CN102038789A - Quality control method of Chinese herbal preparation-cannabis fruit spleen-enriching pill for treating constipation - Google Patents
Quality control method of Chinese herbal preparation-cannabis fruit spleen-enriching pill for treating constipation Download PDFInfo
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- CN102038789A CN102038789A CN2009100709214A CN200910070921A CN102038789A CN 102038789 A CN102038789 A CN 102038789A CN 2009100709214 A CN2009100709214 A CN 2009100709214A CN 200910070921 A CN200910070921 A CN 200910070921A CN 102038789 A CN102038789 A CN 102038789A
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Abstract
The invention relates to a quality control method of Chinese herbal preparation-cannabis fruit spleen-enriching pill for treating constipation, comprising the following steps of: (1) identifying characters; (2) identifying dried immature fruit of citron orange, angelica and magnolia officinalis by the thin-layer chromatography; (3) identifying white paeony root by the thin-layer chromatography; and (4) determining rheum officinale by the high performance liquid chromatography. The quality control method improves and perfects the quality standard of the Maren Zipi pill, revises the character and the microscopical characters of the Maren Zipi pill on the basis of the original standard, and adds the thin-layer identification detection method of angelica, magnolia officinalis dried immature fruit of citron orange and white paeony root in the prescription; the quality control method also formulates to determine the content of rheum officinale in the prescription by the high performance liquid chromatograph by taking rheumemodin and chrysophanol as reference substances; the revised quality standard improves the controllability of the quality standard of the Maren Zipi pill, further guarantees the inherent quality and the curative effect of the product, and has an important meaning for accelerating product sales, increasing the competitiveness of the product in the market and guaranteeing pharmacy safety for patients.
Description
Technical field
The invention belongs to technical field of Chinese medicines, relate to the detection method of Chinese medicine, especially a kind of method of quality control for the treatment of the Chinese medicine preparation Maren Zipi ball of constipation.
Background technology
The Maren Zipi ball is made up of Radix Et Rhizoma Rhei, Fructus Cannabis, Radix Angelicae Sinensis, Cortex Magnoliae Officinalis, Semen Armeniacae Amarum, Fructus Aurantii Immaturus, Semen Pruni and eight Chinese medicines of the Radix Paeoniae Alba, preparation method is: above eight flavors, Radix Et Rhizoma Rhei, Radix Angelicae Sinensis, Cortex Magnoliae Officinalis, Fructus Aurantii Immaturus, the Radix Paeoniae Alba are ground into fine powder, be ground into fine powder jointly with three flavors such as all the other Fructus Cannabiss again, mixing sieves, and every 100g powder adds refined honey 80～100g, make big honeyed pills, promptly.
Modern clinical empty people and constipation due to intestinal dryness in old people, habitual constipation, the bleeding hemorrhoids controlled commonly used of Maren Zipi ball, constipation due to hemorrhoids belongs to the scorching person of the intestines and stomach, particularly evident in efficacy at the old people's constipation symptom with intestine-dryness due to consumption of fluid, its contained Mel has multiple benefit to human body.For the constipation of old people's dryness of the intestine, except that medicine effect itself, Mel also plays the effect of well moistening.Therefore to capture method of treatment than bitter cold especially suitable for the middle-aged and elderly people constipation for our relaxing the bowels with lubricant drugs method of treatment, so-called " just not hindering down ".
Along with constant development of economy, Chinese medicine is subjected to the more understanding of common people and attractes attention.Chinese medicine is to grow up under traditional instruction of Chinese Medicine theory, and mostly raw material is natural materials such as bark and grass roots, mineral, animal, wide material sources, complicated component.This has just determined the particularity of Chinese medicine safety standards, and the safety standards problem becomes the difficult problem that Chinese medicine enters the international market too.The trend of Chinese medicine standard though the present international standard of still not having plant medicine in the world, the south east asia of the U.S., European Union and China's tradition outlet Chinese medicine are improved.In the case, the standard that more will propose to be fit to China's product quality to be adapting to international standard, and Chinese medicine in state-owned thousands of years use history, countries in the world are also many Chinese medicine standards with reference to China in working out corresponding plant amedica target level of product quality.Quality standard perfect, that improve Chinese medicine has become extremely urgent thing more.
During thin layer chromatography (TLC) is differentiated control medicinal material determine more and more be used in the Chinese medicine preparation, owing to the restriction of reference substance or select reasons such as reference substance is exclusive inadequately, the chromatograph contrast of often selecting for use control medicinal material to differentiate as TLC.
High performance liquid chromatography (HPLC) has fast, sensitive, separation efficiency advantages of higher, in TCD identification, after adopting HPLC method separation characteristic composition, carrying out structural analysis and quantitative analysis with UV-detector (UV) or mass detector (MS), is the effective ways of thoroughly evaluating quality.Just there are 59 kinds of medical materials and 50 kinds of Chinese patent medicines to adopt the HPLC methods to identify in one one of the Chinese Pharmacopoeia (version in 2000), study but also constantly there is human HPLC method that other Chinese medicine is identified with component analysis.
The statement of Maren Zipi ball initial quality standard: " get this product, put microscopically and observe: the peel stone cell is faint yellow to rufous, and polygon, wall thickness are seen in the surface.Stone cell is orange-yellow, shell-like, the thicker broad of wall, on one side pit obvious.Calcium oxalate cluster crystal diameter 18～32 μ m are present in the parenchyma cell, often are arranged in rows, or contain several cluster crystals in a cell.Prism of calcium oxalate is deposited sheet in parenchyma, and oil cell is circular or oval, contains pale brown color grease.Scalariform and reticulate vessel see that diameter is approximately to 80 μ m more.Sometimes visible grease chamber fragment." for the above-mentioned quality control standard of this kind, obviously ten minutes is fuzzy and not enough.The description of part Chinese medicine microscopical identification feature is only arranged in original standard, for quality control standard, be nowhere near, should add control medicinal material and reference substance foundation as the thin layer chromatography identification of test method.The assay item that does not have the index components of the discriminating of feature medical material and reflection medicine inherent quality can't effectively guarantee the inherent quality of product aborning, and drug effect also can not get guaranteeing.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art part, provide a kind of can qualitative and quantitative detection and detection means is simple, the testing result method of quality control of Chinese medicine preparation Maren Zipi ball accurately.
The objective of the invention is to be achieved through the following technical solutions:
A kind of method of quality control for the treatment of the Chinese medicine preparation Maren Zipi ball of constipation, step is:
(1) character identification:
Character description is: the Maren Zipi ball is the big honeyed pills of dark-brown to pitchy; Feeble QI perfume (or spice), bitter in the mouth; Putting microscopically observation Radix Et Rhizoma Rhei is that calcium oxalate cluster crystal is big, diameter 60～140 μ m; That Cortex Magnoliae Officinalis is that stone cell divides is dendritic, wall thickness, laminated striation are obvious; Fructus Aurantii Immaturus is that prism of calcium oxalate is present in the parenchyma in flakes; The Radix Paeoniae Alba is calcium oxalate cluster crystal diameter 18～32 μ m, is present in the parenchyma cell, often is arranged in rows, or contains several cluster crystals in a cell;
(2) thin layer chromatography is differentiated Fructus Aurantii Immaturus, Radix Angelicae Sinensis, Cortex Magnoliae Officinalis:
Adopting thin layer chromatography is reference substance with Fructus Aurantii Immaturus with when being classified as control medicinal material, magnolol, honokiol, differentiates Fructus Aurantii Immaturus, Radix Angelicae Sinensis, Cortex Magnoliae Officinalis in the Maren Zipi ball prescription;
(3) thin layer chromatography is differentiated the Radix Paeoniae Alba:
Adopting thin layer chromatography is reference substance with the peoniflorin, differentiates the Radix Paeoniae Alba in the Maren Zipi ball prescription;
(4) high-efficient liquid phase technique is measured Radix Et Rhizoma Rhei:
Adopting high-efficient liquid phase technique is reference substance with emodin and chrysophanol, measures the Radix Et Rhizoma Rhei in the prescription Maren Zipi ball.
