Early-stage rainwater microbial treatment method and device with the coupling of rainwater storage pond
Technical field.
The present invention relates to a kind of and the early-stage rainwater microbial treatment method storage pond coupling, belong to municipal drainage and environmental treatment system engineering technology field
Early-stage rainwater is in rainfall, owing to, contain a large amount of pollutents in the initial stage runoff that rainfall is formed, after the initial stage rainwater directly discharges water body to washing away of atmosphere, buildings, road surface etc., the receiving water body water quality that can work the mischief easily causes receiving water body black smelly phenomenon to occur.Therefore, early-stage rainwater being taked control techniques is the requisite measure of controlling city surface source pollution, preventing the receiving water body water quality deterioration.
Rainwater pollution control techniques aspect mainly adopts the storage pond technology at present abroad in the early stage, and the early-stage rainwater that municipal sewage system is collected is stored in earlier in the storage pond, with the impact of buffering early-stage rainwater pollutent to receiving water body.The rainwater that storage pond stores after storing after a while, discharging again after directly discharging in the water body or delivering to municipal wastewater treatment plant and handle by pipeline.The rainwater storage pond is that nearly 20 years developed countries are used for one of effective measure of controlling pollution of area source and rain-water drainage contradiction, except alleviating the impact pollution load of early-stage rainwater to receiving water body, the obstruction that can also cut down rainy day peak flow, elimination and minimizing drainage pipeline.Pollution problem is more and more paid attention to early-stage rainwater at home, also has many Rainwater Storage Tank to build or build.But, the storage pond of building at present both at home and abroad is that angle from the early-stage rainwater of regulating and storing designs and builds basically.
Throw the bacterium method and also be called as biological enhancement techniques or biological reinforcing technology.This is that a kind of handle has the single culture of specific purification function or the mixed strains of being made up of multiple bacterium, add in pollutant disposal system or the contaminated natural water by certain requirement, have the flora of the efficient purification function of pollutent or improve the density of certain class flora with structure, thereby can improve the biological treatment efficient of pollutant disposal system or promote the method for the self-purification capacity of water ecosystem.The distinguishing feature of throwing the bacterium method is to use conveniently, can add the place that needs use as required at any time to, and not complicated adding equipment, thereby obtains application more and more widely.But, throwing the bacterium method is to utilize microorganism that pollutent is purified, and the bacterial classification of input is from adapting to water quality, beginning to breed the needs regular hour in a large number then.
Storage pond provides temporal guarantee for the breeding of bacterial strain and bacterial strain to the degraded of pollutent, and storage pond is the space of a sealing simultaneously, can add the loss of bacterial classification for avoiding and the preservation of the bacterial classification that added provides advantageous conditions.
Summary of the invention
The objective of the invention is according to a kind of and early-stage rainwater microbial treatment method and device thereof the storage pond coupling.
A kind of early-stage rainwater microbial treatment method with the coupling of rainwater storage pond of the present invention is characterized in that it being to have following process and step:
The preparation of a, mixed bacterium suspension
At first with 11 kinds of bacterial classifications respectively under substratum and culture condition separately, enlarged culturing in the constant-temperature shaking culture case is carried out centrifugation with the high speed refrigerated centrifuge separator then; Physiological saline with an amount of 0.9% is mixed with bacteria suspension, and its concentration is adjusted to OD
400(optical density(OD)) value is 0.7; The bacteria suspension of 11 kinds of same concentrations is mixed by 1: 1 volume ratio, be mixed with the mixed bacterium suspension; Be put in the refrigerator below 4 ℃ and preserve, stand-by; The bacterial classification kind is in described 11: (1) proteus vulgaris (Proteus vulgaris), (2) pseudomonas mendocina (Pseudomonas mendocina), (3) greenery pseudomonas (Pseudomonas chlororaphis), (4) micrococcus luteus (Micrococcus luteus), (5) Pseudomonas fluorescens (Pseudomonas Fluorescens), (6) subtilis (Bacillus subtilis) (7) candida tropicalis (Candida tropicalis), (8) Candida boidinii (Candida boidinii), (9) coral Nocardia bacteria (Nocardia corallina), (10) bacillus pumilus (Bacillus pumilus), (11) Rhodopseudomonas palustris (Rhodopseudomonas palustris).
B, the above-mentioned mixed bacterium suspension for preparing is moved in throwing the bacterium case, valve and the storage pond rainwater water inlet pipe valve thrown on the bacterium case drain pipe link; Open in the time of by valve, described mixed bacterium suspension is added in the rainwater in the storage pond; The mixed bacterium suspension go out flow quantity be ten thousand of rainwater flooding velocity/;
C, meanwhile opens the aerating apparatus of being located at the storage pond bottom, under aeration condition, rainwater is carried out aerobic microbiological handle; Aeration mode is an intermittent aeration;
D, treat certain hour aeration and microbiological treatment after, the particle in microorganism flco and the early-stage rainwater is by at the bottom of leaving standstill and be deposited to the storage pond pond, treated good top clear water then drains in the natural water by the storage pond rising pipe.
2, the used dedicated system device of early-stage rainwater the microbial treatment method a kind of and coupling of rainwater storage pond, this system and device includes: rainwater storage pond, bacteria suspension are thrown bacterium case, rainwater water inlet pipe, are thrown tube, rising pipe, aeration tube, throwing tube valve and rainwater inlet valve; It is characterized in that: rainwater storage pond one side top is provided with a bacteria suspension and throws the bacterium case, and bacteria suspension is thrown the bacterium lower box part and is provided with the throwing tube, and a throwing tube valve is housed thereon; Bacteria suspension is thrown the bacterium case and is connected with the top of described rainwater storage pond by described throwing tube; One side also is provided with the rainwater water inlet pipe on rainwater storage pond top, and a rainwater inlet valve is housed thereon; The throwing tube intersects vertically with the rainwater water inlet pipe by pipe fitting and is connected; Be provided with the aeration tube and the regulated valve that can rouse air in the bottom of rainwater storage pond; Opposite side bottom at the rainwater storage pond is provided with the rising pipe that the early-stage rainwater that will handle is disposed to natural water; Being arranged on valve and the valve on the rainwater water inlet pipe thrown on the tube is to realize interlock by ad hoc linkage system, and promptly both realize opening synchronously or close synchronously; Described bacteria suspension is thrown bacterium case internal memory and is placed with 11 kinds of bacterial strain suspensions by 1: 1 formulated mixed bacterium suspension of volume ratio.
Characteristics of the present invention are as described below: characteristics of the present invention are with throwing bacterium method and the coupling of rainwater storage pond.On the one hand, the water storage function that the rainwater storage pond has can satisfy microorganism growth and breeding to the requirement of time, also can provide a sealing the space, avoid the loss of the bacterial classification that adds, thereby provide favourable envrionment conditions for the application of throwing the bacterium method; On the other hand, throw the bacterium method and have advantages such as easy to use, can the structure of existing rainwater storage pond not carried out under the prerequisite of bigger transformation, make the function of rainwater storage pond expand water quality purification function to from simple water storage function.
Therefore early-stage rainwater carries some microorganisms, have certain self-purification capacity, but this self-purification capacity is very low, and the present invention adds aerating apparatus, can increase to some extent the degradation effect of pollutent.By add the method for mixed bacterium suspension to storage pond, increase the microbe population in the early-stage rainwater, adjust the microbiology class group structure, can reach good early-stage rainwater decontamination effect improving.
The present invention has following advantage:
(1) technology is simple, and is easy to operate;
(2) need not newly-built treatment facility, processing costs is low;
(3) treatment time weak point is to COD, NH in the early-stage rainwater
3The removal effect of-N, TP, TN is good;
(4) environmentally safe reliable, do not produce secondary pollution.
Description of drawings
Fig. 1 for the using appts among the present invention promptly with the rough schematic of the rainwater microbe treatment system device of storage pond coupling.
Embodiment:
Now specific embodiments of the invention are described in this.
Embodiment: utilized above-mentioned dedicated system device to carry out the initial stage microbiological treatment of rainwater in the present embodiment.Technological process and the step handled are as follows:
1, at first with proteus vulgaris (Proteus vulgaris), pseudomonas mendocina (Pseudomonas mendocina), greenery pseudomonas (Pseudomonas chlororaphis), micrococcus luteus (Micrococcus luteus), Pseudomonas fluorescens (Pseudomonas Fluorescens), subtilis (Bacillus subtilis), bacillus pumilus (Bacillus pumilus), candida tropicalis (Candida tropicalis), Candida boidinii (Candida boidinii), coral Nocardia bacteria (Nocardia corallina), Rhodopseudomonas palustris (Rhodopseudomonas palustris) is under substratum and culture condition separately, fluid enlargement culture 24h in the constant-temperature shaking culture case, centrifugation then, remove supernatant liquor, physiological saline with an amount of 0.9% is mixed with bacteria suspension, and its concentration is adjusted to OD
400Value is 0.7.The bacteria suspension of 11 kinds of same concentrations is mixed with the mixed bacterium suspension by 1: 1 volume ratio, and the refrigerator of putting into below 4 ℃ is preserved stand-by.
2, the above-mentioned mixed bacterium suspension for preparing is put in throws in the bacterium case, valve and the storage pond rainwater water inlet pipe valve thrown on the bacterium case drain pipe link, and unlatching in the time of by valve is added to described mixed bacterium suspension in the rainwater in the storage pond; Mixed bacterium suspension flow be ten thousand of rainwater flooding velocity/.
3, meanwhile, open the aerating apparatus of being located at the storage pond bottom, under aeration condition, rainwater is carried out aerobic microbiological handle; Aeration mode is an intermittent aeration.The intermittent aeration cycle is every 2 hours aerations 4 hours, recirculation.
4, through aeration and microbiological treatment after 24 hours, the particle in microorganism flco and the early-stage rainwater is by at the bottom of leaving standstill and be deposited to the storage pond pond, and treated good top clear water then enters in the natural water by the storage pond rising pipe.
After above-mentioned processing, can make COD in the early-stage rainwater
CrClearance reach more than 70% NH
3The clearance of-N reaches more than 60%.
Used rainwater microbe treatment system device as shown in Figure 1 in the present embodiment.Fig. 1 is the rough schematic of native system device.
This system and device includes: rainwater storage pond 1, bacteria suspension are thrown bacterium case 2, rainwater water inlet pipe 3, throwing tube 4, rising pipe 5, aeration tube 6, are thrown tube valve 7 and rainwater inlet valve 8; Rainwater storage pond 1 one sides top is provided with a bacteria suspension and throws bacterium case 2 above rainwater storage pond 1 one sides, and bacteria suspension is thrown bacterium case 2 bottoms and is provided with throwing tube 4, and a throwing tube valve 7 is housed thereon; Bacteria suspension is thrown bacterium case 2 and is connected with the top of described rainwater storage pond 1 by described throwing tube 4; One side also is provided with rainwater water inlet pipe 3 on rainwater storage pond 1 top, and a rainwater inlet valve 5 is housed thereon; Throwing tube 4 intersects vertically with rainwater water inlet pipe 3 by pipe fitting and is connected; Be provided with the aeration tube 6 and the regulated valve that can rouse air in the bottom of rainwater storage pond 1; Be provided with the rising pipe 5 that the early-stage rainwater that will handle is disposed to natural water in the opposite side bottom of rainwater storage pond 1; Being arranged on valve 7 and the valve 8 on the rainwater water inlet pipe 3 thrown on the tube 4 is to realize interlock by ad hoc linkage system, and promptly both realize opening synchronously or close synchronously; Described bacteria suspension is thrown bacterium case 2 internal memories and is placed with 11 kinds of bacterial strain suspensions by 1: 1 formulated mixed bacterium suspension of volume ratio.
In the embodiment of the invention in used 11 bacterial classification available from Shanghai industrial microorganism institute, Chinese industrial microbial strains preservation center and China Committee for Culture Collection of Microorganisms common micro-organisms center.