CN101824083B - C-terminal deletion type p43 protein and application thereof in medicaments for treating tumor - Google Patents
C-terminal deletion type p43 protein and application thereof in medicaments for treating tumor Download PDFInfo
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Abstract
The invention relates to a deletion type recombinant human p43 protein and application thereof in medicaments for treating tumor. Through constructing the deletion type p43 protein, a structure domain for specifically inhibiting the generation of neo-vascularization can be determined so as to reduce the side effect. In addition, the molecular weight of the deletion type p43 protein is smaller, thus the deletion type p43 protein is easy to absorb and has better curative effect.
Description
Technical field
The present invention relates to absence type recombinant human p 43 protein and the application in anti-tumor medicine thereof.
Background technology
Malignant tumour is the formidable enemy who threatens human health, and it is main method that medical circle mainly takes to kill cancer cells to the treatment of malignant tumour, that is: operation, chemotherapy, radiotherapy etc.These methods also injuring normal cell when killing cancer cells, and easily make tumour cell produce resistance.Thereby people are exploring and a kind ofly can kill in specific manner tumour cell and injuring normal cell not, are difficult for again making tumour to produce the method for cancer of resistance simultaneously always.The new drug that is used for the treatment of at present tumour emerges in an endless stream.In recent years, along with the fast development of Protocols in Molecular Biology, people pay close attention to the method for using genetically engineered drug treatment malignant tumour gradually.Traditional chemotherapy of tumors is used cytotoxic drug mostly, when killing tumour cell, also normal cell is caused damage, and weakens people's physique.So a lot of Advanced Carcinoma Patient are much more last, because cannot bear, treat and lethal dying.Malignant tumour has a common characteristic, that be exactly local cell insanely, do not breed with being contained, and maintain this propagation, just mean a large amount of nutrition supplies of needs.In the swollen neoplastic while, the inside of knurl body and can form the blood vessel of a large amount of supply nutrients around.If can cut off so these, support " pipeline ", the source of nutrition of blocking-up tumour, tumour also will die of exhaustion.
The new vessel of current rise suppresses therapy and has brought dawn for thoroughly treating tumour.The particularly a large amount of nutrition supply of growth needs of solid tumor of tumour is found in research, in tumour, can form a large amount of new vesseles around.Anti-angiogenic therapy is mainly by suppressing the growth of the vascular endothelial cell of rapid abnormal propagation, specificity suppresses tumor vascular endothelial cell propagation, thereby reach prevention tumor-blood-vessel growth, make tumour capillary vessel generation atrophy, cut off the nutrition supply of tumour, make apoptosis of tumor cells or degenerate to initial dormant state, thereby reach the object for the treatment of cancer.
Through the continuous effort of nearly ten years, found the multiple neovascularization inhibitor that promises to be anti-tumor medicine now.Wherein the most representative is human Endostatin (human endostatin).Relevant human Endostatin (Endostatin) in the world first report be the paper of the mouse endostatin gene of the research group of U.S. Harvard Medical School Folkman (Folkman) that publishes on Cell magazine in January, 1997, this research finds that Angiostatin and Endostatin can eliminate malignant tumour in Mice Body, and does not recur again.Thereby these two kinds of medicines are to stop for tumour provides the growth of the blood vessel of nutrition to treat tumour.Caused at that time international sensation, Endostatin becomes rapidly the study hotspot of therapeutic field of tumor.U.S. FDA gives special approval to that in the situation that not completing preclinical study 30 examples mix genetically engineered injection with human Endostatin (Angiostatin) and mouse Endostatin (Endostatin) to the unresponsive patients with advanced cancer of other medicines and carry out clinical trial at the beginning of 99.。At present, it is clinical that Endostatin has passed through the first phase of FDA, is at present the second stage of clinical stage.But because the solvability of Endostatin is poor, its preparation cost is raise, also can only adopt liquid drugs injection formulation in the use, this is limited by very large its application simultaneously.
P43 albumen is the cofactor of mammal aminoacyl-tRNA synthetase, directly regulates on the one hand endotheliocyte to form the physiological process of capillary vessel, suppresses tumor vascular generation on the other hand by changing microenvironment.The anti-angiogenesis activity of p43 albumen and suppress tumor growth effect in vitro and experimentation on animals obtain and confirm.People's gene restructuring p43 albumen demonstrates the potentiality that it is developed to novel therapeutic cancer drug, resists multiple primary and transitivity noumenal tumour is all effective, and can with chemotherapy and radiation Synergistic treatment.
P43 albumen is as the family member of human body aminoacyl-tRNA synthetase system and be that endothelial mononuclear cell activates propeptide and found by people such as Sophie Q in 1997.P43 albumen is single chain protein, 312 amino acid of total length, and secondary structure contains 11 β lamellas.Korea S Imagene company (WO01/95927) conducts in-depth research the structure of human p 43 protein, biological activity, result shows that human p 43 protein can suppress the growth of the vascular endothelial cell of rapid abnormal propagation, and suppressing the generation of chick chorioallantoic membrane new vessel, this explanation p43 albumen has potential antitumor action.
But, still need at present p43 albumen further to improve, obtain the better medicine of antitumous effect.
Summary of the invention
The present inventor finds through large quantity research, 312 of p43 albumen amino acid whose parts is excised to the deletion type p 43 protein obtaining and have higher anti-tumor activity.
Therefore, an object of the present invention is to provide deletion type p 43 protein, it has higher anti-tumor activity.
Another object of the present invention is to provide the purposes of deletion type p 43 protein in treatment antitumor drug.
