CN101703806A - Porous hydroxyapatite/chitosan-gelatine composite material bracket - Google Patents
Porous hydroxyapatite/chitosan-gelatine composite material bracket Download PDFInfo
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- CN101703806A CN101703806A CN200910250313A CN200910250313A CN101703806A CN 101703806 A CN101703806 A CN 101703806A CN 200910250313 A CN200910250313 A CN 200910250313A CN 200910250313 A CN200910250313 A CN 200910250313A CN 101703806 A CN101703806 A CN 101703806A
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- chitosan
- gelatine
- composite material
- hydroxyapatite
- porous hydroxyapatite
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Abstract
The invention discloses a porous hydroxyapatite/chitosan-gelatine composite material bracket, comprising the following components of hydroxyapatite, chitosan and gelatine according to weight ratio of 3:3:4. The porous hydroxyapatite/chitosan-gelatine composite material bracket can be prepared by the following steps: a. preparing 2 to 20mum of HA powder into water suspension; b. adding water solution of the chitosan and the gelatine in the water suspension; c. adding 10ml of glutaraldehyde to prepare HA-chitosan-gelatine net composition in a cross-linking manner; d. prefreezing the composition at the temperature of minus 40 DEG C and then preparing a porous bracket; and e. using sodium hydroxide to neutralize the rest of acetic acid, using NaBH4 solution to treat and eliminate the residual glutaraldehyde, and carrying out secondary freeze-drying, thus obtaining the porous hydroxyapatite/chitosan-gelatine composite material bracket with the porosity of more than 90 percent and the pore diameter of 300 to 400mum.
Description
Technical field
The present invention relates to a kind of porous hydroxyapatite/chitosan sugar-gelatine composite material bracket.
Background technology
Collagen is a kind of common hydrogel material, is fit to the reparation and the regeneration of human organ, can promote the growth of wound healing and granulation tissue, has good hemostasis and packing action, can be used for promoting wound healing, hemostasis, the filling of operation residual cavity etc.Gelatin is that collagen partly is out of shape derivant, and it is disintegrated by the triple helices structure of collagen is that single chain molecule forms.Chitosan is a kind of alkaline polysaccharide of uniqueness, is prepared by de-acetyl chitin, has excellent biological compatibility, is a kind of common bio-medical material.Hydroxyapatite is a kind of common bone tissue engineer inorganic material, has excellent biological compatibility and mechanical property.Therefore, these three kinds of materials are combined with each other, can integrate the advantage of different materials, obtain a kind of new porous hydroxyapatite/chitosan sugar-gelatine composite material bracket.
By retrieval, do not see the report that uses this preparation method and material thereof before this.
Summary of the invention
The invention provides a kind of porous hydroxyapatite/chitosan sugar-gelatine composite material bracket.
The following technical scheme of the concrete employing of the present invention:
A kind of porous hydroxyapatite/chitosan sugar-gelatine composite material bracket, it is made of by weight 3: 3: 4 hydroxyapatite, chitosan and gelatin, can be prepared from by following steps:: a. makes water slurry with the HA powder body of 2-20 μ m; B. in water slurry, add the aqueous solution and the gelatin of chitosan; C. add the 10ml glutaraldehyde cross-linking and prepare HA-chitosan-gelatin network constituent; D. this constituent is made porous support after-40 ℃ of pre-freezes; E. use in the sodium hydroxide solution and residual acetic acid, eliminate residual glutaraldehyde, obtain porosity greater than 90% through the secondary lyophilizing, porous hydroxyapatite/chitosan sugar-gelatin support of aperture 300-400 μ m with the NaBH4 solution-treated.
Above-mentioned porous hydroxyapatite/chitosan sugar-gelatine composite material bracket, the concentration that it is characterized in that sodium hydroxide solution described in the preparation process is 0.5mol/l.
Beneficial effect of the present invention is: the invention provides and a kind ofly have good biocompatibility and mechanical property, and have the porous hydroxyapatite/chitosan sugar-gelatine composite material bracket of anthemorrhagic performance.
The specific embodiment
Embodiment 1:
A kind of porous hydroxyapatite/chitosan sugar-gelatine composite material bracket, its preparation method comprise the following steps: that a. makes water slurry with the HA powder body of 2-20 μ m; B. in water slurry, add the aqueous solution and the gelatin of chitosan; C. add the 10ml glutaraldehyde cross-linking and prepare HA-chitosan-gelatin network constituent; D. this constituent is made porous support after-40 ℃ of pre-freezes; E. use in the sodium hydroxide solution of 0.05mol/L and residual acetic acid, eliminate residual glutaraldehyde with the NaBH4 solution-treated, obtaining porosity through the secondary lyophilizing is 95%, porous hydroxyapatite/chitosan sugar-gelatin support of aperture 300-400 μ m.
Embodiment 2:
A kind of porous hydroxyapatite/chitosan sugar-gelatine composite material bracket, its preparation method comprise the following steps: that a. makes water slurry with the HA powder body of 2-20 μ m; B. in water slurry, add the acetone soln and the gelatin of chitosan; C. add the 15ml glutaraldehyde cross-linking and prepare HA-chitosan-gelatin network constituent; D. this constituent is made porous support after-40 ℃ of pre-freezes; E. use in the 0.1mol/L sodium hydroxide solution and residual acetic acid, eliminate residual glutaraldehyde with the NaBH4 solution-treated, obtaining porosity through the secondary lyophilizing is 90%, porous hydroxyapatite/chitosan sugar-gelatin support of aperture 300-400 μ m.
Claims (2)
1. porous hydroxyapatite/chitosan sugar-gelatine composite material bracket, it is characterized in that it is made of by weight 3: 3: 4 hydroxyapatite, chitosan and gelatin, can be prepared from by following steps:: a. makes water slurry with the HA powder body of 2-20 μ m; B. in water slurry, add the aqueous solution and the gelatin of chitosan; C. add the 10ml glutaraldehyde cross-linking and prepare HA-chitosan-gelatin network constituent; D. this constituent is made porous support after-40 ℃ of pre-freezes; E. use in the sodium hydroxide solution and residual acetic acid, eliminate residual glutaraldehyde, obtain porosity greater than 90% through the secondary lyophilizing, porous hydroxyapatite/chitosan sugar-gelatin support of aperture 300-400 μ m with the NaBH4 solution-treated.
2. porous hydroxyapatite/chitosan sugar-gelatine composite material bracket according to claim 1, the concentration that it is characterized in that the employed sodium hydroxide solution of preparation process is 0.5mol/l.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910250313A CN101703806A (en) | 2009-12-03 | 2009-12-03 | Porous hydroxyapatite/chitosan-gelatine composite material bracket |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910250313A CN101703806A (en) | 2009-12-03 | 2009-12-03 | Porous hydroxyapatite/chitosan-gelatine composite material bracket |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101703806A true CN101703806A (en) | 2010-05-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910250313A Pending CN101703806A (en) | 2009-12-03 | 2009-12-03 | Porous hydroxyapatite/chitosan-gelatine composite material bracket |
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| CN (1) | CN101703806A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102406963A (en) * | 2011-11-02 | 2012-04-11 | 天津大学 | Multi-component bone tissue engineering scaffold material and preparation method thereof |
| CN104368045A (en) * | 2014-11-06 | 2015-02-25 | 四川大学 | Icariin controlled release chitosan/hydroxyapatite composite scaffold material and preparation method thereof |
| CN107998455A (en) * | 2018-01-31 | 2018-05-08 | 济宁学院 | Hydroxy apatite-base bone tissue engineering scaffold and its powder 3D printing method |
| IT201700022625A1 (en) * | 2017-02-28 | 2018-08-28 | Consiglio Nazionale Ricerche | FILTER FOR THE EXCHANGE OF HEAT AND HUMIDITY BY APPLICATION IN MEDICAL FIELD AND PROCEDURE FOR ITS PRODUCTION |
| CN113230447A (en) * | 2021-05-24 | 2021-08-10 | 河北工业大学 | Hemostatic repair material and preparation method thereof |
-
2009
- 2009-12-03 CN CN200910250313A patent/CN101703806A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102406963A (en) * | 2011-11-02 | 2012-04-11 | 天津大学 | Multi-component bone tissue engineering scaffold material and preparation method thereof |
| CN104368045A (en) * | 2014-11-06 | 2015-02-25 | 四川大学 | Icariin controlled release chitosan/hydroxyapatite composite scaffold material and preparation method thereof |
| CN104368045B (en) * | 2014-11-06 | 2016-05-18 | 四川大学 | Icariin control release type shitosan/hydroxyapatite compound support frame material and preparation method thereof |
| IT201700022625A1 (en) * | 2017-02-28 | 2018-08-28 | Consiglio Nazionale Ricerche | FILTER FOR THE EXCHANGE OF HEAT AND HUMIDITY BY APPLICATION IN MEDICAL FIELD AND PROCEDURE FOR ITS PRODUCTION |
| WO2018158684A1 (en) * | 2017-02-28 | 2018-09-07 | Consiglio Nazionale Delle Ricerche | Filter for the exchange of heat and moisture for application in the medical field and procedure for the production thereof |
| CN110709448A (en) * | 2017-02-28 | 2020-01-17 | 意大利国家研究委员会 | Filter for heat and moisture exchange for use in medical field and production procedure thereof |
| US11628602B2 (en) | 2017-02-28 | 2023-04-18 | Consiglio Nazionale Delle Ricerche | Filter for the exchange of heat and moisture for application in the medical field and procedure for the production thereof |
| CN107998455A (en) * | 2018-01-31 | 2018-05-08 | 济宁学院 | Hydroxy apatite-base bone tissue engineering scaffold and its powder 3D printing method |
| CN113230447A (en) * | 2021-05-24 | 2021-08-10 | 河北工业大学 | Hemostatic repair material and preparation method thereof |
| CN113230447B (en) * | 2021-05-24 | 2022-04-08 | 河北工业大学 | Hemostatic repair material and preparation method thereof |
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Application publication date: 20100512 |