CN101829298B - Traditional Chinese medicine composition for treating primary dysmenorrheal and preparation method thereof - Google Patents
Traditional Chinese medicine composition for treating primary dysmenorrheal and preparation method thereof Download PDFInfo
- Publication number
- CN101829298B CN101829298B CN2009100793962A CN200910079396A CN101829298B CN 101829298 B CN101829298 B CN 101829298B CN 2009100793962 A CN2009100793962 A CN 2009100793962A CN 200910079396 A CN200910079396 A CN 200910079396A CN 101829298 B CN101829298 B CN 101829298B
- Authority
- CN
- China
- Prior art keywords
- parts
- chinese medicine
- medicine composition
- rhizoma
- traditional chinese
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention relates to a traditional Chinese medicine composition for treating primary dysmenorrheal, belonging to the field of traditional Chinese medicines. The traditional Chinese medicine composition for treating primary dysmenorrheal is prepared from active components comprising the following materials in parts by weight: 10-20 parts of Chinese angelica, 5-15 parts of rhizome of chuanxiong, 1-15 parts of nutgrass galingale rhizome, 5-10 parts of roots of three-nerved spicebush, 5-15 parts of curcuma zedoary, 5-10 parts of corydalis tubers and 5-15 parts of medicinal cyathula roots. The invention has the advantage that: with setting up a project for the really selected topic of the primary dysmenorrheal with more diseased symptoms and specificity shortage of therapeutic medicaments at present and making a law on the basis of the pathogenesis of main therapeutic symptoms, the invention not only takes the formation mechanism of qi depression to blood stasis into account, but also considers pathological results caused by the qi depression to blood stasis. Prescription formulation for medicine selection accords with the theories of traditional Chinese medicine, the traditional Chinese medicine composition has definite functional main treatment and symptom types of mainly treated diseases, the theories of traditional Chinese medicine science think that Chong-Ren disharmony and uterus qi and blood stases are caused by Chong and conception channel and qi-blood disharmony and pathogenic factor interference, qi and blood stases make women feel painful, which is the pathogenesis changes of dysmenorrheal, and the traditional Chinese medicine composition achieves the functions of removing stases and stopping pains through promoting flow of qi and blood circulation and has the advantages of principal and secondary treatment of diseases, definite therapeutic effect, rapid effect, favorable clinical effect and long-term and repeated application.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition for the treatment of primary dysmenorrhea and preparation method thereof, belong to technical field of Chinese medicines.
Background technology
Dysmenorrhea is meant menstrual cycle with spasmodic colic, is divided into two kinds of constitutional and Secondary cases.Primary dysmenorrhea is meant that the genitals does not have the dysmenorrhea of organic disease; Secondary dysmenorrhea is then many to be had due to the organic disease because of the genitals.
Show that according to sampling survey primary dysmenorrhea is sent out the adolescence women in 6 months to 2 years after 15～25 years old and menophania well, about 50%～90%, 37%～55% the patient of its sickness rate influences life, study and activity.
Pointed out 80% dysmenorrhea that has in various degree among the global women according to Britain one tame authorities,medical mechanism investigation report in 2004;
The reports such as Patel V of Britain in 2006,2262 18～45 years old women of investigation India fruit Ah suffer from the dysmenorrhea number and surpass half, and 37% women arrives the severe pain warp in being.Osmanli Cakir M in 2007 etc. investigate the schoolgirl of colleges and universities, and report primary dysmenorrhea sickness rate is 89.5%.Introduce in " female chronic pelvic pain " book, the Britain women of child-bearing age 12% have serious dysmenorrhea, and 45% has moderate to the severe pain warp.It is reported that the U.S. has among the women of periodically menstruation 90% dysmenorrhea is arranged, 36% has dysmenorrhea always or often.
Dysmenorrhea is periodical attack and vicious cycle, tend to cause patient's psychasthenia, make the patient suffering hard to bear, prolonged and repeated outbreak also can influence mental health, the not normal of psychological behavior appears, such as agitation, irritability, depression, absent-minded, severe patient even crime and impulsive behavior can occur.
Hence one can see that, and dysmenorrhea is a disease comparatively general in women's illness, and harm is serious.
Western medicine thinks, the generation of primary dysmenorrhea and genitals's local patholoic change and endocrine, nervous and mental factors are relevant, mainly is owing to strength of uterine contraction strengthens, uterine blood flow reduces and the influence of pituitary hormone, the effect of neurotransmitter cause.Doctor trained in Western medicine adopts spasmolytic, analgesic and PGSI for TREATMENT OF DYSMENORRHOEA more, the short-term good analgesic effect, but can not solve root problem, and of short duration, the easy recurrence of curative effect, side effect is bigger, makes clinical practice be subjected to certain limitation.
