CN101974382A - Process for extracting beer waste yeast fermentation enzymatic hydrolysate - Google Patents

Abstract

The invention discloses a process for extracting beer waste yeast fermentation enzymatic hydrolysate, which comprises the following steps of: filtering the beer waste yeast fermentation enzymatic hydrolysate by using a metallic film or a ceramic film; putting the yeast extract clear solution after filtering into subsequent processing process steps, returning part of mother liquid to a circulating tank, and performing cycling filtration on the other part of the mother liquid after processing the other part of the mother liquid in a circulating pump; when the percentage of solid substances in the enzymatic hydrolysate reaches 25 to 35 percent, feeding deionized water to perform dialysis; and when the content of yeast extracts in the mother liquid is less than 100 mg/L to 300 mg/L, finishing the extraction process. The process can effectively extract the yeast extract clear solution in the enzymatic hydrolysate and has high separation speed and high accuracy, the yeast extract clear solution after separation has high clarity, and the yield reaches up to 97 and 98 percent. The process avoids several times of separation, and shortens the production cycle, and the separation time of 20 tons of the beer waste yeast fermentation enzymatic hydrolysate can be shortened by 12 to 15 hours. Furthermore, the process can ensure that beer waste yeasts are fully used, can be used for producing high-value products and changing waste into valuable, and can realize zero pollution and zero release, thereby realizing clean production and greatly reducing the environmental protection pressure.

Description

A kind of beer waste yeast fermentation enzymolysis solution extraction process

Technical field

The present invention relates to the utilization again of beer waste yeast, particularly beer waste yeast fermentation enzymolysis solution extraction process.

Background technology

China's beer production has reached 4,000 ten thousand tons, genuine beer production and consumption big country have been become, increase year after year along with China's beer production, the also corresponding increase of the quantity discharged of beer waste yeast, data shows according to statistics, 1000 tons of beer of every production will produce the waste yeast of 1.5 tons of about dry weights, and therefore, the problem of complex utilization of beer waste yeast has caused showing great attention to of government and relevant enterprise.

At present, the domestic enterprise that has is developed to yeast extract with beer waste yeast, but owing to only adopted self-dissolving technology, and leaching process is generally centrifugation, clear liquid clarity after the centrifugation is poor, its extract yield and quality, Yeast Nucleic Acid percent hydrolysis and quality are all lower, the whole weak flavor of finished product yeast extract; And repeatedly centrifugal, the production cycle is long; The centrifugation cycles of concentration is low, and the washing amount is big, causes follow-up clear liquid concentration and evaporation energy consumption higher, and the residue after the extracting still makes refuse and handle, and precious resources such as wherein contained mannooligo saccharide and beta-glucan fail to be utilized effectively; Compared with developed countries, China is on also remaining at low levels aspect the comprehensive utilization of beer waste yeast.

Therefore yeast extract fully discharges in the beer waste yeast in order to make, the yield and the quality of yeast extract in the beer waste yeast fermentation enzymolysis solution are improved, and making cell walls behind the waste yeast broken wall make the product of high value, the utilization again of beer waste yeast seems particularly important.

Summary of the invention

The object of the invention provides a kind of beer waste yeast fermentation enzymolysis solution extraction process, improving the quality and the yield of yeast extract, and makes cell walls behind the waste yeast broken wall make the product of high value, realizes the waste yeast zero release.

For realizing the object of the invention, this beer waste yeast fermentation enzymolysis solution extraction process is characterized in that may further comprise the steps:

A. beer waste yeast fermentation enzymolysis solution is after injecting circulation tank under 55～65 ℃ of temperature, is metallic membrane or the ceramic membrane filter of 0.45 μ m～20nm by the aperture under 55～65 ℃, 0.2～0.5Mpa condition;

B. the yeast extract clear liquid after the above-mentioned filtering separation enters the following process operation, and a mother liquor part is returned circulation tank, and another part passes through metallic membrane or ceramic membrane circulating filtration behind recycle pump;

C. when the solid matter weight percent reached 25～35% in the beer waste yeast fermentation enzymolysis solution, stream added the deionized water dialysis in circulation tank, and when yeast extract content in the mother liquor during less than 100mg/L～300mg/L, leaching process finishes.

The amount that described stream adds deionized water is 0.5～0.6 times of mother liquor amount.

The technical progress that the present invention obtains:

(1). owing to adopt technology of the present invention that yeast extract in the beer waste yeast fermentation enzymolysis solution is fully discharged, the mode that adopts stream the to add water yeast extract in the beer waste yeast enzymolysis solution of dialysing, can extract yeast extract clear liquid in the beer waste yeast fermentation enzymolysis solution effectively, velocity of separation is fast, the separation accuracy height, yeast extract clear liquid clarity height after the separation, the quality height of the Yeast Nucleic Acid that finally obtains, yeast extract, and yield is up to 97% and 98%.

(2). owing to adopt technology of the present invention, the waste yeast mother liquor after extract extracts in the beer yeast fermenting enzymolysis solution can obtain beta-glucan, mannooligo saccharide through subsequent disposal, and yield is all up to more than 97%.

(3). adopt technology of the present invention, avoided the separating for several times of whizzer, shortened the production cycle, 20 tons beer waste yeast enzymolysis solution can shorten 12～15 hours disengaging time.Beer waste yeast is fully utilized, can produces the product of high value, turn waste into wealth, and can realize zero pollution, zero release, realize clean production, reduce the environmental protection pressure of brew-house greatly, have remarkable economic efficiency and social benefit.

Description of drawings

Fig. 1 is a process flow diagram of the present invention.

Embodiment

As shown in Figure 1, at first beer waste yeast is fermented enzymolysis solution after injecting beer waste yeast fermentation enzymolysis solution circulation tank 1 under 55～65 ℃ the temperature, be incubated at 55～65 ℃ through interchanger 3, tensimeter 5 is to be that the metallic membrane 6 of 0.45 μ m～20nm carries out filtering separation by the aperture under the condition of 0.2～0.5Mpa, yeast extract clear liquid after the separation is sent into and is entered subsequent handling processing in the clear liquid storage tank 7 again, a beer waste yeast fermentation enzymolysis solution mother liquor part turns back in the beer waste yeast fermentation enzymolysis solution circulation tank 1, another part is at valve 8, circulating filtration in the internal circulation system that recycle pump 4 and metallic membrane 6 form, round-robin simultaneously, under the effect of pressure, there is part yeast extract clear liquid to ooze out, beer waste yeast fermentation enzymolysis solution in the enzymolysis solution of the beer waste yeast fermentation simultaneously circulation tank 1 is constantly sent in the internal recycle by pump 2, the amount of the beer waste yeast fermentation enzymolysis solution of sending into by pump 2 be yeast extract clear liquid seepage discharge with turn back to amount in the beer waste yeast fermentation enzymolysis solution circulation tank 1 and, solid matter weight percent until beer waste yeast fermentation enzymolysis solution reaches at 25～35% o'clock, beginning stream in beer waste yeast fermentation enzymolysis solution circulation tank 1 adds deionization dialysis water, the stream dosage of deionization dialysis water is 0.5～0.6 times of mother liquor amount, after stream adds the water end, when yeast extract content is less than 100mg/L～300mg/L in the beer waste yeast fermentation enzymolysis solution mother liquor, leaching process finishes, and beer waste yeast fermented liquid mother liquor directly enters subsequent technique from 1 discharge of beer waste yeast fermentation enzymolysis solution circulation tank and prepares beta-glucan, mannooligo saccharide.

Working process of the present invention is:

Beer waste yeast is fermented enzymolysis solution under 55～65 ℃ temperature, send into the metallic membrane cross flow filter internal recycle from beer waste yeast fermentation enzymolysis solution circulation tank 1 and to extract, in this process, under the effect of pressure, constantly see through metallic membrane less than the material of membrane pore size in the beer waste yeast fermentation enzymolysis solution and ooze out and become high-quality yeast extract and enter subsequent handling.The beer waste yeast fermentation enzymolysis solution mother liquor of being held back by metallic membrane continuously turns back in the beer waste yeast fermentation enzymolysis solution circulation tank 1 simultaneously, enters internal circulation system and carry out circulating filtration after pump 2 is sent into interchanger 3 insulations.When the solid matter weight percent reaches 25～35% in the beer waste yeast fermentation enzymolysis solution mother liquor, beginning stream in beer waste yeast fermentation enzymolysis solution circulation tank 1 adds deionization dialysis water, after stream adds the water end, substantially do not have yeast extract in the beer waste yeast fermentation enzymolysis solution mother liquor, leaching process finishes.Beer yeast fermenting liquid mother liquor is discharged from beer waste yeast fermentation enzymolysis solution circulation tank 1 and is entered subsequent technique, makes beta-glucan, mannooligo saccharide.

Separate in order to continue next time, the metallic membrane after the use needs water or clean-out system to carry out the recovery regeneration of flux, prevents that effectively film from polluting.

From above process as can be known, the present invention can make the mode of beer waste yeast by the enzymolysis that ferments fully discharge wherein yeast extract, yeast extract can make the Yeast Nucleic Acid and the yeast extract of high nutritive value, yeast cells wall also can make beta-glucan, mannooligo saccharide, and beer waste yeast is utilized fully again.

Embodiment 2: the present embodiment difference from Example 1 is that metallic membrane is changed into ceramic membrane.

Claims (2)

1. beer waste yeast fermentation enzymolysis solution extraction process is characterized in that may further comprise the steps:

A. beer waste yeast fermentation enzymolysis solution is after injecting circulation tank under 55～65 ℃ of temperature, is metallic membrane or the ceramic membrane filter of 0.45 μ m～20nm by the aperture under 55～65 ℃, 0.2～0.5Mpa condition;

B. the yeast extract clear liquid after the above-mentioned filtering separation enters the following process operation, and a mother liquor part is returned circulation tank, and another part passes through metallic membrane or ceramic membrane circulating filtration behind recycle pump;

C. when the solid matter weight percent reached 25～35% in the beer waste yeast fermentation enzymolysis solution, stream added the deionized water dialysis in circulation tank, and when yeast extract content in the mother liquor during less than 100mg/L～300mg/L, leaching process finishes.

2. beer waste yeast according to claim 1 fermentation enzymolysis solution extraction process is characterized in that amount that described stream adds deionized water is 0.5～0.6 times of mother liquor amount.

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