And described thin layer chromatography differentiates that the method for Fructus Aurantii Immaturus, Radix Angelicae Sinensis, Cortex Magnoliae Officinalis is:
1. the Maren Zipi ball is made need testing solution;
2. get the Fructus Aurantii Immaturus control medicinal material and make control medicinal material solution;
3. get magnolol and honokiol reference substance, respectively add methanol and make reference substance solution respectively;
4. get above-mentioned four kinds of solution on lamellae, with the volume ratio cyclohexane extraction: acetone=10: 3 is that developing solvent launches;
5. lamellae is put respectively under ultra-violet lamp 365nm and the 254nm wavelength and inspected, determine whether contain Fructus Aurantii Immaturus, Radix Angelicae Sinensis, Cortex Magnoliae Officinalis composition in the test agent.
And described thin layer chromatography differentiates that the method for the Radix Paeoniae Alba is:
1. the Maren Zipi ball is made need testing solution;
2. get the peoniflorin reference substance, add methanol and make reference substance solution;
3. get need testing solution and reference substance solution on same silica gel thin-layer plate, with the volume ratio chloroform: ethyl acetate: methanol: formic acid=40: 5: 10: 0.2 is that developing solvent launches;
4. analyze the position of the speckle of same color on the silica gel thin-layer plate, determine whether contain Radix Paeoniae Alba composition in the test sample.
And the method that described high-efficient liquid phase technique is measured Radix Et Rhizoma Rhei is:
1. the preparation of standard solution;
2. the preparation of need testing solution;
3. the preparation of negative sample solution;
4. the setting of liquid phase chromatogram condition;
5. measure the Radix Et Rhizoma Rhei chromatogram;
6. interpretation of result: can calculate the content that obtains Radix Et Rhizoma Rhei in the Maren Zipi ball according to chromatogram.
Advantage of the present invention and good effect are:
1, detection method of the present invention is revised character and microscopical identification on the basis of the 15 primary standard of " the Sanitation Ministry medicine standard " Chinese traditional patent formulation preparation, has increased the thin layer identification of test method of control medicinal material and reference substance; And formulated the method that high performance liquid chromatography is carried out assay.Revised quality standard has improved the controllability of drug standard, guarantees the product inherent quality, to promoting production marketing, increase the competitiveness of product in market, guaranteeing that patient's drug safety is significant.
2, the present invention increases the thin layer chromatography diagnostic test project of control medicinal material in the detection method of Maren Zipi ball, the quality control that more helps Chinese medicine preparation, thereby check out counterfeit drug and substandard drug more exactly, go abroad significant for the modernization of Chinese medicine and Chinese medicine.
3, the present invention has carried out raising, perfect to the quality standard of Maren Zipi ball, on the primary standard basis, the character and the microscopical identification feature of Maren Zipi ball have been revised, increased the thin layer identification of test method of Radix Angelicae Sinensis, Cortex Magnoliae Officinalis, Fructus Aurantii Immaturus and the Radix Paeoniae Alba in the prescription, with emodin, chrysophanol is reference substance, has formulated the content that adopts Radix Et Rhizoma Rhei in the high effective liquid chromatography for measuring prescription; Revised quality standard has improved the controllability of Maren Zipi ball quality standard, further guarantees product inherent quality and curative effect, and to promoting production marketing, increase the competitiveness of product in market, guaranteeing that patient's drug safety is significant.