Relieving spasm to stop pain class medicine usually before menstrual onset 12～24 hours oral, mainly comprise salicylic acid, hormones, anti-steroid anti-inflammatory type etc., this class medicine is the pain symptom of relieving dysmenorrhea effectively, can not fundamentally eliminate the cause of disease of dysmenorrhea, but its corresponding side reaction is unsuitable for patient's prolonged application as nauseating, vomiting, anaphylaxis, blood coagulation disorders, stomachache, menoxenia etc.If calm class drug main is at the patient of serious dysmenorrhea companion psychentonia, emotional instability, it represents medicine is chlorpromazine, and that the side effect that it is followed is mainly is drowsiness, tachycardia, xerostomia and constipation, postural hypotension etc., and prolonged application can cause breast swelling, amenorrhea and decreased growth etc.Endocrine therapy, generally select diethylstilbestrol, norethindrone, Norethisterone Compound sheet etc. for use, this class medicine is at the clinical recovery normal menstrual cycle that is mainly used in, therapeutic effect for primary dysmenorrhea is not good, relapse rate after the treatment is very high, because of its side reaction shows as nauseating, vomiting, anorexia, headache, dizziness, irregular colporrhagia, metrauxe, frequent micturition or painful urination etc. should not be taken for a long time.The main medicine of prostaglandin antagonist has ibuprofen (ibuprofen), naproxen (naproxan), mefenamic acid (mefenamic acid), it mainly is purpose with the pain relieving, though pain relieving is rapid, but prolonged application can develop immunity to drugs, addiction, gastrointestinal ulceration, hepatic and renal function injure, reach serious adverse reactions such as central nervous system disorder, and relapse rate is higher.
Theory of Chinese medical science thinks that the pathogenic factor of dysmenorrhea is a lot, but its pathogenesis is nothing more than " stagnation of QI and blood may bring about pain " or " not Rong Ze pain ".Tradition traditional Chinese medical herbal treatment dysmenorrhea is with a long history, determined curative effect, many Chinese medicines no matter be single or compound preparation all to improve through preceding or menstrual period few abdomen pain, distending pain of the breast, dysmenorrhea symptom such as do not relax uncomfortable in chest have remarkable effect, and curative effect is lasting, does not recur after the drug withdrawal.Thereby be subjected to the great kindness of extensive patients deeply.Development along with transition of age and society, the pathogenic factors of dysmenorrhea and the performance of clinical disease have had certain change, wherein in the majority with the patient of qi stagnation and blood stasis type primary dysmenorrhea, yet, also few through the tcm product of its determined curative effect of standardization clinical verification at present, and control from cold coagulation legislation opinion mostly, cause kind that dysmenorrhea takes place seldom, can't satisfy patient's actual demand at the stagnation of QI, the stagnation of QI.Main herbal species has TIANQI TONGJING JIAONANG, YUANHU ZHITONG PIAN, norlutin, TONGJINGBAO KELI, dysmenorrhea spirit granule and HUAHONG PIAN on the market.TIANQI TONGJING JIAONANG is primarily aimed in the cold-condensing type dysmenorrhea, uses the hot class medicine that looses of a large amount of fragrance, easily consumes gas; YUANHU ZHITONG PIAN is mainly used in stomachache, hypochondriac pain, headache and the menstrual pain of caused by energy stagnation and blood stasis, lacks specific aim, can cause feeling sick, dizzy, weak, excessively performances such as respiration inhibition, parkinson occur.Norlutin mainly is applicable to the menoxenia and the dysmenorrhea of deficiency of both QI and blood.TONGJINGBAO KELI is directed to menorrhalgia of cold and qi stagnation type, adopts a large amount of removing blood stasis medicines in the side, easily causes patient's blood deficiency symptom in treatment.Dysmenorrhea spirit granule is primarily aimed in the caused by energy stagnation and blood stasis dysmenorrhea, but square taste of Chinese medicine is various, and dose is big.HUAHONG PIAN mainly is applicable to secondary row dysmenorrhea, can not satisfy primary dysmenorrhea patient's demand.
The characteristics research and development that the present invention is based on qi stagnation and blood stasis type constitutional pain high incidence and shortage specific aim medicine form.
The present invention has determined curative effect, and effect is rapid, and clinical producing effects well used the low characteristics of no addiction and relapse rate for a long time, repeatedly.
Summary of the invention
The technical problem to be solved in the present invention is: Chinese medicine composition and preparation thereof that a kind of effective treatment qi stagnation and blood stasis type primary dysmenorrhea is provided.
Another technical problem that the present invention will solve is: the preparation method that aforementioned pharmaceutical compositions is provided.
