CN102038076A - Trace element bean-pulp fermented composite peptide chelate compound and preparation method thereof - Google Patents
Trace element bean-pulp fermented composite peptide chelate compound and preparation method thereof Download PDFInfo
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- CN102038076A CN102038076A CN2009102082255A CN200910208225A CN102038076A CN 102038076 A CN102038076 A CN 102038076A CN 2009102082255 A CN2009102082255 A CN 2009102082255A CN 200910208225 A CN200910208225 A CN 200910208225A CN 102038076 A CN102038076 A CN 102038076A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention relates to the fields of novel biological feeds and novel feed additives, in particular to a bean-pulp fermented feed and a fermented bean-pulp amino-acid trace-element chelate compound and preparation methods thereof. In the invention, bean pulp is processed by utilizing a fermentation process, soluble metal salts and peptide or amino acid are proportionally combined by controlling the pH value, the reaction temperature and the reaction time through a covalent bond into the metal-peptide amino-acid chelate compound, and the chelate compound are researched by chemical analysis and an infrared spectrum so as to ensure the formation of the amino-acid chelate compound. The production of the bean-pulp fermented feed and the bean-pulp amino-acid trace-element chelate compound has far-reaching significance, not only can improve the use effect of bean pulp in the feed industry, be favorable to ease the problems of resource waste and environmental pollution caused by superfluously using organic trace element additives, but also favorably solve the problems of high production expenses and high cost of a traditional amino-acid trace-element chelate compound.
Description
(1) technical field
The present invention relates to new bio feed and novel fodder additive field, specifically be meant amino acid trace element chelated thing of bean pulp fermentation feed and fermented bean dregs and production method thereof.The present invention handles dregs of beans by utilizing zymotechnique, make it be rich in microelement chelate, of far-reaching significance, can not only improve the effect that the feedstuff industry dregs of beans uses, also will help to alleviate because the wasting of resources and the problem of environmental pollution that excessive use inorganic microelement additive is caused.Well solved present amino acid trace element chelated thing producing cost height, the problem that cost is high.
(2) background technology
Dregs of beans is a kind of plant protein fodder of excellence.The protein content height, and the amino acid composition is rationally, and nutrition is complete, balanced, is good vegetable protein feed source.Find to contain in the soybean materials such as isoflavones of abundant raising animal immunizing power in recent years again, be subjected to people's welcome especially.But also there are multiple ANFs such as trypsin inhibitor, phytic acid, soybean haemoglutinin, compound sugar, lipoxidase, soybean antigen albumen (sensitizing factor) and goitrin in the dregs of beans.These ANFs can produce harmful effect to health and the production performance of digestion, absorption, metabolism and the animal of nutritional labeling, if a large amount of the use just must make ANFs inactivation, the passivation of soybean.Dregs of beans is the vegetable protein raw material that is most widely used in the feed industry, but the multiple ANFs that wherein exists has reduced absorption and the utilization of animal to the dregs of beans nutriment.Dregs of beans protein content in the dregs of beans is very high, between 43.0～55.0%, and wherein all is water-solubility protein more than 80.0%.Wherein lysine 2.5～3.0%, tryptophan 0.6～0.7%, methionine 0.5～0.7%, cystine 0.5～0.8%, every kilogram of 0.2～0.4mg of carrotene, every kilogram of 3～6mg of thiamine, every kilogram of 3～6mg of riboflavin, every kilogram of 15～30mg of nicotinic acid, every kilogram of 2200～2800mg of choline.Handle dregs of beans with microbe fermentation method and can remove ANFs in the dregs of beans effectively, and the degraded macro-molecular protein generates little peptide, also can generate multiple probio simultaneously, accumulate useful microbial metabolic products, the high-quality protein feed-fermented bean dregs that finally obtains having multiple function.
Amino acid trace element chelated thing is the third generation microelement feed addictive that twentieth century is at first developed by the U.S. seventies.Its collection amino acid and trace element are one, are that trace element has unique chela ring structure with the amino acid hydroxyl through complexation reaction formation-class, and a kind of novel trace mineral supplement of interior absorpting form of analog and biological function form.It is compared with the simple organic salt of the first generation inorganic salts and the second generation, because characteristics such as having that chemical property is stable, biological value is higher, dissolubility is high, easily digest and assimilate, be anti-interference, non-stimulated, many abroad countries are used more widely gradually, are present optimal trace mineral supplements.
