CN101819154A - Chromatography type triglyceride self-measuring system - Google Patents
Chromatography type triglyceride self-measuring system Download PDFInfo
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Abstract
The invention discloses a chromatography type triglyceride self-measuring system which comprises a shell, a set of solution-guiding test paper, reaction test paper and color-developing test paper arranged in a cavity of the shell, a solution system and a solution feeding mechanism enabling the solution system to be in contact with the solution-guiding test paper. The main component of a solution in the solution system is a peroxidase solution, and the solution system can be arranged inside or outside the cavity of the shell; the shell is provided with a blood-taking hole, a receiving pad is arranged in the shell, and the receiving pad is provided with two working positions, namely a blood-taking position and a chromatography position; lipoproteinesterase and a substrate are arranged in the receiving pad, and the substrate is selected from glycerol, glycerol trioleate, serum containing triglyceride or plasma containing triglyceride; and the reaction test paper is fixedly provided with lipoprotein lipase, phosphoglycerol oxidase, glycerokinase, adenosine triphosphate and water-soluble magnesium salt. The chromatography type triglyceride self-measuring system can realize instant measurement of triglyceride in blood.
Description
Technical field
The present invention relates to a kind of medical detecting Instrument, be specifically related to a kind of method of measuring the chromatography type triglyceride self-measuring system of content of triglyceride in the blood and using content of triglyceride in this self-measuring system mensuration blood.
Background technology
(Triglyceride TG) is the fat molecule that long-chain fatty acid and glycerine form to triglyceride.Triglyceride is the maximum lipid of people's in-vivo content, and most tissues all can be utilized triglyceride decomposition product energize, and tissues such as liver, fat can also carry out the synthetic of triglyceride simultaneously, store in adipose tissue.
The term of reference of triglyceride is:＜2.3mmol/L (＜200mg/dL).The height of normal person's triglyceride levels is influenced by living condition, and difference and interindividual variation and increased gradually with the age and to raise all greater than T-CHOL in it was individual.When TG＞4.5mmol/L (＞400mg/dL) can be diagnosed as hypertriglyceridemia.TG increase be mainly seen in that familial hypertriglyceridemia, familial combined hyperlipidaemia, coronary heart disease, atherosclerotic (AS), diabetes, nephrotic syndrome, first subtract, biliary tract infraction, glycogen storage disease, gestation, oral contraceptive, excessive drinking, acute pancreatitis (＞11.3mmol/L).
Serum triglyceride/triacylglycerol (TG) is an important clinical blood fat conventional determining index, particularly along with to the going deep into of its atherogenicity effect research, TG as one of coronary heart disease independently hazards come into one's own day by day.Mensuration serum TG level is mainly used in diagnosis and the application Friedewald formula understanding TG metabolism status in the body, hypertriglyceridemia diagnosis and estimate coronary heart disease danger, metabolic syndrome and calculates 4 aspect purposes such as LDL-C level.
The serum TG assay method generally can be divided into chemical method, enzyme process and chromatography 3 big classes.Early stage assay method is the difference estimation with TL and cholesterol and phosphatide.Chemical method is with the TG in the organic solvent extracting sample, remove in the extract chaff interference such as phosphatide after, with basic hydrolysis (saponification) TG, generate formaldehyde with periodate oxidation glycerine, survey formaldehyde with chromogenic reaction then.Be methylene chloride-silicic acid-variable color acid system (VanHandel-Caslson method) more accurately, this method extracting fully, can remove phosphatide and glycerine interferences, chromotropic acid developing sensitivity height, color stability, still be the internal reference method of U.S. CDC (CDC) so far.But, technical requirement various because of operation steps be high to be unsuitable for routine work and to use.Nucleic dilution/gas chromatography/mass spectrometry technology (ID/GC/MS) mainly is used as the foundation of decisive method in the frame of reference and the preparation and the definite value of reference material, this method expense costliness, and the sample preparation complexity is difficult to apply.