Description of drawings
Fig. 1 is inspect figure 365nm ultra-violet lamp under for Fructus Aurantii Immaturus in the test agent, Radix Angelicae Sinensis, Cortex Magnoliae Officinalis at wavelength for the present invention;
Wherein, band is followed successively by from left to right among Fig. 1: 1. Fructus Aurantii Immaturus negative sample, 2. Fructus Aurantii Immaturus control medicinal material (middle inspection institute), 3. need testing solution 1 (lot number E492001), 4. need testing solution 2 (lot number E492003), 5. need testing solution 3 (lot number E492004), 6. Radix Angelicae Sinensis control medicinal material (middle inspection institute), 7. Radix Angelicae Sinensis negative sample, 8. magnolol, honokiol reference substance (middle inspection institute), 9. Cortex Magnoliae Officinalis negative sample;
Fig. 2 is 254nm ultra-violet lamp under to inspect figure (label implication same Fig. 1) for Fructus Aurantii Immaturus in the test agent, Radix Angelicae Sinensis, Cortex Magnoliae Officinalis at wavelength for the present invention;
Fig. 3 inspects figure for the Radix Paeoniae Alba of the present invention under ultra-violet lamp;
Wherein, the band among Fig. 3 is followed successively by from left to right: negative sample, test sample 1 (lot number: E492001), test sample 2 (lot number: E492003), test sample 3 (lot number: E492004), peoniflorin reference substance (middle inspection institute);
Fig. 4 is the liquid chromatogram of emodin standard substance of the present invention and chrysophanol standard substance;
Fig. 5 is the liquid chromatogram of the present invention for test agent;
Fig. 6 is the liquid form and aspect collection of illustrative plates of Radix Et Rhizoma Rhei negative sample of the present invention.
The specific embodiment
Below in conjunction with embodiment, the present invention is further described, and following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
A kind of method of quality control for the treatment of the Chinese medicine preparation Maren Zipi ball of constipation, its method step is:
(1) character identification:
Character description is: the Maren Zipi ball is the big honeyed pills of dark-brown to pitchy; Feeble QI perfume (or spice), bitter in the mouth.Putting microscopically observation Radix Et Rhizoma Rhei is that calcium oxalate cluster crystal is big, diameter 60～140 μ m; That Cortex Magnoliae Officinalis is that stone cell divides is dendritic, wall thickness, laminated striation are obvious; Fructus Aurantii Immaturus is that prism of calcium oxalate is present in the parenchyma in flakes; The Radix Paeoniae Alba is calcium oxalate cluster crystal diameter 18～32 μ m, is present in the parenchyma cell, often is arranged in rows, or contains several cluster crystals in a cell;
(2) thin layer chromatography is differentiated Fructus Aurantii Immaturus, Radix Angelicae Sinensis, Cortex Magnoliae Officinalis:
Adopt thin layer chromatography with Fructus Aurantii Immaturus with when being classified as control medicinal material, magnolol, honokiol are reference substance, differentiate Fructus Aurantii Immaturus, Radix Angelicae Sinensis, Cortex Magnoliae Officinalis in the Maren Zipi ball prescription.
1. get Maren Zipi ball 5g, shred, add kieselguhr 3g, grind well, add petroleum ether (30～60 ℃) 30ml, supersound process 30 minutes filters, and filtrate volatilizes, and residue adds ethyl acetate 2ml makes dissolving, as need testing solution;
2. get Fructus Aurantii Immaturus control medicinal material 1g, add petroleum ether (30～60 ℃) 20ml, supersound process 10 minutes is shone medical material solution in pairs with legal system; Get Radix Angelicae Sinensis control medicinal material 0.5g again, add petroleum ether (30～60 ℃) 20ml, supersound process 10 minutes is shone medical material solution in pairs with legal system;
3. get magnolol, honokiol reference substance, add methanol and make the mixed solution that every ml contains 1mg, in contrast product solution;
4. according to thin layer chromatography (" an appendix VI of Chinese pharmacopoeia version in 2005 B) test, drawing each 5 μ l of above-mentioned four kinds of solution, put respectively on same silica GF254 lamellae, be developing solvent with cyclohexane extraction-acetone (10: 3), launches, and taking-up is dried;
5. interpretation of result: the lamellae that experiment is obtained is put respectively under ultra-violet lamp 365nm and the 254nm wavelength and is inspected, and (wherein the negative control sample is noiseless separately) the results are shown in Figure 1 (the detection wavelength is 365nm), Fig. 2 (the detection wavelength is 254nm).For in the test agent chromatograph on Fructus Aurantii Immaturus control medicinal material and the corresponding position of Radix Angelicae Sinensis control medicinal material chromatograph, the fluorescence speckle that shows same color, show for test agent and contain Fructus Aurantii Immaturus and Radix Angelicae Sinensis composition, in the test sample chromatograph, with magnolol, the corresponding position of honokiol reference substance chromatograph on, the speckle that shows same color shows for test agent and contains the Cortex Magnoliae Officinalis composition.
