CN101837116B - Liver-protecting and alcoholism-relieving product using Saccharomyces cerevisiae as main material - Google Patents
Liver-protecting and alcoholism-relieving product using Saccharomyces cerevisiae as main material Download PDFInfo
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Abstract
The invention provides a liver-protecting and alcoholism-relieving product using Saccharomyces cerevisiae, which contains over 42 percent of proteins, more than 500 milligrams of total B vitamins per kilogram and more than 800 milligrams of glutathione per kilo gram, as main materials. The invention also provides a method for producing the liver-protecting and alcoholism-relieving product.
Description
Technical field
The present invention relates to a kind of liver-protecting and alcoholism-relieving product that saccharomyces cerevisiae (Saccharomyces cerevisiae) is primary raw material of take, be particularly related to a kind of with protein content > 42%, vitamin B group total content > 500mg/kg, the saccharomyces cerevisiae of glutathione content > 800mg/kg as the liver-protecting and alcoholism-relieving product of primary raw material.
Background technology
Characters of Chinese Alcoholic Drink Culture is with a long history, is exactly drinks consumption big country since ancient times, produce every year various wine and reach more than 9,000 ten thousand tons, and wine output is every year with 69% speed increment.According to authoritative statistics, the wine people of China are up to 500,000,000, and also have many people bearing commercial affairs dinner party, handing over friend to tie many worries that are unequal to capacity for liquor such as friend, society's tackling key problem.But long-term heavy drinking can cause alcoholic liver injury, finally can develop into even liver cirrhosis of chronic active hepatitis.Heavy drinking in the short-term a few days, also can cause lighter hepatic injury, as alcoholic fatty liver.Concerning having basic hepatopathy as the people of chronic hepatitis B, a very important risk factor of drinking and making often chronic hepatitis b disease feelings increase the weight of.At present according to the ASSOCIATE STATISTICS of medical department, the senile disease rejuvenations increasingly such as the hypertension of China, hyperlipidemia, and ascendant trend linearly, crucial reason is exactly insobriety.
Current relieving alcoholism and protecting the liver product mainly be take Chinese medicine ingredients as main: Chinese patent application CN02148397A has disclosed a kind of nutritious milk sugar product with sobering up and liver protecting functions, and it is mainly multivitamin, mineral and plurality of Chinese and forms; Chinese patent application CN200810006421A has disclosed a kind of health beverage of relieving alcoholism and protecting the liver, and main component is Mel, Lac regis apis, propolis and Fructus Crataegi.These take Chinese medicine ingredients as main relieving alcoholism and protecting the liver product, be foundation, but these Chinese medicine ingredients still may produce potential harm to human body, do not belong to the food of All Pure Nature mainly with traditional prescription.And these tcm products absorb slower, the liver protecting is avoided ethanol injury or is recovered from the injury of ethanol in time.
In view of current this present situation, the liver-protecting and alcoholism-relieving product of developing a kind of pure natural has very great significance to the mankind's healthy tool.In vitamin, and liver have compared with substantial connection be vitamin B group, drink and too much wait and cause liver injury, also parallel with Vitamin B deficiency disease under many occasions.These vitamin B group are push body intracellular metabolites, indispensable material when sugar, fat, protein etc. are changed into heat.Glutathion is a kind of amino acid whose polymer, glutamic acid, cysteine, glycine, consists of, and has very high nutritive value, is in human body metabolism, to manufacture the primary raw material of enzyme.Glutathion can promote the catabolism of liver equally, opposing chemical liver injury, and can repair impaired hepatocyte, safeguard hepatocellular normal function.In yeast, contain abundant vitamin B group and glutathion, so yeast can deeply nourish and the liver protecting, guarantee that liver detoxification function normally moves.In yeast, also contain in addition a lot of trace element, these trace element are important component parts of some enzymes.Therefore, there is at present people yeast to be applied in the medicine of hepatic disease and drug intoxication treatment, but in existing yeast liver-protecting and alcoholism-relieving product few in number, the content of vitamin B group and glutathion is not high and exist uncertain, thereby the homogeneity of product effective content is poor, can not reach good liver-protecting and alcoholism-relieving effect.
Summary of the invention
The object of the present invention is to provide a kind of All Pure Nature, efficient, novel, stablize, be easy to absorb take the relieving alcoholism and protecting the liver product that saccharomyces cerevisiae is primary raw material, the protein content > 42% of the saccharomyces cerevisiae wherein adopting, vitamin B group total content > 500mg/kg, glutathione content > 800mg/kg.
In yeast product provided by the invention; also can further add yeast itself rich; the nutrients such as glutathion, vitamin B group the liver protecting to certain effect, produce synergism with the proteins,vitamins,minerals of yeast itself, reach better relieving alcoholism and protecting the liver effect.
Provided by the inventionly take in the relieving alcoholism and protecting the liver product that saccharomyces cerevisiae is primary raw material, except adding glutathion, vitamin B group, can also add alternatively one or more batchings in the group of selecting free yeast rich in selenium, yeast rich in zinc, sugarcane sugar and starch composition.
Provided by the inventionly take in the relieving alcoholism and protecting the liver product that saccharomyces cerevisiae is primary raw material, every gram of product contains 0.10～0.55g protein, 10～200000 μ g thiamines, 10～200000 μ g riboflavin, 10～200000 μ g VB
6, 1～50000 μ g folic acid, 1～200000 μ g nicotinic acid, 10～1000000 μ g pantothenic acid and 1～50000 μ g glutathion.
Another object of the present invention is to provide a kind of method of preparing described relieving alcoholism and protecting the liver product, it comprises: 1) saccharomyces cerevisiae is mixed to the emulsion that it is 10%～30% that water is mixed with containing dry with batching; 2) described emulsion is stirred; 3) vapour pressure at heated roller is under 0.5～0.65MPa, described emulsion be dried to flakes in cylinder or pulverize as Powdered, the flakes obtaining or powdered substance are divided and installed in polyethylene bottle, and wherein said batching is selected one or more components in the group that free vitamin B group, glutathion, yeast rich in selenium, yeast rich in zinc, sugarcane sugar and starch form.
The present invention also provides a kind of method of preparing described relieving alcoholism and protecting the liver product, comprising: 1) saccharomyces cerevisiae and batching are pulverized in pulverizer, the powder obtaining is crossed to 80 mesh sieves; 2) fully mix described powder, and minute install in capsule, make capsule product, wherein said batching is selected one or more components in the group that free vitamin B group, glutathion, yeast rich in selenium, yeast rich in zinc, sugarcane sugar and starch form.
The present invention further provides a kind of method of preparing described relieving alcoholism and protecting the liver product, having comprised: 1) saccharomyces cerevisiae is mixed homogeneously and pulverized in pulverizer with batching, the powder obtaining has been crossed 100 mesh sieves; 2) water, as solvent, is made aaerosol solution by described powder; 3) by described suspension filled with solution in vial, autoclaving at 121 ℃, make oral liquid product, wherein said batching is selected one or more components in the group that free vitamin B group, glutathion, yeast rich in selenium, yeast rich in zinc, sugarcane sugar and starch form.
The method that the invention provides the described relieving alcoholism and protecting the liver product of another kind of preparation, comprising: 1) saccharomyces cerevisiae is mixed homogeneously with batching, and pulverize in pulverizer, the powder obtaining is crossed 100 mesh sieves; 2) adopt the soluble starch of 32% dextrin and 25% as excipient and dispersant, 75% alcoholic solution is as wetting agent, baking temperature is controlled at 55 ℃～65 ℃, described powder is granulated, make granule product, wherein said batching is selected one or more components in the group that free vitamin B group, glutathion, yeast rich in selenium, yeast rich in zinc, sugarcane sugar and starch form.
The method that the invention provides the described relieving alcoholism and protecting the liver product of another kind of preparation, comprising: 1) saccharomyces cerevisiae and batching are pulverized in pulverizer, the powder obtaining is crossed 100 mesh sieves; 2) by the abundant mix homogeneously of the powder obtaining; 3) directly powder is placed in tablet machine and carries out tabletting, make tablet product.Wherein said batching is selected one or more components in the group that free vitamin B group, glutathion, yeast rich in selenium, yeast rich in zinc, sugarcane sugar and starch form.
The specific embodiment
One, raw material
To purchase from the market available saccharomyces cerevisiae product adopts after national testing standard quantitative assay, filter out protein content > 42%, vitamin B group total content > 500mg/kg, the saccharomyces cerevisiae of glutathione content > 800mg/kg is as primary raw material.
Batching: glutathion, thiamine, riboflavin, nicotinic acid, folic acid, pantothenic acid, biotin, inositol, choline, VB
6, VB
12, yeast rich in selenium, yeast rich in zinc, sucrose, starch, above batching all can directly be bought acquisition from the market, various batching purity is all more than 99%, and is food grade products.
Two, the preparation of liver-protecting and alcoholism-relieving yeast product
Example 1
Material therefor: bakery yeast (belonging to saccharomyces cerevisiae belongs to), starch
Preparation process: the bakery yeast screening is mixed to the emulsion that it is 20% that water is mixed with containing dry with starch according to the mass ratio ratio of 80: 20; Fully stir and obtain uniform emulsion; Vapour pressure at heated roller is under 0.5～0.65MPa, emulsion is dried to flakes material in cylinder or pulverizes as Powdered; The flakes obtaining or powdered substance are divided and installed in polyethylene bottle.
Each step is all carried out in 300,000 grades of GMP (the pharmaceutical production specifications of quality) workshop above, to guarantee health and the safety of product.
Example 2
Material therefor: 20% bakery yeast, 5% glutathion, 10% thiamine, 10% riboflavin, 10%VB
6, 10% nicotiamide, 15% calcium pantothenate, 5% folic acid, 10% yeast rich in selenium (containing selenium 2000ppm) and 5% starch, above percentage composition is quality percentage composition.
Preparation process:
1) pulverize: the material of above-mentioned formula ratio is pulverized with pulverizer, then the powder obtaining is crossed to 80 mesh sieves;
2) mix: by the abundant mix homogeneously of the powder obtaining;
3) fill and packing: by mixed powder filling capsule, every 0.1～0.5g;
Each step is all carried out in 300,000 grades of GMP workshops, to guarantee health and the safety of product.
Example 3
Material therefor: 30% beer yeast (belonging to saccharomyces cerevisiae belongs to), 1% glutathion, 20% thiamine, 4% riboflavin, 2%VB
6, 3% nicotiamide, 28% calcium pantothenate, 2% folic acid and 10% yeast rich in zinc (containing zinc 3000ppm), above percentage composition is quality percentage composition.
Preparation process:
1) according to above-mentioned formula ratio, various material mixing are even, and cross 100 mesh sieves after pulverizing with pulverizer;
2) dosing: water, as solvent, is made aaerosol solution by the powder obtaining;
3) by gained filled with solution in glass containers, autoclaving at 121 ℃;
Each step is all carried out in 300,000 grades of GMP workshops, to guarantee health and the safety of product.
Example 4
Material therefor: 15% beer yeast, 8% glutathion, 2% thiamine, 20% riboflavin, 20%VB
6, 1% nicotiamide, 15% calcium pantothenate, 1% folic acid, 5% yeast rich in selenium (containing selenium 2000ppm), 5% yeast rich in zinc (containing zinc 3000ppm) and 8% starch, above percentage composition is quality percentage composition.
Preparation process:
1) according to aforementioned proportion, various material mixing are even, and cross 100 mesh sieves after pulverizing with pulverizer;
2) 32% dextrin and 25% soluble starch are as excipient and dispersant, and 75% alcoholic solution, as wetting agent, is granulated when baking temperature is controlled at 55 ℃～65 ℃.
Each step is all carried out in 300,000 grades of GMP workshops, to guarantee health and the safety of product.
Example 5
Material therefor: 20% bakery yeast, 5% glutathion, 5% thiamine, 5% riboflavin, 10%VB
6, 10% nicotiamide, 15% calcium pantothenate, 5% folic acid, 10% yeast rich in selenium (containing selenium 2000ppm), 5% sucrose and 10% starch, above percentage composition is quality percentage composition.
Preparation process: direct powder compression
1) according to aforementioned proportion, various material mixing are even, and cross 100 mesh sieves after pulverizing with pulverizer;
2) by the abundant mix homogeneously of the powder obtaining;
3) directly powder is placed into and in tablet machine, carries out tabletting.
Each step is all carried out in 300,000 grades of GMP workshops, to guarantee health and the safety of product.
Three, the content measuring of various compositions and result
1. method of testing
Method of protein measurement adopts GB/T 5009.5-2003;
Thiamine assay method adopts GB/T 5009.84-2003;
Riboflavin assay method adopts GB/T 5009.85-2003;
VB
6assay method adopts GB/T 5009.84-2003;
Folic acid method adopts GB/T 5413.16-1997;
Nicotinic acid assay method adopts GB/T 5009.89-2003;
Pantothenic acid assay medium adopts GB/T 5413.17-1997;
Selenium assay method adopts GB/T 5009.93-2003;
Zinc assay method adopts GB/T 5009.14-2003;
The mensuration of glutathion adopts enzyme circulation kit measurement.
2. test result
The composition test of table 1 yeast product
Four, the detection of liver-protecting and alcoholism-relieving effect
1. experimental technique
Take and clean grade BALB/c mouse as animal subject, cause liver injury model (concentration of alcohol is 50%, mouse stomach amount 14mL/kgBW (body weight), the dosage of amounting to ethanol is 6000-7000mg/kgBW) with analytical pure ethanol.
A blank group (the normal mouse matched group without hepatic injury) is established in experiment, model control group (only cause hepatic injury and do not give the mice matched group of any product), market existing Chinese medicine liver-protecting and alcoholism-relieving product (alcohol decomposing tea made from kudzuvine flower, the Kang Yuan of Beijing Jiamei food technology development corporation, Ltd.), market existing beer yeast product (Jie Kangnuoshu brewer yeast egg albumen powder, Jie Nuo bio tech ltd, Shanghai) and example 1, 2, 3, 4, 5 yeast product test group, blank group mice and model control group mice do not give any existing product and product provided by the invention, alcohol decomposing tea made from kudzuvine flower and easypro brewer yeast egg albumen powder experimental mice give 5 times to the product of human body recommended amounts every day, example 1-5 experimental group gives 5 times of mices every day to the product provided by the invention of human body recommended amounts, wherein the recommended amounts of flakes product (example 1) is 50g/ every day, the recommended amounts of capsule product (example 2) is 0.5g/ every day, the recommended amounts of oral liquid (example 3) is 10mL (wherein containing dry 1g)/every day, (example 4) recommended amounts of grain products is 0.5 gram/every day, the recommended amounts of tablet (example 5) is 0.5g/ every day.
After animal model, with given the test agent, feed and put to death for 30 days, detect the content of malonaldehyde (MDA), reduced glutathion (GSH), triglyceride (TG) in hepatic tissue.Wherein MDA adopts MDA test kit (Shanghai Jie Mei gene Pharmaceutical Technology Co., Ltd) to measure, GSH adopts GSH test kit (Nanjing KaiJi Biology Science Development Co., Ltd) to measure, and TG adopts TG test kit (Beijing Jiuqiang Biotechnology Co., Ltd.) to measure.
2. experimental result
The impact (x+s, n=10) of table 2 hepatoprotective yeast product on murine liver tissue MDA, GSH, TG
*: compare P < 0.05 with model control group
Result decision principle: under the condition of setting up at model, between any one dosage group of model control group and given the test agent, have difference statistically, can be judged as positive findings.
As can be seen from Table 2, for MDA, the numerical value of model control group is far longer than the numerical value of blank group, after that is to say hepatic injury, MDA value is higher than normal value, the liver-protecting and alcoholism-relieving product of having bought from the market can be in various degree reduction MDA numerical value, but there is not statistical significant difference with model control group, and product provided by the invention and model control group are in a ratio of positive findings, MDA value significantly declines and has statistical significant difference, and visible product provided by the invention can reduce the MDA value after hepatic injury effectively.
For reduced form GSH, contrast model matched group and blank group data, can find out that hepatic injury meeting causes the remarkable decline of reduced form GSH value, between the liver-protecting and alcoholism-relieving product of having bought from the market and model control group data, there is not statistical significant difference, illustrate that existing product reports to the leadship after accomplishing a task to the effect of this index.Between the reduced form GSH value of product of the present invention and model control group, there is statistical significant difference, positive result, visible product provided by the invention is for stoping the decline of reduced form GSH numerical value to have active effects.
For TG, than blank group data, model control group numerical value significance increases, and illustrates that hepatic injury can cause the remarkable rising of this index, between the liver-protecting and alcoholism-relieving product of having bought from the market and model control group data, there is not significant difference, illustrate that they can not reduce this index effectively.Compare with model control group, product provided by the invention has significance,statistical meaning for this index, has significantly suppressed the rise of this index.
In sum, yeast product provided by the invention is compared with model control group, impact on the malonaldehyde in Mouse Liver, reduced glutathion, triglyceride has statistical significant difference, visible, and yeast product provided by the invention has obvious sobering up and liver protecting functions.And product of the present invention is better than the effect of existing product for the successful of above-mentioned three indexs, thus obtained a kind of All Pure Nature, efficient, novel, stablize, be easy to absorb take the relieving alcoholism and protecting the liver product that saccharomyces cerevisiae is primary raw material.
Above, embodiment based on certain preferred has been described the present invention, yet in the situation that without prejudice to the spirit and scope of the present invention defined in claims, can increase multiple variation and modification, this should be apparent for those skilled in the art.
Claims (6)
1. take the liver-protecting and alcoholism-relieving product that saccharomyces cerevisiae is primary raw material for one kind, it is characterized in that, the protein content > 42% of described saccharomyces cerevisiae, vitamin B group total content > 500mg/kg, glutathione content > 800mg/kg; In every gram of described liver-protecting and alcoholism-relieving product, contain 0.10～0.55g protein, 10～200000 μ g thiamines, 10～200000 μ g riboflavin, 10～200000 μ gVB6,1～50000 μ g folic acid, 1～200000 μ g nicotinic acid, 10～1000000 μ g pantothenic acid and 1～50000 μ g glutathion.
2. a method of preparing product described in claim 1, comprising: 1) saccharomyces cerevisiae is mixed to the emulsion that it is 10%-30% that water is mixed with containing dry with batching; 2) described emulsion is stirred; 3) vapour pressure at heated roller is under 0.5～0.65MPa, in cylinder, described emulsion is dried to flakes or is ground into Powderedly, wherein said batching is selected one or more components in the group that free vitamin B group, glutathion, yeast rich in selenium, yeast rich in zinc, sugarcane sugar and starch form.
3. a method of preparing product described in claim 1, comprising: 1) saccharomyces cerevisiae and batching are pulverized in pulverizer, the powder obtaining is crossed to 80 mesh sieves; 2) fully mix described powder, and minute install in capsule, wherein said batching is selected one or more components in the group that free vitamin B group, glutathion, yeast rich in selenium, yeast rich in zinc, sugarcane sugar and starch form.
4. a method of preparing product described in claim 1, comprising: 1) saccharomyces cerevisiae is mixed homogeneously and pulverized in pulverizer with batching, the powder obtaining is crossed 100 mesh sieves; 2) using described powder water as solvent, make aaerosol solution; 3) by described suspension filled with solution in vial, autoclaving at 121 ℃, wherein said batching is selected one or more components in the group that free vitamin B group, glutathion, yeast rich in selenium, yeast rich in zinc, sugarcane sugar and starch form.
5. a method of preparing product described in claim 1, comprising: 1) saccharomyces cerevisiae is mixed homogeneously with batching, and pulverize in pulverizer, the powder obtaining is crossed 100 mesh sieves; 2) adopt the soluble starch of 32% dextrin and 25% as excipient and dispersant, 75% alcoholic solution is as wetting agent, baking temperature is controlled at 55 ℃～65 ℃, described powder is granulated, and wherein said batching is selected one or more components in the group that free vitamin B group, glutathion, yeast rich in selenium, yeast rich in zinc, sugarcane sugar and starch form.
6. a method of preparing product described in claim 1, comprising: 1) saccharomyces cerevisiae and batching are pulverized in pulverizer, the powder obtaining is crossed 100 mesh sieves; 2) by the abundant mix homogeneously of the powder obtaining; 3) directly powder is placed into and in tablet machine, carries out tabletting, wherein said batching is selected one or more components in the group that free vitamin B group, glutathion, yeast rich in selenium, yeast rich in zinc, sugarcane sugar and starch form.
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Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102058047B (en) * | 2010-12-31 | 2012-12-12 | 济南圣泉唐和唐生物科技有限公司 | Health care product for relieving alcoholic liver |
| CN105054068A (en) * | 2014-01-25 | 2015-11-18 | 北京凯因科技股份有限公司 | Saccharomyces cerevisiae glutathione nutritional preparation |
| CN108236117A (en) * | 2017-10-13 | 2018-07-03 | 夏志君 | With yeast, its yeast protein after technology is processed can directly be eaten for food processing |
| CN107969707A (en) * | 2017-11-28 | 2018-05-01 | 夏志君 | Its yeast nucleic acid protein is most suitable for designed for old people after yeast protein is processed |
| CN109222089A (en) * | 2018-10-18 | 2019-01-18 | 上海梵怡生物科技有限公司 | A kind of the relieving alcoholism and protecting liver composition and preparation method of the alkanol containing rice bran fats |
| CN111375049B (en) * | 2018-12-29 | 2023-04-07 | 辽宁上药好护士药业（集团）有限公司 | Composition for relieving alcoholism and protecting liver as well as preparation method and application thereof |
| CN109846042A (en) * | 2019-01-22 | 2019-06-07 | 开封市良瑜秀泉食品有限公司 | The food compositions that one kind is relieved the effect of alcohol |
| CN110074392A (en) * | 2019-04-16 | 2019-08-02 | 吉林大学 | A kind of Antialcoholic liver-protecting nourishing the stomach shield intestines composition and preparation method thereof |
| CN110004092A (en) * | 2019-04-18 | 2019-07-12 | 郑州和合生物工程技术有限公司 | A kind of Lactobacillus casei, the preparation method and application of liver protection probiotic composition and the composition of detoxifying of relieving the effect of alcohol |
| CN111920052A (en) * | 2019-05-13 | 2020-11-13 | 安琪酵母股份有限公司 | Composition with functions of dispelling effects of alcohol and protecting liver and preparation method and application thereof |
| CN115919907A (en) * | 2021-08-31 | 2023-04-07 | E·施约林 | A composition for treating and/or preventing food and/or alcoholism |
| CN115364128A (en) * | 2022-10-21 | 2022-11-22 | 北京本草源生物科技有限公司 | Zinc-containing composition and liver-protecting wine and application thereof |
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| CN1840037A (en) * | 2006-01-27 | 2006-10-04 | 新疆医科大学 | Liver-protecting sobering-up capsule and method for preparing the same by utilizing grape peel |
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| CN1840037A (en) * | 2006-01-27 | 2006-10-04 | 新疆医科大学 | Liver-protecting sobering-up capsule and method for preparing the same by utilizing grape peel |
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