CN102002439A - Medicinal and edible plant chimonanthus grammatus essential oil, and preparation method and application thereof - Google Patents
Medicinal and edible plant chimonanthus grammatus essential oil, and preparation method and application thereof Download PDFInfo
- Publication number
- CN102002439A CN102002439A CN2010105479090A CN201010547909A CN102002439A CN 102002439 A CN102002439 A CN 102002439A CN 2010105479090 A CN2010105479090 A CN 2010105479090A CN 201010547909 A CN201010547909 A CN 201010547909A CN 102002439 A CN102002439 A CN 102002439A
- Authority
- CN
- China
- Prior art keywords
- essential oil
- wax plum
- prominent holder
- prominent
- dimethyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a medicinal and edible plant chimonanthus grammatus essential oil, and a preparation method and application thereof. The chimonanthus grammatus essential oil is extracted by a vapor distillation method; 53 components are detected by gas-phase chromatogram-mass spectrum combined technology; the identified components occupy 99.99 percent of total peak area of a chromatogram of volatile oil; and the main components of the essential oil are (E)-3-(4,8-dimethyl-3,7-nonene)-furan, acetyl borneol ester, 6,6-dimethyl-3-methylene-bicyclo[3.1.1] heptane, tetrahydro solasodine, ledene alcohol, 1,5-dimethyl-1-ethenyl-4-hexenyl butyrate and the like which account for 49.87 percent of the total components of the volatile oil. The essential oil has higher antibacterial activity, wider antibacterial spectrum and high anti-oxidation activity; and can be used as a natural antibacterial and antioxidant active ingredient for preparing cosmetic additives, food additives, medicinal products, health-care products and the like.
Description
Technical field
The present invention relates to the prominent holder of a kind of medicine food dual purpose plant wax plum essential oil preparation method and its usage, adopt steam distillation extraction separation essential oil, biological activity assay proves that this derived essential oil has well antibacterial and anti-oxidant activity, has the good purposes in fields such as being applied to cosmetics additive, foodstuff additive, medicinal and healthcare products.
Background technology
Microbial contamination is most threatening in makeup, a foodstuffs industry major issue always, it not only produces great effect in production and quality examination process, the more important thing is the consumer health is caused a significant threat that therefore, the interpolation of sanitas is inevitable.But because the interpolation of a large amount of Chemical Preservatives, when suppressing bacteria breed, its safety in utilization also exists very big query.To health demand enhanced today day by day, the makeup and the related products thereof that use as Pursuit for Aesthetics, should reduce it as much as possible to human consumer's health threat, food safety also is a major issue of people's growing interest simultaneously, and therefore deriving from natural natural bacteriostatic composition is only the key point that addresses this problem.Plants essential oil is as a kind of active skull cap components, and its inherent fragrance and its various biological activitys that have are that it becomes the first-selection that addresses this problem.
Essential oil (Essential oils) claims volatile oil (Volatile oils) again, be present in a class in the plant have aromatic odour, can with wet distillation come out and with the general name of the not miscible volatility oily composition of water.The interpolation that the interpolation that studies show that essential oil can effectively replace or reduce Chemical Preservative guarantees the validity of protective system simultaneously, medicinal health effects such as that many in addition plants essential oils also possess simultaneously is anti-oxidant, anti-inflammatory, antiviral, antitumor, antipyretic-antalgic, expelling phlegm for arresting cough, these activity make it give full play to its exclusive effect from produce the too many levels that the human consumer uses and progressively approved by people in makeup, foodstuffs industry.
Wax plum platymiscium is under the jurisdiction of Laurales, Calycanthaceae, and its ornamental value gets most of the attention in China always, and simultaneously, wax plum platymiscium also is China's traditional Chinese medicine, has high medicinal, edibleness.There are some researches prove in this platymiscium body and contained abundant bioactive ingredients, have relieving cough and reducing sputum, anti-inflammatory is analgesic, the pharmacological effect of antibiotic, anti-oxidant, antiviral, step-down, analgesia, strengthening immunity, its main active ingredient is essential oil, alkaloid and Flavonoid substances.Prominent holder wax plum belongs to a novel species for the wax plum, is the domestic peculiar species in Jiangxi, and because of its areal distribution is narrow and small and rarely known by the people, its development and use are in the starting stage, and the natural radioactivity development of resources still belongs to blank.According to existing research method derived essential oil has been carried out preliminary study, find that it has stronger bacteriostatic activity, wider antimicrobial spectrum and good antioxidant activity, show that this plant resources has unlimited potentiality to be exploited, be expected to become the natural bacteriostatic of makeup, foodstuff additive, medicinal and healthcare products etc., the novel resource of oxidation-resistant active ingredient.
