CN101878782B - SF-1 water-soluble biological seed coating for preventing and curing maize head smut - Google Patents
SF-1 water-soluble biological seed coating for preventing and curing maize head smut Download PDFInfo
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- CN101878782B CN101878782B CN2010102365365A CN201010236536A CN101878782B CN 101878782 B CN101878782 B CN 101878782B CN 2010102365365 A CN2010102365365 A CN 2010102365365A CN 201010236536 A CN201010236536 A CN 201010236536A CN 101878782 B CN101878782 B CN 101878782B
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Abstract
The invention discloses an SF-1 water-soluble biological seed coating for preventing and curing maize head smut and relates to a biological seed coating for preventing and curing maize head smut. The invention solves the problems that the chemical preparation for preventing and curing the maize head smut has pollution to the environment and high residue and the germs easily become resistant to the chemicals. The SF-1 water-soluble biological seed coating for preventing and curing the maize head smut is composed of metabolic product of strains SF-1, farm lysozym, zinc sulfate, boric acid, polyoxyethylene, polyethylene glycol, water-soluble eosin, NaCl, ethylene glycol and the balance of distilled water. The biological seed coating of the invention can effectively prevent and cure the maize head smut; the resistance ability of the crops is strengthened; the prevention and cure effect reaches 86.5% to 86.82%; the yield increasing rate of the maize reaches 13.69% to 14.28%; in addition, the invention is beneficial to the growth of the underground part of the maize plants, promotion of the root growth, promotion of the growth and development of the entire plants, no influence to the safety of the maize, no pollution to the environment, no residual and difficulty of generating resistance to the chemicals.
Description
Technical field
The present invention relates to the biological seed coating agent of control of maize head smut.
Background technology
Maize head smut (Sporisorium reiianum (kuhn.) langdon Fuiierton) is the important disease in Chinese spring sowing corn district, in the Northeast China, ground such as North China, northwest, southwest and south China generally takes place.This disease is the systemic infection venereal disease evil of invading the corn germination phase, once morbidity, at first destroys female fringe, and the incidence of disease equals percent of loss, has caused very big loss to Maize Production.The main at present chemical agent (chemical composition is prepared into kind of a clothing agent) that adopts comes control of maize head smut evil; But chemical agent exists contaminated environment; Residual height; Germ is easy to generate problems such as pesticide resistance, runs counter to the green agriculture of present promotion, therefore selects suitable biological prevention and control agent to come the control of maize head smut extremely urgent.
Summary of the invention
The objective of the invention is to adopt chemical agent to come the control of maize head smut to exist contaminated environment, residual height, germ to be easy to generate drug-fast problem, and the SF-1 water-soluble biological seed coating agent of control of maize head smut is provided in order to solve.
The SF-1 water-soluble biological seed coating agent of control of maize head smut be every 100mL by volume portion rate form by the distilled water of the metabolite of 18～22 parts of bacterial strain SF-1,2～5 parts of agricultural enzyme microorganisms (BYM), 0.02～0.04 part of zinc sulphate, 0.01～0.02 part of boric acid, 2.5 parts of polyoxyethylene, 2 parts of polyethylene glycol, 1～2 part of water-soluble Yihong, 4 parts of NaCl, 5 parts of ethylene glycol and surplus; Wherein bacterial strain SF-1 is Jia Dena streptomycete (Streptomyces gardneri).
The SF-1 water-soluble biological seed coating agent of control of maize head smut among the present invention; Be mainly used in the preceding seed pelleting of corn seeding; Control of maize head smut effectively, enhancing crop anti-adversity ability, control efficiency reach 86.5%～86.82%, the rate of growth of corn reaches 13.69%～14.28%.
The corn seed that the SF-1 water-soluble biological seed coating agent of control of maize head smut was handled among the present invention is compared with untreated corn seed, and the former helps the growth of milpa under ground portion, increases fibrous root, improves root/shoot ratio; Promote root growth; Promote growing of whole strain plant, safety does not have influence to corn, and does not pollute the environment; Noresidue is difficult for developing immunity to drugs.
Embodiment
Technical scheme of the present invention is not limited to following cited embodiment, also comprises the combination in any between each embodiment.
Embodiment one: the SF-1 water-soluble biological seed coating agent of this embodiment control of maize head smut be every 100mL by volume portion rate form by the distilled water of the metabolite of 18～22 parts of bacterial strain SF-1,2～5 parts of agricultural enzyme microorganisms (BYM), 0.02～0.04 part of zinc sulphate, 0.01～0.02 part of boric acid, 2.5 parts of polyoxyethylene, 2 parts of polyethylene glycol, 1～2 part of water-soluble Yihong, 4 parts of NaCl, 5 parts of ethylene glycol and surplus; Wherein bacterial strain SF-1 is Jia Dena streptomycete Streptomyces gardneri.
Use as warning colouration in water-soluble Yihong in this embodiment.
Bacterial strain SF-1 is Jia Dena streptomycete Streptomyces gardneri in this embodiment; Be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center; The preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City; Institute of Microorganism, Academia Sinica, preservation date is on 06 17th, 2010, deposit number is CGMCC3928.
The morphological feature of bacterial strain SF-1 in this embodiment: SF-1 Gram-positive; After growing 7 days on the medium such as GYM agar and yeast starch agar and oat meal agar, substrate mycelium physically well develops, and no tabula does not rupture; Aerial hyphae well-grown, multiple-limb; Produce gentle bent extremely loose spiral fibrillae of spores, spore sphere to oval, smooth.
The physiological and biochemical property of bacterial strain SF-1 in this embodiment: glucose test is positive; The arabinose test is positive; The galactose test is positive; Cane sugar test is positive; The raffinose test is positive; The rhamnose test is positive; Fructose test is positive; The mannose test is negative; Lactose test is positive; The inositol test is negative; Gelatin liquefaction test is positive; The starch hydrolysis experiment is positive; Nitrate reduction test is negative; It is positive that class melanin produces test; The cellulose degradation test is negative; The milk setting test is negative; The blancmange test is negative; H
2S generation test is positive, tyrosine degraded test is positive.
The result that correlated series Blast compares among the 16S rDNA sequence of bacterial strain SF-1 and the GenBank in this embodiment shows that bacterial strain SF-1 belongs to streptomyces; Compare with the relevant bacterial strain of delivering at present, the sequence similarity of the 16S rDNA sequence of bacterial strain and Jia Dena streptomycete Streptomyces gardneri is very high, is 99.7%.
The metabolite of bacterial strain SF-1 is to the mensuration of corn safety in this embodiment: through seed soaking, pot experiment; The result shows: the metabolite of bacterial strain SF-1 is to the growth nonhazardous effect of milpa; Can promote simultaneously corn seed sprouting, growing of plumule and radicle also had certain facilitation; Milpa through the metabolite of bacterial strain SF-1 was handled is compared with untreated milpa, and the former helps the growth of milpa under ground portion, increases fibrous root, improves root/shoot ratio; Promote root growth, promote growing of whole strain plant; What said milpa was selected for use is that land-reclaimable academy of sciences corn breeding research department, Heilongjiang Province cultivates silk dust-brand susceptible kind T-4019.
The physicochemical character of the SF-1 water-soluble biological seed coating agent of control of maize head smut is measured the result in this embodiment: viscosity is 431.00mPa.S, and the pH value is 6.5; Film formation time is 12h; Coating uniformity is good, and the dressing expulsion rate is 3.25%, has good stability.
The metabolite of bacterial strain SF-1 in this embodiment, to the mensuration of teliospore germination inhibiting rate: with the metabolite of bacterial strain SF-1, the mother liquor of drawing 1mL respectively with pipette adds in the scale test tube of sterilization constant volume to 5mL; Biological seed coating agent after the dilution is joined mixing in the accurate quantitative 25mL water maltose agar medium of thawing; Pour in the sterilization culture dish; Process the band medicine medium of variable concentrations, the mass concentration of band medicine medium is all calculated by the effective content of bactericide in the biological seed coating agent; Getting the 400 μ m spore suspensions that configure is put in band medicine medium central authorities and smoothens; 28 ℃ of constant temperature culture 48h; Teliospore germination surpasses the teleutospore radius with promycelium length and is as the criterion; The result is: the teliospore germination inhibiting rate is 76.56%.
Embodiment two: this embodiment and embodiment one are different be the SF-1 water-soluble biological seed coating agent of control of maize head smut be every 100mL by volume portion rate form by the distilled water of the metabolite of 18 parts of bacterial strain SF-1,2 parts of agricultural enzyme microorganisms (BYM), 0.02 part of zinc sulphate, 0.01 part of boric acid, 2.5 parts of polyoxyethylene, 2 parts of polyethylene glycol, 1 part of water-soluble Yihong, 4 parts of NaCl, 5 parts of ethylene glycol and surplus.Other is identical with embodiment one.
Embodiment three: this embodiment and embodiment one are different be the SF-1 water-soluble biological seed coating agent of control of maize head smut be every 100mL by volume portion rate form by the distilled water of the metabolite of 22 parts of bacterial strain SF-1,5 parts of agricultural enzyme microorganisms (BYM), 0.04 part of zinc sulphate, 0.02 part of boric acid, 2.5 parts of polyoxyethylene, 2 parts of polyethylene glycol, 2 parts of water-soluble Yihong, 4 parts of NaCl, 5 parts of ethylene glycol and surplus.Other is identical with embodiment one.
Embodiment four: this embodiment and embodiment one are different be the SF-1 water-soluble biological seed coating agent of control of maize head smut be every 100mL by volume portion rate form by the distilled water of the metabolite of 19～21 parts of bacterial strain SF-1,3～4 parts of agricultural enzyme microorganisms (BYM), 0.025～0.035 part of zinc sulphate, 0.012～0.018 part of boric acid, 2.5 parts of polyoxyethylene, 2 parts of polyethylene glycol, 1.2～1.8 parts of water-soluble Yihong, 4 parts of NaCl, 5 parts of ethylene glycol and surplus.Other is identical with embodiment one.
Embodiment five: this embodiment and embodiment one are different be the SF-1 water-soluble biological seed coating agent of control of maize head smut be every 100mL by volume portion rate form by the distilled water of the metabolite of 20 parts of bacterial strain SF-1,3 parts of agricultural enzyme microorganisms (BYM), 0.03 part of zinc sulphate, 0.015 part of boric acid, 2.5 parts of polyoxyethylene, 2 parts of polyethylene glycol, 1.5 parts of water-soluble Yihong, 4 parts of NaCl, 5 parts of ethylene glycol and surplus.Other is identical with embodiment one.
Embodiment six: this embodiment and embodiment one are different be the SF-1 water-soluble biological seed coating agent of control of maize head smut be every 100mL by volume portion rate form by the distilled water of the metabolite of 21 parts of bacterial strain SF-1,4 parts of agricultural enzyme microorganisms (BYM), 0.02 part of zinc sulphate, 0.02 part of boric acid, 2.5 parts of polyoxyethylene, 2 parts of polyethylene glycol, 1.8 parts of water-soluble Yihong, 4 parts of NaCl, 5 parts of ethylene glycol and surplus.Other is identical with embodiment one.
Embodiment seven: what this embodiment and embodiment one to six were different is the preparation of the metabolite of bacterial strain SF-1: one, the cultured SF-1 of purifying is processed the bacterium dish; Bacterium dish with 1.6ml is inoculated in the fermentation culture of 80ml then; In temperature is to cultivate 96h, centrifugal again 15min under the condition that 32 ℃, pH value are 7, rotating speed is 180r/min; Two, get supernatant after centrifugal and filter, obtain the metabolite of bacterial strain SF-1 with biofilter; Wherein fermentation culture is that every 100mL fermentation culture is by the big beans filtrating of 1.5g, 0.3g peptone, 0.25g sulfate of ammoniac, 2.0g glucose, 1g starch in the step 1; 0.025g the distilled water of magnesium sulfate, 0.02g potassium dihydrogen phosphate, 0.2g sodium chloride, 0.2g calcium carbonate and surplus is formed; PH value is 7～8, moist heat sterilization 30min; Said big beans filtrating is filtered behind water boil 0.5～1h for big beans adds and is obtained.Other and embodiment one to six phase are together.
Embodiment eight: the SF-1 water-soluble biological seed coating agent of this embodiment control of maize head smut SF-1 water-soluble biological seed coating agent of portion rate control of maize head smut by volume be every 100mL by volume portion rate form by the distilled water of the metabolite of 20 parts of bacterial strain SF-1,3 parts of agricultural enzyme microorganisms (BYM), 0.03 part of zinc sulphate, 0.015 part of boric acid, 2.5 parts of polyoxyethylene, 2 parts of polyethylene glycol, 1.5 parts of water-soluble Yihong, 4 parts of NaCl, 5 parts of ethylene glycol and surplus; Wherein bacterial strain SF-1 is Jia Dena streptomycete Streptomyces gardneri.
The SF-1 water-soluble biological seed coating agent of control of maize head smut in this embodiment, carry out field control effectiveness test:
Biological seed coating agent and corn seed are carried out the wet-mixing kind, and stirring is placed on aeration-drying place, can sow after the drying; Dressing is not a blank, is the medicament contrast with 2% Garrick show;
Arrange sub-district district's group at random, 3 repetitions, sub-district area 20m
2, the long 5m of row, 6 row districts, line-spacing 0.68m, other field management measures are normal;
To the emergence of corn and the influence of growing, the result is as shown in table 1, and difference is compared on 1% level in the influence of emergence of corn rate with other two groups not remarkable for biological seed coating agent in this embodiment; And observe in whole growing; Compare at each equal indifference of stage breeding time of later stage with other two groups; Shooting stage, tasseling stage, plant height, leaf look, fruit ear shape and maturing stage are all consistent, and this SF-1 water-soluble biological seed coating agent that shows control of maize head smut in this embodiment is to emergence of corn and grow identical with the effect of existing chemical agent (2% Garrick show).
Table 1
The SF-1 water-soluble biological seed coating agent of control of maize head smut reaches 86.5% to the control efficiency of maize head smut in this embodiment, is more or less the same with the control efficiency 86.72% of existing chemical agent (2% Garrick show), can the instead of chemical medicament.
The SF-1 water-soluble biological seed coating agent of control of maize head smut is to the influence of corn yield in this embodiment, and the result is as shown in table 2, and biological seed coating agent has played facilitation to the raising the output of corn, and rate of growth reaches 13.69%.
Table 2
Embodiment nine: the SF-1 water-soluble biological seed coating agent of this embodiment control of maize head smut be every 100mL by volume portion rate form by the distilled water of the metabolite of 21 parts of bacterial strain SF-1,4 parts of agricultural enzyme microorganisms (BYM), 0.02 part of zinc sulphate, 0.02 part of boric acid, 2.5 parts of polyoxyethylene, 2 parts of polyethylene glycol, 1.8 parts of water-soluble Yihong, 4 parts of NaCl, 5 parts of ethylene glycol and surplus; Wherein bacterial strain SF-1 is Jia Dena streptomycete Streptomyces gardneri.
The SF-1 water-soluble biological seed coating agent of control of maize head smut reaches 86.82% to the control efficiency of maize head smut in this embodiment, is more or less the same with the control efficiency 86.72% of existing chemical agent (2% Garrick show), can the instead of chemical medicament.
The SF-1 water-soluble biological seed coating agent of control of maize head smut has been played facilitation to the raising the output of corn in this embodiment, and rate of growth reaches 14.28%.
Claims (3)
1. the SF-1 water-soluble biological seed coating agent of control of maize head smut, the SF-1 water-soluble biological seed coating agent that it is characterized in that the control of maize head smut be every 100mL by volume portion rate form by the distilled water of the metabolite of 20 parts of bacterial strain SF-1,2～5 parts of agricultural enzyme microorganisms, 0.02～0.04 part of zinc sulphate, 0.01～0.02 part of boric acid, 2.5 parts of polyoxyethylene, 2 parts of polyethylene glycol, 1～2 part of water-soluble Yihong, 4 parts of NaCl, 5 parts of ethylene glycol and surplus; Wherein bacterial strain SF-1 be the Jia Dena streptomycete (
Streptomyces gardneri), its deposit number is CGMCC3928.
2. the SF-1 water-soluble biological seed coating agent of control of maize head smut according to claim 1, the SF-1 water-soluble biological seed coating agent that it is characterized in that the control of maize head smut be every 100mL by volume portion rate form by the distilled water of the metabolite of 20 parts of bacterial strain SF-1,3 parts of agricultural enzyme microorganisms, 0.03 part of zinc sulphate, 0.015 part of boric acid, 2.5 parts of polyoxyethylene, 2 parts of polyethylene glycol, 1.5 parts of water-soluble Yihong, 4 parts of NaCl, 5 parts of ethylene glycol and surplus.
3. the SF-1 water-soluble biological seed coating agent of control of maize head smut according to claim 1 and 2; It is characterized in that the preparation of 1 metabolite of bacterial strain SF-: one, the cultured SF-1 of purifying is processed the bacterium dish; Bacterium dish with 1.6ml is inoculated in the fermentation culture of 80ml then; In temperature is to cultivate 96h, centrifugal again 15min under the condition that 32 ℃, pH value are 7, rotating speed is 180r/min; Two, get supernatant after centrifugal and filter, obtain 1 metabolite of bacterial strain SF-with biofilter; Wherein fermentation culture is that every 100mL fermentation culture is by the big beans filtrating of 1.5g, 0.3g peptone, 0.25g sulfate of ammoniac, 2.0g glucose, 1g starch in the step 1; 0.025g the distilled water of magnesium sulfate, 0.02g potassium dihydrogen phosphate, 0.2g sodium chloride, 0.2g calcium carbonate and surplus is formed; PH value is 7～8, moist heat sterilization 30 min; Said big beans filtrating is filtered behind water boil 0.5～1h for big beans adds and is obtained.
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| CN2010102365365A CN101878782B (en) | 2010-07-26 | 2010-07-26 | SF-1 water-soluble biological seed coating for preventing and curing maize head smut |
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| CN2010102365365A CN101878782B (en) | 2010-07-26 | 2010-07-26 | SF-1 water-soluble biological seed coating for preventing and curing maize head smut |
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| CN1023758C (en) * | 1986-12-15 | 1994-02-16 | 伊莱利利公司 | Antibiotic A 10255 complex and factors, process, microorganisms, and production therefor |
| WO2008143146A2 (en) * | 2007-05-11 | 2008-11-27 | Sumitomo Chemical Company, Limited | Method for producing glyceric acid |
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2010
- 2010-07-26 CN CN2010102365365A patent/CN101878782B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5229362A (en) * | 1986-12-15 | 1993-07-20 | Eli Lilly And Company | Antibiotic A10255 complex and factors, and process and production therefor |
| CN1023758C (en) * | 1986-12-15 | 1994-02-16 | 伊莱利利公司 | Antibiotic A 10255 complex and factors, process, microorganisms, and production therefor |
| WO2008143146A2 (en) * | 2007-05-11 | 2008-11-27 | Sumitomo Chemical Company, Limited | Method for producing glyceric acid |
Non-Patent Citations (2)
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| Favret Montgomery E.等.Effect of cobalt and cyanocobalamin on biosynthesis of A10255 |
| Favret, Montgomery E.等.Effect of cobalt and cyanocobalamin on biosynthesis of A10255, a thiopeptide antibiotic complex.《Journal of Antibiotics》.1992,第45卷(第11期),1809-11. * |
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