Summary of the invention
Technical problem to be solved by this invention provide a kind of compatibility rationally, have no side effect, have the pharmaceutical composition of expelling wind and removing dampness, relaxing muscles and tendons and activating QI and blood in the collateral, antalgic effect.
Another technical problem to be solved by this invention has provided multiple aforesaid preparation of drug combination method with expelling wind and removing dampness, relaxing muscles and tendons and activating QI and blood in the collateral, antalgic effect.
Another technical problem to be solved by this invention has provided aforesaid the have pharmaceutical composition of expelling wind and removing dampness, relaxing muscles and tendons and activating QI and blood in the collateral, antalgic effect or the pharmaceutical preparation that aforesaid preparation method is made.
Pharmaceutical composition of the present invention can be used for the treatment of rheumatic arthritis, rheumatoid arthritis, numb limbs and tense tendons, back cold type of pain, scapulohumeral periarthritis, neck-shoulder pain, lumbago and leg pain, sciatica, trigeminal neuralgia, hyperosteogeny, prolapse of lumbar intervertebral disc, postpartum apoplexy etc.
Technical problem to be solved by this invention is to realize by following technical scheme.The present invention is the pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, is characterized in, it is to be made by following Chinese medicine raw materials by weight proportion,
Rhizoma Alpiniae Officinarum 0.1~15; Flos Caryophylli 0.1~15; Radix Angelicae Sinensis 0.1~15;
Radix Schefflerae Arboricolae 0~30; Pterospermi Heterophylli 0~30.
Above-described pharmaceutical composition, the preferred weight proportioning of each raw material of Chinese medicine is:
Rhizoma Alpiniae Officinarum 0.5~6; Flos Caryophylli 0.5~3; Radix Angelicae Sinensis 0.5~9;
Radix Schefflerae Arboricolae 0~20; Pterospermi Heterophylli 0~20.
In the above-described drug regimen composition formula of the present invention, Rhizoma Alpiniae Officinarum, Flos Caryophylli and Radix Angelicae Sinensis 3 flavor raw materials are formed square substantially; Radix Schefflerae Arboricolae, Pterospermi Heterophylli 2 flavor raw materials are optional raw materials; 2 flavors any in the optional raw material or 2 kinds can carry out prescription with the basic side of above-mentioned 3 flavors; When the weight proportion in the optional raw material is selected " 0 ", represent and do not contain this raw material in the prescription.
Technical problem to be solved by this invention can also realize by following technical scheme.Above-described pharmaceutical composition is characterized in, it is to be made by following Chinese medicine raw materials by weight proportion,
Rhizoma Alpiniae Officinarum 0.1~15; Flos Caryophylli 0.1~15; Radix Angelicae Sinensis 0.1~15;
Radix Schefflerae Arboricolae 0.1~30 and Pterospermi Heterophylli 0.1~30.
The pharmaceutical composition of above-described expelling wind and removing dampness, activating collaterals to relieve pain, the preferred weight proportioning of each raw material of Chinese medicine is:
Rhizoma Alpiniae Officinarum 0.5~6; Flos Caryophylli 0.5~3; Radix Angelicae Sinensis 0.5~9
Radix Schefflerae Arboricolae 0.5~20; With/or Pterospermi Heterophylli 0.5~20.
In the pharmaceutical composition of above-described expelling wind and removing dampness, activating collaterals to relieve pain, described Radix Schefflerae Arboricolae has another name called Radix Schefflerae Arboricolae (Caulis et Folium Schefflerae Arboricolae), derive from the Araliaceae schefflera and belong to (Schefflera) various plants, mainly contain Radix Schfflerae kwangisensis S.kwangsiensis Merr.Ex Li, spend schefflera S.leacantha Vig in vain, Schefflera venulosa (Wight et Arn.) Harms S.venulosa Wight et Arn., schefflera S.octophylla Harms, ball preface schefflera S.glomerulata L. and schefflera arboricola S.arboricola Hayata etc.
At above-described expelling wind and removing dampness, in the pharmaceutical composition of activating collaterals to relieve pain, described Pterospermi Heterophylli is Sterculiaceae Folium potentillae velutinae (Radix Potentillae Fulgentis) tree (Pterospermum heterophyllum Hance), or Hamamelidaceae Pterospermi Heterophylli (Semiliquidambar cathayensis Chang), or Moraceae Bai Guimu (Artocarpus hypargyraea Hance) and two color Artocarpus heterophyllus Lam (Red Hill prunus mume (sieb.) sieb.et zucc.s, Artocarpus styracifolius Pierre), or Lauraceae sassafrases (Sassafras tzumu Hemsl.), or Araliaceae tree ginseng (Dendropanax chevalieri (Vig) Merr.) and change Ye Shucan (Dendropanax proteus Benth.).
Pharmaceutical composition of the present invention can be prepared according to conventional method of the prior art, extracts the active ingredient of each raw material of Chinese medicine, adds needed pharmaceutic adjuvant, makes the medicine of clinical any oral or exterior-applied formulation applicatory.Pharmaceutical composition of the present invention preferably is prepared by the method for the following stated.Drug combination preparation of the present invention or can be the medicament of acceptable clinically any oral or exterior-applied formulation by the preparation that the inventive method makes, its dosage form includes but not limited to capsule, soft capsule, tablet, pill, drop pill, granule, powder, patch, unguentum, gel, liquid preparation.
The pharmaceutical composition of expelling wind and removing dampness of the present invention, activating collaterals to relieve pain can be prepared by following method, and its step is as follows:
(1) getting whole raw material of Chinese medicine by weight ratio carries out decocting and gets the water extract; The extract of perhaps fetching water concentrates the back precipitate with ethanol, gets the precipitate with ethanol supernatant;
(2) make oral or external preparation after water extract or the precipitate with ethanol supernatant concentration; Perhaps, drying is made oral or external preparation after water intaking extract or the precipitate with ethanol supernatant concentration.
Pharmaceutical composition with expelling wind and removing dampness, relaxing muscles and tendons and activating QI and blood in the collateral, antalgic effect of the present invention can also be prepared by following method, and its step is as follows:
(1) get whole raw material of Chinese medicine by weight ratio and carry out alcohol extraction, alcohol extract concentrate the alcohol extraction concentrated solution; Perhaps, alcohol extraction concentrated solution water precipitating, the water precipitating supernatant concentration gets the alcohol extracting-water precipitating concentrated solution;
(2) make oral alcohol extraction concentrated solution or alcohol extracting-water precipitating concentrated solution or external preparation; Perhaps, with alcohol extraction concentrated solution or alcohol extracting-water precipitating concentrated solution drying, make oral or external preparation.
