CN102015094B - 层析介质 - Google Patents

层析介质 Download PDF

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Publication number
CN102015094B
CN102015094B CN200980114972.0A CN200980114972A CN102015094B CN 102015094 B CN102015094 B CN 102015094B CN 200980114972 A CN200980114972 A CN 200980114972A CN 102015094 B CN102015094 B CN 102015094B
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China
Prior art keywords
particles
molecules
organisms
medium
hydrophobic
Prior art date
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CN200980114972.0A
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English (en)
Chinese (zh)
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CN102015094A (zh
Inventor
J·伯格斯特伦
G·格拉德
B-L·约翰逊
J-L·马洛伊塞尔
N·诺尔曼
T·E·塞德曼
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Cytiva Bioprocess R&D AB
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GE Healthcare Bio Sciences Corp
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
    • B01J20/28009—Magnetic properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • B01J20/285—Porous sorbents based on polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/287—Non-polar phases; Reversed phases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/3212—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3291—Characterised by the shape of the carrier, the coating or the obtained coated product
    • B01J20/3293—Coatings on a core, the core being particle or fiber shaped, e.g. encapsulated particles, coated fibers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/26—Cation exchangers for chromatographic processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
CN200980114972.0A 2008-04-22 2009-04-21 层析介质 Active CN102015094B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0800923-5 2008-04-22
SE0800923 2008-04-22
PCT/SE2009/050406 WO2009131526A1 (en) 2008-04-22 2009-04-21 Chromatography medium

Publications (2)

Publication Number Publication Date
CN102015094A CN102015094A (zh) 2011-04-13
CN102015094B true CN102015094B (zh) 2016-11-09

Family

ID=41217058

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200980114972.0A Active CN102015094B (zh) 2008-04-22 2009-04-21 层析介质

Country Status (7)

Country Link
US (4) US20110045574A1 (enExample)
EP (1) EP2268393A4 (enExample)
JP (1) JP5643747B2 (enExample)
CN (1) CN102015094B (enExample)
AU (1) AU2009238686B2 (enExample)
DE (1) DE09735164T1 (enExample)
WO (1) WO2009131526A1 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5643747B2 (ja) 2008-04-22 2014-12-17 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ クロマトグラフィー媒体
US20110142863A1 (en) * 2009-12-16 2011-06-16 Millipore Corporation Flow through purification processes for large biomolecules
IN2012DN06243A (enExample) * 2010-02-19 2015-09-25 Ge Healthcare Bio Sciences Ab
WO2012134381A1 (en) * 2011-03-31 2012-10-04 Ge Healthcare Bio-Sciences Ab Method and means for sample preparation
US9260536B2 (en) 2011-07-22 2016-02-16 Stellan Hjerten Capture of pathogenic and non-pathogenic biopolymers and bioparticles
WO2013130001A1 (en) * 2012-02-29 2013-09-06 Ge Healthcare Bio-Sciences Ab Method for endotoxin removal
US10041050B2 (en) 2012-02-29 2018-08-07 Ge Healthcare Bioprocess R&D Ab Method for endotoxin removal
US9782468B2 (en) 2013-05-20 2017-10-10 Shantha Biotechnics Private Limited Purification of polysaccharide protein conjugates
DK3043904T3 (en) 2013-09-09 2018-03-26 Lab On A Bead Ab MAGNETIC PARTICLES FOR SEPARATION AND ANALYSIS OF MOLECULES AND CELLS AND PROCEDURE FOR MANUFACTURING THESE PARTICLES
WO2015137860A1 (en) * 2014-03-14 2015-09-17 Ge Healthcare Bio-Sciences Ab Separation matrices for purification of biological particles
US10626164B2 (en) 2014-07-25 2020-04-21 Csl Limited Purification of VWF
GB201506117D0 (en) 2015-04-10 2015-05-27 Ge Healthcare Bio Sciences Ab Method for chromatography
GB201506113D0 (en) 2015-04-10 2015-05-27 Ge Healthcare Bio Sciences Ab Method for chromatography
EP3353193A4 (en) * 2015-09-22 2019-04-03 Sanofi Pasteur Biologics, LLC CLEANING OF THE RESPIRATORY SYNZYTIAL VIRUS
ES2986551T3 (es) * 2016-04-06 2024-11-11 Cytiva Bioprocess R & D Ab Matriz de cromatografía
EP4574227A3 (en) 2016-09-29 2025-09-03 Bio-Rad Laboratories, Inc. Protein-nanoparticle conjugate purification methods
US10814305B2 (en) 2016-09-29 2020-10-27 Bio-Rad Laboratories, Inc. Agarose-filled ceramic apatite
KR102071507B1 (ko) * 2018-03-26 2020-01-30 국민대학교산학협력단 바이오센서용 하이드로겔 구조체, 이를 포함하는 바이오센서, 및 상기 바이오센서용 하이드로겔 구조체의 제조방법
GB201806736D0 (en) * 2018-04-25 2018-06-06 Ge Healthcare Bioprocess R&D Ab Method for virus purification
JP7577538B2 (ja) 2020-02-19 2024-11-05 バイオ－ラッド ラボラトリーズ インコーポレーティッド ポリマー充填クロマトグラフィー樹脂を調製する方法
CN111420644A (zh) * 2020-03-27 2020-07-17 北京石油化工学院 一种复合型混合模式聚合物层析介质的制备方法
CN113416235B (zh) * 2021-06-24 2023-04-25 苏州赛分科技股份有限公司 用于纯化分离病毒类抗原的液相色谱法
US20240198316A1 (en) * 2021-05-31 2024-06-20 Suzhou Sepax Technologies, Inc. Synthetic polymeric porous medium with hierarchical multiple layer structure, its design, synthesis, modification, and liquid chromatographic applications
US20260028597A1 (en) 2022-12-27 2026-01-29 Cytiva Bioprocess R&D Ab Purification of biological particles
EP4694993A1 (en) 2023-04-12 2026-02-18 Cytiva BioProcess R&D AB A chromatography system, use thereof, and a method for separating enveloped or membranous biological particles

