CN101869170A - Method for extracting proteins and chitin from shrimp heads and shrimp shells by pediococcus acidilactici - Google Patents
Method for extracting proteins and chitin from shrimp heads and shrimp shells by pediococcus acidilactici Download PDFInfo
- Publication number
- CN101869170A CN101869170A CN 201010181224 CN201010181224A CN101869170A CN 101869170 A CN101869170 A CN 101869170A CN 201010181224 CN201010181224 CN 201010181224 CN 201010181224 A CN201010181224 A CN 201010181224A CN 101869170 A CN101869170 A CN 101869170A
- Authority
- CN
- China
- Prior art keywords
- shrimp
- chitin
- fermentation
- pediococcus acidilactici
- head
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a method for extracting proteins and chitin from shrimp heads and shrimp shells by pediococcus acidilactici, which comprises the following steps: (1) selecting fresh or frozen shrimp heads and shrimp shells, and unfreezing the frozen shrimp heads and the frozen shrimp shells; (2) adding carbon sources into the shrimp heads and the shrimp shells; (3) inoculating the pediococcus acidilactici; (4) carrying out heat-insulation fermentation; (5) separating fermentation liquid from residue after the fermentation is completed, and obtaining protein powder after the fermentation liquid is dried; and (6) carrying out diluted acid soaking and decolourization on fermentation residue to obtain chitin. Through the method of the invention, the proteins and the chitin in the shrimp heads and the shrimp shells can be simultaneously extracted out, and the obtained hydrolyzed proteins do not have stinking odor, and have rich calcium lactate and other mineral substances. The method of the invention has the advantages of simple operation method, production cost reduction and environmental pollution reduction, and rich nutritional ingredients contained in the shrimp heads and the shrimp shells can be sufficiently utilized.
Description
Technical field
The present invention relates to from aquatic products, extract the method field of protein and chitin, be specifically related to a kind of method of from shrimp head and shrimp shell, extracting protein and chitin.
Background technology
Concerning shrimps, shrimp head and shrimp shell are rich in rich nutrient substances, for example the shrimp head of prawn, shrimp shell account for about 30% of shrimp body weight, wherein except containing mineral matters such as chitin, calcium carbonate, also contain compositions such as 7 ~ 15% protein, 2 ~ 4% greases, abundant vitamin E, astaxanthin, lutein, lecithin, nutritive value is higher.
The utilization of prawn head, shrimp shell at present is mainly aspect two: the one, be used to produce chitin, and the 2nd, utilize autologous tissue's protease or foreign protein enzyme hydrolysis protein wherein, its liquefaction is separated.But directly produce chitin, need acid, alkali-treated raw materials with high concentration with shrimp head, shrimp shell, not only seriously polluted, and protein wherein, fat, calcareous etc. all be removed as impurity, can't utilize.The hydrolyzate that method by protease hydrolytic obtains also all exists tangible bitter taste and stench flavor, and product special flavour is poor, can't be as the base-material of other food, can only make feed, in addition, be easy to take place corrupt in the hydrolytic process, and add protease and also improved production cost.
The present domestic similar report that utilizes Pediococcus acidilactici fermentation shrimp head, shrimp shell that do not have as yet, though a small amount of similarly research report is abroad arranged, but mainly be to utilize lactobacillus-fermented can produce the character of a large amount of lactic acid, the pH value of shrimp head, shrimp shell is reduced greatly, reach nature corrosion-resistant purpose, make raw material long-term storage at normal temperatures, be convenient to ensilage as animal.Be exactly to utilize lactobacillus-fermented that protein hydrolyzate in shrimp head, the shrimp shell is divided from coming out in addition, compositions such as little fat, astaxanthin also dissociate out simultaneously, the lactic acid that fermentation produces is also with the calcareous stripping in the shell, because shrimp head, the decalcification of shrimp shell, it is then easier to produce chitin with the fermentation residue that filters out.