The pharmaceutical composition of expelling wind and removing dampness of the present invention, activating collaterals to relieve pain can also be prepared by following method, and its step is as follows:
(1) gets flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, the Radix Angelicae Sinensis or 2 flavors or 3 flavors by weight ratio and extract volatile oil;
(2) medicinal residues merge alcohol extraction with the residue raw material of Chinese medicine, and alcohol extract concentrates, and adds volatile oil, makes oral or external preparation; Perhaps with the alcohol extract concentrate drying, add behind the enclose or the volatile oil of enclose not, make oral or external preparation.
The pharmaceutical composition of expelling wind and removing dampness of the present invention, activating collaterals to relieve pain can also be prepared by following method, gets whole raw material of Chinese medicine by weight ratio and carries out alcohol extraction, and alcohol extract concentrates; The medicinal residues decocting, the water extract concentrates; Above-mentioned alcohol extraction concentrated solution, water extracting liquid are merged, make oral or external preparation; Perhaps, with alcohol extraction concentrated solution, water extracting liquid drying, make oral or external preparation.
The pharmaceutical composition of expelling wind and removing dampness of the present invention, activating collaterals to relieve pain can also be prepared by following method, and its step is as follows:
(1) gets flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, the Radix Angelicae Sinensis or 2 flavors or 3 flavors by weight ratio and extract volatile oil;
(2) medicinal residues merge decocting with the residue raw material of Chinese medicine, the water extract concentrate water extracting liquid; Perhaps, the water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration gets the water extract-alcohol precipitation concentrated solution;
(3) get water extracting liquid or water extract-alcohol precipitation concentrated solution, add volatile oil, make oral or external preparation; Perhaps,, add behind the enclose or the volatile oil of enclose not, make oral or external preparation water extracting liquid or water extract-alcohol precipitation concentrated solution drying.
The pharmaceutical composition of expelling wind and removing dampness of the present invention, activating collaterals to relieve pain can also be prepared by following method, and its step is as follows:
(1) gets flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, the Radix Angelicae Sinensis or 2 flavors or 3 flavors by weight ratio and extract volatile oil;
(2) medicinal residues merge alcohol extraction with the residue raw material of Chinese medicine, and alcohol extract concentrates;
(3) medicinal residues decocting, water extract concentrate water extracting liquid; Perhaps, the water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration gets the water extract-alcohol precipitation concentrated solution;
(4) water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution and volatile oil are merged, make oral or external preparation; Perhaps, with water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution drying,
Add behind the enclose or the volatile oil of enclose not, make oral or external preparation.
The pharmaceutical composition of expelling wind and removing dampness of the present invention, activating collaterals to relieve pain can also be prepared by following method, and its step is as follows:
(1) get 1 in Radix Schefflerae Arboricolae, Pterospermi Heterophylli flavor by weight ratio or 2 flavor water are carried, the water extract concentrate water extracting liquid; Perhaps water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration, the water extract-alcohol precipitation concentrated solution;
(2) get flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, the Radix Angelicae Sinensis or 2 flavors or 3 flavors by weight ratio and extract volatile oil, the medicinal residues alcohol extraction, alcohol extract concentrates;
(3) water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution and volatile oil are merged, make oral or external preparation; Perhaps,, add behind the enclose or the volatile oil of enclose not, make oral or external preparation water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution drying.
The pharmaceutical composition of expelling wind and removing dampness of the present invention, activating collaterals to relieve pain can also be prepared by following method, and its step is as follows:
(1) get 1 in Radix Schefflerae Arboricolae, Pterospermi Heterophylli flavor or 2 flavor alcohol extractions by weight ratio, alcohol extract concentrate the alcohol extraction concentrated solution; Perhaps, alcohol extraction concentrated solution water precipitating, the water precipitating supernatant concentration gets the alcohol extracting-water precipitating concentrated solution;
(2) get 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis flavor or 2 flavors or 3 flavors by weight ratio and extract volatile oil, medicinal residues water is carried, the water extract concentrate water extracting liquid; Perhaps water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration, the water extract-alcohol precipitation concentrated solution;
(3) with above-mentioned alcohol extraction concentrated solution or alcohol extracting-water precipitating concentrated solution, water extracting liquid or water extract-alcohol precipitation concentrated solution and volatile oil merging, make oral or external preparation; Perhaps,, add behind the enclose or the volatile oil of enclose not, make oral or external preparation alcohol extraction concentrated solution or alcohol extracting-water precipitating concentrated solution, water extracting liquid or water extract-alcohol precipitation concentrated solution drying.
The pharmaceutical composition of expelling wind and removing dampness of the present invention, activating collaterals to relieve pain can also be prepared by following method, and its step is as follows:
(1) get 1 in Radix Schefflerae Arboricolae, Pterospermi Heterophylli flavor by weight ratio or 2 flavor water are carried, the water extract concentrate water extracting liquid; Perhaps water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration, the water extract-alcohol precipitation concentrated solution;
(2) residue raw material of Chinese medicine alcohol extraction, alcohol extract concentrates;
(3) water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution are merged, make oral or external preparation; Perhaps, with water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution drying, make oral or external preparation.
The pharmaceutical composition of expelling wind and removing dampness of the present invention, activating collaterals to relieve pain can also be prepared by following method, gets whole raw material of Chinese medicine by weight ratio and pulverizes, and makes oral or external preparation.
The pharmaceutical composition of expelling wind and removing dampness of the present invention, activating collaterals to relieve pain can also be prepared by following method, and its step is as follows:
(1) gets flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, the Radix Angelicae Sinensis or 2 flavors or all or part of powder of beating of 3 flavors by weight ratio;
(2) residue raw material of Chinese medicine alcohol extraction together, alcohol extract concentrates back and above-mentioned powder mixing, makes oral or external preparation.
Expelling wind and removing dampness of the present invention, activating collaterals to relieve pain pharmaceutical composition can also be prepared by following method, its step is as follows:
(1) gets flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, the Radix Angelicae Sinensis or 2 flavors or all or part of powder of beating of 3 flavors by weight ratio;
(2) the residue raw material of Chinese medicine together water carry, the water extract concentrate water extracting liquid; Perhaps water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration, the water extract-alcohol precipitation concentrated solution;
(3) with water extracting liquid or water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder mixing, make oral or external preparation.Perhaps, with water extracting liquid or water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder mixing, drying is made oral or external preparation.
Expelling wind and removing dampness of the present invention, activating collaterals to relieve pain pharmaceutical composition can also be prepared by following method, its step is as follows:
(1) gets flavor of 1 in Radix Schefflerae Arboricolae, the Pterospermi Heterophylli or all or part of powder of beating of 2 flavors by weight ratio;
(2) get flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, the Radix Angelicae Sinensis or 2 flavors or 3 flavors by weight ratio and extract volatile oil; Medicinal residues merge alcohol extraction with the residue raw material of Chinese medicine, and alcohol extract concentrates;
(3) with alcohol extraction concentrated solution, above-mentioned medicated powder and volatile oil mixing, make oral or external preparation; Perhaps, with alcohol extraction concentrated solution, above-mentioned medicated powder mixing, drying adds behind the enclose or the volatile oil of enclose not, makes oral or external preparation.
The pharmaceutical composition of expelling wind and removing dampness of the present invention, activating collaterals to relieve pain can also be prepared by following method, and its step is as follows:
(1) gets flavor of 1 in Radix Schefflerae Arboricolae, the Pterospermi Heterophylli or all or part of powder of beating of 2 flavors by weight ratio;
(2) get flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, the Radix Angelicae Sinensis or 2 flavors or 3 flavors by weight ratio and extract volatile oil; Medicinal residues merge water with the residue raw material of Chinese medicine to be carried, the water extract concentrate water extracting liquid; Perhaps water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration, the water extract-alcohol precipitation concentrated solution;
(3) with water extracting liquid or water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder and volatile oil mixing, make oral or external preparation; Perhaps, with water extracting liquid or water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder mixing, drying adds behind the enclose or the volatile oil of enclose not, makes oral or external preparation.
In the above-described preparation method technical scheme, the w/v of water that water is carried or alcohol extraction added or ethanol and raw material, extraction time, extraction time etc. can be with reference to common process.
Below be the drug action experiment that the inventor did:
One, experiment medicine
1, F1: prescription is that Rhizoma Alpiniae Officinarum, Flos Caryophylli, Radix Angelicae Sinensis, Radix Schefflerae Arboricolae, Pterospermi Heterophylli, weight proportion are 1: 1: 1: 8: 8;
2, F2: prescription is that Rhizoma Alpiniae Officinarum, Flos Caryophylli, Radix Angelicae Sinensis, Radix Schefflerae Arboricolae, weight proportion are 1: 1: 1: 16;
3, F3: prescription is that Rhizoma Alpiniae Officinarum, Flos Caryophylli, Radix Angelicae Sinensis, Pterospermi Heterophylli, weight proportion are 1: 1: 1: 16;
Two, experimentation and result
1, F1, F2, the research of F3 arthritis
1.1F1, F2, F3 be to the tentative arthritic influence of rabbit
Choose 36 of livid purple blue rabbit, body weight 1.8~2.2kg, male, adaptability formally begins test after raising a week, guarantees 20~25 ℃ of feeding environments in the feeding process, and humidity is 40~70%.Modeling was organized as blank according to 6 rabbit of rabbit body weight picked at random the same day, all the other rabbit begin sensitization, selected 5 injection points of being between the rabbit back scapula, the emulsifying agent 0.2ml/ point that forms by 3% egg protein solution and equal-volume incomplete Freund's adjuvant mixed configuration of subcutaneous injection respectively.Rabbit is normally raised after the sensitization, only injects 3% egg protein solution 0.2ml/ after 28 days in the rabbit right knee joint, carries out immune attack.
Behind the immune attack 2 months, according to rabbit arthroncus degree modeling group rabbit is divided into 5 groups at random: i.e. model group (NS, 2.5ml/kg), F1 organizes (0.625g/kg, 2.5ml/kg), F2 organizes (0.625g/kg, 2.5ml/kg), F3 organizes (0.625g/kg, 2.5ml/kg) and the ZHENGQINGFENGTONGNING group (0.02g/kg, 2.5ml/kg), grouping began administration the same day.Administering mode is a gastric infusion, once a day, and successive administration 3 months.
Each organized the rabbit successive administration after 3 months, got right knee joint and fixed with 10% formalin solution, did the routine pathology inspection.
Morphologic detection result is as seen: the movable no abnormality seen of rabbit after the sensitization, and second day is that visible attack joints has significant swelling behind the immune attack, and the swelling degree peaked about one week, and swelling degree is afterwards subdued gently gradually.The rabbit joint begins Secondary cases swelling after two months, compares with model group, and each all can reduce rabbit joint Secondary cases swelling degree after organizing administration to a certain extent.
Histological examination: do not see in the normal group articular cavity that inflammatory cell oozes out, synovial tissue is normal, and synovial cell's queueing discipline is not seen the lymphocyte nuclear monocyte infiltration, does not see proliferation of fibrous tissue; Inflammatory cell oozes out in the model group articular cavity, and articular surface is coarse, corrode, articular cartilage and subchondral bone disorganization, the cartilage surface pannus forms, the synovial cell becomes multilamellar, under the synovial membrane based on the inflammatory cell infiltration of lymphocyte; Each administration group can reduce inflammatory cell oozes out, and articular cartilage is destroyed and synovial membrane inflammation.
Each organizes the pathological score data with mean ± standard deviation
The statistical software with SPSS13.0 for windows is analyzed in expression, relatively uses one factor analysis of variance between group.Concrete pathological score statistical result such as following table 1.
Table 1 F1~F3 side is to the influence of rabbit experiment arthritis model joint pathology
Compare * * P<0.01 with model group
By last table result as can be seen: the model group pathological score has been compared significant difference (P<0.01) with the blank group, and the modeling success is described.F1, F2, F3 group and relatively all significantly reductions (P<0.01) of pathological score of model group illustrate that these three prescriptions all have therapeutical effect preferably to this rabbit arthritis model.
1.2F1, F2, F3 be to the influence of rat test osteoarthritis
All rat experiment chamber adaptabilities are raised and are begun grouping and modeling, raising condition: relative humidity 40~70%, 20~24 ℃ of temperature, the feed of freely drinking water after three days.
Adaptability is raised and is finished 10 rats of back picked at random as sham operated rats, and all the other 50 are adopted the Hulth method to set up rat knee joint OA model.With animal subject with 10% chloral hydrate 0.3ml/100g intraperitoneal injection of anesthesia after, lie on the back on the Rhizoma Atractylodis Macrocephalae platform, get the inboard longitudinal incision of right knee joint and be about 1cm, expose medial ligament and cut off, after open joint capsule, detect and cut off the front and back ligamentaum cruciatum after articular cavity does not have primary affection, complete excision medial meniscus, the layer-by-layer suture wound, aseptic wrapping is fixed, postoperative antibiotic (ampicillin) two days, prevention infection.
After two weeks, all modeling rats are equally divided into 5 groups at random: i.e. sham operated rats (NS, 20ml/kg), F1 group (1.625g/kg, 20ml/kg), F2 group (1.625g/kg, 20ml/kg), the F3 group (1.625g/kg, 20ml/kg) and ZHENGQINGFENGTONGNING group (0.05g/kg, 2.5ml/kg), divide into groups to begin administration the same day, once a day, be total to administration 30d.
Behind the last administration 1h, put to death animal, dissect the right side knee joint, cartilage downcuts that to put into 10% formalin solution fixing, uses for pathology detection.
Pathologic specimen routine pathology tissue slice, paraffin embedding, HE dyeing, microscopy.Articular cartilage tissue is learned Mankin ' the s method row pathological grading that adopts improvement, grade scale such as following table 2, and appraisal result is used for statistical analysis.
Table 2 articular cartilage tissue is learned Mankin ' grade form
Data are with mean ± standard deviation
The 13.0 for windows statistical softwares with SPSS are analyzed in expression, relatively use one factor analysis of variance between group.The results are shown in Table 3.
Micro-pathological observation: the arrangement of each layer of sham operated rats chondrocyte and substrate be distributed with certain rules, cells of superficial layer becomes fusiformis, becomes horizontally.The intermediate cell circle is dispersed in distribution, and the radiating layer chondrocyte becomes columnar arrangement.
There is significant depressions on model group cartilage top layer, and structure is difficult to differentiate, and serious top layer disappears, the part district is the fine hair shape, and visible more crack reaches radiating layer downwards deeply from the extension of top layer cartilage, and visible sparse knot is formed group and loose netted connection in the crack, the fiber filling is arranged, damp heading line off.
The coarse injustice in ZHENGQINGFENGTONGNING group top layer has the crack, and a bunch collection cell increases, indivedual chondrocyte irregular arrangement, disorder.
F1 group bone 4 stratum boundaries limit is unclear, but still can recognize, the chondrocyte rareness of top layer damaged part, and cell is arranged and is owed rule; F2 and the pathological change of F3 group are heavier slightly than F1, must protective effect but also shown.
Exposure is the fine hair shape, and the cartilage columnar arrangement disappears.Medication group part specimen chondrocyte is the nido hypertrophy.
Table 3 F1~F3 is to the influence of rat experiment arthritis knuckle synovial membrane pathological score
Compare * P<0.05, * * P<0.01 with model group
Learning Mankin ' s scoring according to articular cartilage tissue analyzes: model group increases obviously (P<0.01) with sham operated rats score value relatively, shows the model success.Three administration groups are compared score value with model group all have obvious decline (P<0.01), illustrates that F1, F2, three prescriptions of F3 can effectively improve the symptom of rat OA model.
2, F1, F2, the research of F3 analgesic activity.
2.1F1, F2, F3 cause the influence of mice vola pain to hot plate.
Choose female ICR mice, body weight 18~22g buys by oneself and tests after affording 3d.The mice sub-cage rearing, feeding environment temperature-controlled chamber temperature is 20~25 ℃, humidity is 40~70%, the ad lib water inlet.Get female mice, put into (55 ± 0.5) ℃ hot plate dolorimeter, to lick metapedes as the pain reaction index, pain threshold before the prediction mice medicine.Be considered as qualified mice less than 30s greater than 5s with pain threshold.50 mices that pain threshold is qualified are divided into 5 groups at random, every group 10, be blank group of group (NS, 20ml/kg), F1 group (3.125g/kg, 20ml/kg), F2 group (3.125g/kg, 20ml/kg), F3 group (3.125g/kg, 20ml/kg) and positive control (tramadol hydrochloride) organize (0.05g/kg, 20ml/kg).Administering mode is a gastric infusion, and be administered once every day, altogether administration 3d.1h after the last administration is placed on mice on the metallic plate of 55 ℃ of preheatings, constant temperature, and measure pain threshold as the threshold of pain index incubation period of licking metapedes reaction with mice.As if no pain reaction in the 60s, promptly take out immediately in the mensuration process, and calculate with 60s.
Data are with mean ± standard deviation
The statistical software with SPSS13.0 for windows is analyzed in expression, relatively uses one factor analysis of variance between group.The results are shown in Table 4.
Table 4 F1~F3 causes the influence of pain mice paroxysmal pain effect to hot plate method
Compare with model group: * * P<0.01
The result shows that each administration group mice pain threshold significantly increases (P<0.01) with blank group.Results suggest F1, F2, three prescriptions of F3 all have the effect that stronger heat resistanceheat resistant plate causes mice vola pain.
2.2 F1, F2, F3 Dichlorodiphenyl Acetate cause the influence of mouse writhing pain.
50 of the ICR mices of body weight 18~22g, male and female half and half divide the cage adaptability to raise 3, freely drink water, and raise with the laboratory animal mixed feed 20~23 ℃ of room temperatures, humidity 50~70%.Be divided into 5 groups at random, 10 every group.Promptly blank group (NS, 20ml/kg), the F1 group (3.125g/kg, 20ml/kg), the F2 group (3.125g/kg, 20ml/kg), the F3 group (3.125g/kg, 20ml/kg) and positive control (rotundine) organize (0.03g/kg, 20ml/kg).Administering mode is a gastric infusion, and be administered once every day, altogether administration 3d.
Positive control (rotundine) group, dosage is the 0.03g/kg body weight, the blank group gives isometric normal saline.Administering mode is a gastric infusion, and the administration volume is 20ml/kg, and be administered once every day, altogether administration 3d.
Last administration 1h respectively organizes mouse peritoneal and only injects 0.7% acetic acid normal saline solution 0.2ml/, use manual time-keeping simultaneously, occurring in time (being incubation period) of writhing response for the first time and the 15min appears in every mice of observed and recorded turns round the body number of times, carries out statistical analysis.
Statistical result with
Expression adopts the one factor analysis of variance of SPSS 13.0 to add up.Difference between relatively each is organized.The results are shown in Table 5.
Table 5 F1~F3 Dichlorodiphenyl Acetate causes the influence of mouse writhing reaction analgesic activity
Compare with the blank group: * * P<0.01
The result shows: medicine F1, F2 and F3 all can significantly reduce the mouse writhing number of times of acetic-acid induced, have compared significant difference (P<0.01) with model group.Illustrate that each group of F1, F2 and F3 all has the effect that stronger anti-acetic acid causes mouse writhing pain.
3, F1, F2, the research of F3 antiinflammatory action
3.1F1, F2, F3 be to the granulomatous influence of rat button
Get 50 of SD rats, male and female half and half, body weight 130~170g.Sub-cage rearing, ad lib drinking-water is raised with the laboratory animal mixed feed, keeps 20~25 ℃ of room temperatures, humidity 40~70%.Formally begin test behind the laboratory compatibility test 3d, rat is divided into 5 groups at random, be respectively: model group (NS, 20ml/kg), F1 group (1.625g/kg, 20ml/kg), F2 group (1.625g/kg, 20ml/kg), F3 group (1.625g/kg, 20ml/kg) and positive control (dexamethasone) organize (0.01g/kg, 20ml/kg).Each is organized rat and uses the ether light anaesthesia, wipes out the rat chesk hair, and iodine disinfection cuts skin of chest, fills in each one on button from otch respectively to two forelimb oxters, and experiment guarantees size and degree of roughness unanimity with button through the gauze polishing.Skin suture, intramuscular injection penicillin and streptomycin infection.Begin to irritate stomach behind the postoperative 6d and give relative medicine, every day 1 time, continuous 10 days, rat is put to death in the cervical vertebra dislocation behind last administration 24h, peels off the button granulation tissue, dry 24h in 60 ℃ of baking ovens, the electronic balance precision is weighed (1/1000g), calculates granuloma weight, respectively organizes difference.
Data are with mean ± standard deviation
The statistical software with SPSS13.0 for windows is analyzed in expression, relatively uses one factor analysis of variance between group.The results are shown in Table 6.
Table 6 F1~F3 is to the granulomatous influence of rat button
* represent to compare with model group P<0.05, * * represents to compare with model group P<0.01
The result shows: compare with model group, F1~F3 group can significantly reduce the granulomatous dry weight of rat button (P<0.01, P<0.05).Illustrate that each administration group of F1~F3 all has the granulomatous effect of the rat button of inhibition.
3.2 F1, F2, F3 Dichlorodiphenyl Acetate cause the influence that the mouse peritoneal capillary permeability increases.
Get 50 of ICR mices, male, body weight 18~22g.Mice is divided equally the cage raising, freely drinks water, and raises with the laboratory animal mixed feed, keeps 20~25 ℃ of room temperatures, humidity 40~70%.The mice adaptability is fed and is begun test after three days.Be divided into 5 groups at random, 10 every group.Be respectively: promptly blank group (NS, 20ml/kg), the F1 group (3.125g/kg, 20ml/kg), the F2 group (3.125g/kg, 20ml/kg), the F3 group (3.125g/kg, 20ml/kg) and positive control (aspirin) organize (0.2g/kg, 20ml/kg).Administering mode is a gastric infusion, and be administered once every day, altogether administration 3d.
30min after the last administration, each organizes mouse mainline 0.5% azovan blue normal saline solution 0.1ml/10g, lumbar injection 0.6% acetic acid 0.2ml/ only immediately, put to death behind the 20min, cut off skin of abdomen muscle immediately, with 5ml normal saline washing abdominal cavity, the sucking-off cleaning mixture, 3000 leave heart 15min, get one times of supernatant dilution, measure each sample cell absorbance in the 590nm place.
Data are with mean ± standard deviation
The statistical software with SPSS13.0 for windows is analyzed in expression, relatively uses one factor analysis of variance between group.The results are shown in Table 7.
Table 7 F1~F3 Dichlorodiphenyl Acetate causes the influence that the mouse peritoneal capillary permeability increases
* represents to compare with model group P<0.01
As can be seen from the above table, the absorbance of each administration group mouse peritoneal transudate is starkly lower than model control group (P<0.01).It can be said that three prescriptions of bright F1~F3 all can resist acetic acid to a certain extent and cause the effect that the mouse peritoneal permeability increases, have antiphlogistic effects.
3.3 F1, F2, F3 are to the influence of mice auricle swelling
Get 50 of normal ICR mices, body weight 25~30g, male.Mus is divided equally the cage raising, freely drinks water, and raises with the laboratory animal mixed feed, keeps 20~25 ℃ of room temperatures, humidity 40~70%.The mice adaptability is fed and is begun test after three days.Be divided into 5 groups at random, 10 every group.Be respectively: blank group (NS, 20ml/kg), the F1 group (3.125g/kg, 20ml/kg), the F2 group (3.125g/kg, 20ml/kg), the F3 group (3.125g/kg, 20ml/kg) and positive control (aspirin) organize (0.2g/kg, 20ml/kg).Administering mode is a gastric infusion, and be administered once every day, altogether administration 3d.
Respectively organize mice behind the last administration 40min and be applied to two sides, ear front and back, a mice left side with 2% Oleum Tiglii 0.05ml respectively, after causing scorching 4h, put to death mice, two ears about cutting along the auricle baseline, (diameter 9mm) takes off round auricle at same position respectively with card punch, scales/electronic balance weighing, as auricle swelling degree, and calculate the swelling percentage rate with the difference of mice left and right sides auricle weight.
Data are with mean ± standard deviation
The statistical software with SPSS13.0 for windows is analyzed in expression, relatively uses one factor analysis of variance between group.The results are shown in Table 8.
Table 8 F1~F3 is to the influence of Oleum Tiglii induced mice ear swelling
Compare * p<0.05, * * p<0.01 with the blank group
As can be seen from the above table: F1, F2, F3 group mice auricle swelling degree are compared the reduction (P<0.05, P<0.01) that has in various degree after the administration with model group.Can draw three prescriptions of F1~F3 thus and show the swollen inflammatory effect of certain anti-Mus ear.
The inventor has filtered out antalgic and inflammation relieving obvious results Chinese medicine preparation by going deep into systematic study.Compared with prior art, pharmaceutical composition of the present invention has good safety, remarkable antalgic and inflammation relieving, obviously improve the various symptoms that Chinese medical discrimination belongs to the arthralgia due to wind-cold-dampness disease patient, be applicable to the treatment of rheumatic arthritis, rheumatoid arthritis, numb limbs and tense tendons, back cold type of pain, scapulohumeral periarthritis, neck-shoulder pain, lumbago and leg pain, sciatica, trigeminal neuralgia, hyperosteogeny, prolapse of lumbar intervertebral disc, postpartum apoplexy etc.
The specific embodiment
Below further describe concrete technical scheme of the present invention,, and do not constitute restriction its right so that those skilled in the art understands the present invention further.
Embodiment 1.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 0.1; Flos Caryophylli 0.1; Radix Angelicae Sinensis 0.1; Radix Schefflerae Arboricolae 0.6; Pterospermi Heterophylli 0.8.Technology extracts each materials of effective ingredients routinely, adds conventional pharmaceutic adjuvant, makes oral or external preparation.
Embodiment 2.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 0.1; Flos Caryophylli 0.1; Radix Angelicae Sinensis 0.1; Radix Schefflerae Arboricolae 30; Pterospermi Heterophylli 30.Its preparation method is to get whole raw material of Chinese medicine by weight ratio to pulverize, and makes powder or honeyed pill.
Embodiment 3.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 1; Flos Caryophylli 1; Radix Angelicae Sinensis 1; Radix Schefflerae Arboricolae 10; Pterospermi Heterophylli 10.Its preparation method is: get whole raw material of Chinese medicine by weight ratio and carry out decocting and get the water extract, the water extract is made oral or external preparation after concentrating.
Embodiment 4.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 2; Flos Caryophylli 1; Radix Angelicae Sinensis 3; Radix Schefflerae Arboricolae 20.Its preparation method is: get whole raw material of Chinese medicine by weight ratio and carry out decocting and get the water extract, the water extract concentrates the back precipitate with ethanol, and drying is made capsule after the precipitate with ethanol supernatant concentration.
Embodiment 5.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 2; Flos Caryophylli 2; Radix Angelicae Sinensis 2; Pterospermi Heterophylli 18.Its preparation method is: get whole raw material of Chinese medicine by weight ratio and carry out alcohol extraction, alcohol extract is made oral or external preparation after concentrating, and perhaps concentrates the back drying and makes external preparation.
Embodiment 6.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 4; Flos Caryophylli 3; Radix Angelicae Sinensis 6; Pterospermi Heterophylli 12.Its preparation method is: get whole raw material of Chinese medicine by weight ratio and carry out alcohol extraction, alcohol extract concentrates the back water precipitating, and the water precipitating supernatant concentration is made soft capsule, and perhaps drying is made capsule.
Embodiment 7.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 6; Flos Caryophylli 6; Radix Angelicae Sinensis 6; Radix Schefflerae Arboricolae 6; Pterospermi Heterophylli 6.Its preparation method is: get Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil by weight ratio; Medicinal residues merge alcohol extraction with the residue raw material of Chinese medicine, and alcohol extract concentrates, and adds volatile oil, makes oral soft capsule.
Embodiment 8.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 1.5; Flos Caryophylli 1.5; Radix Angelicae Sinensis 1.5; Radix Schefflerae Arboricolae 15.Its preparation method is: get Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil by weight ratio; Medicinal residues merge alcohol extraction with the residue raw material of Chinese medicine, and the alcohol extract concentrate drying adds behind the enclose or the volatile oil of enclose not, makes capsule.
Embodiment 9.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 2; Flos Caryophylli 2; Radix Angelicae Sinensis 2; Radix Schefflerae Arboricolae 2; Pterospermi Heterophylli 2.Its preparation method is: get whole raw material of Chinese medicine by weight ratio and carry out alcohol extraction, alcohol extract concentrates; The medicinal residues decocting, the water extract concentrates; Above-mentioned alcohol extraction concentrated solution, water extracting liquid are merged, make externally applied ointment.
Embodiment 10.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 1; Flos Caryophylli 1; Radix Angelicae Sinensis 1; Radix Schefflerae Arboricolae 2; Pterospermi Heterophylli 8.Its preparation method is: get Flos Caryophylli, Radix Angelicae Sinensis 2 flavor extraction volatile oil by weight ratio; Medicinal residues merge decocting with the residue raw material of Chinese medicine, with water extract concentrate drying, add behind the enclose or the volatile oil of enclose not, make oral or external preparation.
Embodiment 11.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 2; Flos Caryophylli 1; Radix Angelicae Sinensis 3; Radix Schefflerae Arboricolae 8; Pterospermi Heterophylli 2.Its preparation method is: get Flos Caryophylli, Rhizoma Alpiniae Officinarum 2 flavor extraction volatile oil by weight ratio; Medicinal residues merge water with the residue raw material of Chinese medicine to be carried, and the water extract concentrates the back precipitate with ethanol, and the precipitate with ethanol supernatant liquid that contracts adds volatile oil, makes oral or external preparation;
Embodiment 12.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 2; Flos Caryophylli 1; Radix Angelicae Sinensis 3; Pterospermi Heterophylli 10.Its preparation method is: get Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil by weight ratio; Medicinal residues merge alcohol extraction with the residue raw material of Chinese medicine, and alcohol extract concentrates; The medicinal residues decocting, the water extract concentrates; With water extracting liquid, alcohol extraction concentrated solution drying, add behind the enclose or the volatile oil of enclose not, make oral or external preparation.
Embodiment 13.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 2; Flos Caryophylli 1; Radix Angelicae Sinensis 3; Pterospermi Heterophylli 10.Its preparation method is: get Pterospermi Heterophylli water by weight ratio and carry, the water extract concentrates, precipitate with ethanol, precipitate with ethanol supernatant concentration; Get Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil by weight ratio, the medicinal residues alcohol extraction, alcohol extract concentrates; Water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution and volatile oil are merged, make oral or external preparation; Perhaps,, add behind the enclose or the volatile oil of enclose not, make oral or external preparation water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution drying.
Embodiment 14.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 3; Flos Caryophylli 1; Radix Angelicae Sinensis 6; Radix Schefflerae Arboricolae 8; Pterospermi Heterophylli 12.Its preparation method is: get Radix Schefflerae Arboricolae, Pterospermi Heterophylli 2 flavor water and carry, the water extract concentrates; Get Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil by weight ratio, the medicinal residues alcohol extraction, alcohol extract concentrates; Water extracting liquid, alcohol extraction concentrated solution and volatile oil are merged, make oral or external preparation; Perhaps,, add behind the enclose or the volatile oil of enclose not, make oral or external preparation water extracting liquid, alcohol extraction concentrated solution drying.
Embodiment 15.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 6; Flos Caryophylli 3; Radix Angelicae Sinensis 6; Radix Schefflerae Arboricolae 15.Its preparation method is: get the Radix Schefflerae Arboricolae alcohol extraction by weight ratio, alcohol extract concentrates; Get Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil, medicinal residues water is carried, and the water extract concentrates; Water extracting liquid, alcohol extraction concentrated solution and volatile oil are merged, make oral or external preparation; Perhaps,, add behind the enclose or the volatile oil of enclose not, make oral or external preparation water extracting liquid, alcohol extraction concentrated solution drying.
Embodiment 16.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 2; Flos Caryophylli 1; Radix Angelicae Sinensis 8; Radix Schefflerae Arboricolae 9.Its preparation method is: get the Radix Schefflerae Arboricolae alcohol extraction by weight ratio, alcohol extract concentrates water precipitating, the water precipitating supernatant concentration; Get Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil, medicinal residues water is carried, and the water extract concentrates; Water extracting liquid, alcohol extracting-water precipitating concentrated solution and volatile oil are merged, make oral or external preparation; Perhaps,, add behind the enclose or the volatile oil of enclose not, make oral or external preparation water extracting liquid, alcohol extracting-water precipitating concentrated solution drying.
Embodiment 17.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 3; Flos Caryophylli 1; Radix Angelicae Sinensis 1; Radix Schefflerae Arboricolae 6.Its preparation method is: get Radix Schefflerae Arboricolae water by weight ratio and carry, the water extract concentrates; The alcohol extraction of residue raw material of Chinese medicine, alcohol extract concentrates; Water extracting liquid, alcohol extraction concentrated solution are merged, make oral or external preparation; Perhaps, with water extracting liquid, alcohol extraction concentrated solution drying, make oral or external preparation.
Embodiment 18.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 0.9; Flos Caryophylli 0.9; Radix Angelicae Sinensis 0.9; Radix Schefflerae Arboricolae 3; Pterospermi Heterophylli 3.Its preparation method is: get whole raw material of Chinese medicine by weight ratio and pulverize, make oral or external preparation.
Embodiment 19.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 3; Flos Caryophylli 3; Radix Angelicae Sinensis 3; Radix Schefflerae Arboricolae 6; Pterospermi Heterophylli 6.Its preparation method is: get Flos Caryophylli, Rhizoma Alpiniae Officinarum, the whole powder of beating of Radix Angelicae Sinensis 3 flavors by weight ratio; The residue raw material of Chinese medicine merges alcohol extraction, and alcohol extract concentrates back and above-mentioned powder mixing, makes oral or external preparation.
Embodiment 20.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 3; Flos Caryophylli 3; Radix Angelicae Sinensis 3; Radix Schefflerae Arboricolae 2; Pterospermi Heterophylli 2.Its preparation method is: get Flos Caryophylli, the whole powder of beating of Rhizoma Alpiniae Officinarum 2 flavors by weight ratio; Residue raw material of Chinese medicine water is together carried, and the water extract concentrates; With water extracting liquid and above-mentioned medicated powder mixing, make oral or external preparation.Perhaps, with water extracting liquid and above-mentioned medicated powder mixing, drying is made oral or external preparation.
Embodiment 21.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 1.5; Flos Caryophylli 1.5; Radix Angelicae Sinensis 1.5; Pterospermi Heterophylli 5.Its preparation method is: get Pterospermi Heterophylli by weight ratio and all beat powder; Get Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil by weight ratio, the medicinal residues alcohol extraction; With alcohol extraction concentrated solution, above-mentioned medicated powder and volatile oil mixing, make oral or external preparation; Perhaps, with alcohol extraction concentrated solution, above-mentioned medicated powder mixing, drying adds behind the enclose or the volatile oil of enclose not, makes oral or external preparation.
Embodiment 22.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 15; Flos Caryophylli 15; Radix Angelicae Sinensis 15; Pterospermi Heterophylli 30.Its preparation method is: by weight ratio half got Pterospermi Heterophylli and beaten powder; Get Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis 3 flavor extraction volatile oil by weight ratio; Medicinal residues merge water with the residue raw material of Chinese medicine to be carried, and the water extract concentrates precipitate with ethanol, precipitate with ethanol supernatant concentration; With water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder and volatile oil mixing, make oral or external preparation; Perhaps, with water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder mixing, drying adds behind the enclose or the volatile oil of enclose not, makes oral or external preparation.
Embodiment 23.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 15; Flos Caryophylli 15; Radix Angelicae Sinensis 15; Radix Schefflerae Arboricolae 30; Pterospermi Heterophylli 30.Its preparation method is: get whole raw material of Chinese medicine by weight ratio and carry out decocting and get the water extract; The water extract is made oral or external preparation after concentrating.
Embodiment 24.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 0.5; Flos Caryophylli 0.5; Radix Angelicae Sinensis 0.5; Radix Schefflerae Arboricolae 0.5; Pterospermi Heterophylli 0.5.Its preparation method is: get whole raw material of Chinese medicine by weight ratio and carry out decocting and get the water extract, the water intaking extract concentrates the back precipitate with ethanol, the precipitate with ethanol supernatant; Make oral or external preparation after the precipitate with ethanol supernatant concentration.
Embodiment 25.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 0.5; Flos Caryophylli 0.5; Radix Angelicae Sinensis 0.5; Radix Schefflerae Arboricolae 0.5; Pterospermi Heterophylli 0.5.Its preparation method is: get whole raw material of Chinese medicine by weight ratio and carry out decocting and get the water extract; The water intaking extract concentrates the back drying and makes oral or external preparation.
Embodiment 26.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 0.1; Flos Caryophylli 0.1; Radix Angelicae Sinensis 0.1; Its preparation method is: get whole raw material of Chinese medicine by weight ratio and carry out decocting and get the water extract; The water intaking extract concentrates the back drying and makes oral or external preparation.
Embodiment 27.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 6; Flos Caryophylli 3; Radix Angelicae Sinensis 9; Radix Schefflerae Arboricolae 20; Pterospermi Heterophylli 20.Its preparation method is: get whole raw material of Chinese medicine by weight ratio and carry out decocting and get the water extract, the water intaking extract concentrates the back precipitate with ethanol, the precipitate with ethanol supernatant; Drying is made oral or external preparation after the precipitate with ethanol supernatant concentration.
Embodiment 28.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 6; Flos Caryophylli 3; Radix Angelicae Sinensis 9; Radix Schefflerae Arboricolae 0.5; Pterospermi Heterophylli 0.5.Its preparation method is: get whole raw material of Chinese medicine by weight ratio and carry out alcohol extraction, alcohol extract concentrate the alcohol extraction concentrated solution; Perhaps, alcohol extraction concentrated solution water precipitating, the water precipitating supernatant concentration gets the alcohol extracting-water precipitating concentrated solution; Make oral alcohol extraction concentrated solution or alcohol extracting-water precipitating concentrated solution or external preparation; Perhaps, with alcohol extraction concentrated solution or alcohol extracting-water precipitating concentrated solution drying, make oral or external preparation.
Embodiment 29.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 0.5; Flos Caryophylli 0.5; Radix Angelicae Sinensis 0.5; Radix Schefflerae Arboricolae 20; Pterospermi Heterophylli 20.Its preparation method is: get flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, the Radix Angelicae Sinensis or 2 flavors or 3 flavors by weight ratio and extract volatile oil; Medicinal residues merge alcohol extraction with the residue raw material of Chinese medicine, and alcohol extract concentrates, and adds volatile oil, makes oral or external preparation; Perhaps with the alcohol extract concentrate drying, add behind the enclose or the volatile oil of enclose not, make oral or external preparation.
Embodiment 30.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 0.1; Flos Caryophylli 0.1; Radix Angelicae Sinensis 0.1; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 30; Its preparation method is: get whole raw material of Chinese medicine by weight ratio and carry out alcohol extraction, alcohol extract concentrates; The medicinal residues decocting, the water extract concentrates; Above-mentioned alcohol extraction concentrated solution, water extracting liquid are merged, make oral or external preparation; Perhaps, with alcohol extraction concentrated solution, water extracting liquid drying, make oral or external preparation.
Embodiment 31.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 15; Flos Caryophylli 15; Radix Angelicae Sinensis 15; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 0.5.Its preparation method is: get flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, the Radix Angelicae Sinensis or 2 flavors or 3 flavors by weight ratio and extract volatile oil; Medicinal residues merge decocting with the residue raw material of Chinese medicine, the water extract concentrate water extracting liquid; Perhaps, the water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration gets the water extract-alcohol precipitation concentrated solution; Get water extracting liquid or water extract-alcohol precipitation concentrated solution, add volatile oil, make oral or external preparation; Perhaps,, add behind the enclose or the volatile oil of enclose not, make oral or external preparation water extracting liquid or water extract-alcohol precipitation concentrated solution drying.
Embodiment 32.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 0.5; Flos Caryophylli 0.5; Radix Angelicae Sinensis 0.5; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 20.Its preparation method is:
(1) gets flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, the Radix Angelicae Sinensis or 2 flavors or 3 flavors by weight ratio and extract volatile oil;
(2) medicinal residues merge alcohol extraction with the residue raw material of Chinese medicine, and alcohol extract concentrates;
(3) medicinal residues decocting, water extract concentrate water extracting liquid; Perhaps, the water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration gets the water extract-alcohol precipitation concentrated solution;
(4) water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution and volatile oil are merged, make oral or external preparation; Perhaps,, add behind the enclose or the volatile oil of enclose not, make oral or external preparation water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution drying.
Embodiment 33.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 6; Flos Caryophylli 3; Radix Angelicae Sinensis 9; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 0.5.Its preparation method is:
(1) get 1 in Radix Schefflerae Arboricolae, Pterospermi Heterophylli flavor by weight ratio or 2 flavor water are carried, the water extract concentrate water extracting liquid; Perhaps water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration, the water extract-alcohol precipitation concentrated solution;
(2) get flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, the Radix Angelicae Sinensis or 2 flavors or 3 flavors by weight ratio and extract volatile oil, the medicinal residues alcohol extraction, alcohol extract concentrates;
(3) water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution and volatile oil are merged, make oral or external preparation; Perhaps,, add behind the enclose or the volatile oil of enclose not, make oral or external preparation water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution drying.
Embodiment 34.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 0.5; Flos Caryophylli 0.5; Radix Angelicae Sinensis 0.5; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 0.5.
Its preparation method is:
(1) get 1 in Radix Schefflerae Arboricolae, Pterospermi Heterophylli flavor or 2 flavor alcohol extractions by weight ratio, alcohol extract concentrate the alcohol extraction concentrated solution; Perhaps, alcohol extraction concentrated solution water precipitating, the water precipitating supernatant concentration gets the alcohol extracting-water precipitating concentrated solution;
(2) get 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, Radix Angelicae Sinensis flavor or 2 flavors or 3 flavors by weight ratio and extract volatile oil, medicinal residues water is carried, the water extract concentrate water extracting liquid; Perhaps water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration, the water extract-alcohol precipitation concentrated solution;
(3) with above-mentioned alcohol extraction concentrated solution or alcohol extracting-water precipitating concentrated solution, water extracting liquid or water extract-alcohol precipitation concentrated solution and volatile oil merging, make oral or external preparation; Perhaps,, add behind the enclose or the volatile oil of enclose not, make oral or external preparation alcohol extraction concentrated solution or alcohol extracting-water precipitating concentrated solution, water extracting liquid or water extract-alcohol precipitation concentrated solution drying.
Embodiment 35.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 6; Flos Caryophylli 3; Radix Angelicae Sinensis 9; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 20.Its preparation method is:
(1) get 1 in Radix Schefflerae Arboricolae, Pterospermi Heterophylli flavor by weight ratio or 2 flavor water are carried, the water extract concentrate water extracting liquid; Perhaps water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration, the water extract-alcohol precipitation concentrated solution;
(2) residue raw material of Chinese medicine alcohol extraction, alcohol extract concentrates;
(3) water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution are merged, make oral or external preparation; Perhaps, with water extracting liquid or water extract-alcohol precipitation concentrated solution, alcohol extraction concentrated solution drying, make oral or external preparation.
Embodiment 36.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 4; Flos Caryophylli 4; Radix Angelicae Sinensis 10; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 15.Its preparation method is: get whole raw material of Chinese medicine by weight ratio and pulverize, make oral or external preparation.
Embodiment 37.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 15; Flos Caryophylli 15; Radix Angelicae Sinensis 15; Its preparation method is: get whole raw material of Chinese medicine by weight ratio and pulverize, make oral or external preparation.
Embodiment 38.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 4; Flos Caryophylli 4; Radix Angelicae Sinensis 10; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 15.Its preparation method is:
(1) gets flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, the Radix Angelicae Sinensis or 2 flavors or all or part of powder of beating of 3 flavors by weight ratio;
(2) residue raw material of Chinese medicine alcohol extraction together, alcohol extract concentrates back and above-mentioned powder mixing, makes oral or external preparation.
Embodiment 39.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 10; Flos Caryophylli 8; Radix Angelicae Sinensis 15; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 25.Its preparation method is:
(1) gets flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, the Radix Angelicae Sinensis or 2 flavors or all or part of powder of beating of 3 flavors by weight ratio;
(2) the residue raw material of Chinese medicine together water carry, the water extract concentrate water extracting liquid; Perhaps water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration, the water extract-alcohol precipitation concentrated solution;
(3) with water extracting liquid or water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder mixing, make oral or external preparation.Perhaps, with water extracting liquid or water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder mixing, drying is made oral or external preparation.
Embodiment 40.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 2; Flos Caryophylli 12; Radix Angelicae Sinensis 10; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 18.Its preparation method is:
(1) gets all or part of powder of beating of Radix Schefflerae Arboricolae or Pterospermi Heterophylli by weight ratio;
(2) get flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, the Radix Angelicae Sinensis or 2 flavors or 3 flavors by weight ratio and extract volatile oil; Medicinal residues merge alcohol extraction with the residue raw material of Chinese medicine, and alcohol extract concentrates;
(3) with alcohol extraction concentrated solution, above-mentioned medicated powder and volatile oil mixing, make oral or external preparation; Perhaps, with alcohol extraction concentrated solution, above-mentioned medicated powder mixing, drying adds behind the enclose or the volatile oil of enclose not, makes oral or external preparation.
Embodiment 41.The pharmaceutical composition of a kind of expelling wind and removing dampness, activating collaterals to relieve pain, it is to be made by following Chinese medicine raw materials by weight proportion, Rhizoma Alpiniae Officinarum 0.5; Flos Caryophylli 1; Radix Angelicae Sinensis 0.8; Radix Schefflerae Arboricolae or Pterospermi Heterophylli 15.Its preparation method is:
(1) gets all or part of powder of beating of Radix Schefflerae Arboricolae or Pterospermi Heterophylli by weight ratio;
(2) get flavor of 1 in Flos Caryophylli, Rhizoma Alpiniae Officinarum, the Radix Angelicae Sinensis or 2 flavors or 3 flavors by weight ratio and extract volatile oil; Medicinal residues merge water with the residue raw material of Chinese medicine to be carried, the water extract concentrate water extracting liquid; Perhaps water extracting liquid precipitate with ethanol, the precipitate with ethanol supernatant concentration, the water extract-alcohol precipitation concentrated solution;
(3) with water extracting liquid or water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder and volatile oil mixing, make oral or external preparation; Perhaps, with water extracting liquid or water extract-alcohol precipitation concentrated solution and above-mentioned medicated powder mixing, drying adds behind the enclose or the volatile oil of enclose not, makes oral or external preparation.