For achieving the above object, the present invention is by the following technical solutions:
A kind of Chinese medicine composition for the treatment of primary dysmenorrhea, its active component is made by following bulk drugs:
5～15 parts of 5～15 portions of Rhizoma Cyperis of 10～20 parts of Rhizoma Chuanxiongs of Radix Angelicae Sinensis
5～10 parts of 5～15 parts of Rhizoma Corydalis of 5～10 parts of Rhizoma Curcumae of the Radix Linderae
5～15 parts of Radix Cyathulaes.
Described Chinese medicine composition is an oral formulations, as granule, drop pill, capsule, pill, tablet, oral liquid etc.
A kind of preparation method for the treatment of the Chinese medicine composition of primary dysmenorrhea: above seven flavors, get Radix Angelicae Sinensis, Rhizoma Chuanxiong, Rhizoma Cyperi, the Radix Linderae and Rhizoma Curcumae and be ground into coarse powder, add 6～10 times of amounts of water, soaked 0.5～3 hour, hydrodistillation extracts volatile oil, extraction time is 3～6 hours, collects volatile oil, and the aqueous solution after distillation device is in addition collected; Medicinal residues add 6～10 times of water gagings and decocted 0.5～2 hour, filter, and filtrate and above-mentioned aqueous solution merge, and filtrate is concentrated into relative density 1.00～1.30 (50～80 ℃); Radix Cyathulae, Rhizoma Corydalis add 6～10 times of amount alcohol reflux of 60%～75% three times, each 0.5～2 hour, filter, merging filtrate reclaims ethanol, merges with above-mentioned concentrated solution, be evaporated to relative density 1.10～1.50 (50～80 ℃), drying is pulverized.Add above-mentioned volatile oil, make peroral dosage form with common process.
It is acceptable that described adjuvant is selected from pharmaceutics: one or more in sucrose, dextrin, microcrystalline Cellulose, starch, lactose, xylitol, chitin, high fructose, cyclamate, polyethylene glycol 6000, calcium carbonate, magnesium stearate, gelatin, hydroxy methocel, micropowder silica gel, silicon dioxide, hydroxypropyl cellulose, sodium carboxymethyl cellulose, agar, gelatin, methylcellulose, ethyl cellulose, hydroxypropyl emthylcellulose or the carboxymethyl starch sodium.
Compared with prior art, the present invention has following advantage:
1, from curing mainly the etiology and pathogenesis legislation of card, both considers the formation mechanism of qi stagnation and blood stasis type primary dysmenorrhea, taken the pathological examination that causes therefrom again into account.Prescription is selected medicine and is met Chinese medical theory, function cure mainly and the syndrome type of main disease to be treated clear and definite, qi-blood disharmony based on dash appointing, pathogen disturb cause dash appoint, the uterus stagnation of QI-blood, stagnation of QI and blood may bring about pain, the pathogenesis of sending out to dysmenorrhea changes, and reaches the merit of stasis-dispelling and pain-killing, treating both the principal and secondary aspects of a disease by promoting flow of QI and blood, determined curative effect, effect is rapid, and clinical producing effects well used no addiction for a long time, repeatedly.
2, the Radix Angelicae Sinensis chosen of the present invention, Rhizoma Chuanxiong altogether for monarch drug with promoting flow of QI and blood, nourishing blood for regulating menstruation, eliminating stasis to stop pain; Rhizoma Cyperi, the Radix Linderae, regulating qi-flowing for activating stagnancy, dispersing cold for relieving pain, the merit of enhancing combing mechanism of qi; Rhizoma Curcumae is the gas medicine in the blood, blood-activating and qi-promoting, Rhizoma Corydalis can be gone into liver, pericardium channel is walked blood system, can go into spleen again, lung meridian is walked edema caused by disorder of QI, more than be minister altogether, principal drug assistance circulation of qi promoting, the merit of invigorating blood circulation; Assistant is with Radix Cyathulae promoting blood circulation to restore menstrual flow, conducting blood to flow downwards, to reach general rule not bitterly; All medicines share, and make channels and collaterals dredging, and the QI and blood smoothness is dashed and appointed fosterly, and pain must be removed, and plays the effect of circulation of qi promoting and blood, stasis-dispelling and pain-killing altogether.
3, the present invention comes from clinical practice for many years, and clinical observation confirms that it can effectively solve the cause of disease of qi stagnation and blood stasis type primary dysmenorrhea, and can improve fast that lower abdomen has an intense pain, the soreness of waist, nausea and vomiting, extremity are not warm, dysmenorrhea symptoms such as dripping cold sweat, and do not have any toxicity.
The invention will be further described below in conjunction with the specific embodiment; be not the qualification to invention, according to prior art well known in the art, embodiments of the present invention are not limited to this; therefore all this areas of having done according to present disclosure be equal to replacement, all belong to protection scope of the present invention.
The specific embodiment
Embodiment 1: the granule preparation
One. prescription
Radix Angelicae Sinensis 10kg Rhizoma Chuanxiong 5kg Rhizoma Cyperi 5kg Radix Linderae 5kg
Rhizoma Curcumae 5kg Rhizoma Corydalis 5kg Radix Cyathulae 5kg.
Two. method for making
More than seven flavors, get Radix Angelicae Sinensis, Rhizoma Chuanxiong, Rhizoma Cyperi (processed with vinegar), the Radix Linderae and Rhizoma Curcumae and be ground into coarse powder, add 6 times of amounts of water, soaked 0.5 hour, hydrodistillation extracts volatile oil, extraction time is 3 hours, collects volatile oil, the device collection in addition of the aqueous solution after the distillation; Medicinal residues add 6 times of water gagings and decocted 0.5 hour, filter, and filtrate and above-mentioned aqueous solution merge, and filtrate is concentrated into relative density 1.00～1.30 (70 ℃); Radix Cyathulae, Rhizoma Corydalis add 6 times of amount alcohol reflux of 60% three times, and each 0.5 hour, filter, merging filtrate reclaims ethanol, merges with above-mentioned concentrated solution, is evaporated to relative density 1.15～1.40 (70 ℃), and drying is pulverized.Get 1 part of dried cream powder, 1 part of sucrose, 0.5 part in dextrin adds 70% ethanol and makes granule in right amount, and drying adds above-mentioned volatile oil, mixing, promptly.Granule dosage: 9g/ bag (the about 15.6g of symphysis medicine).
Embodiment 2: the granule preparation
One. prescription
Radix Angelicae Sinensis 20kg Rhizoma Chuanxiong 15kg Rhizoma Cyperi 15kg Radix Linderae 10kg
Rhizoma Curcumae 15kg Rhizoma Corydalis 10kg Radix Cyathulae 15kg.
Two. method for making
More than seven flavors, get Radix Angelicae Sinensis, Rhizoma Chuanxiong, Rhizoma Cyperi (processed with vinegar), the Radix Linderae and Rhizoma Curcumae and be ground into coarse powder, add 10 times of amounts of water, soaked 3 hours, hydrodistillation extracts volatile oil, extraction time is 6 hours, collects volatile oil, the device collection in addition of the aqueous solution after the distillation; Medicinal residues add 10 times of water gagings and decocted 2 hours, filter, and filtrate and above-mentioned aqueous solution merge, and filtrate is concentrated into relative density 1.05～1.20 (70 ℃); Radix Cyathulae, Rhizoma Corydalis add 10 times of amount alcohol reflux of 75% three times, and each 2 hours, filter, merging filtrate reclaims ethanol, merges with above-mentioned concentrated solution, is evaporated to relative density 1.15～1.40 (70 ℃), and drying is pulverized.Get 1 part of dried cream powder, 1 part of lactose, 0.5 part in two qi dextrin add 70% ethanol and make granule in right amount, and drying adds above-mentioned volatile oil, mixing, promptly.Granule dosage: 9g/ bag (the about 15.6g of symphysis medicine).
Embodiment 3: the granule preparation
One. prescription
Radix Angelicae Sinensis 15kg Rhizoma Chuanxiong 10kg Rhizoma Cyperi 10kg Radix Linderae 7kg
Rhizoma Curcumae 7kg Rhizoma Corydalis 7kg Radix Cyathulae 10kg
Two. method for making
More than seven flavors, get Radix Angelicae Sinensis, Rhizoma Chuanxiong, Rhizoma Cyperi (processed with vinegar), the Radix Linderae and Rhizoma Curcumae and be ground into coarse powder, add 8 times of amounts of water, soaked 2 hours, hydrodistillation extracts volatile oil, extraction time is 6 hours, collects volatile oil, the device collection in addition of the aqueous solution after the distillation; Medicinal residues add 8 times of water gagings and decocted 1 hour, filter, and filtrate and above-mentioned aqueous solution merge, and filtrate is concentrated into relative density 1.10～1.35 (70 ℃); Radix Cyathulae, Rhizoma Corydalis add 8 times of amount alcohol reflux of 70% three times, and each 1 hour, filter, merging filtrate reclaims ethanol, merges with above-mentioned concentrated solution, is evaporated to relative density 1.15～1.45 (70 ℃), and drying is pulverized.Get 1 part of dried cream powder, 1 part of sucrose, 0.5 part of microcrystalline Cellulose adds 70% ethanol and makes granule in right amount, and drying adds above-mentioned volatile oil, mixing, promptly.Granule dosage: 9g/ bag (the about 15.6g of symphysis medicine).
Embodiment 4, pharmacodynamics test
Test material: the present invention adopts the granule of embodiment 2, and 9g/ bag (the about 15.6g of symphysis medicine) is to call " Radix Angelicae Sinensis dysmenorrhea granule " in the following text; Dysmenorrhea spirit granule, lot number: 0705078, provide by the Beijing Yadong Biology Pharmacy Co., Ltd.
One, analgesic activity:
Experimental animal: Kunming mouse, body weight 20 ± 2g.
The test grouping: each experimental animal is divided into 5 groups at random, 10 every group.
Trial drug dosage: positive controls: dysmenorrhea spirit granule 10g/kg (concentration 50%); The administration group of large, medium and small dosage is irritated stomach respectively and is given Radix Angelicae Sinensis dysmenorrhea granule 1g/kg, 2g/kg, 4g/kg (concentration is respectively 5%, 10%, 20%), and 0.4ml/20g is pressed in administration; Blank group: irritate stomach and give isopyknic water.Every day 1 time, successive administration 3 days.
1, hot plate method:
Test method: get 50 of female kunming mices.Water bath with thermostatic control is adjusted to 55 ± 0.5 ℃, and with the bottom contact water surface of beaker, the heating back is as thermostimulation.Each 1 of mice is placed in the beaker, from being placed on the hot plate to occurring licking the metapedes required time as this pain threshold, all pain thresholds less than 5 seconds, give it up greater than 30 seconds or leaper.
30min, 60min, 90min survey the pain threshold of mice after the last administration, surpass 60s in 60s.
The results are shown in Table 1.Compare with the blank group, the threshold of pain of 30min all has significant difference (P＜0.05) with it after the administration of the particulate large, medium and small dosage group of Radix Angelicae Sinensis dysmenorrhea; 60min after the administration, the threshold of pain of large, medium and small dosage group all has significant difference (P＜0.01) with it; 90min after the administration, the threshold of pain of middle dosage group has the threshold of pain of significant difference (P＜0.05), heavy dose of group that significant difference (P＜0.01) is arranged with it with it.Three dosage groups of Radix Angelicae Sinensis dysmenorrhea granule are compared difference that there are no significant.
The influence of the table 1 pair mice thermostimulation threshold of pain (x ± SD)
Compare * P＜0.05, * *＜0.01 with the blank group
2, acetic acid twisting method:
Test method: 50 of healthy kunming mices, male and female half and half.After last administration half an hour, ip0.6% acetic acid 0.1ml/10g observes and writes down mouse writhing reaction times in the injection acetic acid 20 minutes immediately.
The results are shown in Table 2, compare the large, medium and small dosage group of Radix Angelicae Sinensis dysmenorrhea granule with the blank group and turn round body time number average minimizing, wherein big or middle dosage group has significant difference.
The influence of table 2 pair mice acetic acid twisting reaction (x ± SD)
3, oxytocin brings out dysmenorrhea model in mice:
Test method: 50 of Healthy female kunming mices, each is organized mouse stomach and gives diethylstilbestrol, once a day, each 2mg/kg, after continuous 10 days, beginning in the 11st day is respectively organized mice and is given diethylstilbestrol afternoon, each 2mg/kg, simultaneously, positive controls gives dysmenorrhea spirit granule, and the administration group of three dosage is irritated stomach respectively and given Radix Angelicae Sinensis dysmenorrhea granule, for three days on end, ip oxytocin 20u/kg behind the last administration 30min, observed and recorded respectively organize and turn round body incubation period in the mice 30min and turn round the body number of times.
The results are shown in Table 3, compare with the blank group, all prolong the incubation period of the large, medium and small dosage group of Radix Angelicae Sinensis dysmenorrhea granule, and wherein heavy dose of group has significant difference; The large, medium and small dosage of Radix Angelicae Sinensis dysmenorrhea granule is turned round body time number average and is reduced, and wherein big or middle dosage group has significant difference.
Table 3 pair oxytocin brings out the influence (x ± SD) of dysmenorrhea model in mice writhing response
Compare with the blank group,
*P＜0.05,
*PP＜0.01
Two, diastole uterine smooth muscle effect:
Test method:
1. unlatching instrument, rectifying an instrument and setting bath recirculated water heating-up temperature is 37 ℃.
2. get the female not pregnant rat of 220～280g, 48h ip diethylstilbestrol 2mg/kg before the experiment causes artificial rutting period to improve the sensitivity of uterus to medicine, and water is can't help in 24h fasting before the experiment.The same day was taken out the uterus rapidly with 20% urethanes 6ml/kg anesthetized rat in experiment, and immersing temperature immediately is in 37 ± 0.5 ℃ of Rockwell liquid, rinsed, and separated fatty tissue on every side, made the uterus muscle bar that is about 2cm.
3. the uterus muscle bar is hung in the bath that fills Rockwell liquid, continue to pass to 95% O
2And 5%CO
2Mist, 60～80 bubbles of per minute, uterus muscle bar upper end connects tonotransducer, and bath is fixed in the lower end, and adjusting knob degree of tightness to uterus muscle bar tension force is shown as 2g.
With the uterus muscle bar in nutritional solution about balance 30min, change liquid, again write down one section normalized curve, when the uterus muscular tension peaks, each specimen of administration group adds medicinal liquid 0.4ml, (make the interior large, medium and small dosage group concentration of groove be respectively 0.1%, 0.2%, 0.4%, positive controls concentration is 1%).The blank group adds isopyknic distilled water.Observe the relatively fall of administration group and blank group specimen 3min, 6min, 9min after administration, and obtain the spasmolytic rate of medicine, comparable group differences by following formula:.
Amplitude * 100% before spasmolytic rate=(amplitude after the preceding amplitude-administration of administration)/administration.
The results are shown in Table 4, compare with the blank group, the spasmolytic rate of 3min, 6min all has significant difference with it after the administration of the big or middle dosage group of Radix Angelicae Sinensis dysmenorrhea granule, and small dose group does not have significant difference with it; During 9min, middle dosage group spasmolytic rate all has significant difference with it, large and small dosage group there was no significant difference after the administration.Three dosage groups of Radix Angelicae Sinensis dysmenorrhea granule are compared, and do not have significant difference.
The table 4 pair tensile influence of rat uterus smooth muscle (x ± SD)
Compare * P＜0.05, * *＜0.01 with the blank group
Three, to uterine smooth muscle PGF
2aAnd PGE
2The influence of content:
Test method: choose 60 of healthy Wistar rats, female unpregnancy, body weight 160～220g, divide 6 groups at random, positive controls gives dysmenorrhea spirit granule 5g/kg (concentration 50%), the administration group of three dosage is irritated stomach respectively and is given Radix Angelicae Sinensis dysmenorrhea granule 0.5g/kg, 1g/kg, (concentration is respectively 5% to 2g/kg, 10%, 20%), administration volume 2ml/200g, blank group and model group are irritated stomach by 2ml/200g and are given water, once a day, continuous 7 days, from the 4th day of administration, the all continuous ip of the rat of all the other 5 groups gave diethylstilbestrol 4 days (0.8mg/ days except that the blank group, 0.4mg/ my god, 0.4mg/ my god, 0.8mg/ my god), cause artificial rutting period to improve the sensitivity of uterus to medicine, blank group ip gives isopyknic normal saline.The 8th day with 20% urethanes 6ml/kg anesthetized rat, the separating uterus tissue, the uterus is divided into two parts, rapid weighing, rapidly it is put in the homogenizer, a part adds homogenate PBS solution (PH=7.4) by the 2ml/100mg uterus weight and is developed into homogenate, the centrifugal 20min of 3000r/min, get supernatant, press PGF again
2aOperating procedure is measured PGF on the uterine smooth muscle
2aContent; Another part adds normal saline by the 2ml/100mg uterus weight and is developed into homogenate, and the centrifugal 20min of 3000r/min gets supernatant, presses PGE again
2Operating procedure is measured PGE on the uterine smooth muscle
2Content.
The results are shown in Table 5, compare, PGF after the administration of dysmenorrhea model group with the blank group
2aCertain rising is arranged, PGE
2Bigger reduction is arranged; Compare with model group, in the Radix Angelicae Sinensis dysmenorrhea granule, the PGF of group
2aContent has reduction trend, large, medium and small dosage group PGE
2Content has rising trend.
Above-mentioned pharmacodynamic study result shows, Radix Angelicae Sinensis dysmenorrhea granule is respectively with mice 1g/kg, 2g/kg, 4g/kg, rat 0.5g/kg, 1g/kg, 2g/kg irritate stomach, pain threshold that can the significant prolongation mice to thermostimulation, obviously suppress the acetic acid twisting reaction of mice, to the dysmenorrhea model mice due to the oxytocin can the significant prolongation writhing response incubation period, the mouse writhing number of times also has remarkable minimizing simultaneously; For 48h ip diethylstilbestrol 2mg/kg before the experiment, cause artificial rutting period to improve the female not pregnant rat of uterus to the sensitivity of medicine, make the interior root product concentration of bath reach respectively at 0.1%, 0.2%, 0.4% o'clock, its isolated uterine flesh bar tension force is reduced rapidly, the spasmolytic rate is significantly increased, and can make in advance dysmenorrhea model P of Rats GF due to the ip diethylstilbestrol in addition
2aRaise to some extent, PGE
2There is the situation of bigger reduction to obtain to a certain degree reverse.Confirm the effect that medicine of the present invention has the obvious treatment dysmenorrhea.