Of the present invention to also have an object to be to provide a kind of for antitumor medicine composition, and it comprises deletion type p 43 protein.
The invention provides a kind of deletion type p 43 protein, it comprises shown in SEQ ID NO:1 and lacks the resulting albumen of 250-312 amino acids in aminoacid sequence.
In a preferred embodiment of the present invention, described deletion type p 43 protein comprises shown in SEQ ID NO:1 and lacks the resulting albumen of 265-312 amino acids in aminoacid sequence.
In a preferred embodiment of the present invention, described deletion type p 43 protein comprises shown in SEQ ID NO:1 and lacks the resulting albumen of 265-289 amino acids in aminoacid sequence.
In a preferred embodiment of the present invention, described deletion type p 43 protein comprises the albumen shown in following:
(1) SEQ ID NO:2 sequence
(2) SEQ ID NO:3 sequence
(3) SEQ ID NO:4 sequence
(4) SEQ ID NO:5 sequence
(5) SEQ ID NO:6 sequence.
In a preferred embodiment of the present invention, the equimolecular coupling of described deletion type p 43 protein and BSA, PEG and/or human serum albumin, forms polypeptide conjugate.
In a preferred embodiment of the present invention, described conjugate consists of polypeptide, linking agent and BSA, wherein said linking agent glutaraldehyde or EDAC.
The present invention also provides a kind of antitumor medicine composition, the deletion type p 43 protein of the present invention that it comprises (a) safe and effective amount, conjugate or its combination; And (b) pharmaceutically acceptable carrier or vehicle.
The present invention also provides the purposes of deletion type p 43 protein of the present invention in preparing medicine for treating tumor thing.
The present invention also provides the nucleotide sequence of the deletion type p 43 protein of the present invention of encoding.
By building deletion type p 43 protein, thereby can determine that specificity suppresses the structural domain minimizing side effect that new vessel generates, the molecular weight of deletion type p 43 protein is less in addition, thereby is easy to absorb, have higher curative effect.
Accompanying drawing explanation
Fig. 1 represents the pcr amplification result of p43 series deletant object segment, and wherein 1 represents p43-6; 2 represent p43-7; 3 represent p43-8; 4 represent p43-9; 5 represent p43-10; 6 represent DL2000.
Fig. 2 A represents the result of target protein induction, and wherein 1,12 represent protein molecular weight standard; 2 represent before p43-6 induction; 3 represent the result after p43-6 induction; 4 represent before p43-7 induction; 5 represent the result after p43-7 induction; 6 represent before p43-8 induction; 7 represent the result after p43-8 induction; 8 represent before p43-9 induction; 9 represent the result after p43-9 induction; 10 represent before p43-10 induction; 11 represent the result after p43-10 induction.
Fig. 2 B represents expression-form analysis, and wherein 1 represents the ultrasonic rear supernatant of p43-6; 2 represent the ultrasonic postprecipitation of p43-6; 3 represent the ultrasonic rear supernatant of p43-7; 4 represent the ultrasonic postprecipitation of p43-7; 5 represent protein molecular weight standard; 6 represent the ultrasonic rear supernatant of p43-8; 7 represent the ultrasonic postprecipitation of p43-8; 8 represent the ultrasonic rear supernatant of p43-9; 9 represent the ultrasonic postprecipitation of p43-9; 10 represent the ultrasonic rear supernatant of p43-10; 11 represent the ultrasonic postprecipitation of p43-10.
Fig. 3 represents p43 series deletant purifying collection of illustrative plates, the wherein purification result of 1 expression p43-6; 2 represent the purification result of p43-7; 3 represent the purification result of p43-8; 4 represent the purification result of p43-9; 5 represent the purification result of p43-10.
Fig. 4 represents p43 series deletant purifying schema.
Fig. 5 represents the effect that p43 deletant forms HUVEC tube chamber, and wherein 1 represents negative control; 2 represent p43 total length; 3 represent p43-6; 4 represent p43-7; 5 represent p43-8; 6 represent p43-9; 7 represent p43-10.
Fig. 6 represents that p43 deletant is to HUVEC inhibition of metastasis result, wherein the comparison schematic diagram of 1 expression p43 deletant and full-length proteins inhibition of metastasis activity; 2 represent that p43 deletant is with respect to the active schematic diagram of inhibition of full-length proteins.
embodiment
The compound that the present invention that the pharmaceutical composition the present invention relates to comprises safe and effective amount relates to and pharmaceutically acceptable carrier." safe and effective amount " means that the consumption of compound is enough to improve feelings to be cured the disease in the medical science of generally approval is judged category, and do not occur severe side effect during treatment.The safe and effective amount of certain compound should be determined according to concrete feelings to be cured the disease, the patient's that receives treatment age and physiological situation, coincident with severity degree of condition, the course for the treatment of factor such as length, pharmaceutical carrier and route of administration.Now in composition, comprise approximately 0.1% to approximately 99.9% compound by weight.
In the present invention, if not special explanation, percentage ratio (%) or part all refer to weight percentage or the weight part with respect to pure resin.
In the present invention, if not special explanation, each related component or its preferred ingredient can be combined to form new technical scheme mutually.
In the present invention, if not special explanation, all embodiments mentioned in this article and preferred implementation can be combined to form new technical scheme mutually.
In the present invention, if there is no contrary explanation, in composition, the content sum of each component is 100%.
In the present invention, if there is no contrary explanation, in composition, the umber sum of each component can be 100 weight parts.
In the present invention, unless there are other explanations, numerical range " a-b " represents that the breviary that a closes to the arbitrary real array between b represents, wherein a and b are real numbers.For example numerical range " 0-5 " represents all to have listed the whole real numbers between " 0-5 " herein, and " 0-5 " just the breviary of these combinations of values represents.
In the present invention, unless there are other explanations, integer numerical range " a-b " represents that a represents to the breviary of the arbitrary integer combination between b, and wherein a and b are integers.For example integer numerical range " 1-N " represents 1,2 ... N, wherein N is integer.
In the present invention, unless there are other explanations, " its combination " represents the multicomponent mixture of described each element, for example two kinds, three kinds, four kinds and until the multicomponent mixture of maximum possible.
If do not particularly not pointed out, this specification sheets term " a kind of " used refers to " at least one ".
" scope " disclosed herein is with the form of lower limit and the upper limit.Can be respectively one or more lower limits, and one or more upper limit.Given range limits by a selected lower limit and a upper limit.Selected lower limit and the upper limit define the border of special scope.All scopes that can limit by this way comprise with capable of being combined, and any lower limit can be combined to form a scope with any upper limit.For example, for special parameter, listed the scope of 60-120 and 80-110, be interpreted as that the scope of 60-110 and 80-120 also expects.In addition, if the minimum extent value 1 and 2 of listing, and if listed maximum range value 3,4 and 5, scope below can all expect: 1-3,1-4,1-5,2-3,2-4 and 2-5.
In this article, except as otherwise noted, the ratio of each component or weight all refer to dry weight.
In this article, except as otherwise noted, the aminoacid sequence of p43 albumen is shown in SEQ ID NO:1.
In this article, except as otherwise noted, deletion type p 43 protein represents albumen or its examples of conservative variations shown in the SEQ ID NO:1 of brachymemma.
In this article, except as otherwise noted, lack N amino acids and represent to lack N-312 amino acids in SEQ ID NO:1 aminoacid sequence, wherein N represents the integer of 1-312.For example, lacking 300-312 amino acids represents to lack respectively 300-312 amino acids, 301-312 amino acids in SEQ ID NO:1 aminoacid sequence ... the combination of the aminoacid sequence of 311-312 amino acids and the 312nd amino acids gained.
One aspect of the present invention provides a kind of deletion type p 43 protein, and it comprises shown in SEQ ID NO:1 and lacks the resulting albumen of 250-312 amino acids in aminoacid sequence.
In a preferred embodiment of the present invention, described deletion type p 43 protein comprises shown in SEQ ID NO:1 and lacks the resulting albumen of 265-312 amino acids in aminoacid sequence.
In another preferred embodiment of the present invention, described deletion type p 43 protein comprises shown in SEQ ID NO:1 and lacks the resulting albumen of 265-289 amino acids in aminoacid sequence.
Of the present invention, also have in a preferred embodiment, described deletion type p 43 protein comprises the albumen shown in following:
(1) SEQ ID NO:2 sequence (p43-6)
(2) SEQ ID NO:3 sequence (p43-7)
(3) SEQ ID NO:4 sequence (p43-8)
(4) SEQ ID NO:5 sequence (p43-9)
(5) SEQ ID NO:6 sequence (p43-10).
In the present invention, described p43 albumen is conventional, discloses its preparation method and sequence in prior art, and specifically referring to US5641867 and WO0195927, all the elements of above-mentioned patent documentation are inserted in this by reference in full.In a preferred embodiment of the present invention, described p43 albumen is provided by Xinyi Pharmaceutical Factory.
In the present invention, the method for described formation deletion type p 43 protein is conventional, and those of ordinary skill in the art can directly obtain described deletion type p 43 protein according to description of the invention.In the art, the method for described formation deletion type p 43 protein generally comprises host expresses method, direct synthesis method and full length protein cutting method etc.
In the present invention, described deletion type p 43 protein also can be used for the equimolecular coupling with for example BSA, PEG and/or human serum albumin, thereby forms polypeptide conjugate.Conventionally, described conjugate consists of polypeptide, linking agent and BSA, and wherein said linking agent is preferably glutaraldehyde or EDAC.
In addition, deletion type p 43 protein of the present invention and conjugate thereof also can be used for preparing antitumor medicine composition.
Therefore, the present invention provides a kind of antitumor medicine composition on the other hand, the deletion type p 43 protein of the present invention that it comprises (a) safe and effective amount, conjugate or its combination; And (b) pharmaceutically acceptable carrier or vehicle.The quantity of deletion type p 43 protein of the present invention is generally 10 micrograms to 100 milligram/agent, preferably 100 micrograms to 50 milligram/agent, and better 1000 micrograms to 10 milligram/agent, best 3000 to 5000 micrograms/agent.
Term used herein " significant quantity " refers to therapeutical agent treatment, alleviates or prevent the amount of target disease or situation, or shows the amount of detectable treatment or preventive effect.For the accurate significant quantity of a certain object, depend on the nature and extent of the build of this object and healthy state, disease and the therapeutical agent selecting to give and/or the combination of therapeutical agent.
For the purposes of the present invention, effectively dosage is for giving individual approximately 1 microgram to 100 mg/kg body weight/day, and preferably 100 micrograms are to 50 mg/kg body weight/day albumen of the present invention.
Pharmaceutical composition also can contain pharmaceutically acceptable carrier.Term " pharmaceutically acceptable carrier " refers to be used for the treatment of the carrier of agent administration.This term refers to some such medicament carriers: they itself are not induced and produce accepting the harmful antibody of individuality of said composition, and after administration, there is no undue toxicity.These carriers are well known to those of ordinary skill in the art, in Remington ' s Pharmaceutical Sciences (Mark Pub.Co., N.J.1991), can find discussing fully about pharmaceutically acceptable vehicle (carrier).This class carrier includes, but is not limited to: salt solution, damping fluid, glucose, water, glycerine, ethanol, adjuvant and combination thereof.
In treatment or prophylactic compositions Chinese materia medica, acceptable carrier can contain liquid, as water, salt solution, glycerine and ethanol.In addition, in these carriers, also may there is complementary material, as wetting agent or emulsifying agent, pH buffer substance etc.
Conventionally, treatment or prophylactic compositions can be made to injectable dosage formulations, for example liquor or suspension, also can be made into before injection, be applicable to sneaking in solution or suspension, the solid form of liquid vehicle.
Once be made into composition of the present invention, can directly give object by it.Wait that the object that prevents or treat can be animal, especially people.
Treatment or prophylactic medicament containing polypeptide of the present invention of the present invention, can oral administration, the mode such as subcutaneous, intracutaneous, intravenous injection, intramuscularly applies.Therapeutic dose scheme can be single dose scheme or multi-agent scheme.
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment are only not used in and limit the scope of the invention for the present invention is described.The experimental technique of unreceipted actual conditions in the following example, condition routinely, or the condition of advising according to manufacturer conventionally.
Embodiment
embodiment 1: the deletion type p 43 protein (p43-6) of preparation SEQ ID NO:2 aminoacid sequence
The pcr amplification of object segment
The expression plasmid of disclosed p43 albumen in Chinese patent application CN101225371A of take is masterplate, with the primer sets shown in table 1 (primer is synthesized by Shanghai Jierui Biology Engineering Co., Ltd), adopt KOD-plus (TOYOBO) the reaction system nucleotide sequence (table 2) of pcr amplification SEQ ID NO:7 respectively, 1% agarose gel electrophoresis result shows, increases and has obtained size and expected the object band (Fig. 1) being consistent.
Table 1
Table 2
The structure of expression vector
PCR product is separated through agarose gel electrophoresis, rubber tapping is reclaimed, purifying; Utilize EcoR I and Xho I (TaKaRa) each PCR product of double digestion and pTIG-Trx plasmid vector respectively.With 4 ℃ of connections of T4 DNA (TaKaRa) ligase enzyme, spend the night, connect product Transformed E .coli TOP10 competent cell (TIANGEN Biotech (Beijing) Co., Ltd.).
Screening has the positive colony that foreign DNA inserts.Through ABI 3700 type sequenators, employing T7promoter and the two-way order-checking of T7 terminator primer, result proof has obtained the goal gene of SEQ ID NO:7 nucleotide sequence.
Transform respectively pTIG-Trx-p43 series deletant to E.coli BL21 (DE) competent cell (TIANGEN Biotech (Beijing) Co., Ltd.), through a small amount of cultivation, plasmid extraction and EcoR I and Xho I double digestion, identify to there is single bacterium colony that correct goal gene inserts, enlarged culturing, sets up the engineering bacteria with SEQ ID NO:7 nucleotide sequence expression vector.
The engineering bacteria building is inoculated in liquid LB, and 200rpm, 37 ℃ are cultured to 0.D.
600for 0.4-0.6, adding final concentration is the IPTG of 1mM, and 4h is cultivated in 200rpm, 37 ℃ of continuation.Centrifugal collection thalline, then with the resuspended thalline of 20mM Tris (pH8.0), ultrasonicly splits bacterium, and SDS-PAGE analyzes expression-form (Fig. 2).
After enlarged culturing, collect the thalline obtaining, add ultrasonication thalline under 1mM PMSF condition of ice bath, 4 ℃ of centrifugal 30min of 9000rpm, collect supernatant.With HisTrap FF affinity column, target protein is carried out to purifying, collect elutriant, lyophilize is concentrated, obtains the deletion type p 43 protein (Fig. 3) of SEQ ID NO:2 aminoacid sequence, and concrete purifying flow process is as Fig. 4.
embodiment 2: the deletion type p 43 protein (p43-7) of preparation SEQ ID NO:3 aminoacid sequence
Method as identical in embodiment 1 is prepared the deletion type p 43 protein of SEQ ID NO:3 aminoacid sequence, and different is the goal gene with the synthetic SEQ ID NO:8 nucleotide sequence of the corresponding primer shown in table 2.
embodiment 3: the deletion type p 43 protein (p43-8) of preparation SEQ ID NO:4 aminoacid sequence
Method as identical in embodiment 1 is prepared the deletion type p 43 protein of SEQ ID NO:4 aminoacid sequence, and different is the goal gene with the synthetic SEQ ID NO:9 nucleotide sequence of the corresponding primer shown in table 2.
embodiment 4: the deletion type p 43 protein (p43-9) of preparation SEQ ID NO:5 aminoacid sequence
Method as identical in embodiment 1 is prepared the deletion type p 43 protein of SEQ ID NO:5 aminoacid sequence, and different is the goal gene with the synthetic SEQ ID NO:10 nucleotide sequence of the corresponding primer shown in table 2.
embodiment 5: the deletion type p 43 protein (p43-10) of preparation SEQ ID NO:6 aminoacid sequence
Method as identical in embodiment 1 is prepared the deletion type p 43 protein of SEQ ID NO:6 aminoacid sequence, and different is the goal gene with the synthetic SEQ ID NO:11 nucleotide sequence of the corresponding primer shown in table 2.
embodiment 6:p43 series deletant albumen suppresses tumor angiogenesis experiment
Materials and methods: M199, FBS and pancreatin are purchased from Gibico; Transwell is purchased from corning; Matrigel is purchased from BD Biosciences; Fibronectin is purchased from Sigma; Fluorescence dye Calcein AM is purchased from Biotium.
Cell cultures: human umbilical vein endothelial (Human Umbilical Vein EndothelialCells, HUVEC) cell is so kind as to give by China Medicine University's drug screening center associate professor Yuan Shengtao.Cell cultures is in containing in the M199 substratum of 10%FBS, 37 ℃, 5%CO
2in incubator, cultivate.Until cell, test during in exponential phase of growth.
Endotheliocyte tube chamber forms and suppresses experiment
Matrigel glue, after melting on ice, adds in 96 orifice plates of precooling (60 μl/ hole), and 37 ℃ of placements solidify it in 30 minutes.Every hole adds the HUVEC (μl/ hole, 3.0 * 10 cell/100) with the M199 nutrient solution dilution containing 10%FBS.Add respectively a series of final concentrations is p43 total length and the deletant sample of 50 μ mol/ml simultaneously, hatches 4h for 37 ℃, adds fluorescence dye Calcein AM dyeing, fluorescence microscopy Microscopic observation, random Xuan Sange take pictures in the visual field (* 100).
Experimental result shows: p43-6, p43-7, p43-8, p43-9 and p43-10 absence type albumen suppress the activity of vascular endothelial cell tube chamber formation apparently higher than p43 albumen.The tube chamber of p43 albumen effect does not also rupture completely, has than more complete luminal structure, and the tube chamber of p43-6, p43-7, p43-8, p43-9 and the effect of p43-10 absence type albumen ruptures nearly all (Fig. 5).
Endothelial cell migration suppresses experiment
The cell migration of usining experiment is as the quantitative detecting method of p43 deletant activity, and inhibition of metastasis rate is as the judgement criteria of activity height.The inhibition of metastasis rate of deletant albumen and total length human p 43 protein are compared.HUVEC is through serum starvation 4-5h.In the lower chamber of Transwell, face is coated Fibronectin, puts in super clean bench air-dry.Trypsin digestion cell, uses PBS rinse,, counting resuspended with the M199 substratum containing 0.2%FBS.Obtained cell suspension 180 μ l add interior chamber (105 cells).Mistress adds the M199 substratum of 10%FBS, 540 μl/ holes.To in p43 total length and deletant Yu Nei chamber, add 20 μ l, in mistress, add 60 μ l (final concentration is 50 μ mol/ml), mix, and using PBS as negative control.Culture plate is placed in to 37 ℃, 5%CO
2in incubator, cultivate 16-18h.Discard nutrient solution in hole, with 90% ethanol fixed cell 10min.With cotton swab, wipe gently the cell that does not move on Nei Shi upper strata, 0.1% Viola crystallina room temperature dyeing 10min, rinses excess dyestuff with PBS, with 10% acetic acid 10 μl/ hole extracting 10min.With microplate reader, at 595nm wavelength, measure photoabsorption.According to following formula computation migration inhibiting rate: inhibition of metastasis rate=(A
contrast-A
process)/A
contrast* 100%.
Under the same conditions, the inhibition of metastasis activity of the vascular endothelial cell of p43-6, p43-7, p43-8, p43-9 and p43-10 absence type albumen is apparently higher than the p43 albumen (table 3) of total length.
Table 3, p43 deletant are to HMEC-1 inhibition of metastasis result
A
Sequence table
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Pro Pro Asn Gly Ser Val Pro Gly Asp Arg Ile Thr Phe Asp Ala Phe
245 250 255
Pro Gly Glu Pro Asp Lys Glu Leu Asn Pro Lys Lys Lys Ile Trp Glu
260 265 270
Gln Ile Gln Pro Asp Leu His Thr Asn Asp Glu Cys Val
275 280 285
<210>4
<211>278
<212>PRT
<213>p43-3 albumen
<400>4
Met Ala Asn Asn Asp Ala Val Leu Lys Arg Leu Glu Gln Lys Gly Ala
1 5 10 15
Glu Ala Asp Gln Ile Ile Glu Tyr Leu Lys Gln Gln Val Ser Leu Leu
20 25 30
Lys Glu Lys Ala Ile Leu Gln Ala Thr Leu Arg Glu Glu Lys Lys Leu
35 40 45
Arg Val Glu Asn Ala Lys Leu Lys Lys Glu Ile Glu Glu Leu Lys Gln
50 55 60
Glu Leu Ile Gln Ala Glu Ile Gln Asn Gly Val Lys Gln Ile Ala Phe
65 70 75 80
Pro Ser Gly Thr Pro Leu His Ala Asn Ser Met Val Ser Glu Asn Val
85 90 95
Ile Gln Ser Thr Ala Val Thr Thr Val Ser Ser Gly Thr Lys Glu Gln
100 105 110
Ile Lys Gly Gly Thr Gly Asp Glu Lys Lys Ala Lys Glu Lys Ile Glu
115 120 125
Lys Lys Gly Glu Lys Lys Glu Lys Lys Gln Gln Ser Ile Ala Gly Ser
130 135 140
Ala Asp Ser Lys Pro Ile Asp Val Ser Arg Leu Asp Leu Arg Ile Gly
145 150 155 160
Cys Ile Ile Thr Ala Arg Lys His Pro Asp Ala Asp Ser Leu Tyr Val
165 170 175
Glu Glu Val Asp Val Gly Glu Ile Ala Pro Arg Thr Val Val Ser Gly
180 185 190
Leu Val Asn His Val Pro Leu Glu Gln Met Gln Asn Arg Met Val Ile
195 200 205
Leu Leu Cys Asn Leu Lys Pro Ala Lys Met Arg Gly Val Leu Ser Gln
210 215 220
Ala Met Val Met Cys Ala Ser Ser Pro Glu Lys Ile Glu Ile Leu Ala
225 230 235 240
Pro Pro Asn Gly Ser Val Pro Gly Asp Arg Ile Thr Phe Asp Ala Phe
245 250 255
Pro Gly Glu Pro Asp Lys Glu Leu Asn Pro Lys Lys Lys Ile Trp Glu
260 265 270
Gln Ile Gln Pro Asp Leu
275
<210>5
<211>268
<212>PRT
<213>p43-4 albumen
<400>5
Met Ala Asn Asn Asp Ala Val Leu Lys Arg Leu Glu Gln Lys Gly Ala
1 5 10 15
Glu Ala Asp Gln Ile Ile Glu Tyr Leu Lys Gln Gln Val Ser Leu Leu
20 25 30
Lys Glu Lys Ala Ile Leu Gln Ala Thr Leu Arg Glu Glu Lys Lys Leu
35 40 45
Arg Val Glu Asn Ala Lys Leu Lys Lys Glu Ile Glu Glu Leu Lys Gln
50 55 60
Glu Leu Ile Gln Ala Glu Ile Gln Asn Gly Val Lys Gln Ile Ala Phe
65 70 75 80
Pro Ser Gly Thr Pro Leu His Ala Asn Ser Met Val Ser Glu Asn Val
85 90 95
Ile Gln Ser Thr Ala Val Thr Thr Val Ser Ser Gly Thr Lys Glu Gln
100 105 110
Ile Lys Gly Gly Thr Gly Asp Glu Lys Lys Ala Lys Glu Lys Ile Glu
115 120 125
Lys Lys Gly Glu Lys Lys Glu Lys Lys Gln Gln Ser Ile Ala Gly Ser
130 135 140
Ala Asp Ser Lys Pro Ile Asp Val Ser Arg Leu Asp Leu Arg Ile Gly
145 150 155 160
Cys Ile Ile Thr Ala Arg Lys His Pro Asp Ala Asp Ser Leu Tyr Val
165 170 175
Glu Glu Val Asp Val Gly Glu Ile Ala Pro Arg Thr Val Val Ser Gly
180 185 190
Leu Val Asn His Val Pro Leu Glu Gln Met Gln Asn Arg Met Val Ile
195 200 205
Leu Leu Cys Asn Leu Lys Pro Ala Lys Met Arg Gly Val Leu Ser Gln
210 215 220
Ala Met Val Met Cys Ala Ser Ser Pro Glu Lys Ile Glu Ile Leu Ala
225 230 235 240
Pro Pro Asn Gly Ser Val Pro Gly Asp Arg Ile Thr Phe Asp Ala Phe
245 250 255
Pro Gly Glu Pro Asp Lys Glu Leu Asn Pro Lys Lys
260 265
<210>6
<211>264
<212>PRT
<213>p43-5 albumen
<400>6
Met Ala Asn Asn Asp Ala Val Leu Lys Arg Leu Glu Gln Lys Gly Ala
1 5 10 15
Glu Ala Asp Gln Ile Ile Glu Tyr Leu Lys Gln Gln Val Ser Leu Leu
20 25 30
Lys Glu Lys Ala Ile Leu Gln Ala Thr Leu Arg Glu Glu Lys Lys Leu
35 40 45
Arg Val Glu Asn Ala Lys Leu Lys Lys Glu Ile Glu Glu Leu Lys Gln
50 55 60
Glu Leu Ile Gln Ala Glu Ile Gln Asn Gly Val Lys Gln Ile Ala Phe
65 70 75 80
Pro Ser Gly Thr Pro Leu His Ala Asn Ser Met Val Ser Glu Asn Val
85 90 95
Ile Gln Ser Thr Ala Val Thr Thr Val Ser Ser Gly Thr Lys Glu Gln
100 105 110
Ile Lys Gly Gly Thr Gly Asp Glu Lys Lys Ala Lys Glu Lys Ile Glu
115 120 125
Lys Lys Gly Glu Lys Lys Glu Lys Lys Gln Gln Ser Ile Ala Gly Ser
130 135 140
Ala Asp Ser Lys Pro Ile Asp Val Ser Arg Leu Asp Leu Arg Ile Gly
145 150 155 160
Cys Ile Ile Thr Ala Arg Lys His Pro Asp Ala Asp Ser Leu Tyr Val
165 170 175
Glu Glu Val Asp Val Gly Glu Ile Ala Pro Arg Thr Val Val Ser Gly
180 185 190
Leu Val Asn His Val Pro Leu Glu Gln Met Gln Asn Arg Met Val Ile
195 200 205
Leu Leu Cys Asn Leu Lys Pro Ala Lys Met Arg Gly Val Leu Ser Gln
210 215 220
Ala Met Val Met Cys Ala Ser Ser Pro Glu Lys Ile Glu Ile Leu Ala
225 230 235 240
Pro Pro Asn Gly Ser Val Pro Gly Asp Arg Ile Thr Phe Asp Ala Phe
245 250 255
Pro Gly Glu Pro Asp Lys Glu Leu
260
<210>7
<211>864
<212>DNA
<213>p43-1 nucleotide sequence
<400>7
atggcaaata atgatgctgt tctgaagaga ctggagcaga agggtgcaga ggcagatcaa 60
atcattgaat atcttaagca gcaagtttct ctacttaagg agaaagcaat tttgcaggca 120
actttgaggg aagagaagaa acttcgagtt gaaaatgcta aactgaagaa agaaattgaa 180
gaactgaaac aagagctaat tcaggcagaa attcaaaatg gagtgaagca aatagcattt 240
ccatctggta ctccactgca cgctaattct atggtttctg aaaatgtgat acagtctaca 300
gcagtaacaa ccgtatcttc tggtaccaaa gaacagataa aaggaggaac aggagacgaa 360
aagaaagcga aagagaaaat tgaaaagaaa ggagagaaga aggagaaaaa acagcaatca 420
