Measure the method and the device of passive smoking harmfulness
Technical field
The present invention relates to a kind of measuring method and device, be specifically related to measure the method and the device of passive smoking harmfulness.
Background technology
Smoking is important component parts of modern people's life as a kind of habits and customs that very long history is arranged.But the harm of smoking health also is the formula of industry.Along with the raising of people's living standard, smoking more and more receives publicity to the influence of health of people.Especially passive smoking, just second-hand-cigarette is also very important to the harm that human body causes.For the active harmfulness of smoking, a lot of method of testings are arranged, mainly can take the measurement of the objectionable impurities in the cigarette and the relation of smoker's state are measured, but for passive smoking harmfulness, difficult to measure.This is that the harmfulness power of various objectionable constituent is also unknown because cigarette smoke is a complicated ingredient; And the cigarette smoke toxic action is the embodiment of multiple harmful substances combined action, but not the effect of certain or certain several objectionable constituent.Therefore, need to set up and the method and apparatus of measuring passive smoking harmfulness.On November 06th, 2002 is disclosed, application number is: 02113725.0, name is called: in the Chinese patent application of " a kind of method and device thereof of measuring passive smoking harmfulness ", disclose a kind of principle of utilizing the contamination of animal via respiratory tract, the toxic reaction of experiment with measuring animal in the flue gas environment, just measure the reaction of passive smoking, thereby infer the method and apparatus of measuring passive smoking harmfulness.But the deficiency of the prior art is to after producing smoking-simulating by a negative pressure air pump flue gas directly being fed the contamination cylinder, concentration to the cigarette smoke granule phase substance can not accurately be controlled and measure, measure in the passive smoking harmfulness and be easy to generate the sum of errors mistake, and structure is comparatively complicated.
Summary of the invention
The present invention has overcome the deficiencies in the prior art, and the method and the device of the mensuration passive smoking harmfulness of the concentration that can accurately measure and control cover inner wrap strip flue gas granule phase substance is provided.
For solving above-mentioned technical matters, the present invention by the following technical solutions:
A kind of method of measuring passive smoking harmfulness, according to following steps:
Step 1, experimental animal is placed in the airtight contamination cover together with rearging cage;
The animal per minute ventilation of the concentration of step 2, the cigarette smoke granule phase substance kept as required and test usefulness is set the flow of air inlet;
Step 3, light cigarette, continue to send into the mixed gas of air and flue gas by the air inlet gas circuit;
The concentration of step 4, monitoring contamination cover inner wrap strip flue gas granule phase substance, when the concentration of contamination cover inner wrap strip flue gas granule phase substance arrives setting concentration, by the mixed gas of exhaust gas circuit deaeration and flue gas, interior oxygen concentration is covered in the maintenance contamination, carbonomonoxide concentration is relative constant with flue gas granule phase substance concentration;
Step 5, allow experimental animal life in contamination cover, the viewing test reaction of animals calculates the cigarette smoke granule phase substance amount of experimental animal suction, obtains the harmfulness of the passive smoking of experimental animal.
The method of measuring passive smoking harmfulness further technical scheme is that the concentration of contamination cover inner wrap strip flue gas granule phase substance equals to reach the required cigarette number of predetermined concentration and multiply by the volume of the content of single cigarette flue gas granule phase substance divided by the contamination cover in the step 4.
The method of measuring passive smoking harmfulness further technical scheme is that the method for calculating the amount of the cigarette smoke granule phase substance that animal used as test sucks in the step 5 is to set concentration to multiply by the animal used as test throughput and multiply by exposure duration.
The method of mensuration passive smoking harmfulness further technical scheme is that a plurality of experimental animals carry out step 1 to step 5 separately at difference contamination cover respectively.
The method of mensuration passive smoking harmfulness further technical scheme is that the contamination cover is transparent.
The method of mensuration passive smoking harmfulness further technical scheme is that the contamination cover is glass or organic glass.