Claims (6)
1. Chinese medicine composition for the treatment of primary dysmenorrhea, its active component is made by following bulk drugs:
5～15 parts of 5～15 portions of Rhizoma Cyperis of 10～20 parts of Rhizoma Chuanxiongs of Radix Angelicae Sinensis
5～10 parts of 5～15 parts of Rhizoma Corydalis of 5～10 parts of Rhizoma Curcumae of the Radix Linderae
5～15 parts of Radix Cyathulaes;
More than seven flavors, get Radix Angelicae Sinensis, Rhizoma Chuanxiong, Rhizoma Cyperi, the Radix Linderae and Rhizoma Curcumae and be ground into coarse powder, add 6～10 times of amounts of water, soaked 0.5～3 hour, hydrodistillation extracts volatile oil, extraction time is 3～6 hours, collects volatile oil, the device collection in addition of the aqueous solution after the distillation; Medicinal residues add 6～10 times of water gagings and decocted 0.5～2 hour, filter, and filtrate and above-mentioned aqueous solution merge, and relative density 1.00～1.30 when filtrate was concentrated into 50～80 ℃; Radix Cyathulae, Rhizoma Corydalis add 6～10 times of amount alcohol reflux of 60%～75% three times, and each 0.5～2 hour, filter, merging filtrate reclaims ethanol, merges with above-mentioned concentrated solution, and relative density 1.10～1.50 when being evaporated to 50～80 ℃, and drying is pulverized; Add above-mentioned volatile oil, make peroral dosage form with common process.
2. a kind of Chinese medicine composition for the treatment of primary dysmenorrhea according to claim 1 is characterized in that: described peroral dosage form is granule, capsule, pill, tablet, drop pill or oral liquid.
3. a kind of Chinese medicine composition for the treatment of primary dysmenorrhea according to claim 1 is characterized in that: described peroral dosage form is a granule.
4. according to any one described a kind of Chinese medicine composition for the treatment of primary dysmenorrhea in the claim 1 to 3, it is characterized in that: also contain adjuvant in the described pharmaceutical composition.
5. a kind of Chinese medicine composition for the treatment of primary dysmenorrhea according to claim 4, it is characterized in that: it is acceptable that described adjuvant is selected from pharmaceutics: one or more in sucrose, dextrin, microcrystalline Cellulose, starch, lactose, xylitol, chitin, high fructose, cyclamate, polyethylene glycol 6000, calcium carbonate, magnesium stearate, gelatin, hydroxy methocel, micropowder silica gel, silicon dioxide, hydroxypropyl cellulose, sodium carboxymethyl cellulose, agar, methylcellulose, ethyl cellulose, hydroxypropyl emthylcellulose or the carboxymethyl starch sodium.
6. any one described a kind of preparation method for the treatment of the Chinese medicine composition of primary dysmenorrhea in the claim 1 to 3, it is characterized in that: above seven flavors, get Radix Angelicae Sinensis, Rhizoma Chuanxiong, Rhizoma Cyperi, the Radix Linderae and Rhizoma Curcumae and be ground into coarse powder, add 6～10 times of amounts of water, soaked 0.5～3 hour, hydrodistillation extracts volatile oil, and extraction time is 3～6 hours, collect volatile oil, the aqueous solution after distillation device is in addition collected; Medicinal residues add 6～10 times of water gagings and decocted 0.5～2 hour, filter, and filtrate and above-mentioned aqueous solution merge, and relative density 1.00～1.30 when filtrate was concentrated into 50～80 ℃; Radix Cyathulae, Rhizoma Corydalis add 6～10 times of amount alcohol reflux of 60%～75% three times, and each 0.5～2 hour, filter, merging filtrate reclaims ethanol, merges with above-mentioned concentrated solution, and relative density 1.10～1.50 when being evaporated to 50～80 ℃, and drying is pulverized; Add above-mentioned volatile oil, make peroral dosage form with common process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100793962A CN101829298B (en) | 2009-03-10 | 2009-03-10 | Traditional Chinese medicine composition for treating primary dysmenorrheal and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100793962A CN101829298B (en) | 2009-03-10 | 2009-03-10 | Traditional Chinese medicine composition for treating primary dysmenorrheal and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101829298A CN101829298A (en) | 2010-09-15 |
| CN101829298B true CN101829298B (en) | 2011-12-07 |
Family
ID=42713620
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009100793962A Active CN101829298B (en) | 2009-03-10 | 2009-03-10 | Traditional Chinese medicine composition for treating primary dysmenorrheal and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101829298B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102793884B (en) * | 2012-09-05 | 2014-01-29 | 山东省中医药研究院 | Chinese medical plaster for treating dysmenorrhea and preparation method thereof |
| CN103893676B (en) * | 2012-12-27 | 2016-06-01 | 北京创立科创医药技术开发有限公司 | The preparation technology of a kind of Chinese medicine composition treating primary dysmenorrhoea |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1686296A (en) * | 2005-03-30 | 2005-10-26 | 何周荣 | Medicinal composition for treating soreness, its preparation method and application |
| CN101095930A (en) * | 2007-07-12 | 2008-01-02 | 张琴 | Olibanum zedoary plaster for treating menorrhalgia and the preparation technics thereof |
-
2009
- 2009-03-10 CN CN2009100793962A patent/CN101829298B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1686296A (en) * | 2005-03-30 | 2005-10-26 | 何周荣 | Medicinal composition for treating soreness, its preparation method and application |
| CN101095930A (en) * | 2007-07-12 | 2008-01-02 | 张琴 | Olibanum zedoary plaster for treating menorrhalgia and the preparation technics thereof |
Non-Patent Citations (1)
| Title |
|---|
| 谢威等.原发性痛经中医治疗进展.《实用中医药杂志》.2008,第24卷(第11期),748-749. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101829298A (en) | 2010-09-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102266514A (en) | Chinese medicinal composition for treating acute and chronic gastritis and preparation method thereof | |
| CN102988675B (en) | Traditional Chinese medicine combination for treating chronic prostatitis and preparation method thereof | |
| CN101181568A (en) | Traditional chinese medicine preparation for curing osteoproliferation as well as arthrosis ache | |
| CN101757281B (en) | Traditional Chinese medicine composition for treating gastrointestinal disease and preparation method thereof | |
| CN101700336B (en) | Chinese herba preparation for curing craniocerebra trauma residual headache and preparation method | |
| CN101829298B (en) | Traditional Chinese medicine composition for treating primary dysmenorrheal and preparation method thereof | |
| CN100502843C (en) | Chinese medicine for treating mammary gland cystoma, mammitis and preparation method of its film coated tablet | |
| CN100450517C (en) | Dysmenorrhea treating Chinese traditional medicine preparation | |
| CN102430009B (en) | Traditional Chinese medicinal composition for treating gastrointestinal disease and preparation method thereof | |
| CN103520646B (en) | Chinese medicine composition for treating depression and preparation method of Chinese medicine composition | |
| CN101810843B (en) | Medicament for treating nerve system diseases such as cerebrovascular accident, spinal cord injury and senile dementia | |
| CN1985982B (en) | Medicine for treating colitis and its preparing method | |
| CN101129492B (en) | Traditional Chinese medicine formulated product for treating melancholia | |
| CN100486626C (en) | A medicament for treating tension headache and preparation method thereof | |
| CN1579485B (en) | Traditional Chinese medicinal composition for treating intestinal function disorder and its preparation method | |
| CN102430008A (en) | Chinese medicinal composition for treating gastrointestinal disease and preparation method thereof | |
| CN104547709A (en) | Chinese medicinal composition for treating meniere disease as well as preparation method and application thereof | |
| CN102416088B (en) | Chinese medicinal composition for treating gastrointestinal diseases and preparation method thereof | |
| CN108524658B (en) | Traditional Chinese medicine for treating restless legs syndrome and preparation method thereof | |
| CN112089783B (en) | Application of traditional Chinese medicine composition in preparation of medicine for preventing or/and treating obesity | |
| CN100563685C (en) | A kind of Chinese patent medicine-Herba Cistanches relieving constipation preparation for the treatment of chronic constipation | |
| CN101264302A (en) | Application of Chinese medicinal composition in preparing medicaments for treating menoxenia and menorrhalgia | |
| CN102283980B (en) | Medicament for treating damp and heat swelling type chronic kidney disease | |
| CN100998784A (en) | Traditional Chinese medicine composition tablet for treating gastrointestinal disease and its preparing method | |
| CN1666763A (en) | Dripping pills of six Ingredients with Rehmanniae |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| ASS | Succession or assignment of patent right |
Owner name: BEIJING SUPERTRACK MEDICAL TECHNOLOGY DEVELOPMENT Free format text: FORMER OWNER: BEIJING CAKING SCIENCE AND TECHNOLOGY CO., LTD. Effective date: 20110527 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 100097 10-1023, JIAYOU INTERNATIONAL BUILDING, NO. 25, LANDIANCHANG SOUTH ROAD, HAIDIAN DISTRICT, BEIJING TO: 100029 MAILBOX 91, BEIJING UNIVERSITY OF CHINESE MEDICINE, NO. 11, NORTH 3RD RING ROAD EAST, BEIJING |
|
| TA01 | Transfer of patent application right |
Effective date of registration: 20110527 Address after: 91, box 11, Beijing University of Chinese Medicine, 100029 East Third Ring Road, Beijing Applicant after: Beijing Chuangli-Kechuang Pharmacy Technology Development Co., Ltd. Address before: 100097 Beijing city Haidian District landianchang Road No. 25 chiayo International Building 10-1023 Applicant before: Beijing Kaijin Science & Technology Co., Ltd. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |