CN101914575A - Method for producing ethanol fuel by fermenting sweet sorghum stalk concentrated juice by using zymomonas mobilis - Google Patents
Method for producing ethanol fuel by fermenting sweet sorghum stalk concentrated juice by using zymomonas mobilis Download PDFInfo
- Publication number
- CN101914575A CN101914575A CN2010102417251A CN201010241725A CN101914575A CN 101914575 A CN101914575 A CN 101914575A CN 2010102417251 A CN2010102417251 A CN 2010102417251A CN 201010241725 A CN201010241725 A CN 201010241725A CN 101914575 A CN101914575 A CN 101914575A
- Authority
- CN
- China
- Prior art keywords
- zymomonas mobilis
- syrup
- fermentation
- sweet sorghum
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for producing ethanol fuel by fermenting sweet sorghum stalk concentrated juice by using zymomonas mobilis. The method comprises the following steps of: (1) mechanically pressing sweet sorghum stalks to obtain sugar liquid; (2) concentrating the sugar liquid to form sugar juice; (3) adjusting the concentration of the sugar juice to obtain dilute sugar juice of which the mass concentration of total sugar is 5 to 15 percent, adjusting the pH, and adding penicillin into the sugar juice; (4) inoculating zymomonas mobilis seed solution to perform culture; (5) adding the sugar juice obtained in the step (2) into the seed solution, and fermenting the sugar juice; and (6) rectifying the fermentation solution to obtain ethanol, and dehydrating the ethanol to obtain the ethanol fuel. The method has the advantages that: the concentrated sugar juice can be stored in a storage tank without adding preservative so as to prevent the preservative from affecting the growth and fermentation of the zymomonas mobilis; and the fermentation period is short and is less than 52 hours, and the yield of the ethanol is up to over 90 percent.
Description
Technical field
The invention belongs to technical field of bioengineering, relate to a kind of production alcoholic acid method, more specifically relate to a kind of method of producing alcohol fuel with zymomonas mobilis fermenting sweet sorghum stem stalk concentrated juice.
Background technology
Sweet sorghum is high light efficiency C
4Crop, its biological yield is high, and general per hectare can be produced grain 2.25～6t, bright stem stalk 60～75t, and the stem stalk is rich in sugar, and juice content is up to 50%～70%, sugar degree 12%～22%.Sweet sorghum output height; cultivation easily; purposes is extremely wide; contained sugar very easily is converted into ethanol in the stem stalk in addition; but per hectare is the stem stalk about 7875L of producing and ethanol just only, and ethanol is the surrogate of gasoline, only from national energy security; Economic development and environment protection aspect are said, are that raw material development alcohol fuel holds out broad prospects with the sweet sorghum.The test-results of India shows that the alcohol output of per hectare sugarcane is 4000～5000L/, and sweet sorghum can reach 5700-6500L/.The alcohol output in U.S. per hectare sweet sorghum every year reaches 6106L, and be known as sun power the sugarcane of effective transmodulator have only 4680L.
With the sweet sorghum is that its advantage is arranged in the raw material production ethanol process, and its shortcoming is also arranged.Main advantage is biomass through simple processing back just can the direct fermentation utilization, does not need the enzyme processing.Main drawback comprises that seasonality, sugar squeeze the storage with crop.Sweet sorghum harvest season high humidity reaches 70-80%, is the ecotopia that natural microorganisms utilizes carbohydrate in the juice, thereby causes corruption.Even the stem stalk is tied up room temperature storage, available carbohydrate is also corrupt easily.
Zymomonas mobilis is Gram-negative bacteria, is that occurring in nature is unique up to now known with pyruvic carboxylase and ethanol dehydrogenase and unique Enter-Doudoroff (ED) pathways metabolism coupling connection High-efficient Production alcoholic acid microorganism mutually.Compare with traditional microorganism that is used for ethanol fermentation production such as yeast saccharomyces cerevisiae, zymomonas mobilis has following advantage: (1) every metabolism a part glucose only produces a part ATP, therefore the biomass that generates is less, and alcohol output is near theoretical maximum (96%); (2) accretion rate is fast, than high nearly 5 times of yeast; (3) product specificity height, the nutrition of growth needs is simple; (4) the same with yeast saccharomyces cerevisiae, zymomonas mobilis also is the safe bacterial strain of generally acknowledging, the fermentation thalline can be used as animal-feed or fertilizer after simple process.Zymomonas mobilis is energy metabolism glucose, fructose and sucrose only, and the sugar in the juice of sugar grass stalks mainly is these three kinds of sugar, therefore has competitive edge with zymomonas mobilis fermenting sweet sorghum stalk juice.
At present, utilize sweet sorghum stalk produce ethanol research more be to utilize juice of sugar grass stalks or seed to carry out fermentative production with the method for juice, as Chinese patent application number: 200710304648.8 " a kind of methods of utilizing preparing ethyl alcohol from sweet sorghum ", application number: 200510076833.7 " methods of producing alcohol from sugar grass straw juice " etc., in these patent applications in the disclosed juice of sugar grass stalks sugared concentration lower, the alcohol concn of producing is low, and this its economy on industrial production is relatively poor.
Utilize the research of sorgo stalk solid ferment production alcoholic acid also a lot, but have many shortcomings: (1) solid state fermentation volume is big, and the yeast usage quantity is big; (2) fermentation period is long; (3) distillation energy consumption height; (4) produce a large amount of waste residues, promptly enable to do feed, but its water content is big, drying process need consume more multipotency.
Summary of the invention
The objective of the invention is to overcome the lower deficiency of alcohol concn of the juice of sugar grass stalks liquid fermenting production that exists in the prior art, shortcomings such as sweet sorghum stalk solid fermentation fermentation period is long, the yeast usage quantity is big provide a kind of method of producing alcohol fuel with zymomonas mobilis fermenting sweet sorghum stem stalk concentrated juice.
Technical scheme of the present invention is summarized as follows:
With the method that zymomonas mobilis fermenting sweet sorghum stem stalk concentrated juice is produced alcohol fuel, form by following step:
(1) mechanical expression sweet sorghum stalk obtains liquid glucose;
(2) concentrate described liquid glucose and obtain syrup, make that the mass concentration of total reducing sugar reaches 45%-65% in the syrup;
(3) concentration of the syrup of regulating step (2) acquisition, the mass concentration that obtains total reducing sugar is rare syrup of 5%-15%, regulates pH to 3.5-5.0, and adds penicillin, making penicillin concn is 0.1-1000U/ml;
(4) insert the zymomonas mobilis seed liquor, cultivate;
(5) stream adds the syrup that described step (2) obtains, and ferments;
(6) rectifying obtains ethanol, again through dehydration, gets alcohol fuel.
Described step (4) is: inserting the zymomonas mobilis seed liquor, is that the concentration that is cultured to zymomonas mobilis under the condition of 40-500rpm reaches 1.0 * 10 at 30 ± 5 ℃, mixing speed
8More than individual cell/ml.
Described step (5) is: stream adds the syrup that described step (2) obtains, the mass concentration of the speed control that syrup stream adds total reducing sugar in fermented liquid is below 12%, treat in the fermented liquid that the alcoholic acid volumetric concentration is to stop stream at 7%～8% o'clock to add, adopt batch fermentation, semicontinuous fermentation, continuously ferment or have the fermentation mode fermentation of zymomonas mobilis reuse, treat that the alcoholic acid volumetric concentration is 12.0%-14.0% in the fermented liquid, the mass concentration of residual total reducing sugar is below 2.0%, and the mass concentration of residual reducing sugar is 0.2% to stop fermentation when following.
Advantage of the present invention is: spissated syrup need not additionally be added sanitas and just can be stored in hold tank, avoids influencing because of sanitas growth and the fermentation of zymomonas mobilis; Fermentation period weak point＜52h; Ethanol yield height is more than 90%.
The present invention adopts zymomonas mobilis fermenting sweet sorghum stem stalk concentrated juice to carry out fuel ethanol production, and fermentation is divided into two stages, i.e. seed culture stage and fermentation stage.Seed culture adopts the mode of amplifying step by step, saves the seed liquor of setting out; Zymomonas mobilis is a bacterium, and fermenting speed is faster than yeast, and fermentation period is shorter relatively; The present invention adopts liquid submerged fermentation, does not produce the very big solid matter of a large amount of water content, save the energy consumption of dry solids, so energy consumption is relatively low.
Embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
Embodiment 1
With the method that zymomonas mobilis fermenting sweet sorghum stem stalk concentrated juice is produced alcohol fuel, form by following step:
(1) mechanical expression sweet sorghum stalk obtains liquid glucose;
(2) concentrate described liquid glucose and obtain syrup, make in the syrup mass concentration of total reducing sugar reach 50% and be stored in the hold tank and deposit;
(3) add the concentration of the syrup that water regulating step (2) obtains, the mass concentration that obtains total reducing sugar is rare syrup of 10%, regulates pH to 4.0, and adds penicillin, and making penicillin concn is 10U/ml;
(4) inserting the zymomonas mobilis seed liquor, is that the concentration that is cultured to zymomonas mobilis under the condition of 100rpm reaches 1.0 * 10 at 30 ± 5 ℃, mixing speed
8More than individual cell/ml;
(5) stream adds the syrup that described step (2) obtains, the mass concentration of the speed control that syrup stream adds total reducing sugar in fermented liquid is below 10%, treat in the fermented liquid that the alcoholic acid volumetric concentration is to stop stream at 7% o'clock to add, adopt batch fermentation, treat that the alcoholic acid volumetric concentration is more than 13.0% in the fermented liquid, the mass concentration of residual total reducing sugar is below 2.0%, and the mass concentration of residual reducing sugar is 0.2% to stop fermentation when following;
(6) rectifying obtains ethanol, again through dehydration, gets alcohol fuel.The ethanol yield is 92.6%.
Embodiment 2
With the method that zymomonas mobilis fermenting sweet sorghum stem stalk concentrated juice is produced alcohol fuel, form by following step:
(1) mechanical expression sweet sorghum stalk obtains liquid glucose;
(2) concentrate described liquid glucose and obtain syrup, make in the syrup mass concentration of total reducing sugar reach 45% and be stored in the hold tank and deposit;
(3) add the concentration that water is regulated the syrup of regulating step (2) acquisition, the mass concentration that obtains total reducing sugar is rare syrup of 5%, regulates pH to 4.5, and adds penicillin, and making penicillin concn is 1000U/ml;
(4) inserting the zymomonas mobilis seed liquor, is that the concentration that is cultured to zymomonas mobilis under the condition of 100rpm reaches 1.0 * 10 at 30 ± 5 ℃, mixing speed
8Individual cell/ml;
(5) stream adds the syrup that described step (2) obtains, the mass concentration of the speed control that syrup stream adds total reducing sugar in fermented liquid is below 8%, treat in the fermented liquid that the alcoholic acid volumetric concentration is to stop stream at 8% o'clock to add, adopt batch fermentation, treat that the alcoholic acid volumetric concentration is more than 12.0% in the fermented liquid, the mass concentration of residual total reducing sugar is below 2.0%, and the mass concentration of residual reducing sugar is 0.2% to stop fermentation when following;
(6) rectifying obtains ethanol, again through dehydration, gets alcohol fuel.The ethanol yield is 93.7%.
Embodiment 3
With the method that zymomonas mobilis fermenting sweet sorghum stem stalk concentrated juice is produced alcohol fuel, form by following step:
(1) mechanical expression sweet sorghum stalk obtains liquid glucose;
(2) concentrate described liquid glucose and obtain syrup, make in the syrup mass concentration of total reducing sugar reach 65% and be stored in the hold tank and deposit;
(3) add the concentration that water is regulated the syrup of regulating step (2) acquisition, the mass concentration that obtains total reducing sugar is rare syrup of 15%, regulates pH to 3.5, and adds penicillin, and making penicillin concn is 0.1U/ml;
(4) inserting the zymomonas mobilis seed liquor, is that the concentration that is cultured to zymomonas mobilis under the condition of 40rpm reaches 1.0 * 10 at 30 ± 5 ℃, mixing speed
8More than individual cell/ml;
(5) stream adds the syrup that described step (2) obtains, the mass concentration of the speed control that syrup stream adds total reducing sugar in fermented liquid is below 12%, treat in the fermented liquid that the alcoholic acid volumetric concentration is to stop stream at 8% o'clock to add, adopt semicontinuous fermentation, treat that the alcoholic acid volumetric concentration is 14.0% in the fermented liquid, the mass concentration of residual total reducing sugar is below 2.0%, and the mass concentration of residual reducing sugar is 0.2% to stop fermentation when following;
(6) rectifying obtains ethanol, again through dehydration, gets alcohol fuel.The ethanol yield is 91.5%.
Embodiment 4
With the method that zymomonas mobilis fermenting sweet sorghum stem stalk concentrated juice is produced alcohol fuel, form by following step:
(1) mechanical expression sweet sorghum stalk obtains liquid glucose;
(2) concentrate described liquid glucose and obtain syrup, make in the syrup mass concentration of total reducing sugar reach 55% and be stored in the hold tank and deposit;
(3) add the concentration that water is regulated the syrup of regulating step (2) acquisition, the mass concentration that obtains total reducing sugar is rare syrup of 8%, regulates pH to 5.0, and adds penicillin, and making penicillin concn is 100U/ml;
(4) inserting the zymomonas mobilis seed liquor, is that the concentration that is cultured to zymomonas mobilis under the condition of 300rpm reaches 1.0 * 10 at 30 ± 5 ℃, mixing speed
8More than individual cell/ml;
(5) stream adds the syrup that described step (2) obtains, the mass concentration of the speed control that syrup stream adds total reducing sugar in fermented liquid is below 11%, treat in the fermented liquid that the alcoholic acid volumetric concentration is to stop stream at 7% o'clock to add, employing is continuously fermented, treat that the alcoholic acid volumetric concentration is 12.5% in the fermented liquid, the mass concentration of residual total reducing sugar is below 2.0%, and the mass concentration of residual reducing sugar is 0.2% to stop fermentation when following;
(6) rectifying obtains ethanol, again through dehydration, gets alcohol fuel.The ethanol yield is 93.2%.
Embodiment 5
With the method that zymomonas mobilis fermenting sweet sorghum stem stalk concentrated juice is produced alcohol fuel, form by following step:
(1) mechanical expression sweet sorghum stalk obtains liquid glucose;
(2) concentrate described liquid glucose and obtain syrup, make in the syrup mass concentration of total reducing sugar reach 50% and be stored in the hold tank and deposit;
(3) add the concentration that water is regulated the syrup of regulating step (2) acquisition, the mass concentration that obtains total reducing sugar is rare syrup of 10%, regulates pH to 4.5, and adds penicillin, and making penicillin concn is 5U/ml;
(4) inserting the zymomonas mobilis seed liquor, is that the concentration that is cultured to zymomonas mobilis under the condition of 500rpm reaches 1.0 * 10 at 30 ± 5 ℃, mixing speed
8More than individual cell/ml;
(5) stream adds the syrup that described step (2) obtains, the mass concentration of the speed control that syrup stream adds total reducing sugar in fermented liquid is below 12%, treat in the fermented liquid that the alcoholic acid volumetric concentration is to stop stream at 8% o'clock to add, employing has the fermentation mode fermentation of zymomonas mobilis reuse, treat that the alcoholic acid volumetric concentration is 14.0% in the fermented liquid, the mass concentration of residual total reducing sugar is below 2.0%, and the mass concentration of residual reducing sugar is 0.2% to stop fermentation when following;
(6) rectifying obtains ethanol, again through dehydration, gets alcohol fuel.The ethanol yield is 92.4%.
