CN101856320A - Preparation method of agrocybe aegerita fermentation whitening lotion - Google Patents
Preparation method of agrocybe aegerita fermentation whitening lotion Download PDFInfo
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- CN101856320A CN101856320A CN201010195443A CN201010195443A CN101856320A CN 101856320 A CN101856320 A CN 101856320A CN 201010195443 A CN201010195443 A CN 201010195443A CN 201010195443 A CN201010195443 A CN 201010195443A CN 101856320 A CN101856320 A CN 101856320A
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Abstract
The invention belongs to the field of bioengineering technology application, and in particular relates to a preparation method of agrocybe aegerita fermentation whitening lotion. The tea tree mushroom fermentation lotion is yellow liquid and is prepared by concentrating, deproteinizing and dialyzing liquid obtained from the submerged fermentation of the agrocybe aegerita. In the fermentation liquid, amino acid comprises the components of more than 18 percent of glutamic acid, more than 12 percent of aspartic acid, more than 8 percent of glycine, more than 7 percent of lysine and more than 6 percent of alanine, and besides, VC kojic acid ester and hyaluronic acid are also contained. The invention has whitening activity and can be applied to the preparation of cosmetics with the whitening effect.
Description
Technical field
The present invention relates to the preparation method of agrocybe aegerita fermentation whitening lotion.Belong to the biotechnology application.
Background technology
Agrocyb eaegerita belongs to Basidiomycetes, excrement rust umbrella section, and the field mushroom belongs to, and has another name called tea mushroom, oil tea mushroom, refreshing mushroom.Be a kind of edible fungi new varieties that development in recent years is got up.Agrocyb eaegerita is nutritious, and the protein content height contains the aminoacid of needed by human, and mineral elements such as abundant vitamin B group and potassium, sodium, calcium, magnesium, ferrum, zinc are arranged.The traditional Chinese medical science thinks to have higher medical value by flat, the Gan Wen, nontoxic of this mushroom property, heat clearing away, suppressing the hyperactive liver are arranged, makes eye bright, the effect of spleen invigorating.It is a kind of pollution-free food that integrates nutrition, health care, physical therapy.The fermentation liquid of adding other components acquisition Pear Power effects by submerged fermentation acquisition fermentation liquid is a kind of natural, and the composition of the Skin whitening care cosmetics of being free from side effects does not also have product now on the market.
Summary of the invention
The preparation method that the purpose of this invention is to provide a kind of agrocybe aegerita fermentation whitening lotion.
Technical scheme of the present invention is: the agrocyb eaegerita sporophore is inserted on the solid slant culture base by tissue isolation cultivate, treat to move to after length well and cultivate in the shake-flask seed liquid culture medium, cultured seed liquid is inserted the shake flask fermentation fluid medium cultivate.After the fermentation ends, fermentation liquid is concentrated, deproteinization obtains clarifying yellow liquid after the dialysis.Add other components, obtaining the liquid of Pear Power effect.
Specifically, we utilize commercially available agrocyb eaegerita to grow mycelium through tissue isolation on flat board.Mycelium access triangular flask fermentation back is centrifugal, and supernatant is through concentrating, and deproteinization obtains required fermentation liquid after the dialysis.Fermentation liquid 50%-60% by weight percentage; Add VC kojic acid fat 5% again; Hyaluronic acid 0.1%; Distilled water 44.9%-34.9%.
In the said process, slant medium is the PDA culture medium, the shake-flask seed culture medium is that PDA1L adds biphosphate potassium 1g, magnesium sulfate 0.5g, Carnis Bovis seu Bubali cream 2g, peptone 1g, pH6.0, fermentation medium are Semen Maydis powder 2g/100mL, yeast powder 0.3g/100mL, potassium dihydrogen phosphate 0.2g/100mL, magnesium sulfate 0.05g/100mL, and condition of culture is initial pH6.0, the bottled culture medium 100mL of 250mL triangle is in 25 ℃, 150r/min, shaken cultivation 7d, centrifugal back supernatant is removed free protein with the Sevage method, distill water dialysis is removed inorganic salt, obtains agrocyb eaegerita fermentation liquid.Get 50-60g after concentrating, add VC kojic acid fat 5g; Hyaluronic acid 0.1g; Distilled water is supplied 100mL.Entkeimung.
The specific embodiment
Following example will specify operational approach of the present invention, but can not be as limitation of the invention.
