fluorescence nanocrystal quartz fluorescent sensor for high-selectivity multicomponent saccharide detection Download PDFInfo
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Abstract
The invention discloses a fluorescence nanocrystal quartz fluorescent sensor for molecular identification-based multicomponent simultaneous saccharide detection and a method for detecting saccharides by using the same. The invention provides a preparation method of a molecular identification-based fluorescence nanocrystal comb quartz plate, which comprises the following steps: selecting an identifier corresponding to the saccharides; preparing a fluorescence nanocrystal, and carrying out surface modification on the fluorescence nanocrystal according to the literature; and modifying the modified fluorescence nanocrystal onto surfaces of different probes of the comb quartz plate by using layer-by-layer accumulation surface modification technique. The invention also provides a method for multicomponent simultaneous saccharide detection, which comprises the following steps: immersing the modified quartz plate into a food sample solution which is simply pulpified, installing the quartz plate onto a sealed silica dish, and detecting the saccharides in the sample. The invention has the advantages of strong specificity, high sensitivity, short detection time and low cost. The saccharide fluorescence detection method has a quick and simple operation process, and the reaction and the result are automatically completed and recorded by instruments.
Description
Technical field
The present invention relates to on-the-spot high selectivity carbohydrate detection technique field fast, the preparation of the quartzy fluorescent optical sensor of the molecular recognition fluorescence nano of carbohydrate in a kind of test sample of more specifically saying so, the invention still further relates to on-the-spot vegetables, the method for carbohydrate in fruit and the food samples of detecting of the quartzy fluorescent optical sensor of described molecular recognition fluorescence nano that adopt.
Background technology
Sugar be occurring in nature exist quantity at most, distribute the widest and have the organic compound of important biomolecule function.Daily edible sucrose, the starch in the grain, the cellulose in the plant, the glucose in the blood of human body etc. all belong to carbohydrate, and the body from the bacterium to the higher mammal all contains saccharide compound.The abundantest with content in the plant, other biology is a nutriment with carbohydrates such as glucose, starch then, from food, absorb the sugar that changes in the adult, provide energy by metabolism to body, so carbohydrate is as common composition of food. be the main source of body energy.Carbohydrate participates in constituting cell membrane and connective tissue simultaneously. and participate in neural composition, keep normal nervous function, carbohydrate is the important nutrient of needed by human body thus.
Though it is indispensable that carbohydrate is a human body, if excess intake then has following harm:
1. after the excessive carbohydrate that promotes to get fat enters human body. except the normal consumption of blood supply sugar, also can in cell, be converted into fat depot, the people is got fat.Fat then be the key factor of bringing out coronary heart disease, hypertension, diabetes and cancer, unhealthful longevity.
2. when causing the shortcoming of calcium deficiency white sugar to be to become glucose. consume a large amount of Cobastabs and calcium, if excessive sugar and the various sweet foods eaten, then cause the shortage of vitamin B1 and calcium easily.
3. influence children ' s healthy growth, to teen-age grow particularly unfavorable.
4. bring out the carious tooth children and eat sugar too much. eat sugar or various sweet foods before particularly sleeping, can influence oral hygiene, acidic materials easily corrode dentine. and be the major reason of bringing out carious tooth.
5. after the excessive sugar that influences intelligence enters blood. under the effect of insulin, can promote the tryptophane of cranial nerve cell hypersecretion.Tryptophane is the important source material of synthetic hypnosis thrombocytin, and the content increase can make the people feel sleepy, listless, and it is slow to cause information to be transmitted in brain, influences man memory and thinking ability.
6. the normal elastic force of wall of eyeball of affecting one's power of vision is to keep by important substance such as Cobastab and calcium.Bathomorphic generation is relevant with lacking of calcium and chromium.The sugar of Excessive Intake. then easily cause the shortage of Cobastab, calcium and chromium.
7. influence skeleton development sugar and be the not acidic materials of calcic, often eat sugar, sweet food, chocolate or Sugared beverages etc., can consume a large amount of calcium, not only can influence skeleton development, also cause fracture easily.
It is reported that the World Health Organization (WHO) is through the complete investigation to 23 countries. confirm that excessive absorption sugar is the major reason of unhealthful longevity.The mortality curve of population is with the parallel rising of consumption of sugar.Sugar is eaten manyly more, and mortality ratio is high more.Have data to show, the life-span for each person of eating high confectionery thing for a long time will shorten 20 years.
Polysaccharide food is after entering human body simultaneously, and through the hydrolytic action of enzyme, becoming wherein mainly to be glucose by the direct monose that utilizes of human body.Be the blood sugar of indication clinically and enter blood circulation with the glucose that the supply body needs.Blood sugar concentration keeps normal level to have the important physical meaning.Normal person's fasting blood-glucose concentration in the morning is 80-120 milligram %.Fasting blood-glucose concentration surpasses 130 milligrams of % and is called hyperglycaemia.Normal person's blood sugar concentration keeps constant relatively level under neural adjusting.If blood sugar concentration often surpasses normal level, the utilization of sugar gets muddled in the expression body; If blood sugar concentration is low excessively, then serious dysfunction can take place in central nervous system, stupor occurs, i.e. hypoglycemic coma.Hyperglycemic patients can not eat the high food of sugar content, and the high food of the edible sugar content of hypoglycemia person is alleviated the hypoglycemia symptom.Therefore, the detection of carbohydrate has very important significance in the food.
Set up a kind of high sensitivity and the specific quick on-the-spot method that detects carbohydrate, just become current this research field and need one of problem of solution badly.Present existing carbohydrate detection method mainly comprises enzyme process and non-enzyme process etc., and is not enough but these detection methods exist:
1. enzymatic analysis is the selectivity that improves analysis by the selectivity of enzyme, but the maintenance of the immobilization of enzyme and enzymatic activity has limited the application of enzyme process;
2. non-enzymatic analysis mainly utilizes the reductibility of aldehyde radical in the carbohydrate molecule with some material reduction, measure according to variation of its absorbance etc., because these methods are also measured the non-sugared reducing substances of the sub-fraction in the blood (as paddy Guang acyl peptide etc.) together, make its measured value more higher than real concentration.
3. above several method is for the detection and the analysis of carbohydrate, generally all exist detect cost height, testing process complexity, result inaccurate, be unsuitable for shortcoming such as field quick detection, therefore can not satisfy the needs of actual detected.
Summary of the invention
The technical problem to be solved in the present invention has provided that a kind of sample preparation is simple, detection speed is fast, highly sensitive, high specificity, selectivity is strong, no reagent demand, the preparation of the quartzy fluorescent optical sensor of molecular recognition fluorescence nano of detection carbohydrate and the method for detection carbohydrate.
In order to solve the problems of the technologies described above, the present invention realizes by following measure: a kind of quartzy production of fluorescent sensor of molecular recognition fluorescence nano that detects carbohydrate is characterized in that may further comprise the steps:
(1) fluorescence nano of different-grain diameter of preparation high quantum production rate, and it is carried out purification process, be mixed with monodisperse liquor;
(2) select can with the interactional identification body of carbohydrate;
(3) exist lower surface to modify fluorescence nano at catalyzer;
(4) design and produce the piezoid of comb multiprobe structure and the quartzy fluorescence excitation ware of sealing, the saturated fluorescence reagent of shortwave emission is housed in the ware;
(5) utilize self assembly surface modification technology layer by layer etc., the fluorescence nano after the modification is modified on the piezoid surface, make the quartzy fluorescence receptor of many probe molecular recognition fluorescence nanos;
When (6) detecting, place the sealing quartz to excite ware emission light path the comb shape piezoid that is modified with multilayer modification fluorescence nano epitaxial, make the quartzy fluorescence receptor of described multiprobe molecular recognition fluorescence nano;
(7) with the piezoid after the watery hydrochloric acid washing detection, the carbohydrate that adsorbs is washed,, realize recycling to carry out mensuration next time.
The fluorescence nano of modification of the present invention is modified the piezoid surface and be may further comprise the steps:
(1) with used piezoid 0.1mol/L H
2O
2/ H
28O
4Solution cleans, and steams water with secondary then and thoroughly cleans for several times, dries up;
(2) will clean also dry piezoid and handle 10min with the APTS/ toluene solution, and clean with toluene, and dry up, and put into Cd/TGA solution-treated 5min, and use secondary water to clean one time, argon gas dries up.
(3) with the modification fluorescence nano solution sonicated 10-30min for preparing, the fluorescence nano solution that obtains disperseing;
(4) piezoid of handling well in the step (2) is immersed in the fluorescence nano solution that step (3) was handled;
(5) the modification piezoid with preparation in the step (4) thoroughly cleans for several times with secondary steaming water, dries up;
(6) repeating step (2) makes the piezoid that described fluorescence nano is modified to step (5) process 3-6 time.
The present invention is further comprising the steps of:
Use after the comb shape piezoid that is modified with fluorescence nano placed 24h.
Of the present invention is CdTe fluorescence nano solution, CdSe fluorescence nano solution.
Fluorescent reagent of the present invention be 7-amino-4-methylcoumarin, Hydroxycoumarin, grain blue homologue, 4,4 '-diisothiocyanic acid base-2,2 '-talan disodium sulfonate, fluorescer 134, methyl green pyronine styrene or pyrene.
Catalyzer of the present invention is 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride and N-maloyl imines.
Carbohydrate of the present invention identification body is p-aminophenyl boric acid, 3-aminobenzene boric acid, 3-cyanophenylboronic acid, 2-fluorobenzoic boric acid, 4-ethoxybenzene boric acid, 3-nitrobenzene boric acid, 3-methylphenylboronic acid, to ethylbenzene boric acid.
A kind of on-the-spot high selectivity detects the method for carbohydrate simultaneously, it is characterized in that comprising the steps: that comb shape piezoid that the modification fluorescence nano that will make by above-mentioned any one method is modified places the emission light path of the quartzy fluorescence excitation ware of sealing, and cooperate portable fluorospectrophotometer, the carbohydrate in vegetables, fruit and the food samples of simple pulp is detected.
Beneficial effect of the present invention:
1. the quartzy production of fluorescent sensor of molecular recognition carbohydrate fluorescence nano, utilized the high selectivity of fluorescence nano, the fluorescence nano material is connected to the quartzy fluorescent optical sensor of piezoid surface preparation fluorescence nano, makes the quartzy fluorescent optical sensor of prepared carbohydrate fluorescence nano have higher sensitivity, selectivity and sensing range.
2. comb shape piezoid of the present invention is the piezoid with multiprobe structure, can detect various saccharides simultaneously.Utilize the color diversity of quantum dot multiple FAs can be distinguished simultaneously.Comb shape quartz after the modification can directly be immersed in the solid sample of fluid sample or simple pulp and discern, and need not any other special reagent, makes that to detect step simpler, is easier to layman's operation.
With the surface layer by layer modification technique be applied in the middle of the preparation of the quartzy fluorescent optical sensor of fluorescence nano, make the preparation of the quartzy fluorescent optical sensor of molecular recognition carbohydrate fluorescence nano of fluorescence nano synergy have controllability, improved sensitivity, selectivity and the accuracy of quartzy fluorescent optical sensor.
4. in the used quartzy fluorescence excitation ware of the present invention specific saturated fluorescence reagent is housed, utilize the broadband excitation characteristic of fluorescence nano, excite ware can excite all fluorescence nanos simultaneously, and light source is a uniform surface light source, has further improved the accuracy and the sensitivity of quartzy fluorescent optical sensor.
5. the resulting fluorescence nano of the present invention is connected to the quartzy fluorescent optical sensor of carbohydrate fluorescence nano on the piezoid, on fluorescence nano, insert simultaneously the interactional identification body of target molecule, can realize high specific, high sensitivity, the polycomponent fast detecting of carbohydrate in the sample.
6. the high specificity of the quartzy fluorescent optical sensor of molecular recognition fluorescence nano of the present invention, other non-specific molecules does not have influence to testing result in the sample; Highly sensitive, can reach the ng level; Detection speed is fast, finishes the time that a basic testing process only needs 2-3 minute, can realize the high flux screening of great amount of samples at short notice, need not any reagent, and cost is low, detects 1 sample and only need share money, and can realize recycling.
7. the quartzy fluorescent optical sensor of fluorescence nano detects the carbohydrate method, and operation is simple fast, and reaction and result finish and record automatically by instrument, have avoided the influence of subjective factor, and guarantees that good repeatability is arranged, and is convenient to on-the-spot the detection.
Description of drawings
Below in conjunction with the drawings and specific embodiments the present invention is done and to describe in further detail.
Accompanying drawing is comb shape piezoid finishing process and detects principle schematic.
Embodiment
Embodiment 1 (monose is as glucose and galactose)
A kind of detection glucose and the quartzy production of fluorescent sensor of lactose molecule identification fluorescence nano may further comprise the steps:
(1) two kind of different-grain diameter CdTe fluorescence nano formulations prepared from solutions: at N
2Protection is dissolved in NaBH with the Te powder down
4Middle preparation Te presoma is then with CdCl
22.5H
2O and the reaction of TGA reactant liquor are regulated pH, the CdTe quantum dot solution of water-soluble two kinds of different-grain diameters of system;
(2) select can with the identification body p-aminophenyl boric acid of glucose effect; Selection can with the identification body 3-aminobenzene boric acid of galactose effect;
(3) get the CdTe fluorescence nano solution 20 μ L of two kinds of different-grain diameters respectively, ultrasonic dispersion 20min.Glucose identification body p-aminophenyl boric acid, catalyzer 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride PBS buffer solution and N-maloyl imines PBS buffer solution, the CdTe fluorescence nano point solution of corresponding particle diameter, stirred three hours, and obtained the fluorescence nano of finishing; Galactose identification body 3-aminobenzene boric acid, catalyzer 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride PBS buffer solution and N-maloyl imines PBS buffer solution, the CdTe fluorescence nano point solution of corresponding particle diameter, stirred three hours, and obtained the fluorescence nano of finishing;
(4) as shown in the figure, design and produce two probe comb shape piezoids.The modification fluorescence nano that adopts the method for modifying layer by layer to prepare is as stated above modified respectively to the detecting probe surface of two probe comb shape piezoids, the modification fluorescence nano of a kind of FAs of each root probe modification forms the comb shape piezoid that is modified with identification glucose and galactose fluorescence nano;
(5) as shown in the figure, the comb shape piezoid that is modified with glucose and galactose fluorescence nano soaked 2min in the solid sample of fluid sample or simple pulp after, place quartzy fluorescence excitation ware emission light path, constitute molecular recognition and detect glucose and the quartzy fluorescent optical sensor of galactose fluorescence nano simultaneously.To make the quartzy fluorescent optical sensor of molecular recognition glucose and galactose fluorescence nano and cooperate portable luminoscope, glucose in the food samples solution and galactose will be detected;
(6) after the detection, the comb shape piezoid with watery hydrochloric acid washing molecular recognition glucose and galactose fluorescence nano washes away glucose and the galactose that adsorbs, and measures to carry out next time, realizes reusing.
Embodiment 2 (disaccharides, as maltose, sucrose and lactose)
A kind of detection maltose, sucrose and the quartzy production of fluorescent sensor of lactose molecule identification fluorescence nano may further comprise the steps:
(1) three kind of different-grain diameter CdSe quantum dot solution preparation: at N
2Protection is dissolved in preparation Se presoma among the TOP with the Se powder down, the S powder is dissolved in preparation S presoma among the TOP, then with the Cd that makes (OA)
2, reaction, under the condition of oleic acid as stabilizing agent, the CdSe quantum dot solution of three kinds of different-grain diameters of system;
(2) select can with the identification body 3-cyanophenylboronic acid of maltose effect; Selection can with the identification body 2-fluorobenzoic boric acid of sucrose effect; Selection can with the identification body 4-ethoxybenzene boric acid of lactose effect;
(3) get the CdSe fluorescence nano solution 20 μ L of two kinds of different-grain diameters respectively, ultrasonic dispersion 20min.Maltose identification body is to the 3-cyanophenylboronic acid, catalyzer 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride PBS buffer solution and N-maloyl imines PBS buffer solution, the CdSe fluorescence nano point solution of corresponding particle diameter, stirred three hours, and obtained the fluorescence nano of finishing; Sucrose identification body 2-fluorobenzoic boric acid, catalyzer 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride PBS buffer solution and N-maloyl imines PBS buffer solution, the CdSe fluorescence nano point solution of corresponding particle diameter stirred three hours, obtained the fluorescence nano of finishing; Lactose identification body 4-ethoxybenzene boric acid, catalyzer 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride PBS buffer solution and N-maloyl imines PBS buffer solution, the CdSe fluorescence nano point solution of corresponding particle diameter, stirred three hours, and obtained the fluorescence nano of finishing;
(4) as shown in the figure, design and produce three probe comb shape piezoids.The modification fluorescence nano that adopts the method for modifying layer by layer to prepare is as stated above modified respectively to the detecting probe surface of three probe comb shape piezoids, the modification fluorescence nano of a kind of FAs of each root probe modification forms the comb shape piezoid that is modified with identification maltose, sucrose and lactose fluorescence nano;
(5) as shown in the figure, the comb shape piezoid that is modified with maltose, sucrose and lactose fluorescence nano soaked 2min in the solid sample of fluid sample or simple pulp after, place quartzy fluorescence excitation ware emission light path, constitute molecular recognition and detect the quartzy fluorescent optical sensor of maltose, sucrose and lactose fluorescence nano simultaneously, to make the quartzy fluorescent optical sensor of molecular recognition maltose, sucrose and lactose fluorescence nano and cooperate portable luminoscope, the maltose in the food samples solution, sucrose and lactose will be detected;
(6) after the detection, the comb shape piezoid with watery hydrochloric acid washing molecular recognition maltose, sucrose and lactose fluorescence nano washes away maltose, sucrose and the lactose that adsorbs, and measures to carry out next time, realizes reusing.
Embodiment 3 (polysaccharide, as cellulose, starch and glycogen)
A kind of detection fibers element, starch and the quartzy production of fluorescent sensor of glycogen molecule identification fluorescence nano may further comprise the steps:
(1) two kind of different-grain diameter CdTe fluorescence nano formulations prepared from solutions: at N
2Protection is dissolved in NaBH with the Te powder down
4Middle preparation Te presoma is then with CdCl
22.5H
2O and the reaction of TGA reactant liquor are regulated pH, the CdTe quantum dot solution of water-soluble two kinds of different-grain diameters of system;
(2) select can with the identification body 3-nitrobenzene boric acid of cellulose effect; Selection can with the identification body 3-methylphenylboronic acid of starch storage crop; Selection can with the identification body of glycogen effect to ethylbenzene boric acid;
(3) get the CdTe fluorescence nano solution 20 μ L of two kinds of different-grain diameters respectively, ultrasonic dispersion 20min.Cellulose identification body is to 3-nitrobenzene boric acid, catalyzer 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride PBS buffer solution and N-maloyl imines PBS buffer solution, the CdTe fluorescence nano point solution of corresponding particle diameter, stirred three hours, and obtained the fluorescence nano of finishing; Starch identification body 3-methylphenylboronic acid, catalyzer 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride PBS buffer solution and N-maloyl imines PBS buffer solution, the CdTe fluorescence nano point solution of corresponding particle diameter stirred three hours, obtained the fluorescence nano of finishing; Glycogen identification body is to ethylbenzene boric acid, catalyzer 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride PBS buffer solution and N-maloyl imines PBS buffer solution, the CdTe fluorescence nano point solution of corresponding particle diameter stirred three hours, obtained the fluorescence nano of finishing.
(4) as shown in the figure, design and produce three probe comb shape piezoids.The modification fluorescence nano that adopts the method for modifying layer by layer to prepare is as stated above modified respectively to the detecting probe surface of three probe comb shape piezoids, the modification fluorescence nano of a kind of FAs of each root probe modification forms the comb shape piezoid that is modified with identification cellulose, starch and glycogen fluorescence nano.
(5) as shown in the figure, the comb shape piezoid that is modified with cellulose, starch and glycogen fluorescence nano soaked 2min in the solid sample of fluid sample or simple pulp after, place quartzy fluorescence excitation ware emission light path, constitute the molecular recognition quartzy fluorescent optical sensor of detection fibers element, starch and glycogen fluorescence nano simultaneously.To make the quartzy fluorescent optical sensor of molecular recognition cellulose, starch and glycogen fluorescence nano and cooperate portable luminoscope, the cellulose in the food samples solution, starch and glycogen will be detected.
(6) after the detection, the comb shape piezoid with watery hydrochloric acid washing molecular recognition cellulose, starch and glycogen fluorescence nano washes away cellulose, starch and the glycogen that adsorbs, and measures to carry out next time, realizes reusing.
Claims (7)
1. quartzy production of fluorescent sensor of the molecular recognition fluorescence nano that detects carbohydrate is characterized in that may further comprise the steps:
(1) fluorescence nano of different-grain diameter of preparation high quantum production rate, and it is carried out purification process, be mixed with monodisperse liquor;
(2) select can with the interactional identification body of carbohydrate;
(3) exist lower surface to modify fluorescence nano at catalyzer;
(4) design and produce the piezoid of comb multiprobe structure and the quartzy fluorescence excitation ware of sealing, the saturated fluorescence reagent of shortwave emission is housed in the ware;
(5) utilize self assembly surface modification technology layer by layer etc., the fluorescence nano after the modification is modified on the piezoid surface, make the quartzy fluorescence receptor of many probe molecular recognition fluorescence nanos;
When (6) detecting, place the sealing quartz to excite ware emission light path the comb shape piezoid that is modified with multilayer modification fluorescence nano epitaxial, make the quartzy fluorescence receptor of described multiprobe molecular recognition fluorescence nano;
(7) with the piezoid after the watery hydrochloric acid washing detection, the carbohydrate that adsorbs is washed,, realize recycling to carry out mensuration next time.
2. carbohydrate of the present invention identification body is p-aminophenyl boric acid, 3-aminobenzene boric acid, 3-cyanophenylboronic acid, 2-fluorobenzoic boric acid, 4-ethoxybenzene boric acid, 3-nitrobenzene boric acid, 3-methylphenylboronic acid, to ethylbenzene boric acid.
3. the preparation method of the quartzy fluorescent optical sensor of multiprobe molecular recognition fluorescence nano according to claim 1, it is characterized in that: described fluorescence nano with modification is modified the piezoid surface and be may further comprise the steps:
(1) with used piezoid 0.1mol/L H
2O
2/ H
2SO
4Solution cleans, and steams water with secondary then and thoroughly cleans for several times, dries up;
(2) will clean also dry piezoid and handle 10min with the APTS/ toluene solution, and clean with toluene, and dry up, and put into Cd/TGA solution-treated 5min, and use secondary water to clean one time, argon gas dries up;
(3) with the modification fluorescence nano solution sonicated 10-30min for preparing, the fluorescence nano solution that obtains disperseing;
(4) piezoid of handling well in the step (2) is immersed in the fluorescence nano solution that step (3) was handled;
(5) the modification piezoid with preparation in the step (4) thoroughly cleans for several times with secondary steaming water, dries up;
(6) repeating step (2) makes the piezoid that described fluorescence nano is modified to step (5) process 3-6 time.
4. the preparation method of multiprobe quantum dot molecular engram quartz transducer according to claim 1 is characterized in that: described comb shape piezoid is a rectangle, and number of probes is 2～4 probes.Described sealing fluorescence excitation ware is the cuboid silica dish, in saturated shortwave emitting fluorescence reagent is housed: 7-amino-4-methylcoumarin, Hydroxycoumarin, grain blue homologue, 4,4 '-diisothiocyanic acid base-2,2 '-talan disodium sulfonate, fluorescence increase from agent 134, methyl green pyronine talan or pyrene.
5. the preparation method of the quartzy fluorescent optical sensor of molecular recognition fluorescence nano according to claim 2, it is characterized in that: described catalyzer is 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride and N-maloyl imines.
6. the quartzy production of fluorescent sensor of multiprobe molecular recognition fluorescence nano according to claim 1 is characterized in that: the piezoid that the multiprobe fluorescence nano is modified is to be installed to and to excite the ware surface by sealing slot that quartz excites the ware surface.
7. an on-the-spot high selectivity detects the method for carbohydrate simultaneously, it is characterized in that comprising the steps: that the multiprobe fluorescence nano quartz transducer with making by claim 1 method is installed on the portable fluorophotometer, the carbohydrate in the solid food sample of liquid food or simple pulp is detected.
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| CN102313725A (en) * | 2011-07-21 | 2012-01-11 | 南开大学 | Preparation method of lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe |
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| CN102854181A (en) * | 2012-09-07 | 2013-01-02 | 南京工业大学 | Nanogold colorimetric sugar measurement method |
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| CN103033495A (en) * | 2012-12-27 | 2013-04-10 | 济南大学 | Research on highly selective multi-component printing molecularly imprinted paper chip fluorescence sensor and on-site detecting application |
| CN103674922A (en) * | 2013-12-23 | 2014-03-26 | 北京科技大学 | Sensor for detecting cancer cells with quantum dot functionalized nanofiber and preparation method |
| CN110257071A (en) * | 2019-05-08 | 2019-09-20 | 华南师范大学 | A kind of quantum dot fluorescence probe and its in detection plant cell glucose application |
| CN110257071B (en) * | 2019-05-08 | 2022-07-05 | 华南师范大学 | Quantum dot fluorescent probe and application thereof in detecting glucose in plant cells |
| CN111413332A (en) * | 2020-04-09 | 2020-07-14 | 吉林大学 | Saccharide distinguishing method based on natural pigment anthocyanin |
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