atagctggaa gtgccgactc taagccaata gatgtttccc gtctggatct tcgaattggt 480
tgcatcataa ctgctagaaa acaccctgat gcagattctt tgtatgtgga agaagtagat 540
gtcggagaaa tagccccaag gacagttgtc agtggcctgg tgaatcatgt tcctcttgaa 600
cagatgcaaa atcggatggt gattttactt tgtaacctga aacctgcaaa gatgagggga 660
gtattatctc aagcaatggt catgtgtgct agttcaccag agaaaattga aatcttggct 720
cctccaaatg ggtctgttcc tggagacaga attacttttg atgctttccc aggagagcct 780
gacaaggagc tgaatcctaa gaagaagatt tgggagcaga tccagcctga tcttcacact 840
aatgatgagt gtgtggctac atac 864
<210>8
<211>855
<212>DNA
<213>p43-2 nucleotide sequence
<400>8
atggcaaata atgatgctgt tctgaagaga ctggagcaga agggtgcaga ggcagatcaa 60
atcattgaat atcttaagca gcaagtttct ctacttaagg agaaagcaat tttgcaggca 120
actttgaggg aagagaagaa acttcgagtt gaaaatgcta aactgaagaa agaaattgaa 180
gaactgaaac aagagctaat tcaggcagaa attcaaaatg gagtgaagca aatagcattt 240
ccatctggta ctccactgca cgctaattct atggtttctg aaaatgtgat acagtctaca 300
gcagtaacaa ccgtatcttc tggtaccaaa gaacagataa aaggaggaac aggagacgaa 360
aagaaagcga aagagaaaat tgaaaagaaa ggagagaaga aggagaaaaa acagcaatca 420
atagctggaa gtgccgactc taagccaata gatgtttccc gtctggatct tcgaattggt 480
tgcatcataa ctgctagaaa acaccctgat gcagattctt tgtatgtgga agaagtagat 540
gtcggagaaa tagccccaag gacagttgtc agtggcctgg tgaatcatgt tcctcttgaa 600
cagatgcaaa atcggatggt gattttactt tgtaacctga aacctgcaaa gatgagggga 660
gtattatctc aagcaatggt catgtgtgct agttcaccag agaaaattga aatcttggct 720
cctccaaatg ggtctgttcc tggagacaga attacttttg atgctttccc aggagagcct 780
gacaaggagc tgaatcctaa gaagaagatt tgggagcaga tccagcctga tcttcacact 840
aatgatgagt gtgtg 855
<210>9
<211>834
<212>DNA
<213>p43-3 nucleotide sequence
<400>9
atggcaaata atgatgctgt tctgaagaga ctggagcaga agggtgcaga ggcagatcaa 60
atcattgaat atcttaagca gcaagtttct ctacttaagg agaaagcaat tttgcaggca 120
actttgaggg aagagaagaa acttcgagtt gaaaatgcta aactgaagaa agaaattgaa 180
gaactgaaac aagagctaat tcaggcagaa attcaaaatg gagtgaagca aatagcattt 240
ccatctggta ctccactgca cgctaattct atggtttctg aaaatgtgat acagtctaca 300
gcagtaacaa ccgtatcttc tggtaccaaa gaacagataa aaggaggaac aggagacgaa 360
aagaaagcga aagagaaaat tgaaaagaaa ggagagaaga aggagaaaaa acagcaatca 420
atagctggaa gtgccgactc taagccaata gatgtttccc gtctggatct tcgaattggt 480
tgcatcataa ctgctagaaa acaccctgat gcagattctt tgtatgtgga agaagtagat 540
gtcggagaaa tagccccaag gacagttgtc agtggcctgg tgaatcatgt tcctcttgaa 600
cagatgcaaa atcggatggt gattttactt tgtaacctga aacctgcaaa gatgagggga 660
gtattatctc aagcaatggt catgtgtgct agttcaccag agaaaattga aatcttggct 720
cctccaaatg ggtctgttcc tggagacaga attacttttg atgctttccc aggagagcct 780
gacaaggagc tgaatcctaa gaagaagatt tgggagcaga tccagcctga tctt 834
<210>10
<211>804
<212>DNA
<213>p43-4 nucleotide sequence
<400>10
atggcaaata atgatgctgt tctgaagaga ctggagcaga agggtgcaga ggcagatcaa 60
atcattgaat atcttaagca gcaagtttct ctacttaagg agaaagcaat tttgcaggca 120
actttgaggg aagagaagaa acttcgagtt gaaaatgcta aactgaagaa agaaattgaa 180
gaactgaaac aagagctaat tcaggcagaa attcaaaatg gagtgaagca aatagcattt 240
ccatctggta ctccactgca cgctaattct atggtttctg aaaatgtgat acagtctaca 300
gcagtaacaa ccgtatcttc tggtaccaaa gaacagataa aaggaggaac aggagacgaa 360
aagaaagcga aagagaaaat tgaaaagaaa ggagagaaga aggagaaaaa acagcaatca 420
atagctggaa gtgccgactc taagccaata gatgtttccc gtctggatct tcgaattggt 480
tgcatcataa ctgctagaaa acaccctgat gcagattctt tgtatgtgga agaagtagat 540
gtcggagaaa tagccccaag gacagttgtc agtggcctgg tgaatcatgt tcctcttgaa 600
cagatgcaaa atcggatggt gattttactt tgtaacctga aacctgcaaa gatgagggga 660
gtattatctc aagcaatggt catgtgtgct agttcaccag agaaaattga aatcttggct 720
cctccaaatg ggtctgttcc tggagacaga attacttttg atgctttccc aggagagcct 780
gacaaggagc tgaatcctaa gaag 804
<210>11
<211>792
<212>DNA
<213>p43-5 nucleotide sequence
<400>11
atggcaaata atgatgctgt tctgaagaga ctggagcaga agggtgcaga ggcagatcaa 60
atcattgaat atcttaagca gcaagtttct ctacttaagg agaaagcaat tttgcaggca 120
actttgaggg aagagaagaa acttcgagtt gaaaatgcta aactgaagaa agaaattgaa 180
gaactgaaac aagagctaat tcaggcagaa attcaaaatg gagtgaagca aatagcattt 240
ccatctggta ctccactgca cgctaattct atggtttctg aaaatgtgat acagtctaca 300
gcagtaacaa ccgtatcttc tggtaccaaa gaacagataa aaggaggaac aggagacgaa 360
aagaaagcga aagagaaaat tgaaaagaaa ggagagaaga aggagaaaaa acagcaatca 420
atagctggaa gtgccgactc taagccaata gatgtttccc gtctggatct tcgaattggt 480
tgcatcataa ctgctagaaa acaccctgat gcagattctt tgtatgtgga agaagtagat 540
gtcggagaaa tagccccaag gacagttgtc agtggcctgg tgaatcatgt tcctcttgaa 600
cagatgcaaa atcggatggt gattttactt tgtaacctga aacctgcaaa gatgagggga 660
gtattatctc aagcaatggt catgtgtgct agttcaccag agaaaattga aatcttggct 720
cctccaaatg ggtctgttcc tggagacaga attacttttg atgctttccc aggagagcct 780
gacaaggagc tg 792
Claims (4)
1. a deletion type p 43 protein, described deletion type p 43 protein is selected from the albumen shown in following sequence:
(1) SEQ ID NO:2 sequence
(2) SEQ ID NO:3 sequence
(3) SEQ ID NO:4 sequence
(4) SEQ ID NO:5 sequence
(5) SEQ ID NO:6 sequence.
2. a pharmaceutical composition, the deletion type p 43 protein claimed in claim 1 that it comprises (a) safe and effective amount; And (b) pharmaceutically acceptable carrier or vehicle.
Deletion type p 43 protein claimed in claim 1 preparation suppress that vascular endothelial cell tube chamber forms and the medicine of endothelial cell migration in purposes.
Coding deletion type p 43 protein claimed in claim 1 nucleotide sequence.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910047144.1A CN101824083B (en) | 2009-03-06 | 2009-03-06 | C-terminal deletion type p43 protein and application thereof in medicaments for treating tumor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910047144.1A CN101824083B (en) | 2009-03-06 | 2009-03-06 | C-terminal deletion type p43 protein and application thereof in medicaments for treating tumor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101824083A CN101824083A (en) | 2010-09-08 |
| CN101824083B true CN101824083B (en) | 2014-01-15 |
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| CN200910047144.1A Expired - Fee Related CN101824083B (en) | 2009-03-06 | 2009-03-06 | C-terminal deletion type p43 protein and application thereof in medicaments for treating tumor |
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| Country | Link |
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| CN (1) | CN101824083B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1496269A (en) * | 2003-01-15 | 2004-05-12 | 艾玛基因有限公司 | Medical composition containing P43 protein and taxol for curing cancer, curing method and use using the same thereof |
| CN101190329A (en) * | 2006-11-29 | 2008-06-04 | 信谊药厂 | Medicinal composition for regrouping human P43 protein and its application in medication |
| CN101225371A (en) * | 2007-01-18 | 2008-07-23 | 信谊药厂 | Preparation technology of recombinant human p43 protein |
| CN101370821A (en) * | 2006-01-23 | 2009-02-18 | 伊玛吉恩有限公司 | Novel peptide and uses thereof |
-
2009
- 2009-03-06 CN CN200910047144.1A patent/CN101824083B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1496269A (en) * | 2003-01-15 | 2004-05-12 | 艾玛基因有限公司 | Medical composition containing P43 protein and taxol for curing cancer, curing method and use using the same thereof |
| CN101370821A (en) * | 2006-01-23 | 2009-02-18 | 伊玛吉恩有限公司 | Novel peptide and uses thereof |
| CN101190329A (en) * | 2006-11-29 | 2008-06-04 | 信谊药厂 | Medicinal composition for regrouping human P43 protein and its application in medication |
| CN101225371A (en) * | 2007-01-18 | 2008-07-23 | 信谊药厂 | Preparation technology of recombinant human p43 protein |
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|---|---|
| CN101824083A (en) | 2010-09-08 |
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Address after: 201206 Pudong New Area new Jinqiao Road, Shanghai, No. 905 Patentee after: Shanghai Xinyi Pharmaceutical Co. Ltd.. Address before: 201206 No. 905 Jinqiao Road, Shanghai Patentee before: Shanghai Sine Pharmaceutical Co., Ltd. |
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