As D.J.Evers and D.J.Carroll (D.J.Evers, D.J.Carroll.Ensiling salt-preservedshrimp waste with grass straw and molasses Animal Feed Science Technology.1998,71:241～249) studied to mix and utilize Lactobacillus plantarum and enterococcus faecalis and add molasses and straw fermentation shrimp head, shrimp shell are used to make animal feed.People (Neith Pacheco such as Neith Pacheco, M ó nicaGarnica-Gonz á lez, Jessica Y.Ram í rez-Hern á ndez, et al.Effect of temperature onchitin and astaxanthin recoveries from shrimp waste using lactic acid bacteria.Bioresource Technology, 2009,100:2849～2854) studied and utilize Lactobacillus plantarum fermented shrimp shell, shrimp head to reclaim astaxanthin and chitin, and studied the influence of temperature ferment effect; People such as N.M.Sachindra (N.M.Sachindra, N.Bhaskar, G.S.Siddegowda, et al.Recovery of carotenoidsfrom ensilaged shrimp waste[J] .Bioresource Technology, 2007,98:1642～1646) studied the problem of utilizing Lactobacillus plantarum from the zymotic fluid of shrimp shell, shrimp head, to reclaim carotenoid.People (Luis A.Cira such as Luis A.Cira, Sergio Huerta, George M.Hall, et al.Pilot scale lactic acidfermentation of shrimp wastes for chitin recovery.Process Biochemistry, 2002,37:1359～1366) studied the optimal conditions of fermentation of lactobacillus ferment shrimp shell, shrimp head, comprise inoculum concentration, add sugar type, sugaring amount, fermentation time etc., they adopt sucrose to make carbon source, inoculum concentration 5%, 30 ℃ of fermentation temperatures, fermenting, system pH can be reduced to 4.2 ~ 4.3 after 96 hours.People such as Choorit (Choorit, W., Patthanamanee, W., Manurakchinakorn, S., 2008.Use of response surface method forthe determination of demineralization efficiency in fermented shrimp shells.Bioresource Technology.99 6168-6173.) has studied with Pediococcus acidilactici microorganism belonging to genus fermentation shrimp head, shrimp shell recovery chitin and carotenoid.
Summary of the invention
The objective of the invention is to seriously polluted according to what exist in the existing method of from aquatic products, extracting protein and chitin, the problem of nutrient loss, a kind of method of extracting protein and chitin from shrimp head and shrimp shell is provided, this method can obtain not having stench flavor and be rich in calcium lactate from shrimp head and shrimp shell protein powder, also can obtain chitin simultaneously, method is simple fast, reduces environmental pollution, has made full use of the nutritional labeling that is rich in shrimp head and the shrimp shell.
The object of the invention is achieved by the following technical programs:
A kind of method of extracting protein and chitin from shrimp head and shrimp shell, this method are to adopt the Pediococcus acidilactici fermentation not only to extract from shrimp head and shrimp shell to obtain protein, also extract and obtain chitin.
The present invention adopts the Pediococcus acidilactici fermentation to extract simultaneously from shrimp head and shrimp shell and has obtained protein and chitin, and the high acid environment that utilizes the Pediococcus acidilactici fermentation to cause can prevent that shrimp head, shrimp shell from taking place in the self-dissolving process corrupt, thereby prolong the self-dissolving time greatly; Pediococcus acidilactici fermentation simultaneously can produce small amounts of protease, and these favorable factors have guaranteed the abundant hydrolysis of protein, improves extraction rate of protein; Utilize Pediococcus acidilactici can eliminate the stench flavor of zymotic fluid, improve the palatability of product; Utilize the lactic acid that fermentation produces can be with the insoluble calcium phosphate stripping in shrimp shell, the shrimp head, obtain the fermentation residue that protein, mineral matter are removed substantially, this fermentation residue can be used for further producing chitin.
The used Pediococcus acidilactici of the present invention is those skilled in the art's fermenting and producing varieties of food items probio commonly used, and sale is arranged on the market.
A kind of method of utilizing Pediococcus acidilactici to extract protein and chitin from shrimp head and shrimp shell of the present invention comprises the steps: that specifically (1) choose the shrimp head of fresh or cold storage, shrimp shell, and shrimp head, the shrimp shell of cold storage thaw; (2) in shrimp head, shrimp shell, add carbon source and mixing; (3) inoculating lactic acid sheet coccus; (4) heat-preservation fermentation; (5) after the fermentation ends zymotic fluid is separated with residue, the zymotic fluid drying obtains protein powder; (6) fermentation residue soaks and decolouring through diluted acid, obtains chitin.
As a kind of preferred version, in the above-mentioned steps (1), shrimp head and shrimp shell adopt raw material fresh or cold storage all can, but can not use dry raw material; Thawing of cold storage shrimp head, shrimp shell can be adopted various defreezing methods, as thaw naturally, hot-air thaws, flowing water thaws, microwave thawing, electrical heating thaw etc.; In order to reduce the volume of shrimp head, shrimp shell, be crushed to below the 1cm it better; For improving ferment effect, can also in shrimp head, shrimp shell, add an amount of water, the water that generally adds shrimp head, shrimp chitin amount 20% ~ 200% is better; For shortening the induction period of fermentation, can regulate the pH of shrimp head, shrimp shell with the acid that allows in food or the feed to use, reach between 6.0 ~ 6.8.
As a kind of preferred version, in the above-mentioned steps (2), the addition of carbon source may be selected to be shrimp head and more than 5% of shrimp shell gross mass, preferred 12% ~ 20%, these carbon sources comprise one or more the mixture in glucose, sucrose, maltose, dextrin, starch, starch syrup, HFCS, lactose, whey powder, the molasses.
As a kind of preferred version, in the above-mentioned steps (3), the inoculation of Pediococcus acidilactici adopts those skilled in the art's routine operation to carry out, and the inoculum concentration of Pediococcus acidilactici can be selected shrimp head and more than 0.1% of shrimp shell gross mass, preferred 3% ~ 8%.
As a kind of preferred version, in the above-mentioned steps (4), the temperature of heat-preservation fermentation can be selected 20 ~ 50 ℃, preferred 30 ~ 40 ℃; Fermentation time is more than the 24h, more than the preferred 120h.
As a kind of preferred version, in the above-mentioned steps (5), by centrifugal or filter method zymotic fluid is separated with fermentation residue after the fermentation ends; For removing the tart flavour of zymotic fluid, the alkali that can adopt those skilled in the art to use always is as adopting sodium carbonate or sodium acid carbonate neutralise broth; The drying means that the drying of zymotic fluid can adopt any one those skilled in the art to use always as adopting spray-drying, obtains powdered substance after the drying, be the protein of gained of the present invention.
As a kind of preferred version, in the above-mentioned steps (6), adopt diluted acid to remove relict mineral matter and decolouring processing chitin extraction, it is the practices well that those skilled in the art obtain chitin, the present invention during chitin extraction, selects diluted acid soaking fermentation residue from fermentation residue, can select various diluted acids, as watery hydrochloric acid, the concentration of diluted acid is high more, and soak time is long more, and the effect of demineralization is just good more, therefore the concentration of watery hydrochloric acid can be selected more than the 0.01mol/L preferred 0.2 ~ 0.5mol/L; Soak time can be selected more than the 0.5h, preferred 2 ~ 6h; Decolouring after diluted acid soaks is handled, adopt the decolouring processing operation that chitin extraction is used always in the prior art all can realize the present invention, as adopting conventional hydrogen peroxide decolouring, potassium permanganate decolouring or sunlight exposure etc. then can obtain required chitin at last from fermentation residue.
