CN101993823B - Environmental self-control type cytology experimental platform - Google Patents
Environmental self-control type cytology experimental platform Download PDFInfo
- Publication number
- CN101993823B CN101993823B CN2009101696404A CN200910169640A CN101993823B CN 101993823 B CN101993823 B CN 101993823B CN 2009101696404 A CN2009101696404 A CN 2009101696404A CN 200910169640 A CN200910169640 A CN 200910169640A CN 101993823 B CN101993823 B CN 101993823B
- Authority
- CN
- China
- Prior art keywords
- cell culture
- semi
- cell cultures
- cytology
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a cytology experimental platform capable of working independently and automatically regulating a cell culture environment. The platform is characterized by comprising a cell culture environment self-regulating system, a quasi-open cell culture system, a multi-parameter monitoring system and a cell culture area, etc. The cell culture environment self-regulating system comprises a gas ingredient control system and a temperature control system. The quasi-open cell culture system is a cell culture system supported by a semi-permeable membrane technology and comprises a special cell culture bottle, a sample support, a semi-permeable membrane and a sealing cover and the like. The sample support is built in the cell culture bottle. The semi-permeable membrane is fixed at the inner side of the sealing cover. The multi-parameter monitoring system consists of a micro-processor, an A/D converter, a D/A converter, a parameter controller and a parameter sensor. The platform is remarkably characterized by having the ability to work independently, can be applied to simulation research on ground cytology microgravity effect, can automatically regulate the cell culture environment and ensure the normal growth of the cell.
Description
Technical field the present invention relates to a kind of cell experiment platform that is applied to research fields such as life science and cytobiology, particularly a kind of cell microgravity effect analog platform with the autonomous ability of regulation and control of cell culture environment.
Background technology receive the real-time experiment opportunity in aerospace medicine cytology space few, have high input and the restriction of factor such as risk height; The ground modeling effort just becomes the prerequisite and the important supplement of carrying out space-based research, and this pair cell is learned microgravity effect ground modeling effort enforcement platform and proposed more urgent demand.At present, the domestic and international research focus of ground analog platform development having become, the aerospace medicine cytology research ground modeling effort platform of particularly carrying out at cellular and molecular level has become the focus that this area is paid close attention to.
(RotateWall Vessels RWVs) is representative to the serial gyrator that the development of external microgravity effect ground analog platform is developed with NASA.This area has been developed the ground analog machine of the various models of different series at present and has been served different medical research targets.Just because of being widely used of aforesaid device, its limitation also progressively reveals.At first, existing installation is the regulating cell culture environment independently, can not satisfy medium-term and long-term aerospace medicine cytology research needs.Existing installation all adopts hermetically sealed cell cultures pattern, and the stable manual work through cell culture fluid of cell growing environment is changed and realized that this will influence the smooth implementation of follow-up longer time cytologic experiment.
The second, existing installation can not independent work, requires and the supporting use of auxiliary facility, can not be integrated in inferior magnetic machine and the man-made radiation's equipment, satisfied not the needs of the aerospace medicine cytology space compound effect study demanding urgently carrying out.
Summary of the invention the objective of the invention is to set up a kind of environment automatic control type cytologic experiment platform; Can be used for ground cytology microgravity effect analog research; And have independent work and cell culture environment auto-control ability, support the research of aerospace medicine cytology ground.Platform mainly comprises parts such as the autonomous regulator control system of cell culture environment, accurate open cell culture system, multiparameter supervisory system and cell cultures district.
The autonomous regulator control system of cell culture environment is the topworks that keeps the necessary growing environment of cell in the equipment, mainly comprises gas ingredients system and MCS.The target of described gas ingredients system is regulation and control CO
2Concentration is by gas cylinder, pressure tube, reducing valve, SV, strainer, CO
2Transmitter is formed, and all component parts connect through pressure tube successively.From the effusive CO of gas cylinder
2Gas is flowed through through pressure tube and is arrived SV behind the reducing valve.During the equipment requirements air feed, SV is opened, CO
2Gas is injection device cell cultures district after strainer filters, and the concentration rear electromagnetic valve closing device that meets the demands stops air feed automatically.Described temperature controlling system is made up of insulation construction, well heater, scatterer, blower fan and transmitter; Insulation construction is wrapped in the outside, cell cultures district; Well heater, scatterer and blower fan are fixed on one jiao in cell cultures district, and it is inboard that transmitter is installed on the cell cultures district.
Accurate open cell culture system is to serve as to support cell culture system with the semi-permeable membranes technology, comprises parts such as special-purpose Tissue Culture Flask, sample supporting rack, semi-permeable membranes and sealing cover.The sample supporting rack is built in Tissue Culture Flask, and semi-permeable membranes is fixed in the sealing cover inboard.
The function of multiparameter supervisory system is the watch-dog working status parameter, mainly comprises CO
2Concentration, cell cultures temperature and machine operation rotating speed etc.The multiparameter supervisory system is made up of microprocessor, A/D conversion, D/A conversion, parameter controller and parameter sensors.System passes through temperature, CO
2The size with set(ting)value is compared in the data gathering of concentration and rotating speed; Regulate the action of control power element, make actual value trend set(ting)value.
After the present invention adopted technique scheme, its distinguishing feature was:
1) have independent work capacity, works better does not rely on miscellaneous equipment;
2) but the auto-control cell culture environment guarantees the cell normal growth.
Description of drawings Fig. 1 is an one-piece construction synoptic diagram of the present invention.
The gas cylinder 2 that the gas ingredients system adopts among embodiment the present invention is the 300ml gas cylinder; Can tolerate 300 normal atmosphere; Reducing valve 1 connects with gas cylinder 2 through the M18 screw thread; Link with external diameter 5mm red copper pressure tube 3 between reducing valve 1 outlet and SV 4 inlet mouths, SV 4 effluent airs filter the back through 22 μ m strainers 5 and inject cell cultures district 6, CO
2Transmitter has selected 16 usefulness non-diffusion infrared sensor.
The medical polycarbonate injection moulded moulding of Tissue Culture Flask 7 usefulness in the open cell culture system of said standard, internal diameter 30mm, high 40mm, wall thickness 2mm, sample rack 8 can place 6 samples equally by medical polycarbonate injection moulded moulding.Semi-permeable membranes 9 is by medical grade silicon rubber processing, thick 0.1mm, sealing cover 10 connects with Tissue Culture Flask 7 through the M36 screw thread, tighten sealing cover 10 after, semi-permeable membranes 9 is pressed on Tissue Culture Flask 7 bottlenecks.
When equipment work, well heater 12 begins heating, and heat is transmitted to scatterer 13 backs and in cell cultures district 6, forms forced convection by blower fan 14, makes cell cultures district temperature maintenance at 36.5 ℃ ± 0.5 ℃; CO
2Gas flows out from gas cylinder 2, after reducing valve 1 decompression, arrives SV 4, when cell cultures district 6 requires to replenish CO
2The time, multiparameter supervisory system control SV 4 is opened CO
2Gas stream is crossed SV 4 and after strainer 5 filters, is injected cell cultures district 6, guarantees cell cultures district CO
2Concentration maintains 5% ± 0.3% level.CO
2Get in the Tissue Culture Flask 7 through semi-permeable membranes 9, satisfy cell growth requirement.
After such scheme was realized, equipment had had capacity of working on one's own, and ability regulates cell cultures district environment automatically, satisfied the requirement of cell normal growth.
Claims (1)
1. environment automatic control type cytologic experiment platform; Comprise the autonomous regulator control system of cell culture environment, accurate open cell culture system, multiparameter supervisory system and cell cultures district, it is characterized in that: the autonomous regulator control system of described cell culture environment comprises gas ingredients system and temperature controlling system; The gas ingredients system is made up of gas cylinder, pressure tube, reducing valve, SV, strainer, CO2 transmitter, and all component parts connect through pressure tube successively; Temperature controlling system is made up of insulation construction, well heater, scatterer, blower fan and transmitter, and insulation construction is wrapped in the outside, cell cultures district, and well heater, scatterer and blower fan are fixed on one jiao in cell cultures district, and it is inboard that transmitter is installed on the cell cultures district; The open cell culture system of described standard is to serve as to support cell culture system with the semi-permeable membranes technology, comprises special-purpose Tissue Culture Flask, sample supporting rack, semi-permeable membranes and sealing cover, and the sample supporting rack is built in Tissue Culture Flask, and semi-permeable membranes is fixed in the sealing cover inboard; The function of described multiparameter supervisory system is the watch-dog working status parameter, is made up of microprocessor, A/D conversion, D/A conversion, parameter controller and parameter sensors.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101696404A CN101993823B (en) | 2009-08-28 | 2009-08-28 | Environmental self-control type cytology experimental platform |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101696404A CN101993823B (en) | 2009-08-28 | 2009-08-28 | Environmental self-control type cytology experimental platform |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101993823A CN101993823A (en) | 2011-03-30 |
| CN101993823B true CN101993823B (en) | 2012-11-07 |
Family
ID=43784579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009101696404A Active CN101993823B (en) | 2009-08-28 | 2009-08-28 | Environmental self-control type cytology experimental platform |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101993823B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103667053B (en) * | 2013-05-17 | 2015-07-29 | 中国航天员科研训练中心 | Automatic continuous changes liquid cell culture apparatus |
| CN105954505B (en) * | 2016-05-04 | 2018-06-15 | 浙江大学 | The detection device and method of the diarrhoeal toxin of shellfish based on cell activity sensor |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1235634A (en) * | 1996-11-01 | 1999-11-17 | 基因观测公司 | Cell culture incubator |
| CN1521253A (en) * | 2003-01-27 | 2004-08-18 | 英科新创(厦门)科技有限公司 | Cell culture incubator |
| CN1737106A (en) * | 2000-03-02 | 2006-02-22 | 高木产业株式会社 | Method of and apparatus for cultivating a cell or tissue |
-
2009
- 2009-08-28 CN CN2009101696404A patent/CN101993823B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1235634A (en) * | 1996-11-01 | 1999-11-17 | 基因观测公司 | Cell culture incubator |
| CN1737106A (en) * | 2000-03-02 | 2006-02-22 | 高木产业株式会社 | Method of and apparatus for cultivating a cell or tissue |
| CN1521253A (en) * | 2003-01-27 | 2004-08-18 | 英科新创(厦门)科技有限公司 | Cell culture incubator |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101993823A (en) | 2011-03-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106916726B (en) | A kind of fermentation process coupled with separation system for continuously fermenting on a large scale | |
| CN101993823B (en) | Environmental self-control type cytology experimental platform | |
| CN202506339U (en) | Cooling device for coating production | |
| CN103667053B (en) | Automatic continuous changes liquid cell culture apparatus | |
| CN203923199U (en) | A kind of ferment at constant temperature tank | |
| GB201118425D0 (en) | Bioreactor outlet air conditioning systems and associated methods | |
| CN203190610U (en) | Heat preservation container device for water machine | |
| CN202048371U (en) | Fluid Emptying utilization device in fluid supply system or equipment and system or equipment containing same | |
| CN203498903U (en) | Intelligent constant pressure water supply device | |
| CN205653169U (en) | Incubator | |
| CN108998372A (en) | A kind of microbial immobilized sphere preparation facilities | |
| CN202322338U (en) | Novel constant-temperature separating funnel | |
| CN2846096Y (en) | Film making device | |
| CN206624718U (en) | Instant heating type water purifier | |
| CN102671249B (en) | A kind of blood purification temperature controls and off gas system | |
| CN201059706Y (en) | Device for automatically regulating air-conditioning house humidity | |
| CN201470325U (en) | Membrane separating cup | |
| CN204513795U (en) | A kind of solar water heater intelligence control system | |
| CN101118080B (en) | Central air-conditioning terminal intelligent dynamic constant-current control device | |
| CN204529875U (en) | The automatic culturing and adding device of a kind of microbial bacteria | |
| CN203038134U (en) | Cold and hot liquid temperature and flow constant output intelligence control system | |
| CN206970579U (en) | PLC controls microbiological incubator | |
| CN218554127U (en) | Constant temperature and humidity incubator | |
| CN220393715U (en) | Agitating unit and fusion protein biological fermentation jar | |
| CN216347288U (en) | Automatic constant-temperature anti-condensation temperature control device in clean room system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |