CN101869077A - Tissue culture and seedling raising method for zinc cadmium hyperaccumulator plant Sedum plumbizincicola - Google Patents
Tissue culture and seedling raising method for zinc cadmium hyperaccumulator plant Sedum plumbizincicola Download PDFInfo
- Publication number
- CN101869077A CN101869077A CN 201010228740 CN201010228740A CN101869077A CN 101869077 A CN101869077 A CN 101869077A CN 201010228740 CN201010228740 CN 201010228740 CN 201010228740 A CN201010228740 A CN 201010228740A CN 101869077 A CN101869077 A CN 101869077A
- Authority
- CN
- China
- Prior art keywords
- medium
- seedling
- tissue cultivating
- sedum plumbizincicola
- stem
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a high-frequency plant regeneration technique for tissue culture of repairing plant Sedum plumbizincicola, which comprises the following steps: respectively taking the stem segment, the stem tip and the leaf of the repairing plant Sedum plumbizincicola for tissue culture and propagation, and respectively establishing high-frequency regeneration systems for the stem segment, the stem tip and the leaf. Since the stem segment, the stem tip and the leaf of the Sedum plumbizincicola are directly selected during propagation, the materials can be obtained easily, the scope of the materials for the propagation of the Sedum plumbizincicola is widened, the propagation method is rapid and high-efficiency, and conditions are provided for physiological biochemical researches and molecular biological researches. Through respectively establishing the high-frequency plant regeneration systems for the stem segment, the stem tip or the leaf, the method is suitable for the screening and the micropropagation of somatic cell mutants and the transformation of the transgenic materials through agrobacterium-mediated transformation, and provides basic conditions for elaborating the heavy metal resistance of the Sedum plumbizincicola and the molecular biological mechanism of hyperaccumulators.
Description
Technical field
The present invention relates to stem section, stem apex or the blade cultured in vitro high-frequency plant regeneration technique of rehabilitation plant companion ore deposit red-spotted stonecrop, belong to method for plant tissue culture.
Background technology
Along with the modernization of industrial expansion and agricultural production, heavy metal pollution problem is on the rise in soil-plant-environmental system.Heavy metal pollution not only reduces crop yield and quality, cause the degeneration of soil function and quality, influence the healthy and sustainable development of agricultural production, and by runoff and leaching polluted surface water and underground water, worsen hydrological environment, and may jeopardize human life and health by approach such as direct contact, food chains.The agricultural land soil of China should be come into one's own by the improvement of heavy metal pollution at present.
The physico chemistry recovery technique that adopts (comprises the isolation embedding techniques, solidify stabilization technique, chemically stable technology and electric repairing technique etc.) soil pollution of improvement heavy metal, do not reach essentially and remove the purpose of polluting, just heavy metal is shifted or is fixed in the soil, reduce the amount that heavy metal enters plant ecosystem.And, use the often costly of these physico-chemical processes, and used chemical agent also may cause the secondary pollution of soil.And phytoremediation is to utilize a kind of new Environmental Biotechnology of heavy metal hyperaccumulative or the soil pollution of patience plant in treating, and it is that cheap, the no secondary of a kind of expense is polluted, and economizes the land resource, the green approach of environmental friendliness and sustainable development.The key of phytoremediation be to seek or cultivate can restrain oneself with tired one or more heavy metals of ultraproduct, biomass is big, adaptability is wide and breed distinguished germ plasm resource fast.Rehabilitation plant companion ore deposit red-spotted stonecrop (Sedum plumbizincicola X.H.Guo et S.B.Zhou sp.nov.) is a heavy metal species patience and the super enriching plant that China has just found, happiness is born in and is rich in Pb, area, Zn ore deposit, perennial meat draft.Its habit of growth is similar substantially to the habit of growth of other red-spotted stonecrop, and natural distribution is in zhejiang and other places, but in the most of area of China equal normal growths, wide adaptability, biomass is big, fast growth.Be applicable to the contaminated environment phytoremediation technology, but the molecular biosciences mechanism of its heavy metal patience and super enrichment is not research as yet, reaching its maturity of Protocols in Molecular Biology opened up new approach for the molecule mechanism of studying its heavy metal patience and super enrichment, and this method is used for its micropropagation and somatic mutants screening and molecular biology research.So far, tissue culture technique does not appear in the newspapers on the red-spotted stonecrop of companion ore deposit as yet, therefore invent the method for tissue culture of this rehabilitation plant companion ore deposit red-spotted stonecrop, molecule mechanism to research companion's ore deposit red-spotted stonecrop heavy metal patience and super enrichment has great importance, simultaneously to promoting the contaminated environment phytoremediation technology to apply significant.
