CN101912027B - Method for preparing protein hydrolyzate from abalone viscera through complex enzyme hydrolysis - Google Patents

Method for preparing protein hydrolyzate from abalone viscera through complex enzyme hydrolysis Download PDF

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Publication number
CN101912027B
CN101912027B CN 201010269348 CN201010269348A CN101912027B CN 101912027 B CN101912027 B CN 101912027B CN 201010269348 CN201010269348 CN 201010269348 CN 201010269348 A CN201010269348 A CN 201010269348A CN 101912027 B CN101912027 B CN 101912027B
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complex enzyme
enzyme
abalone
protein hydrolyzate
hydrolysis
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CN101912027A (en
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江铭福
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FUZHOU RIXING AQUATIC FOOD Co Ltd
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FUZHOU RIXING AQUATIC FOOD Co Ltd
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Abstract

The invention discloses a method for preparing protein hydrolyzate from abalone viscera through complex enzyme hydrolysis, bitterness shielding and other technologies. Complex enzymes comprise alkali protease and papain. The protein hydrolyzate is prepared from abalone viscera by cleaning, pulping, hydrolyzing with the enzymes, inactivating the enzymes and shielding bitterness by using ethyl maltol in sequence. The method effectively utilizes a side product, namely viscera, generated during abalone processing, and the bitterness of the protein hydrolyzate is avoided; the prepared protein hydrolyzate has high content of free amino acid, high absorptivity of human body, rich nutrition and delicious flavor, and can be used as an additive to be widely applied to products such as cans, sauces,fodders, plant nutrient solutions and the like.