Embodiment 6
With the method that zymomonas mobilis fermenting sweet sorghum stem stalk concentrated juice is produced alcohol fuel, form by following step:
(1) mechanical expression sweet sorghum stalk obtains liquid glucose;
(2) concentrate described liquid glucose and obtain syrup, make in the syrup mass concentration of total reducing sugar reach 52% and be stored in the hold tank and deposit;
(3) add the concentration that water is regulated the syrup of regulating step (2) acquisition, the mass concentration that obtains total reducing sugar is rare syrup of 6%, regulates pH to 4.2, and adds penicillin, and making penicillin concn is 2U/ml;
(4) inserting the zymomonas mobilis seed liquor, is to be cultured to fortune under the condition of 200rpm at 30 ± 5 ℃, mixing speed
The concentration of moving fermentation single cell bacterium reaches 1.0 * 10
8More than individual cell/ml;
(5) stream adds the syrup that described step (2) obtains, the mass concentration of the speed control that syrup stream adds total reducing sugar in fermented liquid is below 10%, treat in the fermented liquid that the alcoholic acid volumetric concentration is to stop stream at 7% o'clock to add, adopt batch fermentation, treat that the alcoholic acid volumetric concentration is 12.6% in the fermented liquid, the mass concentration of residual total reducing sugar is below 2.0%, and the mass concentration of residual reducing sugar is 0.2% to stop fermentation when following;
(6) rectifying obtains ethanol, again through dehydration, gets alcohol fuel.The ethanol yield is 93.1%.
Zymomonas mobilis culture of seed liquid used among each embodiment is:
The former bacterial classification of zymomonas mobilis
↓
Slant tube
↓
Liquid tube is cultivated
↓
Triangular flask is cultivated
↓
The Ka Shi jar is cultivated
The culture of seed liquid method in industry is a routine operation, and used substratum is RM liquid nutrient medium (2% glucose, 1% yeast extract, 0.2% potassium primary phosphate) and RM solid medium (2% glucose, 1% yeast extract, 0.2% phosphoric acid dioxy potassium, 1.8% agar).
Claims (3)
1. with the method for zymomonas mobilis fermenting sweet sorghum stem stalk concentrated juice production alcohol fuel, it is characterized in that forming by following step:
(1) mechanical expression sweet sorghum stalk obtains liquid glucose;
(2) concentrate described liquid glucose and obtain syrup, make that the mass concentration of total reducing sugar reaches 45%-65% in the syrup;
(3) concentration of the syrup of regulating step (2) acquisition, the mass concentration that obtains total reducing sugar is rare syrup of 5%-15%, regulates pH to 3.5-5.0, and adds penicillin, making penicillin concn is 0.1-1000U/ml;
(4) insert the zymomonas mobilis seed liquor, cultivate;
(5) stream adds the syrup that described step (2) obtains, and ferments;
(6) rectifying obtains ethanol, again through dehydration, gets alcohol fuel.
2. method of producing alcohol fuel according to claim 1 with zymomonas mobilis fermenting sweet sorghum stem stalk concentrated juice, it is characterized in that described step (4) is: inserting the zymomonas mobilis seed liquor, is that the concentration that is cultured to zymomonas mobilis under the condition of 40-500rpm reaches 1.0 * 10 at 30 ± 5 ℃, mixing speed
8More than individual cell/ml.
3. method of producing alcohol fuel according to claim 1 with zymomonas mobilis fermenting sweet sorghum stem stalk concentrated juice, it is characterized in that described step (5) is: stream adds the syrup that described step (2) obtains, the mass concentration of the speed control that syrup stream adds total reducing sugar in fermented liquid is below 12%, treat in the fermented liquid that the alcoholic acid volumetric concentration is to stop stream at 7%～8% o'clock to add, adopt batch fermentation, semicontinuous fermentation, continuously ferment or have the fermentation mode fermentation of zymomonas mobilis reuse, treat that the alcoholic acid volumetric concentration is 12.0%-14.0% in the fermented liquid, the mass concentration of residual total reducing sugar is below 2.0%, and the mass concentration of residual reducing sugar is 0.2% to stop fermentation when following.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102417251A CN101914575A (en) | 2010-07-30 | 2010-07-30 | Method for producing ethanol fuel by fermenting sweet sorghum stalk concentrated juice by using zymomonas mobilis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102417251A CN101914575A (en) | 2010-07-30 | 2010-07-30 | Method for producing ethanol fuel by fermenting sweet sorghum stalk concentrated juice by using zymomonas mobilis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101914575A true CN101914575A (en) | 2010-12-15 |
Family
ID=43322213
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102417251A Pending CN101914575A (en) | 2010-07-30 | 2010-07-30 | Method for producing ethanol fuel by fermenting sweet sorghum stalk concentrated juice by using zymomonas mobilis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101914575A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0047641A2 (en) * | 1980-09-05 | 1982-03-17 | George Weston Limited | Ethanol production by high performance bacterial fermentation |
| CN101497900A (en) * | 2008-01-30 | 2009-08-05 | 中粮集团有限公司 | Method for preparing ethanol |
-
2010
- 2010-07-30 CN CN2010102417251A patent/CN101914575A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0047641A2 (en) * | 1980-09-05 | 1982-03-17 | George Weston Limited | Ethanol production by high performance bacterial fermentation |
| CN101497900A (en) * | 2008-01-30 | 2009-08-05 | 中粮集团有限公司 | Method for preparing ethanol |
Non-Patent Citations (2)
| Title |
|---|
| 《The 18th Annual Meeting of the Thai Society for Biotechnology "Biotechnology: Benefits & Bioethics"》 20061103 Chaichalerm jongsomchai et al. Ethanol Production form Sweet Sorghum Stem Juice by Zymomonas mobilis TISTR 548 and Saccharomyces cerevisiae NP 01 in Batch Fermentation 第VI-P-22篇第1-2页 1-3 , * |
| 《山东轻工业学院学报》 20070630 关志炜等 甜高粱茎秆汁液酒精发酵条件的初步研究 81-83 1-3 第21卷, 第2期 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11753658B2 (en) | Pichia stipitis strain and cultures and uses of the same | |
| CN102174602B (en) | Method for producing L-lactic acid through biomass fermentation | |
| CN101824395B (en) | Method for culturing fermentation seed liquid by adopting solid straws as carbon source | |
| CN102399826A (en) | Comprehensive utilizing method of sweet sorghum stalks | |
| CN102076860A (en) | Method of producing yeast biomass | |
| JP5711873B2 (en) | Simultaneous saccharification and fermentation of cellulosic materials | |
| CN101638673A (en) | Method for manufacturing alcohol by utilizing fermentation of plant straws | |
| CN104805136B (en) | A method of citric acid is produced using lignocellulosic material | |
| Imam et al. | Fermentation kinetics and ethanol production from different sweet sorghum varieties | |
| US8679803B2 (en) | Glucose conversion to ethanol via yeast cultures and bicarbonate ions | |
| CN103898166A (en) | Method of producing ethanol | |
| CN101709309B (en) | Method for combined fermentation of ethanol and xylitol | |
| CN101696429B (en) | Method for producing butanol by hydrolyzing and fermenting waste crops rich in hemicellulose | |
| CN109628503A (en) | A method of use corn and corn stover as the comprehensive production ethyl alcohol of raw material | |
| CN101886092B (en) | Method for fermenting cellulosic ethanol by taking DDGS as nutrient | |
| CN101914575A (en) | Method for producing ethanol fuel by fermenting sweet sorghum stalk concentrated juice by using zymomonas mobilis | |
| CN105331641A (en) | Method for preparing succinic acid by using water hyacinth as fermentation raw material | |
| CN112662710B (en) | Method for producing L-lactic acid by continuous fermentation of lignocellulose | |
| CN116555359B (en) | Method for producing fuel ethanol by bioconversion of poplar biomass | |
| CN102146419A (en) | Method for producing ethanol by using straw as raw material | |
| CN101955889A (en) | Cadmium salt resistant Candida tropicalis and application thereof | |
| CN102453732B (en) | Method for preparing fuel ethanol from fermentable sugars in fresh straw juice | |
| CN102154378A (en) | Fermentation production method of xylitol | |
| Vidyavathi et al. | Optimization of fermentation parameters of acid hydrolysate of pods of Samanea saman for ethanol production | |
| Singh Kocher et al. | Development of a fermentation process for utilization of Emblica Officinalis Gaertn (Amla) candy waste for production of natural vinegar |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20101215 |