Summary of the invention
Technical problem: the purpose of this invention is to provide the high-frequency plant regeneration technique of a kind of rehabilitation plant companion ore deposit red-spotted stonecrop tissue culture, for the molecular biosciences mechanism of setting forth companion's ore deposit red-spotted stonecrop heavy metal patience and super enrichment is opened up new approach.Rehabilitation plant companion of the present invention ore deposit red-spotted stonecrop tissue culture technique has three kinds of schemes such as stem-tip tissue cultivation, blade callus induction, stem section callus induction.
Technical scheme: technical solution of the present invention is: the tissue cultivating and seedling method of zinc cadmium hyperaccumulator plant Sedum plumbizincicola, adopt stem-tip tissue to induce differential method, to get 0.3～0.5cm stem section or the stem apex that has axillalry bud after the red-spotted stonecrop sterilization of companion ore deposit, be inoculated on the inducing culture, keeping light intensity every day is after cultivating 20～40 days in 16 hours the incubator of illumination of 3000 luxs, take out the long stem apex that fascicular bud is arranged, choose big bud, be transferred on the strong seedling culture base, keeping light intensity every day is to cultivate for 3～8 weeks in 16 hours the incubator of illumination of 3000 luxs, obtain containing the tissue cultivating seedling of the long young root of many 2～3cm, then blake bottle is moved on to outdoorly, open bottleneck, pouring distilled water was placed after 2～3 days, water flush away medium is transplanted to the organic matrix of the fully nutrient of pH 6.0～8.0, and 2～4 weeks obtained tissue cultivating seedling.Above-mentioned inducing culture is the MS medium that contains (0.05～0.15) mg/L 6-BA and (1.5～2.5) mg/L NAA.Above-mentioned strong seedling culture base is the 1/2MS medium, wherein contains concentration and is (0.1～0.5) mg/L6-BA, (1.5～2.0) mg/L NAA, 5 μ mol/L Cd (NO
3)
2(0.1～1.5) mg/L IBA.
The tissue cultivating and seedling method of zinc cadmium hyperaccumulator plant Sedum plumbizincicola, adopt stem section callus induction method, to accompany ore deposit red-spotted stonecrop stem sterilization, cut leaf and lateral bud and terminal bud, be cut into the segment of 0.3～0.5cm, be inoculated on the inducing culture, 3～6 all evoked callus in the incubator of unglazed photograph, then callus is transferred in the differential medium, keeping light intensity every day is to cultivate 40～50 days in 16 hours the incubator of illumination of 3000 luxs, it is high and have the tissue cultivating seedling of the above young root of 1cm to obtain 3～5cm, then blake bottle is moved on to outdoorly, opens bottleneck, pouring distilled water was placed after 2～3 days, water flush away medium is transplanted to the organic matrix of the fully nutrient of pH 6.0～8.0, and 2～4 weeks obtained tissue cultivating seedling.Above-mentioned inducing culture is the MS medium, wherein contains concentration and is (0.1～0.5) mg/L 6-BA and (1.5～2.0) mg/L 2,4-D.Above-mentioned differential medium is the MS medium, wherein contains concentration and is (0.1～0.5) mg/L 6-BA and (0.1～0.5) mg/L 2,4-D.