Description

A kind of method of preparing protein hydrolyzate from abalone viscera through complex enzyme hydrolysis
Technical field
The present invention is specifically related to a kind of method of preparing protein hydrolyzate from abalone viscera through complex enzyme hydrolysis.
Background technology
The abalone delicious flavour, nutritious, per 100 gram Fresh abalone fishes contain protein 12.6 grams, fat 0.8 gram, carbohydrate 6.6 grams, and contain the mineral matter and the multivitamins such as calcium, phosphorus, iron, zinc of needed by human body." dietetic materia medica " record, abalone goes into the logical stasis of blood of liver, goes into intestines and washs dirt, does not hinder vigour, establishing-Yang, living hundred arteries and veins.Abalone has higher nutritive value and medical value, is that amino acid content is the most comprehensive in the ocean, one of fatty, that cholesterol level is minimum biology.
The abalone internal organ usually are used as leftover bits and pieces and discard, and wherein discarded leftover bits and pieces accounts for more than 19% of full abalone quality in the tin product process.Contain a large amount of protein in the abalone internal organ, contain trace element and vitamin etc. in addition.Its protein can be hydrolyzed into and be the peptide and the amino acid that can directly absorbed and utilize by animal, and discharges the food calling material of numerous fish and shrimp.Therefore, the abalone internal organ are good protein resources.
At present the utilization to leftover bits and pieces such as abalone internal organ mainly is to produce the feed fish meal, and valuable ingredients is wherein still underused.Separation and purification, polysaccharide that the research of abalone internal organ mainly contains alkaline phosphatase extract and anti-oxidant research, acid hydrolysis and mailland reaction product analysis thereof etc.; Acid hydrolysis is mainly adopted in hydrolysis for internal organ; But the amino acid whose yield of acid hydrolysis is lower, and the local flavor of hydrolyzate is not good enough.
Publication number be CN 101433345A disclosure of the Invention a kind of method of comprehensive utilization of grass carp harslet; The main process of this method is: grass carp harslet is after cleaning out; Take off the raw meat deodorization with acetic acid, sodium chloride and sodium sulfite mixed liquor and handle, smash trypsin digestion to pieces; Centrifugal, isolate the upper strata crude fish oil.Crude fish oil can make refined fish oil through refining.Lower floor's enzymolysis liquid behind vacuum filtration, with active carbon once more debitterize take off raw meat, spray-drying makes fish protein polypeptide powder and amino acid powder then.Polypeptide powder mainly contains dipeptides and tripeptides, and is rich in multiple mineral element, be prone to digested and assimilated by enteron aisle, and be a kind of high-quality nutriment.Amino acid powder contains the amino acid A wide selection of colours and designs, can be the additive of various amino acid nutrient enhanced products.Process conditions of the present invention are gentle, cause the protein loss bigger but take off raw meat with the active carbon debitterize.
Publication number be CN 1334258A disclosure of the Invention a kind of production method of plant nutrient agent, it is characterized in that with marine organisms and pluck be raw material, after crushed, add hydrolysising protease and carry out enzyme digestion reaction, obtain finished product through filtering, concentrating again.Adopt single hydrolysising protease, the proteolysis yield is low.
Summary of the invention
Primary and foremost purpose of the present invention is to provide a kind of abalone internal organ complex enzyme hydrolysis, bitterness shielding and other technologies to prepare the method for protein hydrolyzate to the problem that above-mentioned prior art exists; This method has effectively been utilized the accessory substance-internal organ in the abalone process; Avoided the bitter taste of protein hydrolyzate, the protein hydrolyzate free aminoacid content that makes is high, the human absorptivity is high, nutritious, local flavor is delicious.
For realizing above-mentioned purpose; The present invention provides a kind of complex enzyme of being made up of alkali protease and papain; The method of hydrolysis preparing protein hydrolyzate from abalone viscera through, this preparation method's flow process is: to the abalone internal organ clean successively, making beating, complex enzyme for hydrolyzing, the enzyme that goes out, shielding taste handle.Wherein the concrete steps of complex enzyme hydrolysis hydrolysis are: the abalone internal organ to after cleaning, pulling an oar add moisture; The ratio of regulating solid-to-liquid ratio is 0.5 ~ 1.5:1 ~ 1.5; Be preferably 0.5 ~ 1:1, add the pH conditioning agent and regulate pH to 7 ~ 8, add complex enzyme then; Be heated to temperature when being 45 ~ 50 ℃, enzymolysis time 4 ~ 5h carries out enzymolysis processing.Wherein said pH conditioning agent is selected one or more in citric acid, natrium citricum, potassium citrate, NaOH, potash, sodium carbonate, the sodium sesquicarbonate.Described enzyme inactivating method is that the slurry behind the enzymolysis is placed in the jacketed pan, is heated to 85 ℃, and enzyme 5 ~ 10min goes out.It is to adopt ethyl maltol shielding bitter taste that said shielding taste is handled, and addition is 0.005% ~ 0.01%.The complex enzyme that is adopted is made up of alkali protease and papain, and its composition by weight proportioning is 1.5 ~ 2.5:1 ~ 2, is preferably 2 ~ 2.5:1 ~ 1.5, further is preferably 2:1, and addition is 0.5% ~ 0.6%, is preferably 0.55%, further is preferably 0.5%.The protein hydrolyzate that makes through above method can be used as a kind of additive and is applied in widely in the goods such as can, sauce, feed, plant nutrition liquid.
The invention has the advantages that:
1, the hydrolysis of complex enzyme of the present invention is complete, and protein hydrolysis ratio is high, and the amino nitrogen yield reaches 65.4 ~ 71.6%.
2, the method for shielding bitter taste of the present invention has not only effectively been removed the bitter taste of hydrolyzate, increases bright effect in addition.
3, complex enzyme low in raw material cost of the present invention is easy to get, and preparation method's technology of protein hydrolyzate is simple, easy to operate, easy control of process conditions, and the protein hydrolyzate of preparation is quality controllable, has good practical value and application prospect.
Description of drawings
Fig. 1 is the yield of the contained amino nitrogen of protein hydrolyzate of preparation.
The specific embodiment
Embodiment 1
1, with after the abalone internal organ taking-up cleaning, use the beater coarse crushing earlier, put into colloid mill then and carry out miniaturization processing 10min, the rotating speed of colloid mill is 3000 rev/mins, makes abalone internal organ slurry;
2, get a certain amount of abalone internal organ slurry, add water and stir, the adjusting solid-to-liquid ratio is 1:1;
3, adding mass concentration is the sodium hydroxide solution of 1mol/L, regulates the pH to 8 of mixed liquor;
4, add the complex enzyme that is mixed by alkali protease and papain, the enzyme hydrolysis processing is carried out in heating, stirs once in every interval 10-15 minute; Obtain enzyme hydrolyzate; Wherein, alkali protease and papain weight portion proportioning are 2:1, and the addition of complex enzyme is 0.5%; The enzyme hydrolysis treatment temperature is 45 ℃, and the time is 5h;
5, hydrolyzate is placed in the jacketed pan, be heated to 85 ℃, the enzyme 10min that goes out makes the complex enzyme deactivation, obtains protein hydrolyzate;
6, the ethyl maltol of adding 0.006% in the protein hydrolyzate of handling through the enzyme that goes out stirs;
7, the protein hydrolyzate that makes is added in the abalone can by a certain percentage, both increased the local flavor of abalone, improved the nutritive value of product again.
Embodiment 2
1, with after the abalone internal organ taking-up cleaning, use the beater coarse crushing earlier, put into colloid mill then and carry out miniaturization processing 10min, the rotating speed of colloid mill is 3000 rev/mins, makes abalone internal organ slurry;
2, get a certain amount of abalone internal organ slurry, add water and stir, the adjusting solid-to-liquid ratio is 3:2;
3, adding mass concentration is the sodium hydroxide solution of 1mol/L, regulates the pH to 7.5 of mixed liquor;
4, add the complex enzyme that is mixed by alkali protease and papain, the enzyme hydrolysis processing is carried out in heating, stirs once in every interval 10-15 minute; Obtain enzyme hydrolyzate; Wherein, alkali protease and papain weight portion proportioning are 1:1, and the addition of complex enzyme is 0.6%; The enzyme hydrolysis treatment temperature is 50 ℃, and the time is 4.5h;
5, hydrolyzate is placed in the jacketed pan, be heated to 85 ℃, the enzyme 10min that goes out makes the complex enzyme deactivation, obtains protein hydrolyzate;
6, the ethyl maltol of adding 0.006% in the protein hydrolyzate of handling through the enzyme that goes out stirs;
7, get a certain amount of protein hydrolyzate, ratio on request adds flavorings such as salt, sugar, sweet fermented flour sauce, peanut butter, oyster sauce, and stir back packing, sterilization make abalone sauce.
Embodiment 3
1, with after the abalone internal organ taking-up cleaning, use the beater coarse crushing earlier, put into colloid mill then and carry out miniaturization processing 10min, the rotating speed of colloid mill is 3000 rev/mins, makes abalone internal organ slurry;
2, get a certain amount of abalone internal organ slurry, add water and stir, the adjusting solid-to-liquid ratio is 3:1;
3, adding mass concentration is the sodium hydroxide solution of 1mol/L, regulates the pH to 7.5 of mixed liquor;
4, add the complex enzyme that is mixed by alkali protease and papain, the enzyme hydrolysis processing is carried out in heating, stirs once in every interval 10-15 minute; Obtain enzyme hydrolyzate; Wherein, alkali protease and papain weight portion proportioning are 2:1, and the addition of complex enzyme is 0.7%; The enzyme hydrolysis treatment temperature is 50 ℃, and the time is 5h;
5, hydrolyzate is placed in the jacketed pan, be heated to 85 ℃, the enzyme 10min that goes out makes the complex enzyme deactivation, obtains protein hydrolyzate;
6, the ethyl maltol of adding 0.006% in the protein hydrolyzate of handling through the enzyme that goes out stirs;
7, protein hydrolyzate and the residue after the hydrolysis with preparation concentrates together, dries, sterilizes, and adds by a certain percentage in the animal feed, improves the protein content of animal feed.
Embodiment 4
1, with after the abalone internal organ taking-up cleaning, use the beater coarse crushing earlier, put into colloid mill then and carry out miniaturization processing 10min, the rotating speed of colloid mill is 3000 rev/mins, makes abalone internal organ slurry;
2, get a certain amount of abalone internal organ slurry, add water and stir, the adjusting solid-to-liquid ratio is 1:1;
3, adding mass concentration is the sodium hydroxide solution of 1mol/L, regulates the pH to 7 of mixed liquor;
4, add the complex enzyme that is mixed by alkali protease and papain, the enzyme hydrolysis processing is carried out in heating, stirs once in every interval 10-15 minute; Obtain enzyme hydrolyzate; Wherein, alkali protease and papain weight portion proportioning are 1:1, and the addition of complex enzyme is 0.6%; The enzyme hydrolysis treatment temperature is 45 ℃, and the time is 5h;
5, hydrolyzate is placed in the jacketed pan, be heated to 85 ℃, the enzyme 10min that goes out makes the complex enzyme deactivation, obtains protein hydrolyzate;
6, the ethyl maltol of adding 0.005% in the protein hydrolyzate of handling through the enzyme that goes out stirs;
7, protein hydrolyzate is concentrated by a certain percentage after, process satisfactory plant nutrition liquid.
Performance test:
The performance indications of the protein hydrolyzate of preparation detect according to following method, and testing result is seen table 1.
The amino-acid nitrogen yield is calculated by following formula:
Amino-acid nitrogen yield=N 1/ N 2* 100%
In the formula: N 1Amino acid nitrogen content in the-hydrolyzate; Crude protein nitrogen content in the raw material
The mensuration of amino-acid nitrogen in the hydrolyzate: formaldehyde-potentiometric titration
Crude protein nitrogen analysis in the raw material: Kjeldahl
Yield result by amino nitrogen shows:
The hydrolysis of complex enzyme of the present invention is complete, and protein hydrolysis ratio is high, and the amino nitrogen yield reaches 65.4 ~ 71.6%.
The above is merely preferred embodiment of the present invention, and all equalizations of doing according to claim of the present invention change and modify, and all should belong to covering scope of the present invention.

Claims (1)

1. the method for a preparing protein hydrolyzate from abalone viscera through complex enzyme hydrolysis, it is characterized in that: this preparation method's flow process is: to the abalone internal organ clean successively, making beating, complex enzyme for hydrolyzing, the enzyme that goes out, shielding taste handle;
The concrete steps of described complex enzyme hydrolysis hydrolysis are: the abalone internal organ after cleaning, pulling an oar add moisture, regulate solid-to-liquid ratio, add the pH conditioning agent and regulate pH to 7 ~ 8, add complex enzyme then, heat and carry out enzymolysis processing; Described solid-to-liquid ratio is: 0.5 ~ 1.5:1 ~ 1.5; Described pH conditioning agent is selected one or more in citric acid, natrium citricum, potassium citrate, NaOH, potash, sodium carbonate, the sodium sesquicarbonate; The condition of described complex enzyme for hydrolyzing is 45 ~ 50 ℃ of hydrolysis temperatures, enzymolysis time 4 ~ 5h; Described complex enzyme is made up of alkali protease and papain, and its composition by weight proportioning is 1.5 ~ 2.5:1 ~ 2; The total addition level of said alkali protease and papain is 0.5% ~ 0.6%;
Described enzyme inactivating method is that the slurry behind the enzymolysis is placed in the jacketed pan, is heated to 85 ℃, and enzyme 5 ~ 10min goes out;
It is to adopt ethyl maltol shielding bitter taste that said shielding taste is handled, and addition is 0.005% ~ 0.01%.
CN 201010269348 2010-09-01 2010-09-01 Method for preparing protein hydrolyzate from abalone viscera through complex enzyme hydrolysis Expired - Fee Related CN101912027B (en)

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CN102178027B (en) * 2011-05-13 2012-09-05 陈锦权 Method for separating and purifying abalone proteins
WO2016153362A1 (en) * 2015-03-24 2016-09-29 The New Zealand Institute For Plant And Food Research Limited Water-soluble paua extract
CN104970342B (en) * 2015-07-14 2017-04-26 福建省水产研究所 Abalone viscus sauce and processing method thereof
CN105815695A (en) * 2015-12-15 2016-08-03 浙江海洋学院 Formula and processing technology for can of braised skipjack in soy sauce
CN107498067B (en) * 2017-09-21 2019-10-18 厦门医学院 The method that Bao internal organ degradation product green quickly prepares nano silver colloidal sol
CN110897037A (en) * 2019-11-27 2020-03-24 武汉新华扬生物股份有限公司 Preparation method of fish viscera enzymolysis product
CN110934241A (en) * 2019-12-18 2020-03-31 威海长青海洋科技股份有限公司 Feed additive for solving growth deficiency of juvenile abalone shells and using method
CN112790368A (en) * 2021-01-13 2021-05-14 百珍堂生物科技(浙江)有限公司 Compound abalone sauce and preparation method thereof
CN113331367A (en) * 2021-06-30 2021-09-03 百珍堂生物科技(浙江)有限公司 Abalone viscera treatment method, fermentation treatment method and preparation method thereof
CN114230381A (en) * 2021-12-29 2022-03-25 韩德新 Asterina pectinifera/echinocandin hydrolysate, water-soluble fertilizer and preparation method thereof
CN114634962B (en) * 2022-04-29 2024-03-19 韩耀辉 Preparation method of abalone viscera protein peptide

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CN1268320A (en) * 2000-01-07 2000-10-04 湛江海洋大学 Scaled sardine protein beverage and its production method
CN1288675A (en) * 2000-06-14 2001-03-28 湛江海洋大学 Multienzyme fish hydrolyzing method and fish beverage producing process
CN101326990A (en) * 2008-07-17 2008-12-24 荣成市杰通水产食品有限公司 Seafood sauce and process for producing the same
CN101455264A (en) * 2008-11-15 2009-06-17 山东好当家海洋发展股份有限公司 Preparation method of sea-ear active peptide

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1268320A (en) * 2000-01-07 2000-10-04 湛江海洋大学 Scaled sardine protein beverage and its production method
CN1288675A (en) * 2000-06-14 2001-03-28 湛江海洋大学 Multienzyme fish hydrolyzing method and fish beverage producing process
CN101326990A (en) * 2008-07-17 2008-12-24 荣成市杰通水产食品有限公司 Seafood sauce and process for producing the same
CN101455264A (en) * 2008-11-15 2009-06-17 山东好当家海洋发展股份有限公司 Preparation method of sea-ear active peptide

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