The serum TG level all detects with enzyme process at present nearly all clinical labororatory, though method is different, generally all comprises 3 basic steps: generate glycerine and FFA with only LPL hydrolysis TG; Then be to transform, only use a kind of enzyme as this step 1, for example glycerokinase to carry out next step reaction, perhaps generates the centre determinand with phosphoglycerolization; Be the formation of coloured dyestuff (often being quinone imines etc.) or uv-absorbing substance at last, calculate corresponding TG concentration by spectrophotometric method again.As lipoprotein lipase-phosphoglycerol oxidase-peroxidase-4-amino-antipyrine and phenol method (GPO-PAP method) etc.This method have easy fast, trace, advantage that precision is high, and high specificity, be easy to reach terminal point, the range of linearity is wide, but the method needs supporting Biochemical Analyzer to use, blood using amount big (5mL vein whole blood), professional strong operability, and can not reach instant mensuration, it is used and patient's compliance is subjected to certain restriction.
The triglyceride determination kit of Biochemical Analyzer not needing to have appearred in recent years, be used for cities and towns and community medicine, for example, the patent No. is that 200610018747.5 Chinese invention patent discloses a kind of kit that is used for diagnosing high triglyceride (FHTG), utilize the biochemical indicator of ApoA5 (ApoA5) as the FHTG diagnosis, the monoclonal antibody for preparing anti-ApoA5 is right.There is enzyme to mark the monoclonal antibody of anti-ApoA5 in the kit and monoclonal antibody, confining liquid, enzyme substrate solution, the bag of unmarked anti-ApoA5 is cushioned liquid, stop buffer, cleansing solution and ELISA Plate.With the monoclonal antibody bag of unmarked anti-ApoA5 by in ELISA Plate to catch the ApoA5 in the blood of human body, the monoclonal antibody of marking anti-ApoA5 with enzyme discern with human body blood in ApoA5, and be applied to the etiological diagnosis of hypertriglyceridemia, thereby reach effective control of angiocardiopathies such as coronary heart disease and apoplexy.Though such kit need not to buy expensive Biochemical Analyzer, but still need supporting microplate reader to use, and do not overcome the problem that blood using amount is big and can not measure immediately.
Summary of the invention
The object of the invention provides a kind of chromatography type triglyceride self-measuring system that adopts enzyme process and chromatography developing to combine to measure content of triglyceride in the blood.
For achieving the above object, the concrete technical scheme of the present invention is, a kind of chromatography type triglyceride self-measuring system comprises: a housing, be placed in the housing cavity draw the liquid test paper, the reaction test paper makes solution system and draws the liquid feeding mechanism that the liquid test paper contacts with the test paper that develops the color, a solution system and; The principal ingredient of contained solution is the superoxide enzyme solutions in the described solution system, described solution system can place in the housing cavity or housing cavity outside; Housing is provided with a blood sampling hole, is provided with one in the housing and receives pad, and described reception pad is provided with two working positions, a blood sampling position and a chromatography position; Be provided with lipoproteinesterase and background in the described reception pad, described background is selected from: glycerine, olein, contain the serum of triglyceride or contain the blood plasma of triglyceride; Described reaction test paper is fixed with lipoproteinesterase, GPO, glycerokinase, atriphos and water-soluble magnesium salt; The chromatography speed of described reaction test paper is 0.6cm/min～2.5cm/min.
In the technique scheme, described solution system can be the form that realizes the solution packing arbitrarily, for example: forms such as solution box, solution bag or solution conduit.
In the technique scheme, described liquid feeding mechanism can be arbitrarily make in the solution system solution with draw the form that the liquid test paper contacts, for example: the solution filling orifice, the solution in the solution conduit is injected by filling orifice, and draw the liquid test paper and contact; Perhaps, be provided with a sliding part that has an acupuncture portion in housing, the pulling sliding part punctures the solution bag, discharges solution and contacts with drawing the liquid test paper, etc. form.
In the technique scheme, described blood sampling is coated with the plural layer screen pack on the hole, but filtering whole blood obtains blood plasma.
In the technique scheme, described reception pad is provided with two working positions, a blood sampling position and a chromatography position, when receiving pad and be in the blood sampling position, receive pad be positioned at the hole of taking a blood sample under; When the reception pad was in the chromatography position, described reception pad was positioned at the below of drawing liquid test paper and reaction test paper, and its two ends are undertaken in the both sides test paper, and overlapping 0.5mm～1.5mm, the blood plasma volume quantitative that this reception pad is absorbed 〉=15 μ L; Because the content of triglyceride is lower in the serum, the colour developing aspect ratio when detecting on the colour developing test paper is lower, is difficult to observation, and therefore the reception pad of a constant volume at least 15 μ L is set, and increases the content of triglyceride, thus convenient observation; Accordingly simultaneously, described reception pad is fixed with 56U/cm at least
2Lipoproteinesterase (LPL) is considered the factor of economy simultaneously, and preferably, described reception pad is fixed with 112U/cm
2～224U/cm
2Lipoproteinesterase; Because whole detection may further comprise the steps:
The above-mentioned steps required time is long, and first reaction is a rate-limiting step, and therefore fixing lipoproteinesterase in receiving pad starts first reaction earlier guaranteeing the abundant reaction of triglyceride, thereby makes testing result accurate.
Equally, accordingly, described reception pad is fixed with 4 μ mol/cm
2～110 μ mol/cm
2Background, background is selected from: glycerine, olein, contain the serum of triglyceride or contain the blood plasma of triglyceride; The reason that adds background is because the content of triglyceride is lower in the serum equally, can present certain altitude on the colour developing test paper behind the adding background, is the zero graduation datum line with this place, thereby makes the peak type of colour developing more obvious, the more convenient observation of result.
In the technique scheme, be provided with a view window corresponding to the upper shell above the colour developing test paper, the view window subscript is provided with scale mark, and scale marker is arranged.
In the technique scheme, described housing proximal portion is provided with one and finishes indicator hole, contain water-soluble end point indicator on the colour developing test paper of indicator hole below, after the colour developing test paper color development finishes, the surplus solution chromatography is to finishing the indicator hole place, finish the indicator hole place and the indicator color will occur, show that detection has finished and can carry out the result to read.
In the technique scheme, the principal ingredient of the contained solution of described solution system is the superoxide enzyme solutions, peroxidase concn 〉=0.6U/mL.
In the technique scheme, described reaction test paper is fixed with lipoproteinesterase, GPO, glycerokinase, atriphos and magnesia mixture, the content 〉=28U/cm of lipoproteinesterase
2, the content 〉=4U/cm of GPO
2, the content 〉=4U/cm of glycerokinase
2Consider Eco-power factor simultaneously, preferably, the content of lipoproteinesterase is 28U/cm
2～224U/cm
2, the content of GPO is 4U/cm
2～16U/cm
2, the content of glycerokinase is 4U/cm
2～16U/cm
2
In the technique scheme, described reaction test paper is fixed with 0.1mg/cm
2～1mg/cm
2Bovine serum albumin(BSA), bovine serum albumin(BSA) can increase the stability of the reaction system on the reaction test paper on the one hand, can change the character of chromatographic test paper on the other hand, total chromatography time of conditioned reaction test paper; Total chromatography time of conditioned reaction test paper is 1 minute～5 minutes, preferred 3 minutes.
The course of work of above-mentioned chromatography type triglyceride self-measuring system is: when droplets of whole blood was added to blood sampling hole on the housing, the blood sampling hole can be filtered whole blood obtain blood plasma; Blood plasma is received pad and quantitatively absorbs; Solution in the solution box discharges, and draws the liquid test paper and by capillary action solution chromatography is successively filled up, reacts test paper and colour developing test paper to receiving; The colour developing test paper presents the bluish violet colour band of certain altitude, by the concentration of triglyceride in the colour band height indication whole blood.
Therefore, the present invention is claimed a kind of chromatography type triglyceride self-measuring method simultaneously, adopts to comprise that one receives pad, a cover and draws liquid test paper, reaction test paper and the test paper that develops the color, a solution system and and make solution system and the system of drawing the liquid feeding mechanism that the liquid test paper contacts; The principal ingredient of contained solution is the superoxide enzyme solutions in the described solution system, described solution system can place in the housing cavity or housing cavity outside; Measure triglyceride in blood content according to following steps:
(1) blood sample to be measured is added to blood sampling Kong Zhongyong and receives the pad quantitative collection;
(2) by liquid feeding mechanism the solution in the solution system is discharged, draw the liquid test paper and solution chromatography is successively filled up, reacts test paper and colour developing test paper to receiving by capillary action; According to the content that goes out the elevation measurement triglyceride at peak on the colour developing test paper;
Wherein, in the step (1), receive and wait at least 2 minutes time delay after the pad quantitative collection finishes; And be provided with lipoproteinesterase and background in the described reception pad, described background is selected from: glycerine, olein, contain the serum of triglyceride or contain the blood plasma of triglyceride; Described reaction test paper is fixed with lipoproteinesterase, GPO, glycerokinase, atriphos and water-soluble magnesium salt; The chromatography speed of described reaction test paper is 0.6cm/min～2.5cm/min.
In the technique scheme, the time delay of waiting at least 2 minutes is for elder generation starts the abundant reaction of first reaction of entire reaction with the assurance triglyceride, thereby makes testing result accurate.
Because the technique scheme utilization, the present invention compared with prior art has following advantage:
1. intrinsic a certain amount of background, lipoproteinesterase that contains triglyceride in the set reception pad of the present invention, described reaction test paper is fixed with lipoproteinesterase, GPO, glycerokinase, atriphos and water-soluble magnesium salt, and is fixed with 0.1mg/cm
2～1mg/cm
2Therefore bovine serum albumin(BSA) can realize the instant mensuration to triglyceride in blood.
2. the present invention is simple in structure, need not use by necessary instrument, and easy to use, cost is low, meets the application trend of POCT (point of care testing, the instant detection).
Description of drawings
Fig. 1 is the structural representation of the embodiment of the invention one;
Fig. 2 is the rear view of Fig. 1;
Fig. 3 is the A-A cut-open view of Fig. 1;
Fig. 4 is the B-B cut-open view of Fig. 2;
Fig. 5 is the C-C cut-open view of Fig. 1;
Structural representation when Fig. 6 is the embodiment of the invention one use;
Fig. 7 is the rear view of Fig. 6;
Fig. 8 is that Fig. 6 is at the structural representation of opening upper shell and spherical cap;
Fig. 9 is the structural representation of chromatography cap among Fig. 8;
Figure 10 is the D-D cut-open view of Fig. 6;
Figure 11 is the stereographic map behind the removal test paper among Fig. 8;
Figure 12 is the E-E cut-open view of Fig. 6;
Figure 13 is the one-piece construction synoptic diagram of chromatography type triglyceride self-measuring system among the embodiment two;
Figure 14 is the sliding part synoptic diagram of chromatography type triglyceride self-measuring system among the embodiment two;
Figure 15 is a triglyceride self-measuring comparison diagram as a result among the embodiment two;
Figure 16 is the figure as a result of no background chromatography type triglyceride self-measuring system among the embodiment three;
Figure 17 is the figure as a result that contains the background chromatography type triglyceride self-measuring system among the embodiment three;
Figure 18 is the figure as a result of no LPL chromatography type triglyceride self-measuring system among the embodiment four;
Figure 19 is the figure as a result that contains the LPL chromatography type triglyceride self-measuring system among the embodiment four;
Wherein: 1, housing; 2, chromatography cap; 3, reaction test paper; 4, colour developing test paper; 5, observation port; 6, acupuncture portion; 7, open-work; 8, draw the liquid test paper; 9, sampling portion; 10, blood sampling hole; 11, slide plate; 12, carrier; 13, shifting block; 14, skidway hole; 15, interface; 16, container cavity; 17, spherical cap; 18, spacing preiection; 19, step; 20, hole clipping; 21, lip block; 22, projection; 23, finish indicator hole; 24, solution hole; 26, delivery system; 27, solution conduit; 28, receive pad.
Embodiment
Below in conjunction with drawings and Examples the present invention is further described:
Embodiment one
Extremely shown in Figure 12 referring to Fig. 1, a kind of chromatography type triglyceride self-measuring system, comprise housing 1, chromatography cap 2 and place the reaction test paper 3 in housing 1 chamber and the test paper 4 that develops the color, have observation port 5 on the upper shell 1 at corresponding colour developing test paper 4 places, upper shell 1 subscript of observation port 5 both sides is provided with the scale mark (not shown), described housing 1 bottom is provided with acupuncture portion 6, has the open-work 7 that runs through housing 1 inner chamber on the housing 1 at this place, is provided with in housing 1 chamber at corresponding open-work 7 places and draws liquid test paper 8; The described housing 1 that draws between liquid test paper 8 and the reaction test paper 3 is outwards outstanding, constitute sampling portion 9, have the blood sampling hole 10 that connects housing 1 inner chamber on the sampling portion 9 corresponding upper shells 1, blood sampling is coated with 4 layers of screen pack (not shown) on the hole 10, the below in blood sampling hole 10, be provided with a slide plate 11 in the housing 1, the slide plate 11 of corresponding blood sampling hole 10 sides is provided with recessed carrier 12, be equipped with in the carrier 12 to receive to fill up and (do not draw, quantitatively absorb 20 μ l blood plasma), this reception pad is positioned at the below of drawing liquid test paper 8 and reaction test paper 3, its two ends are undertaken in the both sides test paper, and overlapping 1mm, slide plate 11 dorsal parts are provided with shifting block 13, and this shifting block 13 passes and is positioned on the lower house 1 and the perpendicular skidway hole of offering 14 of housing 1 bearing of trend, and the distance of described shifting block 13 slippages matches from the distance that 10 belows, hole of taking a blood sample move to the test paper place with carrier 12;
As shown in Figure 9, described chromatography cap 2 one ends are interface 15, the top of this interface 15 and described housing 1 and bottom are all blocked inserting and are closed, chromatography cap 2 other ends are provided with semisphere container cavity 16, upper surface has circular open, be provided with spherical solution bag (not shown) in the chamber, to being arranged with a spherical cap 17 by opening part; When inserting in housing 1 top, the spacing preiection 18 in the interface 15 cooperates with the step 19 on housing 1 top, and housing 1 end is limited in outside the container cavity, avoids solution bags burst (as shown in Figure 1); When chromatography cap 2 cards insert in housing 1 bottom, spacing preiection 18 and housing 1 positon of near bottom hole clipping 20 engagement connections in the interface 15, acupuncture portion 6 is positioned at container cavity 16, punctures solution bag (as shown in Figure 8); Described lower house 1 middle part is provided with lip block 21, and its bottom surface cooperates with these chromatography cap 2 container cavities 16 bottom surface levels, and housing 1 is placed horizontally on the desktop, guarantees test accuracy;
As shown in figure 11, described housing is respectively equipped with 22 of a plurality of location test paper on the madial wall about in the of 1, is used for being positioned over the location that compresses of each test paper in the housing 1;
As shown in Figure 6, described housing 1 proximal portion is provided with one and finishes indicator hole 23, after end of test (EOT), can finish the color that indicator hole 23 is seen indicator from this.
In the present embodiment:
The preparation method of described reception pad is: according to the form below formulated solution, test paper dipped in gets enzyme solutions, dip in repeatedly get after, 40 ℃ of dryings.
In the technique scheme, described reaction test paper is fixed with lipoproteinesterase, GPO, glycerokinase, atriphos and water-soluble magnesium salt, particularly, the preparation method of reaction test paper is: according to the form below formulated enzyme solutions, test paper dipped in get enzyme solutions, dip in repeatedly get after, 40 ℃ of dryings, the chromatography speed of described reaction test paper is 1.5cm/min.
