CN101971925A - Application of lactulose and glutamine for replacing feeding antibiotics materials in broiler daily ration - Google Patents
Application of lactulose and glutamine for replacing feeding antibiotics materials in broiler daily ration Download PDFInfo
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- CN101971925A CN101971925A CN2010102940795A CN201010294079A CN101971925A CN 101971925 A CN101971925 A CN 101971925A CN 2010102940795 A CN2010102940795 A CN 2010102940795A CN 201010294079 A CN201010294079 A CN 201010294079A CN 101971925 A CN101971925 A CN 101971925A
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Abstract
The invention discloses an application of lactulose and glutamine for replacing feeding antibiotics materials in broiler daily ration, belonging to the technical field of broiler cultivation, in particular to an application of lactulose and compatibility of lactulose and glutamine for replacing feeding antibiotics materials in broiler daily ration. According to the invention, the lactulose and the compatibility of lactulose and glutamine are added in the broiler daily ration at a certain ratio, thus be capable of completely replacing various feeding antibiotics used by the broiler daily ration, and having the same actions on ensuring broiler to be healthy, accelerating broiler growth and improving feed conversion efficiency of the feed as the feeding antibiotics. The invention can be used for broilers without any feeding antibiotics and used for producing the products thereof.
Description
Technical field
The invention belongs to fryer cultural technique field, particularly can replace the lactulose of feeding antibiotic in the daily ration of broiler, the application of glutamine.
Background technology
In poultry production, use somatotrophic feed addictive can not only improve the immune response of poultry, and can increase the drug resistance of pathogen.Although many antibiotics growth promoters are having undeniable effect aspect the raising animal productivity and the price of deed,, antibiotic is subjected to extensive concern owing to can causing the raising of human pathogen drug resistance.Therefore, press for and find the feed addictive that not only can be used as healthy poultry production use, and the product that can when the barn-door fowl disease prevention and cure, use.Seeking a kind of antibiotic substitute improves the animal productivity and guarantees that the livestock products quality safety receives much concern.At present, scientists has been developed the non-antibiotic application strategy that can improve animal health, productivity and food security in a large number, but owing to the fryer feeding antibiotic substitute of using at present exists that additive capacity is higher, active ingredient is single, can not substitute reasons such as feeding antibiotic, effect in the daily ration of broiler be uncertain fully, therefore, the new fryer feeding antibiotic substitute of development just seems very urgent and important.
Lactulose has another name called milk ketose, Duphalac, is the derivative of lactose isomery, by galactolipin and the fructose disaccharides with the combination of β-1,4 glycosidic bond.The lactulose finished product is faint yellow clear and bright sticky body, odorless, and it is sweet to distinguish the flavor of.Its crystalline solid is white irregular powder, and is soluble in water, and viscosity is low, calorific value is low, safe, good stability, Maillard reaction does not take place.
Studies show that, lactulose is not absorbed in the nonruminant small intestine, can decompose the carbon source that becomes bifidobacterium growth for the beta galactosidase that Bifidobacterium produces after entering colon, thereby promote Bifidobacterium propagation, adjustment gut flora balance (Shi Shushi, Wang Liqun, Qiao Jianguo. feed the experiment [J] of Bifidobacterium propagation in the stool in mice with milk ketose milk powder. health research, 1993,22 (4): 238-239.).(Feilim J such as Feilim, Maxwell, Sylvia H, et al.Isolation, growth on prebioticsand probiotic potential of novel bifidobacteria from pigs[J] .Anaerobe, 2004,10 (1): 33-39.) oligosaccharide co-incubation such as the Bifidobacterium that will extract from the pig manure of all ages and classes section and lactulose, the culture medium Bifidobacterium breeding that wherein contains lactulose is best.Hoffmann (Hoffmann K.Behandlung von gesunden Salmonellen-Ausscheidern mitLactulose[J] .Dtsch Med Wochenschr, 1975,100:1429-1431.) report, lactulose can combine with salmonella as carrier and suppress its survival in enteron aisle.Lactulose stimulates the propagation of beneficial bacterium and the absorption of nutritional labeling, suppresses pathogen breeding and field planting, reduces the effect of the factors such as phytolectin of environment in being unfavorable for; To immune factor have direct stimulation or protective effect (Lee main forces. the function of compound sugar and to the influence [D] of weanling pig production performance and non-specific immune function. Harbin: Northeast Agricultural University, 2003.).Lactulose can influence animal to sugar, fat, trace elements absorbed and metabolism.(Suzuki K such as Suzuki, Vehara M, Endo Y, et al.Effect of lactose, lactulose andsorbitol on iron, zinc and copper utilization in rats[J] .Nippon EvoShokuryo Gakkaishi, 1986,39:217-219.) with being rich in lactose, lactulose, the food of sorbierite and the starch mouse 46d that feeds, found that, Ca, Mg, Cu, Fe, the absorptivity of Zn and RT in vivo all are higher than control group, caecum pH value step-down, and caecum weight and Bifidobacterium content are all greater than control group.(Robert Brommage such as Robert, Claodine Binacoa, Simone antille, etal.Intestinal Calcium Absorption in Rats Is Stimulated by DietaryLactulose and Other Resistant Sugars[J] .The Journal of Nutrition, 1993,123 (12): 2186.) studied the influence that lactulose absorbs enteron aisle Ca, found that, lactulose is stronger than lactose to the absorption facilitation of Ca, especially to 5～38 the week ages mouse.(GeeverE F such as Geever, Kan D, Levenson S M.Effect of bacterial flora on iron absorptionin rat[J] .Gastroenterol, 1968,55:690-694.) report, germfree mouse is significantly higher than common mouse to absorbing of iron.Lactulose has the discharge that increases neutral sterol, reduce the absorption of cholesterol, reduce the generation of secondary bile acid and other toxin, effect (the Rotstein O D of protection butyrate, Kay RM, Wayman M.Prevention of cholesterol gallstones by lignin and lactulosein the hamster[J] .Gastroenterology, 1981,81:1098-1103.; Van der, WerfS D J, Ruben A T.Effect of lactulose on biliary lipid composition[J] .JHepatology, 1986,3:328-332.).In addition, can reduce enteron aisle pH, suppress to produce endotoxic harmful bacterium breeding, and minimizing toxin source and absorption (Magijulo E.Endotoxin spillover on vitalhepatitis[J] .Infection, 1979,7:155; Wang Yongli, study of the Chinese classic source. rabbit gavages lactulose prevention and treatment endotoxemia [J]. Norman Bethune Medical University's journal, 1991,17 (4): 339-340.).
Glutamine is the main energy substrate of intestinal epithelial cell metabolism, also is the essential nutrients of lymphoid tissue propagation between enteric epithelium.The biological agent key of glutamine in alimentary canal is the stage of sucking the breast in early days, weaning stage.Participated in the intestinal tract injury repair process at these two stage glutamine.Glutamine is the protective effect mechanism of newborn piglet enteron aisle: the enterocyte of newborn piglet can be by the synthetic arginine of glutamine; be early stage anabolism (the Boon L that plays an important role; Meijer A J.Cont rol by p Hof urea synthesis in isolated rat hepatocytes[J] .Eur J Biochem; 1988,172:4652469.).In the weanling pig daily ration, add glutamine and can improve blood plasma glutamine concentration, height of naps, the crypts degree of depth, pit cell growth rate and mucosa DNA, protein content and glutaminase active.Open the army and the people and wait (the army and the people, Gao Zhenchuan. glutamine is to the influence [J] of early-weaned piglets intestine membrane protein matter, dna content and tissue morphology. Scientia Agricultura Sinica, 2002,35 (10): discovering 126421268.): in the weanling pig daily ration, add glutamine and can obviously improve growing of jejunum stage casing intestinal villus, keep the normal configuration and the function of small intestine.Dai Sifa etc. (golden light is bright for Dai Sifa, Wang Like, Liu Weiguo, Wang Jue, Li Shenghe. and glutamine is to AA chicken early growth Effect on Performance [J]. the journal .2002 of Anhui Technology Normal College; 16:1-3) studies show that, in the chicken feed, add G1n, also can significantly improve meat the chick growth performance and the daily ingestion amount in early stage (0～4 age in week).
Still not having at present the research data or the data of feeding antibiotic in the alternative daily ration of broiler of relevant lactulose, lactulose and glutamine compatibility openly reported.The invention provides the application of lactulose, lactulose and glutamine compatibility as feeding antibiotic substitute matter in the daily ration of broiler.
Summary of the invention
The object of the present invention is to provide the usage that substitutes feeding antibiotic in the daily ration of broiler with lactulose, lactulose and glutamine compatibility fully.In daily ration of broiler, add lactulose, glutamine by a certain percentage, not only can substitute employed various feeding antibiotics in the daily ration of broiler prescription fully, and have the effect that can guarantee fryer health, promotion growth of meat chicken, raising feed efficiency that feeding antibiotic has.The present invention can be used for not adding the production of fryer of any feeding antibiotic and products thereof.
