CN101824386A - Chlorella and application thereof - Google Patents
Chlorella and application thereof Download PDFInfo
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- CN101824386A CN101824386A CN201019026021A CN201019026021A CN101824386A CN 101824386 A CN101824386 A CN 101824386A CN 201019026021 A CN201019026021 A CN 201019026021A CN 201019026021 A CN201019026021 A CN 201019026021A CN 101824386 A CN101824386 A CN 101824386A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/59—Biological synthesis; Biological purification
Abstract
The invention discloses a strain of chlorella, which is classified to be named as chlorella vulgaris and is preserved in China Centre for Type Culture Collection, and the preservation number is CCTCC NO.: M 209256. The invention also discloses the application of the chlorella in producing microalgae oil and fixing CO2. The chlorella can endure 24.5% of CO2 concentration, the content of grease can reach 17-31%, and the invention has higher CO2 fixing efficiency than ordinary seawater algae, and can obtain high-yield microalgae oil by selecting appropriate culture conditions, so the culture cost of the microalgae can be greatly reduced, and a good production strain is provided for preparing biodiesel by the microalgae oil.
Description
Technical field
The invention belongs to little algae biological technical field, be specifically related to a chlorella and application thereof.
Background technology
The exploitation and the use of tradition fossil resource have promoted socio-economic development, have also produced a large amount of CO simultaneously
2Isothermal chamber gas, thus make global environment further worsen, calamitous weather takes place frequently, serious threat social Sustainable development.According to World Bank's statistics, in the middle of in the past 30 years, because of CO
2Excessively discharge the greenhouse that causes and make global warming rise with per 10 years 0.2 degree centigrade speed, greenhouse gases have become topmost climatic shift factor, and the Global Ecological balance is constituted a serious threat.
Over nearly 20 years, some developed countries adopt various physics, chemistry and biological method research CO
2Collect, concentrate and fixing and conversion.Wherein, from the angle of sustainable development, biological CO
2Technique for fixing is a main and the most effective solid carbon mode on the earth, plays a decisive role in carbon cycle.Little algae is owing to have the photosynthetic rate height, breeding is fast, environmental compatibility is strong, processing efficiency is high and easily and advantage such as other engineering is integrated, is widely used in CO in the waste gas
2Capture.Little algae fixation of C O
2The potential Application Areas of another of technology is an enclosed space, as CO in submarine and the manned spacecraft
2Removal.The biological CO that captures of little algae
2The best of the processing of greenhouse gases, continuable method have been become.
And the petrochemical industry resource a large amount of uses, when bringing Greenhouse effect, climatic deterioration, the scarcity of petrochemical industry resource also makes the in short supply in a large number of diesel oil.In order to tackle present diesel oil resource shortage, biofuel has become international focus as the substitute of diesel oil.China's biofuel industry is day by day risen, and China's biofuel at present main be feedstock production still with oil plants such as oil crops such as soybean and Semen Brassicae campestris, oil palm and manioca wood fruit and animal grease, useless food and drink wet goods, though the raw material sources of biofuel are more various, but various raw materials are very limited, also are difficult to satisfy human demand to fuel oil even if they are added up.The little algae of produce oil promptly can be converted into carbon sources such as carbonic acid gas, carbohydrate, hydrocarbon polymer and common grease a large amount of greases of storing in the frond under certain condition, and fat content surpasses the microalgae of biological total amount 20%.Little algae because of the oleaginousness height, be easy to cultivate, advantage such as yield per unit is big, and be regarded as a new generation or even the unique biodiesel raw material that can realize substituting fully petrifaction diesel.
Little algae fixation of C O
2Technology also rationally utilizes simultaneously the grease of its generation to become the focus that the whole world is studied and paid close attention to.On October 1st, 2007, international energy company (International Energy Inc) announces that exploitation is the new technology of raw material production biofuel with the little algae of green.Since 21 century, oil price rapidly rises violently, to the cry of environmental protection grow to even greater heights, new technical progress, and United States Government hammers at renewable clean fuel etc., reignited the enthusiasm that people develop algae bio fuel.2006, US Congress's Helios (TAIYANGSHEN) plan of 400,000,000 dollars of supports from sunlight to fuel of agreeing in principle to furnish funds for.The approach from sunlight to fuel two that the TAIYANGSHEN plan proposes is and adopts photosynthetic established law synthesized micro-organism fuel, promptly utilizes photosynthetic microorganism, directly produces fuel from sunlight.In the same year, the U.S. two tame enterprises have set up the commercial system that can be connected with 1040 megawatt power plant flue gas, successfully utilize the CO in the stack gas
2Carry out extensive light compositing and cultivate little algae, and little algae is converted into biology " crude oil "." fire fuel " company has started " miniature manhattan project ", and the task of this plan was to realize the industrialization of algae produce oil and the target of following production every day MMbbl bio-crude oil by 2010.2008, U.S. Department of Defense announces to drop into 2,000 ten thousand dollar pools and carries out the research work of little algae biofuel, main purpose was before 2010 to confirm and makes based on the biomass fuel of marine alga can realize commercialization and become the substitute of JP-8 rocket engine fuel that this project is by each mechanism's common implementing that spreads all over the U.S..
Except that the U.S., the government in Australia, Japan, West Europe, India and South Africa or enterprise also drop into the research that huge fund is carried out little algae biofuel.Developed jointly out as Japanese two companies and to have utilized little algae CO
2Convert the new technology of alcohol fuel to, plan to develop relevant devices, and drop into suitability for industrialized production in 2010.Britain also started an algae bio fuel public funding project in 2008, and plan will be costed 2,600 ten thousand pounds, realized utilizing algae production and transport fuel to replace traditional fossil oil before the year two thousand twenty.In addition, Israel a company has externally showed in 2007 and utilizes marine alga to absorb CO
2, be the technology of biomass energy with conversion of solar energy, per 5 kilograms of algae can produce 1 liter of fuel.
At present, little algae biofuel price is higher, present relatively petrifaction diesel and utilize the biofuel of Vegetable oil lipoprotein production at all can't adapt to the demand of existing market.The deficiency of little algal biomass resource is little algae biofuel development " bottleneck ", and present little algae large scale culturing level is still limited, and this makes little algae biofuel hold at high price.And by little algae production of biodiesel process and CO
2The height coupling that reduces discharging is the effective means that reduces little algae biofuel cost.At present, the CO per ton of European Union
2The index price is about 20 Euros, 1 ton of CO of the every reduction of discharging of China
2The index price be about 100 yuans.The production of little algae biofuel realizes CO simultaneously
2Reduce discharging, can effectively reduce the cost of little algae biofuel.In addition, the CO that is coupled in little algae production of biodiesel process
2Reduction of discharging has more far-reaching social effect, can effectively remove greenhouse gases, and chlorella of the present invention can be good at being fit to the production and the CO of little algae biofuel
2The coupling that reduces discharging has been compared better CO with other little algaes
2Specific absorption and better fat content.
Summary of the invention
Technical problem to be solved by this invention provides a kind of chlorella, and the little algae of this strain can be used for the CO in the efficient captured gas of greasy while of production high yield
2, to solve the existing too high problem of microalgae grease production cost.
Another technical problem to be solved by this invention provides the application of above-mentioned chlorella.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
A kind of chlorella LICME001, its called after chlorella (Chlorella vulgaris) of classifying.Chlorella LICME001 of the present invention belongs to Chlorophyta Chlorococcale, chlorella section, general living chlorella.This bacterial strain is preserved in Chinese typical culture collection center (being called for short CCTCC) at present; Address: Wuhan, Wuhan University; Postcode: 430072; The numbering of registering on the books: CCTCC NO:M 209256; Preservation date: on November 5th, 2009.
Above-mentioned chlorella LICME001 is from marine site, Lianyun Harbour, Jiangsu Province, gets seawater, with the f/2 substratum of improvement, cultivates.Find to exist this chlorella with microscopic examination, the back obtains with the water droplet partition method.
CCTCC NO:M 209256 bacterial strains have following characteristics:
(1) colonial morphology: unicellular algae, Chang Dansheng also often has most cells to gather in together; It is spherical, oval that cell mostly is; 1 Zhousheng, cup-shaped or flaky chloroplast(id) are arranged in each cell, have 1 pyrenoids or lack as, 1 nucleus; Cell walls is very thin usually; Each cell can produce 2,4,8 or 16 autospores, carries out vegetative propagation.
(2) thalli morphology: green that nutrient solution is evenly distributed or deep green liquid.
(3) tolerance: this chlorella has stronger extreme environment tolerance, can tolerate pH5～10,21～30 ℃ and strong illumination.
The application of above-mentioned chlorella in producing microalgae grease.
Above-mentioned chlorella is at fixation of C O
2In application.
Above-mentioned chlorella is producing microalgae grease fixation of C O simultaneously
2In application.Concrete method is: at 23～27 ℃, pH 5～10, air flow 0.1～0.5vvm, CO wherein
2Content is under 3～24.5% (v/v), the illumination condition, and CCTCCNO:M 209256 was cultivated 10～20 days in the f/2 substratum of improvement.Preferable methods is: at 26～27 ℃, pH 7～9, air flow 0.15～0.3vvm, CO wherein
2Content is under 5～12% (v/v), the 5000lux illumination condition, and CCTCC NO:M 209256 was cultivated 15～20 days in the f/2 substratum of improvement.Most preferred method is: at 27 ℃, pH 7.2, air flow 0.25vvm, CO wherein
2Content is under 5～12% (v/v), the 5000lux illumination condition, and CCTCC NO:M209256 was cultivated 15 days in the f/2 substratum of improvement.
The f/2 substratum of mentioned improvement comprises following component among the present invention: the f/2 substratum for every 1L improvement comprises: 29.23g/L sodium-chlor, 105g/L Repone K, 11.09g/L magnesium sulfate heptahydrate, 1.21g/L Tris alkali, 1.83g/L two hydration calcium chloride, 0.25g/L sodium bicarbonate and 3.0mL trace substratum.
Wherein, the trace substratum for every 1L comprises: 281.3mg/L SODIUMNITRATE, 21.2mg/L sodium dihydrogen phosphate-water, 16.35mg/L Na
2-EDTA, 11.8mg/L six ferric sulfate hydrates, 675 μ g/L, 4 hydration Manganous chloride tetrahydrates, 37.5 μ g/L cobalt chloride hexahydrates, 37.5 μ g/L cupric sulfate pentahydrates, 82.5 μ g/L Zinc Sulphate Heptahydrates, 22.5 μ g/L Sodium orthomolybdates, 0.375mg/L vitamins B
1, 0.188 μ g/L vitamins B
12With 0.188 μ g/L vitamin H.【Guillard，R.R.L.1975.Culture?ofphytoplankton?for?feeding?marine?invertebrates.pp?26-60.In?Smith，W.L.and?Chanle，y?M.H.(eds.)Culture?of?Marine?Invertebrate?Animals.Plenum?Press，New?York，USA.】
Beneficial effect: chlorella LICME001 provided by the invention (Chlorella vulgaris LICME001) can tolerate the CO of 3～24.5% (v/v)
2, and can reach 12～55%CO
2Remove rate.Chlorella LICME001 provided by the invention (Chlorella vulgaris LICME001) can also absorb the CO in the gas on the greasy basis of high yield
2Can realize CO in the waste gas
2Reduction of discharging.Utilize this chlorella in optical-biological reaction, utilize suitable cultural method, can form biomass, fixation of C O fast
2, and the accumulation grease, fat content can reach 17～31%.The chlorella of invention has higher CO than common seawater algae
2Fixed efficiency, and can obtain the microalgae grease of high yield by selecting suitable culture condition, therefore can reduce little algae significantly and cultivate cost, for preparing biofuel, microalgae grease provide a strain better production bacterial classification.
Description of drawings
Fig. 1 is culture apparatus and the process flow diagram of chlorella of the present invention.
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand that the described concrete material proportion of embodiment, processing condition and result thereof only are used to illustrate the present invention, and should also can not limit the present invention described in detail in claims.
The used device of following examples as shown in Figure 1, CO
2Enter gas mixer through gas meter control respectively with air and mix, after gas filter filters, enter CO again
2Detector on-line monitoring CO
2Content, the inlet mouth by Gu Paoshi bioreactor glass tank base enters tank body and chlorella contact reacts, unabsorbed CO afterwards
2Enter another CO through the discharge of the air outlet at top again with air
2Detector on-line monitoring CO
2Content.Whole bubbling style bioreactor glass tank body can have heating rod and feed the cooling water control temperature, and can accept illumination.
Embodiment 1:
In the self-control bioreactor, inserting cell density is 5 * 10 with the f/2 substratum 5L that improves
7Individual/ml algae liquid 500mL.Feed the air of 0.25vvm, CO
2Content is 0.03% (v/v), cultivates after 15 days, and biomass reaches 1.1g/L, CO
2Specific absorption 6%.Fat content is 21% when cultivating 15 days.
Embodiment 2:
With the f/2 substratum 5L of improvement in the self-control bioreactor, initial pH7.2,27 ℃ ± 0.5 ℃ of temperature, light intensity 5000lux feeds 0.25vvm air and CO
2Mixed gas, wherein CO
2Content is 5% (v/v), inserts cell density and is about 5 * 10
7Individual/mL algae liquid 500mL.Cultivate after 15 days, biomass reaches 2.23g/L, CO
2Specific absorption be 51%.Fat content is 31% when cultivating 15 days.
Embodiment 3:
With the f/2 substratum 5L of improvement in the self-control bioreactor, initial pH7.2,27 ℃ ± 0.5 ℃ of temperature, light intensity 5000lux feeds 0.25vvm air and CO
2Mixed gas, wherein CO
2Content is 10% (v/v), inserts cell density and is about 5 * 10
7Individual/mL algae liquid 500mL.Cultivate after 15 days, biomass reaches 1.91g/L, CO
2Specific absorption be 26%.Fat content is 22% when cultivating 15 days.
Embodiment 4:
With the f/2 substratum 5L of improvement in the self-control bioreactor, initial pH7.2,27 ℃ ± 0.5 ℃ of temperature, light intensity 5000lux feeds 0.25vvm air CO
2Mixed gas, CO
2Content is 15%, inserts cell density and is about 5 * 10
7Individual/ml algae liquid 500ml.Cultivate after 15 days, biomass reaches 1.31g/L, CO
2Specific absorption be 22%.Fat content is 19% when cultivating 15 days.
Embodiment 5:
With the f/2 substratum 5L of improvement in the self-control bioreactor, initial p H7.2,27 ℃ ± 0.5 ℃ of temperature, light intensity 5000lux feeds 0.25vvm air CO
2Mixed gas, CO
2Content is 20%, inserts cell density and is about 5 * 10
7Individual/ml algae liquid 500ml.Cultivate after 15 days, biomass reaches 1.12g/L, CO
2Specific absorption be 17%.Fat content is 18.1% when cultivating 15 days.
Embodiment 6:
With the f/2 substratum 5L of improvement in the self-control bioreactor, initial p H7.2,27 ℃ ± 0.5 ℃ of temperature, light intensity 5000lux feeds 0.25vvm air CO
2Mixed gas, CO
2Content is 24.5%, inserts cell density and is about 5 * 10
7Individual/ml algae liquid 500ml.Cultivate after 15 days, biomass reaches 1.12g/L, CO
2Specific absorption be 13%.Fat content is 16.8% when cultivating 15 days.
Embodiment 7:
The character control experiment of the chlorella of buying on the Chlorella vulgaris LICME002 of this chlorella CCTCC NO:M 209256 and this laboratory preservation and the market (purchasing) in birds and flowers market, the Confucius Temple, Nanjing.With the f/2 substratum 5L of improvement in the self-control bioreactor, initial pH7.2,27 ℃ ± 0.5 ℃ of temperature, light intensity 5000lux feeds 0.25vvm and air CO
2Mixed gas, wherein CO
2Content is 5% (v/v), inserts cell density respectively and is about 5 * 10
7The chlorella of buying on the CCTCC NO:M 209256 of individual/ml algae liquid 500mL and chlorella LICME002 and the market.
CCTCC NO:M 209256 cultivates 15 days artifact amounts and reaches 2.23g/L, CO
2Specific absorption be 51%.Fat content is 31% when cultivating 15 days.
Chlorella LICME002 cultivates 15 days artifact amounts and reaches 1.22g/L, CO
2Specific absorption be 15%.Fat content is 18.1% when cultivating 15 days.
The chlorella of buying on the market is cultivated 15 days artifact amounts and reaches 0.63g/L, CO
2Specific absorption be 7.6%.Fat content is 10.1% when cultivating 15 days.
Claims (6)
1. a chlorella, its classification called after chlorella Chlorella vulgaris has been preserved in Chinese typical culture collection center, and its deposit number is CCTCC NO:M 209256.
2. the application of the described chlorella of claim 1 in producing microalgae grease.
3. the described chlorella of claim 1 is at fixation of C O
2In application.
4. the described chlorella of claim 1 is producing microalgae grease fixation of C O simultaneously
2In application.
5. application according to claim 4 is characterized in that at 23～27 ℃, pH 5～10, air flow 0.1～0.5vvm, CO wherein
2Content is under 3～24.5% (v/v), the illumination condition, and CCTCC NO:M 209256 was cultivated 10～20 days in the f/2 substratum of improvement.
6. application according to claim 5 is characterized in that at 26～27 ℃, pH 7～9, air flow 0.15～0.3vvm, CO wherein
2Content is under 5～12% (v/v), the 5000lux illumination condition, and CCTCC NO:M209256 was cultivated 15～20 days in the f/2 substratum of improvement.
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| CN201019026021XA CN101824386B (en) | 2010-02-04 | 2010-02-04 | Chlorella and application thereof |
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| CN101824386B CN101824386B (en) | 2011-12-21 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101967450A (en) * | 2010-11-12 | 2011-02-09 | 河南师范大学 | Method for cultivating chlorella |
| CN102586116A (en) * | 2011-01-14 | 2012-07-18 | 江南大学 | Common chlorella as well as culturing method and application thereof |
| WO2013013433A1 (en) * | 2011-07-22 | 2013-01-31 | 中国科学院烟台海岸带研究所 | Chlorella mutant strain and application thereof |
| CN104974931A (en) * | 2015-05-27 | 2015-10-14 | 上海理工大学 | Testing apparatus for CO2 in microalgae organism immobilization combustion flue gas |
| CN106399109A (en) * | 2016-11-07 | 2017-02-15 | 辽宁东科电力有限公司 | Chlorella vulgaris used for combined treatment of wastewater and waste gas while synchronously realizing solid carbon denitrification |
| CN106754387A (en) * | 2016-12-19 | 2017-05-31 | 山西大学 | A kind of three-dimensional corner algae culture medium |
| CN107541466A (en) * | 2017-08-02 | 2018-01-05 | 海南大学 | A kind of chlorella of resistance to strong light of salt tolerant high temperature resistant for discharging aroma type smell and its application |
| CN108977362A (en) * | 2017-06-05 | 2018-12-11 | 财团法人食品工业发展研究所 | The strain of Chlorococcum (CHLORELLA LEWINII) algae and its purposes |
| CN109576158A (en) * | 2017-09-28 | 2019-04-05 | 中国石油化工股份有限公司 | The grease-contained chlorella of one plant of richness and its culture application |
-
2010
- 2010-02-04 CN CN201019026021XA patent/CN101824386B/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101967450B (en) * | 2010-11-12 | 2012-07-04 | 河南师范大学 | Method for cultivating chlorella |
| CN101967450A (en) * | 2010-11-12 | 2011-02-09 | 河南师范大学 | Method for cultivating chlorella |
| CN102586116A (en) * | 2011-01-14 | 2012-07-18 | 江南大学 | Common chlorella as well as culturing method and application thereof |
| WO2013013433A1 (en) * | 2011-07-22 | 2013-01-31 | 中国科学院烟台海岸带研究所 | Chlorella mutant strain and application thereof |
| CN104974931A (en) * | 2015-05-27 | 2015-10-14 | 上海理工大学 | Testing apparatus for CO2 in microalgae organism immobilization combustion flue gas |
| CN106399109B (en) * | 2016-11-07 | 2020-08-07 | 辽宁东科电力有限公司 | Chlorella strain for synchronously realizing carbon fixation and denitration by combined treatment of wastewater and waste gas |
| CN106399109A (en) * | 2016-11-07 | 2017-02-15 | 辽宁东科电力有限公司 | Chlorella vulgaris used for combined treatment of wastewater and waste gas while synchronously realizing solid carbon denitrification |
| CN106754387A (en) * | 2016-12-19 | 2017-05-31 | 山西大学 | A kind of three-dimensional corner algae culture medium |
| CN108977362A (en) * | 2017-06-05 | 2018-12-11 | 财团法人食品工业发展研究所 | The strain of Chlorococcum (CHLORELLA LEWINII) algae and its purposes |
| CN107541466B (en) * | 2017-08-02 | 2019-07-05 | 海南大学 | It is a kind of discharge aroma type smell the resistance to strong light of salt tolerant high temperature resistant chlorella and its application |
| CN107541466A (en) * | 2017-08-02 | 2018-01-05 | 海南大学 | A kind of chlorella of resistance to strong light of salt tolerant high temperature resistant for discharging aroma type smell and its application |
| CN109576158A (en) * | 2017-09-28 | 2019-04-05 | 中国石油化工股份有限公司 | The grease-contained chlorella of one plant of richness and its culture application |
| CN109576158B (en) * | 2017-09-28 | 2022-02-08 | 中国石油化工股份有限公司 | Oil-rich chlorella and culture application thereof |
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