CN101838669A - Method for producing bio-oil by using fish meal wastewater - Google Patents
Method for producing bio-oil by using fish meal wastewater Download PDFInfo
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- CN101838669A CN101838669A CN201010159271A CN201010159271A CN101838669A CN 101838669 A CN101838669 A CN 101838669A CN 201010159271 A CN201010159271 A CN 201010159271A CN 201010159271 A CN201010159271 A CN 201010159271A CN 101838669 A CN101838669 A CN 101838669A
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Abstract
The invention relates to a method for producing bio-oil by using fish meal wastewater and belongs to the field of wastewater resource utilization. The method is characterized in that the yeasts are promoted to accelerate multiplication by taking the fish meal wastewater as culture media, domesticating and amplifying oil yeasts; and adding a nutrition micro-adjusting agent for improving the micro-environment; and the bio-oil is prepared by the centrifugal-chloroform extracting technology. The method has the characteristics of simple operation, low investment, high culture speed of the yeasts and high fermentation efficiency.
Description
One, technical field:
The present invention relates to a kind of method of utilizing fish meal wastewater to produce bio-oil, belong to the utilization of wastewater resource field.
Two, technical background:
Industry such as starch, soybean protein waste water could prepare bio-oil because concentration is low as microzyme culture medium after needing to concentrate, so complex manufacturing, the running cost height.And the fish meal wastewater organic concentration is 5～10 times of waste strengths such as starch, satisfies the nutrient chemical requirement fully from trophic component.But such wastewater through organic matter kind complexity, saltiness are also high, are difficult to use as substratum.
Three, summary of the invention:
The present invention is directed to fish meal wastewater nutrition composition characteristics, having developed a kind of is substratum with the fish meal wastewater, utilizes yeast to prepare the novel process of bio-oil fast.This process using step-wise manner is cultivated, and by adding nutrition fine setting agent, rationally starts and quickens fermentation reaction.
The present invention utilizes fish meal wastewater to produce the method for bio-oil, and its step is as follows:
(1) domestication: raw wastewater at first is diluted to 1/4 concentration, and the oleaginous yeast bacterium that inoculation is selected for use is tamed saccharomycetic adaptability in fermentor tank;
(2) the amplification phase: raw wastewater is diluted to 1/2 concentration, and the yeast of cultivating in the inoculation step 1 is in fermentor tank, and yeast output increases;
(3) the microorganism variation that need shake down, through after the adaptive phase, biomass increases gradually, tends towards stability after 6 days in fermentation culture;
(4) production phase: the yeast in the step 2 is seeded in the waste water stoste, place each fermentor tank, this stage adds nutrition fine setting agent and improves micro, make yeast quicken propagation, fermentor tank maintenance temperature is 30 ℃ in the production, the rotating speed agitator speed is 200rpm, and every jar of culture cycle is 6 days;
(5) adopt centrifugal-chloroform extraction technology, above-mentioned fermenation raw liquid after centrifugal, got settling and added CHCl
3, CH
3The OH sonic oscillation behind the residue supernatant liquor volatilization chloroform, is promptly prepared bio-oil, the chloroform reusable edible.
Nutrition fine setting agent of the present invention is yeast extract paste 75-85%, ferrimanganic trace element 5-10%, sodium acetate buffer agent 15-25%.
The present invention has the following advantages: fish meal wastewater is directly as substratum, compares with other waste water to have simple to operately, invests low characteristics; Adopt the staged training method, yeast is tamed, cultivation speed is fast; The nutrition fine setting agent of exploitation can not only be regulated the nutrition ratio of waste water, and can adjust the pH of yeasting, so saccharomycetes to make fermentation efficient height, and the fish meal wastewater utilising efficiency is fast, and after the fermentation, surplus materials is few; Because surplus materials is few, and its water ratio is low, after adding nutritive substance a little and adjusting, can be used as feed ingredient.
Four, specific embodiment:
(1) domestication: raw wastewater at first is diluted to 1/4 concentration, and the oleaginous yeast bacterium that inoculation is selected for use is in each fermentor tank, and fermentor tank is that mechanical type stirs, and rotating speed is 200rpm, and temperature is 30 ℃.Culture cycle is to tame saccharomycetic adaptability in 6 days, uses in order to the next stage inoculation.
(2) the amplification phase: raw wastewater is diluted to 1/2 concentration, the yeast of cultivating in the inoculation step 1, in fermentor tank, keeping temperature is 30 ℃ with the bacterial classification inoculation cultivated, agitator speed is 200rpm.Culture cycle is 6 days.Amplification yeast output is used in order to inoculation in producing.
(3) step 1 and step 2 are not added any material for kind of a bacterium domestication, amplification phase.This stage, because the variation that microorganism need shake down, so biomass begins to have a tangible downtrending.Through after the adaptive phase, biomass increases gradually, and in the time of 6 days, biomass reaches maximum value 5.34g/L, tends towards stability afterwards in fermentation culture.Grease productive rate and fat content present and the similar changing conditions of biomass, and the two all when cultivating 6d, reaches maximum value 1.11g/L and 20.79% respectively.
(4) production phase: the yeast in the step 2 is seeded in the waste water stoste, places each fermentor tank, enter into the starting period and the acceleration period of production.This stage adds nutrition fine setting agent and improves micros such as carbon-nitrogen ratio and pH, makes yeast quicken propagation.This nutrition fine setting agent comprises 75% yeast extract paste, 5% ferrimanganic trace element and 20% sodium acetate buffer agent, quickens yeast propagation.The nutritive salt that adds with the culture volume ratio is: 1: 1000, once a day.Fermentor tank maintenance temperature is 30 ℃ in the production, and the rotating speed agitator speed is 200rpm.Every jar of culture cycle is 6 days.
(5) airtight negative pressure workshop is set, the bio-oil extracting mode adopts centrifugal-chloroform extraction technology, chloroform recycle.Get fermented liquid behind centrifugal 10min under the 3000r/min, 1: 2 (v/v) CHCl that gets settling
3: CH
3The OH sonic oscillation, 3000r/min is centrifugal then, behind the supernatant liquor volatilization chloroform, prepares bio-oil.
Claims (3)
1. method of utilizing fish meal wastewater to produce bio-oil is characterized in that the step of this method is as follows:
(1) domestication: raw wastewater at first is diluted to 1/4 concentration, and the oleaginous yeast bacterium that inoculation is selected for use is tamed saccharomycetic adaptability in fermentor tank;
(2) the amplification phase: raw wastewater is diluted to 1/2 concentration, and the yeast of cultivating in the inoculation step 1 is in fermentor tank, and yeast output increases;
(3) the microorganism variation that need shake down, through after the adaptive phase, biomass increases gradually, tends towards stability after 6 days in fermentation culture;
(4) production phase: the yeast in the step 2 is seeded in the waste water stoste, place each fermentor tank, this stage adds nutrition fine setting agent and improves micro, make yeast quicken propagation, fermentor tank maintenance temperature is 30 ℃ in the production, the rotating speed agitator speed is 200rpm, and every jar of culture cycle is 6 days;
(5) adopt centrifugal-chloroform extraction technology, above-mentioned fermenation raw liquid after centrifugal, got settling and added CHCl
3, CH
3The OH sonic oscillation behind the residue supernatant liquor volatilization chloroform, is promptly prepared bio-oil, the chloroform reusable edible.
2. a kind of method of utilizing fish meal wastewater to produce bio-oil according to claim 1 is characterized in that nutrition fine setting agent is yeast extract paste 75-85%, ferrimanganic trace element 5-10%, sodium acetate buffer agent 15-25%.
3. a kind of method of utilizing fish meal wastewater to produce bio-oil according to claim 1 is characterized in that institute adds CHCl
3, CH
3The volume ratio of OH is 1: 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010159271A CN101838669A (en) | 2010-04-29 | 2010-04-29 | Method for producing bio-oil by using fish meal wastewater |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010159271A CN101838669A (en) | 2010-04-29 | 2010-04-29 | Method for producing bio-oil by using fish meal wastewater |
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| Publication Number | Publication Date |
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| CN101838669A true CN101838669A (en) | 2010-09-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010159271A Pending CN101838669A (en) | 2010-04-29 | 2010-04-29 | Method for producing bio-oil by using fish meal wastewater |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103255185A (en) * | 2012-02-21 | 2013-08-21 | 华东理工大学 | Method for producing microbial oil through lignocellulose simultaneous saccharification and fermentation, and for recycling cellulase |
-
2010
- 2010-04-29 CN CN201010159271A patent/CN101838669A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103255185A (en) * | 2012-02-21 | 2013-08-21 | 华东理工大学 | Method for producing microbial oil through lignocellulose simultaneous saccharification and fermentation, and for recycling cellulase |
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Application publication date: 20100922 |