The wasting of resources that present abuse trace mineral supplement causes and the environment problem of dying also are that feed is cultured the difficult problem that industry needs to be resolved hurrily.Microelements aminophenol chelated is a nutritional preparation of new generation, and it can replace present widely used inorganic salts to solve the wasting of resources and the problem of environmental pollution that excessive use inorganic additives causes.But because price reasons, its application of culturing on the industry at feed is also very limited.If with the fermented bean dregs is raw material (wherein little peptide and free amino acid can be used as chelating agent), just can reduce the production cost of microelement chelate significantly, make the easier popularization of this good trace mineral supplement of microelement chelate.
So, utilize zymotechnique to handle dregs of beans, make its research work of being rich in microelement chelate of far-reaching significance, can not only improve the effect that the feedstuff industry dregs of beans uses, also will help to alleviate because the wasting of resources and the problem of environmental pollution that excessive use inorganic microelement additive is caused.
Certain research is all being arranged both at home and abroad aspect the biological soybean meal fermenting and producing new product, the report of nearest related developments has: the HP300 of the hamlet company of Denmark and HP400 are that fish meal substitutes protein feed; The POPUP that Taiwan Hui Sheng industrial corporation produces (than manying good fortune/full plain being good for), be biofermentation zero antigen oligosaccharides soya bean protein: the zymoprotein-5000 (MDS-5000) that hamburger, Xi'an biotechnology company produces is high-effect zymoprotein feed; The precious newborn health of Yiwu, Zhejiang China system feed company is the substitute of skimmed milk power and whey powder; The ferment of the U.S. is fed element-Fermsoya, is vegetalitas high protein tunning; The SoycomilK of U.S. ADM company is dregs of beans albumen concentrated feed; Also have Belgium and Holland to wait some companies also doing the research of this respect.Because commercial relation is all maintained secrecy in research method aspect this series products and technology both at home and abroad.The technology property right mostly is offshore company greatly and controls; Even domestic product is also introduced external technology mostly, lack the intellectual property of oneself, so price is also higher relatively, is badly in need of the domestic appearance that the independent intellectual property right product is arranged.But, product in the market is because technical limitations is to occur with single amino acid trace element chelated thing such as chelated copper, chelating zinc, Chelated Manganese, chelated iron, use again after each chelate need being mixed, caused operation loaded down with trivial details, situations such as degree of mixing is inhomogeneous, wastage of material.And this technology at home and abroad yet there are no in the publication, and report is also unexposed to be used.
(3) summary of the invention
One of purpose of the present invention is to provide a kind of bean pulp fermentation feed and micro-bean pulp fermentation composite peptide chelate complex, adopt Bacillus subtillis (40～60%), plant lactobacillus (20～30%), Lactobacillus casei (10～30%), a chelating moulding of trace element bean pulp fermentation composite peptide chelate complex, contain various trace elements, have characteristics such as mixture homogeneity is good, chelating is abundant, rate of ultilization of amino acid is high, cost is low.
Two of purpose of the present invention is to provide a kind of micro-bean pulp fermentation composite peptide chelate complex preparation method easily to go, and is easy and simple to handle, is easy to mass preparation.In order to address the above problem,, explored micro-bean pulp fermentation composite peptide chelate complex and preparation method thereof finally by long-term groping and repetition test research.
The present invention includes two parts content: the production method of fermented bean dregs feed and micro-bean pulp fermentation composite peptide chelate complex production method.
(1) production method of fermented bean dregs feed
The production method of fermented bean dregs feed adopts fermentation mode that dregs of beans is carried out the following technological process of production:
The raw materials for production that fermented bean dregs feed of the present invention is adopted are dregs of beans.
The production method of bean-dregs feed of the present invention mainly comprises the following technological process of production:
1) add the water inoculation: add the water inoculation, amount of water calculates as follows:
Amount of water=material quantity * (41%-adds the preceding white proportion by subtraction of raw material moisture of water)/0.59
Add that the material temperature requirement is 35～40 ℃ behind the water, the bacterial classification that connects accounts for 0.3% of material quantity; The bacterial classification that connects is that aspergillus oryzae is purebred;
2) the ventilation bacterial classification is cultivated: raw material layer thickness is 30cm, and bacterial classification culturing room temperature is 25～38 ℃, and the bacterial classification incubation time is 38～48h, bacterial classification culturing room temperature height, and then the bacterial classification incubation time is short, and bacterial classification culturing room temperature is low, and then the bacterial classification incubation time is long; During the ventilation bacterial classification was cultivated, ventilation was 1000m
3/ m
2, blast is 100pa/m
2
3) granulation oxygen barrier: made grain density is 1.2～1.3g/mm
3In the granulation oxygen barrier, made particle diameter is 4mm, and long is 6mm;
4) heat-preservation fermentation: the fermenting cellar temperature is 25～40 ℃, and fermentation time is 48h; The moisture of the bent material of particle that fermentation becomes is 40%, and temperature is 55～60 ℃;
5) air-dry pulverizing: the moisture content of finished products after air-dry≤12%.In the air-dry pulverizing, the finished product granulometry after the pulverizing is as follows: all by 20 order analyses sieve, the excess on this 20 order analysis sieve is less than 10%;
6) metering packing: select the snakeskin bag airtight package of plastic foil paper bag or band plastic foil for use, weight per package is 15～25kg.
As mentioned above, by the fermentation manufacturing technique after creationary employing is reformed to dregs of beans, make the fermented bean dregs feed, this fermented bean dregs feed is because the ANFs in the removal dregs of beans, multinomial nutritive index is got a promotion, the dregs of beans utilization rate is improved, and the while is also promoted production of fodder efficient, and the production of fodder cost is minimized.
Below just as above in each step technological process of production related technological parameter, relevant device and points for attention be specifically described:
1) adds the water inoculation
1. amount of water: add behind the water raw material water content require be: 41%, amount of water calculates as follows:
Amount of water: material quantity * (the white proportion by subtraction of raw material moisture before the 41%-water)/0.59
2. water temperature is regulated: add that the material temperature requirement is 35～40 ℃ behind the water, can satisfy the material temperature requirement by the method for regulating water temperature
3. bacterial classification: adopt Bacillus subtillis (40～60%), plant lactobacillus (20～30%), Lactobacillus casei (10～30%) fermented by mixed bacterium.
4. inoculum concentration: the bacterial classification consumption accounts for 0.3% of material quantity
5. inoculate mixer: adopt oar blade type stainless steel mixer
2) the ventilation bacterial classification is cultivated
1. thickness of feed layer: 30cm
2. ventilation: 1000m
3/ m
2
3. blast: 100pa/m
2
4. bacterial classification culturing room temperature: 25～38 ℃, optimum temperature is 32 ℃
5. bacterial classification incubation time: 38～48h, bacterial classification culturing room temperature height, then the bacterial classification incubation time is short, and bacterial classification culturing room temperature is low, and then the bacterial classification incubation time is long, can go out song when pending material becomes light green.
3) granulation oxygen barrier
1. granulator: adopt ring die pelleter
2. granular size: diameter 4mm, long 6mm
3. grain density: 1.2～1.3g/mm3
4) heat-preservation fermentation
1. the particle song is expected moisture: 40%, regulate with 65 ℃ of hot water
2. the particle song is expected temperature: 55～60 ℃
3. fermenting cellar temperature: 25～40 ℃
4. fermentation time: 48h, fermentation ends when having treated that dense fermented soya beans, salted or other wise is fragrant
5) air-dry pulverizing
1. moisture content of finished products :≤12%
2. grinding particle size: all by 20 order analyses sieve, the excess on the 20 order analyses sieve is less than 10%
3. dry: that the product of step 4) fermentation finished thoroughly1 is in chronological sequence put into the incubator drying, dried product is added carrier in 1: 0.03～0.04 ratio, pour in the agitator and stir, changing pulverizer then over to pulverizes, Material control is below 45 ℃, material after the pulverizing is crossed 50 eye mesh screens, and sieve back coarse fodder is pulverized again;
4. packing into after sieving has in the bucket or bag of inner bag, tightens pocket, fastens Sign Board, changes warehouse for finished product over to.
6) metering packing
1. select plastics mould airtight package for use
2. weight per package is 25kg
By the as above technological process of production, can obtain the final products of bean-dregs feed of the present invention, the quality standard of this product is as follows:
1) conventional nutritional labeling index
Crude protein 〉=40% crude fat 〉=16.5% crude fibre≤6.0%
Coarse ash≤6.0 lysines 〉=2.4% methionine 〉=6.0%
2) process quality index
Granularity: all by 20 order analyses sieve, the excess on the 20 order analyses sieve is less than 10%
Appearance index: deep yellow to the canescence powder, do not have caking, and fermented soya beans, salted or other wise fragrance is arranged
Product moisture content :≤12%
Sanitary index: should meet the requirement of forage health standard GB13078
Be not difficult to find out that by above quality standard the nutritive index of bean-dregs feed of the present invention truly has lifting, this point can further be known and learn by following measurement data to the raw material dregs of beans:
Table: ferment with dregs of beans part nutritive index (measured value)
| Smell | Fermented soya beans, salted or other wise fragrance |
| Crude protein | 43.7％? |
| Crude fat | 6.6％? |
| Project | Dregs of beans |
| Carbohydrate | 16.2％? |
| VB1? | 0.27mg/100g? |
| VB2? | 0.46mg/100g? |
| Niacin | 6.1mg/100g? |
| B12? | 1.7mg/100g? |
In sum, the present invention is by the fermentation manufacturing technique after creationary employing is reformed to dregs of beans, make bean-dregs feed, this bean-dregs feed is because removed the ANFs in the dregs of beans, multinomial nutritive index is got a promotion, the dregs of beans utilization rate is improved, and the while is also promoted production of fodder efficient, and the production of fodder cost is minimized.
Need to prove that the present invention is by the foregoing description, concrete, full and accurate exposure correlation technique, those skilled in the art can be implemented according to this.And the above embodiment just is used for illustrating the present invention, rather than is used for limiting of the present invention, and interest field of the present invention should be defined by claim of the present invention.Still all should belong to interest field of the present invention as for the change of described technological parameter or the replacement of production equipment etc. herein.
(2) micro-bean pulp fermentation composite peptide chelate complex premix production method
Micro-bean pulp fermentation composite peptide chelate complex of the present invention is mainly made by the raw material of following weight portion:
1. fermented bean dregs
2. 70～80 ℃ of metallopeptide amino-acid chelate molar ratio 2: 1, pH value 6.5, the reaction temperatures and the reaction time 1h that form with covalent bonds in 1: 1～1: 3 (being preferably 1: 2) ratio of soluble metallic salt and peptide or amino acid.And with chemical analysis and infrared spectrum chelate is studied, proved conclusively the formation of amino-acid chelate.
The raw material degree is as follows:
Fermented bean dregs 40～45%
Cupric oxide 5%
Zinc oxide 7%
Manganese carbonate 4%
Ferrous sulfate 7%
KI 0.1%
Sodium selenite 0.01%
Cobalt chloride 0.01%
Water 40～50%
Micro-bean pulp fermentation composite peptide chelate complex product of the present invention has compares down routine characteristics with inorganic microelement:
1. the high concentration lactic acid in the product can significantly improve the digestion and absorption function of animal, and produced simultaneously peptide glycan has increased the immunity of animal to pathogen;
2. the nutritious solid medium of Bacillus subtillis and lactobacillus-fermented when producing polypeptide, produces a large amount of cellulase, hemicellulase, oligopeptide.Small peptide mixture, antibiotic, trace element and other metabolite for cultivated animals provides the best condition of nourishing and growing, can effectively promote growth of animal;
3. adopt dregs of beans as culture medium raw material, reduced production cost, use fermentation and production equipment commonly used simultaneously, be easy in animal-breeding, promote;
4. the microorganism fungus kind that product of the present invention adopts the Ministry of Agriculture's 658 bulletins to allow, because this product adopts chelating technology, animal capable fully absorbs, and does not have hazard residue in the cultivated animals body, does not discharge environmentally safe with ight soil; Having reduced the use amount of antibiotic in breed simultaneously, is a kind of safety.Green microniological proudcts.
5. can effectively reduce the interpolation consumption, product has reduced the premix operation, effectively reduces production cost.
