CN102041000A - Blue-light-excited nickel-chelidamic acid fluorescent film - Google Patents

Abstract

The invention relates to a blue-light-excited nickel-chelidamic acid fluorescent film, introduces the bioinorganic chemical research result of nickel-chelidamic acid complex into the film production technology and discovers that the compound film is luminescent when excited by blue light (460nm). Since the blue light (460nm) is safe for organism, 460nm can be used as exciting light to detect the fluorescent characteristic of a film. Therefore, the compound film can be safely used for biological fluorescence detection.

Description

Blue-light excited nickel-chelidonamic acid fluorescence membrane

Technical field:

The present invention relates to a kind of available blue-light excited luminous nickel-chelidonamic acid film.

Background technology:

2,6-dicarboxyl-4-pyridone (chelidonamic acid), English name 2,6-dicarboxy-4-hydroxypyridine or chelidamic acid are called for short H ₃Chel (following) with being called for short.H ₃Chel is the complex ligand in a typical polygamy site, simultaneously is again the material of biologically active in vivo, and the research of title complex for it all has wide application prospect in coordination chemistry and bio-inorganic chemistry field.U.S. scientist has been found that with H ₃The metal complexes of Chel is as the macromole probe of one of major portion, and DNA isolates effect for organism.The environmentalist also finds, at H ₃Chel and Ni ²⁺After the coordination, reduced Ni ²⁺With contacting of cell surface, thereby alleviated the toxic action of nickel to microorganism.Simultaneously, nickel is again the necessary trace elements of organism (bacterium, plant, animal and human), has been found that at present 4 kinds of bacterial enzymes are to rely on nickel, as urine enzyme (also finding in the plant), CO desaturase (CODH), hydrogen enzyme (H ₂-ASE) and contain the methyl reductase enzyme (MCR) of enzyme prothetic group (F430), the coordination environment of nickel is different in above-mentioned 4 kinds of enzymes, but the nickel center all is positioned at the reactive site of these enzymes.

Summary of the invention:

The present invention has introduced masking technique the bio-inorganic chemistry research of nickel complex first, and has successfully introduced H ₃Chel has found simultaneously that as complex ligand this closes film forming fluorescent characteristic, for nickeliferous metalloprotein of chemical simulation and metalloenzyme, and further investigation H ₃The function of Chel all has great importance.Purpose of the present invention just is to disclose this and closes film forming synthetic and making method.

Technical scheme: use H ₃Chel is as complex ligand and NiCl ₃Reaction obtains blue-light excited nickel-chelidonamic acid fluorescent material Ni (C ₇H ₂NO ₅) (H ₂O) ₃H ₂O0.25MeCN.At Ni (C ₇H ₂NO ₅) (H ₂O) ₃H ₂The interpolation gelifying agent can be made and obtain fluorescence membrane in the homogeneous phase solution of O0.25MeCN.Gelifying agent can be selected H for use ₂NC ₆H ₄CH ₂C ₆H ₄NH ₂

Embodiment:

1, Ni (C ₇H ₂NO ₅) (H ₂O) ₃H ₂O0.25MeCN's is synthetic

Under the condition of 70 ℃ of heating, with the H of 20mL distilled water/3: 1 mixing solutions stirring and dissolving of acetonitrile volume ratio 50mg ₃The Chel powder adds 59mg NiCl ₃6H ₂O, 70 ℃ of heated and stirred 4 hours are cooled to room temperature, obtain Ni (C ₇H ₂NO ₅) (H ₂O) ₃H ₂The homogeneous phase solution of O0.25MeCN.Leave standstill and separate out Ni (C ₇H ₂NO ₅) (H ₂O) ₃H ₂The O0.25MeCN crystal.Reaction formula is as follows:

Ni (C ₇H ₂NO ₅) (H ₂O) ₃H ₂The O0.25MeCN crystal is through infrared and its crystalline structure of X-ray measuring, and its significant parameter is:

2, the making of film

At Ni (C ₇H ₂NO ₅) (H ₂O) ₃H ₂Before the O0.25MeCN green crystal is not separated out, add 50mgH ₂NC ₆H ₄CH ₂C ₆H ₄NH ₂Powder stirred 4 hours as gelifying agent.Under the normal temperature, solvent flashing.When treating that solution becomes the semi-gelled state, get mixing drop 1-2 with glue head dropper and drip on the slide glass of 0.8 * 2.0 (cm).Slide glass is placed on the sol evenning machine, and 100 rev/mins of rotating speeds rotated three seconds.Take off slide glass, vacuum-drying evaporates into film and does typing under the normal temperature, gets the yellow-green colour transparent film.

By above step, success of the present invention synthetic also made nickel-chelidonamic acid film.Simultaneously, we have also carried out ultimate analysis to this film, and have measured its fluorescent characteristic.The results of elemental analyses of this film is: N (%): 7.86; C (%): 45.07; O (%): 4.65.

3, the bioluminescence of safety detects

We can clearly see by fluorometric investigation, and this synthetic film has a fluorescence excitation peak significantly at the 460nm place, a sharp-pointed fluorescence emission peak is arranged at the 502nm place.Because the blue light of 460nm is safe for organism, adopt 460nm to make exciting light, the 502nm emitting fluorescence of detected characteristics, therefore, this compound film can be applied to safe bioluminescence and detect.

Claims (4)

1. blue-light excited nickel-chelidonamic acid fluorescent material, it is characterized by: the chemical formula of this material is Ni (C ₇H ₂NO ₅) (H ₂O) ₃H ₂O0.25MeCN, the crystalline unit cell parameters of this material is a=14.6256

α=90, β=91.397 (4), γ=90 °.

2. fluorescent material as claimed in claim 1, the building-up process that it is characterized by this material is to use chelidonamic acid as part.

3. a fluorescence membrane is characterized by: contain the fluorescent material described in claim 1 or 2 in this film.

4. a fluorescence membrane as claimed in claim 3 is characterized by: this film use H ₂NC ₆H ₄CH ₂C ₆H ₄NH ₂As gelifying agent.