CN101869269B - Method for preparing gelatinous enteromorpha polysaccharide - Google Patents
Method for preparing gelatinous enteromorpha polysaccharide Download PDFInfo
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- CN101869269B CN101869269B CN2010102245787A CN201010224578A CN101869269B CN 101869269 B CN101869269 B CN 101869269B CN 2010102245787 A CN2010102245787 A CN 2010102245787A CN 201010224578 A CN201010224578 A CN 201010224578A CN 101869269 B CN101869269 B CN 101869269B
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Abstract
The invention relates to a method for preparing gelatinous enteromorpha polysaccharide. In the method, enteromorpha is taken as a raw material and the gelatinous enteromorpha polysaccharide is prepared by the steps of hot water extraction, salt coagulation, acidification and desalination and dietary alkali neutralization. The method for preparing the gelatinous enteromorpha polysaccharide aims to make full use of enteromorpha resource, making waste profitable and relieving environment pollution caused by the enteromorpha. The method has the advantages of simple process, low production cost and contribution to industrial production. The prepared gelatinous enteromorpha polysaccharide has good taste, is convenient to eat and is edible after being dissolved into hot water to form gel-like gelatinous enteromorpha polysaccharide. Gel products with different gel intensities are obtained by adding different types of salt into the gelatinous enteromorpha polysaccharide.
Description
Technical field
The present invention relates to a kind of preparing gelatinous enteromorpha polysaccharide, belong to field of food industry.
Background technology
Enteromorpha is the algae of Chlorophyta, Ulvales Ulvaceae Enteromorpha, is commonly called as tongue bar, green laver etc., is edible and medicinal algae since ancient times.Enteromorpha is extensive in the coastal distribution of China, all can grow throughout the year.Because its reproduction speed is exceedingly fast, and causes disruption of ecological balance in the water easily, and water quality is worsened unusually, jeopardizes normal growth and the existence of fishes and shrimps shellfish.On the other hand, Enteromorpha is nutritious, is rich in carbohydrate, protein, crude fibre and mineral matter, also contains fat and vitamin simultaneously.Because coarse, the fishy smell overrich of Enteromorpha fiber, directly edible quality is not good.
Chinese invention patent ZL200610052806.0 discloses dry powder of gelatinous polysaccharide of aloe and preparation method and the preparation of being made up of aloe polysaccharide gel powder and hydroxypropyl; Chinese invention patent ZL 97110608.8 discloses employing calcium chloride and has used extractant, and calcium hypochlorite is made the method that precipitating reagent extracts water-soluble spirulina polyose; Chinese invention patent ZL 99113707.8 discloses a kind of carragheen-SKGM compound gel fixed microorganism carrier, and the application of this carrier in preparation ribonucleoside triphosphote such as adenosine triphosphate (ATP), GTP (GTP), cytidine triphosphate (CTP) (CTP) and uridine triphosphate production processes such as (UTP).Chinese invention patent ZL 02135275.5 discloses a kind of preparation method of carraoligose, and through KCl staging purifying, drying adds water and is made into the hydrosol with carragheen.It is the preparation method and the application of the edible food packaging film of matrix with the sea grass polysaccharide that Chinese invention patent ZL 200910017287 discloses a kind of; Adopt hot water hydro-oxidation sodium lixiviate sea grass polysaccharide, with a certain amount of sea grass polysaccharide immersion liquid, phosphatide; Converted starch; CMC, glycerine, gelatin etc. are made into film forming liquid.It is the method and the application of feedstock production gelation polysaccharide that domestic and international patent does not all relate to the Enteromorpha.
Summary of the invention
The present invention provides a kind of preparing gelatinous enteromorpha polysaccharide, is intended to make full use of the Enteromorpha resource, and it is turned waste into wealth, and alleviates its pollution to environment.
The preparing gelatinous enteromorpha polysaccharide that the present invention relates to is characterized in that following steps are arranged: the clean oven dry of Enteromorpha is pulverized in (1), soaks the back and extracts, centrifugal or filtration; (2) algae-residue adds water again and repeats to extract 2～3 times; (3) merge extract; (4) add salting liquid and make the extract mass concentration reach 5～25%, place 2～8h, get the yellow green polysaccharide gel; (5) supernatant liquor is removed in centrifugal or squeezing, adds hcl acidifying in the deposition, is adjusted to pH≤2, places 0.5～4h; (6) centrifugally slough free solution, taking precipitate is admixed edible base and is become pasty state, and pH7～pH9 places 0.5～2h; (7) after pulverizer draws pine, go into the drying machine drying, promptly get lurid gelatinous enteromorpha polysaccharide through pulverizing.But the sea grass polysaccharide rehydration of preparation becomes gel, is applied in the food industry as food additives.This preparation method is simple, and is easy to operate, and its raw material source is abundant.
It is 1:5～1:30 that the middle Enteromorpha of above-mentioned steps (1) is soaked solid-to-liquid ratio, and extracting temperature is 80 ℃～100 ℃, filters and uses 200 mesh sieves.
To add the solid-to-liquid ratio that water repeats to extract again be 1:3～1:5 to algae-residue in the above-mentioned steps (2).
The salting liquid that adds in the above-mentioned steps (4), used salt are one or several the mixture in potassium chloride, magnesium chloride, calcium chloride, iron chloride, the alchlor.
Concentration of hydrochloric acid in the above-mentioned steps (5) is 10%～20%, and addition is 3%～5% of the deposition quality.
Edible base in the above-mentioned steps (6) is one or more the mixture in sodium carbonate, sodium acid carbonate, the NaOH, can adopt solid base or its solution, and addition is 5%～15% of a sediment quality.
Centrifugal speed in the above-mentioned steps (1) (5) (6) is 2000 rev/mins～3000 rev/mins, time 10 min～30min.
The gelatinous enteromorpha polysaccharide of the present invention preparation adds 5～30 times water, 80 ℃～100 ℃ dissolve down after, be cooled to room temperature and promptly form gel.
To the resources characteristic of Enteromorpha, the present invention adopts salt cohesion-acidifying desalination-Jia alkali neutralisation to prepare gelatinous enteromorpha polysaccharide.Show that through experiment the sea grass polysaccharide that extracts according to the present invention can form gels with salts such as potassium chloride, magnesium chloride, calcium chloride, iron chloride, alchlors, and change the intensity that the concentration of salt can be regulated gel.In extract, add these salts, help method centrifugal or squeezing further commonly used and make its dehydration, needn't use the traditional organic solvent dehydration method always, and then simplify process conditions, reduce production costs.
Remarkable advantage of the present invention:
Salt cohesion-acidifying desalination-Jia alkali neutralisation that the present invention adopts prepares that gelatinous enteromorpha polysaccharide method technology is simple, and production cost is low, helps industrialization production.Prepared gelatinous enteromorpha polysaccharide mouthfeel is good; The gelatinous enteromorpha polysaccharide instant of preparation; Behind the adding hot water dissolving gel is edible; And can utilize and add different salts (as: potassium chloride, magnesium chloride, calcium chloride, iron chloride, alchlor), the gel product that obtains having different gel strengths.
The specific embodiment
Below be several instantiation of the present invention, further describe the present invention, but the present invention be not limited only to this.
Embodiment 1
Get and clean oven dry and pulverize, cross the Enteromorpha 5g of 30 mesh sieves, adds 5 times water logging bubble 4h after, extracted 2 hours 80 ℃ of water-baths, centrifugal, algae-residue adds 5 times water and extracts, and repeats to extract the merging extract 3 times; Add potassium chloride and make the extract mass concentration reach 5%, placement is spent the night, and gets the yellow green polysaccharide gel; Through the squeezer squeezing, remove supernatant liquor, add 10% hydrochloric acid 0.5mL acidifying in the deposition, be adjusted to pH≤2, place 4h; 3000 rev/mins of centrifugal 10min remove supernatant liquor, admix 10% sodium carbonate 8mL neutralization in the sediment and make it form pasty state, place 2h; Drying and crushing promptly gets lurid gelatinous enteromorpha polysaccharide.
Embodiment 2
Get and clean oven dry and pulverize, cross the Enteromorpha 5g of 40 mesh sieves, adds 30 times water logging bubble 0.5h after, extracted 0.5 hour 100 ℃ of water-baths, cross 200 mesh sieves, algae-residue adds 3 times water again and extracts, and repeats to extract the merging extract 2 times; Add potassium chloride and make its concentration reach 25%, place 2h, get the yellow green polysaccharide gel; 2000 rev/mins of centrifugal 10min remove supernatant liquor, add 20% hydrochloric acid 0.5mL acidifying, are adjusted to pH≤2, place 2h; 2000 rev/mins of centrifugal 10min remove supernatant liquor, admix 10% NaOH 5mL neutralization and make it form pasty state, place 0.5h; Drying and crushing promptly gets lurid gelatinous enteromorpha polysaccharide.
Embodiment 3
Get and clean oven dry and pulverize, cross the Enteromorpha 5g of 40 mesh sieves, adds 5 times water logging bubble 0.5h after, extracted 1 hour 90 ℃ of water-baths, algae-residue adds 5 times water and extracts, and repeats to extract 2 times, merges extract, 200 mesh sieves; Add magnesium chloride and make its mass concentration reach 5%, place 5h, get the yellow green polysaccharide gel; 3000 rev/mins of centrifugal 10min remove supernatant liquor, add 10% hydrochloric acid 0.5mL acidifying, are adjusted to pH≤2, place 4h; 3000 rev/mins of centrifugal 10min remove supernatant liquor, admix 15% sodium acid carbonate 10mL neutralization and make it form pasty state, place 2h; Drying and crushing promptly gets lurid gelatinous enteromorpha polysaccharide.
Embodiment 4
Get and clean oven dry and pulverize, cross the Enteromorpha 5g of 50 mesh sieves, adds 15 times water logging bubble 0.5h after, extracted 2 hours 80 ℃ of water-baths, algae-residue adds 5 times water and extracts, and repeats to extract 2 times, merges extract, 200 mesh sieves; Add calcium chloride and make its mass concentration reach 20%, place 8h, get the yellow green polysaccharide gel; 3000 rev/mins of centrifugal 10min remove supernatant liquor, add 10% hydrochloric acid 0.5mL acidifying, are adjusted to pH≤2, place 4h; 3000 rev/mins of centrifugal 10min remove supernatant liquor, admix sodium bicarbonate solid 2g neutralization and make it form pasty state, place 2h; Drying and crushing promptly gets lurid gelatinous enteromorpha polysaccharide.
Embodiment 5
Get and clean oven dry and pulverize, cross the Enteromorpha 5g of 40 mesh sieves, adds 30 times water logging bubble 0.5h after, extracted 1 hour 100 ℃ of water-baths, algae-residue adds 3 times water and extracts, and repeats to extract 2 times, merges extract, 200 mesh sieves; Adding calcium chloride, iron chloride (ratio is 1:1) make its mass concentration reach 20%, place 4h, get the yellow green polysaccharide gel; 3000 rev/mins of centrifugal 10min remove supernatant liquor, add 10% hydrochloric acid 0.5mL acidifying, are adjusted to pH≤2, place 4h; 3000 rev/mins of centrifugal 10min remove supernatant liquor, admix sodium carbonate solid 2g neutralization and make it form pasty state, place 2h; Drying and crushing promptly gets lurid gelatinous enteromorpha polysaccharide.
Embodiment 6
Get and clean oven dry and pulverize, cross the Enteromorpha 5g of 60 mesh sieves, adds 5 times water logging bubble 0.5h after, extracted 1 hour 100 ℃ of water-baths, algae-residue adds 5 times water and extracts, and repeats to extract 2 times, merges extract, 200 mesh sieves; Adding magnesium chloride, alchlor (ratio is 2:1) make its mass concentration reach 25%, place 3h, get the yellow green polysaccharide gel; 3000 rev/mins of centrifugal 10min remove supernatant liquor, add 10% hydrochloric acid 0.5mL acidifying, are adjusted to pH≤2, place 4h; 3000 rev/mins of centrifugal 10min remove supernatant liquor, admix sodium carbonate solid 2g neutralization and make it form pasty state, place 2h; Drying and crushing promptly gets lurid gelatinous enteromorpha polysaccharide.
Embodiment 7
Get the gelatinous enteromorpha polysaccharide of embodiment 1-6 preparation, add 5～30 times water, after dissolving under 80 ℃～100 ℃, be cooled to room temperature and promptly form gel.
Claims (6)
1. a preparing gelatinous enteromorpha polysaccharide is characterized in that: be raw material with the Enteromorpha, adopt in hot water lixiviate, salt cohesion, acidifying desalination, the dietary alkali and the preparation gelatinous enteromorpha polysaccharide; Said preparation method's concrete steps are following:
(1) Enteromorpha is cleaned oven dry and pulverizes, cross 30～60 mesh sieves, add water logging bubble 0.5～4h of 5～30 times after, extract 0.5～2h in 80 ℃～100 ℃, centrifugal or filter and obtain extract;
(2) algae-residue after centrifugal or filtration adds 3～5 times water again, under above-mentioned condition, repeats to extract 2～3 times;
(3) merge extract;
(4) add mass concentration 40%-90% salting liquid in the extract, make its mass concentration reach 5%～25%, place 2～8h, get the yellow green polysaccharide gel;
(5) supernatant liquor is removed in centrifugal or squeezing, adds hcl acidifying in the deposition, is adjusted to pH≤2, places 0.5～4h;
(6) centrifugal, and the collecting precipitation thing, sediment is admixed the edible base neutralization, makes it form pasty state, pH7～pH9, and place 0.5～2h;
(7) after pulverizer draws pine, carry out drying, pulverize then and promptly get lurid gelatinous enteromorpha polysaccharide.
2. preparing gelatinous enteromorpha polysaccharide according to claim 1 is characterized in that: said gelatinous enteromorpha polysaccharide, add 5～30 times water, and at 80 ℃～100 ℃ down after the dissolving, be cooled to room temperature and promptly form gel.
3. preparing gelatinous enteromorpha polysaccharide according to claim 1 is characterized in that: centrifugal centrifugal speed described in step (1), (5), (6) is 2000 rev/mins～3000 rev/mins, time 10 min～30min.
4. preparing gelatinous enteromorpha polysaccharide according to claim 1 is characterized in that: the salting liquid that adds in the step (4), used salt are one or several the mixture in potassium chloride, magnesium chloride, calcium chloride, iron chloride, the alchlor.
5. preparing gelatinous enteromorpha polysaccharide according to claim 1 is characterized in that: the hydrochloric acid mass concentration described in the step (5) is 10%～20%, and addition is 3%～5% of the deposition quality.
6. preparing gelatinous enteromorpha polysaccharide according to claim 1; It is characterized in that: the described edible base of step (6) is one or more the mixture in sodium carbonate, sodium acid carbonate, the NaOH; Can adopt solid base or its solution, addition is 5%～15% of a sediment quality.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102100322B (en) * | 2011-01-11 | 2012-06-27 | 福建农林大学 | Composite food glue containing enteromorpha gel polysaccharide and preparation method thereof |
| CN102144743B (en) * | 2011-01-11 | 2012-08-22 | 福建农林大学 | Enteromorpha prolifera jelly and preparation method thereof |
| CN102166015B (en) * | 2011-03-25 | 2012-12-19 | 宁波大学 | Preparation method of probiotics agent capable of lowering blood fat |
| CN102726771A (en) * | 2011-04-11 | 2012-10-17 | 株式会社海清净 | Manufacturing method of seaweed processing food |
| CN103255662B (en) * | 2013-05-06 | 2015-03-04 | 青岛大学 | Preparation method of nanometer fibril enteromorpha cellulose |
| CN104593451B (en) * | 2015-01-27 | 2018-11-23 | 青岛海洋生物医药研究院股份有限公司 | A method of preparing Enteromorpha acidic polysaccharose |
| CN106538843A (en) * | 2016-09-30 | 2017-03-29 | 江西中创汇智智能科技有限公司 | A kind of method that utilization Enteromorpha manufactures trace mineral supplement |
| CN109105871A (en) * | 2018-08-10 | 2019-01-01 | 青岛海莱美生物科技有限公司 | A kind of preparation method of sea lettuce gel |
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| CN101485343B (en) * | 2009-03-02 | 2013-06-05 | 中国海洋大学生物工程开发有限公司 | Extraction technology of active ingredients of enteromorpha and preparation method of enteromorpha seaweed fertilizer |
| CN101628647A (en) * | 2009-07-31 | 2010-01-20 | 刘畅 | Preparation and application of edible food packaging film rich in sea grass polysaccharide |
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