The preparation process that compound amino acid of the present invention is integrated the microelement pre-mix material product is:
1, will pulverize dregs of beans, mixed bacteria liquid (Bacillus subtillis (40～60%), plant lactobacillus (20～30%), Lactobacillus casei (10～30%)) etc. and add fermentation tank in proportion, reaction 48h, 40～55 ℃ of temperature, cooling was emitted after fermentation reaction was finished, and obtained compound peptide and Freamine.
2, add 5% cupric oxide, 7% zinc oxide, 4% manganese carbonate respectively in compound peptide and Freamine, stir, transferring the pH value of solution value with NaOH is to leave standstill 46～54h under 4.4～5.2,20～25 ℃, and filtering-depositing is collected filtrate.
3, the ferrous sulfate (FeSO4) of adding 7% in filtrate is heated to 60～80 ℃, stirring reaction 30～80 minutes.
4, before the spraying according to the purposes of product add 0.1% KI or 0.01% sodium selenite or 0.01% cobalt chloride.
5, be 50～300 ℃ in temperature, spray-drying under the condition of normal pressure fully both must contain the chelating amino acids premix product of various trace elements after the drying.
Dregs of beans of the present invention is big dregs of beans, and soybean or soybean cake are through also realizing the said goods by this law.
Micro-bean pulp fermentation composite peptide chelate complex product preparation method of the present invention compared with prior art has following enthusiasm effect:
1, chelating of multiple different trace element can be shaped by the method for the invention, the chelating degree is even, and chelating is abundant.
2, the composite aminoacid chelating microelement product by this method preparation can directly use as premix, reduces the secondary pollution of production of fodder link.
Claims (1)
1. micro-bean pulp fermentation composite peptide chelate complex and production method thereof
The raw materials for production that 1 fermented bean dregs feed of the present invention is adopted are dregs of beans, and the production method of bean-dregs feed mainly comprises the following technological process of production:
1.1 add the water inoculation: add the water inoculation, amount of water calculates as follows:
Amount of water=material quantity * (41%-adds the preceding white proportion by subtraction of raw material moisture of water)/0.59
Add that the material temperature requirement is 35～40 ℃ behind the water, the bacterial classification that connects accounts for 0.3% of material quantity; The bacterial classification that connects is that aspergillus oryzae is purebred;
1.2 the ventilation bacterial classification is cultivated: raw material layer thickness is 30cm, and bacterial classification culturing room temperature is 25～38 ℃, and the bacterial classification incubation time is 38～48h, bacterial classification culturing room temperature height, and then the bacterial classification incubation time is short, and bacterial classification culturing room temperature is low, and then the bacterial classification incubation time is long; During the ventilation bacterial classification was cultivated, ventilation was 1000m
3/ m
2, blast is 100pa/m
2
1.3 granulation oxygen barrier: made grain density is 1.2～1.3g/mm
3In the granulation oxygen barrier, made particle diameter is 4mm, and long is 6mm;
1.4 heat-preservation fermentation: the fermenting cellar temperature is 25～40 ℃, and fermentation time is 48h; The moisture of the bent material of particle that fermentation becomes is 40%, and temperature is 55～60 ℃;
1.5 air-dry pulverizing: the moisture content of finished products after air-dry≤12%.In the air-dry pulverizing, the finished product granulometry after the pulverizing is as follows: all by 20 order analyses sieve, the excess on this 20 order analysis sieve is less than 10%;
The preparation process of 2 micro-bean pulp fermentation composite peptide chelate complex premix products is:
Add fermentation tank in proportion 2.1 will pulverize dregs of beans, mixed bacteria liquid (Bacillus subtillis (40～60%), plant lactobacillus (20～30%), Lactobacillus casei (10～30%)) etc., reaction 48h, 40～55 ℃ of temperature, cooling was emitted after fermentation reaction was finished, and obtained compound peptide and Freamine.
2.2 in compound peptide and Freamine, add 5% cupric oxide, 7% zinc oxide, 4% manganese carbonate respectively, stir, transferring the pH value of solution value with NaOH is to leave standstill 46～54h under 4.4～5.2,20～25 ℃, filtering-depositing is collected filtrate.
2.3 the ferrous sulfate (FeSO4) of adding 7% is heated to 60～80 ℃, stirring reaction 30～80 minutes in filtrate.
2.4 before the spraying according to the purposes of product add 0.1% KI or 0.01% sodium selenite or 0.01% cobalt chloride.
2.5 in temperature is 50～300 ℃, spray-drying under the condition of normal pressure fully both must contain the chelating amino acids premix product of various trace elements after the drying.
2.6 metering packing: select the snakeskin bag airtight package of plastic foil paper bag or band plastic foil for use, weight per package is 15～25kg.
According to claim 1-2,70～80 ℃ of metallopeptide amino-acid chelate molar ratio 2: 1, pH value 6.5, reaction temperatures and reaction time 1h that soluble metallic salt and peptide or amino acid form with covalent bonds in 1: 1～1: 3 (being preferably 1: 2) ratio.And with chemical analysis and infrared spectrum chelate is studied, proved conclusively the formation of amino-acid chelate.
As mentioned above, by the fermentation manufacturing technique after creationary employing is reformed to dregs of beans, make the fermented bean dregs feed, this fermented bean dregs feed is because the ANFs in the removal dregs of beans, multinomial nutritive index is got a promotion, the dregs of beans utilization rate is improved, and the while is also promoted production of fodder efficient, and the production of fodder cost is minimized.
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|---|---|---|---|
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|---|---|---|---|
| CN2009102082255A CN102038076A (en) | 2009-10-20 | 2009-10-20 | Trace element bean-pulp fermented composite peptide chelate compound and preparation method thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696886A (en) * | 2012-06-11 | 2012-10-03 | 武汉大学 | Feed additive and preparation method thereof |
| CN102972854A (en) * | 2012-12-17 | 2013-03-20 | 青岛根源生物技术集团有限公司 | Production device and method for producing feed soybean meal by utilizing anaerobe fermentation |
| CN104522294A (en) * | 2014-11-27 | 2015-04-22 | 苏州嘉禧萝生物科技有限公司 | Mixed bacteria composition for fermentation of soybean meal and soybean meal fermented feed |
| CN104585551A (en) * | 2015-02-02 | 2015-05-06 | 贵阳双胞胎饲料有限公司 | Pig feed additive for effectively improving intestinal health and enhancing immunity |
| CN106811437A (en) * | 2017-04-05 | 2017-06-09 | 新疆金兰德泰环保科技有限公司 | A kind of composite bacteria agent and application |
| CN114774500A (en) * | 2022-04-26 | 2022-07-22 | 成都蜀星饲料有限公司 | Polypeptide and preparation method thereof, organic composite trace element and preparation method thereof |
-
2009
- 2009-10-20 CN CN2009102082255A patent/CN102038076A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696886A (en) * | 2012-06-11 | 2012-10-03 | 武汉大学 | Feed additive and preparation method thereof |
| CN102972854A (en) * | 2012-12-17 | 2013-03-20 | 青岛根源生物技术集团有限公司 | Production device and method for producing feed soybean meal by utilizing anaerobe fermentation |
| CN102972854B (en) * | 2012-12-17 | 2015-06-03 | 青岛根源生物技术集团有限公司 | Production device and method for producing feed soybean meal by utilizing anaerobe fermentation |
| CN104522294A (en) * | 2014-11-27 | 2015-04-22 | 苏州嘉禧萝生物科技有限公司 | Mixed bacteria composition for fermentation of soybean meal and soybean meal fermented feed |
| CN104585551A (en) * | 2015-02-02 | 2015-05-06 | 贵阳双胞胎饲料有限公司 | Pig feed additive for effectively improving intestinal health and enhancing immunity |
| CN106811437A (en) * | 2017-04-05 | 2017-06-09 | 新疆金兰德泰环保科技有限公司 | A kind of composite bacteria agent and application |
| CN106811437B (en) * | 2017-04-05 | 2020-05-12 | 新疆金兰德一泰环保科技有限公司 | Complex microbial inoculant and application thereof |
| CN114774500A (en) * | 2022-04-26 | 2022-07-22 | 成都蜀星饲料有限公司 | Polypeptide and preparation method thereof, organic composite trace element and preparation method thereof |
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Application publication date: 20110504 |