Summary of the invention
The object of the invention is, the preparation method and the purposes of the prominent holder of a kind of medicine food dual purpose plant wax plum essential oil are provided, by a series of pharmacology activity researchs, confirm that it has stronger bacteriostatic activity and anti-oxidant activity, can be used as the purposes of anticorrosion, the antioxidant addn of series such as makeup and food.
It is simple that the present invention extracts the extracting method flow process, easy to operate, with low cost, and the yield height is safe and reliable, and substance environment pollutes, and products obtained therefrom has higher purity, is fit to characteristics such as big production, is a kind of good extraction and separation method.
A kind of prominent holder of medicine food dual purpose plant wax plum essential oil that utilizes steam distillation to extract of the present invention, choose prominent holder in autumn wax plum and do not have the complete fresh leaf of scab, gather, clean, natural air drying, pulverize, wet distillation, oily water separation can obtain prominent holder wax plum essential oil, oil yield is 0.7-1.5%, its main chemical compositions is (E)-3-(4,8-dimethyl-3,7-nonene base)-furans 13.81%, acetyl norbornene ester 12.66%, 6,6-dimethyl-3-methylene radical-dicyclo [3.1.1] heptane 7.06%, tetrahydrochysene solasodine 5.93%, loudspeaker enol 5.32%, 1,5-dimethyl-1-vinyl-4 butyric acid hexene ester 5.09% composition such as grade accounts for 49.87% of total volatile oil composition.This essential oil main component is an olefines, wherein is mainly terpene substances, and wherein geranic acid, myrcene etc. are important spice source, and cadinol plays an important role at aspects such as promoting human body hormone secretion, the thyroid infiltration of raising.
The prominent holder of described medicine food dual purpose plant wax plum extracts essential oil method, its extraction step is:
(1) select the complete disease-free fresh blade of prominent holder wax plum in autumn for use, remove impurity, use flushing with clean water 1-3 time, natural air drying 12-48 hour, crushing screening 20-60 order, sealing is preserved standby;
(2) take by weighing a certain amount of prominent holder wax plum powder in round-bottomed flask, add water 5-10 and doubly measure, wet distillation 6-8 hour;
(3) distillate is loaded in the separating funnel, left standstill 0.5 hour, reclaim the faint yellow transparent oily liquid in upper strata;
(4) remove moisture with anhydrous sodium sulfate drying, be prominent holder wax plum essential oil, yield is: 0.7%-1.5%, and in brown air-tight bottle-20 ℃ preservation.
Described prominent holder wax plum essential oil, the fungistatic effect that ten strains is tried bacterium is very obvious, and minimum inhibitory concentration and minimal bactericidal concentration are all between 2-16mg/ml.Simultaneously it is carried out reductibility and removes free radical activity detection, DPPH free radical scavenging activity IC
50=12.145mg/ml.Cosmetics preservative challenge test shows, this essential oil is tried bacterium to three kinds of inoculation can play the good sterilization preservative activity in the test period.General performance is that this essential oil has broad-spectrum antibacterial effect and good antioxidant activity.
It is active and than the strong anti-oxidation activity that described prominent holder wax plum essential oil has good broad-spectrum antibacterial, this essential oil fragrance is pure and fresh pleasant, be suitable as very much cosmetics additive, the effect of its performance not only can be used as antipathogenic composition, also comprises the antidotal natural effective active composition of degerming.The present invention is a lipotropy pale yellow oily liquid body, good consistency is arranged in the makeup organic phase, thereby can give full play to its performance.Add to them in makeup or as foodstuff additive, can bring into play the natural anticorrosion effect, also can be used as activeconstituents, alternative or reduce the consumption of Chemical Preservative, thereby further cater to the demand of people, have higher economic worth and vast market prospect makeup, the better security of food.
Described prominent holder wax plum essential oil is as the purposes of antibacterial, anticorrosion, the antioxidant addn of preparation makeup and articles for washing.
Described prominent holder wax plum essential oil is as the purposes of preparation foodstuff additive.
Described prominent holder wax plum essential oil is as the purposes of preparation healthcare products.
The present invention adopts chromatography of gases-mass spectrometric hyphenated technique to be:
GC conditions: HP-5MS quartz capillary column (0.25mm*30m*0.25um), press 8.12psi before the carrier gas 99.9995%He, post, 60 ℃ of column temperatures, 4 ℃/min is raised to 280 ℃ behind the reservation 2min, keeps 20min, the sample introduction temperature is 280 ℃, sample size 0.2 μ l, splitting ratio 50: 1;
The mass spectrum condition: ion source is EI, electron energy 70eV, 180 ℃ of ion source temperatures, 22 ℃ of transmission line temperature, sweep limit 50-550amu, solvent delay 3min;
Data processing and mass spectrum retrieval: sample advances gas chromatography-mass spectrometry analysis, and each separated portion adopts Nbs, Nist, Wiley spectrum storehouse retrieval-by-unification, and reference is confirmed, quantitatively adopts the chromatographic peak area normalization method.
Below in conjunction with the biological activity assay of prominent holder wax plum essential oil, further verify biological activity of the present invention and effect thereof.
(1) prominent holder wax plum essential oil sample of the present invention is to gram-positive microorganism (intestinal bacteria, Pseudomonas aeruginosa, Corynebacterium diphtheriae, dysentery bacterium); The antibacterial activity in vitro experimental technique and the result of Gram-negative bacteria (streptococcus aureus, subtilis, Sarcina lutea, bacillus thuringiensis, gas bacillus):
Testing method: with filter paper method test fungistatic effect; Pass through the minimum inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) of this volatile oil of constant broth dilution method determination again.Substratum is respectively beef-protein medium and broth culture.
Show that by filter paper method bacteriostatic test certain density prominent holder wax plum essential oil sample all has tangible bacteriostatic action to this ten strains bacterium.Minimum inhibitory concentration MIC and minimum bactericidal concentration MBC are between 2-16mg/ml.Experiment shows that prominent holder wax plum essential oil tool is all inhibited to Gram-positive and negative bacterium, and the broad-spectrum antibacterial effect is arranged, and the results are shown in Table 1.
The prominent holder of table 1 wax plum essential oil is measured MIC and the MBC that is tried bacterium:
(2) adopt reductibility, the test of DPPH free radical scavenging activity that the oxidation-resistance of prominent holder wax plum essential oil sample of the present invention is estimated:
Testing method:
Reductibility is measured: add sample liquid 1.0mL, phosphoric acid buffer (0.2M, pH6.6) 2.5mL, 1% Tripotassium iron hexacyanide 2.5mL in the test tube.Behind 50 ℃ of water-bath 20min, add 10% trichoroacetic acid(TCA) 2.5mL, mixing is centrifugal.Get supernatant liquor 2.5mL and add distilled water 2.5mL and 0.1% iron trichloride 0.5mL.Measure absorbance behind the 10min at the 700nm place.The big more expression reducing power of absorbance is strong more.
The test of DPPH free radical scavenging activity: accurately take by weighing 10mgDPPH solution and dehydrated alcohol, and be settled to 250mL, then DPPH concentration is 0.1mmol/L, keeps in Dark Place.With 2mL, 5mg/mL sample and 2mL DPPH solution add in the same test tube, shake up, and measure its absorbancy with solvent as reference after placing 30min, measure absorbancy after DPPH solution and the solvent and the absorbancy after sample and the solvent simultaneously.Calculate clearance rate according to following formula, the big more resistance of oxidation of clearance rate is strong more.(Vc) makes positive control with xitix.
DPPH clearance rate %=[1-(At-Ab)]/Ac*100
In the formula: At is sample and DPPH solution absorbency;
Ac is the absorbancy after DPPH solution and the solvent;
Ab is the absorbancy after sample and the solvent.
Anti-oxidant activity detect to show that this essential oil has stronger reductibility, free radical scavenging activity its results are shown in Table 2 with table 3, IC
50=12.145mg/ml.
The prominent holder of table 2 wax plum reductibility is measured
The prominent holder of table 3 wax plum essential oil DPPH free radical scavenging activity is measured
(3) adopt the inhibitor effectiveness of the microorganism challenge experimental test essential oil of makeup to makeup:
Testing method: with reference to the method for U.S.'s makeup, toilet articles and essence association (CTFA)
Tried bacterium: intestinal bacteria (Escherichia coil), streptococcus aureus (Staphylococcus aureus), Pseudomonas aeruginosa (Pseudomonas aeruginosa).
Experimental result indicates that being tried bacteria concentration in 28 days obviously reduces, and this has further confirmed bacteriostatic activity and its validity as the makeup anticorrosive additive of this essential oil, and it the results are shown in Figure 2.
Useful achievement of the present invention:
(1) reported first of the present invention is extracted plants essential oil from prominent holder wax plum, and this essential oil has broad-spectrum antibacterial effect and good antioxidant activity.This aboundresources is the domestic endemic species in Jiangxi, distributes to concentrate, and is beneficial to development and use, and preparation technology is reasonable, feasible, is easy to realize industrialization;
(2) plants essential oil provided by the invention can be applied in the industries such as cosmetics additive, foodstuff additive, medicinal and healthcare products.
Description of drawings
To be intestinal bacteria of the present invention (Escherichia coil), streptococcus aureus (Staphylococcus aureus), Pseudomonas aeruginosa (Pseudomonas aeruginosa) containing prominent holder wax plum essential oil and do not adding growth-inhibiting figure [contain 5% prominent holder wax plum essential oil () and do not contain prominent holder wax plum essential oil (zero)] in the cosmetic base of essential oil Fig. 1.
Embodiment
Embodiment 1
(1) select the complete disease-free fresh blade of prominent holder wax plum for use, remove impurity, use flushing with clean water 3 times, natural air drying 24 hours, crushing screening 20 orders, sealing is preserved standby;
(2) take by weighing the prominent holder of 30g wax plum powder in the 500ml flask, add 250ml distilled water, wet distillation 8 hours;
(3) distillate is loaded in the separating funnel, left standstill 0.5 hour, reclaim the faint yellow transparent oily liquid in upper strata;
(4) remove moisture with anhydrous sodium sulfate drying, be prominent holder wax plum essential oil, in brown air-tight bottle-20 ℃ preservation.
The prominent holder wax plum essential oil that is obtained is as the purposes of antibacterial, anticorrosion, the antioxidant addn of preparation makeup and articles for washing or as the purposes of foodstuff additive or as the purposes of healthcare products etc.
Embodiment 2
(1) select the complete disease-free fresh blade of prominent holder wax plum for use, remove impurity, use flushing with clean water 2 times, natural air drying 48 hours, crushing screening 40 orders, sealing is preserved standby;
(2) take by weighing the prominent holder of 80g wax plum powder in the 1000ml round-bottomed flask, add water 500ml distilled water, wet distillation 7 hours;
(3) distillate is loaded in the separating funnel, left standstill 0.5 hour, reclaim the faint yellow transparent oily liquid in upper strata;
(4) remove moisture with anhydrous sodium sulfate drying, be prominent holder wax plum essential oil, in brown air-tight bottle-20 ℃ preservation.
The prominent holder wax plum essential oil that is obtained is as the purposes of antibacterial, anticorrosion, the antioxidant addn of preparation makeup and articles for washing or as the purposes of foodstuff additive or as the purposes of healthcare products etc.
Embodiment 3
(1) select the complete disease-free fresh blade of prominent holder wax plum for use, remove impurity, use flushing with clean water 3 times, natural air drying 36 hours, crushing screening 60 orders, sealing is preserved standby;
(2) take by weighing the prominent holder of 50g wax plum powder in the 500ml flask, add 250ml distilled water, wet distillation 6 hours;
(3) distillate is loaded in the separating funnel, left standstill 0.5 hour, reclaim the faint yellow transparent oily liquid in upper strata;
(4) remove moisture with anhydrous sodium sulfate drying, be prominent holder wax plum essential oil, in brown air-tight bottle-20 ℃ preservation.
The prominent holder wax plum essential oil that is obtained is as the purposes of antibacterial, anticorrosion, the antioxidant addn of preparation makeup and articles for washing or as the purposes of foodstuff additive or as the purposes of healthcare products etc.
Claims (5)
1. one kind is utilized prominent holder wax plum essential oil that steam distillation extracts and uses thereof, it is characterized in that selecting for use prominent holder in autumn wax plum not have the complete fresh leaf of scab, gather, clean, natural air drying, pulverize, distillation, oily water separation can obtain prominent holder wax plum essential oil, oil yield is 0.7-1.5%, its main chemical compositions is (E)-3-(4,8-dimethyl-3,7-nonene base)-and furans 13.81%, acetyl norbornene ester 12.66%, 6,6-dimethyl-3-methylene radical-dicyclo [3.1.1] heptane 7.06%, tetrahydrochysene solasodine 5.93%, loudspeaker enol 5.32%, 1,5-dimethyl-1-vinyl-4 butyric acid hexene ester 5.09% composition such as grade accounts for 49.87% of total volatile oil composition; This essential oil has purposes widely in makeup, food and pharmaceutical industries.。
2. the preparation method of prominent holder wax plum essential oil according to claim 1 is characterized in that following these steps to carrying out:
(1) select the complete disease-free fresh blade of prominent holder wax plum in autumn for use, remove impurity, use flushing with clean water 1-3 time, natural air drying 12-48 hour, crushing screening 20-60 order, sealing is preserved standby;
(2) take by weighing a certain amount of prominent holder wax plum powder in round-bottomed flask, add water 5-10 and doubly measure, wet distillation 6-8 hour;
(3) distillate is loaded in the separating funnel, left standstill 0.5 hour, reclaim the faint yellow transparent oily liquid in upper strata;
(4) remove moisture with anhydrous sodium sulfate drying, be prominent holder wax plum essential oil, yield is: 0.7%-1.5%, and in brown air-tight bottle-20 ℃ preservation.
3. prominent holder wax plum essential oil according to claim 1 is antibacterial, anticorrosion as makeup and articles for washing, the purposes of antioxidant addn.
4. prominent holder wax plum essential oil according to claim 1 is as the purposes of preparation foodstuff additive.
5. prominent holder wax plum essential oil according to claim 1 is as the purposes of healthcare products.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105479090A CN102002439B (en) | 2010-11-17 | 2010-11-17 | Medicinal and edible plant chimonanthus grammatus essential oil, and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105479090A CN102002439B (en) | 2010-11-17 | 2010-11-17 | Medicinal and edible plant chimonanthus grammatus essential oil, and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102002439A true CN102002439A (en) | 2011-04-06 |
| CN102002439B CN102002439B (en) | 2013-11-06 |
Family
ID=43810142
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105479090A Expired - Fee Related CN102002439B (en) | 2010-11-17 | 2010-11-17 | Medicinal and edible plant chimonanthus grammatus essential oil, and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102002439B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031744A (en) * | 2014-06-27 | 2014-09-10 | 湖北五柳源生物科技有限责任公司 | Supercritical CO2 extraction method for chimonanthus praecox essential oil |
| CN106244325A (en) * | 2016-08-16 | 2016-12-21 | 重庆雅香美源生态农业科技有限公司 | The extraction of a kind of Herba Acroptili repentis quintessence oil and processing method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1557411A (en) * | 2004-01-17 | 2004-12-29 | 江西天海药业股份有限公司 | Soft capsule of wintersweet leaf and its preparing method |
| CN101254221A (en) * | 2007-07-20 | 2008-09-03 | 肖军平 | Preparation of Chimonanthus nitens Oliv. oral liquid for preventing and controlling cold |
-
2010
- 2010-11-17 CN CN2010105479090A patent/CN102002439B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1557411A (en) * | 2004-01-17 | 2004-12-29 | 江西天海药业股份有限公司 | Soft capsule of wintersweet leaf and its preparing method |
| CN101254221A (en) * | 2007-07-20 | 2008-09-03 | 肖军平 | Preparation of Chimonanthus nitens Oliv. oral liquid for preventing and controlling cold |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104031744A (en) * | 2014-06-27 | 2014-09-10 | 湖北五柳源生物科技有限责任公司 | Supercritical CO2 extraction method for chimonanthus praecox essential oil |
| CN106244325A (en) * | 2016-08-16 | 2016-12-21 | 重庆雅香美源生态农业科技有限公司 | The extraction of a kind of Herba Acroptili repentis quintessence oil and processing method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102002439B (en) | 2013-11-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Barbouchi et al. | A comparative study on phytochemical screening, quantification of phenolic contents and antioxidant properties of different solvent extracts from various parts of Pistacia lentiscus L | |
| Tsai et al. | Chemical composition and biological properties of essential oils of two mint species | |
| Li et al. | Antimicrobial properties, antioxidant activity and cytotoxicity of ethanol-soluble acidic components from Ganoderma atrum | |
| Xiong et al. | Screening and identification of the antibacterial bioactive compounds from Lonicera japonica Thunb. leaves | |
| Ebrahimabadi et al. | Essential oil composition and antioxidant and antimicrobial properties of the aerial parts of Salvia eremophila Boiss. from Iran | |
| Kim et al. | Rhus verniciflua Stokes flavonoid extracts have anti-oxidant, anti-microbial and α-glucosidase inhibitory effect | |
| Djouahri et al. | Effect of extraction method on chemical composition, antioxidant and anti-inflammatory activities of essential oil from the leaves of Algerian Tetraclinis articulata (Vahl) Masters | |
| Kulišić et al. | The effects of essential oils and aqueous tea infusions of oregano (Origanum vulgare L. spp. hirtum), thyme (Thymus vulgaris L.) and wild thyme (Thymus serpyllum L.) on the copper-induced oxidation of human low-density lipoproteins | |
| CN101697738B (en) | Method for continuously extracting antibactial substances and antioxidant substances from pyrolignucs acid | |
| Adeosun et al. | Essential oil from the stem bark of Cordia sebestena scavenges free radicals | |
| Dong et al. | Antioxidant activities and phenolics of fermented Bletilla formosana with eight plant pathogen fungi | |
| Sharma et al. | Evaluation of phytochemical and antioxidant activity of raw Pyrus communis (l), an underexploited fruit | |
| Zeragui et al. | Essential oil from Artemisia judaica L.(ssp. sahariensis) flowers as a natural cosmetic preservative: Chemical composition, and antioxidant and antibacterial activities | |
| Vardhini et al. | Antioxidant, anticancer, antibacterial activities and GCMS analysis of aqueous extract of pulps of Aegle marmelos (L.) Correa | |
| CN104327952A (en) | Pelargonium odoratissimum essential oil and extraction method and application of essential oil | |
| Prajna et al. | HPLC quantification of phenolic acids from Vetiveria zizanioides (L.) Nash and its antioxidant and antimicrobial activity | |
| CN102002439B (en) | Medicinal and edible plant chimonanthus grammatus essential oil, and preparation method and application thereof | |
| TIAN | Chemical constituents in essential oils from Elsholtzia ciliata and their antimicrobial activities | |
| Peerakam et al. | Chemical profiling revealed a dominant compound trans-anethole and biological evaluation of an edible plant Clausena harmandiana containing essential oil | |
| Derbel et al. | Chemical composition and biological potential of seed oil and leaf extracts of Henophyton deserti Coss. & Durieu | |
| Sukumaran et al. | General unknown screening, antioxidant and anti-inflammatory potential of Dendrobium macrostachyum Lindl. | |
| KR101717020B1 (en) | The composition comprising saponins derived from green tea seed having antifungal and anticancer activity and manufacturing method thereof | |
| CN107162891A (en) | A kind of naphthalene compounds extracted from lavender and its preparation method and application | |
| Li et al. | Comparative evaluation of the chemical composition of essential oil from twig, leaf and root of Clerodendrum inerme (L.) Gaertn | |
| CN113143994B (en) | Water extract of cinnamon oil production residue and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131106 Termination date: 20141117 |
|
| EXPY | Termination of patent right or utility model |