(3) thin layer chromatography is differentiated the Radix Paeoniae Alba:
Adopting thin layer chromatography is reference substance with the peoniflorin, differentiates the Radix Paeoniae Alba in the Maren Zipi ball prescription.
1. get Maren Zipi ball 1 ball, shred, add methanol 30ml, supersound process 30 minutes filters, and filtrate evaporate to dryness, residue add methanol 2ml makes dissolving, as need testing solution;
2. get the peoniflorin reference substance in addition, add methanol and make the solution that every ml contains 1mg, in contrast product solution;
3. according to thin layer chromatography (" an appendix VI of Chinese pharmacopoeia version in 2005 B) test, draw need testing solution 10 μ l, reference substance solution 5 μ l, put respectively on same silica gel g thin-layer plate, with chloroform-ethyl acetate-methanol-formic acid (40: 5: 10: 0.2) be developing solvent, launch, take out, dry, spray is with 5% vanillin sulfuric acid solution, and it is clear to be heated to the speckle colour developing;
4. interpretation of result: the testing result of different production batch Maren Zipi ball test samples is seen Fig. 3, in the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color, negative sample is noiseless, illustrates that test sample contains Radix Paeoniae Alba composition;
(4) high-efficient liquid phase technique is measured Radix Et Rhizoma Rhei:
Adopting the Radix Et Rhizoma Rhei in the high-efficient liquid phase technique mensuration prescription Maren Zipi ball, is reference substance with emodin and chrysophanol.
1. the preparation of standard solution: get the emodin reference substance, the chrysophanol reference substance is an amount of, accurately claim surely, add ethyl acetate-dehydrated alcohol (1: 2) mixed solution and make the mixed solution that every 1ml contains emodin 10 μ g, chrysophanol 25 μ g, promptly.
Liquid chromatographic detection the results are shown in Figure 4;
2. the preparation of need testing solution: get the Maren Zipi ball under the weight differential item, shred, get about 2g, the accurate title, decide, and puts in the tool plug conical flask, the accurate methanol 50ml that adds, close plug claims to decide weight, reflux 1 hour, put coldly, claim to decide weight, supply the weight that subtracts mistake with methanol, shake up, filter, precision is measured subsequent filtrate 5ml, put in the flask, heating recovery methanol is to doing, and residue adds hydrochloric acid solution (1 → 10) 10ml, add chloroform 10ml again, put in the water-bath reflux 1 hour, immediately cooling, move in the separatory funnel, divide and get the chloroform layer, the jolting of acid solution reuse chloroform is extracted 3 times, each 15ml, merge chloroform liquid, be recycled to driedly, residue makes dissolving with ethyl acetate-dehydrated alcohol (1: 2) mixed solution, be transferred in the 10ml volumetric bottle, be diluted to scale, shake up, promptly.
Liquid chromatographic detection the results are shown in Figure 5.
3. the preparation of negative sample solution: get the medical material of respectively distinguishing the flavor of except that Radix Et Rhizoma Rhei by prescription, make sample by method for making, press the need testing solution preparation method again, make negative sample solution, by same chromatographic condition analysis, negative sample is not seen chromatographic peak at emodin, the corresponding retention time of chrysophanol place again.The result shows that negative sample is noiseless to detecting.
Liquid chromatographic detection the results are shown in Figure 6.
4. the setting of liquid phase chromatogram condition: chromatographic column is that octadecylsilane chemically bonded silica is a filler, and mobile phase is methanol-0.1% phosphoric acid solution (85: 15), and the detection wavelength is 254nm, and number of theoretical plate calculates by the emodin peak should be not less than 1500.
5. measure: accurate respectively reference substance solution and each 10 μ l of need testing solution of drawing, inject chromatograph of liquid, detect, promptly obtain the chromatogram of various samples.
6. interpretation of result: can determine to contain emodin and chrysophanol by retention time, according to " the every g of content limit of a medical material Radix Et Rhizoma Rhei of Chinese pharmacopoeia version in 2000 contains Radix Et Rhizoma Rhei with emodin (C in the test agent
15H
10O
5), chrysophanol (C
15H
10O
4) the total amount meter, must not be less than 0.50%.Can calculate the content that obtains Radix Et Rhizoma Rhei in the Maren Zipi ball according to chromatogram, this content and state-promulgated pharmacopoeia compare, and whether can judge for the Radix Et Rhizoma Rhei content of test agent qualified, and then judge the quality of the Maren Zipi ball of different manufacturers, different batches.31.37% (the accounting for preparation total amount 15.69%) that Radix Et Rhizoma Rhei accounts for total amount in the Maren Zipi ball in this laboratory sample, in view of the Maren Zipi ball is a starch, require (starch calculates by 80% rate of transform) according to Chinese Pharmacopoeia Commission, every 1g contains Radix Et Rhizoma Rhei with emodin (C
15H
10O
5), chrysophanol (C
15H
10O
4) the total amount meter, must not be less than 0.63mg; Every ball contains Radix Et Rhizoma Rhei with emodin (C
15H
10O
5), chrysophanol (C
15H
10O
4) the total amount meter, must not be less than 5.6mg.
Claims (4)
1. method of quality control for the treatment of the Chinese medicine preparation Maren Zipi ball of constipation, it is characterized in that: the step of method of quality control is:
(1) character identification:
Character description is: the Maren Zipi ball is the big honeyed pills of dark-brown to pitchy; Feeble QI perfume (or spice), bitter in the mouth; Putting microscopically observation Radix Et Rhizoma Rhei is that calcium oxalate cluster crystal is big, diameter 60～140 μ m; That Cortex Magnoliae Officinalis is that stone cell divides is dendritic, wall thickness, laminated striation are obvious; Fructus Aurantii Immaturus is that prism of calcium oxalate is present in the parenchyma in flakes; The Radix Paeoniae Alba is calcium oxalate cluster crystal diameter 18～32 μ m, is present in the parenchyma cell, often is arranged in rows, or contains several cluster crystals in a cell;
(2) thin layer chromatography is differentiated Fructus Aurantii Immaturus, Radix Angelicae Sinensis, Cortex Magnoliae Officinalis:
Adopting thin layer chromatography is reference substance with Fructus Aurantii Immaturus with when being classified as control medicinal material, magnolol, honokiol, differentiates Fructus Aurantii Immaturus, Radix Angelicae Sinensis, Cortex Magnoliae Officinalis in the Maren Zipi ball prescription;
(3) thin layer chromatography is differentiated the Radix Paeoniae Alba:
Adopting thin layer chromatography is reference substance with the peoniflorin, differentiates the Radix Paeoniae Alba in the Maren Zipi ball prescription;
(4) high-efficient liquid phase technique is measured Radix Et Rhizoma Rhei:
Adopting high-efficient liquid phase technique is reference substance with emodin and chrysophanol, measures the Radix Et Rhizoma Rhei in the prescription Maren Zipi ball.
2. the method for quality control of the Chinese medicine preparation Maren Zipi ball of treatment constipation according to claim 1 is characterized in that: described thin layer chromatography differentiates that the method for Fructus Aurantii Immaturus, Radix Angelicae Sinensis, Cortex Magnoliae Officinalis is:
1. the Maren Zipi ball is made need testing solution;
2. get the Fructus Aurantii Immaturus control medicinal material and make control medicinal material solution;
3. get magnolol and honokiol reference substance, respectively add methanol and make reference substance solution respectively;
4. get above-mentioned four kinds of solution on lamellae, with the volume ratio cyclohexane extraction: acetone=10: 3 is that developing solvent launches;
5. lamellae is put respectively under ultra-violet lamp 365nm and the 254nm wavelength and inspected, determine whether contain Fructus Aurantii Immaturus, Radix Angelicae Sinensis, Cortex Magnoliae Officinalis composition in the test agent.
3. the method for quality control of the Chinese medicine preparation Maren Zipi ball of treatment constipation according to claim 1 is characterized in that: described thin layer chromatography differentiates that the method for the Radix Paeoniae Alba is:
1. the Maren Zipi ball is made need testing solution;
2. get the peoniflorin reference substance, add methanol and make reference substance solution;
3. get need testing solution and reference substance solution on same silica gel thin-layer plate, with the volume ratio chloroform: ethyl acetate: methanol: formic acid=40: 5: 10: 0.2 is that developing solvent launches;
4. analyze the position of the speckle of same color on the silica gel thin-layer plate, determine whether contain Radix Paeoniae Alba composition in the test sample.
4. the method for quality control of the Chinese medicine preparation Maren Zipi ball of treatment constipation according to claim 1 is characterized in that: the method that described high-efficient liquid phase technique is measured Radix Et Rhizoma Rhei is:
1. the preparation of standard solution;
2. the preparation of need testing solution;
3. the preparation of negative sample solution;
4. the setting of liquid phase chromatogram condition;
5. measure the Radix Et Rhizoma Rhei chromatogram;
6. interpretation of result: can calculate the content that obtains Radix Et Rhizoma Rhei in the Maren Zipi ball according to chromatogram.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178829A (en) * | 2011-05-06 | 2011-09-14 | 江西九华药业有限公司 | Method for detecting ingredients of Chinese medicinal compound suppository for haemorrhoids |
| CN103110791A (en) * | 2013-02-09 | 2013-05-22 | 项玉荣 | Preparation method of traditional Chinese medicine for treating constipation acute contagious conjunctivitis |
| CN104435679A (en) * | 2014-11-20 | 2015-03-25 | 王正琦 | Traditional Chinese medicine mixture for treating habitual constipation |
| CN104857147A (en) * | 2015-05-28 | 2015-08-26 | 陈秀杰 | Bowel relaxing pill |
-
2009
- 2009-10-22 CN CN2009100709214A patent/CN102038789B/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178829A (en) * | 2011-05-06 | 2011-09-14 | 江西九华药业有限公司 | Method for detecting ingredients of Chinese medicinal compound suppository for haemorrhoids |
| CN103110791A (en) * | 2013-02-09 | 2013-05-22 | 项玉荣 | Preparation method of traditional Chinese medicine for treating constipation acute contagious conjunctivitis |
| CN103110791B (en) * | 2013-02-09 | 2014-04-02 | 项玉荣 | Preparation method of traditional Chinese medicine for treating constipation acute contagious conjunctivitis |
| CN104435679A (en) * | 2014-11-20 | 2015-03-25 | 王正琦 | Traditional Chinese medicine mixture for treating habitual constipation |
| CN104857147A (en) * | 2015-05-28 | 2015-08-26 | 陈秀杰 | Bowel relaxing pill |
| CN104857147B (en) * | 2015-05-28 | 2018-05-01 | 陈秀杰 | One kind relaxes bowel ball |
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| Publication number | Publication date |
|---|---|
| CN102038789B (en) | 2012-02-29 |
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