CN101940317A - Health food for improving immunity of human bodies and preparation method thereof - Google Patents
Health food for improving immunity of human bodies and preparation method thereof Download PDFInfo
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- CN101940317A CN101940317A CN2010101321552A CN201010132155A CN101940317A CN 101940317 A CN101940317 A CN 101940317A CN 2010101321552 A CN2010101321552 A CN 2010101321552A CN 201010132155 A CN201010132155 A CN 201010132155A CN 101940317 A CN101940317 A CN 101940317A
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Abstract
The invention discloses a health food for improving the immunity of human bodies and a preparation method thereof. The health food comprises the following components by weight percent: 40-50% of rapeseed pollen, 10-25% of grape seed extract, 5-20% of vitamin E, 20-35% of starch and 0-1% of magnesium stearate. The preparation method of the health food comprises the following steps: taking and smashing the rapeseed pollen and sieving; taking the grape seed extract, the vitamin E, the starch and the magnesium stearate, mixing and filling into a capsule. In the health food of the invention, the rapeseed pollen, the grape seed extract and the vitamin E are taken as raw materials, the formula preparing proportion is scientific; and the health food of the invention has no toxic or side effect and is suitable for people with low immunity at each age.
Description
Technical field
The present invention relates to a kind of health food and preparation method thereof, specifically is a kind of health food that improves body immunity and preparation method thereof.
Background technology
Human immune system such as same well-trained crack troops invade the harmful substance of human body and discern, encircle and suppress, and in time remove cell or tissue fragment dead in the metabolism, to keep the balance and stability of organismic internal environment for the external world.But along with the quickening of social rhythm, the increase of life competitive pressure and the radiation interference of various electronic equipments, dysphoria, overwork, insomnia, irregular, the underfed state of daily life have appearred in many people.If body is in this state for a long time, can cause fluctuating widely of interior antibody of body and growth factor secretory volume, and the instability of the interior immunoglobulin content of body, immunity of organisms is descended.Therefore, abundant exercise and logical diet play an important role to the immunity that improves human body.People can reach the purpose of body-building by various motions; The diet aspect, problems can not finely solve all the time because meal time urgent, food collocation is unreasonable etc., therefore are necessary to select health food that the demand of body to nutrition is provided.At present, it is quite a few to improve the body immunity health food, regulates the human immune system but can really play, and the health food that improves immunity is still relatively deficienter.
Summary of the invention
Goal of the invention: the present invention is directed to the prior art deficiency, a kind of health food that improves body immunity is provided.
Another object of the present invention provides the preparation method of above-mentioned health food.
Technical scheme: a kind of health food that improves body immunity comprises following components in weight percentage: 40～50% rape pollen, 10～25% grape seed extract, 5～20% vitamin E, 20～35 starch, 0～1% dolomol.
Wherein, the extraction step of described grape seed extract is as follows:
(1) grape pip is extracted and filters with 70% alcohol reflux;
(2) filtrate is concentrated into nothing alcohol flavor, leaves standstill;
(3) get the supernatant liquor polyamide column chromatography;
(4) with chromatographic solution elder generation water wash-out, use 70% ethanol wash-out once more then;
(5) get eluent and concentrate, the concentrate drying.
Prepare the method for described health food, may further comprise the steps:
(1) gets rape pollen and pulverize, sieve;
(2) getting grape seed extract, vitamin E, starch and dolomol sieves respectively;
(3) rape pollen after will sieving, grape seed extract, vitamin E, starch and dolomol mix;
(4) mixed mixed material is incapsulated.
Wherein, step (1) and step (2) are preferentially selected 60 mesh sieves for use.
Wherein, the incorporation time of step (3) is best with 30min.
Wherein, the mixed material that incapsulates is advisable with 0.35g.
Rape pollen is the article that can be used for health food.Pollen, is widely used in fields such as nutrition, health care, medical treatment owing to contain multiple nutritional components as a kind of internationally recognized pure natural complete nutrition product.Pollen polysaccharide in the rape flower can obviously improve nonspecific cellular immunity of body and specific humoral immunity, improve the kill capability of body NK cell to tumour cell, simultaneously can strengthen humoral immunity and cellular immune function, nonspecific immunity function, humoral immune function, cellular immune function are all had certain humidification.