The object of the present invention is achieved like this: (1) addition manner in the basal diet of three yellow chicken different growing stages is: add 5 grams in every kilogram of basal diet to 15 gram lactuloses, mix; (2) addition manner is in the basal diet of white plumage fryer different growing stages: fryer 0 to 21 day, add 1 gram glutamine in every kilogram of basal diet, and fryer 22 days to 42 days adds 10 gram lactuloses in every kilogram of basal diet, mix; Or fryer 0 to 42 day, add 1 gram glutamine, 10 gram lactuloses in every kilogram of basal diet, mix; Or fryer 0 to 42 day, add 10 gram lactuloses in every kilogram of basal diet, mix.
Lactulose of the present invention is to be produced by the rich industry and trade Co., Ltd of Shanghai card.
The optimum formula of basal diet of the present invention (powder) is formed in following ratio:
Yellow corn powder 61% big dregs of beans 30% fish meal 2% wheat bran 1.9% stone flour 1.3%
1.0% premix 1.0% of calcium monohydrogen phosphate 1.5% salt 0.3% soya-bean oil.
Lactulose of the present invention can stimulate the absorption of the propagation and the nutritional labeling of beneficial bacterium, suppresses pathogen breeding and field planting, strengthens the anti-infectious immunity ability; Reduce the effect of the factors such as phytolectin of environment in being unfavorable for, evenly add in the daily ration of broiler and can regulate fryer intestinal microecology balance behind the alternative feeding antibiotic, reduce owing to the immunological stress state that infringement caused of harmful microorganism fryer, guarantee fryer health, reduce death rate, suitable with the effect of adding feeding antibiotic on daily gain, material anharmonic ratio, therefore, use usage of the present invention and can substitute the various feeding antibiotics that added in the daily ration of broiler prescription fully.
For showing that effect of the present invention carries out following contrast test:
Test one: lactulose is to the influence of three yellow chicken production performances
1, experimental animal and grouping
Select 144 of the three yellow meat chicks of 1 close age in days health of body weight for use, be divided into 4 groups at random, every group of 3 repetitions, each repeats 12 chickens.
2, date of test
Growth test was from May 31,5 days to 2010 April in 2010,56 days by a definite date.
3, feeding and management
The feeding manner that test adopts the cage male and female to raise together with, brood time adopts the stove heat tracing of brooding.Illumination (24h), temperature and immunity are carried out according to routine.Whole test phase free choice feeding is freely drunk water.First week was given up 33 ℃ to 35 ℃ of temperature, descended 2 ℃ weekly, 4 week back natural temperatures raisings (18 ℃ to 22 ℃ of preference temperatures) down.
4, the addition and the adding method that contain feeding antibiotic substitute of the present invention
Test divides 4 groups: the control group test fryer basal diet of feeding, that 300 milligrams of western peptide of added with antibiotic, 250 milligrams of colistine sulfates in every kilogram of basal diet.Test 1 group, test 2 groups, test 3 groups fryer, interpolation lactulose 5 grams, 10 grams and 15 restrain in every kilogram of basal diet, mix.
5, testing index and assay method
(1) testing the 14th day, 28 days, 42 days, 56 day morning by the body weight that repeats to claim every chicken, is that unit writes down feed intake every day to repeat, if chicken is dead midway, weightening finish and food consumption is proofreaied and correct.Test chicken average daily gain in the statistical test phase, average daily ingestion amount, material anharmonic ratio and the death rate.
6, lactulose is to the influence of three yellow chicken production performances
The interpolation lactulose sees Table 1 to the influence of three yellow chicken production performances.
Add the influence of lactulose in table 1 daily ration to three yellow chicken production performances
By table 1 as seen, respectively organize when 7 age in days to 14 ages in days, 43 age in days to 56 ages in days average daily gain difference not significantly when (P＞0.05), 43 age in days to 56 ages in days the average weight of 1 group of lactulose respectively than 3 groups high by 4.94% of control group, 2 groups of lactuloses and lactuloses, 20.52% and 11.31%; When 15 age in days to 28 ages in days, 29 age in days to 42 ages in days, the average daily gain significant difference (P＜0.05) of 3 groups of lactuloses and 2 groups of lactuloses, during 8 age in days to 56 ages in days, 2 groups of average daily gains of lactulose respectively with control group, 1 group of lactulose and 3 groups of significant differences of lactulose (P＜0.05), and respectively than control group, 1 group of lactulose and 3 groups of bases of a fruit of lactulose 12.12,12.69 and 12.48%.
From the material anharmonic ratio, during 29 age in days to 42 ages in days 2 groups of lactuloses respectively with control group, 1 group of lactulose and 3 groups of material anharmonic ratioes of lactulose difference extremely significantly (P＜0.01) and respectively than 3 groups high by 9.72% of control group, 1 group of lactulose and lactuloses, 12.32% and 12.86%; 2 groups of lactuloses and 3 groups of material anharmonic ratioes of lactulose significant difference (P＜0.05), control group material anharmonic ratio are respectively than 3 groups high by 3.06% of 1 group of lactulose, 2 groups of lactuloses and lactuloses, 0.85% and 8.76% during 8 age in days to 56 ages in days.Material anharmonic ratio difference all not significantly (P＞0.05) when 7-14 age in days, 15-28 age in days, 43-56 age in days.
Test two: the influence of lactulose dialogue plumage meat chick production performance
1, experimental animal and grouping
Select 180 of the AA meat chicks of 1 close age in days health of body weight for use, be divided into 4 groups at random, every group of 3 repetitions, each repeats 15 chickens.
2, date of test
Growth test was from July 31,20 days to 2010 June in 2010,42 days by a definite date.
3, feeding and management
The feeding manner that test adopts the cage male and female to raise together with, brood time adopts the stove heat tracing of brooding.1 age in days hypodermic injection horse Garrick vaccine is carried out in illumination (24h), temperature and immunity according to routine; 7 ages in days carry out bronchitis ewcastle disease bigeminy seedling collunarium head exempts from, the weak malicious seedling drinking-water of the 14 age in days bursa of farbricius, the Newcastle disease attenuated seedling booster immunization of 21 ages in days.Whole test phase free choice feeding is freely drunk water.First week was given up 33 ℃ to 35 ℃ of temperature, descended 2 ℃ weekly, 4 week back natural temperatures raisings (18 ℃ to 22 ℃ of preference temperatures) down.
4, the addition and the adding method that contain feeding antibiotic substitute of the present invention
Test divides 4 groups: control group is: the antibiotic group of basal diet+antibiotic (the western peptide of that of 0.03% and 0.25% colistine sulfate pre-mixing agent); Test I group is: the glutamine+later stage (22d) of (21d) interpolation 0.1% is added 1% lactulose group in earlier stage; Test II group is: the lactulose group of 0.1% glutamine+1%; Test III group is: basal diet+1% lactulose mixes.
5, testing index and assay method
Testing the 7th day, 21 days, 35 days, 42 day morning by the body weight that repeats to claim every chicken, is that unit writes down feed intake every day to repeat, if chicken is dead midway, weightening finish and food consumption is proofreaied and correct.Test chicken average daily gain in the statistical test phase, average daily ingestion amount, material anharmonic ratio and the death rate.
6, lactulose is to the influence of meat chicken production performance
The interpolation lactulose sees Table 2 to the influence of meat chick production performance.
By table 2 as seen, the meat chick is during the 1-21 age in days, and 21d glutamine+22d lactulose group is higher by 3.99% than the average daily gain of antibiotic group, glutamine+lactulose group, lactulose group respectively, 0.80% and 4.75%, but all do not have significant difference between each group; During the 22-35 age in days, the average daily gain of each group does not all have significant difference; During the 36-42 age in days, glutamine+lactulose group is higher by 27.10% than the average daily gain of antibiotic group, significant difference (P＜0.05), and difference is not remarkable between all the other each groups; Full phase average daily gain result shows that glutamine+lactulose group average daily gain effect is best, but the no significant difference between each group.
From the material anharmonic ratio, the meat chick is during the 1-21 age in days, the average material anharmonic ratio of lactulose group is lower than antibiotic group 13.99%, difference is (P＜0.01) extremely significantly, the average material anharmonic ratio of 21d glutamine+22d lactulose group is lower than antibiotic group 8.00%, and the average material anharmonic ratio of glutamine+lactulose group is lower than antibiotic group 6.14%, but difference is not remarkable; 22 age in days to 35 ages in days, 36 age in days to 42 ages in days and full phase, control group and each test group difference are not remarkable, and difference is not remarkable yet between each test group.
This test shows, does not make significant difference with the production performance of the alternative feeding antibiotic of lactulose to fryer in daily ration of broiler.
Add the influence of lactulose in table 2 daily ration to meat chick production performance
Major advantage of the present invention
(1) substitute feeding antibiotic in the daily ration of broiler fully, have feeding antibiotic equally and had can guarantee fryer health, promote growth of meat chicken, the effect that improves feed efficiency.
(2) eliminate the drug resistance that the fryer enteric bacteria produces feeding antibiotic.
(3) eliminate the medicament residue of feeding antibiotic in fryer product and environment.
(4) regulate fryer intestinal microecology balance, reduce owing to the immunological stress state that infringement caused of harmful microorganism to fryer.
(5) promote broiler chicks small intestine, big intestinal epithelial cell growth, improve the defence capability of enteron aisle.
Main feature of the present invention
(1) can substitute feeding antibiotic in the daily ration of broiler fully.
(2) adding proportion is low.
Technical characterstic
Lactulose can stimulate the absorption of the propagation and the nutritional labeling of beneficial bacterium, suppresses pathogen breeding and field planting, strengthens the anti-infectious immunity ability; Reduce the effect of the factors such as phytolectin of environment in being unfavorable for, evenly add in the daily ration of broiler and can regulate fryer intestinal microecology balance behind the alternative feeding antibiotic, the SCFA that the fermentation back produces in large intestine can promote intestinal epithelial cell production, improve the defence capability of enteron aisle, reduce owing to the immunological stress state that infringement caused of harmful microorganism to fryer.Glutamine is the energy substance of small intestine, adds glutamine and can promote broiler chicks mucous membrane of small intestine epithelial cell growth in daily ration, improves the defence capability of enteron aisle.Use method of the present invention, can obtain assurance fryer health that feeding antibiotic has equally, promote growth of meat chicken, the effect that improves feed efficiency, can be used for not adding the production of the fryer and products thereof of any feeding antibiotic.
The specific embodiment
Glutamine+lactulose substitutes the usage of feeding antibiotic in the daily ration of broiler, its addition and adding method are as follows: 1) adding proportion is in the basal diet of three yellow chicken different growing stages: add lactulose 5 grams in per 1 kilogram of basal diet to 15 grams, mix; (2) adding proportion is in the basal diet of white plumage fryer different growing stages: fryer 0 to 21 day, add glutamine 1 gram in per 1 kilogram of basal diet; Fryer 22 days to 42 days adds lactulose 5 grams to 15 grams in per 1 kilogram of basal diet, mix.Fryer 0 to 42 day adds glutamine 1 gram, lactulose 5 grams to 15 grams in per 1 kilogram of basal diet, mix.Fryer 0 to 42 day adds lactulose 5 grams to 15 grams in per 1 kilogram of basal diet, mix.
Claims (5)
1. the plants essential oil of an alternative daily ration of broiler feeding antibiotic is characterized in that: lactulose and glutamine.
2. the lactulose of alternative daily ration of broiler feeding antibiotic according to claim 1 is characterized in that: three yellow chickens, add lactulose 5 grams in per 1 kilogram of basal diet to 15 grams, mix.
3. the lactulose of alternative daily ration of broiler feeding antibiotic according to claim 1 is characterized in that: the white plumage fryer at 0 to 42 day, add lactulose 10 grams in per 1 kilogram of basal diet, and mix.
4. the lactulose of alternative daily ration of broiler feeding antibiotic according to claim 1 and glutamine, it is characterized in that: adding proportion is in the basal diet of white plumage fryer different growing stages: fryer 0 to 21 day, add glutamine 1 gram in per 1 kilogram of basal diet, mix; Fryer 22 days to 42 days adds lactulose 10 grams in per 1 kilogram of basal diet, mix.
5. the lactulose of alternative daily ration of broiler feeding antibiotic according to claim 1 and glutamine is characterized in that: fryer 0 to 42 day, add glutamine 1 gram, lactulose 10 grams in per 1 kilogram of basal diet, and mix.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110582207A (en) * | 2018-12-18 | 2019-12-17 | 黄华成 | Application of glutamine derivative in preparation of animal feed additive |
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| CN1325633A (en) * | 2000-05-26 | 2001-12-12 | 味之素株式会社 | Forages for livestocks |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1325633A (en) * | 2000-05-26 | 2001-12-12 | 味之素株式会社 | Forages for livestocks |
Non-Patent Citations (2)
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| 冉小波等: "乳果糖的作用及在动物饲养中的应用", 《饲料工业》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110582207A (en) * | 2018-12-18 | 2019-12-17 | 黄华成 | Application of glutamine derivative in preparation of animal feed additive |
| CN110582207B (en) * | 2018-12-18 | 2022-11-11 | 广州英赛特生物技术有限公司 | Application of glutamine derivative in preparation of animal feed additive |
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Application publication date: 20110216 |