The using method of above-mentioned chromatography type triglyceride self-measuring system is:
1. the horizontal positioned tester punctures finger with blood taking needle, drips one to two and bleeds in the blood sampling hole, and blood the scale mark in the hole of not taking a blood sample, receive fill up be positioned at the hole of taking a blood sample under, the blood plasma of quantitative collection 20 μ L;
2. wait for 3 minutes time delay, the pulling shifting block will receive pad and place the chromatography position, pull up the chromatography cap, cover the bottom of housing, start reaction; (shown in Fig. 6,7);
3. wait for 10-12 minute, reaction finished when indicator turned green in finishing indicator hole;
4. read the colour developing height, finding out corresponding triglyceride concentration in the table of comparisons as a result.
Embodiment two
As shown in figure 13, a kind of chromatography type triglyceride self-measuring system comprises: a housing, a delivery system 26, a solution conduit 27; Described housing comprises loam cake and lower cover, and housing is provided with a blood sampling hole 10, hole 23 is finished in a solution hole 24,, is provided with one in the housing and receives pad, a sliding part 25; Described delivery system comprises that one is placed on and draws liquid test paper, reaction test paper and colour developing test paper in the housing cavity, and described solution system can place in the housing cavity or outside the housing cavity; The principal ingredient of contained solution is the superoxide enzyme solutions in the described solution conduit, peroxidase concn 〉=0.6U/mL, described solution system can place in the housing cavity or housing cavity outside.
Described blood sampling is coated with the plural layer screen pack on the hole, but filtering whole blood obtains blood plasma; Upper shell corresponding to colour developing test paper top is provided with a view window, and the view window subscript is provided with scale mark, and scale marker is arranged; Described housing proximal portion is provided with one and finishes indicator hole, contain water-soluble end point indicator on the colour developing test paper of indicator hole below, after the colour developing test paper color development finishes, the surplus solution chromatography is to finishing the indicator hole place, finish the indicator hole place and the indicator color will occur, show that detection has finished and can carry out the result to read.
Described reception pad is provided with two working positions, a blood sampling position and a chromatography position, when receiving pad and be in the blood sampling position, receive pad be positioned at the hole of taking a blood sample under; When the reception pad was in the chromatography position, described reception pad was positioned at the below of drawing liquid test paper and reaction test paper, and its two ends are undertaken in the both sides test paper, and overlapping 1mm, and the blood plasma volume quantitative that this reception pad is absorbed is 20 μ L; The preparation method of described reception pad is: according to the form below formulated solution, test paper dipped in gets enzyme solutions, dip in repeatedly get after, 40 ℃ of dryings.
In the technique scheme, described reaction test paper is fixed with lipoproteinesterase, GPO, glycerokinase, atriphos and water-soluble magnesium salt, particularly, the preparation method of reaction test paper is: according to the form below formulated enzyme solutions, test paper dipped in get enzyme solutions, dip in repeatedly get after, 40 ℃ of dryings.
In the technique scheme, the preparation method of colour developing test paper is: adopt hydrogen bromide or CDI activated carrier, treat the preferred N-alkyl benzene of the reagent amine material of Covalent Immobilization, this reagent is with developping solution diffusion, the preferred 2-20mmol/L of concentration after fixing.Color Appearance System also comprises another component: benzothiazolone hydrazone class, the preferred 0.01-1mg/mL of concentration.
The course of work of above-mentioned chromatography type triglyceride self-measuring system is: when droplets of whole blood was added to blood sampling hole on the housing, the blood sampling hole can be filtered whole blood obtain blood plasma; Blood plasma is received pad and quantitatively absorbs; Solution in the solution box discharges, and draws the liquid test paper and by capillary action solution chromatography is successively filled up, reacts test paper and colour developing test paper to receiving; The colour developing test paper presents the bluish violet colour band of certain altitude, by the concentration of triglyceride in the colour band height indication whole blood.
The using method of above-mentioned chromatography type triglyceride self-measuring system is:
1. the horizontal positioned tester punctures finger with blood taking needle, drips one to two and bleeds in the blood sampling hole, and blood the scale mark in the hole of not taking a blood sample, receive fill up be positioned at the hole of taking a blood sample under, the blood plasma of quantitative collection 20 μ L;
2. wait for 3 minutes time delay, the pulling sliding part will receive pad and place the chromatography position, add solution, start reaction;
3. wait for 10-12 minute, reaction finished when indicator turned green in finishing indicator hole;
4. read the colour developing height, finding out corresponding triglyceride concentration in the table of comparisons as a result.
Accuracy and degree of accuracy for chromatography type triglyceride self-measuring system described in the check present embodiment, the content of triglyceride of the different blood of replication, and with the contrast of the Olympus5400 of hospital automatic clinical chemistry analyzer standard determination method, result such as following table and shown in Figure 15:
Reading card: y=0.162x-2.601 (y be TG from measured value, x is colour developing height)
Embodiment three
Relatively add the chromatography type triglyceride self-measuring system of glycerine background and the chromatography type triglyceride self-measuring system that does not add, result such as following table and Figure 16, shown in Figure 17 in the background:
The linearly dependent coefficient of no background is r=0.950, and linearly dependent coefficient is r=0.986 behind the interpolation glycerine background, therefore, adds background and can make measurement result more accurate.
Embodiment four
Relatively add the chromatography type triglyceride self-measuring system of LPL and the chromatography type triglyceride self-measuring system that does not add (be equivalent to wait for time delay and do not wait for time delay directly do not measure) on the pad, result such as following table and Figure 18, shown in Figure 19 receiving:
The linearly dependent coefficient that receives no LPL in the pad is r=0.827, and receiving the linearly dependent coefficient that contains LPL in the pad is r=0.985, therefore, adds LPL in filling up receiving, and wait for one at least the time delay of 2min can make measurement result more accurate.
Claims (7)
1. chromatography type triglyceride self-measuring system comprises: a housing, be placed in the housing cavity draw the liquid test paper, the reaction test paper makes solution system and draws the liquid feeding mechanism that the liquid test paper contacts with the test paper that develops the color, a solution system and; The principal ingredient of contained solution is the superoxide enzyme solutions in the described solution system, described solution system can place in the housing cavity or housing cavity outside; Housing is provided with a blood sampling hole, is provided with one in the housing and receives pad, and described reception pad is provided with two working positions, a blood sampling position and a chromatography position; It is characterized in that: be provided with lipoproteinesterase and background in the described reception pad, described background is selected from: glycerine, olein, contain the serum of triglyceride or contain the blood plasma of triglyceride; Described reaction test paper is fixed with lipoproteinesterase, GPO, glycerokinase, atriphos and water-soluble magnesium salt; The chromatography speed of described reaction test paper is 0.6cm/min～2.5cm/min.
2. according to the described chromatography type triglyceride self-measuring system of claim 1, it is characterized in that described blood sampling is coated with the plural layer screen pack on the hole.
3. according to the described chromatography type triglyceride self-measuring system of claim 1, it is characterized in that, when receiving pad and be in the blood sampling position, receive pad be positioned at the blood sampling hole under; When receiving pad and be in the chromatography position, described reception pad is positioned at the below of drawing liquid test paper and reaction test paper, and its two ends are undertaken in the both sides test paper, and overlapping 0.5mm～1.5mm, and this receives the quantitatively blood plasma volume 〉=15 μ L of absorption of pad institute; Described reception pad is fixed with 〉=56U/cm
2Lipoproteinesterase; Described reception pad is fixed with 4 μ mol/cm
2～110 μ mol/cm
2Background, background is selected from: glycerine, olein, contain the serum of triglyceride or contain the blood plasma of triglyceride.
4. according to the described chromatography type triglyceride self-measuring system of claim 3, it is characterized in that the principal ingredient of contained solution is the superoxide enzyme solutions in the described solution system, peroxidase concn 〉=0.6U/mL.
5. according to the described chromatography type triglyceride self-measuring system of claim 1, it is characterized in that described reaction test paper is fixed with lipoproteinesterase, GPO, glycerokinase, atriphos and water-soluble magnesium salt, the content 〉=28U/cm of lipoproteinesterase
2, the content 〉=4U/cm of GPO
2, the content 〉=4U/cm of glycerokinase
2
6. according to the described chromatography type triglyceride self-measuring system of claim 1, it is characterized in that described reaction test paper is fixed with 0.1mg/cm
2～1mg/cm
2Bovine serum albumin(BSA), total chromatography time is 1 minute～5 minutes.
7. chromatography type triglyceride self-measuring method adopts to comprise that a blood sampling hole, receives pad, a cover and draws liquid test paper, reaction test paper and the test paper that develops the color, a solution system and and make solution system and the system of drawing the liquid feeding mechanism that the liquid test paper contacts; The principal ingredient of contained solution is the superoxide enzyme solutions in the described solution system, described solution system can place in the housing cavity or housing cavity outside; Described reception pad is provided with two working positions, a blood sampling position and a chromatography position, when receiving pad and be in the blood sampling position, receive pad be positioned at the hole of taking a blood sample under; When the reception pad was in the chromatography position, described reception pad was positioned at the below of drawing liquid test paper and reaction test paper, and its two ends are undertaken in the both sides test paper; Measure triglyceride in blood content according to following steps:
(1) will receive pad and place the blood sampling position, and blood sample to be measured will be added to blood sampling Kong Zhongyong receive the pad quantitative collection;
(2) will receive pad and place the chromatography position, the solution in the solution system be discharged, and draw the liquid test paper and solution chromatography is successively filled up, reacts test paper and colour developing test paper to receiving by capillary action by liquid feeding mechanism; According to the content that goes out the elevation measurement triglyceride at peak on the colour developing test paper;
It is characterized in that, in the step (1), receive and wait at least 2 minutes time delay after the pad quantitative collection finishes; And be provided with lipoproteinesterase and background in the described reception pad, described background is selected from: glycerine, olein, contain the serum of triglyceride or contain the blood plasma of triglyceride; Described reaction test paper is fixed with lipoproteinesterase, GPO, glycerokinase, atriphos and water-soluble magnesium salt; The chromatography speed of described reaction test paper is 0.6cm/min～2.5cm/min.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN107356767A (en) * | 2017-07-28 | 2017-11-17 | 武汉市安友泽瑞科技有限公司 | Interstitialcellstimulating hormone (ICSH) test strips analyzer |
| CN107389954A (en) * | 2017-07-28 | 2017-11-24 | 武汉市安友泽瑞科技有限公司 | Interstitialcellstimulating hormone (ICSH) ELISA test strip pen |
| CN107690577A (en) * | 2015-06-05 | 2018-02-13 | 荷兰联合利华有限公司 | Method of testing for the specific composition in antiperspirant and wetting agent cosmetic composition |
| CN110007076A (en) * | 2019-05-13 | 2019-07-12 | 无锡博慧斯生物医药科技有限公司 | A kind of whole blood sample detection device and its detection method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107690577A (en) * | 2015-06-05 | 2018-02-13 | 荷兰联合利华有限公司 | Method of testing for the specific composition in antiperspirant and wetting agent cosmetic composition |
| CN107064123A (en) * | 2017-01-03 | 2017-08-18 | 长沙中生众捷生物技术有限公司 | The detection reagent of triglycerides and the Test paper of triglycerides |
| CN107356767A (en) * | 2017-07-28 | 2017-11-17 | 武汉市安友泽瑞科技有限公司 | Interstitialcellstimulating hormone (ICSH) test strips analyzer |
| CN107389954A (en) * | 2017-07-28 | 2017-11-24 | 武汉市安友泽瑞科技有限公司 | Interstitialcellstimulating hormone (ICSH) ELISA test strip pen |
| CN110007076A (en) * | 2019-05-13 | 2019-07-12 | 无锡博慧斯生物医药科技有限公司 | A kind of whole blood sample detection device and its detection method |
| CN110007076B (en) * | 2019-05-13 | 2024-02-27 | 无锡博慧斯生物医药科技有限公司 | Whole blood sample detection device and detection method thereof |
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