CN101851594A - Liquid fermentation culturing method of bacillus strain - Google Patents
Liquid fermentation culturing method of bacillus strain Download PDFInfo
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- CN101851594A CN101851594A CN 201010120296 CN201010120296A CN101851594A CN 101851594 A CN101851594 A CN 101851594A CN 201010120296 CN201010120296 CN 201010120296 CN 201010120296 A CN201010120296 A CN 201010120296A CN 101851594 A CN101851594 A CN 101851594A
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- bacillus strain
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Abstract
The invention relates to a liquid fermentation culturing method of bacillus strain, which comprises the step of: inoculating seed bacterial liquid of the bacillus strain into a liquid fermentation tank filled with culture medium for cultivation, wherein the inoculation cell age of the seed bacterial liquid is 8-12h, the inoculation quantity is 3%, the culture medium is YSP culture medium, the initial PH valve before inoculation is 7.0-7.5, the temperature in the tank when in cultivation of the fermentation tank is 30-36 DEG C, the rotating speed of the fermentation tank is 120r/min when in cultivation, and the incubation time is 16-30h. The invention researches the factors such as the culture medium type, the initial PH valve of culture solution, the cultivation temperature, the cultivation time, the inoculation quantity and the like which influence the liquid fermentation of the bacillus strain, so that the optimal cultivation condition of the strain can be obtained. The yield of the bacillus strain cultured by the culturing method is more than that of the bacillus strain cultured by the conventional method, and the fermentation cost is low.
Description
Technical field
The present invention relates to a kind of fermentation culture method of biological bacterial strain, specifically the liquid fermentation culturing method of Bacillus strain.
Background technology
Subtilis is to have a liking for the aerobic sporiferous rod-shaped bacterium of warm nature, is widespread in nature, and is nontoxic to people and animals, can produce multiple antibiotic and enzyme, has broad spectrum antibiotic activity.Existing a large amount of bibliographical information liquid culture condition can influence the increment of subtilis, but there are differences aspect the suitableeest incubation time, culture temperature and the substratum initial p H value, showing that there is significant difference in the different strains that belongs to biocontrol microorganisms of the same race together aspect fermentation culture conditions.
Bacillus strain is to separate a strain biocontrol microorganisms that obtains from soil, laboratory experiment shows that the thalline of Bacillus strain and non-volatile meta-bolites all have bacteriostatic action to sclerotinia rot of colza, dry thread Pyrenomycetes, botrytis cinerea, trifolium southern blight, have the wide development application prospect.At present conventional cultural method is all adopted in the cultivation of Bacillus strain, the optimization cultural method that how to obtain Bacillus strain does not also carry out deep research.
Summary of the invention
The object of the present invention is to provide a kind of liquid fermentation culturing method of Bacillus strain of optimization,, reduce fermentation costs to obtain the maximum growth amount of Bacillus strain.
The present invention is achieved in that
A kind of liquid fermentation culturing method of Bacillus strain, be the kind daughter bacteria liquid of Bacillus strain to be seeded in interior a cultivation of liquid fermentation tank that fills substratum form, it is characterized in that: the inoculation cell age of described kind of daughter bacteria liquid is 8～12h, inoculum size is 3%, described substratum is the YSP substratum, the initial p H value of substratum before inoculation is 7.0～7.5, temperature is 30～36 ℃ in the jar of described fermentor tank when cultivating, to place rotating speed be on the shaking table of 120r/min to fermentor tank during cultivation, and incubation time is 16～30h.
Further scheme is: the volume of described substratum and the volumetric ratio of fermentor tank are 1/5.
Further scheme is: the prescription of described substratum is sal epsom 0.056g/L, ferrous sulfate 0.057g/L, lime carbonate 1.631g/L, analysis for soybean powder 19.847g/L, yeast soaks powder 3g/L, manganous sulfate 0.1g/L, dipotassium hydrogen phosphate 2g/L, sucrose 10g/L, Semen Maydis powder 20g/L, surplus is a water.
Cultivate Bacillus strain by cultural method of the present invention, can obtain the output of more Bacillus strain than ordinary method, relative fermentation costs is low.
Embodiment
Embodiment one
A kind of liquid fermentation culturing method of Bacillus strain, be the kind daughter bacteria liquid of the Bacillus strain of cell age 10h to be seeded in the liquid fermentation tank that fills the YSP substratum with 3% inoculum size cultivate, the pH value of substratum before inoculation is 7.2, temperature is 33 ℃ in the fermentor tank, it is on the shaking table of 120r/min that fermentor tank places rotating speed, cultivates 20h, and wherein the volumetric ratio of the volume of substratum and fermentor tank is 1/5, the prescription of substratum is sal epsom 0.056g/L, ferrous sulfate 0.057g/L, lime carbonate 1.631g/L, analysis for soybean powder 19.847g/L, yeast soaks powder 3g/L, manganous sulfate 0.1g/L, dipotassium hydrogen phosphate 2g/L, sucrose 10g/L, Semen Maydis powder 20g/L, surplus is a water.
Embodiment two
Cell age was 8h when the kind daughter bacteria liquid of Bacillus strain was inoculated, and the pH value of substratum before inoculation is 7.5, and temperature is 36 ℃ in the fermentation tube, and incubation time is 16h, and all the other are with embodiment one.
Embodiment three
Cell age was 12h when the kind daughter bacteria liquid of Bacillus strain was inoculated, and the pH value of substratum before inoculation is 7.0, and temperature is 30 ℃ in the fermentation tube, and incubation time is 30h, and all the other are with embodiment one.
The increment average out to 1.55 * 10 of the Bacillus strain that the foregoing description is cultivated
8Cfu/ml, the increment of the Bacillus strain of cultivating than the cultural method of routine has improved 43.14%.
Claims (3)
1. the liquid fermentation culturing method of a Bacillus strain, be the kind daughter bacteria liquid of Bacillus strain to be seeded in interior a cultivation of liquid fermentation tank that fills substratum form, it is characterized in that: the inoculation cell age of described kind of daughter bacteria liquid is 8～12h, inoculum size is 3%, described substratum is the YSP substratum, the initial p H value of substratum before inoculation is 7.0～7.5, temperature is 30～36 ℃ in the jar of described fermentor tank when cultivating, to place rotating speed be on the shaking table of 120r/min to fermentor tank during cultivation, and incubation time is 16～30h.
2. the liquid fermentation culturing method of Bacillus strain according to claim 1, it is characterized in that: the volume of described substratum and the volumetric ratio of fermentor tank are 1/5.
3. the liquid fermentation culturing method of Bacillus strain according to claim 1 and 2, it is characterized in that: the prescription of described substratum is sal epsom 0.056g/L, ferrous sulfate 0.057g/L, lime carbonate 1.631g/L, analysis for soybean powder 19.847g/L, yeast soaks powder 3g/L, manganous sulfate 0.1g/L, dipotassium hydrogen phosphate 2g/L, sucrose 10g/L, Semen Maydis powder 20g/L, surplus is a water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010120296 CN101851594A (en) | 2010-03-09 | 2010-03-09 | Liquid fermentation culturing method of bacillus strain |
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| CN 201010120296 CN101851594A (en) | 2010-03-09 | 2010-03-09 | Liquid fermentation culturing method of bacillus strain |
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| CN 201010120296 Pending CN101851594A (en) | 2010-03-09 | 2010-03-09 | Liquid fermentation culturing method of bacillus strain |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1590535A (en) * | 2004-03-27 | 2005-03-09 | 中国科学院等离子体物理研究所 | Bacillus subtilis strain, preparation method, preparation preparing proess and use |
| CN101412983A (en) * | 2008-11-20 | 2009-04-22 | 江苏省苏微微生物研究有限公司 | Bacillus coagulans, preparation of high-density cultivated spore preparation, and use thereof |
-
2010
- 2010-03-09 CN CN 201010120296 patent/CN101851594A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1590535A (en) * | 2004-03-27 | 2005-03-09 | 中国科学院等离子体物理研究所 | Bacillus subtilis strain, preparation method, preparation preparing proess and use |
| CN101412983A (en) * | 2008-11-20 | 2009-04-22 | 江苏省苏微微生物研究有限公司 | Bacillus coagulans, preparation of high-density cultivated spore preparation, and use thereof |
Non-Patent Citations (1)
| Title |
|---|
| 《中国优秀所示学位论文数据库》 20080229 刘琳 芽孢杆菌BS2004菌株的鉴定、抑菌作用及发酵配方优化研究 1-3 , 2 * |
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Open date: 20101006 |