Compared with prior art, the present invention has following beneficial effect:
1. the present invention's high acid environment of utilizing Pediococcus acidilactici fermentation to cause can prevent that shrimp head, shrimp shell from corruption taking place in the self-dissolving process, thereby the self-dissolving time can be prolonged greatly, the small amounts of protease that produces in the prolongation of self-dissolving time and the Pediococcus acidilactici sweat, can guarantee the abundant hydrolysis and the high usage of protein, need not to add protease, peracid is also very favourable for the subsequent treatment and the preservation of product in addition again;
2. the present invention utilizes the lactic acid of fermentation generation the mineral matter major parts such as calcium salt in shrimp head, the shrimp shell can be removed, it is not only easier to produce chitin with the shrimp head behind the demineralization, shrimp shell, and can reduce the consumption of hydrochloric acid, reduce cost and reduce environmental pollution;
3. the present invention utilizes the Pediococcus acidilactici fermentation can eliminate the stench flavor of zymotic fluid, can improve the palatability of product, and zymotic fluid is rich in mineral matters such as calcareous;
4. the present invention adopts the Pediococcus acidilactici zymotechnique, and not only method of operating is simple, and low cost of raw materials, and can be simultaneously obtain protein and chitin from shrimp head and shrimp shell makes that contained composition is fully utilized in shrimp head and the shrimp shell.
The specific embodiment
Below in conjunction with specific embodiment the present invention is done explanation further, but specific embodiment is not done any qualification to the present invention.
Embodiment 1
Present embodiment utilizes the Pediococcus acidilactici fermentation to extract protein and chitin from shrimp head and shrimp shell, and its concrete steps are as follows:
(1) gets fresh shrimp head of 50g and shrimp shell, shrimp head and shrimp shell meal are broken to about 0.5cm, add 25mL water (shrimp head and shrimp shell gross mass 50%), regulate pH to 6.4;
(2) add 7.5g glucose (shrimp head and shrimp shell gross mass 15%) again;
(3) inoculating lactic acid sheet coccus, inoculum concentration are 5% of shrimp head and shrimp shell gross mass;
(4) in 35 ℃ of heat-preservation fermentations 5 days;
(5) after the fermentation ends, centrifugation zymotic fluid and fermentation residue; Zymotic fluid neutralizes through sodium carbonate, concentrates, and obtains powdered substance 7.2g after the spray-drying, and this powdered substance is present embodiment gained protein;
(6) fermentation residue to neutral, is put in the decolouring of being exposed to the sun under the daylight with the clear water rinsing after 0.5mol/L watery hydrochloric acid soaks 5h, obtains chitin.
Embodiment 2
Present embodiment utilizes the Pediococcus acidilactici fermentation to extract protein and chitin from shrimp head and shrimp shell, and its concrete steps are as follows:
(1) gets fresh shrimp head of 100g and shrimp shell, shrimp head and shrimp shell meal are broken to about 0.5cm, regulate pH to 6.5;
(2) add 20g glucose (shrimp head and shrimp shell gross mass 20%) again;
(3) inoculating lactic acid sheet coccus, inoculum concentration are 3% of shrimp head and shrimp shell gross mass;
(4) in 30 ℃ of heat-preservation fermentations 7 days;
(5) after the fermentation ends, centrifugation zymotic fluid and fermentation residue; Zymotic fluid neutralizes through sodium carbonate, concentrates, and obtains powdered substance 14.7g after the spray-drying, and this powdered substance is the protein of present embodiment gained;
(6) fermentation residue soaks 6h through 0.4mol/L watery hydrochloric acid, uses the clear water rinsing to neutral then, is put in the decolouring of being exposed to the sun under the daylight, obtains chitin.
Claims (7)
1. method of utilizing Pediococcus acidilactici to extract protein and chitin from shrimp head and shrimp shell is characterized in that comprising the steps: that (1) choose the shrimp head of fresh or cold storage, shrimp shell, and shrimp head, the shrimp shell of cold storage thaw; (2) in shrimp head, shrimp shell, add carbon source and mixing; (3) inoculating lactic acid sheet coccus; (4) heat-preservation fermentation; (5) after the fermentation ends zymotic fluid is separated with residue, the zymotic fluid drying obtains protein powder; (6) fermentation residue soaks and decolouring through diluted acid, obtains chitin.
2. the method for utilizing Pediococcus acidilactici from shrimp head and shrimp shell, to extract protein and chitin according to claim 1, the shrimp head, the thawing of shrimp shell that it is characterized in that cold storage described in the step (1) can be adopted various defreezing methods, comprise that nature thaws, hot-air thaws, flowing water thaws, microwave thawing, electrical heating thaw.
3. the method for utilizing Pediococcus acidilactici to extract protein and chitin from shrimp head and shrimp shell according to claim 1 is characterized in that carbon source described in the step (2) is one or more the mixture in glucose, sucrose, maltose, dextrin, starch, starch syrup, HFCS, lactose, whey powder, the molasses; The adding quality of carbon source is shrimp head and more than 5% of shrimp shell gross mass.
4. the method for utilizing Pediococcus acidilactici to extract protein and chitin from shrimp head and shrimp shell according to claim 1 is characterized in that in the step (3) that the inoculum concentration of described Pediococcus acidilactici is shrimp head and more than 0.1% of shrimp shell gross mass.
5. the method for utilizing Pediococcus acidilactici from shrimp head and shrimp shell, to extract protein and chitin according to claim 1, the temperature that it is characterized in that heat-preservation fermentation described in the step (4) is 20 ~ 50 ℃, fermentation time is more than the 24h.
6. the method for utilizing Pediococcus acidilactici to extract protein and chitin from shrimp head and shrimp shell according to claim 1 is characterized in that zymotic fluid described in the step (5) separates the method centrifugal or that filter that adopts with residue.
7. the method for utilizing Pediococcus acidilactici to extract protein and chitin from shrimp head and shrimp shell according to claim 1 is characterized in that the diluted acid of the immersion employing of fermentation residue described in the step (6) comprises various inorganic acids and organic acid; Dilute acid concentration reaches more than the 0.01mol/L with pH-meter, and soak time is more than the 0.5h; Hydrogen peroxide decolouring, potassium permanganate decolouring or sunlight exposure decolouring are adopted in the decolouring of fermentation residue.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010181224 CN101869170A (en) | 2010-05-18 | 2010-05-18 | Method for extracting proteins and chitin from shrimp heads and shrimp shells by pediococcus acidilactici |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010181224 CN101869170A (en) | 2010-05-18 | 2010-05-18 | Method for extracting proteins and chitin from shrimp heads and shrimp shells by pediococcus acidilactici |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101869170A true CN101869170A (en) | 2010-10-27 |
Family
ID=42994400
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010181224 Pending CN101869170A (en) | 2010-05-18 | 2010-05-18 | Method for extracting proteins and chitin from shrimp heads and shrimp shells by pediococcus acidilactici |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101869170A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103156049A (en) * | 2011-12-08 | 2013-06-19 | 上海红马饲料有限公司 | Method for extracting protein and chitin by fermenting shrimp heads and shrimp shells |
| CN104117418A (en) * | 2014-07-16 | 2014-10-29 | 华南农业大学 | Chitin crushing method |
| CN106967777A (en) * | 2017-05-11 | 2017-07-21 | 威海市四合生物科技有限公司 | A kind of fermented oyster shell prepares the zymotechnique of natural calcium lactate |
| CN110496849A (en) * | 2019-08-27 | 2019-11-26 | 岭南师范学院 | A kind of high-efficient treatment method of shrimp waste |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1338476A (en) * | 2001-09-07 | 2002-03-06 | 陈启松 | Chitin production system |
| CN101579037A (en) * | 2009-06-10 | 2009-11-18 | 华南农业大学 | Method for extracting proteins from heads and shells of prawns |
| CN101579132A (en) * | 2009-06-10 | 2009-11-18 | 华南农业大学 | Method for extracting proteins and chitin from heads and shells of prawns |
-
2010
- 2010-05-18 CN CN 201010181224 patent/CN101869170A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1338476A (en) * | 2001-09-07 | 2002-03-06 | 陈启松 | Chitin production system |
| CN101579037A (en) * | 2009-06-10 | 2009-11-18 | 华南农业大学 | Method for extracting proteins from heads and shells of prawns |
| CN101579132A (en) * | 2009-06-10 | 2009-11-18 | 华南农业大学 | Method for extracting proteins and chitin from heads and shells of prawns |
Non-Patent Citations (3)
| Title |
|---|
| 《Enzyme and microbail Technology》 20071231 Bhaskar 等 Shrimp biowaste fermentation with Pediococcus acidolactici CFR2182: 第40卷, 2 * |
| 《农产品加工·学刊》 20080131 段杉等 酶法回收虾头和虾壳中的蛋白质 43-46 , 第124期 2 * |
| 《食品与发酵工业》 20091231 段杉等 利用乳酸菌发酵协同自溶作用回收虾头、虾壳中的蛋白质 80-83 第35卷, 第2期 2 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103156049A (en) * | 2011-12-08 | 2013-06-19 | 上海红马饲料有限公司 | Method for extracting protein and chitin by fermenting shrimp heads and shrimp shells |
| CN104117418A (en) * | 2014-07-16 | 2014-10-29 | 华南农业大学 | Chitin crushing method |
| CN104117418B (en) * | 2014-07-16 | 2016-02-10 | 华南农业大学 | A kind of breaking method of chitin |
| CN106967777A (en) * | 2017-05-11 | 2017-07-21 | 威海市四合生物科技有限公司 | A kind of fermented oyster shell prepares the zymotechnique of natural calcium lactate |
| CN110496849A (en) * | 2019-08-27 | 2019-11-26 | 岭南师范学院 | A kind of high-efficient treatment method of shrimp waste |
| CN110496849B (en) * | 2019-08-27 | 2020-05-26 | 岭南师范学院 | Efficient processing method of shrimp waste |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101869171A (en) | Method for extracting proteins and chitin from shrimp heads and shrimp shells by lactobacillus plantarum | |
| CN101579132B (en) | Method for extracting proteins and chitin from heads and shells of prawns | |
| CN103156049B (en) | Method for extracting protein and chitin by fermenting shrimp heads and shrimp shells | |
| CN102550804B (en) | The preparation method of the forage protein of a kind of leftovers of tilapia and dregs of beans mixed culture fermentation | |
| CN104161259B (en) | A kind of breaking yeast cellule membrane also prepares the method for yeast extract | |
| CN103609862B (en) | Method for improving feeding nutritive value of sesame seed meal by combining enzymolysis method with fermentation method | |
| CN101768507B (en) | Method for extracting crude fish oil in leftovers of tilapia | |
| CN104892037A (en) | Method for producing water-soluble fish protein organic fertilizer from fish | |
| KR20190038588A (en) | Single cell protein from thermophilic fungi | |
| CN105494890A (en) | Enzymolysis and fermentation combined method for improving feeding nutritional value of rapeseed meal | |
| CN105624250A (en) | Enzymolysis-fermentation coupled aquatic protein active peptide preparation method | |
| CN102660436A (en) | Oyster yellow wine | |
| CN105039453A (en) | Method for preparing rice bran polysaccharides with improved oxidation resistance and application of rice bran polysaccharides | |
| CN106701606A (en) | Gene engineering candida utilis capable of degrading and utilizing kitchen waste and construction method of gene engineering candida utilis | |
| CN105624249A (en) | Preparation method of aquatic protein and plant protein compound active peptide | |
| CN102488087A (en) | Biological detoxification method for camellia seed cakes | |
| CN101579037B (en) | Method for extracting proteins from heads and shells of prawns | |
| CN101869170A (en) | Method for extracting proteins and chitin from shrimp heads and shrimp shells by pediococcus acidilactici | |
| CN102586378A (en) | Method for extracting substances from fermented shrimp head shells | |
| CN102119759B (en) | Method for extracting protein and chitosan from head and shell of shrimp by lactobacillus acidophilus | |
| CN101904405A (en) | Method for extracting protein and chitin from shrimp heads and shrimp shells by using edible acid | |
| CN101322541B (en) | Method for preparing delicate flavor agent using fresh water fish leftover bits and pieces | |
| CN105028926A (en) | Piglet edema prevention pig feed and preparation method thereof | |
| CN101720905B (en) | Flavor yeast albumen powder and preparation method thereof | |
| CN114946996B (en) | Citric acid mycelium residue liquid peptide feed and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20101027 |