Grape is a bread and cheese, according to the 63 regulation of health food registration management way, can be used as the raw material of health food.The proanthocyanidin composition that contains in the grape pip can strengthen energy, strengthens immunity; Grape seed polyphenols has certain immunoloregulation function.
Vitamin E is the important liposoluble vitamin that has extensive physiological function in the human body, except promoting reproduction, keeps central nervous system, cardiovascular system and unifies outside the function of skeletal muscle, also has the effect of immunomodulator.Vitamin E is by strengthening humoral immunity, improve cell-mediated immune responses, influence phagocytic function etc. and act on and influence immune status.It is to avoid oxidation by protection cell membrane and organelle film to destroy on the one hand that vitamin E improves the animal immune ability, keeps the complete of cell and organelle with stable, to guarantee the normal function of cell; Be that the adjustable ganglion cell of vitamin E regulates the synthetic of element, prostaglandin, haemoglutinin and promoting growth of cell element on the other hand.
Beneficial effect: it is raw material that the present invention adopts rape pollen, grape pip and vitamin E, and the side's of going into ratio science is without any side effects, is fit to the crowd of each age level hypoimmunity.
The specific embodiment
Embodiment 1:
Get 40% rape pollen (unit is a percetage by weight, and is together following) pulverizing, 60 mesh sieves excessively; Get 21.5% grape seed extract, 5.5% vitamin E, 32.5% starch and 0.5% dolomol and cross 60 mesh sieves respectively; With the rape pollen after sieving, grape seed extract, vitamin E, starch and dolomol mixing 30min, and incapsulate every 0.35g.
Embodiment 2:
Get 44.5% rape pollen and pulverize, cross 60 mesh sieves; Get 19.5% grape seed extract, 10.5% vitamin E, 25% starch and 0.5% dolomol and cross 60 mesh sieves respectively; With the rape pollen after sieving, grape seed extract, vitamin E, starch and dolomol mixing 30min, and incapsulate every 0.35g.
Embodiment 3:
Get 47.5% rape pollen and pulverize, cross 60 mesh sieves; Get 14.5% grape seed extract, 9.5% vitamin E, 28% starch and 0.5% dolomol and cross 60 mesh sieves respectively; With the rape pollen after sieving, grape seed extract, vitamin E, starch and dolomol mixing 30min, and incapsulate every 0.35g.
Embodiment 4:
Get 49.5% rape pollen and pulverize, cross 60 mesh sieves; Get 12% grape seed extract, 17.5% vitamin E, 20.5% starch and 0.5% dolomol and cross 60 mesh sieves respectively; With the rape pollen after sieving, grape seed extract, vitamin E, starch and dolomol mixing 30min, and incapsulate every 0.35g.
Embodiment 5:
Get 55% rape pollen and pulverize, cross 60 mesh sieves; Get 11.5% grape seed extract, 14.5% vitamin E, 28.5% starch and 0.5% dolomol and cross 60 mesh sieves respectively; With the rape pollen after sieving, grape seed extract, vitamin E, starch and dolomol mixing 30min, and incapsulate every 0.35g.
Embodiment 6:
The present invention improves the immunity function experimental study
1. material
1.1 sample: provide by the Wuxi City health bio tech ltd of being bestowed by heaven.Adult's recommended amounts every day is 2100mg/60kg.BW, is tried thing with the preparation of distillation water as solvent during test.
1.2 reagent, experiment equipment and animal
1.2.1 reagent
SRBC (sheep red blood cell (SRBC)), Hank ' s liquid, DNFB (2,4 dinitrofluorobenzene), S
ABuffer solution, agarose, india ink, Na
2CO
3(sodium carbonate), RPMI1640 nutrient solution, chicken erythrocyte suspension, physiological saline, YAC-1 cell.Above reagent is provided by the disease prevention and control center, Sichuan Province.
1.2.2 experiment equipment
100 μ l micro syringes, CO2gas incubator, electronic analytical balance, 723 spectrophotometers, centrifuge, operating theater instruments
1.2.3 animal used as test
Reach 160 of the Kunming mouses that large bio tech ltd provides by Chengdu, female entirely, body weight 18-22g, production licence number are SCXK (river) 2008-24SPF level; The experimental animal room is a barrier system, and occupancy permit number is real moving pipe SYXK (river) 2008-043 in river.Temperature 20-25 ℃, relative humidity 40～70%.
2. experimental technique
2.1 dosage is selected
Tried thing and established 350mg/kg.BW, 700mg/kg.BW, three dosage groups of 1050mg/kg.BW (be equivalent to respectively human body recommended intake 10 times, 20 times, 30 times), take by weighing 4.38g, 8.75g respectively, 13.13g is tried the thing adding distil water to the 250ml mixing, stored refrigerated, use up again and join, other establishes the distilled water negative control group, every group of 40 animals.Be divided into immune one group, two groups, three groups, four groups, wherein one group is used for Turnover of Mouse Peritoneal Macrophages and engulfs chicken red blood cell experiment; Two groups are used for NK cytoactive mensuration and lymphocyte transformation experiment; Three groups are used for antibody-producting cell experiment, serum hemolysin mensuration and delayed allergy; Four groups are used for mouse carbon and clean up experiment.Mouse is pressed per os of 20mg/kg.BW body weight and irritates stomach, gives 30 days continuously, claims body weight weekly one time, and it is long-pending to adjust the filling body of stomach.
2.2 experimental procedure
2.2.1 the clearance test of mouse carbon: the continuous irrigation stomach is after 30 days, in the india ink of mouse tail vein injection with 5 times of physiological saline dilutions, timing immediately after pressing 0.1ml/10g prepared Chinese ink and injecting is after injecting prepared Chinese ink the 2nd, 10min gets blood 20 μ l from the angular vein clump respectively, is added to 2mlNa
2CO
3In the solution, with Na
2CO
3Solution is made blank, measures OD value at the 600nm place with 723 spectrophotometers.Put to death mouse after getting blood, get liver, spleen is weighed, calculate phagocytic index.
2.2.2 Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell experiment (half intracorporal method): the continuous irrigation stomach is after 30 days, the chicken erythrocyte suspension 1ml of every mouse peritoneal injection 20%, after 30 minutes, the cervical vertebra dislocation is put to death, be fixed on the mouse plate, abdominal skin is cut off in the center, inject physiological saline 2ml through the abdominal cavity, rotated the mouse plate 1 minute, sucking-off abdominal cavity washing lotion 1ml, mean droplet is on 2 slides, put 37 ℃ in wet box 30 minutes, and took out in the physiological saline rinsing, dry, fixing, 4%GiemsaPBS dyeing 3 minutes, the distilled water rinsing is dried, microscopy.Calculate phagocytic percentage and phagocytic index.
2.2.3 delayed allergy (DTH) (the sufficient sole of the foot thickens method): irritate stomach after 30 days, inject 2% hematocrit SRBC (the every mouse of 0.2ml/) sensitization after 4 days to mouse peritoneal, measure left back sufficient sole of the foot thickness, and then measuring point hypodermic injection 20% (v/v) SRBC (the every mouse of 20 μ l/), 24h measures left back sufficient sole of the foot thickness in the injection back, same position is measured three times, averages, and represents the degree of DTH with sufficient sole of the foot thickness difference (swelling degree of the paw) before and after attacking.
2.2.4ConA the mouse lymphocyte conversion test (mtt assay) of inducing: at first carry out the preparation of splenocyte suspension.Cell concentration is adjusted into 3 * 10
6Individual/ml, then cell suspension is divided two holes to add in 24 well culture plates, every hole 1ml, a hole adds 75 μ lConA liquid, and another hole compares, and puts 5%CO
2Incubator is cultivated 72h for 37 ℃.Cultivate and finish preceding 4h, every hole is inhaled supernatant 0.7ml gently and is added the RPMI1640 nutrient solution that 0.7ml does not contain calf serum, add MTT (5mg/ml) 50 μ l/ holes simultaneously and continue to cultivate 4h, after cultivating end, every hole adds 1ml acid isopropyl alcohol, purple crystal is dissolved fully, then every hole liquid is moved in the cuvette, with 723 spectrophotometers in 570nm wavelength place's colorimetric estimation OD value.
2.2.5 antibody-producting cell detects (Jernr improves slide method): the continuous irrigation stomach is after 30 days, every mouse peritoneal is injected 2% hematocrit SRBC0.2ml, the mouse cervical vertebra dislocation of immunity after 5 days half put to death, take out spleen and be placed in the plate that fills Hank ' s liquid, make splenocyte suspension.Agarose is made into 1% aqueous solution, and 30min is boiled in water-bath, mix with the double Hank ' s of equivalent liquid, and the packing small test tube, every pipe 0.5ml adds 10% again and (uses S in pipe
AThe buffer solution preparation) hematocrit SRBC50 μ l, each 20 μ l of splenocyte suspension make two parallel samples, rapidly behind the mixing, be poured on the agarose thin layer slide, treat that agar solidifies after, the slide level buckled be placed on the horse, put into CO2gas incubator and hatch 1.5h, then complement is joined in the slide frame groove, continue incubation 1.5h, counting hemolysis plaque number.
2.2.6 serum hemolysin is measured (blood clotting method): at the continuous irrigation stomach after 30 days, every mouse peritoneal injection 2%SRBC0.2ml immunity, continue to irritate stomach after 5 days, extract eyeball and get blood in centrifuge tube, place 1h, peel off, the centrifugal 10min of 2000r/min collects serum, with physiological saline serum is made doubling dilution, every part of dilution 12 holes place blood-coagulation-board with the dilution serum of difference, every hole 100 μ l, add 0.5%SRBC suspension 100 μ l again, mixing is put observed result behind wet 37 ℃ of 3h of box, writes down the aggegation degree in every hole.The calculating antibody product.
2.2.7NK cytoactive is measured (lactate dehydrogenase L DH determination method): the continuous irrigation stomach is after 30 days, and animal is put to death in the cervical vertebra dislocation, takes out spleen, tears up, cross 200 eye mesh screens after, use Hank ' s liquid to wash 3 times, be made into 2 * 10 with complete RPMI1640 nutrient solution
7Individual/the ml cell suspension.The cell suspension of each mouse is got 300 μ l divide 3 holes to place 96 well culture plates, every hole 100 μ l, every hole adds target cell (YAC-1 cell, 4 * 10
5Individual/ml) 100 μ l, do target cell nature release aperture (target cell 100 μ l+ nutrient solutions 100 μ l) and each 8 hole of maximum release aperture (target cell 100 μ l+2.5%Triton100 μ l) simultaneously, 37 ℃ of 5%CO
2Cultivated 4 hours, and took out the centrifugal 5min of 1500r/min.Each hole supernatant 100 μ l is placed another culture plate, and every hole adds 100 μ l matrix liquid again, adds the HCL30 μ l cessation reaction of 1mol/L after 10 minutes, measures the OD value at the 490nm place, calculates NK cytoactive rate.
3. test data is added up
Test data adopts SPSS10.0 for Windows software kit to handle.The data of control group and dosage group are through the variance test of homogeneity, and variance is neat, carry out variance analysis,, then compare in twos with the Dunnett method less than 0.05 as the P value; If heterogeneity of variance then carries out data transaction, and is still uneven, use rank test instead, less than 0.05, then use Dunnett ' sT3 method to compare (P＞0.05 is non-significant difference, and P＜0.05 is a significant difference) in twos as the P value.
4. result
4.1 the present invention sees Table 1-4 to the influence of mouse body weight.
Table 1 is respectively organized the initial body weight of mouse
Table 2 is respectively organized the body weight in mid-term of mouse
Table 3 is respectively organized the end body weight of mouse
Table 4 the present invention is to the influence of mouse weight gain
By table 1-4 as seen, the initial body weight of one group, immune two groups of the present invention immunity, immune three groups and immune four groups of mouse is compared with negative control group, through the variance test of homogeneity, variance neat (P＞0.05), and The results of analysis of variance (P＞0.05) illustrates that the initial body weight of respectively organizing between mouse and the negative control group is balanced.Three dosage group mouse test mid-terms, the body weight in latter stage and the growths of duration of test mouse body weight are compared with negative control group, and learn by statistics and handle, there was no significant difference (P＞0.05), i.e. the present invention does not have influence to the body weight gain of mouse.
4.2 the present invention is to the influence of mouse monokaryon-macrophage phagocytic function
Table 5 the present invention cleans up the influence of function to mouse monokaryon-macrophage carbon
By table 5 as seen, the continuous irrigation stomach is after 30 days, and the carbon of three dosage group mouse is cleaned up ability and compared with negative control group, learns by statistics and handles there was no significant difference (P＞0.05).
Table 6 the present invention engulfs the influence of chicken red blood cell ability to mouse macrophage
By table 6 as seen, the continuous irrigation stomach is after 30 days, and the phagocytic rate of three dosage treated animals is compared with negative control group with phagocytic index, learns by statistics and handles there was no significant difference (P＞0.05).
By table 5, table 6 as seen, the present invention is to mouse monokaryon-macrophage phagocytic function test feminine gender as a result.
4.3 the present invention is to the influence of mouse cell immunity
Table 7 the present invention is to the influence of mouse delayed allergy (DTH)
By table 7 as seen, the continuous irrigation stomach is after 30 days, and the swelling degree of the paw of three dosage group mouse is compared with negative control group, learns by statistics and handles, and middle and high dosage group has significant difference (P＜0.01, P＜0.05)
The influence of the mouse lymphocyte conversion test that table 8 the present invention induces ConA
By table 8 as seen, continuous irrigation stomach mouse is after 30 days, and the lymphopoiesis ability of three dosage group mouse is compared with negative control, learns by statistics and handles there was no significant difference (P＞0.05).
Visible the present invention is positive to mouse cell immunity test result by table 7, table 8.
4.4 the present invention is to the influence of mouse humoral immune
Table 9 the present invention is to the influence of mouse antibodies cellulation
By table 9 as seen, the continuous irrigation stomach is after 30 days, and the hemolysis plaque number of three dosage group mouse is compared with negative control, learns by statistics and handles difference that there are no significant (P＞0.05).
Table 10 the present invention is to the influence of mice serum hemolysin
By table 10 as seen, the continuous irrigation stomach is after 30 days, and the antibody product of three dosage group mouse is compared with negative control group, learns by statistics and handles, and low, high dose group has utmost point significant difference (P＜0.01, P＜0.01)
By table 9, table 10 as seen, the present invention is positive to the mouse humoral immune result of the test.
4.5 the present invention is to the influence of NK cells in mice activity
Table 11 the present invention is to the influence of NK cells in mice activity
By table 11 as seen, the continuous irrigation stomach is after 30 days, and three dosage group NK cells in mice activity are compared with negative control, learns by statistics and handles there was no significant difference (P＞0.05).The present invention shows negative to the NK cytoactive result of the test of mouse.
5. sum up
Continuous irrigation stomach mouse of the present invention did not have obvious influence to the mouse body weight after 30 days; To monokaryon-macrophage phagocytic function test of mouse feminine gender as a result; To the NK test cell line of mouse feminine gender as a result; To the test for celluar immunity of mouse, swelling degree of the paw of three dosage group mouse and negative control group compare, and middle and high dosage group difference has conspicuousness (P＜0.01, P＜0.05), the result of the test positive; To the test for humoral immunity of mouse, antibody product of three dosage group mouse and negative control group compare, and low, high dose group difference all has conspicuousness (P＜0.01, P＜0.01), the result of the test positive.
Can judge that according to " health food check and assessment technique standard " version in 2003 the present invention has the function that improves immunity.
Claims (5)
1. a health food that improves body immunity is characterized in that it comprises following components in weight percentage: 40～50% rape pollen, 10～25% grape seed extract, 5～20% vitamin E, 20～35 starch, 0～1% dolomol.
2. a kind of health food that improves body immunity according to claim 1 is characterized in that the extraction step of described grape seed extract is as follows:
(1) grape pip is extracted and filters with 70% alcohol reflux;
(2) filtrate is concentrated into nothing alcohol flavor, leaves standstill;
(3) get the supernatant liquor polyamide column chromatography;
(4) with chromatographic solution elder generation water wash-out, use 70% ethanol wash-out once more then;
(5) get eluent and concentrate, the concentrate drying.
3. prepare the described a kind of method that improves the health food of body immunity of claim 1, it is characterized in that may further comprise the steps:
(1) gets rape pollen and pulverize, sieve;
(2) getting grape seed extract, vitamin E, starch and dolomol sieves respectively;
(3) rape pollen after will sieving, grape seed extract, vitamin E, starch and dolomol mix;
(4) mixed mixed material is incapsulated.
4. a kind of method that improves the health food of body immunity of preparation according to claim 3 is characterized in that step (1) and step (2) select 60 mesh sieves for use.
5. a kind of method that improves the health food of body immunity of preparation according to claim 3, the incorporation time that it is characterized in that step (3) is 30min.
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|---|---|---|---|
| CN2010101321552A CN101940317B (en) | 2010-03-25 | 2010-03-25 | Health food for improving immunity of human bodies and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101321552A CN101940317B (en) | 2010-03-25 | 2010-03-25 | Health food for improving immunity of human bodies and preparation method thereof |
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| Publication Number | Publication Date |
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| CN101940317A true CN101940317A (en) | 2011-01-12 |
| CN101940317B CN101940317B (en) | 2012-07-25 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105919117A (en) * | 2016-04-30 | 2016-09-07 | 莆田市山海天农业发展有限公司 | Preparation method of functional kelp food |
| CN106039258A (en) * | 2016-06-29 | 2016-10-26 | 中国人民解放军第三军医大学第附属医院 | Composition used for enhancing immunity and application thereof |
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| CN1478505A (en) * | 2002-08-30 | 2004-03-03 | 宁波大红鹰生物工程股份有限公司 | Capsule for delayed senility and its manufacturing method |
| CN1565542A (en) * | 2003-06-24 | 2005-01-19 | 中国科学院西北高原生物研究所 | Blood pressure reducing and blood fat removing healthy compositions |
| US20080057157A1 (en) * | 2006-08-31 | 2008-03-06 | Helbert Almeida | Puffed Cracker-Like Food Products And Method Of Making |
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2010
- 2010-03-25 CN CN2010101321552A patent/CN101940317B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1478505A (en) * | 2002-08-30 | 2004-03-03 | 宁波大红鹰生物工程股份有限公司 | Capsule for delayed senility and its manufacturing method |
| CN1565542A (en) * | 2003-06-24 | 2005-01-19 | 中国科学院西北高原生物研究所 | Blood pressure reducing and blood fat removing healthy compositions |
| US20080057157A1 (en) * | 2006-08-31 | 2008-03-06 | Helbert Almeida | Puffed Cracker-Like Food Products And Method Of Making |
Non-Patent Citations (1)
| Title |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105919117A (en) * | 2016-04-30 | 2016-09-07 | 莆田市山海天农业发展有限公司 | Preparation method of functional kelp food |
| CN106039258A (en) * | 2016-06-29 | 2016-10-26 | 中国人民解放军第三军医大学第附属医院 | Composition used for enhancing immunity and application thereof |
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|---|---|
| CN101940317B (en) | 2012-07-25 |
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Effective date of registration: 20160413 Address after: The Institute of tea in Jiangsu province Yixing City Road 214204 No. 18 Patentee after: Jiangsu Kingsley Pharmaceutical Co., Ltd. Address before: Huankeyuan Garden Road 214222 Jiangsu city of Yixing province No. 48 Yixing science and Technology Innovation Service Center Room 503 Patentee before: Wuxi City Tiancikang Biotechnology Co., Ltd. |
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Address after: 214204 Jiangsu province Yixing huankeyuan Tea Road No. 18 Patentee after: Jiangsu Kingsley Pharmaceutical Co., Ltd. Address before: The Institute of tea in Jiangsu province Yixing City Road 214204 No. 18 Patentee before: Jiangsu Kingsley Pharmaceutical Co., Ltd. |
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Address after: Huankeyuan Tea Road 214200 Jiangsu city of Yixing province No. 18 Patentee after: Jiangsu Kingsley pharmaceutical Limited by Share Ltd Address before: 214204 Jiangsu province Yixing huankeyuan Tea Road No. 18 Patentee before: Jiangsu Kingsley Pharmaceutical Co., Ltd. |