The tissue cultivating and seedling method of zinc cadmium hyperaccumulator plant Sedum plumbizincicola adopts blade callus induction method, will accompany ore deposit red-spotted stonecrop blade sterilization, cuts leaf margin, vein and petiole, is cut into 0.2～0.3cm
2Small pieces, be inoculated on the inducing culture, 4～8 all evoked callus in the incubator of unglazed photograph, then callus is transferred in the differential medium, keeping light intensity every day is to cultivate 50～70 days in 16 hours the incubator of illumination of 3000 luxs, it is high and have a tissue cultivating seedling of the above young root of 1cm to obtain 3～5cm, then blake bottle is moved on to outdoor, open bottleneck, pouring distilled water was placed after 2～3 days, water flush away medium is transplanted to the organic matrix of the fully nutrient of pH 6.0～8.0, and 2～4 weeks obtained tissue cultivating seedling.Above-mentioned inducing culture is the MS medium, wherein contains concentration and is (2.5～3.5) mg/L NAA and (0.5～1.0) mg/L TDZ.Above-mentioned differential medium is the MS medium, wherein contains concentration and is (0.1～0.5) mg/L 6-BA and (0.1～0.5) mg/L 2,4-D.
Above-mentioned medium is sterilized medium, adjusts pH value to 5.5～5.8 with sodium hydroxide and hydrochloric acid before autoclaving; Cultivation temperature is 25 ± 1 ℃ in the cultured in vitro process.
Beneficial effect: stem section, stem apex, the blade that the present invention gets rehabilitation plant companion ore deposit red-spotted stonecrop respectively carries out tissue culture and expands numerously, set up stem section, stem apex, blade high frequency regenerating system simultaneously respectively.Owing to directly choose stem section, stem apex, the blade of companion ore deposit red-spotted stonecrop during breeding, draw materials easily, widened the scope of selecting material of companion ore deposit red-spotted stonecrop breeding, propagation method is quick, and is efficient, for Physiology and biochemistry, molecular biology research provide condition.The present invention seeks to method by the high-frequency plant regenerating system of setting up stem apex, stem section or blade respectively, suitable screening and micropropagation and agrobacterium-mediated transformation transgenosis converting material as somatic mutants are for the molecular biosciences mechanism of setting forth its heavy metal patience and super enrichment provides basic condition.
Embodiment
Below in conjunction with instantiation, further set forth the present invention.Should be understood that these embodiment only are used for that the present invention will be described, do not constitute the restriction to the claim scope, other alternative means that it may occur to persons skilled in the art that are all in claim scope of the present invention.
The implication of 1/2MS medium is that all the components in the MS medium reduces by half in the specification.
The said MS medium composition of the present invention sees Table 1:
Table 1MS medium constituent
| Composition | Working concentration (mg/L) |
| Potassium nitrate | ??1900 |
| Ammonium nitrate | ??1650 |
| Potassium dihydrogen phosphate | ??170 |
| Magnesium sulfate | ??370 |
| Calcium chloride | ??440 |
| Potassium iodide | ??0.83 |
| Boric acid | ??6.2 |
| Manganese sulphate | ??22.3 |
| Zinc sulphate | ??8.6 |
| Sodium molybdate | ??0.25 |
| Copper sulphate | ??0.025 |
| Cobalt chloride | ??0.025 |
| Disodium ethylene diamine tetraacetate | ??37.3 |
| Ferrous sulfate | ??27.8 |
| Inositol | ??100 |
| Glycine | ??2.0 |
| Thiamine hydrochloride | ??0.10 |
| Puridoxine hydrochloride | ??0.50 |
| Nicotinic acid | ??0.50 |
| Sucrose | ??30g/L |
| Agar | ??7-8g/L |
Embodiment 1:
To take from the stem section or the stem apex of rehabilitation plant companion ore deposit red-spotted stonecrop band axillalry bud on the zinc cadmium ore deposit rinses well with running water, with 70%vt alcohol immersion 50 seconds, use 0.1% (g/mL) mercuric chloride to soak again 10 minutes, outwell behind the mercuric chloride with after the sterile water wash 5 times, obtain on superclean bench, getting 0.3～0.5cm stem section or the stem apex that has axillalry bud after the aseptic seedling, being inoculated into, the bottled 50mL pH of the also cooled 200mL glass cultivation of autoclaving is 5.8 inducing cultures, (composition is the MS medium to 100 bottles inducing culture, wherein contain concentration and be (0.05～0.15) mg/L 6-BA and (1.5～2.5) mg/L NAA) 3 stem sections of every bottle graft kind or stem apex, with blake bottle put to temperature be 25 ± 1 ℃, keeping light intensity every day is to cultivate in 16 hours the incubator of illumination of 3000 luxs (Lux) after 25 days, take out the long stem apex that fascicular bud is arranged, choose big bud, being transferred to sterilized glass cultivates on the strong seedling culture base of bottled 50mL, the strong seedling culture base is that pH is 5.8 1/2MS medium, and wherein containing concentration is 0.1～0.5) mg/L 6-BA, (1.5～2.0) mg/L NAA, 5 μ mol/L Cd (NO
3)
2(0.1～1.5) mg/L IBA, blake bottle being put into temperature is 25 ± 1 ℃ again, keeping light intensity every day is to cultivate for 5～8 weeks in 16 hours the incubator of illumination of 3000 luxs (Lux), obtain containing the tissue cultivating seedling of the long young root of many 2～3cm, then blake bottle is moved on to outdoor, open bottleneck, adding a little distilled water placed after 2～3 days, wash the root medium, transplant organic matrix (production of Wuwei County, Anhui Province flower fertilizer factory) respectively, obtain the about 5cm tissue cultivating seedling of sturdy overground part after 2～4 weeks to fully nutrient.
Embodiment 2:
To take from the zinc cadmium ore deposit rehabilitation plant companion ore deposit red-spotted stonecrop stem section rinses well with running water, with 70%vt alcohol immersion 50 seconds, use 0.1% (g/mL) mercuric chloride to soak again 10 minutes, outwell behind the mercuric chloride with after the sterile water wash 5 times, obtain after the aseptic seedling after on the superclean bench stem section being cut blade and lateral bud, be cut into the segment of 0.3～0.5cm, being inoculated into, the bottled 50mL pH of the also cooled 200mL glass cultivation of autoclaving is on 5.5～5.8 inducing cultures, the inducing culture composition is the MS medium, wherein contain concentration and be (0.1～0.5) mg/L 6-BA and (1.5～2.0) mg/L 2,4-D, it is 25 ± 1 ℃ that blake bottle is put into temperature, obtain callus after 3～6 weeks in the incubator of unglazed photograph, it is in 5.8 differential mediums that callus is transferred to the bottled 50mLpH of sterilized glass cultivation, wherein the composition of 50 bottles of differential mediums is MS medium, wherein contain concentration and be (0.1～0.5) mg/L 6-BA and (0.1～0.5) mg/L 2,4-D, blake bottle being put into temperature is 25 ± 1 ℃ again, keeping light intensity every day is to cultivate 40 days in 16 hours the incubator of illumination of 3000 luxs (Lux), obtain the tissue cultivating seedling that the 3cm height has the above young root of 1cm, then blake bottle is moved on to outdoor, open bottleneck, adding a little distilled water placed after 2 days, transplant organic matrix (production of Wuwei County, Anhui Province flower fertilizer factory) behind the water flush away medium respectively, obtain the about 5cm tissue cultivating seedling of sturdy overground part after 4 weeks to fully nutrient.
Embodiment 3:
To take from the zinc cadmium ore deposit rehabilitation plant companion ore deposit red-spotted stonecrop blade rinses well with running water, with 70%vt alcohol immersion 50 seconds, use 0.1% (g/mL) mercuric chloride to soak again 10 minutes, outwell behind the mercuric chloride with after the sterile water wash 5 times, obtain after the aseptic seedling on superclean bench blade, after cutting leaf margin, be cut into 0.3～0.5cm
2Small pieces, being inoculated into, the bottled 50mL pH of the also cooled 200mL glass cultivation of autoclaving is on 5.5～5.8 inducing cultures, the inducing culture composition is the MS medium, wherein contain concentration and be (2.5～3.5) mg/L NAA and (0.5～1.0) mg/L TDZ, it is 25 ± 1 ℃ that blake bottle is put into temperature, obtain callus after 4～8 weeks in the incubator of unglazed photograph, it is in 5.8 differential mediums that callus is transferred to the bottled 50mLpH of sterilized glass cultivation, wherein the composition of 50 bottles of differential mediums is MS medium, wherein contain concentration and be (0.1～0.5) mg/L 6-BA and (0.1～0.5) mg/L 2,4-D, blake bottle being put into temperature is 25 ± 1 ℃ again, keeping light intensity every day is to cultivate 50～70 days in 16 hours the incubator of illumination of 3000 luxs (Lux), obtain the tissue cultivating seedling that the 3cm height has the above young root of 1cm, then blake bottle is moved on to outdoor, open bottleneck, adding a little distilled water placed after 2 days, transplant organic matrix (production of Wuwei County, Anhui Province flower fertilizer factory) behind the water flush away medium respectively, obtain the about 5cm tissue cultivating seedling of sturdy overground part after 4 weeks to fully nutrient.
Claims (10)
1. the tissue cultivating and seedling method of zinc cadmium hyperaccumulator plant Sedum plumbizincicola, it is characterized in that adopting stem-tip tissue to induce differential method: get 0.3～0.5cm stem section or the stem apex that has axillalry bud after will accompanying ore deposit red-spotted stonecrop sterilization, be inoculated on the inducing culture, keeping light intensity every day is after cultivating 20～40 days in 16 hours the incubator of illumination of 3000 luxs, take out the long stem apex that fascicular bud is arranged, choose big bud, be transferred on the strong seedling culture base, keeping light intensity every day is to cultivate for 3～8 weeks in 16 hours the incubator of illumination of 3000 luxs, obtain containing the tissue cultivating seedling of the long young root of many 2～3cm, then blake bottle is moved on to outdoor, open bottleneck, pouring distilled water was placed after 2～3 days, water flush away medium, transplant to the organic matrix of the fully nutrient of pH 6.0～8.0,2～4 weeks obtained tissue cultivating seedling.
2. the tissue cultivating and seedling method of zinc cadmium hyperaccumulator plant Sedum plumbizincicola according to claim 1 is characterized in that said inducing culture is the MS medium that contains (0.05～0.15) mg/L 6-BA and (1.5～2.5) mg/LNAA.
3. the tissue cultivating and seedling method of zinc cadmium hyperaccumulator plant Sedum plumbizincicola according to claim 1, it is characterized in that said strong seedling culture base is the 1/2MS medium, wherein contain concentration and be (0.1～0.5) mg/L6-BA, (1.5～2.0) mg/L NAA, 5 μ mol/L Cd (NO
3)
2(0.1～1.5) mg/L IBA.
4. the tissue cultivating and seedling method of zinc cadmium hyperaccumulator plant Sedum plumbizincicola, it is characterized in that adopting stem section callus induction method: will accompany ore deposit red-spotted stonecrop stem sterilization, cut leaf and lateral bud and terminal bud, be cut into the segment of 0.3～0.5cm, be inoculated on the inducing culture, 3～6 all evoked callus in the incubator of unglazed photograph, then callus is transferred in the differential medium, keeping light intensity every day is to cultivate 40～50 days in 16 hours the incubator of illumination of 3000 luxs, it is high and have a tissue cultivating seedling of the above young root of 1cm to obtain 3～5cm, then blake bottle is moved on to outdoor, open bottleneck, pouring distilled water was placed after 2～3 days, water flush away medium, transplant to the organic matrix of the fully nutrient of pH 6.0～8.0,2～4 weeks obtained tissue cultivating seedling.
5. the tissue cultivating and seedling method of zinc cadmium hyperaccumulator plant Sedum plumbizincicola according to claim 4 is characterized in that said inducing culture is the MS medium, wherein contains concentration and is (0.1～0.5) mg/L 6-BA and (1.5～2.0) mg/L 2,4-D.
6. the tissue cultivating and seedling method of zinc cadmium hyperaccumulator plant Sedum plumbizincicola according to claim 4 is characterized in that said differential medium is the MS medium, wherein contains concentration and is (0.1～0.5) mg/L 6-BA and (0.1～0.5) mg/L 2,4-D.
7. the tissue cultivating and seedling method of zinc cadmium hyperaccumulator plant Sedum plumbizincicola is characterized in that adopting blade callus induction method: will accompany ore deposit red-spotted stonecrop blade sterilization, and cut leaf margin, vein and petiole, and be cut into 0.2～0.3cm
2Small pieces, be inoculated on the inducing culture, 4～8 all evoked callus in the incubator of unglazed photograph, then callus is transferred in the differential medium, keeping light intensity every day is to cultivate 50～70 days in 16 hours the incubator of illumination of 3000 luxs, it is high and have a tissue cultivating seedling of the above young root of 1cm to obtain 3～5cm, then blake bottle is moved on to outdoor, open bottleneck, pouring distilled water was placed after 2～3 days, water flush away medium is transplanted to the organic matrix of the fully nutrient of pH 6.0～8.0, and 2～4 weeks obtained tissue cultivating seedling.
8. the tissue cultivating and seedling method of zinc cadmium hyperaccumulator plant Sedum plumbizincicola according to claim 7 is characterized in that said inducing culture is the MS medium, wherein contains concentration and is (2.5～3.5) mg/L NAA and (0.5～1.0) mg/L TDZ.
9. the tissue cultivating and seedling method of zinc cadmium hyperaccumulator plant Sedum plumbizincicola according to claim 7 is characterized in that said differential medium is the MS medium, wherein contains concentration and is (0.1～0.5) mg/L 6-BA and (0.1～0.5) mg/L 2,4-D.
10. the medium in above-mentioned arbitrary claim is sterilized medium, adjusts pH value to 5.5～5.8 with sodium hydroxide and hydrochloric acid before autoclaving; Cultivation temperature is 25 ± 1 ℃ in the cultured in vitro process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102287402A CN101869077B (en) | 2010-07-15 | 2010-07-15 | Tissue culture and seedling raising method for zinc cadmium hyperaccumulator plant Sedum plumbizincicola |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010102287402A CN101869077B (en) | 2010-07-15 | 2010-07-15 | Tissue culture and seedling raising method for zinc cadmium hyperaccumulator plant Sedum plumbizincicola |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101869077A true CN101869077A (en) | 2010-10-27 |
| CN101869077B CN101869077B (en) | 2012-03-14 |
Family
ID=42994310
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010102287402A Expired - Fee Related CN101869077B (en) | 2010-07-15 | 2010-07-15 | Tissue culture and seedling raising method for zinc cadmium hyperaccumulator plant Sedum plumbizincicola |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101869077B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106670219A (en) * | 2017-01-05 | 2017-05-17 | 湖南永清环保研究院有限责任公司 | Method for enhancing phytoremediation for cadmium pollution in soil |
| CN106811481A (en) * | 2017-03-31 | 2017-06-09 | 中国科学院植物研究所 | A kind of genetic transforming method of sengreen |
| CN112997881A (en) * | 2021-02-02 | 2021-06-22 | 中信建设有限责任公司 | Sedum aizoon explant sterilization method, callus induction culture medium and induction method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101015833A (en) * | 2007-02-16 | 2007-08-15 | 中国科学院南京土壤研究所 | Renovation method for plant in soil of zinc-cadmium combined pollution |
-
2010
- 2010-07-15 CN CN2010102287402A patent/CN101869077B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101015833A (en) * | 2007-02-16 | 2007-08-15 | 中国科学院南京土壤研究所 | Renovation method for plant in soil of zinc-cadmium combined pollution |
Non-Patent Citations (2)
| Title |
|---|
| 《土壤》 20061030 吴龙华等 中国景天科植物一新种--伴矿景天 第632-633页 1-10 第38卷, 第5期 2 * |
| 《土壤学报》 20090715 李娜等 收获方式对污染土壤上伴矿景天锌镉吸收性的影响 第725-728页 1-10 第46卷, 第4期 2 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106670219A (en) * | 2017-01-05 | 2017-05-17 | 湖南永清环保研究院有限责任公司 | Method for enhancing phytoremediation for cadmium pollution in soil |
| CN106811481A (en) * | 2017-03-31 | 2017-06-09 | 中国科学院植物研究所 | A kind of genetic transforming method of sengreen |
| CN106811481B (en) * | 2017-03-31 | 2019-12-24 | 中国科学院植物研究所 | Genetic transformation method of sedum plants |
| CN112997881A (en) * | 2021-02-02 | 2021-06-22 | 中信建设有限责任公司 | Sedum aizoon explant sterilization method, callus induction culture medium and induction method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101869077B (en) | 2012-03-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101297635B (en) | Method for breeding spore of Dryopteris varia | |
| CN102144547A (en) | Method for quickly breeding and transplanting grape stock unit | |
| CN109220810B (en) | Method for efficiently rooting peony embryos under aseptic condition | |
| CN101444185B (en) | Method for breeding heavy metal hyperaccumulative plant Sedum alfredii Hance germchit | |
| CN112189566B (en) | Rapid breeding method of cherry seedlings for stocks | |
| CN101455179B (en) | Tissue culture method of aged Sinojackia xylocarpa | |
| Shahid et al. | Morphogenic responses of Rauvolfia tetraphylla L. cultures to Cu, Zn and Cd ions | |
| CN1973617B (en) | Germchit propagation process of Alfred stonecrop as heavy metal super accumulating plant | |
| CN104823852B (en) | A kind of Herba Dendrobii tip of a root tissue culture quick propagation educates method | |
| CN103155862B (en) | Sinocalamus latiflorus flower pesticide inductor embryo the method obtaining regeneration plant | |
| CN100462001C (en) | Method for fast breeding water caltrop seed and seedling | |
| CN101869077B (en) | Tissue culture and seedling raising method for zinc cadmium hyperaccumulator plant Sedum plumbizincicola | |
| CN104663439B (en) | Tissue culture and rapid propagation method of waterweed | |
| CN106973796A (en) | A kind of tissue cultivating and seedling method of Idesia polycarpa | |
| CN1742563A (en) | Method for fast breeding water caltrop seedlings | |
| CN103548695B (en) | A kind of meadowrueleaf corydalis root quick breeding method for tissue culture | |
| CN112868527A (en) | Method for rapidly propagating flamingo pepper grass | |
| CN106465680B (en) | Rapid celery tissue culture system | |
| CN102668991B (en) | Application of penicillin to simple test-tube breeding of grapes and novel technology for test-tube breeding of grapes | |
| CN1164166C (en) | Tissue culture seedling propagation method for bramble Turamine, Xiumeite and blackberry black babesi | |
| JP2005052042A (en) | Method for producing seedling of rhodophyta alga | |
| CN112400696B (en) | Tissue culture method of evergreen common selfheal fruit-spike bamboo | |
| CN104604692A (en) | Myriophyllum aquaticum tissue culture and rapid propagation method thereof | |
| CN106879462A (en) | A kind of Ranalisma rostratum micro-propagation method | |
| CN104054579B (en) | A kind of method of tung oil tree petiole directly regenerated plant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180409 Address after: 210046 room 1206, C building, Xingzhi science and Technology Park, Nanjing economic and Technological Development Zone, Jiangsu Patentee after: JIANGSU FIREFLY ENVIRONMENTA SCIENCE TECHNOLOGY CO.,LTD. Address before: 210008 Xuanwu District, Jiangsu, Beijing East Road, No. 71, No. Patentee before: Institute of Soil Science, Chinese Academy of Sciences |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120314 |