CN102015094B - 层析介质 - Google Patents

层析介质 Download PDF

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Publication number
CN102015094B
CN102015094B CN200980114972.0A CN200980114972A CN102015094B CN 102015094 B CN102015094 B CN 102015094B CN 200980114972 A CN200980114972 A CN 200980114972A CN 102015094 B CN102015094 B CN 102015094B
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China
Prior art keywords
particles
molecules
organisms
medium
hydrophobic
Prior art date
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CN200980114972.0A
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English (en)
Chinese (zh)
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CN102015094A (zh
Inventor
J·伯格斯特伦
G·格拉德
B-L·约翰逊
J-L·马洛伊塞尔
N·诺尔曼
T·E·塞德曼
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Cytiva Bioprocess R&D AB
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GE Healthcare Bio Sciences Corp
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
    • B01J20/28009—Magnetic properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • B01J20/285—Porous sorbents based on polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/287—Non-polar phases; Reversed phases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B01J20/3212—Polymeric carriers, supports or substrates consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3291—Characterised by the shape of the carrier, the coating or the obtained coated product
    • B01J20/3293—Coatings on a core, the core being particle or fiber shaped, e.g. encapsulated particles, coated fibers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/26—Cation exchangers for chromatographic processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
CN200980114972.0A 2008-04-22 2009-04-21 层析介质 Active CN102015094B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0800923-5 2008-04-22
SE0800923 2008-04-22
PCT/SE2009/050406 WO2009131526A1 (en) 2008-04-22 2009-04-21 Chromatography medium

Publications (2)

Publication Number Publication Date
CN102015094A CN102015094A (zh) 2011-04-13
CN102015094B true CN102015094B (zh) 2016-11-09

Family

ID=41217058

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200980114972.0A Active CN102015094B (zh) 2008-04-22 2009-04-21 层析介质

Country Status (7)

Country Link
US (4) US20110045574A1 (enExample)
EP (1) EP2268393A4 (enExample)
JP (1) JP5643747B2 (enExample)
CN (1) CN102015094B (enExample)
AU (1) AU2009238686B2 (enExample)
DE (1) DE09735164T1 (enExample)
WO (1) WO2009131526A1 (enExample)

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AU2009238686B2 (en) 2008-04-22 2014-08-28 Cytiva Bioprocess R&D Ab Chromatography medium
US20110142863A1 (en) * 2009-12-16 2011-06-16 Millipore Corporation Flow through purification processes for large biomolecules
EP2536499A4 (en) * 2010-02-19 2013-08-14 Ge Healthcare Bio Sciences Ab METHOD FOR PRODUCING CHROMATOGRAPHIC MEDIA
EP2691767B1 (en) * 2011-03-31 2016-03-23 GE Healthcare Bio-Sciences AB Method for sample preparation
US9260536B2 (en) 2011-07-22 2016-02-16 Stellan Hjerten Capture of pathogenic and non-pathogenic biopolymers and bioparticles
WO2013130001A1 (en) * 2012-02-29 2013-09-06 Ge Healthcare Bio-Sciences Ab Method for endotoxin removal
US10041050B2 (en) 2012-02-29 2018-08-07 Ge Healthcare Bioprocess R&D Ab Method for endotoxin removal
BR112015029051A2 (pt) 2013-05-20 2017-07-25 Shantha Biotechnics Private Ltd processos para a purificação de um conjugado de proteínas de polissacarídeos e para a fabricação de uma vacina
CN105980046B (zh) * 2013-09-09 2018-04-03 微球实验公司 使用具有磁性性质的颗粒的新诊断测定
CN106102903A (zh) * 2014-03-14 2016-11-09 通用电气医疗集团生物工艺研发股份公司 用于生物颗粒纯化的分离基质
US10626164B2 (en) 2014-07-25 2020-04-21 Csl Limited Purification of VWF
GB201506113D0 (en) 2015-04-10 2015-05-27 Ge Healthcare Bio Sciences Ab Method for chromatography
GB201506117D0 (en) 2015-04-10 2015-05-27 Ge Healthcare Bio Sciences Ab Method for chromatography
US20180273909A1 (en) * 2015-09-22 2018-09-27 Sanofi Pasteur Biologics, Llc Purification of respiratory syncytial virus
WO2017174422A1 (en) * 2016-04-06 2017-10-12 Ge Healthcare Bioprocess R&D Ab Chromatography matrix
US10934366B2 (en) 2016-09-29 2021-03-02 Bio-Rad Laboratories, Inc. Protein-nanoparticle conjugate purification methods
WO2018063980A1 (en) 2016-09-29 2018-04-05 Bio-Rad Laboratories, Inc. Agarose-filled ceramic apatite
KR102071507B1 (ko) * 2018-03-26 2020-01-30 국민대학교산학협력단 바이오센서용 하이드로겔 구조체, 이를 포함하는 바이오센서, 및 상기 바이오센서용 하이드로겔 구조체의 제조방법
GB201806736D0 (en) * 2018-04-25 2018-06-06 Ge Healthcare Bioprocess R&D Ab Method for virus purification
CN115135392A (zh) 2020-02-19 2022-09-30 生物辐射实验室股份有限公司 制备聚合物填充的色谱树脂的方法
CN111420644A (zh) * 2020-03-27 2020-07-17 北京石油化工学院 一种复合型混合模式聚合物层析介质的制备方法
WO2022253175A1 (en) * 2021-05-31 2022-12-08 Suzhou Sepax Technologies, Inc Synthetic polymeric porous medium with hierarchical multiple layer structure, its design, synthesis, modification, and liquid chromatographic applications
CN113416235B (zh) * 2021-06-24 2023-04-25 苏州赛分科技股份有限公司 用于纯化分离病毒类抗原的液相色谱法
CN120435334A (zh) 2022-12-27 2025-08-05 思拓凡生物工艺研发有限公司 生物颗粒的纯化
KR20250172638A (ko) 2023-04-12 2025-12-09 씨티바 바이오프로세스 알＆디 에이비 크로마토그래피 시스템, 그의 용도, 및 외피보유 또는 멤브레인성 생물학적 입자를 분리하는 방법

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CN1528511A (zh) * 2003-09-27 2004-09-15 南开大学 内毒素吸附剂及其制备方法
CN101035439A (zh) * 2004-08-20 2007-09-12 普洛麦提生命科学有限公司 通过亲和色谱法进行的连续蛋白质分离和提纯方案

Also Published As

Publication number Publication date
EP2268393A1 (en) 2011-01-05
US10493380B2 (en) 2019-12-03
AU2009238686A1 (en) 2009-10-29
EP2268393A4 (en) 2013-08-14
WO2009131526A1 (en) 2009-10-29
CN102015094A (zh) 2011-04-13
US20220266170A1 (en) 2022-08-25
JP2011518337A (ja) 2011-06-23
DE09735164T1 (de) 2024-06-20
US20170050121A1 (en) 2017-02-23
AU2009238686B2 (en) 2014-08-28
US20110045574A1 (en) 2011-02-24
US20200101398A1 (en) 2020-04-02
JP5643747B2 (ja) 2014-12-17
US11439926B2 (en) 2022-09-13

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