CN101967480A - Molecular marker relevant to chicken skin color and authentication method and application thereof - Google Patents
Molecular marker relevant to chicken skin color and authentication method and application thereof Download PDFInfo
- Publication number
- CN101967480A CN101967480A CN 201010531819 CN201010531819A CN101967480A CN 101967480 A CN101967480 A CN 101967480A CN 201010531819 CN201010531819 CN 201010531819 CN 201010531819 A CN201010531819 A CN 201010531819A CN 101967480 A CN101967480 A CN 101967480A
- Authority
- CN
- China
- Prior art keywords
- chicken
- skin
- skin color
- molecular marker
- base
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention provides a molecular marker relevant to chicken skin color, which is an A/G basic group mutation located in the 259th basic group in the sequence disclosed in SEQ ID NO:1. The invention also provides an authentication method of the molecular marker and an application thereof in molecular marker assisted selection on the aspect of chicken skin color characters. The molecular marker takes the DNA sequence of a chicken BCDO2 gene and selects primers shown in SEQ ID NO:2 and SEQ ID NO:3 for amplification. The detection method used by the invention has high accuracy and low cost. The detection of the A/G mutantsite can know the gene type of the character of the target chicken flock skin color so as to select chicken flocks on purpose and improve the consistency between chicken flock skin color and shank color.
Description
Technical field
The present invention relates to chicken marker assisted selection technical field, specifically, relate to a kind of relevant molecule marker and discrimination method and application of cock skin skin color that is used for the chicken marker assisted selection.
Background technology
Skin color is to be used to one of important indicator of estimating bird product economy value, the yellow of cock skin skin mainly is that this deposition capability is subjected to the influence of aspects such as heredity, feed nutrition, fodder additives, feeding environment, healthy state owing to sedimentary results of yellow pigment such as carotenoid and xenthophylls.The performance of xenthophylls mainly is by W in the cock skin skin epidermal area
+, this locus of w determined that this gene is positioned on the number one karyomit(e), belongs to the 3rd linkage group.The dominant gene of wild-type can hinder the deposition of yellow pigment on positions such as skin, pin shin, beak and fat, makes skin, pin shin color present white; The recessive gene of relative mutant then allows yellow pigment in positions such as skin, pin shin, beak and fat deposition, makes these positions present yellow.Research is at present found, the major function of BCDO2 (the beta-carotene dioxygenase 2) gene of coding β-Hu Luobusu dioxygenase 2 is that coloured carotenoid is decomposed into colourless material, thereby make the cock skin skin not have the yellow pigment deposition, and become pale skin.Research proves that also the sudden change on this gene is relevant with cock skin skin color, but at present from the angle of heredity, also not setting up a kind of effective differentiation chicken is yellow skin or pale skin, and pale skin is heterozygote or homozygous method.
Summary of the invention
One of purpose of the present invention provides the relevant molecule marker of a kind of cock skin skin color.This molecule marker is positioned on the exon of cock skin skin color related gene B CDO2.Specifically, this molecule marker is arranged in the 259th A/G of bit base place base mutation of sequence shown in the SEQ ID NO:1.When this place's base was A, then cock skin skin color was a white, and when this place's base was G, then cock skin skin color was yellow.
The present invention based on the DNA full length sequence of cock skin skin color related gene B CDO2, seek the mutational site and the corresponding pleiomorphism detecting method that influence skin color on this gene, by the checking in a plurality of colonies, for chicken breeding provides a kind of molecular identification method about its skin color.Below be concrete technical scheme:
Dna sequence dna (reference sequences GeneBank accession number is NC 006111.2) with chicken BCDO2 gene is a template, this Gene Partial sequence of design primer amplification, and used primer is:
Forward primer: 5 '-TCCTCTGATTGCTTTACTGACTTG-3 ',
Reverse primer: 5 '-GGGAAGGAGGTATCATTGGAGA-3 '.
The sequence that obtains of increasing comprise the 6th complete exon and part the 5th intron, part the 6th intron, as described in sequence table SEQ ID NO:1, this sequence total length is 444bp.
The multisequencing compare of analysis of the present invention by the above-mentioned dna fragmentation that obtains being carried out (comprise pale skin chicken and yellow skin chicken) between chicken breed, the sequence of acquisition as shown in Figure 2.Comparison result is found a kind of molecule marker that the chicken marker assisted selection is used that can be used as, and this mark is relevant with the skin color of chicken, has the A/G sudden change at the 259th bit base place of this sequence, causes Sdu I-RFLP polymorphism.
Second purpose of the present invention provided the discrimination method of this molecule marker, is template with the dna sequence dna of chicken BCDO2 gene, selects following primer to increase:
Forward primer: 5 '-TCCTCTGATTGCTTTACTGACTTG-3 ',
Reverse primer: 5 '-GGGAAGGAGGTATCATTGGAGA-3 ';
The resulting product utilization Sdu of pcr amplification I restriction endonuclease is carried out endonuclease reaction, agarose gel electrophoresis detects enzyme and cuts product, if having only the band of a 444bp, then the dna double chain is the A base at this place, mutational site, be judged as the AA genotype, show as pale skin; If 2 bands that are respectively 263bp and 181bp are arranged, illustrate that then the dna double chain is the G base at this place, mutational site, be judged as the GG genotype, show as yellow skin; If 3 bands that are respectively 444bp, 263bp and 181bp are arranged, illustrate that then the dna double chain is the A base at this chain in place, mutational site, another chain is the G base, is judged as the AG genotype, because A allelotrope is dominance, shows as pale skin.
The 3rd purpose of the present invention provides the application of this molecule marker in the chicken molecular marker assisted selection, and particularly cock skin skin color is selected the application in the breeding.
By technical scheme provided by the present invention, can also be prepared into the identification reagent box of this molecule marker.
The invention has the beneficial effects as follows,, can know the genotype of this proterties of target chicken group skin color, thereby can have purpose that the chicken group is selected, improve the consistence of its offspring chicken group's skin and shin look by detection to above-mentioned A/G mutational site.In addition, detection method accuracy height used in the present invention, cost is low, efficient is high.
Description of drawings
Fig. 1 is the partial dna sequence on the chicken BCDO2 gene among the embodiment 1, is used for seeking the segmental electrophoresis picture of molecule marker;
Fig. 2 uses the result that method of the present invention is compared to this fragment on the BCDO2 gene of 2 pale skin chickens and 2 yellow skin chickens among the embodiment 1;
Fig. 3 is three kinds of genotype (AA, AG and the GG) electrophoretograms (agarose gel concentration is 1.5%) of the Sdu I-RFLP on the chicken BCDO2 gene extron 6 among the embodiment 1.
Embodiment
For making the present invention easier to understand,, further set forth the present invention below in conjunction with specific embodiment.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.
Embodiment 1: the foundation of cock skin skin color molecular detecting method
(1) primer:
Dna sequence dna (reference sequences GeneBank accession number is NC 006111.2) with chicken BCDO2 gene is a template, design a pair of primer YSD-F/YSD-R and clone the dna sequence dna (comprising the 6th complete exon and part the 5th intron, part the 6th intron) that obtains shown in sequence SEQ ID NO:1, this sequence total length is 444bp.Used primer sequence is respectively shown in SEQ ID NO:2 and SEQ ID NO:3, and is specific as follows:
YSD-F：5′-TCCTCTGATTGCTTTACTGACTTG-3′，
YSD-R：5′-GGGAAGGAGGTATCATTGGAGA-3′。
(2) pcr amplification condition:
Cumulative volume is to add dna profiling 1 μ L in the PCR reaction system of 25 μ L, each 1 μ L before and after 2 * PCR reaction mixture, the 12.5 μ L, 10mM primer, and Taq archaeal dna polymerase 0.625U, distilled water 8.25 μ L, the PCR reaction conditions is: 94 ℃ of pre-sex change 3min; 94 ℃ of sex change 30s, 56 ℃ of annealing 30s, 72 ℃ are extended 30s, totally 33 circulations; Last 72 ℃ are extended 10min, 4 ℃ of preservations.
(3) seek molecule marker:
Choose the blood DNA of 2 pale skin chickens and 2 yellow skin chickens and do template, carry out pcr amplification, the PCR product detects through 1.5% agarose gel electrophoresis, as shown in Figure 1.Fig. 1 is the partial dna sequence on the chicken BCDO2 gene, be used for seeking the segmental electrophoresis picture of molecule marker, clip size is 444bp (agarose gel concentration is 1.5%), wherein, the M swimming lane is a dna molecular amount standard (DL2000), B1, B2 represent pale skin chicken BCDO2 gene PCR amplified fragments, and H1, H2 represent yellow skin chicken BCDO2 gene PCR amplified fragments.The PCR product is reclaimed, clones, checks order the nucleotide sequence of its sequencing sequence shown in H1, H2, B1, B2 among Fig. 2.Find to exist 8 SNP of place by Cluster W comparison, the deletion mutantion of 1 place, at 259 places base mutation has taken place with pale skin chicken comparison discovery yellow skin chicken, sported G by A, the position of this mutational site on BCDO2 genome sequence (the GeneBank accession number is NC 006111.2) is 14253 places.Specifically see Fig. 2, Fig. 2 is the result that this fragment on the BCDO2 gene of 2 pale skin chickens and 2 yellow skin chickens is compared.B1, B2 represent the pale skin chicken among the figure, and H1, H2 represent the yellow skin chicken, and among the figure shown in the square frame is the A/G mutational site that this sequence exists at 259 bit base places, and the sequence shown in the underscore is the primer sequence that the present invention designs.Experiment concrete operations step is as follows:
1, the purifying of PCR product: under ultraviolet lamp, contain the segmental gel of purpose from the cutting-out of low melting-point agarose gel, put into 1.5mL Ependorff pipe, use PCR product purification test kit (available from Promega company) purified pcr product then, according to this test kit specification sheets operation.
2, ligation: the PCR product and the pMD18-T Vector (available from TaKaRa company) of purifying are connected, and the ligation cumulative volume is 10 μ L, connects damping fluid, 2.5 μ L comprising 2 * fast; Carrier (50ng), 0.5 μ L; Reclaim DNA, 7-8 μ L.Bullet is beaten and is mixed a little, connects more than the 1h or 4 ℃ of connections are spent the night in 16 ℃.
3, the preparation of competent cell: from 37 ℃ of incubators, cultivated on the fresh flat board of 16-20h the single colony inoculation of DH5 α of picking in 2mL LB, in 37 ℃ of shaking table shaking culture 3h, switching 1mL bacterium liquid is in the saline bottle that contains 300mL LB, continuation is at 37 ℃ of about 4h of shaking table shaking culture, treat that when OD600 reaches 0.3-0.4 saline bottle being put ice bath from the shaking table taking-up cools off 10-15min, then bacterium liquid is changed in the centrifuge tube in 4 ℃ 4, the centrifugal 10min of 000g is with collecting cell, centrifuge tube is inverted to abandon clean nutrient solution, is iced the CaCl of the 0.1mol/L of precooling with 10mL
2Resuspended precipitation, ice bath 30min repeats 4 ℃ 4, and the centrifugal 10min of 000g once ices the CaCl of the 0.1mol/L of precooling with 4ml
2Resuspended precipitation, it is standby to put 4 ℃ of preservations.
4, transform: in the 1.5mL centrifuge tube of sterilization, add 100 μ L competent cells, add on ice and connect product 5 μ L, with liquid-transfering gun piping and druming evenly, ice bath 30min; Centrifuge tube is placed 42 ℃ circulator bath (not vibrations), behind the heat shock 90s, rapid ice bath 2min; In centrifuge tube, add 400 μ L LB nutrient solutions again, bathe recovery 45-60min in 37 ℃ of shaking tables (200rpm/min) temperature; Centrifugal, remove the part supernatant liquor, the bacterium liquid of getting after 100 μ L recover is distributed on the flat board that contains Amp, smoothens; After treating that liquid fully absorbs, be inverted plate, in 37 ℃ of cultivations 14-16 hour, observation had or not bacterium colony to grow.
5, the evaluation of positive colony and order-checking: the bacterial plaque that picking transforms from flat board inserts the 1.5mL centrifuge tube that contains 400 μ L LB and in 37 ℃ of shaking table joltings and cultivates about about 8h.With this bacterium liquid is template, selects for use the universal primer M13 (Beijing Liuhe Huada Genomics Technology Co., Ltd provides) of former primer or order-checking to carry out pcr amplification (annealing temperature 58-60 ℃).Electrophoresis detection, the bacterium liquid of picking positive colony is sent to order-checking, and sequencing is finished by last Beijing Liuhe Huada Genomics Technology Co., Ltd.
In this side's of enforcement example, pcr amplification product is special PCR product (Fig. 1) through the demonstration of 1.5% agarose gel electrophoresis detected result, Fig. 1 is the partial dna sequence on the chicken BCDO2 gene among the embodiment 1, be used for seeking the segmental electrophoresis picture of molecule marker, clip size is 444bp (agarose gel concentration is 1.5%), wherein, the M swimming lane is a dna molecular amount standard (DL2000).With the recovery of PCR product, purifying rear clone, order-checking (comparison chart of measured sequence is seen Fig. 2), Fig. 2 uses the result that method of the present invention is compared to this fragment on the BCDO2 gene of 2 pale skin chickens and 2 yellow skin chickens, wherein B1, B2 represent pale skin chicken aligned sequences, H1, H2 represent yellow skin chicken aligned sequences, and square frame is depicted as the A/G mutational site that this sequence exists at 259 bit base places.The result shows that resulting dna sequence dna length is 444bp, comprises the 6th complete exon, and part the 5th intron and part the 6th intron sequences (shown in sequence table SEQ ID NO:1); Sequencing result shows at 259 places of this dna sequence dna and has the A/G base mutation.
(4) the PCR-RFLP detection method is set up:
The A/G base mutation at these fragment 259 places, just can be discerned by restriction endonuclease Sdu I, with above-mentioned PCR product 6 μ L, 10 * damping fluid, 1 μ L, restriction enzyme Sdu I 0.2 μ L (2U), adding distilled water mends to 10 μ L, with centrifugal behind the sample mixing, 37 ℃ of incubators are placed 6h, detect with 1.5% agarose gel electrophoresis, imaging system is observed enzyme and is cut the result, the record genotype.This gene mutation site is controlled by two allelotrope, and wherein A is the allelotrope that does not form restriction enzyme site, and G is the allelotrope that forms restriction enzyme site.These two allelotrope can be formed three kinds of genotype, wherein homozygous (the having only DNA band of 444bp during electrophoresis detection) that enzyme is cut for not taking place in the AA type, homozygous (the occurring two DNA bands of 263bp and 181bp during electrophoresis detection) that enzyme is cut for taking place in the GG type, AG is heterozygous (occurring three DNA bands of 444bp, 263bp and 181bp during electrophoresis detection), sees Fig. 3 for details.Fig. 3 is three kinds of genotype (AA, AG and the GG) electrophoretograms (agarose gel concentration is 1.5%) of the Sdu I-RFLP on the chicken BCDO2 gene extron 6 among the present invention, and the M swimming lane is a dna molecular amount standard (DL2000).
The detection and the application of embodiment 2:PCR-Sdu I-RFLP polymorphism distribution situation in each chicken strain
Having collected different shin looks and the skin-color chicken blood DNA of totally 3 strains in this example, is respectively B, Z, H totally 3 strains, and sample standard deviation is from Guangdong intelligence prestige agricultural science and technology company limited by shares.According to embodiment 1 described method above chicken kind has been carried out PCR-Sdu I-RFLP and detected, to verify the molecular diagnosis method of this kind of the present invention about cock skin skin color.Detected result is as shown in table 1.Table 1 is the distribution detected result of BCDO2 gene Sdu I-RFLP polymorphism in the chicken group.
Table 1
As shown in Table 1, the genotype of the individuality that is detected all conforms to its skin color, illustrates that method of the present invention is reliable, effective.
Molecule marker of the present invention can be applicable to cock skin skin color and selects in the breeding.As the Z Strains of Chickens that table 1 detected, its macroscopic features is that skin color is a white, the shin look is a black, and find that in our testing process some pale skin individualities are AG heterozygous genes type in its colony, if the stud mating of AG heterozygous genes type so, the GG genotype will occur in its offspring colony, show as yellow individuality, and yellow skin is the macroscopic features that does not meet this strain of Z.The individuality of this strain father, female Dai Zhongwei AG heterozygous genes type can be weeded out so use molecule marker of the present invention,, make colony's unanimity aspect this macroscopic features of skin color to guarantee not have among its offspring chicken group the appearance of yellow skin individuality.
Last institute should be noted that; above embodiment is only in order to illustrate technical scheme of the present invention but not limiting the scope of the invention; although the present invention has been done detailed description with reference to preferred embodiment; those of ordinary skill in the art is to be understood that; can make amendment or be equal to replacement technical scheme of the present invention, and not break away from the essence and the scope of technical solution of the present invention.
Sequence table
＜120〉relevant molecule marker and discrimination method and the application of a kind of cock skin skin color
<160>3
<170>PatentIn?version?3.2
<210>1
<211>444
<212>DNA
<213>chicken
<222>(1)...(444)
<400>1
tcctctgatt?gctttactga?cttgataact?gtttctttat?tctttttttt?tctgcccagt 60
tatgtaacta?ctgtatttct?acctacacta?aggaaataga?aaagcactga?gcctgacttt 120
atctttgtga?atctgggcag?cttcacatct?cagtggtgct?gaaccccctt?ttttctttcc 180
agggactaca?tataatataa?ttgaggtccc?tccacaaaag?tcgaactgca?atgagacctt 240
agagggagca?aaagtgctat?gctccattgc?tccaacagat?aacatgaaac?cctcctacta 300
tcacagcttt?ggtaagaatg?cttcctgtct?catactccat?ggatttagcc?tcctgtgcag 360
cacaaggggc?tgttaactgc?cctctgtttc?aatttttttt?gttgttgttt?catttgccaa 420
gttctccaat?gatacctcct?tccc 444
<210>1
<211>24
<212>DNA
＜213〉artificial sequence
<222>(1)...(24)
<400>2
tcctctgatt?gctttactga?cttg 24
<210>1
<211>22
<212>DNA
＜213〉artificial sequence
<222>(1)...(22)
<400>3
gggaaggagg?tatcattgga?ga 22
Sequence table
Claims (4)
1. the molecule marker that cock skin skin color is relevant is characterized in that described molecule marker is the 259th A/G of a bit base place base mutation in the sequence shown in SEQID NO:1.
2. differentiate the described molecular marker method of claim 1 for one kind, it is characterized in that may further comprise the steps: the dna sequence dna with chicken BCDO2 gene is a template, selects following primer amplification:
Forward: 5 '-TCCTCTGATTGCTTTACTGACTTG-3 ',
Oppositely: 5 '-GGGAAGGAGGTATCATTGGAGA-3 ';
The gained amplified production is cut with restriction enzyme Sdu I enzyme,
If have only a band, then this place, mutational site is the A base;
If two bands are arranged, then the place, mutational site is the G base;
If three bands are arranged, illustrate that then this chain in place, mutational site is the A base, another chain is the G base.
3. the application in the molecular marker assisted selection of the described molecule marker of claim 1 aspect cock skin skin color proterties.
4. application according to claim 3 is characterized in that, comprises the application in the cock skin skin color selection breeding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010531819 CN101967480B (en) | 2010-11-04 | 2010-11-04 | Molecular marker relevant to chicken skin color and authentication method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010531819 CN101967480B (en) | 2010-11-04 | 2010-11-04 | Molecular marker relevant to chicken skin color and authentication method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101967480A true CN101967480A (en) | 2011-02-09 |
| CN101967480B CN101967480B (en) | 2013-05-15 |
Family
ID=43546703
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010531819 Active CN101967480B (en) | 2010-11-04 | 2010-11-04 | Molecular marker relevant to chicken skin color and authentication method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101967480B (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103834642A (en) * | 2014-02-14 | 2014-06-04 | 华南农业大学 | Chicken skin color related molecular marker and its application |
| CN105603094A (en) * | 2016-02-22 | 2016-05-25 | 杭州市农业科学研究院 | SNP molecular marker related to skin color character of black-bone chicken and application |
| CN110157814A (en) * | 2019-05-31 | 2019-08-23 | 江苏省家禽科学研究所 | One kind SNP marker relevant to chicken leg portion skin follicle density character and its detection method and application |
| CN111876492A (en) * | 2020-07-15 | 2020-11-03 | 华南农业大学 | Molecular marker influencing skin color of shank of chicken and application thereof |
| CN112899374A (en) * | 2021-03-04 | 2021-06-04 | 华南农业大学 | Molecular marker related to skin color of chicken, primer group and application |
| CN113218884A (en) * | 2021-04-29 | 2021-08-06 | 华南农业大学 | Breeding method for chicken skin yellowness |
| CN115181805A (en) * | 2022-02-23 | 2022-10-14 | 华南农业大学 | Molecular marker related to yellow degree of leg skin of yellow-feathered broilers and application of molecular marker |
| CN115521984A (en) * | 2021-06-25 | 2022-12-27 | 华中农业大学 | Method for identifying nondestructive sexes in middle stage of chicken embryo by utilizing tibia heredity and transmission spectrum |
| CN115992264A (en) * | 2023-02-21 | 2023-04-21 | 华中农业大学 | Chicken yellow and white skin color molecular identification primer group and method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1730666A (en) * | 2004-08-06 | 2006-02-08 | 张沅 | Method for detecting chicken fat property using mononucleotide polymorphism |
| WO2009046724A2 (en) * | 2007-10-08 | 2009-04-16 | Aarhus Universitet | Polymorphisms of mbl |
| US20090246778A1 (en) * | 2003-12-16 | 2009-10-01 | University Of Delaware | Identification of fat and lean phenotypes in chickens using molecular markers |
| CN101696452A (en) * | 2009-10-19 | 2010-04-21 | 华中农业大学 | Method for identifying molecules of recessive white feather genes of chicken |
-
2010
- 2010-11-04 CN CN 201010531819 patent/CN101967480B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090246778A1 (en) * | 2003-12-16 | 2009-10-01 | University Of Delaware | Identification of fat and lean phenotypes in chickens using molecular markers |
| CN1730666A (en) * | 2004-08-06 | 2006-02-08 | 张沅 | Method for detecting chicken fat property using mononucleotide polymorphism |
| WO2009046724A2 (en) * | 2007-10-08 | 2009-04-16 | Aarhus Universitet | Polymorphisms of mbl |
| CN101696452A (en) * | 2009-10-19 | 2010-04-21 | 华中农业大学 | Method for identifying molecules of recessive white feather genes of chicken |
Non-Patent Citations (2)
| Title |
|---|
| 《The Journal of Heredity》 20021231 Nagaraja S等人 Trait association of a genetic marker near t he IGF2 I gene in egg laying chickens 150-156 1-4 第91卷, 第2期 2 * |
| 《中国畜牧兽医》 20081231 梁敏等人 鸡QTL定位的研究进展 85-87 1-4 第35卷, 第11期 2 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103834642B (en) * | 2014-02-14 | 2016-06-29 | 华南农业大学 | Molecular marker that a kind of chicken skin color is relevant and application thereof |
| CN103834642A (en) * | 2014-02-14 | 2014-06-04 | 华南农业大学 | Chicken skin color related molecular marker and its application |
| CN105603094A (en) * | 2016-02-22 | 2016-05-25 | 杭州市农业科学研究院 | SNP molecular marker related to skin color character of black-bone chicken and application |
| CN105603094B (en) * | 2016-02-22 | 2019-01-04 | 杭州市农业科学研究院 | A kind of relevant SNP marker of black-bone chicken colour of skin character and application |
| CN110157814B (en) * | 2019-05-31 | 2022-05-10 | 江苏省家禽科学研究所 | SNP molecular marker related to drumstick skin hair follicle density character and detection method and application thereof |
| CN110157814A (en) * | 2019-05-31 | 2019-08-23 | 江苏省家禽科学研究所 | One kind SNP marker relevant to chicken leg portion skin follicle density character and its detection method and application |
| CN111876492A (en) * | 2020-07-15 | 2020-11-03 | 华南农业大学 | Molecular marker influencing skin color of shank of chicken and application thereof |
| CN112899374A (en) * | 2021-03-04 | 2021-06-04 | 华南农业大学 | Molecular marker related to skin color of chicken, primer group and application |
| CN112899374B (en) * | 2021-03-04 | 2022-06-07 | 华南农业大学 | Molecular marker related to skin color of chicken, primer group and application |
| CN113218884A (en) * | 2021-04-29 | 2021-08-06 | 华南农业大学 | Breeding method for chicken skin yellowness |
| CN115521984A (en) * | 2021-06-25 | 2022-12-27 | 华中农业大学 | Method for identifying nondestructive sexes in middle stage of chicken embryo by utilizing tibia heredity and transmission spectrum |
| CN115181805A (en) * | 2022-02-23 | 2022-10-14 | 华南农业大学 | Molecular marker related to yellow degree of leg skin of yellow-feathered broilers and application of molecular marker |
| CN115181805B (en) * | 2022-02-23 | 2023-08-11 | 华南农业大学 | Molecular marker related to yellow-feather broiler leg skin yellowness and application thereof |
| CN115992264A (en) * | 2023-02-21 | 2023-04-21 | 华中农业大学 | Chicken yellow and white skin color molecular identification primer group and method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101967480B (en) | 2013-05-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101967480B (en) | Molecular marker relevant to chicken skin color and authentication method and application thereof | |
| CN106086229B (en) | The relevant molecular labeling of chicken growth traits and its discrimination method and application | |
| CN104805115A (en) | Plant genomic DNA flanking SPT event and methods for identifying SPT event | |
| CN111394445B (en) | Indel marker for sex identification of channa maculata and application thereof | |
| CN104630341B (en) | The genes of Chinese Simmental FGF 1 make the genetic marker of Carcass meat quality | |
| CN105567865B (en) | SNP marker relevant to Marsupenaeus japonicus heat resistance and its detection method | |
| CN101440399B (en) | Molecular marking method for indicating and identifying litter size in pigs by MMP23 gene | |
| CN112760385B (en) | SNP (Single nucleotide polymorphism) marker related to beef character and application thereof | |
| CN104059963A (en) | Detection method of Chinese simmental cattle carcass and meat quality trait genetic markers | |
| CN102634522A (en) | Gene for controlling rice fertility, encoded protein and application thereof | |
| CN113584079A (en) | Establishment of zebra fish heart specific marker strain applied to calcium ion imaging | |
| CN112029795B (en) | Application of MpICE1 transcription factor in improving plant disease resistance | |
| CN102649811B (en) | Protein TaWRKY2 related to powdery mildew resistance and coding gene of protein TaWRKY2 | |
| CN106119407A (en) | LAP3 gene is as the molecular marker of ovine growth character and application thereof | |
| CN103725790A (en) | Molecular marker relevant to growth of fenneropenaeus chinensis and application of molecular marker | |
| CN105695477A (en) | Male sterile mutant oss125 and use thereof | |
| CN109652457A (en) | A kind of method of gene knockout breeding ALPK2 Gene Deletion zebra fish | |
| CN104975097A (en) | Kit for green-eggshell chicken feather pecking related gene detection and implementation method thereof | |
| CN104611349A (en) | FDFT1 gene key loci affecting Chinese simmental cattle fat deposition | |
| CN107574171A (en) | A kind of corn salt resistance main effect QTL and its related gene, molecular labeling and application | |
| CN101955931B (en) | Molecular marker of gene Nudt6 related to pig leaf fat rate, lactone rate and leg breech meat-bone rate and application thereof | |
| CN109652557B (en) | Molecular marker related to swine inguinal scrotal hernia and application thereof | |
| CN104694650B (en) | A kind of molecular detecting method and its application and kit for differentiating chicken feed utilization ratio | |
| CN103725688B (en) | Molecular marker that newcastle disease virus antibody horizontal is relevant and discrimination method thereof and application | |
| CN101245348A (en) | Molecule making clone correlative to production deseription as pig making auxiliary selection and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: ANIMAL HUSBANDRY INST., GUANGDONG PROV. ACADEMY OF Effective date: 20120727 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20120727 Address after: 510640 Guangdong city of Guangzhou province Tianhe District Wushan Guangdong Province Academy of Agricultural Sciences Institute of animal husbandry by building 1 Applicant after: Guangdong Zhiwei Agriculture Technology Co., Ltd. Co-applicant after: Institute of Animal Husbandry, Guangdong Academy of Agricultural Sciences Address before: 510640 Guangdong city of Guangzhou province Tianhe District Wushan Guangdong Province Academy of Agricultural Sciences Institute of animal husbandry by building 1 Applicant before: Guangdong Zhiwei Agriculture Technology Co., Ltd. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |