CN101875647A - Method for improving plant antibiotic chalcomoracin content in mulberry leaves - Google Patents
Method for improving plant antibiotic chalcomoracin content in mulberry leaves Download PDFInfo
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- CN101875647A CN101875647A CN2010101863293A CN201010186329A CN101875647A CN 101875647 A CN101875647 A CN 101875647A CN 2010101863293 A CN2010101863293 A CN 2010101863293A CN 201010186329 A CN201010186329 A CN 201010186329A CN 101875647 A CN101875647 A CN 101875647A
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- mulberry leaves
- mulberry leaf
- chalcomoracin
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- plant antibiotic
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Abstract
The invention relates to a method for improving plant antibiotic chalcomoracin content in mulberry leaves. The plant antibiotic chalcomoracin is a Diels-Alder adduct, and has narrow distribution in the mulberry leaves. The method comprises the following steps of: washing freshly picked mulberry leaves, and irradiating the mulberry leaves by ultraviolet light with one or any combination of a UVA band, a UVB band or a UVC band; controlling the irradiation intensity of the ultraviolet light to be between 10 and 1,000 W, and controlling the irradiation time of the ultraviolet light to be 0.5 to 48 hours; maintaining air humidity to be between 10 and 100 percent during the irradiation; and drying the irradiated mulberry leaves in the shade or drying the irradiated mulberry leaves, and controlling drying temperature to be less than or equal to 90 DEG C. The method can improve the plant antibiotic chalcomoracin content in the mulberry leaves remarkably, or can induce the mulberry leaves to generate a novel Diels-Alder adduct to enhance the antibiotic activity of the induced mulberry leaves, and makes the mulberry leaves become a material from which the plant antibiotic is extracted.
Description
Technical field
The invention belongs to biological pharmacy technical field, relate to a kind of method that improves plant antibiotic and other Diels-Aldel type adducts content in the mulberry leaf of inducing.
Background technology
Mulberry leaf are the dry leave of moraceae plants mulberry, contain Inokosterone, ecdysterone, β-Bao sterol, phytomelin, mulberry glucoside, trifoliin, Umbelliferone, Scopolin, trigonelline, choline, VITAMIN B4, aspartic acid, chlorogenic acid etc.Mulberry leaf have effects such as spasmolysis, anti-inflammatory, hypoglycemic, hypertension.
The plant antibiotic of indication of the present invention is a kind of Diels-Alder type adducts, and representation compound is chalcomoracin, and structural formula is:
Related Experimental Study proves that this compound has the anti-microbial activity of highly significant, is a kind of plant antibiotic.Diels-Alder type adducts is in natural narrow distribution range, only is distributed in the middle of the root skin of mulberry leaf with trace.By the technology of the present invention, can significantly improve the content of this constituents in the mulberry leaf, maybe can induce mulberry leaf to produce new Diels-Alder type adducts, the mulberry leaf anti-microbial activity after inducing is strengthened, become the raw material that extracts such plant antibiotic.
Summary of the invention
The purpose of this invention is to provide a kind of method that improves plant antibiotic chalcomoracin content in the mulberry leaf.By this method, can significantly improve the content of mulberry leaf plant antibiotic chalcomoracin and its prerequisite thing and other Diels-Alder type adducts, the raising rate is not less than 10%.
The mulberry leaf of fresh harvesting are cleaned the back and are shone under UV-light, and described UV-light is one or more combinations in UVA wave band, UVB wave band or the UVC wave band; UV-irradiation intensity is 10～1000w, and the UV-irradiation time is 0.5～48 hour; Keep atmospheric moisture 10～100% in the irradiation process; Postradiation mulberry leaf dry in the shade or dry, and bake out temperature is smaller or equal to 90 ℃.
Described UV-light is preferably one or both the combination in UVA wave band or the UVB wave band;
Described UV-irradiation intensity is preferably 20～100w;
The described UV-irradiation time is preferably 1～6 hour;
Keep atmospheric moisture to be preferably 50～95% in the described irradiation process.
The inventive method can significantly improve the content of plant antibiotic chalcomoracin in the mulberry leaf, the raising rate is not less than 10%, maybe can induce mulberry leaf to produce new Diels-Alder type adducts, the mulberry leaf anti-microbial activity after inducing be strengthened, and can be become the raw material that extracts such plant antibiotic.
Embodiment
Embodiment 1
Fresh mulberry leaf are cleaned under the UVA UV-light that the back is 10w in intensity and were shone 48 hours, and keeping atmospheric moisture in the irradiation process is 10%.Postradiation mulberry leaf dry in the shade.Mulberry leaf after drying in the shade are analyzed through HPLC, compare with the mulberry leaf that do not have irradiation, and the content of plant antibiotic chalcomoracin has increased by 17.5%.
Embodiment 2
Fresh mulberry leaf are cleaned under the UVB UV-light that the back is 1000w in intensity and were shone 0.5 hour, and keeping atmospheric moisture in the irradiation process is 100%.60 ℃ of oven dry of postradiation mulberry leaf.Mulberry leaf after the oven dry are analyzed through HPLC, compare with the mulberry leaf that do not have irradiation, and the content of plant antibiotic chalcomoracin has increased by 25.5%.
Embodiment 3
Fresh mulberry leaf are cleaned 6 hours mulberry leaf of irradiation under the UVC UV-light that the back is 100w in intensity, and keeping atmospheric moisture in the irradiation process is 30%.20 ℃ of oven dry of postradiation mulberry leaf.After the oven dry mulberry leaf analyze through HPLC, with do not have the irradiation mulberry leaf compare, the content of plant antibiotic chalcomoracin has increased by 45.3%.
Embodiment 4
It is to shine simultaneously 24 hours under the UVA UV-light of 20w and the UVB UV-light that intensity is 30w in intensity that fresh mulberry leaf are cleaned the back, and keeping atmospheric moisture in the irradiation process is 50%.50 ℃ of oven dry of postradiation mulberry leaf.After the oven dry mulberry leaf analyze through HPLC, with do not have the irradiation mulberry leaf compare, the content of plant antibiotic chalcomoracin has increased by 90%.
Embodiment 5
It is to shine simultaneously 12 hours under the UVA UV-light of 30w and the UVC UV-light that intensity is 50w in intensity that fresh mulberry leaf are cleaned the back, and keeping atmospheric moisture in the irradiation process is 70%.30 ℃ of oven dry of postradiation mulberry leaf.After the oven dry mulberry leaf analyze through HPLC, with do not have the irradiation mulberry leaf compare, the content of plant antibiotic chalcomoracin has increased by 61.5%.
Embodiment 6
It is to shine simultaneously 3 hours under the UVB UV-light of 200w and the UVC UV-light that intensity is 150w in intensity that fresh mulberry leaf are cleaned the back, and keeping atmospheric moisture in the irradiation process is 90%.80 ℃ of oven dry of postradiation mulberry leaf.After the oven dry mulberry leaf analyze through HPLC, with do not have the irradiation mulberry leaf compare, the content of plant antibiotic chalcomoracin has increased by 45.3%.
Embodiment 7
Fresh mulberry leaf clean the back intensity be the UVA UV-light of 500w, UVB UV-light that intensity is 500w and intensity be 500w the UVC UV-light under shone simultaneously 1 hour, keeping atmospheric moisture in the irradiation process is 95%.90 ℃ of oven dry of postradiation mulberry leaf.After the oven dry mulberry leaf analyze through HPLC, with do not have the irradiation mulberry leaf compare, the content of plant antibiotic chalcomoracin has increased by 27.6%.
Claims (5)
1. method that improves plant antibiotic chalcomoracin content in the mulberry leaf is characterized in that: the mulberry leaf of fresh harvesting are cleaned the back and are shone under UV-light, and described UV-light is one or more combinations in UVA wave band, UVB wave band or the UVC wave band; UV-irradiation intensity is 10～1000w, and the UV-irradiation time is 0.5～48 hour; Keep atmospheric moisture 10～100% in the irradiation process; Postradiation mulberry leaf dry in the shade or dry, and bake out temperature is smaller or equal to 90 ℃.
2. a kind of method that improves plant antibiotic chalcomoracin content in the mulberry leaf according to claim 1 is characterized in that: described UV-light is one or both the combination in UVA wave band or the UVB wave band.
3. a kind of method that improves plant antibiotic chalcomoracin content in the mulberry leaf according to claim 1, it is characterized in that: described UV-irradiation intensity is 20～100w.
4. a kind of method that improves plant antibiotic chalcomoracin content in the mulberry leaf according to claim 1, it is characterized in that: the described UV-irradiation time is 1～6 hour.
5. a kind of method that improves plant antibiotic chalcomoracin content in the mulberry leaf according to claim 1 is characterized in that: keep atmospheric moisture 50～95% in the described irradiation process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101863293A CN101875647A (en) | 2010-05-31 | 2010-05-31 | Method for improving plant antibiotic chalcomoracin content in mulberry leaves |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101863293A CN101875647A (en) | 2010-05-31 | 2010-05-31 | Method for improving plant antibiotic chalcomoracin content in mulberry leaves |
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| CN101875647A true CN101875647A (en) | 2010-11-03 |
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| CN2010101863293A Pending CN101875647A (en) | 2010-05-31 | 2010-05-31 | Method for improving plant antibiotic chalcomoracin content in mulberry leaves |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107114561A (en) * | 2017-04-18 | 2017-09-01 | 浙江大学 | A kind of induction mulberry leaf for preventing and treating silkworm BmNPV disease and application thereof |
| CN108034641A (en) * | 2018-01-24 | 2018-05-15 | 浙江大学 | Isolation and purification method of Diels-Alderase enzymes and application thereof in a kind of uv induction mulberry leaf |
| CN108250167A (en) * | 2018-01-16 | 2018-07-06 | 浙江大学 | Biologically active monomer ingredient chalcomoracin and its preparation method and application in mulberry leaf based on uv induction |
-
2010
- 2010-05-31 CN CN2010101863293A patent/CN101875647A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 《2009年中国药学大会暨第九届中国药师周论文集》 20091121 谷希达等 基于光酶诱导药材中次生代谢产物变化的研究 3651-3661 , 2 * |
| 《Journal of Insect Biotechnology and Sericology》 20031231 Rashmi Sharma et al Enhanced Induction of Proline and Moracins in UV Stressed Mulberry Plants 127-131 第72卷, 2 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107114561A (en) * | 2017-04-18 | 2017-09-01 | 浙江大学 | A kind of induction mulberry leaf for preventing and treating silkworm BmNPV disease and application thereof |
| CN108250167A (en) * | 2018-01-16 | 2018-07-06 | 浙江大学 | Biologically active monomer ingredient chalcomoracin and its preparation method and application in mulberry leaf based on uv induction |
| CN108034641A (en) * | 2018-01-24 | 2018-05-15 | 浙江大学 | Isolation and purification method of Diels-Alderase enzymes and application thereof in a kind of uv induction mulberry leaf |
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Open date: 20101103 |