CN101946696A - Sexual polyploidization breeding method of tetraploid Brassica campestris ssp.chinensis Makino - Google Patents
Sexual polyploidization breeding method of tetraploid Brassica campestris ssp.chinensis Makino Download PDFInfo
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Abstract
The invention belongs to the field of crop breeding and discloses a sexual polyploidization breeding method of tetraploid Brassica campestris ssp.chinensis Makino. The method comprises the following steps of: treating flower buds by using solution of colchicine in the bolting period of the Brassica campestris ssp.chinensis Makino to induce the flower buds to generate 2n male gametes; and performing manual sexual polyploidization cross breeding on a tetraploid male sterile line serving as a female parent and 2n male gamete-containing diploid obtained by the induction of the solution of colchicine serving as a male parent in a blooming period to obtain the tetraploid Brassica campestris ssp.chinensis Makino. In the method, a sexual polyploidization breeding approach of hybridizing the male sterile line of the tetraploid Brassica campestris ssp.chinensis Makino with the diploid Brassica campestris ssp.chinensis Makino which generates 2n gametes after induction is used for the first time to obtain a new species of the tetraploid Brassica campestris ssp.chinensis Makino which has wider genetic diversity and higher seed setting rate compared with the autotetraploid of asexual polyploidization. The new tetraploid of the invention has the characteristics of polyploid such as largeness, disease resistance and cold resistance, high quality, and the properties of a diploid male parent.
Description
Technical field
The invention belongs to the crop breeding field, relate to a kind of sexual polyploidization breeding method of infertile tetraploid Chinese cabbage, be exclusively used in the seed selection of infertile tetraploid Chinese cabbage.
Technical background
Two polyploidization approach are arranged in the natural world spore process.The one, sexual polyploidization, the 2nd, asexual polyploidization, the former is the main path that the natural plant polyploid forms, and promptly by the formation of 2n gamete, participates in sexual mating then.The sexual polyploidization of 2n gamete has more biology advantage, higher occurrence frequency, higher heterozygosity than asexual polyploidization.From infiltrating, create genetic diversity and obtain many times of colonies of genetic heterozygosis from the diplontic gene of nearly edge.Carry out polyploid breeding with the 2n gamete, different genotype is combined on the ploidy level that has improved, for providing possibility to Hyperploidy filial generation transmission heterozygosity and beneficial gene combination, increased the hybrid vigour potentiality, and newtype can be preserved by polyploid heredity from the Hypoploidy parent; In addition, effect is obvious aspect the fecundity raising.The sexual polyploidization of manual operation has become the important means of plant germplasm innovation.
Chinese cabbage (Brassica campestris ssp.chinensis Makino) is commonly called as a variety of Chinese cabbage, Chinese cabbage, green vegetables, and northern China claims " rape ", originates in China.Be China the most popular vegetables in south, in the vegetables year-round supply, occupy critical role.Its natural kind and each cultivated species all are dliploid, i.e. 2n=2x=20.Inventor's induced mutations infertile tetraploid Chinese cabbage " the short pin Huang of Nan Nong " new varieties (Agricultural University Of Nanjing's journal, 1990,13 (2): P.33～40) are first at home and abroad arranged in the prior art; Utilize two tetraploid self incompatible line hybridization to breed tetraploid crossbreed " Youshu No.1 " (patent of invention ZL01137325.3); Initiative infertile tetraploid Chinese cabbage male sterile line and maintenance line (patent of invention ZL03113283.9); Utilize infertile tetraploid Chinese cabbage male sterile line and tetraploid male parent system to produce F
1Can make F for crossbreed (being the optimized hybrid seeding means of hybrid vigour)
1Reach 100% for the hybrid rate.But the infertile tetraploid Chinese cabbage resource-constrained has restricted the heterotic utilization of tetraploid.
The 2n gamete is natural universal phenomenon, and sexual polyploidization is the main path that the natural world polyploid forms.Research is the most deep in potato, potato 2n gamete abiogenesis frequency be 1.3% (bend the application of beautiful .1988.2n gamete of winter in the potato breeding. the potato magazine, 2 (2): 102-105.), in crucifer black mustard 2n gamete abiogenesis frequency be 0.05%～2.17% (Meng Jinling .1997. plant reproductive genetics. Beijing: Science Press, 83-84.), Chinese cabbage 2n gamete abiogenesis frequency is 9.17%～20.06% (Zhang Chenghe, Shen Shuxing, Shang Aiqin, prince is glad, appoint the gametogenic genetic analysis of clear .2007. Chinese cabbage 2n. gardening journal, 27 (4): 295-296.).About utilizing colchicine-induced 2n gamete to obtain the existing report of the research (Zhao Yan of polyploid, Liu Wulin, Zheng is homesick, the sexual polyploidization research of Xiao Yaqiong .2006. colchicine-induced tulip. northern gardening, (6): 135-136. king's generation is quick, Zheng is homesick, gamete mutagenesis of Zhao Yan .2008. Chinese rose and filial generation seed germination research preliminary study. Yunnan Prov Agriculture University's journal, 23 (3): 426-430. Yang Xiao actor, Cheng Zhou, Li Shan, Bei Dao a surname, the Chang Gu river is ploughed two youth .2006. colchicins and is induced pomelo shaddock 2n gamete to obtain the selfing triploid. gardening journal, 33 (5): 1045-1047.).Also do not utilize colchicine-induced dliploid Chinese cabbage to produce the 2n andro gamete in the prior art both at home and abroad at present, with the hybridization of infertile tetraploid Chinese cabbage cytoplasm male sterility line, obtain new tetraploid sexual polyploidization breeding technique again.
Summary of the invention
The objective of the invention is above-mentioned deficiency, a kind of sexual polyploidization breeding method of infertile tetraploid Chinese cabbage is provided at prior art.
Another object of the present invention provides the novel infertile tetraploid Chinese cabbage kind of this method seed selection.
Purpose of the present invention is achieved through the following technical solutions:
A kind of breeding method of infertile tetraploid Chinese cabbage: use colchicine solution to handle bud at dliploid Chinese cabbage peduncle-growing period for rapeseed and induce generation 2n andro gamete; With tetraploid male sterile is maternal, and the dliploid that contains the 2n andro gamete that obtains with described colchicine-induced is that male parent is carried out artificial sexual polyploidization crossbreeding at the florescence, obtains F
1Be described infertile tetraploid Chinese cabbage for tetraploidy Chinese cabbage.
Described breeding method preferably comprises following steps:
1) inducing of 2n andro gamete: the colchicine solution concentration that mutagenesis dliploid Chinese cabbage produces 2n pollen is 0.05% (g/ml, Hereinafter the same), 0.10% and 0.20%, handle bud at dliploid Chinese cabbage peduncle-growing period for rapeseed, twice of every day, bud size 2.5～3.5mm, adopt micro-injection, each bud injection 5～10 μ L, handle once every 6h, each concentration is handled 2 times, is contrast with the plant of being untreated, bagging is isolated then, the 2n pollen characteristic is: oval, average length are 165.27 microns, and mean breadth is 103.72 microns, chromosome number is that (and the n pollen that untreated liploid plant produces is for oval for 2x=20, long 149.70 microns, wide 76.58 microns, chromosome number is x=10);
2) artificial sexual polyploidization hybridization: with tetraploid male sterile is maternal, the dliploid material that contains the 2n gamete with colchicine-induced is a male parent, reaching untreated is that male parent is carried out artificial bud pollination hybridization respectively to impinging upon the florescence, joins 30 strains altogether, and the 3rd day repeats to pollinate 1 time.Early summer results F
1The tetraploid seed.
Wherein, the time that described colchicine solution is handled bud be spring dliploid Chinese cabbage peduncle-growing period for rapeseed 8:00-9:00 in morning and afternoon 2:00-3:00, treatment temperature is respectively 20 ℃-25 ℃ and be not less than 18 ℃.
Described infertile tetraploid Chinese cabbage F through colchicine mutagenesis 2n pollen and sexual polyploidization hybridization acquisition
1Be white vernalization type in April, plant type is uprightly with a tight waist, 28～31 centimetres of plant heights, and 28～34 centimetres of degree of development, the blade subcircular, yellow green, petiole white, flat, individual plant weighs 0.5～0.6 kilogram, compares with dliploid contrast male parent and shows huge property, F
1The plant strain growth gesture is strong, the blade subcircular, leaf-shrinkage, vein is clear, dish is pluckings, the individual plant quality, plant height, degree of development, the number of blade, blade is wide, ten leaf gross masses, blade is long, the petiole quality, petiole is long, petiole is wide, ten leaf gross thickness and petiole thickness rate dliploid increase by 33.81% respectively, 116.95%, 55.68%, 41.54%, 46.15%, 13.43%, 32.18%, 19.81%, 15.91%, 87.24%, 27.55%, 129.79% and 97.72%, the petal color burn, shape becomes circle, longly and wide increase by 82.61% and 103.28% respectively, pore length and widely increase by 93.95% and 71.07% respectively, the angle is really long, horizontal wide, increase by 45.33% respectively with beak is long, 63.33%, with 132.14%, but stomatal frequency reduces 49.58%, and above shape difference reaches utmost point significance level; Through cytological Identification, this sexual its somatic chromosome number of polyploidization tetraploid is 2n=4x=40, and dliploid male parent somatic chromosome number is 2n=2x=20.
The tetraploid male sterile line that described tetraploid male sterile line preferably prepares according to Chinese patent ZL03113283.9.
The preferred dliploid Chinese cabbage of described dliploid Chinese cabbage " brilliant white leaf " (chief editor of Vegetable ﹠ Flower Inst., Chinese Academy of Agriculture Science, China's Vegetable kind will (going up volume), Beijing: 2001 Chinese agriculture publishing houses, 353 pages).
A kind of infertile tetraploid Chinese cabbage kind of utilizing breeding method of the present invention to obtain, infertile tetraploid Chinese cabbage by this kind is a white vernalization type in April, plant type is uprightly with a tight waist, 28～31 centimetres of plant heights, 28～34 centimetres of degree of development, the blade subcircular, yellow green, petiole white, flat, individual plant weighs 0.5～0.6 kilogram, compare with dliploid contrast male parent and to show huge property, infertile tetraploid Chinese cabbage plant strain growth gesture of the present invention is strong, the blade subcircular, leaf-shrinkage, vein is clear, dish is pluckings, the individual plant quality, plant height, degree of development, the number of blade, blade is wide, ten leaf gross masses, blade is long, the petiole quality, petiole is long, petiole is wide, ten leaf gross thickness and petiole thickness rate dliploid increase by 33.81% respectively, 116.95%, 55.68%, 41.54%, 46.15%, 13.43%, 32.18%, 19.81%, 15.91%, 87.24%, 27.55%, 129.79% and 97.72%, the petal color burn, shape becomes circle, longly and wide increase by 82.61% and 103.28% respectively, pore length and widely increase by 93.95% and 71.07% respectively, the angle is really long, horizontal wide, increase by 45.33% respectively with beak is long, 63.33%, with 132.14%, but stomatal frequency reduces 49.58%, and above shape difference reaches utmost point significance level; Through cytological Identification, its Chinese cabbage somatic chromosome number of this tetraploid is 2n=4x=40.
Beneficial effect of the present invention:
It is maternal that the present invention adopts the infertile tetraploid Chinese cabbage male sterile line to do first, both the sexual polyploidization breeding approach of infertile tetraploid Chinese cabbage male sterile line (produces the 2n gamete) * dliploid Chinese cabbage (mutagenesis produces the 2n gamete) itself obtained the infertile tetraploid Chinese cabbage new germ plasm.Compare with the autotetraploid of asexual polyploidization and to have genetic diversity widely, higher ripening rate (ripening rate is low to be the biggest obstacle that artificial polyploid is difficult to use).
In the polyploid research at home and abroad, sexual polyploidization hybridization female parent is all necessary artificial emasculation of dliploid or tetraploid, and time-consuming, its hybrid generation has the selfed seed of original female parent unavoidably, influences result of the test.Adopt male sterile line to do maternal (itself does not have pollen, can not selfing), without artificial emasculation, after the sexual hybridization, the seed of fastening results in male sterile must be a crossbreed.Got rid of the interference of female parent self-cross or pseudostationary.
The present invention adopts variable concentrations colchicine-induced dliploid Chinese cabbage to produce the 2n gamete and makes male parent, makes hybridization of female parent with the infertile tetraploid Chinese cabbage male sterile line again.It is big to obtain new tetraploid probability.
The new tetraploid of the present invention has huge property, disease resistance, the cold resistance of polyploid, quality better, the proterties that also has the dliploid male parent, as late bolting (2～March of the Yangtze river basin, most of a variety of Chinese cabbages all bolting bloom, this tetraploid bolting at the beginning of 4 months is bloomed, and lacks dish to mend late spring), and the good characteristics of commodity (the tetraploid profile is not as dliploid generally speaking) that wait with a tight waist.
Description of drawings
Fig. 1 .n pollen grain, chromosome number x=10 (undressed dliploid Chinese cabbage contrast);
Fig. 2 .2n pollen grain 2x=20 and n pollen grain (two kinds of pollen grains in the dliploid Chinese cabbage of colchicine-induced);
Fig. 3. tetraploid F
1(tetraploid male sterile line and diploid hybrid) tip of a root chromosome number mitosis metaphase (2n=4x=40);
Fig. 4. the pore (* 100) of dliploid contrast;
Fig. 5. tetraploid F
1Pore (* 100);
Fig. 6. relatively (left side is the dliploid contrast to floral organ, and the right side is tetraploid F
1);
Fig. 7. the angle compares really that (left side contrasts for dliploid, and the right side is tetraploid F
1);
Fig. 8. blade relatively (left side is a tetraploid male sterile line female parent, in be tetraploid F
1, right dliploid male parent for inducing);
Fig. 9. plant relatively (left side is a tetraploid male sterile line female parent, in be tetraploid F
1, right dliploid male parent for inducing);
Figure 10. sexual polyploidization hybridization father, female parent and F
1Form anatomy relatively;
Figure 11. sexual polyploidization hybridization father, female parent and F
1Economical character relatively.
Five, embodiment
1 materials and methods
1.1 material
Tested year November in October, 2006-2008 and carry out at Agricultural University Of Nanjing's gardening experiment centre.Dliploid Chinese cabbage " brilliant white the leaf " (chief editor of Vegetable ﹠ Flower Inst., Chinese Academy of Agriculture Science, China's Vegetable kind will (going up volume), Beijing: 2001 Chinese agriculture publishing houses, 353 pages), infertile tetraploid Chinese cabbage male sterile line 08P76 obtains for the artificial asexual polyploidization, preparation method is referring to Chinese patent: ZL03113283.9, with tetraploid " the short pin Huang of the Nan Nong " (chief editor of Vegetable ﹠ Flower Inst., Chinese Academy of Agriculture Science, China's Vegetable kind will (going up volume), Beijing: 2001 Chinese agriculture publishing houses, 353 pages) be contrast, above material provides by Chinese cabbage seminar of gardening institute of Agricultural University Of Nanjing.
1.2 the inducing and identifying of 2n andro gamete
On spring Chinese cabbage peduncle-growing period for rapeseed 8:00-9:00 in the fine morning and on 2:00-3:00 (20 ℃-25 ℃ of daytimes in the afternoon, be not less than 18 ℃ night) processing dliploid Chinese cabbage bud, bud size 2.5～3.5mm (microsporocyte meiosis prophase I), the colchicine concentration of treatment is 0.05%, 0.10% and 0.20%, adopt micro-injection, each bud injection 5～10 μ L, handle once every 6h, each concentration is handled 3 times, each handles 1 strain, major branch and the 1st side shoot (80～90 buds) are handled in every strain, are contrast with the plant of being untreated, and bagging is isolated.The pollen for the treatment of to bloom bud each processing on the same day and contrast getting two flowers mixes respectively, makes 5 smears, and 10 visuals field of each smear random observation, each visual field are measured 3 n and 2n pollen grain respectively and added up pollen variation situation.Total aberration rate=(total pollen number-n pollen number)/total pollen number; 2n gamete frequency=2n pollen number/total pollen number.
1.3 the evaluation of artificial sexual polyploidization hybridization and filial generation
With tetraploid male sterile is maternal, and the dliploid material that contains the 2n andro gamete with colchicine-induced is a male parent, and reaching untreated is that male parent is carried out the hybridization of artificial flower bud phase respectively to impinging upon the florescence, joins 30 strains altogether, and the 3rd day repeats to pollinate 1 time.Gather in the crops seed mid-May, sowing on September 15 father, female parent and F
1Identify.Morphology, anatomy are identified the method that surpasses with reference to Zhang Zhen, and (Zhang Zhen is super, Zhang Shuning, Zhang Wei, Zhang Hongliang, the inducing and Methods of Ploidy Identification of infertile tetraploid Chinese cabbage, the northwest Botany Gazette, 2007,27 (1): 0028-0032), the investigation morphological indexes has that leaf, leaf look, leaf are thick, plant shape, floral organ, angle fruit etc., the anatomy index has pore, pollen grain, and the The data SPSS software that obtains is added up.Chromosome directly identify method with reference to Zhang Hongmei (Zhang Hongmei, Zhang Shuning, Kong Yane, Zhang Lili utilizes the tender gynoecium of broccoli children to carry out chromosome karyotype analysis, gardening journal, 2009,36 (5): 727-730).
2 results and analysis
2.1 colchicine-induced 2n gamete effect
Adding up (table 1) according to variable concentrations and number of processes shows, 0.05%～0.20% colchicine all can make total variation frequency of pollen (12.06%～24.92%) and 2n gamete frequency (9.52%～20.20%) improve, but the flower survival rate descends (98.6%～70.0%).With concentration 0.20% handle 1 time induce effect best, the flower survival rate is 86.0%, total variation frequency is 24.92%, the 2n gamete frequency reaches 20.20%, is 22 times of contrast.
Table 1 colchicine-induced Chinese cabbage 2n gamete effect
Annotate: total pollen number is the pollen grain summation in 10 visuals field (40 * 10), and capitalization and lowercase are represented significant difference on 0.01 and 0.05 level (P＜0.05, P＜0.01) respectively
2.2 the 2n pollen grain of colchicine-induced and n pollen grain are relatively
See Table 2, compare difference extremely significantly (Fig. 2) with little pollen grain (n) through its big pollen grain (2n) size of material that colchicine is handled, and greatly between pollen grain or little pollen grain differences not remarkable; The n pollen grain that its big pollen grain of material of handling through colchicine extremely significantly contrasts greater than dliploid; And handle 1 time big pollen grain width significantly greater than the tetraploid contrast except that 0.20% colchicine, and the big pollen grain of other processing contrasts no significant difference with tetraploid, and both pollen grains all are blunt ellipse; The n pollen grain of little pollen grain and dliploid contrast does not have significant difference, all ovalize (Fig. 1).Big pollen grain average length is that 165.27 μ m width are 103.72 μ m.The big pollen grain of as seen inducing is the 2n pollen grain.
Table 2 colchicine is handled big pollen (2n) and is compared with little pollen (n)
Annotate: capitalization and lowercase are represented significant difference on 0.01 and 0.05 level (P＜0.05, P＜0.01) respectively
2.3 sexual polyploidization hybridization father and mother's basis and F
1The comparison of pore, floral organ, fruit
By Fig. 4～6, Figure 10～11 as seen, the F of sexual polyploidization hybridization (dliploid through colchicine-induced is a male parent) acquisition
1(tetraploid male sterile line * induce dliploid) compared with dliploid contrast male parent, floral organ shows huge property, the petal color burn, shape becomes circle, longly and wide increase by 82.61% and 103.28% respectively, pore length and widely increase by 93.95% and 71.07% respectively, the angle is really long, horizontal stroke is wide, rib is wide and beak is long increases by 45.33%, 63.33%, 110.00% and 132.14% respectively, but stomatal frequency and the average seed number of single angle fruit reduce 49.58% and 93.29%, and above proterties difference all reaches extremely remarkable; Compare F with the maternal contrast of tetraploid
1Remove single angle average seed number of fruit and reduce 90.20%, outside difference was extremely remarkable, other proterties did not have significant difference.This shows F
1Have tetraploid floral organ and angle fruit huge property (Fig. 6,7), feature that ripening rate is low.
2.4 sexual polyploidization hybridization father and mother's basis and F
1The agronomy proterties relatively
Fig. 8,9 and 11 shows, the F that sexual polyploidization hybridization obtains
1The plant strain growth gesture is strong, but poor growth, blade subcircular (dliploid is long oval), leaf-shrinkage, vein is clear, and width of blade, thickness all contrast greater than the dliploid male parent.Dish is pluckings, individual plant quality, plant height, degree of development, the number of blade, leaf are wide, ten leaf gross masses, leaf length, petiole quality, handle are long, wide, the ten leaf gross thickness of handle and handle thickness rate dliploid increase by 33.81%, 116.95%, 55.68%, 41.54%, 46.15%, 13.43%, 32.18%, 19.81%, 15.91%, 87.24%, 27.55%, 129.79% and 97.72% respectively down, and difference is extremely remarkable; Compare no significant difference with the maternal contrast of tetraploid.This illustrates F
1Has the tetraploid feature.
2.5F
1The root tip chromosomes number is identified
The F of artificial sexual polyploidization hybridization
1Root-tip cells chromosome number mitosis metaphase is 2n=4x=40 (Fig. 3), proves tetraploid.
The F that mentions in the present patent application file
1The resulting infertile tetraploid Chinese cabbage F of the maternal sexual polyploidization breeding of dliploid that refers to that all the tetraploid male sterile line is maternal and induce
1Generation.
Claims (7)
1. the breeding method of an infertile tetraploid Chinese cabbage is characterized in that: use colchicine solution to handle bud at dliploid Chinese cabbage peduncle-growing period for rapeseed and induce generation 2n andro gamete; With tetraploid male sterile is maternal, and the dliploid that contains the 2n andro gamete that obtains with described colchicine-induced is that male parent is carried out artificial sexual polyploidization crossbreeding at the florescence, obtains F
1Be described infertile tetraploid Chinese cabbage for tetraploidy Chinese cabbage.
2. the breeding method of infertile tetraploid Chinese cabbage according to claim 1 is characterized in that described method specifically comprises following steps:
1) inducing of 2n andro gamete: the colchicine solution concentration that mutagenesis dliploid Chinese cabbage produces 2n pollen is 0.05% (g/ml, Hereinafter the same), 0.10% and 0.20%, handle bud at dliploid Chinese cabbage peduncle-growing period for rapeseed, twice of every day, bud size 2.5～3.5mm, adopt micro-injection, each bud injection 5～10 μ L handles once every 6h, and each concentration is handled 2 times, with the plant of being untreated is contrast, bagging is isolated then, and the 2n pollen characteristic is: oval, average length are 165.27 microns, mean breadth is 103.72 microns, and chromosome number is 2x=20;
2) artificial sexual polyploidization hybridization: with tetraploid male sterile is maternal, the dliploid that contains the 2n gamete with colchicine-induced is a male parent, and reaching untreated is that male parent is carried out the hybridization of artificial flower bud phase respectively to impinging upon the florescence, joins 30 strains altogether, the 3rd day repeats to pollinate 1 time, early summer results F
1The tetraploid seed.
3. the breeding method of infertile tetraploid Chinese cabbage according to claim 2, it is characterized in that described colchicine solution handle the time of bud be spring dliploid Chinese cabbage peduncle-growing period for rapeseed 8:00-9:00 in morning and afternoon 2:00-3:00, treatment temperature is respectively 20 ℃-25 ℃ and be not less than 18 ℃.
4. the breeding method of infertile tetraploid Chinese cabbage according to claim 1 and 2 is characterized in that described infertile tetraploid Chinese cabbage F through colchicine mutagenesis 2n pollen and sexual polyploidization hybridization acquisition
1Be white vernalization type in April, plant type is uprightly with a tight waist, 28～31 centimetres of plant heights, 28～34 centimetres of degree of development, the blade subcircular, yellow green, petiole white, flat, individual plant weighs 0.5～0.6 kilogram, compare with dliploid contrast male parent and to show huge property, F1 plant strain growth gesture is strong, the blade subcircular, leaf-shrinkage, vein is clear, dish is pluckings, the individual plant quality, plant height, degree of development, the number of blade, blade is wide, ten leaf gross masses, blade is long, the petiole quality, petiole is long, petiole is wide, ten leaf gross thickness and petiole thickness rate dliploid increase by 33.81% respectively, 116.95%, 55.68%, 41.54%, 46.15%, 13.43%, 32.18%, 19.81%, 15.91%, 87.24%, 27.55%, 129.79% and 97.72%, the petal color burn, shape becomes circle, longly and wide increase by 82.61% and 103.28% respectively, pore length and widely increase by 93.95% and 71.07% respectively, the angle is really long, horizontal wide, increase by 45.33% respectively with beak is long, 63.33%, with 132.14%, but stomatal frequency reduces 49.58%, and above shape difference reaches utmost point significance level; Through cytological Identification, this sexual its somatic chromosome number of polyploidization tetraploid is 2n=4x=40, and dliploid male parent somatic chromosome number is 2n=2x=20.
5. the breeding method of infertile tetraploid Chinese cabbage according to claim 1 and 2 is characterized in that the tetraploid male sterile line of described tetraploid male sterile line selection according to Chinese patent ZL03113283.9 preparation.
6. the breeding method of infertile tetraploid Chinese cabbage according to claim 1 and 2 is characterized in that described dliploid Chinese cabbage selection dliploid Chinese cabbage " brilliant white leaf ".
7. infertile tetraploid Chinese cabbage kind of utilizing the described breeding method of claim 1 to obtain, it is characterized in that the infertile tetraploid Chinese cabbage by this kind is a white vernalization type in April, plant type is uprightly with a tight waist, 28～31 centimetres of plant heights, 28～34 centimetres of degree of development, the blade subcircular, yellow green, petiole white, flat, individual plant weighs 0.5～0.6 kilogram, compare with dliploid contrast male parent and to show huge property, infertile tetraploid Chinese cabbage plant strain growth gesture of the present invention is strong, the blade subcircular, leaf-shrinkage, vein is clear, dish is pluckings, the individual plant quality, plant height, degree of development, the number of blade, blade is wide, ten leaf gross masses, blade is long, the petiole quality, petiole is long, petiole is wide, ten leaf gross thickness and petiole thickness rate dliploid increase by 33.81% respectively, 116.95%, 55.68%, 41.54%, 46.15%, 13.43%, 32.18%, 19.81%, 15.91%, 87.24%, 27.55%, 129.79% and 97.72%, the petal color burn, shape becomes circle, longly and wide increase by 82.61% and 103.28% respectively, pore length and widely increase by 93.95% and 71.07% respectively, the angle is really long, horizontal wide, increase by 45.33% respectively with beak is long, 63.33%, with 132.14%, but stomatal frequency reduces 49.58%, and above shape difference reaches utmost point significance level; Through cytological Identification, this infertile tetraploid Chinese cabbage somatic chromosome number is 2n=4x=40.
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| CN104067931A (en) * | 2014-06-16 | 2014-10-01 | 江苏农林职业技术学院 | Sexual polyploidization breeding method for tetraploid petunias |
| CN108476979A (en) * | 2018-03-13 | 2018-09-04 | 南京理想农业科技有限公司 | A kind of Chinese cabbage tetraploid breeding method |
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| CN113940276A (en) * | 2021-10-20 | 2022-01-18 | 河北省农林科学院经济作物研究所 | Method for creating diploid germplasm resource by using tetraploid Chinese cabbages |
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| CN1077333A (en) * | 1993-01-15 | 1993-10-20 | 南京农业大学 | A kind of breeding method of polyploid cabbage |
| CN1443442A (en) * | 2003-04-25 | 2003-09-24 | 南京农业大学 | Breeding method of tetraploid loose-leaved Chinese cabbage cytoplasm male sterility line |
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2010
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1077333A (en) * | 1993-01-15 | 1993-10-20 | 南京农业大学 | A kind of breeding method of polyploid cabbage |
| CN1443442A (en) * | 2003-04-25 | 2003-09-24 | 南京农业大学 | Breeding method of tetraploid loose-leaved Chinese cabbage cytoplasm male sterility line |
Non-Patent Citations (3)
| Title |
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| 《云南农业大学学报》 20070731 肖亚琼等 鹤望兰2n配子诱导研究初报 第476页左栏第25-30行，第476页右栏第6-7行，第479页左栏4结论部分的倒数第1-4行，摘要第1-6行 1-3，5-6 第22卷, 第4期 2 * |
| 《华北农学报》 19981231 刘学岷等 2n配子在大白菜育种上的应用 第102页摘要 1-3,5-6 第13卷, 第2期 2 * |
| 《西北植物学报》 20071231 张振超等 四倍体不结球白菜的诱导及染色体倍性鉴定 0028－0032 1-7 第27卷, 第1期 2 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103749291A (en) * | 2013-10-21 | 2014-04-30 | 广西壮族自治区农业科学院玉米研究所 | Method for doubling chromosome complement of maize haploid plant |
| CN103749291B (en) * | 2013-10-21 | 2016-04-13 | 广西壮族自治区农业科学院玉米研究所 | A kind of method that corn Chromosomes of Haploid group doubles |
| CN104067931A (en) * | 2014-06-16 | 2014-10-01 | 江苏农林职业技术学院 | Sexual polyploidization breeding method for tetraploid petunias |
| CN108476979A (en) * | 2018-03-13 | 2018-09-04 | 南京理想农业科技有限公司 | A kind of Chinese cabbage tetraploid breeding method |
| CN108476979B (en) * | 2018-03-13 | 2020-04-10 | 南京理想农业科技有限公司 | Breeding method for tetraploid of non-heading Chinese cabbage |
| CN113396818A (en) * | 2021-06-23 | 2021-09-17 | 中国水稻研究所 | Plant breeding method utilizing polyploid heterosis |
| WO2022267794A1 (en) * | 2021-06-23 | 2022-12-29 | 中国水稻研究所 | Plant breeding method using polyploid heterosis |
| CN113940276A (en) * | 2021-10-20 | 2022-01-18 | 河北省农林科学院经济作物研究所 | Method for creating diploid germplasm resource by using tetraploid Chinese cabbages |
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| CN101946696B (en) | 2012-10-03 |
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