The technical scheme of measuring the device of passive smoking harmfulness among the present invention is: cigarette smoke air admission hole, air inlet hole, air inlet peristaltic pump, contamination are covered between injection well and the contamination cover and are communicated with by rubber catheter; Experimental animal is placed in the contamination cover together with rearging cage; Be communicated with by rubber catheter between contamination cover, contamination cover vent port, exhaust peristaltic pump, the dusty gas vent port; Fan in the contamination cover can be rapidly with gas mixing in the contamination cover; Many gas detecting instruments in the contamination cover are monitored oxygen concentration, carbonomonoxide concentration and flue gas granule phase substance concentration in real time, thereby and control air inlet peristaltic pump, exhaust peristaltic pump realize that oxygen abundance, carbonomonoxide concentration in the contamination cover are relative constant with flue gas granule phase substance concentration.
The device of passive smoking harmfulness further technical scheme is that the contamination cover is transparent.
The device of passive smoking harmfulness further technical scheme be contamination cover be glass or organic glass.
The device of passive smoking harmfulness further technical scheme is that experimental animal, rearging cage are two or more.
Compared with prior art, the invention has the beneficial effects as follows the concentration that can accurately measure and control cover inner wrap strip flue gas granule phase substance, it is exquisite more to measure passive smoking harmfulness, and device is simpler simultaneously.
Description of drawings
The structural representation of Fig. 1 for installing among the present invention.
Embodiment
The present invention is further elaborated below in conjunction with accompanying drawing.
As shown in Figure 1, measure the device of passive smoking harmfulness, cigarette smoke air admission hole 6, air inlet hole 12, air inlet peristaltic pump 2, contamination are covered between injection well 7 and the contamination cover 1 and are communicated with by rubber catheter; Experimental animal 4 is placed in the contamination cover 1 together with rearging cage 5; Be communicated with by rubber catheter between contamination cover 1, contamination cover vent port 8, exhaust peristaltic pump 3, the dusty gas vent port 9; Fan 11 in the contamination cover 1 can be rapidly with gas mixing in the contamination cover 1; Many gas detecting instruments 10 in the contamination cover 1 are monitoring oxygen concentration, carbonomonoxide concentration and flue gas granule phase substance concentration in real time, thereby and control air inlet peristaltic pump 2, exhaust peristaltic pump 3 realize that oxygen abundance, carbonomonoxide concentration in the contamination cover 1 are relative constant with flue gas granule phase substance concentration.Contamination cover 1 is transparent organic glass.In the contamination cover 1 two rearging cages 5 are arranged.
A plurality of then experimental animals 4 are respectively in rearging cage 5 is placed on airtight contamination cover 1 together; Carry out step 6 simultaneously and arrive step 9:
The animal per minute ventilation of the concentration of step 6, the cigarette smoke granule phase substance kept as required and test usefulness is set the flow of air inlet;
Step 7, light cigarette, continue to send in the contamination cover 1 by cigarette smoke air admission hole 6,12 Congjiang, air inlet hole contamination cover injection well 7 by air inlet peristaltic pump 2; Fan 11 in the contamination cover 1 can be rapidly with gas mixing in the contamination cover 1;
Many gas detecting instruments 10 in step 8, the contamination cover 1 are monitoring oxygen concentration, carbonomonoxide concentration and flue gas granule phase substance concentration in real time, when the concentration of contamination cover 1 inner wrap strip flue gas granule phase substance arrives setting concentration, discharge from dusty gas vent port 9 by exhaust peristaltic pump 3, dusty gas vent port 9 dusty gas of cover in 1 of will contaminating, keep oxygen concentration in the contamination cover 1, carbonomonoxide concentration relative constant with flue gas granule phase substance concentration; The concentration of contamination cover inner wrap strip flue gas granule phase substance equals to reach the required cigarette number of predetermined concentration and multiply by the volume of the content of single cigarette flue gas granule phase substance divided by the contamination cover.
Step 9, allow experimental animal 4 in contamination cover, live, animal used as test 4 feed of can in rearging cage 5, freely drinking water.The cigarette smoke granule phase substance amount that experimental animal 4 sucks is calculated in viewing test animal 4 reaction, and the cigarette smoke granule phase substance amount that experimental animal 4 sucks is to set concentration to multiply by the animal used as test throughput and multiply by the cigarette harmfulness that exposure duration obtains the passive suction of experimental animal again.
Contamination cover 1 can need design volume voluntarily according to experiment, therefore multiple cigarette can be tested simultaneously, has saved the repeatedly time of repeated experiments operation, has guaranteed the confidence level and the repeatability of experiment.