CN101874904A - Method for preparing medicament-carrying tissue engineering hydrogel - Google Patents
Method for preparing medicament-carrying tissue engineering hydrogel Download PDFInfo
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- CN101874904A CN101874904A CN2009102497643A CN200910249764A CN101874904A CN 101874904 A CN101874904 A CN 101874904A CN 2009102497643 A CN2009102497643 A CN 2009102497643A CN 200910249764 A CN200910249764 A CN 200910249764A CN 101874904 A CN101874904 A CN 101874904A
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Abstract
The invention discloses a method for preparing medicament-carrying tissue engineering hydrogel, which comprises the following steps: a, dissolving collagen in 0.50mol/L solution of acetone to prepare solution of collagen; b, dissolving hyaluronic acid in 0.50 mol/L solution of acetone to prepare solution of hyaluronic acid; c, dissolving sodium alga acid in 0.50 mol/L solution of acetone to prepare solution of sodium alga acid; d, mixing the solution of collagen, the solution of hyaluronic acid and the solution of sodium alga acid in a weight part ratio of 4:3:3; e, adding 0.1mg/ml medicament into the mixed solution; f, adding glutaraldehyde into the mixed solution to perform cross linking to obtain the medicament-carrying tissue engineering hydrogel; and g, disinfecting the medicament-carrying tissue engineering hydrogel by using Co60, and packing the medicament-carrying tissue engineering hydrogel for later use.
Description
Technical field
The present invention relates to a kind of preparation method that can be used as the medicament-carrying tissue engineering hydrogel of medical material.
Background technology
Collagen is a kind of common hydrogel material, is fit to the reparation and the regeneration of human organ, can promote the growth of wound healing and granulation tissue, has good hemostasis and packing action, can be used for promoting wound healing, hemostasis, the filling of operation residual cavity etc.Hyaluronic acid is the important composition thing of glycosaminoglycans in the n cell epimatrix, and it plays an important role in wound healing, and hyaluronic acid can be by the hyaluronic acid enzymatic degradation in cell and the serum, and hyaluronic mechanical strength is lower.Alginic acid is the polysaccharide that is extracted by Brown algae, is commonly used for hemostatic material and wound dressing.Therefore, in these hydrogel materials, add a certain amount of medicine, can when having good biocompatibility and hemostasis filling performance, play the effect of medicament slow release.
By retrieval, do not see the report that uses this preparation method and material thereof before this.
Summary of the invention
The invention provides a kind of preparation method that can be used as the medicament-carrying tissue engineering hydrogel of medical material.
The following technical scheme of the concrete employing of the present invention:
A kind of preparation method of medicament-carrying tissue engineering hydrogel comprises the following steps:
A. collagen protein is dissolved in the acetone soln that concentration is 0.5mol/L and makes collagen solution; B. hyaluronic acid is dissolved in the acetone soln that concentration is 0.5mol/L and makes hyaluronic acid solution; C is dissolved in sodium alginate in the acetone soln that concentration is 0.5mol/L and makes hyaluronic acid solution; .d. with collagen solution, hyaluronic acid solution and sodium alginate soln be 4: 3: 3 mixed of mark by weight; E. adding concentration in mixed solution is the medicine of 0.1mg/ml; F. will in mixed solution, add glutaraldehyde and make its crosslinked medicament-carrying tissue engineering hydrogel that obtains; G. use the Co60 sterilization, package spare.
In the preparation method of above-mentioned porous collagen/polylactic acid bracket, epithelical cell growth factor EGF is 0.05mg/ml with respect to the concentration of mixed solution.
The preparation method of above-mentioned medicament-carrying tissue engineering hydrogel, medicine can be selected hemostasis or anti-inflammation drugs.
Beneficial effect of the present invention is: the invention provides a kind of preparation method that has good biocompatibility and can carry out the medicament-carrying tissue engineering hydrogel of medicament slow release.
The specific embodiment
Embodiment 1:
A kind of preparation method of medicament-carrying tissue engineering hydrogel comprises the following steps: that a. is dissolved in collagen protein in the acetone soln that concentration is 0.5mol/L to make collagen solution; B. hyaluronic acid is dissolved in the acetone soln that concentration is 0.5mol/L and makes hyaluronic acid solution; C is dissolved in sodium alginate in the acetone soln that concentration is 0.5mol/L and makes hyaluronic acid solution; .d. with collagen solution, hyaluronic acid solution and sodium alginate soln be 4: 3: 3 mixed of mark by weight; E. adding concentration in mixed solution is the aspirin of 0.1mg/ml; F. will in mixed solution, add the 10ml glutaraldehyde and make its crosslinked medicament-carrying tissue engineering hydrogel that obtains; G. use the Co60 sterilization, package spare.
Embodiment 2:
A kind of preparation method of medicament-carrying tissue engineering hydrogel comprises the following steps: that a. is dissolved in collagen protein in the acetone soln that concentration is 0.5mol/L to make collagen solution; B. hyaluronic acid is dissolved in the acetone soln that concentration is 0.5mol/L and makes hyaluronic acid solution; C is dissolved in sodium alginate in the acetone soln that concentration is 0.5mol/L and makes hyaluronic acid solution; .d. with collagen solution, hyaluronic acid solution and sodium alginate soln be 4: 3: 3 mixed of mark by weight; E. in mixed solution, add the 10g rutosids; F. will in mixed solution, add the 10ml glutaraldehyde and make its crosslinked medicament-carrying tissue engineering hydrogel that obtains; G. use the Co60 sterilization, package spare.
Claims (3)
1. the preparation method of a medicament-carrying tissue engineering hydrogel comprises the following steps: that a. is dissolved in collagen protein in the acetone soln that concentration is 0.5mol/L to make collagen solution; B. hyaluronic acid is dissolved in the acetone soln that concentration is 0.5mol/L and makes hyaluronic acid solution; C is dissolved in sodium alginate in the acetone soln that concentration is 0.5mol/L and makes hyaluronic acid solution; .d. with collagen solution, hyaluronic acid solution and sodium alginate soln be 4: 3: 3 mixed of mark by weight; E. adding concentration in mixed solution is the medicine of 0.1mg/ml; F. will in mixed solution, add glutaraldehyde and make its crosslinked medicament-carrying tissue engineering hydrogel that obtains; G. use the Co60 sterilization, package spare.
2. the preparation method of medicament-carrying tissue engineering hydrogel according to claim 1 is characterized in that described medicine can be haemostatic medicament.
3. the preparation method of medicament-carrying tissue engineering hydrogel according to claim 1 is characterized in that described medicine can be anti-inflammation drugs.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102497643A CN101874904A (en) | 2009-12-02 | 2009-12-02 | Method for preparing medicament-carrying tissue engineering hydrogel |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009102497643A CN101874904A (en) | 2009-12-02 | 2009-12-02 | Method for preparing medicament-carrying tissue engineering hydrogel |
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| CN101874904A true CN101874904A (en) | 2010-11-03 |
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| CN2009102497643A Pending CN101874904A (en) | 2009-12-02 | 2009-12-02 | Method for preparing medicament-carrying tissue engineering hydrogel |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102070786B (en) * | 2009-11-19 | 2012-05-30 | 中国农业科学院农产品加工研究所 | Hyaluronic acid-sodium alginate composite hydrogel and preparation method thereof |
| CN102657871A (en) * | 2012-05-04 | 2012-09-12 | 中国药科大学 | Oral slow release preparation, entrapment material and preparation method |
| CN104327311A (en) * | 2014-10-27 | 2015-02-04 | 天津科技大学 | Hyaluronic acid composite cross-linked hydrogel and preparation method thereof |
| CN107325300A (en) * | 2017-06-21 | 2017-11-07 | 深圳市第二人民医院 | A kind of pH sensitive aquagels and its preparation and application |
| CN109535447A (en) * | 2018-11-06 | 2019-03-29 | 福建农林大学 | A kind of thermal sensitivity collagen nanofiber/PNIPAM semi-intercrossing network formula hydrogel and preparation method thereof |
-
2009
- 2009-12-02 CN CN2009102497643A patent/CN101874904A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102070786B (en) * | 2009-11-19 | 2012-05-30 | 中国农业科学院农产品加工研究所 | Hyaluronic acid-sodium alginate composite hydrogel and preparation method thereof |
| CN102657871A (en) * | 2012-05-04 | 2012-09-12 | 中国药科大学 | Oral slow release preparation, entrapment material and preparation method |
| CN102657871B (en) * | 2012-05-04 | 2014-01-01 | 中国药科大学 | Oral slow release preparation, entrapment material and preparation method |
| CN104327311A (en) * | 2014-10-27 | 2015-02-04 | 天津科技大学 | Hyaluronic acid composite cross-linked hydrogel and preparation method thereof |
| CN104327311B (en) * | 2014-10-27 | 2017-06-06 | 天津科技大学 | A kind of hyaluronic acid composite crosslinking hydrogel and preparation method thereof |
| CN107325300A (en) * | 2017-06-21 | 2017-11-07 | 深圳市第二人民医院 | A kind of pH sensitive aquagels and its preparation and application |
| CN107325300B (en) * | 2017-06-21 | 2019-12-27 | 深圳市第二人民医院 | pH sensitive hydrogel and preparation and application thereof |
| CN109535447A (en) * | 2018-11-06 | 2019-03-29 | 福建农林大学 | A kind of thermal sensitivity collagen nanofiber/PNIPAM semi-intercrossing network formula hydrogel and preparation method thereof |
| CN109535447B (en) * | 2018-11-06 | 2021-08-24 | 福建农林大学 | Thermosensitive collagen nanofiber/PNIPAM semi-interpenetrating network type hydrogel and preparation method thereof |
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Application publication date: 20101103 |