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6531523B1 (en) * 2000-10-10 2003-03-11 Renal Tech International, Llc Method of making biocompatible polymeric adsorbing material for purification of physiological fluids of organism
CN1528511A (zh) * 2003-09-27 2004-09-15 南开大学 内毒素吸附剂及其制备方法
CN101035439A (zh) * 2004-08-20 2007-09-12 普洛麦提生命科学有限公司 通过亲和色谱法进行的连续蛋白质分离和提纯方案

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61287444A (ja) * 1985-06-13 1986-12-17 Toyo Soda Mfg Co Ltd 二重構造担体
DK165090D0 (da) 1990-07-09 1990-07-09 Kem En Tec As Konglomererede partikler
SE9101149D0 (sv) 1991-04-17 1991-04-17 Pharmacia Lkb Biotech Beads for down stream processing
SE9700768D0 (sv) 1997-03-04 1997-03-04 Pharmacia Biotech Ab Förfarande för att introducera funktionalitet
SE9700769D0 (sv) * 1997-03-04 1997-03-04 Pharmacia Biotech Ab Matriser för separation och separation som utnyttjar matriserna
SE9904272D0 (sv) * 1999-11-25 1999-11-25 Amersham Pharm Biotech Ab A method for selective removal of a substance from samples containing compounds having nucleic acid structure
US20030125656A1 (en) * 2001-12-31 2003-07-03 Vadim Davankov Hemo-and biocompatible beaded polymeric material for purification of physiological fluids of organism, method of producing the material, as well as method of and device for purification of physiological fluids of organism with use of the material
SE0202551D0 (sv) * 2002-08-27 2002-08-27 Amersham Biosciences Ab Chromatographic two-layer particles
US20050079984A1 (en) 2003-10-08 2005-04-14 Miles Samuel Lee Method for stripping cured paint from plastic, steel, aluminum, brass, magnesium and non-ferrous substrates with surfactants low in volatile organic compounds
JP2007522807A (ja) * 2004-02-18 2007-08-16 アプレラ コーポレイション 高分子電解質コーティングされたサイズ排除イオン交換粒子
US20050181378A1 (en) * 2004-02-18 2005-08-18 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
SG131016A1 (en) * 2005-09-19 2007-04-26 Millipore Corp Asymmetric porous adsorptive bead
SE0502485L (sv) * 2005-11-09 2007-05-10 Peter Viberg Partiklar
WO2007064281A1 (en) * 2005-12-02 2007-06-07 Ge Healthcare Bio-Sciences Ab Hydrophobic interaction chromatography
US10562007B2 (en) * 2007-07-25 2020-02-18 Ge Healthcare Bioprocess R&D Ab Separation matrix
US8372286B2 (en) 2008-02-05 2013-02-12 Ge Healthcare Bio-Sciences Ab Method for production of separation media
JP5643747B2 (ja) 2008-04-22 2014-12-17 ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ クロマトグラフィー媒体
KR100889904B1 (ko) 2008-12-16 2009-03-23 주식회사 효민산업 수중직립 관절이완 및 척추교정 장치

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6531523B1 (en) * 2000-10-10 2003-03-11 Renal Tech International, Llc Method of making biocompatible polymeric adsorbing material for purification of physiological fluids of organism
CN1528511A (zh) * 2003-09-27 2004-09-15 南开大学 内毒素吸附剂及其制备方法
CN101035439A (zh) * 2004-08-20 2007-09-12 普洛麦提生命科学有限公司 通过亲和色谱法进行的连续蛋白质分离和提纯方案

Also Published As

Publication number Publication date
WO2009131526A1 (en) 2009-10-29
CN102015094A (zh) 2011-04-13
EP2268393A4 (en) 2013-08-14
US20220266170A1 (en) 2022-08-25
AU2009238686B2 (en) 2014-08-28
DE09735164T1 (de) 2024-06-20
US20200101398A1 (en) 2020-04-02
JP2011518337A (ja) 2011-06-23
US10493380B2 (en) 2019-12-03
US11439926B2 (en) 2022-09-13
JP5643747B2 (ja) 2014-12-17
EP2268393A1 (en) 2011-01-05
US20170050121A1 (en) 2017-02-23
AU2009238686A1 (en) 2009-10-29
US20110045574A1 (en) 2011-02-24

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