Example one
1. fermentation liquid preparation
The strain that sets out is commercially available agrocyb eaegerita (Agrocybe aegirit);
(1) tissue isolation
Slant medium: the PDA culture medium contains 1.5%～2.0% agar, the pH nature;
Method for tissue separation: after the sporophore appearance carried out surface sterilization with ethanol, put on the superclean bench, cut on stem and the cap intersection piece of tissue access slant medium with blade of sterilizing and tweezers, the pH nature, place under 25 ℃ of temperature and secretly cultivate, mycelia begins to sprout behind the 5d, checks 1 time every 2d, remove and depollute, select the test tube that mycelial growth rate is fast and growing way is strong to plant as pure mother;
(2) shake-flask seed is cultivated
Shake-flask seed culture medium: PDA1L adds biphosphate potassium 1g, magnesium sulfate 0.5g, Carnis Bovis seu Bubali cream 2g, peptone 1g, pH6.0;
Cultural method: get the identical truffle of 3～4 Semen Glyciness size from slant strains, be inoculated in the shake-flask seed culture medium, the bottled culture medium 100mL of 250mL triangle is in 25 ℃, 150r/min, shaken cultivation 7d;
(3) shake flask fermentation is cultivated
Fermentation medium: Semen Maydis powder 2g/100mL, yeast powder 0.3g/100mL, potassium dihydrogen phosphate 0.2g/100mL, magnesium sulfate 0.05g/100mL;
Cultural method: cultured seed is inserted in the shake flask fermentation culture medium 25 ℃ with 15% inoculum concentration, 150r/min, shaken cultivation 7d;
(4) fermentation liquid concentrates, deproteinization, dialysis
It is centrifugal to cultivate the back, gets and removes floating preteins with the Sevage method after the supernatant Rotary Evaporators concentrates, and the volume of promptly pressing fermentation liquid 1/5 adds chloroform, adds the n-butyl alcohol of chloroform volume 1/5 then, vibration 20min after mixing.Protein and chloroform-n-butyl alcohol generate gel, and the centrifugal 20min of 4000rpm collects supernatant, repeatable operation 8 times to chloroform-n-butanol layer not muddy till.Get then in the bag filter of packing in the supernatant, tighten bag mouth, be suspended in the distilled water, dialysis 48h, water was changed once every 12 hours in the centre, got fermentation liquid.
2. the interpolation of other components and Entkeimung
(1) interpolation of other components
Fermentation liquid is got 50-60g after concentrating, and adds VC kojic acid fat 5g; Hyaluronic acid 0.1g; Distilled water is supplied 100mL.
(2) Entkeimung
Aforesaid liquid is by the filter membrane degerming of 0.22 μ m.
Example two
The agrocyb eaegerita fermentation liquid sample has been carried out the aminoacid composition, the mensuration that monosaccharide is formed, measurement result shows, glutamic acid accounts for more than 18% in amino acid contained, and aspartic acid accounts for more than 12%, and glycine accounts for more than 8%, lysine accounts for more than 7%, alanine accounts for more than 6%, and contained monosaccharide is based on glucose, and recording polyoses content with the phenolsulfuric acid method is 76.3%.The aminoacid of agrocyb eaegerita fermentation liquid is formed and content sees Table 1.
The aminoacid of table 1 agrocyb eaegerita fermentation liquid is formed and content
Claims (5)
1. the agrocyb eaegerita fermentation liquid of a Pear Power effect is characterized in that outward appearance is a weak yellow liquid, is that agrocyb eaegerita concentrates by the fermentation liquid process that submerged fermentation obtains, deproteinization, add VC kojic acid fat after the dialysis again, hyaluronic acid and distilled water make after the Entkeimung.Wherein the amino acid contained middle glutamic acid of fermentation liquid accounts for more than 18%, and aspartic acid accounts for more than 12%, and glycine accounts for more than 8%, and lysine accounts for more than 7%, and alanine accounts for more than 6%.
2. the preparation method of the described whitening lotion of claim 1, this method is made up of the following step:
(1) spawn culture
The strain that sets out is commercially available agrocyb eaegerita (Agrocybe aegirit);
1. tissue isolation
Slant medium: the PDA culture medium contains 1.5%～2.0% agar, the pH nature;
Method for tissue separation: after the sporophore appearance carried out surface sterilization with ethanol, put on the superclean bench, cut on stem and the cap intersection piece of tissue access slant medium with blade of sterilizing and tweezers, the pH nature, place under 25 ℃ of temperature and secretly cultivate, mycelia begins to sprout behind the 5d, checks 1 time every 2d, remove and depollute, select the test tube that mycelial growth rate is fast and growing way is strong to plant as pure mother;
2. shake-flask seed is cultivated
Shake-flask seed culture medium: PDA1L adds biphosphate potassium 1g, magnesium sulfate 0.5g, Carnis Bovis seu Bubali cream 2g, peptone 1g, pH6.0;
Cultural method: get the identical truffle of 3～4 Semen Glyciness size from slant strains, be inoculated in the shake-flask seed culture medium, the bottled culture medium 100mL of 250mL triangle is in 25 ℃, 150r/min, shaken cultivation 7d;
3. shake flask fermentation is cultivated
Fermentation medium: Semen Maydis powder 2g/100mL, yeast powder 0.3g/100mL, potassium dihydrogen phosphate 0.2g/100mL, magnesium sulfate 0.05g/100mL;
Cultural method: cultured seed is inserted in the shake flask fermentation culture medium 25 ℃ with 15% inoculum concentration, 150r/min, shaken cultivation 7d;
(2) fermentation liquid concentrates, deproteinization, dialysis
It is centrifugal to cultivate the back, gets and removes floating preteins with the Sevage method after the supernatant Rotary Evaporators concentrates, and gets the supernatant dialysis then;
(3) interpolation of other components and Entkeimung
1. the interpolation of other components
Fermentation liquid concentrates the back and adds VC kojic acid fat; Hyaluronic acid; Distilled water;
2. Entkeimung
Aforesaid liquid is by the filter membrane degerming of 0.22 μ m.
3. the preparation method of whitening lotion as claimed in claim 2 is characterized in that the described Sevage of using method removal floating preteins, is the volume adding chloroform by fermentation liquid 1/5, adds the n-butyl alcohol of chloroform volume 1/5 then, and 20min vibrates after mixing.Protein and chloroform-n-butyl alcohol generate gel, and the centrifugal 20min of 4000rpm collects supernatant, repeatable operation 8 times to chloroform-n-butanol layer not muddy till.
4. the preparation method of whitening lotion as claimed in claim 2 is characterized in that described dialysis is meant the fermentation liquid of removing free protein is packed in the bag filter, tightens bag mouth, is suspended in the distilled water, and dialysis 48h middlely changed water once every 12 hours.
5. the preparation method of whitening lotion as claimed in claim 2 is characterized in that being added to of described other components get the fermentation liquid 50-60g after concentrating, and adds VC kojic acid fat 5g; Hyaluronic acid 0.1g; Distilled water is supplied 100mL.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178702A (en) * | 2011-05-06 | 2011-09-14 | 厦门大学 | Tea tree mushroom extract and preparation method and application of extract |
| CN102429855A (en) * | 2011-12-15 | 2012-05-02 | 江南大学 | Preparation method of agrocybe cylindracea fruit body extracting solution with whitening efficacy |
| CN103655435A (en) * | 2013-04-28 | 2014-03-26 | 钱臻 | Biologically fermented hair conditioner and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005089390A (en) * | 2003-09-19 | 2005-04-07 | Kanebo Cosmetics Inc | Cosmetic |
| JP2008266173A (en) * | 2007-04-18 | 2008-11-06 | Idemitsu Kosan Co Ltd | Hair growth promoting composition |
| WO2010050369A1 (en) * | 2008-10-31 | 2010-05-06 | 国立大学法人岡山大学 | Prophylactic agent for dental caries |
-
2010
- 2010-06-09 CN CN2010101954432A patent/CN101856320B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005089390A (en) * | 2003-09-19 | 2005-04-07 | Kanebo Cosmetics Inc | Cosmetic |
| JP2008266173A (en) * | 2007-04-18 | 2008-11-06 | Idemitsu Kosan Co Ltd | Hair growth promoting composition |
| WO2010050369A1 (en) * | 2008-10-31 | 2010-05-06 | 国立大学法人岡山大学 | Prophylactic agent for dental caries |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178702A (en) * | 2011-05-06 | 2011-09-14 | 厦门大学 | Tea tree mushroom extract and preparation method and application of extract |
| CN102178702B (en) * | 2011-05-06 | 2013-01-02 | 厦门大学 | Tea tree mushroom extract and preparation method and application of extract |
| CN102429855A (en) * | 2011-12-15 | 2012-05-02 | 江南大学 | Preparation method of agrocybe cylindracea fruit body extracting solution with whitening efficacy |
| CN103655435A (en) * | 2013-04-28 | 2014-03-26 | 钱臻 | Biologically fermented hair conditioner and preparation method thereof |
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| CN101856320B (en) | 2012-11-21 |
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