CN102021163A - Polyvinyl alcohol immobilization and microorganism preparation method - Google Patents
Polyvinyl alcohol immobilization and microorganism preparation method Download PDFInfo
- Publication number
- CN102021163A CN102021163A CN2009101834029A CN200910183402A CN102021163A CN 102021163 A CN102021163 A CN 102021163A CN 2009101834029 A CN2009101834029 A CN 2009101834029A CN 200910183402 A CN200910183402 A CN 200910183402A CN 102021163 A CN102021163 A CN 102021163A
- Authority
- CN
- China
- Prior art keywords
- polyvinyl alcohol
- solution
- add
- water
- acrylamide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The invention relates to a polyvinyl alcohol immobilization and microorganism preparation method. The simple preparation method adopts a polyvinyl alcohol immobilization technique, and utilizes a novel carrier for embedding and immobilizing enzymes/microorganisms. The particle mechanical strength is obviously improved. The embedding carrier cannot be broken easily, and has excellent chemical stability and strong antibacterial decomposability. The novel carrier is a great embedding material with long service life and good product quality.
Description
Technical field
The present invention relates to a kind of polyvinyl alcohol and microbial technique, particularly a kind of polyvinyl alcohol immobilized and microbe preparation method.
Background technology
It is a kind of water-soluble high-molecular compound that is rich in hydroxyl that polyvinyl alcohol is called for short (PVA), can produce the physical crosslinking effect with the hydroxyl material, in immobilization technology, normal employing polyvinyl alcohol is an embedding medium, but the water-soluble swelling property of polyvinyl alcohol immobilized particle is bigger, frangible, polyvinyl alcohol is a kind of high viscosity substance equally, polyvinyl alcohol and acid reaction are slower, can bond together when the solution of polyvinyl alcohol and boric acid is collided, and progressively be fused into one, granule of polyvinyl alcohol has very strong agglomeration tendency, make polyvinyl alcohol gel balling-up difficulty, influence the effectiveness and the repeat function of immobilized enzyme simultaneously.
When polyvinyl alcohol and boric acid react fixedly, three hydroxylic moieties on the boric acid react, also residual on the superpolymer gel that reaction back forms have a hydrophilic hydroxyl, make immobilization particle in application, deposit very big water-soluble swelling property, prolongation along with duration of service, intensity greatly weakens, its poor effect in actual application.Many scientific research personnel constantly go to explore, study, test polyvinyl alcohol immobilized enzyme and microbe preparation method in chemical enterprise, though obtain some progress, still exist the technical barrier that does not overcome as yet in actual applications.
Summary of the invention
The objective of the invention is to overcome above deficiency; provide a kind of preparation method simple; utilize a kind of novel enzyme/microorganism embedded immobilization to be carrier, particle physical strength height, non-friable; chemical stability is good; antimicrobial decomposability is strong, is a kind of good embedded material, its long service life; good product quality, a kind of practical polyvinyl alcohol immobilized and microbe preparation method.
The technical solution adopted for the present invention to solve the technical problems is:
One, polyvinyl alcohol, sodium alginate, acrylamide and pure water intermingling is even, dissolve through behind the heating in water bath, become polyvinyl alcohol solution;
Wherein: the polyvinyl alcohol add-on is 9.5-19%, and the sodium alginate add-on is 0.1-2.0%, and the acrylamide add-on is 0.5-9.0%, and its surplus is a water.
Two, enzyme is mixed with polyvinyl alcohol solution according to a certain percentage mutually with microbial suspension;
Three, saturated boric acid solution is added the N of 1-2% calcium chloride and 0.1-1.0%, N '---methylene-bisacrylamide prepares crosslinker solution;
Four, the mixing solutions that the 3rd step was prepared with syringe or dropper is added drop-wise in the refrigerative cross-linking agent solution, makes it form white spherical granules, and after soaking many hours therein, washs with physiological saline.
Effective effect of the present invention is:
By in polyvinyl alcohol-boric acid cross-linking process, introducing the agglomeration problem that a spot of (0.1-1.0%) sodium alginate has successfully solved granule of polyvinyl alcohol, strengthened the balling-up ability of polyvinyl alcohol gel;
By in polyvinyl alcohol-boric acid polymerization process, introducing the acrylamide polymerization reaction, make it to form the polyacrylamide reticulated structure in polyvinyl alcohol gel granule interior and surface, improved water-soluble, the swelling property of polyvinyl alcohol gel effectively.Polymerization single polymerization monomer propylene glutamine and linking agent N, the add-on of N '---methylene-bisacrylamide (BIS) is respectively 0.5-9.0% and 0.1-1.0%;
Polyvinyl alcohol immobilized technology can make method for preparing immobilized particles simple, and the particulate physical strength is significantly improved, and prolong its work-ing life.
Be in conjunction with the embodiments the present invention to be further described below:
Implementation method 1
(1) 10g polyvinyl alcohol, 0.5g sodium alginate, 6.0g acrylamide are mixed with the 100mL water, heating in water bath leaves standstill then and reduces to normal temperature to dissolving;
(2) cellulase weight in wet base 5g is suspended among the 10mL 0.9%NaCl, mixes mutually with the above-mentioned solution of 12mL;
(3) with the N of saturated boric acid solution adding 1%CaCl2 and 0.3%, N '---methylene-bisacrylamide prepares cross-linking agent solution;
(4) syringe with 0.40mmID is added drop-wise to mixed solution in (2) in the refrigerative cross-linking agent solution, makes its white ball-type globule that forms the 2mm diameter, and soaks 20h therein, washs with physiological saline then.
Implementation method 2
(1) 15g polyvinyl alcohol, 1.0g sodium alginate, 4.0g acrylamide are mixed with the 100mL water, heating in water bath leaves standstill then and reduces to normal temperature to dissolving;
(2) trichosporon wet cell weight 5g is suspended among the 12mL 0.9%NaCl, mixes mutually with the above-mentioned solution of 10.0mL;
(3) with the N of saturated boric acid solution adding 1.5-2%CaCl2 and 0.6%, N '---methylene-bisacrylamide prepares cross-linking agent solution;
(4) with the rubber suction pipe mixed solution in (2) is added drop-wise in the refrigerative cross-linking agent solution, makes its white ball-type globule that forms the 3mm diameter, and soak 24h therein, wash with physiological saline then.
According to above-mentioned implementation method, polyvinyl alcohol immobilized simple with microbe preparation method, particulate physical strength height makes polyvinyl alcohol gel balling-up piece, and is non-friable, its good product quality, long service life.
Claims (1)
1. polyvinyl alcohol immobilized and microbe preparation method comprises that following preparation method is: one, that polyvinyl alcohol, sodium alginate, acrylamide and pure water intermingling is even, dissolve through behind the heating in water bath, and become polyvinyl alcohol solution;
Wherein: the polyvinyl alcohol add-on is 9.5-19%, and the sodium alginate add-on is 0.1-2.0%, and the acrylamide add-on is 0.5-9.0%, and its surplus is a water;
Two, enzyme is mixed with polyvinyl alcohol solution according to a certain percentage mutually with microbial suspension;
Three, saturated boric acid solution is added the N of 1-2% calcium chloride and 0.1-1.0%, N '---methylene-bisacrylamide prepares crosslinker solution;
Four, the mixing solutions that the 3rd step was prepared with syringe or dropper is added drop-wise in the refrigerative cross-linking agent solution, makes it form white spherical granules, and after soaking many hours therein, washs with physiological saline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101834029A CN102021163A (en) | 2009-09-21 | 2009-09-21 | Polyvinyl alcohol immobilization and microorganism preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101834029A CN102021163A (en) | 2009-09-21 | 2009-09-21 | Polyvinyl alcohol immobilization and microorganism preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102021163A true CN102021163A (en) | 2011-04-20 |
Family
ID=43862945
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009101834029A Pending CN102021163A (en) | 2009-09-21 | 2009-09-21 | Polyvinyl alcohol immobilization and microorganism preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102021163A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103396562A (en) * | 2013-07-09 | 2013-11-20 | 西安交通大学 | Preparation method for sodium alginate-acrylamide-based hydrogel |
