CN101864470B - Method for increasing yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells - Google Patents
Method for increasing yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells Download PDFInfo
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- CN101864470B CN101864470B CN 201010197762 CN201010197762A CN101864470B CN 101864470 B CN101864470 B CN 101864470B CN 201010197762 CN201010197762 CN 201010197762 CN 201010197762 A CN201010197762 A CN 201010197762A CN 101864470 B CN101864470 B CN 101864470B
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Abstract
The invention belongs to the field of the submerged fermentation of microalgae cells, in particular to a method for increasing the yield of extracellular polysaccharide produced by the submerged fermentation of long thread moss cells. The method for increasing the yield of the extracellular polysaccharide of the long thread moss cells by controlling illumination intensity by stages and by a culture medium fermentation process has simple process and high production efficiency and is suitable for amplifying industrial production, and the polysaccharide of the long thread moss cells obtained by production can be used as a natural antiviral medicine or a food raw material after being separated and purified.
Description
(1) technical field
The invention belongs to the submerged fermentation field of microalgae cell, particularly a kind of method that improves yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells.
(2) background technology
Delivering vegetables is the economic blue-green algae of a kind of land natural disposition that is grown in NORTHWEST CHINA section and northern arid area, has very high nutritive value.Because its growing environment is abominable, hair weeds cells to a large amount of gelatinoid of cell exocrine, is wrapped in outside hair weeds cells and the filament in process of growth, forms gelatinous sheath, and the protection hair weeds cells is not subjected to the harm of arid, high temperature, uv-radiation.The main component of colloid is polysaccharide, and these polysaccharide materials are resisted vegetatively poor environment to delivering vegetables, and ensures that the normal growth of hair weeds cells has important biological meaning.
Research finds that the multiple viruses with big envelope such as the acidic polysaccharose that extracts-nosoflan infected by influenza, human cytomegalic inclusion disease virus, monospore exanthema virus have very strong antiviral activity, are a kind of potential antiviral from wild hair weeds cells; And the Nostoc flagelliforme Polysaccharides of liquid submerged fermentation production has high similarity with the natural laver polysaccharide aspect physico-chemical property, apparent pattern and the ntiviral characteristic, can substitute the raw material that the natural laver polysaccharide is used for suitability for industrialized production, be a kind of novel product polysaccharide resource.
Hair weeds cells liquid submerged fermentation method is produced Nostoc flagelliforme Polysaccharides, and with short production cycle, cost is low, does not need to consume wild hair-like seaweed resource, meets the policy of state guarantee hair-like seaweed resource ecology.At present, the research of producing Nostoc flagelliforme Polysaccharides for the hair weeds cells tank fermentation method mainly concentrates on the optimization of fermentation condition, such as the optimization of substratum, and the optimization of culture condition; Have no the pertinent literature report and utilize the control culture condition to improve Nostoc flagelliforme Polysaccharides output.
(3) summary of the invention
The present invention is in order to remedy the deficiencies in the prior art, provides a kind of and realizes easily, the method for the raising yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells of successful.
The present invention is achieved through the following technical solutions:
A kind of method that improves yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells take hair weeds cells as raw material, mainly comprises the steps:
(1) fs cultivated 4 days, adopt add glucose nitrogenous source BG-11 substratum arranged, intensity of illumination is controlled at 40～60 μ mol/m
2S;
(2) subordinate phase was cultivated 3 days, adopted and added the compound without nitrogenous source BG-11 substratum of glucose and sodium acetate, and intensity of illumination is controlled at 60～160 μ mol/m
2S.
In the step (1), the concentration of described glucose is 4g/L; In the step (2), described sodium acetate concentration is 2g/L, and glucose concn is 3～4g/L, medium pH 〉=9, and intensity of illumination is controlled at 100～160 μ mol/m
2S.
Hair weeds cells is ptotoautotroph, also can carry out mixotrophism and heterotrophic growth, intensity of illumination is the material impact factor in its process of growth, for Nostoc flagelliforme Polysaccharides, especially be secreted into the exocellular polysaccharide in the substratum, be the primary metabolite in the hair weeds cells process of growth, increase very fast in the later stage, therefore pass through control by stages intensity of illumination and medium component, the growth that improve hair weeds cells early stage, the later stage adopts the nitrogen-free agar of compounded carbons to carry out the high speed accumulation of Nostoc flagelliforme Polysaccharides.
Adopt Technology of the present invention, the hair weeds cells polysaccharide yield is significantly improved, its output increased to 3～4g/L.
Technique of the present invention is simple, and production efficiency is high, can significantly improve the output of hair weeds cells polysaccharide, is suitable for amplifying suitability for industrialized production, produces the hair weeds cells polysaccharide that obtains, and can be used as natural antiviral drug or food supplementary material after separation and purification.
(4) embodiment
Embodiment 1: comparative examples
Hair weeds cells kind of the present invention is that (it is tall long bright that Chinese patent ZL031191101.0, contriver: Jia Shiru Soviet Union build space, authorizes day: on March 8th, 2006) for the own separating and preserving of Tianjin industrial microorganism key lab.
This invention separates the hair weeds cells kind that obtains for a long time preservation from the wild frond of delivering vegetables, carry out the hair weeds cells suspension culture, adopt high-density culture technique, add organic carbon source the mixotrophism pattern (Chinese patent 2006100113589.4, it is forever virtuous that contriver: Jia Shiru builds the space woods in seapeak Soviet Union, open), adopt the BG-11 substratum that adds glucose, cultivated 24 ℃ of temperature 7 days, rotating speed 140rpm, intensity of illumination 40 ~ 90 μ mol/m
2S, Nostoc flagelliforme Polysaccharides output 2.25g/L.
Embodiment 2:
The illumination reaction device adopts control by stages intensity of illumination and culture method, and the conditions such as temperature, rotating speed are with comparative examples 1.
4 days of fs adopt in fermentation 4g/L glucose nitrogen BG-11 substratum arranged, intensity of illumination is controlled at 40 ~ 60 μ mol/m
2S, at the 5th day that cultivates, feed supplement 2g/L sodium-acetate and 3～3.5g/L glucose without nitrogen BG-11 substratum, regulate more than the pH9.0, the liquid level average light according to intensity control at 100～160 μ mol/m
2S.
Produce Nostoc flagelliforme Polysaccharides with this control by stages strategy, adopting ethanol precipitation to obtain polysaccharide yield is 3.5g/L.
Embodiment 3:
The illumination reaction device adopts control by stages intensity of illumination and culture method, and the conditions such as temperature, rotating speed are with comparative examples 1.
At the aerobic BG-11 substratum that adopted the glucose that adds 4g/L in 4 days of submerged fermentation fs, intensity of illumination is controlled at 40～60 μ mol/m
2S is cultivating the 5th day harvested cell, all change over to add 2g/L sodium-acetate and 4g/L glucose without in the nitrogen BG-11 substratum, regulate medium pH more than 9.0, intensity of illumination (liquid level mean value) is controlled at 100～160 μ mol/m
2S.
Produce Nostoc flagelliforme Polysaccharides with this control by stages strategy, it is 4g/L that two stage fermentation liquid adopt ethanol precipitation to obtain polysaccharide yield.
Claims (3)
1. a method that improves yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells take hair weeds cells as raw material, is characterized in that: mainly comprise the steps:
(1) fs cultivated 4 days, adopt add glucose nitrogenous source BG-11 substratum arranged, intensity of illumination is controlled at 40 ~ 60 μ mol/m
2S;
(2) subordinate phase was cultivated 3 days, adopted that to add concentration be the compound without nitrogenous source BG-11 substratum of the glucose of 3 ~ 4g/L and sodium acetate that concentration is 2g/L, medium pH 〉=9, and intensity of illumination is controlled at 60 ~ 160 μ mol/m
2S.
2. the method for raising yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells according to claim 1, it is characterized in that: in the step (1), the concentration of described glucose is 4g/L.
3. the method for raising yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells according to claim 1 and 2, it is characterized in that: in the step (2), described intensity of illumination is controlled at 100 ~ 160 μ mol/m
2S.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201010197762 CN101864470B (en) | 2010-06-11 | 2010-06-11 | Method for increasing yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells |
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| CN 201010197762 CN101864470B (en) | 2010-06-11 | 2010-06-11 | Method for increasing yield of extracellular polysaccharide produced by submerged fermentation of long thread moss cells |
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| CN101864470A CN101864470A (en) | 2010-10-20 |
| CN101864470B true CN101864470B (en) | 2013-01-09 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559799A (en) * | 2011-01-27 | 2012-07-11 | 河北工业大学 | Preparation method for algae endophytic fungi exocellular polysaccharide |
| CN102669520A (en) * | 2012-05-07 | 2012-09-19 | 天津科技大学 | Nostoc flagelliforme polysaccharide oral liquid and preparation method thereof |
| CN108531420A (en) * | 2018-03-19 | 2018-09-14 | 天津科技大学 | Method that is a kind of while improving hair weeds cells density and polysaccharide yield |
| CN113181222B (en) * | 2021-04-26 | 2022-04-01 | 武汉大学 | Application of extracellular polysaccharide metabolite of cryptococcus lactis in preparation of antiviral drugs |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1530439A (en) * | 2003-03-14 | 2004-09-22 | 天津科技大学 | Hair weeds bacterial culture and culturing product |
| CN101063085A (en) * | 2006-04-29 | 2007-10-31 | 天津科技大学 | Method for black moss cell high-density culture of stable high-yield black moss polysaccharide |
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2010
- 2010-06-11 CN CN 201010197762 patent/CN101864470B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1530439A (en) * | 2003-03-14 | 2004-09-22 | 天津科技大学 | Hair weeds bacterial culture and culturing product |
| CN101063085A (en) * | 2006-04-29 | 2007-10-31 | 天津科技大学 | Method for black moss cell high-density culture of stable high-yield black moss polysaccharide |
Non-Patent Citations (3)
| Title |
|---|
| Haifeng Yu et al.Growth characteristics of the cyanobacterium Nostoc flagelliforme in photoautotrophic, mixotrophic and heterotrophic cultivation.《J Appl Phycol》.2008,第21卷127-133. * |
| HaifengYuetal.GrowthcharacteristicsofthecyanobacteriumNostocflagelliformeinphotoautotrophic mixotrophic and heterotrophic cultivation.《J Appl Phycol》.2008 |
| Jianyu Su et al.Morphology, cell growth, and polysaccharide production of Nostoc flagelliforme in liquid suspension culture at different agitation rates.《J Appl Phycol》.2007,第20卷213－217. * |
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Granted publication date: 20130109 Termination date: 20130611 |