CN101975746A - Method for determining rate constant for micro-biological degradation of restaurant oil smoke pollutants - Google Patents

Abstract

The invention discloses a method for determining a rate constant for micro-biological degradation of restaurant oil smoke pollutants, which comprises the following steps: putting FeCl3 and H2O in a beaker, sequentially adding KH2PO4, K2HPO4, MgSO4, CaCl2, MnSO4 and NH4NO3, stirring until the substances are dissolved, and preparing into a nutrient solution; selecting 10 L of supernate of active sludge from a municipal domestic sewage treatment plant, adding 25mL of the nutrient solution into the supernate, continuously aerating, intermittently introducing oil smoke gas, and culturing for 21d; respectively containing 500mL of the culture solution in two conical flasks of 1000mL, plugging the flasks, culturing in a shaking table with a constant-temperature water bath for 6d, sampling 25mL of water every 2h, extracting the water sample with 25mL of carbon tetrachloride, and selecting 15mL of oil phase to respectively determine the concentration of oil smoke pollutants in the sample; and making a degradation rate curve by using the ratio of the liquid phase oil concentration difference to the time interval as the longitudinal coordinate and the liquid phase oil concentration as the horizontal coordinate, and fitting a linear regression equation, wherein the slope of the regression equation is the primary kinetic rate constant for the micro-biological degradation of restaurant oil smoke pollutants. The invention has the advantages of simple and practicable determining method, low cost and high accuracy.

Description

A kind of definite method of microbial degradation rate constant of food and drink pollutants from cooking fume

Technical field

The present invention relates to a kind of definite method of microbial degradation rate constant of food and drink pollutants from cooking fume.

Technical background

Microorganism is the biochemical reaction process of a complexity to the degradation process of single organic compound, in course of reaction microorganism at first the secretory cell exoenzyme insoluble larger molecular organics is resolved into the solubility small organic molecule, passing cell membrane then enters in the cell, under various desmoenzyme effects, or be utilized synthetic new cell, or oxidized, for the vital movement of bacterium provides energy.Because these reactions all are to carry out under the effect of biology enzyme, therefore be called again " enzymatic reaction ".

Oil smoke is the condensation product of the oil flue waste gas thing that produces in the food high temperature process process, contains a large amount of with multiple compositions such as the solid-state or liquid grease that exists, fatty acid, glycerine, food proteins.Must rely on the division of labor of mixed microorganism group, synergy to finish for this biodegradation that comprises the target contaminant of multiple compound.Biodegradation to different compounds in the oil smoke, microorganism need be finished by a series of intermediate reaction, and connect each other between these reactions, cooperatively interact with cooperative mode is to lean on the regulatory function of microorganism to realize, and the domestication of microorganism is to make microorganism have the important means of this regulatory function.

Relation between research growth of microorganism and the target contaminant degradation rate and the knowledge of each factor affecting be called Biodegradation Kinetics.Be used to study the dynamic (dynamical) model of microbial degradation at present and mainly contain 2 kinds on index speed model and hyperbolic velocity model, the index speed model is only applicable to the biochemical reaction of homogeneous phase solution; And the hyperbolic velocity model is adapted to pass through surface adsorption or surface and the compound enzymic catalytic reaction that carries out of catalytic molecular.

Summary of the invention

The objective of the invention is to determine a kind of assay method of liquid pollutants from cooking fume microbial degradation rate constant, for biological method purification food and drink oil smoke provides basic data.

The present invention is on the basis that microorganism fungus kind has carried out through abundant domestication, environment and nutritional condition optimizing, the physicochemical property of used liquid oil smoke also are under the invariable condition of strict maintenance, therefore the degradation rate problem of the liquid pollutants from cooking fume of research microbial degradation is suitable for the index speed model.

Its expression formula :-dc/dt=Kc ⁿ

In the formula: C-pollutant levels mg/L;

The t-degradation reaction time, h;

The K-velocity constant;

The n-order of reaction is more than or equal to 1.

Degradation rate constant characterizes the degradation capability of microorganism to specific pollutants, is to judge one of important parameter that can pollutant carry out a biological disposal upon.

Concrete steps of the present invention are:

1. activated sludge acclimatization: take by weighing 9～11g FeCl respectively ₃With 500mL H ₂O places the 1000mL beaker, and order adds 47～52g KH ₂PO ₄, 47～52g K ₂HPO ₄, 19～21g MgSO ₄, 9～11g CaCl ₂, 0.09～0.11g MnSO ₄With 9～11g NH ₄NO ₃, stir and to treat that being settled to 1000mL after its dissolving is mixed with nutrient solution, get the active sludge supernatant 10L of city domestic sewage treatment plant, to wherein adding the above-mentioned nutrient solution of 25mL, and continuous aeration, be interrupted feeding soot gas, the 1st week, every day 2h, in afternoon in the morning each 1h; The 2nd week, every day 3h, in afternoon in the morning each 1.5h; The 3rd week, every day 4h, in afternoon in the morning each 2h; Cultivate 21d;

2. shaking table test: respectively get above-mentioned nutrient solution 500mL with 2 1000mL conical flasks, live bottleneck with the medical drugs tampon, put into the shaking table of band water bath with thermostatic control (30 ℃ of biochemical temperature) and cultivate 6d, every 2h water sampling 25mL, measure 25mL carbon tetrachloride extraction water sample, get oil phase 15mL and determine fume pollution substrate concentration in the sample respectively with Infrared Oil Determination Instrument;

3. one-level degradation kinetics rate constant is definite: according to exponential model :-dc/dt=Kc ⁿ,

N gets 1 (one-level); Exponential model can be changed into: C _m-C _M-1/ (t _m-t _M-1)=KC _m，

In the formula: m-is a sample number;

The C-pollutant levels, mg/L;

The t-degradation reaction time, h.

Ratio with the liquid phase oil content concentration difference and the time interval is ordinate, is that horizontal ordinate is made the degradation rate curve with liquid phase oil content concentration, and the match equation of linear regression is got the microbial degradation first order kinetics rate constant that its slope is the food and drink pollutants from cooking fume.

Advantage of the present invention is: assay method is simple, and is with low cost, and accuracy is good.

Embodiment

Embodiment:

1. activated sludge acclimatization: take by weighing 10g FeCl respectively ₃With 500mL H ₂O places the 1000mL beaker, and order adds 50g KH ₂PO ₄, 50g K ₂HPO ₄, 20g MgSO ₄, 10g CaCl ₂, 0.1g MnSO ₄With 10g NH ₄NO ₃, stir and treat that being settled to 1000mL after it dissolves is mixed with nutrient solution, gets the active sludge supernatant 10L of city domestic sewage treatment plant with a 25L Plastic Drum, to wherein adding the above-mentioned nutrient solution of 25mL, and continuous aeration, be interrupted feeding soot gas, the 1st week, every day 2h, in afternoon in the morning each 1h; The 2nd week, every day 3h, in afternoon in the morning each 1.5h; The 3rd week, every day 4h, in afternoon in the morning each 2h; Cultivate 21d;

2. shaking table test: respectively get above-mentioned nutrient solution 500mL with 2 1000mL conical flasks, live bottleneck with the medical drugs tampon, put into the shaking table of band water bath with thermostatic control (30 ℃ of biochemical temperature) and cultivate 6d, every 2h water sampling 25mL, measure 25mL phenixin (environmental protection special use) extraction water sample, get oil phase 15mL and determine fume pollution substrate concentration in the sample respectively with Infrared Oil Determination Instrument;

3. one-level degradation kinetics rate constant is definite: according to exponential model :-dc/dt=Kc ⁿ,

N gets 1 (one-level); Exponential model can be changed into: C _m-C _M-1/ (t _m-t _M-1) KC _m，

In the formula: C-pollutant levels mg/L;

The t-degradation reaction time, h;

The K-velocity constant;

The n-order of reaction equals 1;

M-is a sample number.

Ratio with the liquid phase oil content concentration difference and the time interval is ordinate, is that horizontal ordinate is made the degradation rate curve with liquid phase oil content concentration, and the match equation of linear regression is got the microbial degradation first order kinetics rate constant that its slope is the food and drink pollutants from cooking fume.

Claims (1)

1. definite method of the microbial degradation rate constant of a food and drink pollutants from cooking fume is characterized in that concrete steps are:

(1) activated sludge acclimatization: take by weighing 9～11g FeCl respectively ₃With 500mL H ₂O places the 1000mL beaker, and order adds 47～52g KH ₂PO ₄, 47～52g K ₂HPO ₄, 19～21g MgSO ₄, 9～11g CaCl ₂, 0.09～0.11g MnSO ₄With 9～11g NH ₄NO ₃, stir and to treat that being settled to 1000mL after its dissolving is mixed with nutrient solution, get the active sludge supernatant 10L of city domestic sewage treatment plant, to wherein adding the above-mentioned nutrient solution of 25mL, and continuous aeration, be interrupted feeding soot gas, the 1st week, every day 2h, in afternoon in the morning each 1h; The 2nd week, every day 3h, in afternoon in the morning each 1.5h; The 3rd week, every day 4h, in afternoon in the morning each 2h; Cultivate 21d;

(2) shaking table test: respectively get above-mentioned nutrient solution 500mL with 2 1000mL conical flasks, live bottleneck with the medical drugs tampon, put into the shaking table of band water bath with thermostatic control and cultivate 6d, 30 ℃ of biochemical temperature, every 2h water sampling 25mL, measure 25mL carbon tetrachloride extraction water sample, get oil phase 15mL and determine fume pollution substrate concentration in the sample respectively with Infrared Oil Determination Instrument;

(3) determining of one-level degradation kinetics rate constant: according to exponential model :-dc/dt=Kc ⁿ,

.n get 1; Exponential model can be changed into: C _m-C _M-1/ (t _m-t _M-1)=KC _m，

In the formula: C-pollutant levels, mg/L;

The t-degradation reaction time, h;

The K-velocity constant;

The n-order of reaction is more than or equal to 1;

M-is a sample number;

Ratio with the liquid phase oil content concentration difference and the time interval is ordinate, is that horizontal ordinate is made the degradation rate curve with liquid phase oil content concentration, and the match equation of linear regression is got the microbial degradation first order kinetics rate constant that its slope is the food and drink pollutants from cooking fume.