| CN104480098A (en) * | 2014-12-02 | 2015-04-01 | 杭州清科生物科技有限公司 | Creatine kinase immobilization method suitable for phosphocreatine enzyme-method production process |
| CN105441418A (en) * | 2015-12-11 | 2016-03-30 | 华南理工大学 | Polyvinyl alcohol immobilized microorganism gel beads and preparation method and use thereof |
| CN105885064A (en) * | 2016-04-18 | 2016-08-24 | 北京化工大学 | Toughened polyvinyl alcohol composite hydrogel and application thereof |
| CN115322501A (en) * | 2021-05-25 | 2022-11-11 | 浙江大学 | Reversible recyclable fixing agent for fixing fragile articles and fixing and extracting method |
-
2009
- 2009-09-21 CN CN2009101834029A patent/CN102021163A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103396562A (en) * | 2013-07-09 | 2013-11-20 | 西安交通大学 | Preparation method for sodium alginate-acrylamide-based hydrogel |
| CN103396562B (en) * | 2013-07-09 | 2015-07-08 | 西安交通大学 | Preparation method for sodium alginate-acrylamide-based hydrogel |
| CN104480098A (en) * | 2014-12-02 | 2015-04-01 | 杭州清科生物科技有限公司 | Creatine kinase immobilization method suitable for phosphocreatine enzyme-method production process |
| CN105441418A (en) * | 2015-12-11 | 2016-03-30 | 华南理工大学 | Polyvinyl alcohol immobilized microorganism gel beads and preparation method and use thereof |
| CN105885064A (en) * | 2016-04-18 | 2016-08-24 | 北京化工大学 | Toughened polyvinyl alcohol composite hydrogel and application thereof |
| CN115322501A (en) * | 2021-05-25 | 2022-11-11 | 浙江大学 | Reversible recyclable fixing agent for fixing fragile articles and fixing and extracting method |
| CN115322501B (en) * | 2021-05-25 | 2023-11-24 | 浙江大学 | Reversible and recyclable fixing agent for fixing fragile articles and fixing extraction method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wahba | Porous chitosan beads of superior mechanical properties for the covalent immobilization of enzymes | |
| CN101348782B (en) | Macroreticular polyvinyl alcohol bead carrier and preparation thereof | |
| CN102021163A (en) | Polyvinyl alcohol immobilization and microorganism preparation method | |
| Elçin | Encapsulation of urease enzyme in xanthan-alginate spheres | |
| CN101497880B (en) | Novel method for improving PVA immobilized microorganism | |
| CN105441418A (en) | Polyvinyl alcohol immobilized microorganism gel beads and preparation method and use thereof | |
| Li et al. | Recent progress in the development of immobilized penicillin G acylase for chemical and industrial applications: A mini‐review | |
| CN101054575B (en) | Preparation method for modified polyacrylamide immobilization cell | |
| CN104099316A (en) | Amphiphilic structure microsphere based on hydrophobic modified sodium alginate material and preparation and application | |
| CN110510760B (en) | Double-layer carbon source microsphere and preparation and application thereof | |
| CN1137991C (en) | Process for immobilizing enzyme/microbe with polyvinyl alcohol | |
| KR100684603B1 (en) | Novel hybrid immobilization method of microorganisms and bead produced therefrom | |
| CN100506985C (en) | Microbe immobilicing gel material for waste water treatment | |
| CN102250867A (en) | Poly(vinyl alcohol)-immobilized microorganism particles and preparation method thereof | |
| CN101157914B (en) | Bentonite, biological polysaccharide plural gel immobilized cell carrier and preparation method thereof | |
| JPS58187188A (en) | Immobilization method of enzymic active substance | |
| CN1970747A (en) | Process for preparing spherical immobilized cell and enzyme particulate | |
| Guo et al. | Morphology and metabolism of Ba-alginate-encapsulated hepatocytes with galactosylated chitosan and poly (vinyl alcohol) as extracellular matrices | |
| CN101691415A (en) | Method of preparing agricultural and forestal super absorbent resin | |
| WO1996002581A1 (en) | Process for producing aqueous gel, heavy-metal ion adsorbent, pigment adsorbent, microbe carrier, and carrier for enzyme immobilization | |
| CN114044925A (en) | Preparation method and application of polyvinyl alcohol gel material | |
| CN115739038A (en) | Method for treating phosphorus-containing wastewater by utilizing lanthanum-functionalized silica aerogel microsphere composite material | |
| Shousha | Novel application of Luffa cylindrica in production of fructose | |
| CN114349984B (en) | Composite hydrogel for wastewater treatment and preparation method and application thereof | |
| CN1164745C (en) | Method for immobilizing penicillin amidase and cell containing penicillin amidase |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110420 |